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Magnetic solid-phase extraction agent based on covalent organic framework material and preparation method and application thereof

A technology of covalent organic framework and solid phase extraction agent, applied in chemical instruments and methods, other chemical processes, ion exchange, etc., to achieve the effect of less consumption of organic solvents, good enrichment effect, and short time

Active Publication Date: 2017-12-01
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no report on the separation and enrichment of glycoproteins using covalent organic framework material COF-5 as a solid-phase extraction adsorbent.

Method used

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  • Magnetic solid-phase extraction agent based on covalent organic framework material and preparation method and application thereof
  • Magnetic solid-phase extraction agent based on covalent organic framework material and preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0029] (1) Preparation of ferroferromagnetic nanoparticles: 0.7 g FeCl 2 4H 2 O was dissolved in water, and KNO was added sequentially under the condition of nitrogen gas 3 Solution (2.0 M) 10 mL and NaOH solution (1.0 M) 10 mL, then add 1.7 g 50% PEI solution, then put the mixed solution at 90 °C for 2 h, use a magnet to separate the magnetic nanoparticles from the solution , the collected particles were washed several times with water and absolute ethanol, and dried in an oven at 60°C for use.

[0030] (2) Preparation of magnetic nanoparticles modified with covalent organic framework materials: Weigh 20 mg of dopamine, dissolve in 20 mL of Tris buffer with a concentration of 10 mmol / L, adjust the pH to 8.5, expose to the air and stir until the solution becomes Light reddish brown, weigh 1.5 g of magnetic nanoparticles and add them to the prepared dopamine solution, react at 40°C for 1 h, separate the magnetic nanoparticles from the solution with a magnet, wash the collect...

Embodiment 2

[0034] (1) Preparation of ferroferromagnetic nanoparticles: 0.7 g FeCl 2 4H 2 Dissolve O in water, add 10 mL of KNO3 solution (2.0 M) and 10 mL of NaOH solution (1.0 M) in sequence under nitrogen gas, then add 1.7 g of 50% PEI solution, and then put the mixed solution at 95°C for reaction After 1.5 h, the magnetic nanoparticles were separated from the solution with a magnet, the collected particles were washed with water and absolute ethanol several times, and dried in an oven at 60 °C for use.

[0035](2) Preparation of magnetic nanoparticles modified with covalent organic framework materials: Weigh 20 mg of dopamine, dissolve in 20 mL of Tris buffer with a concentration of 10 mmol / L, adjust the pH to 8.5, expose to the air and stir until the solution becomes Light reddish brown, weigh 1.5 g of magnetic nanoparticles and add them to the prepared dopamine solution, react at 40°C for 1 h, separate the magnetic nanoparticles from the solution with a magnet, wash the collected p...

Embodiment 3

[0039] (1) Preparation of ferroferromagnetic nanoparticles: 0.7 g FeCl 2 4H 2 O was dissolved in water, and KNO was added sequentially under the condition of nitrogen gas 3 Solution (2.0 M) 10 mL and NaOH solution (1.0 M) 10 mL, then add 1.7 g 50% PEI solution, then put the mixed solution at 95°C for 1.5 h, and use a magnet to separate the magnetic nanoparticles from the solution , the collected particles were washed several times with water and absolute ethanol, and dried in an oven at 60°C for use.

[0040] (2) Preparation of magnetic nanoparticles modified with covalent organic framework materials: Weigh 20 mg of dopamine, dissolve in 20 mL of Tris buffer with a concentration of 10 mmol / L, adjust the pH to 9, expose to the air and stir until the solution becomes Light reddish brown, weigh 1 g of magnetic nanoparticles and add them to the prepared dopamine solution, react at 45°C for 0.5 h, separate the magnetic nanoparticles from the solution with a magnet, and wash the ...

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Abstract

The invention relates to a magnetic solid-phase extraction agent based on a covalent organic framework material and a preparation method and application thereof. The magnetic solid-phase extraction agent comprises a ferriferous oxide magnetic nanoparticle core, a polydopamine layer and a covalent organic framework material COF-5 layer, and the polydopamine layer and the covalent organic framework material COF-5 layer are sequentially coated outside the core. A method includes: adding functionalized magnetic nanoparticles into a to-be-treated glycoprotein sample, sufficiently stirring for extraction, using a magnet to separate the magnetic nanoparticles from a solution, adding elution liquid to elute off the glycoprotein from the magnetic nanoparticles, and measuring. The covalent organic framework material can selectively enrich glycoprotein and effectively separate the glycoprotein from impurities, so that the method has good extraction effect on the glycoprotein. The solid-phase extraction method is simple in operation step, short in time, high in recycling rate, low in organic solvent consumption and suitable for enrichment and measurement of the glycoprotein in complex biological samples like plasma and urine.

Description

technical field [0001] The invention relates to a magnetic solid-phase extraction agent, in particular to a magnetic solid-phase extraction agent based on a covalent organic framework material and a preparation method and application thereof. Background technique [0002] Glycoprotein is a kind of protein containing oligosaccharide chains, and the changes of its concentration and sugar chains are often related to the occurrence and development of some tumor diseases. Therefore, early diagnosis and prognosis evaluation of diseases can be realized by monitoring the changes of some glycoproteins. Glycoprotein-containing samples are usually complex biological samples such as serum, saliva, and urine, which are easily interfered by various substances during analysis. Therefore, before glycoprotein analysis, it is usually necessary to separate and enrich them. [0003] Solid-phase extraction technology is a commonly used sample pretreatment technology at present. It selectively a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/30B01D15/08
Inventor 陈子林王榕
Owner WUHAN UNIV
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