26results about How to "High enrichment efficiency" patented technology

This invention discloses a deep defluorination agent and its application method, specifically relating to the field of defluorination agents (GMS-FS series products). It includes a valence-state switching active phase, a reaction interface building component, a dynamic electronic regulation component, and a structure adjustment and protection component. The valence-state switching active phase provides a reversible valence-state transition reaction center, achieving specific capture of fluoride ions through an electron trapping effect when interacting with them. The reaction interface building component forms a polar gradient interface layer on the surface of the defluorination agent, guiding fluoride ions to migrate towards the active center and locally enrich them, thereby improving defluorination efficiency. By constructing a rare metal valence-state switching active phase with La2O3 and UO2 as its core, combined with a polar gradient interface guiding layer and a dynamic electronic regulation mechanism, preferential migration and specific capture of fluoride ions are induced in a multi-ion coexistence environment, achieving stable deep defluorination under high salinity and highly competitive ion conditions.

The invention relates to the technical field of bioengineering, in particular to a targeting nanoparticle for treating acute respiratory distress syndrome as well as a preparation method and application of the targeting nanoparticle. Comprising the following steps: (1) combining MP peptide with DSPE-PEG2000-MAL through a maleimide-mercaptan reaction, dialyzing the obtained conjugate, washing, and freeze-drying, so as to obtain a DSPE-PEG2000-MP conjugate; and (2) mixing the conjugate, lipid, a pharmaceutical preparation and a solvent, removing the solvent to obtain a lipid film, hydrating the lipid film, and performing membrane extrusion to obtain the nanoparticles. The invention not only provides a novel efficient and safe ARDS treatment scheme, but also provides an innovative mode for the design of a nano-drug delivery system based on cell mediation. And due to the dual targeting capability, excellent anti-inflammatory efficiency and good biocompatibility, the nano-material has a wide prospect in clinical application.

The utility model discloses a new pollution enrichment device, including workstation and installing the rotary base of workstation top, install the material taking subassembly on the rotary seat, the top outer wall of workstation still is equipped with independent processing subassembly, the one side of workstation bottom outer wall is equipped with the link frame, is equipped with the medium cylinder of equidistance distribution on the link frame, the independent processing subassembly includes the installation groove setting in the top outer wall both sides of workstation, and the bottom inner wall of two installation grooves all is connected with the toothed disc through bearing, the bottom outer wall of installation groove still is equipped with the gear group of equidistance distribution, and the linkage cooperation is formed between toothed disc and gear group. The utility model discloses a new pollution enrichment device is equipped with the independent processing subassembly of double position, has the technical effect of improving the new pollution enrichment efficiency.

This invention relates to a method for enhanced cascade separation and resource utilization of calcium-free roasted chromium slag through reducing alkaline roasting. The specific steps are as follows: Calcium-free roasted chromium slag, a reducing agent, and an alkaline reagent are mixed to obtain a chromium slag mixture; the chromium slag mixture is placed at a first temperature and held for a first time under an inert atmosphere, causing the iron oxides in the chromium slag mixture to be reduced, and the aluminum and chromium oxides to be sodium-treated, while inhibiting the reduction of chromium oxides, resulting in a reduced chromium slag mixture; the reduced chromium slag mixture is filtered to obtain filter residue and an aluminum-enriched filtrate, which can be used for alumina production and the recovery of the alkaline reagent; under an inert atmosphere, the filter residue is placed at a second temperature and held for a second time. Due to the separation of aluminum in the filter residue, metallic iron can be efficiently separated by melting, and chromium can be efficiently enriched in the molten residue. This invention can efficiently achieve the cascade resource utilization of iron, chromium, and aluminum components in calcium-free roasted chromium slag.

This invention belongs to the field of pharmaceutical technology for the treatment and prevention of oral diseases, and particularly relates to a porphyrin and ferrocene derivative, nanoparticles, preparation methods, and applications of synergistic photothermal and acoustic therapy for oral diseases. The porphyrin and ferrocene derivative are formed by organically combining tetraaminophenylporphyrin and diformaldehyde ferrocene through imine dynamic covalent bonds. This invention, through innovative raw material coupling methods and functional integration design, synergistically achieves PTT-SDT dual therapy, providing a new and superior technical solution for precise, efficient, and low-drug-resistance treatment of deep oral bacterial-fungal biofilm infections.

This application discloses a composite catalyst for contact electrocatalytic removal of water pollutants, its preparation method, and its application. This composite catalyst, when applied to contact electrocatalytic removal of water pollutants, can continuously generate charge transfer and form a stable, strong interfacial electric field under mechanical disturbance conditions, efficiently degrading organic pollutants in water. It also exhibits good stability and can be reused multiple times. The composite catalyst comprises a TPB-DVA covalent organic framework material and a fluorocarbon polymer coated on the surface of the TPB-DVA covalent organic framework material. The TPB-DVA covalent organic framework material and the fluorocarbon polymer are interfacially bonded through hydrogen bonds.

The application discloses a Bifidobacterium lactis specific protein antibody, an antibody conjugated magnetic bead and application. The antibody is a monoclonal antibody BL1, comprising a heavy chain variable region and a light chain variable region, and the amino acid sequences of the heavy chain variable region and the light chain variable region are shown in SEQ ID NO: 3 and 4 respectively. The antibody conjugated magnetic bead is coupled by the monoclonal antibody BL1 and activated carboxyl magnetic beads. The antibody conjugated magnetic bead can specifically enrich Bifidobacterium lactis, has high enrichment efficiency, simple enrichment operation, and can be applied to identification of metabolic products of Bifidobacterium lactis, and can be used for qualitative and quantitative analysis.

The invention relates to a method for enriching and recovering rhodium from organic waste liquid containing rhodium acetylacetonate, and belongs to the technical field of precious metal secondary resource recovery. The method comprises the following steps: carrying out reduced pressure evaporation on an organic waste liquid containing rhodium acetylacetonate, separating, condensing and recovering low-boiling-point organic components to obtain a distillation residual liquid containing rhodium acetylacetonate; adding a water phase complex breaking and phase inversion system into the distillation residual liquid, stirring and reacting to break complex of rhodium acetylacetonate and promote rhodium to transfer into a water phase, and separating an organic phase from the water phase to obtain a rhodium-containing water phase; adjusting the rhodium-containing water phase by using an alkaline solution under a stirring condition, stably controlling the pH value to be 6-9, simultaneously dropwise adding a sulfuration precipitant solution, reacting to generate rhodium-containing sulfide precipitates, carrying out heat preservation and aging treatment for 0.5-4 hours, carrying out solid-liquid separation to obtain rhodium enriched products, and sequentially washing and drying the rhodium enriched products to obtain the rhodium enriched products. The method is simple in process and mild in condition, and efficient phase inversion and enrichment recovery of rhodium in the rhodium acetylacetonate-containing organic waste liquid can be realized.

The invention discloses a high-affinity nano antibody for resisting a nervous centralis specific protein S100beta or an antigen binding fragment of the high-affinity nano antibody and application of the high-affinity nano antibody, and belongs to the technical field of antibodies. The amino acid sequence of the nano antibody is as shown in SEQ ID NO: 2, and the nucleotide sequence of the nano antibody is as shown in SEQ ID NO: 1. The invention provides a high-affinity antibody with EC50 reaching 4.0 nM and a recombinant expression scheme capable of realizing large-scale production, and provides a high-affinity and low-cost S100beta nano antibody by reasonably designing a four-round screening strategy.

The utility model provides a protein enrichment device which comprises a heat preservation shell and a test tube rack, the test tube rack is arranged in the heat preservation shell, and a gap is formed between the outer wall of the test tube rack and the inner wall of the heat preservation shell; a plurality of test tube channels for placing test tubes are transversely arranged in the test tube rack; and a test tube rack body is arranged among the test tube channels and is made of a heat-conducting material. According to the device provided by the utility model, the separation between the target protein and the target protein antibody beads caused by liquid dripping is reduced, so that the enrichment efficiency of the protein is effectively improved.

The invention provides a nano preparation for preparing a medicine for inhibiting prostatic cancer and a preparation method of the nano preparation, and relates to the technical field of nano medicinal preparations. The nano preparation comprises the following components in parts by mass: traditional Chinese medicine components and a nano carrier, the traditional Chinese medicine component comprises 15-45 parts of a Chinese yam extract, 5-15 parts of a madder extract, 8-20 parts of cuttlebone superfine powder, 10-25 parts of dragon bone superfine powder and 10-25 parts of oyster superfine powder; the nano-carrier comprises 5-20 parts of phospholipid, 2-10 parts of cholesterol, 1-5 parts of fatty acid, 3-15 parts of polyethylene glycol and 1-5 parts of a surfactant. The average particle size of the nano preparation is 80-150 nm, the polydispersity index PDI is smaller than or equal to 0.30, and the encapsulation efficiency is larger than or equal to 75%. According to the invention, active ingredients of traditional Chinese medicines are combined with a nanotechnology, so that the bioavailability and the tumor targeting property are remarkably improved, the pH-responsive release characteristic is achieved, a remarkable inhibition effect on various prostate cancer cell lines is shown, tumor growth can be effectively inhibited, metastasis can be reduced, and a good application prospect is achieved.

This invention provides the application of a siRNA composition targeting LOC157273 in the preparation of drugs for treating non-alcoholic steatohepatitis (NASH) and liver fibrosis, belonging to the field of biomedical technology. The siRNA of this invention directly corrects the hyperlipidemic hepatic lipid synthesis, insulin resistance, and inflammatory activation caused by abnormal expression of PPP1R3B by specifically silencing the key fibrosis driver gene LOC157273. This fundamentally blocks the activation of hepatic stellate cells and excessive extracellular matrix deposition, achieving precise reversal of the fibrosis process and blocking the occurrence of NASH. The siRNA delivery system is optimized through liver-targeted modification, improving liver enrichment efficiency and reducing off-target effects caused by systemic distribution. It exhibits strong targeting, directly acting on upstream regulatory genes of fibrosis and NASH. Simultaneously, it silences multiple synergistic pathogenic gene sites, enhancing therapeutic efficacy and providing a highly efficient and safe novel intervention for liver fibrosis and NASH.

The invention relates to a nano drug delivery system for targeted removal of liver aging cells and regulation and control of oxidative stress as well as a preparation method and application of the nano drug delivery system. The nano drug delivery system comprises cerium-doped zinc-based metal organic framework nanoparticles, a drug loaded in a hollow mesoporous structure of the nanoparticles, and a bone marrow mesenchymal stem cell membrane modified on the surfaces of the nanoparticles. According to the invention, a nano drug delivery system for breaking an'aging-inflammation 'cycle is constructed on the basis of an interaction relationship between aging cells and the non-alcoholic fatty liver disease, and the enrichment efficiency and the residence time of the nano drug delivery system at a non-alcoholic steatohepatitis disease part can be improved; the hollow mesoporous metal organic framework nano particles with the effects of efficiently removing active oxygen and being high in drug loading rate are used for loading drugs, the targeting performance on liver senescent cells, the synergistic effect and safety of removing senescent cells and adjusting the inflammatory environment are improved from the two links of drug delivery and release, and liver lipid metabolism and the inflammatory environment are improved.

The application discloses an ultrasonic driving nanorobot and a preparation method and application thereof, and the preparation method comprises the following steps: reacting Pd NPs, 4-mercapto phenylacetic acid and polyacrylic acid, adding tetraethyl orthosilicate and ammonia, and stirring to obtain PdSiO2; reacting titanium chloride solution, sodium bicarbonate solution and PdSiO2, and performing amino modification to obtain NH2-TiO2-Pd-SiO2; connecting COOH-PEG-TPP and COOH-PEG-RGD to the surface of NH2-TiO2-Pd-SiO2, and removing SiO2; the nanorobot can penetrate the dense extracellular matrix in a tumor microenvironment and precisely target a diffuse lesion, and further has the functions of catalytically generating active oxygen free radicals and regulating a tumor microenvironment, so that the oxidation-reduction / metabolic balance of the TME is changed, immunogenic cell death is induced, and the anti-tumor immune response is further enhanced.

This utility model discloses a low-temperature enrichment device for electroplating cleaning solutions, comprising an evaporation chamber with a steam vent at the top, a liquid inlet on the upper side wall, and a liquid outlet at the bottom. A vacuum pump is connected to the side of the evaporation chamber to drive the low-temperature evaporation process, and the inner side wall is covered with a titanium liner to enhance corrosion resistance. A multi-stage activated carbon adsorption layer with varying pore sizes is installed at the bottom of the evaporation chamber, consisting of a perforated shell, filter cloth, and activated carbon from the outside in, for efficient ion enrichment. A backflushing device is installed directly opposite the liquid inlet to periodically flush away residues from the inner wall. A removable cover is connected to the top via a flange, and a puller groove design on the top of the activated carbon adsorption layer allows for quick replacement. The device significantly reduces energy consumption for electroplating cleaning solution treatment through low-temperature evaporation combined with activated carbon adsorption. The combination of the backflushing device and the titanium liner enhances the durability of the equipment, and the modular structure simplifies maintenance. It is suitable for low-cost ion enrichment needs such as those for roller electroplating cleaning solutions.

The application belongs to the technical field of virus DNA enrichment. More particularly, it relates to a method and a kit for enriching low-concentration pathogen nucleic acid in water bodies. The enrichment cracking solution and the eluent constructed by specific proportion produce synergistic effect, efficiently crack the pathogen, and promote the target nucleic acid to be directionally adsorbed on the magnetic beads, so that the enrichment efficiency and detection sensitivity are significantly improved, the originally undetectable or weakly positive low-load samples are stably detected, and the false negative rate is greatly reduced. The enrichment reagent provided by the application is simple to prepare, the water sample does not need complex pretreatment, and the sample treatment and enrichment operation can be completed integrally on site. The method is excellent in anti-matrix interference, process simplification, reagent saving, operation speediness and on-site applicability, overcomes the technical bottleneck that low-load pathogens and nucleic acids easily cause false negatives in detection, helps the breeding farm to take disease prevention and control measures in time, and has good application prospect and value.

This utility model discloses an odor sample preparation device, including an odor source storage chamber and bases disposed at the four corners of the lower side of the odor source storage chamber. The odor source storage chamber has a storage space inside, and an anti-volatility sealing cover is provided on the rear exterior of the storage space. A temperature control module is disposed inside the upper rear left side of the odor source storage chamber, a suction pump is disposed inside the left side of the odor source storage chamber, and a controller is disposed on the upper right front side of the odor source storage chamber. This device supports the rapid replacement of different types of adsorption materials (such as activated carbon and molecular sieves) to meet the directional enrichment needs of diverse odor samples (such as explosives, plant fragments, food, etc.), avoiding the limitations of a single material. The temperature control module is electrically connected to the external heating structure and can flexibly adjust the heating mode according to the odor source form (solid or liquid), improving the volatile efficiency of odor molecules while supporting standardized and streamlined operation.

The invention belongs to the technical field of biological engineering, and particularly relates to an engineering strain for producing cytidine based on a mini-forming system, a method and application, the engineering strain takes escherichia coli for producing cytidine as a starting strain, expression of diaphragm inhibition protein coding genes is knocked down or inactivated, and the engineering strain is obtained. Or overexpression of cell septum component coding genes or cell division topology specific factors is achieved. By taking biological membrane fermentation as a core and depending on the low-cost characteristic of the carrier, the cell stability is improved, the environmental stress resistance is enhanced, the metabolic activity is prolonged, and the sustainable requirements of industrial production are met. Meanwhile, the fermentation microenvironment can be improved through pH adjustment in the middle stage of fermentation, the cytidine yield is further increased, and a foundation is laid for follow-up industrialized large-scale production of cytidine through fermentation tank enlarged culture.

The invention belongs to the technical field of biological medicines, and particularly relates to vitexin-probucol self-assembled nanoparticles as well as a preparation method and application thereof. The nanoparticle is formed by self-assembly of vitexin and probucol, and the mass ratio of vitexin to probucol is 2: (1-5). The nano particles are prepared by adopting a nano precipitation method, and the method specifically comprises the following steps: mixing vitexin and probucol, and dissolving and dispersing in a good solvent to obtain a mixed solution; and adding the obtained mixed solution into a poor solvent, and promoting the vitexin and probucol to be self-assembled to form the nanoparticles by utilizing a solvent replacement effect. The nanoparticles have the advantages of high drug loading capacity, synergistic anti-inflammation and anti-oxidation, long-acting circulation and the like, and can effectively treat atherosclerosis, reduce side effects and improve the treatment effect and safety.

The application discloses a method for preparing a cell block by using mucin emulsifier and application thereof, and the preparation method comprises the following steps: centrifuging a non-gynecological sample to obtain a first separation liquid, adding the first separation liquid into a formaldehyde-alcohol solution to centrifuge to obtain a second separation liquid, and adding the second separation liquid into mucin emulsifier to centrifuge to obtain a cell block. The preparation method is simple, the manufacturing cost is low, the cell block has high enrichment efficiency, cell loss is small, and the mucin emulsifier does not affect cell activity. The cell block prepared by using the mucin emulsifier has the following advantages: the appearance of the section of the cell block is complete, the section has a regular circular structure, the porosity is moderate, and the thickness is uniform; the application effect of the section in two kinds of staining is good: the hematoxylin-eosin staining has a clear background and clear contrast, cells are uniformly distributed in a circular area, cell morphology presents a stereoscopic effect, and the excellent rate of the stained section is 98.94%; the immunohistochemical staining has a clean background, and structures and cells are specifically dyed, and the excellent rate of the stained section is 99.30%, so that the cell block has a wide application prospect.

This utility model belongs to the technical field of industrial nitrogen generation equipment, and aims to provide a high-efficiency nitrogen enrichment device for nitrogen generators. It includes a nitrogen generator adsorption tower, with an upper end cap at the top. A nitrogen collecting diffuser is located inside the upper end cap, with its lower end connected to an umbrella-shaped nitrogen honeycomb enricher and its upper end connected to an upper end cap flange. The upper end cap flange is bolted to a flange cover plate. A nitrogen outlet pipeline is connected to the side of the upper end cap, with a nitrogen outlet at the top. This utility model features a simple structure, high enrichment efficiency, and long service life of the molecular sieve. By using an umbrella-shaped nitrogen honeycomb enricher inside the adsorption tower, it improves nitrogen enrichment efficiency, ensures the molecular sieve is tightly compressed and will not break, and can be widely used in the production technology of the upper end cap of nitrogen generator adsorption towers.

This invention belongs to the field of industrial solid waste resource utilization technology, and relates to a method for enriching titanium dioxide in high-titanium blast furnace slag, comprising: Step S10: crushing and pretreating the high-titanium blast furnace slag to obtain fine blast furnace slag powder; Step S20: mixing the fine blast furnace slag powder with an acid solution and carrying out an acid leaching reaction to obtain a reaction slurry; Step S30: adding an alkaline substance to the reaction slurry to adjust the pH value of the slurry to 6-8, converting the silica colloids in the slurry into amorphous silica particles, completing the colloidal regulation; Step S40: performing flotation to remove impurities from the colloidal regulated slurry, removing calcium sulfate; Step S50: filtering and washing the flotation slurry to remove soluble impurities, obtaining filter residue; Step S60: drying the filter residue to obtain a product rich in titanium dioxide. This method overcomes the defects of existing dilute sulfuric acid leaching methods for enriching titanium dioxide in high-titanium blast furnace slag, such as silica colloid interference, difficult filtration, and low enrichment efficiency.

This invention relates to the field of biomedical technology and provides a method for preparing uracil-modified gold nanoconjugated materials and their applications. The method includes the following steps: in the presence of a reducing agent, a uracil ligand solution is slowly added to a gold precursor solution for a controlled reduction reaction to form gold nanoconjugated materials with an average particle size ≤2 nm; a thiolated uracil derivative is added to the dispersion of the obtained gold nanoconjugated materials, and the pH of the reaction system is adjusted to alkaline, so that the thiolated uracil derivative is bound to the surface of the gold nanoconjugated materials through Au-S bonds, thereby obtaining uracil-modified gold nanoconjugated materials. This material possesses ultra-small size, pH-responsive surface, and selective uptake characteristics by pathogens, enabling it to efficiently penetrate periodontal biofilms and achieve efficient clearance and virulence blocking against periodontal pathogens by interfering with bacterial proton kinetic potential and inhibiting the secretion of virulence factors.

The invention discloses a vagus nerve double-targeting delivery carrier as well as a preparation method and application thereof. According to the vagus nerve double-targeting delivery carrier, the surface of a nano carrier is simultaneously modified with a first targeting ligand capable of being combined with a nicotinic acetylcholine receptor on the surface of vagus nerve cells and a second targeting ligand capable of being specifically combined with a transient receptor potential channel. The enrichment efficiency and the spatial selectivity of the photoresponse nano-carrier in the vagus nerve area are effectively improved through a targeting delivery strategy of lymphatic path guidance and double receptor molecule recognition, the specific targeting of the photoresponse nano-carrier to the vagus nerve is enhanced, and in the follow-up NIR light irradiation process, the photoresponse nano-carrier has a good application prospect. Effective activation of the vagus nerve can be realized under lower optical power density, non-specific stimulation to surrounding non-target tissues is remarkably reduced, and safety and accuracy of light regulation of the vagus nerve are improved.

The invention belongs to the technical field of pharmaceutical preparations, and discloses a nanomaterial-mediated ankylosing spondylitis treatment preparation for specifically inhibiting MSC (mesenchymal stem cell) osteogenesis abnormality and application of the nanomaterial-mediated ankylosing spondylitis treatment preparation, and the technical scheme is characterized in that the preparation comprises a mannose-modified mesoporous silica nano-drug delivery system and Ikbkb siRNA (small interfering Ribonucleic Acid). The invention discloses a new mechanism that a tmTNF reverse signal drives pathological osteogenesis through a PSMA1-NF-kappa B axis under the stimulation of sTNFR I, and a mannose modified Man-MSN nano delivery system is constructed, so that active targeting on AS focus macrophages is realized; and by specifically knocking down Ikbkb, the key link of immune-osteogenesis transformation can be specifically blocked on the premise of not influencing the overall inflammation level.

The invention belongs to the technical field of organic pesticide biotoxicity detection, and particularly relates to a method for fluorescence detection of ametryn biotoxicity by using oxyspora clostridium and application. According to the method, Anfule is used as a sensitizer, oxyspora clostridium is used as a sensitive indicator organism, and chlorophyll fluorescence kinetic parameters of the oxyspora clostridium are used for detecting the biotoxicity of ametryn. The main components of Anfule are lecithin and vitamin E. The method can rapidly and sensitively detect the ametryn biotoxicity, solves the problems of long determination period and low sensitivity of the ametryn biotoxicity detection method in the prior art, and has important significance for realizing timely early warning and risk assessment of ametryn environmental pollution.