65results about How to "High enrichment efficiency" patented technology

According to the online enrichment and automatic sorting device and method for deep-sea microorganisms in the high-pressure environment, multi-stage enrichment is carried out by simulating the high-pressure and special-temperature environment of deep sea, and meanwhile, based on environment index changes observed online in the enrichment device, the deep-sea microorganisms in the high-pressure environment can be automatically sorted; the optimal time point of microbial passage is automatically judged, and a culture is transferred to a next-stage enrichment device, so that the problems that in a high-pressure environment, microbial enrichment is difficult to transfer at the optimal time point, manual operation is complicated and the like are effectively solved, and the early-stage enrichment efficiency of obtaining a single species of deep-sea microorganisms is improved; besides, automatic streak separation and automatic secondary sorting of the enriched substances are carried out in a culture medium in a high-pressure environment, and specific single colonies are automatically transferred to a pure culture kettle, so that the problems of overlapping of single colonies, low separation degree, complicated manual operation, difficulty in automatic secondary sorting in the high-pressure environment and the like in an existing high-pressure sorting technology are solved; and the high-pressure environment deep-sea microorganism sorting efficiency is greatly improved.

The invention provides a preparation method of a Janus material for simultaneous screening of protein phosphorylation and palmitoylation modification, and belongs to the technical field of magnetic material synthesis. The method comprises the following steps: firstly, preparing MCeO2; the preparation method comprises the following steps: mixing MCeO2, hexadecyl trimethyl ammonium bromide and ammonia water to obtain a mixed solution, adding 1, 2-bis (triethoxysilane) ethane into the mixed solution for reaction to obtain a product, and dispersing the product into an NH4NO3 solution for reflux reaction to obtain Janus MCP; the preparation method comprises the following steps: reacting Janus MCP with a silane coupling agent, dissolving aminated Janus MCP and 4-ethylene sulfonyl benzoic acid in a buffer solution containing EDC and NHS, and reacting to obtain the Janus material. The preparation method is reliable, the material performance is stable, and the enrichment efficiency of the magnetic Janus on target peptide fragments (phosphorylated peptide fragments and palmitoylated peptide fragments) is remarkably improved.

The invention provides a composition for improving sleep quality as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: performing supercritical fluid extraction on lavender, violet, rose and agilawood to obtain extracted oil, performing solid enzymolysis, performing immobilized ionic liquid extraction, and performing desorption to obtain extracted oil, and uniformly mixing the extracted oil with the extracted oil and a penetration enhancer to obtain the composition for improving the sleep quality. The prepared extract oil and the extract oil are mixed, and the penetration enhancer is added, so that the prepared composition for improving the sleep quality enters a human body through skin absorption, achieves the effects of regulating qi activity of viscera and regulating yin and yang of the body, has the effects of soothing the nerves, helping sleep, prolonging the sleep time, improving the sleep effect and relieving nervous mood, and has a wide application prospect.

The invention relates to the field of extraction and purification of active substances of natural products, and discloses plant active ingredients in camphor tree seed kernels and a separation and purification method thereof.The method comprises the steps that fresh camphor tree seed kernels and water are mixed, wet-process superfine grinding is conducted through a colloid mill at the room temperature, centrifugal separation is conducted to obtain a middle-layer water phase, and the middle-layer water phase is statically or dynamically adsorbed through HJ-18 macroporous resin; and desorbing with 80% ethanol, collecting a desorption solution, carrying out rotary evaporation, and freeze-drying to obtain the water-soluble active component of the camphor tree seed kernel. The invention provides a new process for enriching and purifying the active components of the camphor tree seed kernels, the active components of the camphor tree seed kernels are enriched and purified by taking water as an extracting agent and combining with macroporous resin, the operation is simple and convenient, the enrichment efficiency is high, the use amount of organic solvents such as methanol and ethanol is reduced, and the process is safe and environment-friendly. In addition, compared with an ethanol extraction method, the method has the advantages that the camphor tree seed kernel protein is not denatured, and the quality of the camphor tree seed kernel protein is improved; according to the technical scheme, a new solution is provided for comprehensive utilization of camphor tree seed resources.

The invention relates to the technical field of biological nano materials, in particular to a superparamagnetic strong anion exchange magnetic bead for virus enrichment and a preparation method and application thereof. The invention discloses a superparamagnetic strong anion exchange magnetic bead for virus enrichment and a preparation method of the superparamagnetic strong anion exchange magnetic bead. According to the superparamagnetic strong anion exchange magnetic bead, polyglycidyl methacrylate magnetic microspheres serve as raw materials, firstly, a large number of amino groups are introduced through reaction of active epoxy groups and a multi-amino compound, and then abundant quaternary ammonium salt groups are introduced through reaction of amino groups and epoxy quaternary ammonium salt under the alkaline condition. Through two times of epoxy ring-opening reactions, while enough quaternary ammonium salt groups are introduced, a large number of hydrophilic hydroxyl groups are introduced to the surfaces of the magnetic microspheres, so that the hydrophilicity of the anion exchange magnetic beads is improved, and the anion exchange magnetic beads are high in enrichment efficiency and elution efficiency and good in application prospect when being applied to enrichment of viruses.

The invention provides a regulation and control method for rapid screening and enrichment of aerobic granular sludge and a continuous flow reaction system, and relates to the field of sewage treatment. The method is applied to a continuous flow reaction system of the aerobic granular sludge, the continuous flow reaction system of the aerobic granular sludge comprises a sludge scraping device, a sludge settling zone is divided according to preset conditions, and the initial position of the sludge scraping device in the sludge settling zone is determined according to the preset conditions; the mud scraping device is installed at the initial position; regulating and controlling the primary sludge reflux quantity and the secondary sludge reflux quantity according to a preset first sludge concentration range and a preset second sludge concentration range; and when the sludge reflux quantity is changed along with the change of inlet water quality, inlet water quantity or sludge settling performance, and the sludge liquid level of the first-stage sludge reflux sludge bucket or the sludge liquid level of the second-stage sludge reflux sludge bucket exceeds a preset range, the sludge scraping device is adjusted according to the sludge scraping action of the sludge scraping device. The problems that in aerobic granular sludge culture, regulation and control of sedimentation selection pressure are complex, and the granulation process is slow are solved.

The invention discloses a linear mitochondrial separation method, which comprises the following steps: step one, expressing mitochondrial protein peptide with a modified amino acid sequence of NH2-Gly-Gly-Gly-Phe-(D) Arg-Phe-Lys-Phe-(D) Arg-Phe-Lys (Ac)-CON-H2 on a microsphere, so as to obtain a polypeptide modified microsphere with closed GLy, and modifying the mitochondrial protein peptide with the modified amino acid sequence of NH2-Gly-Gly-Gly-Phe-(D) Arg-Phe-Lys-Phe-(D) Arg-Phe-Lys (Ac)-CON-H2; and 2, carrying out mitochondrial extraction on a target tissue by using the GLy-closed polypeptide modified microspheres. The purity of the obtained mitochondria is high, pollution of subcellular organelles and cell debris except the mitochondria is avoided to a certain extent, and the phenomenon that the detection result deviates due to the purity of the mitochondria when the mitochondria with the high purity is detected by a high-sensitivity detection instrument is avoided. And the cost of the mitochondria obtained by the method is controllable, and stable and reliable results can be brought to experiments.

The ultrasonic enrichment and separation system comprises a base, a container is mounted on the base, a lifting support is mounted on the base, a centrifugal tube is mounted at the top end of the lifting support, suspension liquid to be enriched and separated is contained in the centrifugal tube, and the lifting support is used for driving the centrifugal tube to ascend and descend in the container; an ultrasonic transducer is installed on the side face of the container and connected with a signal generator, and the signal generator is connected with a controller. A microscopic imaging module connected with the controller is arranged on one side of the container and used for monitoring the moving track and the moving speed of particles in the centrifugal tube, and the controller adjusts output parameters of the signal generator according to the moving track and the moving speed of the particles. The microimaging module is used for monitoring the utilization speed and the moving track of the particulate matters in the suspension in real time, and the frequency and the power of the signal generator are adjusted according to the utilization speed and the moving track of the particulate matters, so that the enrichment efficiency and the enrichment effect are improved.

The invention relates to the field of medical detection, in particular to a mass spectrometric detection method for enriching serum human insulin by using specific antibody immunomagnetic beads. According to the present invention, the human insulin in the serum is subjected to immune enrichment by using the magnetic beads coated with the insulin specific antibody, and the concentration level of the human insulin in the serum is accurately determined based on the accurate quantification technology of the isotope dilution liquid chromatography-tandem mass spectrometry platform in combination with the bracket method quantification mode. The pretreatment method provided by the invention is simple and convenient to operate, the absolute recovery rate is excellent, the sensitivity requirement is met, and the mass spectrometry method is an isotope diluent liquid chromatography-tandem mass spectrometry method capable of eliminating interference of structural analogues.

The invention provides a method for preparing a lithium iron phosphate material, and belongs to the technical field of lithium ion battery positive electrode materials. According to the method, the Fe element in the industrial iron-containing waste liquid is converted through a microbiological method, then enrichment is conducted through humic acid, the enrichment efficiency is high, and uniformity is good; the microbe-enriched iron complex is used as an iron source and a reducing agent, and the carbon coating uniformity is improved through two-step sintering, so that high magnification and high low-temperature retention rate are realized.

The invention relates to the technical field of glycosylation, and provides a DeepGP glycopeptide enrichment column and a method for efficiently enriching N glycopeptide and O glycopeptide. The preparation method of the DeepGP glycopeptide enrichment column comprises the following steps: uniformly mixing microcrystalline cellulose, cation exchange filler and anion exchange filler, and then packing the mixed filler into a column and compacting to obtain the DeepGP glycopeptide enrichment column. Compared with the enrichment effects of four enrichment columns on HET cell line glycopeptide samples, the DeepGP glycopeptide enrichment column prepared by the invention has the highest parallelism and enrichment efficiency in three times of parallel enrichment. Besides, 245 N glycopeptides can be identified in an IgG standard substance by utilizing a DeepGP column, and 4286 N glycopeptides and 749 O glycopeptides can be simultaneously identified in mouse plasma, so that the method is high in detection precision, high in sensitivity, good in repeatability and very wide in application range.

This invention discloses a deep defluorination agent and its application method, specifically relating to the field of defluorination agents (GMS-FS series products). It includes a valence-state switching active phase, a reaction interface building component, a dynamic electronic regulation component, and a structure adjustment and protection component. The valence-state switching active phase provides a reversible valence-state transition reaction center, achieving specific capture of fluoride ions through an electron trapping effect when interacting with them. The reaction interface building component forms a polar gradient interface layer on the surface of the defluorination agent, guiding fluoride ions to migrate towards the active center and locally enrich them, thereby improving defluorination efficiency. By constructing a rare metal valence-state switching active phase with La2O3 and UO2 as its core, combined with a polar gradient interface guiding layer and a dynamic electronic regulation mechanism, preferential migration and specific capture of fluoride ions are induced in a multi-ion coexistence environment, achieving stable deep defluorination under high salinity and highly competitive ion conditions.

The invention relates to the technical field of bioengineering, in particular to a targeting nanoparticle for treating acute respiratory distress syndrome as well as a preparation method and application of the targeting nanoparticle. Comprising the following steps: (1) combining MP peptide with DSPE-PEG2000-MAL through a maleimide-mercaptan reaction, dialyzing the obtained conjugate, washing, and freeze-drying, so as to obtain a DSPE-PEG2000-MP conjugate; and (2) mixing the conjugate, lipid, a pharmaceutical preparation and a solvent, removing the solvent to obtain a lipid film, hydrating the lipid film, and performing membrane extrusion to obtain the nanoparticles. The invention not only provides a novel efficient and safe ARDS treatment scheme, but also provides an innovative mode for the design of a nano-drug delivery system based on cell mediation. And due to the dual targeting capability, excellent anti-inflammatory efficiency and good biocompatibility, the nano-material has a wide prospect in clinical application.

The invention provides a portable VOCs enrichment and analysis device and portable chromatographic equipment. The portable VOCs enrichment and analysis device comprises a cold trap main body, a cold trap main body, a gas inlet and a gas outlet, an enrichment tube; a heat conduction block; a refrigeration unit; the cold trap body comprises a body part and a cover plate, the upper end of the body part is provided with an opening end, the cover plate is detachably connected to the upper end of the body part, and the body part and the cover plate define a cavity; the heat conduction block comprises two split parts, the two split parts wrap the enrichment pipe in a buckling mode in the vertical direction, each air supply unit comprises a sealing joint, a connecting air column and a connector, the sealing joints are installed on the cold trap body and located at the two ends of the enrichment pipe, the outer ends of the connecting air columns are detachably connected with the connectors, and the connectors are connected with the connecting air columns. The connecting gas column and the connector penetrate through the sealing joint in a sliding manner, and the connecting gas column is detachably connected with the enriching pipe. The refrigeration units form a stepped structure, the refrigeration temperature can reach-40 DEG C, the enrichment efficiency is improved, and the replacement convenience and air tightness of the enrichment pipe are improved through the structural design.

The utility model discloses a new pollution enrichment device, including workstation and installing the rotary base of workstation top, install the material taking subassembly on the rotary seat, the top outer wall of workstation still is equipped with independent processing subassembly, the one side of workstation bottom outer wall is equipped with the link frame, is equipped with the medium cylinder of equidistance distribution on the link frame, the independent processing subassembly includes the installation groove setting in the top outer wall both sides of workstation, and the bottom inner wall of two installation grooves all is connected with the toothed disc through bearing, the bottom outer wall of installation groove still is equipped with the gear group of equidistance distribution, and the linkage cooperation is formed between toothed disc and gear group. The utility model discloses a new pollution enrichment device is equipped with the independent processing subassembly of double position, has the technical effect of improving the new pollution enrichment efficiency.

The invention discloses a paper-based electrospray ionization mass spectrometry ion source and a preparation method and application thereof. The paper-based electrospray ionization mass spectrometry ion source comprises enrichment paper, an eluent and a high-voltage power supply, the enrichment paper is a triangular paper base modified by p-mercaptobenzohydrazide encapsulated nano-silver particles, the eluent is a methanol solution of 1-dodecanethiol, and the high-voltage power supply is direct-current voltage + 3-5kV. The enrichment paper can efficiently enrich aldehyde ketone compounds in gas, p-mercaptobenzoyl hydrazine is selected as an enrichment reagent, the enrichment time is shortened, and the enrichment efficiency is effectively improved; the detection limit of an ion trap mass spectrometer loaded with the paper-based electrospray ionization mass spectrometry ion source is lower than 0.1 ppt, the detection sensitivity and accuracy of the mass spectrometer are improved, and the paper-based electrospray ionization mass spectrometry ion source has performance advantages in qualitative and trace analysis aspects.

This invention relates to a method for enhanced cascade separation and resource utilization of calcium-free roasted chromium slag through reducing alkaline roasting. The specific steps are as follows: Calcium-free roasted chromium slag, a reducing agent, and an alkaline reagent are mixed to obtain a chromium slag mixture; the chromium slag mixture is placed at a first temperature and held for a first time under an inert atmosphere, causing the iron oxides in the chromium slag mixture to be reduced, and the aluminum and chromium oxides to be sodium-treated, while inhibiting the reduction of chromium oxides, resulting in a reduced chromium slag mixture; the reduced chromium slag mixture is filtered to obtain filter residue and an aluminum-enriched filtrate, which can be used for alumina production and the recovery of the alkaline reagent; under an inert atmosphere, the filter residue is placed at a second temperature and held for a second time. Due to the separation of aluminum in the filter residue, metallic iron can be efficiently separated by melting, and chromium can be efficiently enriched in the molten residue. This invention can efficiently achieve the cascade resource utilization of iron, chromium, and aluminum components in calcium-free roasted chromium slag.

This invention belongs to the field of pharmaceutical technology for the treatment and prevention of oral diseases, and particularly relates to a porphyrin and ferrocene derivative, nanoparticles, preparation methods, and applications of synergistic photothermal and acoustic therapy for oral diseases. The porphyrin and ferrocene derivative are formed by organically combining tetraaminophenylporphyrin and diformaldehyde ferrocene through imine dynamic covalent bonds. This invention, through innovative raw material coupling methods and functional integration design, synergistically achieves PTT-SDT dual therapy, providing a new and superior technical solution for precise, efficient, and low-drug-resistance treatment of deep oral bacterial-fungal biofilm infections.

This application discloses a composite catalyst for contact electrocatalytic removal of water pollutants, its preparation method, and its application. This composite catalyst, when applied to contact electrocatalytic removal of water pollutants, can continuously generate charge transfer and form a stable, strong interfacial electric field under mechanical disturbance conditions, efficiently degrading organic pollutants in water. It also exhibits good stability and can be reused multiple times. The composite catalyst comprises a TPB-DVA covalent organic framework material and a fluorocarbon polymer coated on the surface of the TPB-DVA covalent organic framework material. The TPB-DVA covalent organic framework material and the fluorocarbon polymer are interfacially bonded through hydrogen bonds.

The application discloses a Bifidobacterium lactis specific protein antibody, an antibody conjugated magnetic bead and application. The antibody is a monoclonal antibody BL1, comprising a heavy chain variable region and a light chain variable region, and the amino acid sequences of the heavy chain variable region and the light chain variable region are shown in SEQ ID NO: 3 and 4 respectively. The antibody conjugated magnetic bead is coupled by the monoclonal antibody BL1 and activated carboxyl magnetic beads. The antibody conjugated magnetic bead can specifically enrich Bifidobacterium lactis, has high enrichment efficiency, simple enrichment operation, and can be applied to identification of metabolic products of Bifidobacterium lactis, and can be used for qualitative and quantitative analysis.

The invention discloses a preparation method of a bionic membrane coated ferrous sulfide nano material based on neutral grains, which comprises the following steps: dissolving bovine serum albumin in deionized water, adding a ferrous chloride tetrahydrate solution and a sodium sulfide solution to obtain a mixed solution, and reacting to obtain an albumin embedded amorphous ferrous sulfide nano material; mixing a neutrophile granulocyte membrane solution, a lipidosome solution and the albumin chimeric amorphous ferrous sulfide nano-material, and performing ultrasonic treatment to obtain the biomimetic membrane coated ferrous sulfide nano-material. The invention also discloses the biomimetic membrane coated ferrous sulfide nano-material obtained by the preparation method and application of the biomimetic membrane coated ferrous sulfide nano-material in preparation of tumor treatment drugs. The biomimetic membrane coated ferrous sulfide nano-material prepared by the invention can realize tumor specificity trend, effectively improves enrichment of the ferrous sulfide nano-material at a tumor site so as to realize efficient tumor specificity killing, and has good biological safety to normal tissues.

The invention relates to a method for enriching and recovering rhodium from organic waste liquid containing rhodium acetylacetonate, and belongs to the technical field of precious metal secondary resource recovery. The method comprises the following steps: carrying out reduced pressure evaporation on an organic waste liquid containing rhodium acetylacetonate, separating, condensing and recovering low-boiling-point organic components to obtain a distillation residual liquid containing rhodium acetylacetonate; adding a water phase complex breaking and phase inversion system into the distillation residual liquid, stirring and reacting to break complex of rhodium acetylacetonate and promote rhodium to transfer into a water phase, and separating an organic phase from the water phase to obtain a rhodium-containing water phase; adjusting the rhodium-containing water phase by using an alkaline solution under a stirring condition, stably controlling the pH value to be 6-9, simultaneously dropwise adding a sulfuration precipitant solution, reacting to generate rhodium-containing sulfide precipitates, carrying out heat preservation and aging treatment for 0.5-4 hours, carrying out solid-liquid separation to obtain rhodium enriched products, and sequentially washing and drying the rhodium enriched products to obtain the rhodium enriched products. The method is simple in process and mild in condition, and efficient phase inversion and enrichment recovery of rhodium in the rhodium acetylacetonate-containing organic waste liquid can be realized.

The invention relates to the technical field of detection equipment, in particular to equipment for detecting genes of odor substances in water on site. The device comprises a sampling system and a suction filtration system, the sampling system comprises a mechanical arm, the suction filtration system comprises a suction filtration platform, the mechanical arm is installed on the suction filtration platform, a sampling device is installed at the bottom of the mechanical arm, at least four first through holes are symmetrically formed in the suction filtration platform, and suction filtration devices are installed in the first through holes; according to the device disclosed by the invention, accurate collection of samples and uploading of sample information are completed through the sampling system and the suction filtration system of the device, so that information such as sampling positions is more accurate, the labor cost is greatly saved, and the timeliness of the samples is improved; through the mechanical arm of the fluorescent quantitative PCR system of the device, accurate preparation of the quantitative PCR system is realized, and proper primers are selected, so that manual errors are reduced, and powerful guarantee is provided for in-situ recognition of olfactory genes and early warning of odor risks.

The invention discloses a high-affinity nano antibody for resisting a nervous centralis specific protein S100beta or an antigen binding fragment of the high-affinity nano antibody and application of the high-affinity nano antibody, and belongs to the technical field of antibodies. The amino acid sequence of the nano antibody is as shown in SEQ ID NO: 2, and the nucleotide sequence of the nano antibody is as shown in SEQ ID NO: 1. The invention provides a high-affinity antibody with EC50 reaching 4.0 nM and a recombinant expression scheme capable of realizing large-scale production, and provides a high-affinity and low-cost S100beta nano antibody by reasonably designing a four-round screening strategy.

The utility model provides a protein enrichment device which comprises a heat preservation shell and a test tube rack, the test tube rack is arranged in the heat preservation shell, and a gap is formed between the outer wall of the test tube rack and the inner wall of the heat preservation shell; a plurality of test tube channels for placing test tubes are transversely arranged in the test tube rack; and a test tube rack body is arranged among the test tube channels and is made of a heat-conducting material. According to the device provided by the utility model, the separation between the target protein and the target protein antibody beads caused by liquid dripping is reduced, so that the enrichment efficiency of the protein is effectively improved.

The present invention discloses a synchronous denitrification and dephosphorization microbial battery sewage treatment system and method, the system comprises a double-chamber microbial battery, a single-chamber microbial battery, a first crystallization tank and a second crystallization tank; the double-chamber microbial battery comprises a double-chamber battery chamber, a double-chamber battery resistor, a double-chamber battery anode, a double-chamber battery cathode and a double-chamber battery cation exchange membrane, the single-chamber microbial battery comprises a single-chamber battery chamber, a single-chamber battery resistor, a single-chamber battery anode and a single-chamber battery cathode; the second crystallization tank, the double-chamber battery chamber and the single-chamber battery chamber are all connected to the first crystallization tank. The system can be used for low C / N sewage treatment, can remove ammonia nitrogen and phosphorus at the same time, does not need to carry out additional nitrification and denitrification processes, aeration and aeration oxygen supply and other links, the treatment process can simultaneously obtain high-purity magnesium ammonium phosphate, diammonium hydrogen phosphate and diammonium dihydrogen phosphate and other products that can be directly used as fertilizers, and has the characteristics of good sewage treatment effect and high recycling efficiency.

The invention provides a nano preparation for preparing a medicine for inhibiting prostatic cancer and a preparation method of the nano preparation, and relates to the technical field of nano medicinal preparations. The nano preparation comprises the following components in parts by mass: traditional Chinese medicine components and a nano carrier, the traditional Chinese medicine component comprises 15-45 parts of a Chinese yam extract, 5-15 parts of a madder extract, 8-20 parts of cuttlebone superfine powder, 10-25 parts of dragon bone superfine powder and 10-25 parts of oyster superfine powder; the nano-carrier comprises 5-20 parts of phospholipid, 2-10 parts of cholesterol, 1-5 parts of fatty acid, 3-15 parts of polyethylene glycol and 1-5 parts of a surfactant. The average particle size of the nano preparation is 80-150 nm, the polydispersity index PDI is smaller than or equal to 0.30, and the encapsulation efficiency is larger than or equal to 75%. According to the invention, active ingredients of traditional Chinese medicines are combined with a nanotechnology, so that the bioavailability and the tumor targeting property are remarkably improved, the pH-responsive release characteristic is achieved, a remarkable inhibition effect on various prostate cancer cell lines is shown, tumor growth can be effectively inhibited, metastasis can be reduced, and a good application prospect is achieved.

The invention relates to development and application methods of a novel enriched cross-linking agent based on sulfur-fluorine exchange reaction (SuFEx), and belongs to the technical field of organic synthesis. According to the invention, a series of cross-linking agents with phenylmethylsulfonyl fluoride at a single end or double ends are synthesized on the basis of an existing trimethyl piperidyl bis-succinimide ester cross-linking agent and based on a SuFEx reaction. The cross-linking agent provided by the invention has the characteristics of enrichment, adjustable arm length, multiple reaction sites, isotope quantification and the like, not only improves the cross-linking depth and coverage, but also can realize relative quantification of protein. And an important technical support is provided for in-situ protein analysis based on a chemical crosslinking strategy.

This invention provides the application of a siRNA composition targeting LOC157273 in the preparation of drugs for treating non-alcoholic steatohepatitis (NASH) and liver fibrosis, belonging to the field of biomedical technology. The siRNA of this invention directly corrects the hyperlipidemic hepatic lipid synthesis, insulin resistance, and inflammatory activation caused by abnormal expression of PPP1R3B by specifically silencing the key fibrosis driver gene LOC157273. This fundamentally blocks the activation of hepatic stellate cells and excessive extracellular matrix deposition, achieving precise reversal of the fibrosis process and blocking the occurrence of NASH. The siRNA delivery system is optimized through liver-targeted modification, improving liver enrichment efficiency and reducing off-target effects caused by systemic distribution. It exhibits strong targeting, directly acting on upstream regulatory genes of fibrosis and NASH. Simultaneously, it silences multiple synergistic pathogenic gene sites, enhancing therapeutic efficacy and providing a highly efficient and safe novel intervention for liver fibrosis and NASH.

The invention relates to a nano drug delivery system for targeted removal of liver aging cells and regulation and control of oxidative stress as well as a preparation method and application of the nano drug delivery system. The nano drug delivery system comprises cerium-doped zinc-based metal organic framework nanoparticles, a drug loaded in a hollow mesoporous structure of the nanoparticles, and a bone marrow mesenchymal stem cell membrane modified on the surfaces of the nanoparticles. According to the invention, a nano drug delivery system for breaking an'aging-inflammation 'cycle is constructed on the basis of an interaction relationship between aging cells and the non-alcoholic fatty liver disease, and the enrichment efficiency and the residence time of the nano drug delivery system at a non-alcoholic steatohepatitis disease part can be improved; the hollow mesoporous metal organic framework nano particles with the effects of efficiently removing active oxygen and being high in drug loading rate are used for loading drugs, the targeting performance on liver senescent cells, the synergistic effect and safety of removing senescent cells and adjusting the inflammatory environment are improved from the two links of drug delivery and release, and liver lipid metabolism and the inflammatory environment are improved.