Cancer monitoring-oriented biological coating for specific whole blood capture of circulating tumor cells and preparation method of biological coating

Abstract

The invention provides a cancer monitoring-oriented biological coating for the specific whole blood capture of circulating tumor cells and a preparation method of the biological coating and belongs tothe field of biotechnology and clinics. According to the coating, polyglycidyl ether (PG) or polyethylene glycol (PEG) is adopted, the adhesion bionic chemical modification of mussels is used in combination, and therefore, the specific capture of the circulating tumor cells can be performed. According to the biological coating, and the preparation method thereof, the surface of a substrate is modified with a functionalized polymer; the adhesion of non-specific cells to other blood components is prevented; a specific antibody is fixed on the surface of the polymer; a to-be-detected sample (blood) is dropwise added to the surface of the coating; the synergistic effect of specific adsorption and non-specific adhesion resistance is achieved through the synergistic effect of the specific antibody for identifying the circulating tumor cells and the non-specific adhesion resistance polymer, so that the circulating tumor cells in the to-be-detected sample can be efficiently and accurately captured. With the method provided by the invention adopted, the circulating tumor cells can be efficiently captured. The method has the advantages of low cost, simple operation and extremely high sensitivity, and can be used for clinical detection.

Description

technical field

[0001] The invention belongs to the field of functional material technology and biomedical materials, and in particular relates to a specific biological coating for enrichment and detection of circulating tumor cells and a preparation method thereof. Background technique

[0002] Malignant tumors have a tendency to metastasize and recur, and are the main cause of death in cancer patients. Tumor metastasis is when tumor cells break away from the primary lesion, invade the extracellular matrix, invade the circulatory system, effectively evade the body's immune clearance system, and become circulating tumor cells (CTCs). Although the number of CTCs in blood is very small (several to hundreds per milliliter) mixed with a large number of blood cells (10 9 per milliliter), but it is of great significance in examining cancer metastasis and monitoring the effect of treatment. That is to say, CTCs can not only replace biopsy to find cancer metastasis, but also the n...

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