301 results about "HeLa" patented technology

The invention relates to the field of natural products, in particular to a dimer compound of guaiane type sesquiterpenes and uric acid as well as an extraction and separation method and application of the dimer compound. According to the invention, a new dimer compound of guaiane type sesquiterpene and uric acid is extracted and separated from carpesium abrotanoides in Guizhou, and it is found that the dimer compound has relatively strong anti-tumor activity. The compound has strong inhibitory activity on breast cancer, prostate cancer, nasopharynx cancer, liver cancer, lung cancer, leukemia, pancreatic cancer, glioma, osteosarcoma, skin cancer, cervical cancer, ovarian cancer, kidney cancer and esophageal cancer, and provides an optional scheme for broad-spectrum anti-cancer drugs. Moreover, in human prostate cancer cells PC3, human cervical cancer cells Hela, human liver cancer cells HepG2 and human glioma cells U87 which are resistant to paclitaxel, the compound also shows strong anti-cancer activity, which indicates that the compound has the potential of treating patients resistant to paclitaxel.

The invention relates to the technical field of biological medicine, and discloses an aromatic polyketone compound as well as a preparation method and application thereof. According to the aromatic polyketone compound, a compound I and a compound II are separated from an ethyl acetate extract of rice fermentation liquor of Crystalomyces hyricus, and the compound I and the compound II are respectively named as Crystaloketide A and Crystalomycin D. In-vitro anti-cancer activity tests are carried out on the Crystaloketide A and the Crystalomycin D. According to the aromatic polyketone compound disclosed by the invention, the anti-cancer activity of the Crystaloketide A and the Crystalomycin D. Results show that the compound I and the compound II have the effects of inhibiting human cervical cancer cells, human liver cancer cells, human nerve cancer cells and human pancreatic cancer cells respectively, so that the compound I and the compound II can be further prompted to be used for preparing anti-cancer drugs.

The invention belongs to the field of plant endophytic fungus secondary metabolites, particularly relates to a secondary metabolite of fennel endophytic fungus fusarium oxysporum as well as a preparation method and application thereof, and provides a fennel endophytic fungus secondary metabolite with structures as shown in compounds 1-8. Wherein the compound 2 and the compound 7 have inhibitory activity on staphylococcus aureus, escherichia coli, bacillus cereus, bacillus subtilis, candida albicans and shigella, and have a certain inhibitory effect on cervical cancer Hela cell strains.

The invention belongs to the technical field of medicinal chemistry, and particularly relates to a 3-sulfamoyl benzamide compound as well as a preparation method and application thereof. The 3-sulfamoyl benzamide compound has a structure as shown in a formula I. In the formula I, X is chlorine or trifluoromethyl; r1 and R2 are independently C1-4 alkyl or R1, R2 and N atoms connected with R1 and R2 jointly form a heterocyclic group, and the heterocyclic group is a substituted or unsubstituted five-membered or six-membered heterocyclic group; and when the heterocyclic group is a substituted heterocyclic group, the substituent group in the substituted heterocyclic group is one or more of C1-4 alkyl, phenyl and acetyl. The 3-sulfamoyl benzamide compound disclosed by the invention has a remarkable proliferation inhibition effect on HCT116 colon cancer cells, HeLa human cervical cancer cells, MDA-MB-231 human breast cancer cells and A375 human malignant melanoma cells.

The invention discloses a single cell activity evaluation method based on cell nucleus geometric parameters and a Bayesian framework, which comprises the following steps: establishing a HeLa cell activity gradient model through adriamycin induction, carrying out cell nucleus segmentation by adopting Cell pose 3.0 and extracting 26 geometric features, screening a high-confidence sample in combination with a Bayesian deep learning framework, and evaluating the activity of a single cell according to the high-confidence sample. And constructing a multi-modal feature fusion model to integrate geometric features and deep semantic features, and finally realizing unmarked and high-precision single cell activity evaluation. The method overcomes the limitation that a traditional technology depends on biochemical markers, has the advantages of being easy and convenient to operate, high in flux and high in interpretability, and provides an innovative technical means for tumor liquid biopsy and precise medical treatment.

The invention discloses a preparation and purification method and application of a quinoline derivative with biological activity, and belongs to the technical field of medicine synthesis. According to the key points of the technical scheme, the quinoline derivative molecule has a structure # imgabs0 #, wherein X is NH or empty (benzene rings are directly connected); r is an aromatic ring derivative or alkyl or other groups. The invention designs and synthesizes a quinoline derivative with a novel structure, and the compound has a relieving effect on LPS-induced microglial cell inflammation and has a certain inhibition effect on cervical cancer cells.

The invention provides a monoclonal antibody for detecting E6 protein in cervical cancer cell nucleus and application of the monoclonal antibody. The invention provides the monoclonal antibody which is high in specificity, strong in affinity, stable in expression, good in repeatability and capable of specifically recognizing the HPV 16E6 protein in a cell nucleus, especially the HPV 16E6 protein, and has the potential of being used for preparing medicines for preventing and treating cervical cancer.

The present invention provides a biomimetic MOF nanoplatform that can dual-target cervical cancer tumor cells and cancer-associated fibroblasts and co-deliver FAK inhibitors and bismuth, and its preparation method and application. The biomimetic MOF nanoplatform includes a MOF material loaded with FAK inhibitors (IN10018) and bismuth (Bi), and a hybrid membrane coated on the outside of the MOF material loaded with FAK inhibitors and bismuth. The hybrid membrane is obtained by physical extrusion after mixing cervical cancer tumor cell (HeLa and SiHa) membranes and cancer-associated fibroblast (CAFs) membranes. In an acidic environment, the biomimetic MOF nanoplatform of the present invention disintegrates ZIF-8 nanoparticles loaded with IN10018 and Bi, releases IN10018, reduces CAFs infiltration in the tumor, and at the same time, the addition of Bi improves radiation absorption efficiency, further enhancing the sensitivity of the tumor to radiotherapy through a dual-targeting mechanism.

The invention discloses a probe set, a kit and a nucleic acid signal amplification in-situ hybridization detection method, and relates to the technical field of molecular biology, the probe set comprises: a labeled extension probe, the sequence of which is as shown in SEQ ID No.9-24; and the sequence of the closed probe is as shown in SEQ ID No. 25-27. The kit comprises the probe set, a signal amplification precursor, a signal amplification body and an alkaline phosphatase labeled probe. According to the present invention, the partial probe sequence introduces the non-natural bases, and can be used for detecting the HPV18 infected cervical cancer Hela cells, the experiment results show that the introduction of the non-natural bases does not affect the normal positive value, the matching degree between the non-natural bases is more specific than the matching of the natural bases, and the detection sensitivity is improved.

The invention discloses a preparation method and application of an electrochemical biosensor for dynamically monitoring Cyt c in tumor cells based on fast scanning voltammetry. The preparation method is characterized by comprising the following steps: 1) preparing a PtNE nano electrode; (2) preparing the PtNE / Apt nano electrochemical biosensor; and (3) accurately puncturing the PtNE / Apt electrode into cytoplasm of a single Hela cell through a three-dimensional micro-control system, so that Apt and intracellular Cyt c are specifically combined and subjected to conformational change, and 3 '-end modified Fc is close to the surface of the electrode, thereby generating a remarkable electrochemical signal. The change of Fc redox peak current is dynamically monitored through fast scanning voltammetry, the quantitative relation between the Cyt c concentration and the peak current is established, and the relation between the Cyt c concentration and the cell activity is monitored according to the quantitative relation. The device has the advantages of high sensitivity, excellent anti-interference capability and remarkable signal amplification effect.

The invention relates to a 5, 9-di-tert-butyl naphtho-indolizine phenothiazine compound as well as a preparation method and medical application thereof. The compound is efficiently synthesized through Ullmann coupling and palladium-catalyzed intramolecular arylation reaction. In-vitro anti-tumor activity research shows that the compound has remarkable selective inhibitory activity on various human tumor cell lines, and particularly, the inhibitory effect on non-small cell lung cancer A549 cells (IC50 = 0.21 mu M) is improved by two orders of magnitude compared with that of cis-platinum; iC50 (half maximal inhibitory concentration) of other cell lines are as follows: 1.26 mu M of prostate cancer PC-3 cells, 6.78 mu M of liver cancer HepG2 cells, 7.99 mu M of cervical cancer Hela cells and 25.46 mu M of breast cancer MCF-7 cells. Preliminary toxicity experiments prove that the compound has the characteristic of low cytotoxicity. The compound can be used as a novel high-efficiency low-toxicity anti-tumor lead compound, and provides an important structural basis for the development of anti-cancer drugs.

The invention provides a transfection reagent based on blank lipid nanoparticles as well as a preparation method and application of the transfection reagent, and belongs to the technical field of biological medicines. The transfection reagent based on the blank lipid nanoparticles comprises the following components: (1) the blank lipid nanoparticles; (2) a biologically active ingredient; the blank lipid nanoparticles comprise ionizable lipid, phospholipid, cholesterol and polyethylene glycol conjugated lipid. The preparation process of the blank lipid nanoparticles and the transfection reagent is simple, and the preparation can be completed without the help of equipment. The transfection reagent based on the blank lipid nanoparticles is prepared from the blank lipid nanoparticles and nucleic acid, the dosage can be flexibly adjusted according to the requirements of a user, the transfection effect is stable, various cells (such as 293T, Hela, HepG2, TWO3 and MCF7) can be transfected, the cytotoxicity is low, and the transfection effect is good.

The invention relates to the technical field of separation and purification of radix arnebiae seu lithospermi, in particular to a naphthoquinone compound in radix arnebiae seu lithospermi, a preparation method of the naphthoquinone compound and application of the naphthoquinone compound in preparation of drugs for treating cervical carcinoma, the naphthoquinone compound in radix arnebiae seu lithospermi is chemically named as (Z)-2-ethoxy-5, 8-dihydroxy-3-(4-methyl-1, 3-pentadiene-1-yl) naphthalene-1, 4-diketone, and the structural formula of the naphthoquinone compound is shown in the description. The naphthoquinone compound in the lithospermum is disclosed for the first time, in-vitro anti-tumor pharmacodynamic experiments are carried out on the naphthoquinone compound in the lithospermum, and the experiments show that the naphthoquinone compound in the lithospermum has a certain inhibition effect on human Hela cells; therefore, the naphthoquinone compound in the radix arnebiae seu lithospermi can be applied to preparation of drugs for preventing / treating cervical cancer.

The invention discloses application of a tripterygium wilfordii exosome in preparation of an anti-cervical cancer medicine and a tumor angiogenesis inhibiting medicine, and relates to the field of biological medicine. The medicinal plant exosome derived from tripterygium wilfordii is successfully separated, and it is proved that the medicinal plant exosome can regulate related pathways through delivery of functional nucleic acid molecules to inhibit proliferation and migration of cervical cancer cells and promote active oxygen related apoptosis so as to be used for tumor treatment. The tripterygium wilfordii exosome can further act on vascular endothelial cells in a tumor microenvironment, the migration function of Hela cells is inhibited, and then the effect of resisting cell proliferation in the tumor microenvironment is achieved. The tripterygium wilfordii exosome has the advantages of being simple in preparation method, remarkable in tumor growth resistance and tumor cell migration inhibition effect, good in biocompatibility, high in delivery efficiency and the like, and can be used as a novel nano-drug for tumor treatment.

The invention relates to the technical field of medical information processing, in particular to a cervical cancer cell early screening and intelligent evaluation system based on multi-modal data, which comprises a data acquisition module, a form detection module and a screening early warning module, the data acquisition module is used for acquiring multi-modal data of a current detection object in a plurality of inspection processes; the morphology detection module is used for calculating a cellular morphology score of a current detection object in any target inspection process; the screening early warning module is used for determining similar detection objects of the current detection object, and correcting differences between the current detection object and the similar detection objects in the aspects of cellular morphology scores and lesion detection indexes according to the approximation degree between different types of lesion detection indexes of the current detection object; and obtaining the screening early warning coefficient of the current detection object by using the relative change condition of the correction result. According to the invention, the early screening and evaluation effects on cervical cancer cells are improved.

The present application relates to a kind of five-ring thiazole indole ketone piperazine amide derivatives and purposes thereof.The derivatives take ethyl cyanoacetate as starting material, generate hydroxylamine compound (A) under the action of sodium nitrite and phosphoric acid;Obtain 2-amino ethyl cyanoacetate (B) by reduction, in turn with acetic anhydride, lawson reagent is reacted, and 5-amino-4-carboxylate thiazole compound (D) is obtained;After bromination by NBS, under the catalysis of phosphorus oxychloride, react with tetrahydropyridine indole ketone, generate 2-bromo-6,7-dihydrothiazolo [5'',4'':4',5'] pyrimido [1',2':1,2] pyrindo [3,4-b] indole-4 (12H) -ketone (F), again under alkaline condition, first with Boc piperazine, then by Boc treatment and obtain compound (H);Finally, react with acyl chloride, and 28 different substitution structures of five-ring thiazole indole ketone piperazine amide compound (I1-I28) are synthesized.The 28 compounds are tested on three kinds of cancer cells, and the results show that: 28 compounds have significant inhibitory activity on Hela cervical cancer cells, HT-29 human colon cancer cells and HGC-27 human gastric cancer cells.

This invention belongs to the field of organic synthesis and relates to a nitrogen-containing [3,2-b]indole derivative, its preparation method, and its application. The nitrogen-containing [3,2-b]indole derivative is a compound, optical isomer, or pharmaceutically acceptable salt thereof, as shown in the following structure: ; wherein R1, R2, R3, and R4 are independently selected from hydrogen atoms, halogens, cyano groups, C1-C8 alkyl groups, and C1-C8 alkoxy groups; R5, R6, R7, and R8 are independently selected from hydrogen atoms, halogens, and C1-C8 alkyl groups; R9 is selected from C1-C8 alkyl groups; when R1, R2, R3, R4, R5, R6, R7, and R8 are all hydrogen atoms, R9 is selected from C1-C2 alkyl groups. The compound exhibits antitumor activity, particularly against HeLa cervical cancer, showing a significant inhibitory effect on its proliferation.

The invention belongs to the field of organic synthesis and medicinal chemistry, and particularly provides an efficient synthesis method of an N, N-diphenyl aniline compound based on a naphtho-indolizino-phenothiazine skeleton and anti-tumor application of the N, N-diphenyl aniline compound based on the naphtho-indolizino-phenothiazine skeleton. Through Suzuki reaction, functional coupling based on a naphtho-indolizino-phenothiazine skeleton and an N, N-diphenyl aniline group is realized for the first time. In-vitro anti-tumor evaluation shows that the compound has remarkable inhibitory activity on various human tumor cells, especially has the strongest effect on breast cancer MCF-7 cells (IC50 = 0.15 mu M), and the activity is improved by 266 times compared with that of cis-platinum; prostate cancer PC-3, liver cancer HepG2, lung adenocarcinoma A549 and cervical cancer Hela cells are also remarkably inhibited. The toxicity to normal umbilical vein endothelial cells (HUVEC) is far lower than that of tumor cells, and the selectivity is excellent. The research provides a lead compound with great potential for developing high-efficiency and low-toxicity targeted anti-tumor drugs.

The invention discloses a preparation method and application of a tridentate metal iridium complex, the preparation method of the tridentate metal iridium complex comprises the following steps: step 1, substituted 1, 3-benzene diformate and 2-aminothiophenol are subjected to cyclization reaction to obtain a first intermediate; 2, reacting iridium trichloride hydrate with the first intermediate obtained in the step 1 to obtain a second intermediate; and step 3, reacting an LX ligand with the second intermediate obtained in the step 2, and then using silver trifluoromethanesulfonate to obtain a target product. The tridentate clamp type metal iridium complex disclosed by the invention is composed of a substituted-1, 3-bis (benzothiazolyl) benzene main ligand, a 2, 2-dipyridyl or phenanthroline NN ligand and balanced chloride ions. The complex is simple in synthesis method and has high cytotoxicity to cervical cancer cells, colon cancer cells and lung cancer cells, and the in-vitro cell toxicity of the complex is obviously superior to that of clinically applied anti-tumor drug cis-platinum, so that the complex has a good application prospect in the field of metal anti-cancer drugs.

The invention provides a 1, 3, 4-oxadiazino tetrahydroisoquinoline compound as well as a preparation method and application thereof, and belongs to the technical field of heterocyclic compounds for medicines. The method comprises the following steps: by taking an azomethine imine substrate and a styrene oxide substrate as raw materials and cobalt perchlorate hexahydrate as a catalyst, adding the raw materials and the catalyst into a reaction solvent, reacting overnight at normal temperature, and performing column separation to obtain the 1, 3, 4-oxadiazino tetrahydroisoquinoline compound. According to the application, the reaction condition is mild, the selectivity is high, excellent cytotoxicity is shown on HeLa cervical cancer cells and A549 lung cancer cells, the half death inhibition ratio (IC50) value on tumor cells reaches the nanomole level, and the application has a good anti-cancer drug application prospect.

The invention relates to an application of Edwardsiella fish-sourced protein EseO or a coding gene thereof in preparation of medicines for treating and / or preventing obesity. The edwardsiella fish source protein EseO screened by the invention can inhibit lipid metabolism of cells HeLa and mice C57 / BL6J, which proves that the edwardsiella fish source protein EseO has an obvious fat reducing effect and can be applied to feed additives, medical products and the like in the aquaculture industry related to weight loss.

The invention discloses a small molecule compound, a water-soluble fluorescent probe as well as a preparation method and application of the water-soluble fluorescent probe, and belongs to the technical field of medicines. According to the invention, a small molecule compound NQL containing a naphthalimide fluorophore is synthesized, and then a water-soluble fluorescent probe CS-NQL is prepared by forming Schiff base and coupling the Schiff base with chitosan oligosaccharide. The CS-NQL has environmental viscosity responsiveness and lysosome targeting specificity, so that tumor and inflammatory cell imaging can be carried out in a visible light range. The CS-NQL and DNA are subjected to electrostatic self-assembly to form nanoparticles CS-NQL coated DNA NPs, and the nanoparticles CS-NQL coated DNA NPs can generate active oxygen under an anoxic condition; under the irradiation of 660 nm laser, HeLa cell apoptosis can be induced, and an antibacterial effect is shown. An in-vivo experiment shows that CS-NQL-coated DNA NPs shows a strong anti-tumor immune response under the irradiation of 660 nm laser, so that the tumor is completely eradicated.

The application discloses a dihydroquinazolinone-containing N-hydroxy amide compound and a preparation method and application thereof, relates to the technical field of medicinal chemistry, and utilizes the splicing principle to design and synthesize dihydroquinazolinone-containing N-hydroxy amide compounds with novel structures, and the compounds are tested in terms of HDAC6 protein affinity and anti-proliferation activity of cervical cancer cell Siha, and the results show that the compounds E1-E5 have better affinity to the HDAC6 protein, the IC 50 values are 4.1-85.4 nM, wherein the affinity of the compound E4 to the HDAC6 protein is higher than that of a positive control SAHA; and the compounds E1-E5 can better inhibit the proliferation of the cervical cancer cell Siha, the IC 50 values are 2.4-9.3 muM, wherein the activity of the compound E4 is the strongest.

The invention discloses a supramolecular self-assembled fiber as well as a preparation method and application thereof in cells. The supramolecular self-assembled fiber is obtained by mixing a cis, cis-cyclohexane-1, 3, 5-triformyl hydrazine mother solution, a p-formylphenylboronic acid mother solution and a 5-(2-aminophenyl) tetrazole mother solution in a phosphate buffer solution; the application of the supramolecular self-assembled fiber in cells comprises the following steps: inoculating a pore plate with Haila cells, gently and horizontally shaking, culturing, washing, incubating in a complete DMEM (Dulbecco Modified Eagle Medium) solution of 5-(2-aminophenyl) tetrazole, and washing to obtain pretreated cells; and further incubating and washing the pretreated cells in a complete DMEM (dulbecco's modified eagle medium) solution containing cis, cis-cyclohexane-1, 3, 5-triformyl hydrazine and p-formylphenylboronic acid, so as to obtain the cells enriched with the boron element. Compared with a small molecular boron carrying agent, the supramolecular self-assembled fiber prepared by the invention realizes effective boron element enrichment, and has the advantages of high affinity to tumor cells, good stability and low biotoxicity.

The present invention discloses a biosensor for detecting telomerase activity, its preparation method and application, which relates to the technical field of biosensors. The preparation method includes the following steps: after the indium tin oxide electrode is cleaned, a Zn-MOFs@Au NPs composite is dropped onto its surface to obtain a Zn-MOFs@Au NPs / ITO electrode; the Zn-MOFs@Au NPs / ITO electrode is immersed in an LH:P double-stranded solution for an incubation reaction to obtain the biosensor; the Zn-MOFs@Au NPs composite is obtained by mixing and reacting gold nanoparticles and zinc-based metal-organic framework materials. The biosensor prepared by the present invention has advantages such as high accuracy, strong sensitivity and good selectivity, and can be applied to the accurate detection of telomerase in cervical cancer cells.

The invention belongs to the technical field of biomedicine, and particularly relates to application of SOX4 in preparation of a preparation for improving sensitivity of cervical cancer to chemotherapeutic drugs, and the amino acid sequence of the SOX4 is as shown in SEQ ID NO.1. An SOX4 knocked-down stable transfection cell line is constructed in a Hela cell, then carboplatin and oxaliplatin are respectively added into the SOX4 knocked-down Hela cell, and the IC50 of the cell to the carboplatin and the oxaliplatin is obviously reduced, which indicates that the sensitivity of the Hela cell to the carboplatin and the oxaliplatin is enhanced by SOX4 knock-down. By reducing the expression of SOX4, the sensitivity of Hela cells to chemotherapeutic drugs such as carboplatin and oxaliplatin is enhanced, so that the treatment effect of the chemotherapeutic drugs such as carboplatin and oxaliplatin is effectively improved. Results of the invention prove that inhibition of SOX4 is beneficial to recovery of chemosensitivity of cervical cancer cells to drugs such as carboplatin and oxaliplatin, and a new treatment strategy is provided for treatment of drug-resistant cervical cancer.

The invention discloses a multi-target carboplatin-platinum (IV) complex as well as a preparation method and application thereof, and belongs to the field of medicinal chemistry. The complex has two different bioactive ligands in the axial direction, carboplatin is used as a raw material, carboplatin is oxidized into oxyplatin in hydrogen peroxide, and then the oxyplatin, axial ligand NHS ester and anhydride are subjected to continuous two-time esterification to obtain a'multi-target 'platinum (IV) anticancer prodrug candidate. 1 / 1 NHS ester and platinum oxide are exchanged in DMSO in the first esterification, and 10 / 1 anhydride and a monosubstituted complex are exchanged in DMF in the second esterification; the reaction raw materials are easy to obtain, the synthesis steps are simple, and the reaction conditions are mild. The multi-target carboplatin complex shows good physiological activity on HeLa, C33A, SiHa and other cervical cancer cells, and is expected to be applied to potential anti-cancer drugs.

The invention relates to a sesquiterpenoid glycoside compound in Atractylodes lancea, and a preparation method and application thereof. The invention belongs to the technical field of medicines, and relates to a method for extracting and separating a sesquiterpenoid glycoside compound (1S, 5S, 7R, 10S)-10-hydroxy-deoxy split-ring atractylodes macrocephala delta-lactone-11-O-beta-D-glucopyranoside from rhizome of Atractylodes lancea by using normal phase silica gel, reverse phase silica gel, preparative high performance liquid chromatography and other technologies. In-vitro anti-tumor activity screening experiments show that the compound has good anti-tumor activity, including obvious inhibition effects on human lung cancer cells A549, human cervical cancer cells Hela and human liver cancer cells HepG-2. Therefore, the sesquiterpenoid glycoside compound has a prospect of being developed into antitumor drugs.

The invention belongs to the technical field of biology. The invention relates to the field of medicine, in particular to application of grifola frondosa fermentation liquor in preparation of medicine for treating cervicitis with high-risk human papilloma virus, gynecological gel for treating high-risk human papilloma virus infection and a preparation method and application of the gynecological gel. The grifola frondosa fermentation liquor is obtained by taking grifola frondosa as a fermentation strain and adding a phellinus igniarius extract into a fermentation substrate for liquid submerged fermentation. Tests prove that the grifola frondosa fermentation liquor can obviously inhibit the expression of E6mRNA in SiHa cells and HeLa cells; in a clinical test, the grifola frondosa fermentation liquor combined with the recombinant human interferon a2b has a remarkable curative effect on treating cervicitis with HPV infection, the HPV negative conversion rate can be effectively increased, the inflammatory factor secretion level can be reduced, the ecological restoration balance of the vagina microenvironment can be promoted, and yang recovery is not easy after treatment.

This invention provides a 2-sulfonylpyrimidine-4-amide compound and its uses, belonging to the field of antitumor drug technology. The compound of this invention exhibits excellent antitumor activity targeting the colchicine site of tubulin, demonstrating antiproliferative activity against tumor cells such as HeLa, HepG2, H1299, and MCF-7 at the micromolar level, and significantly inhibiting colony formation in a dose-dependent manner. Furthermore, the compound inhibits tubulin polymerization and disrupts the microtubule network of H1299 cells in vitro, inducing cell cycle arrest in the G2 / M phase and apoptosis. More importantly, the compound can inhibit angiogenesis in HUVECs in vitro. The compound of this invention has great potential in the preparation of microtubule destabilizing agents targeting the colchicine site.