44 results about "HeLa" patented technology

The present application relates to a kind of five-ring thiazole indole ketone piperazine amide derivatives and purposes thereof.The derivatives take ethyl cyanoacetate as starting material, generate hydroxylamine compound (A) under the action of sodium nitrite and phosphoric acid;Obtain 2-amino ethyl cyanoacetate (B) by reduction, in turn with acetic anhydride, lawson reagent is reacted, and 5-amino-4-carboxylate thiazole compound (D) is obtained;After bromination by NBS, under the catalysis of phosphorus oxychloride, react with tetrahydropyridine indole ketone, generate 2-bromo-6,7-dihydrothiazolo [5'',4'':4',5'] pyrimido [1',2':1,2] pyrindo [3,4-b] indole-4 (12H) -ketone (F), again under alkaline condition, first with Boc piperazine, then by Boc treatment and obtain compound (H);Finally, react with acyl chloride, and 28 different substitution structures of five-ring thiazole indole ketone piperazine amide compound (I1-I28) are synthesized.The 28 compounds are tested on three kinds of cancer cells, and the results show that: 28 compounds have significant inhibitory activity on Hela cervical cancer cells, HT-29 human colon cancer cells and HGC-27 human gastric cancer cells.

This invention belongs to the field of organic synthesis and relates to a nitrogen-containing [3,2-b]indole derivative, its preparation method, and its application. The nitrogen-containing [3,2-b]indole derivative is a compound, optical isomer, or pharmaceutically acceptable salt thereof, as shown in the following structure: ; wherein R1, R2, R3, and R4 are independently selected from hydrogen atoms, halogens, cyano groups, C1-C8 alkyl groups, and C1-C8 alkoxy groups; R5, R6, R7, and R8 are independently selected from hydrogen atoms, halogens, and C1-C8 alkyl groups; R9 is selected from C1-C8 alkyl groups; when R1, R2, R3, R4, R5, R6, R7, and R8 are all hydrogen atoms, R9 is selected from C1-C2 alkyl groups. The compound exhibits antitumor activity, particularly against HeLa cervical cancer, showing a significant inhibitory effect on its proliferation.

The application discloses a dihydroquinazolinone-containing N-hydroxy amide compound and a preparation method and application thereof, relates to the technical field of medicinal chemistry, and utilizes the splicing principle to design and synthesize dihydroquinazolinone-containing N-hydroxy amide compounds with novel structures, and the compounds are tested in terms of HDAC6 protein affinity and anti-proliferation activity of cervical cancer cell Siha, and the results show that the compounds E1-E5 have better affinity to the HDAC6 protein, the IC 50 values are 4.1-85.4 nM, wherein the affinity of the compound E4 to the HDAC6 protein is higher than that of a positive control SAHA; and the compounds E1-E5 can better inhibit the proliferation of the cervical cancer cell Siha, the IC 50 values are 2.4-9.3 muM, wherein the activity of the compound E4 is the strongest.

This invention belongs to the field of pharmaceutical preparation technology, specifically relating to an HDAC inhibitor based on a tyrosine backbone, its synthesis method, and its application. The invention uses L-tyrosine as the backbone, with its aromatic ring as the Cap region, linked by a flexible alkyl chain via an ether bond, and terminated by an isohydroxamic acid group (ZBG). The tyrosine backbone provides a rigid structure, reducing entropy loss and enhancing hydrophobic interactions with the HDAC surface. By introducing substituents (such as halogens, methyl groups, etc.) onto the tyrosine benzene ring, the affinity with the HDAC active pocket is further optimized. The prepared compound b19 exhibits an HDAC inhibition capacity of 100%. 50 The concentration reached 26.8 nM, exhibiting superior antitumor activity compared to SAHA, especially against solid tumors such as HeLa cells with IC50. 50 It is 0.55 μM.

The present application relates to the technical field of biotechnology, and provides a CRISPR-Cas9 system sgRNA sequence capable of efficiently targeting and knocking out a human SPARC gene, a recombinant vector containing the sgRNA sequence, and a construction method and application demonstration of a SPARC gene knockout cell model.The sgRNA sequence can mediate a high-specificity and high-efficiency SPARC gene knockout effect, effectively solving the problems of strong blindness, low screening efficiency and lack of reliable tool sequences in the prior art sgRNA design for the gene.The SPARC gene knockout Hela cell line constructed by using the sgRNA sequence and the recombinant vector has important technical value and practical significance for studying the extracellular matrix network regulation and cell migration ability.

This invention relates to the field of camel thorn separation and purification technology, specifically to unsaturated ketone components from camel thorn, their separation and extraction methods, and their applications. The unsaturated ketone component in camel thorn is (7S,3E,5Z)-7,8-dihydroxy-6-methylocta-3,5-dien-2-one. This invention discloses for the first time the unsaturated ketone components in camel thorn, and in vitro antitumor pharmacodynamic experiments were conducted on these components. The experiments showed that the unsaturated ketone components in camel thorn have a certain inhibitory effect on human HeLa cervical cancer cells, thus enabling their application in the preparation of drugs for the prevention / treatment of cervical cancer.

The present application relates to a kind of preparation method and application of HPV recombinant protein therapeutic vaccine, belong to biological medicine technical field, the preparation method of the HPV recombinant protein therapeutic vaccine described includes: a plurality of complex lipid nanoparticles prepared by lipid are simultaneously wrapped and delivered HPV16HPV E6E7 recombinant protein antigen and nucleic acid TLR agonist adjuvant molecule.This application is beneficial to the recombinant protein therapeutic vaccine prepared to induce strong T cell immune response to HPV16E6E7 antigen, kill the cervical cancer cell infected by HPV.In addition, unlike the lipid nanoparticle (LNP) of mRNA vaccine delivery, there is no cation or ionizable lipid in the formula, significantly higher than mRNA vaccine in safety, with greater advantage than mRNA therapeutic vaccine, and low toxicity and side effects, vaccine effect is stable.

This invention belongs to the field of cell detection technology, specifically involving the application of NR4A1 as an early biomarker for gamma-ray radiation. This invention discovers that in the early stage (2 hours) after gamma-ray radiation, the protein and mRNA levels of NR4A1 are significantly increased, exhibiting a radiation dose-dependent effect. NR4A1 can serve as a 2-hour biomarker for gamma-ray irradiated HeLa cells, allowing for the screening of cells irradiated for 2 hours, differentiating the duration of gamma-ray irradiation, and achieving early diagnosis of cellular gamma-ray radiation.

The application discloses a butyl tin-cycloiridium phenanthroline complex with an aggregation-induced emission (AIE) property, a preparation method of the complex and anticancer application of the complex. The structural formula of the complex is shown as formula (I), and R is one of hydrogen, a phenyl group, a p-tolyl group, a p-bromophenyl group, a p-fluorophenyl group, a biphenyl group, a triphenylamine group, a carbazole group, a tetraphenyl ethene group and a triphenylbenzene group. The growth inhibition rates of the target compound on human alveolar basal epithelial carcinoma cells (A549) and cisplatin-resistant cells (A549 / DDP), cervical cancer cells (Hela) and human lung normal epithelial cells (BEAS-2B) are tested, and it is found through comparison that the target compound shows potential anticancer activity. In addition, the target compound shows unique AIE emission characteristics. The target compound mainly targets the lysosomes of A549 cells, and influences the mitochondria to cause the decline of the mitochondrial membrane potential, thereby showing anticancer activity.

The application relates to the technical field of pharmaceutical chemistry, and discloses a sulfuryl hydrazone compound, a preparation method thereof and anti-tumor application. The compound is synthesized from isatin derivatives and sulfuryl hydrazine through two-step nucleophilic substitution-elimination reaction, has a chemical structure as shown in formula (I), and is characterized by 1H NMR, 13C NMR and HRMS. The sulfuryl hydrazone compound has significant inhibitory activity on A549 (lung cancer), HepG2 (liver cancer) and Hela (cervical cancer) three human cancer cell strains, the value of some compounds is as low as 0.03 micromole / L, the compound can induce cell apoptosis by mediating ROS generation in tumor cells, inhibiting the NF-kappa B signal pathway and activating Caspase-3 activity, and can effectively inhibit cancer cell migration and colony formation. The compound has a mild synthesis process, simple operation and wide substrate applicability, can be used as a potential anti-tumor active ingredient, can be used for preparing drugs for resisting lung cancer, liver cancer, cervical cancer and other tumors, and can provide new candidate compounds and technical support for cancer treatment.

The application discloses an alpha-linolenic acid and metal iridium coupling compound, a preparation method and application thereof, and relates to the field of medicine, in particular to an alpha-linolenic acid and metal iridium coupling compound, which has good antitumor activity on human cervical cancer cells, human hepatoma cells and mouse breast cancer cells. The preparation method is simple and easy to operate, and the product has good antitumor activity.

The application belongs to the field of fluorescent probes, and relates to a lysosome-targeting fluorescent probe and a preparation method and application thereof. An intermediate is prepared by mixing an aldehyde compound, 5-amino fluorescein and 7-bromoheptanoic acid, and then performing a reaction; the intermediate is mixed with morpholine, and then a reaction is performed, so that the lysosome-targeting fluorescent probe is obtained. The application belongs to the construction of a complex molecular system with flexible and modular Ugi four-component reaction, adopts a one-pot method, and is synthesized by a framework template conjugation method, and the yield of the product is between 60% and 80%. The fluorescent probe prepared in the application can target lysosomes, and can be used as a lysosome-targeting imaging agent for living cancer cells. The fluorescent probe containing ferrocene prepared in the application can target lysosomes, and can be used as a lysosome-targeting imaging agent and a therapeutic agent for living cancer cells. The fluorescent probe containing ferrocene prepared in the application can effectively inhibit the growth of Hela and other cancer cells.

This invention discloses an indoline fused heterocyclic compound, its preparation method, and its applications, belonging to the field of pharmaceutical synthesis technology. The method includes the following steps: using quinone imine monoketal and α,β-unsaturated cycloalkenyl ether as raw materials, and employing a Brønsted acid-phosphonic acid catalyst, a Brønsted acid-catalyzed [3+2] tandem cyclization reaction is carried out to obtain the indoline fused heterocyclic compound. The method provided by this invention features readily available raw materials and mild conditions, while also exhibiting high regioselectivity, chemoselectivity, and atom economy. The product can be efficiently separated by column chromatography, providing an excellent pathway for the large-scale preparation of this type of compound. Furthermore, the indoline fused heterocyclic compound provided by this invention has a significant inhibitory effect on the growth of human cervical cancer cells (HeLa), demonstrating good antitumor biological activity, which is of great positive significance for promoting the development of antitumor drugs.

This invention relates to the field of antitumor drug technology, and discloses a cervical cancer treatment composition based on β-elemene and its preparation method. The composition comprises β-elemene, dimethyl sulfoxide (DMSO), and a diluent matrix; wherein the final concentration of β-elemene is 10 μg / mL to 50 μg / mL, and the volume percentage of DMSO is constant at 0.4% to 0.6%. The preparation method includes preparing a stock solution and preparing the final product. By dynamically adjusting the volume of DMSO added, the total amount of solvent in the composition at different drug concentrations remains constant, thus establishing a strict single-variable evaluation system for in vitro drug delivery. This invention effectively eliminates the cytotoxicity additive interference caused by fluctuations in the amount of solubilizer in conventional drug delivery systems, prevents the aggregation and precipitation of poorly soluble components in the aqueous phase, and can objectively and accurately reflect the true pharmacological activity of β-elemene in inhibiting cervical cancer cells and inducing ferroptosis.

The application discloses application of TGM2 as a cervical cancer diagnosis, prognosis biomarker and therapeutic target. The application provides a new cervical cancer diagnosis and prognosis marker TGM2, which can clearly characterize the occurrence and development of cervical cancer; the marker presents specific high expression phenomenon in comparison with normal tissue in human clinical cervical cancer tissue; and the marker also presents high expression in human cervical cancer cell lines. Knocking down TGM2 can inhibit the proliferation and migration ability of cervical cancer cells; TGM2, as an enzyme, has high application potential as a diagnosis and treatment target. The application provides a new marker and target for the diagnosis, prognosis and treatment of cervical cancer, and can provide a new strategy and direction for the treatment of cervical cancer.

本发明属于化学传感技术领域，具体涉及一种基于逻辑门检测 HeLa 细胞内阴离子的 NBD 锌配合物双模式光学探针及其制备方法和应用。所述传探针分子式为Zn(NBD‑BPHA)I2，其合成方法简单，实施可行性高，成本低廉，灵敏度、选择性、稳定性高，可同时检测丙二酸根、草酸根和焦磷酸根阴离子，为丙二酸根、草酸根和焦磷酸根阴离子检测领域的应用提供了一种新方法。

The present application belongs to the field of biotechnology, and relates to a method for producing coxsackie virus by using HeLa cells. Specifically, the present application relates to a method for culturing HeLa cells in vitro to produce coxsackie virus, which comprises: sequentially culturing HeLa cells in culture medium III and culture medium I, inoculating virus and culturing to obtain a cell culture containing coxsackie virus; wherein the culture medium III is a mixture of culture medium II and VirusPro culture medium at a volume ratio of 3:1-1:1, the culture medium I is a mixture of culture medium II and VirusPro culture medium at a volume ratio of 3:1-1:3, and the culture medium III and the culture medium I contain L-alanyl-L-glutamine.

A Shigella vaccine preparation comprising 10E8-10E12 CFU of a live, genetically attenuated Shigella flexneri strain that comprises a chromosomal deletion of setBA and which is non-invasive as determined by the Sereny test and an in vitro invasion assay using HeLa cells, wherein the strain comprises an endogenous invasion plasmid that is genetically engineered to incorporate a heterologous expression construct expressing a pathogen-specific antigen.

The application discloses matrine 14-position spiro derivative and a preparation method and application thereof, and belongs to the technical field of pharmacy. The application takes matrine as raw material, synthesizes matrine diazonium at the 14-position as a reaction intermediate, and synthesizes 26 matrine 14-position spiro derivatives through [3+2] cycloaddition reaction with acrylic ester compounds at normal temperature without a catalyst. The synthesized compounds are subjected to preliminary screening of in-vitro anti-tumor activity by selecting human liver cancer cells HepG2, human cervical cancer cells Hela and rat neuroglioma cells C6. The target compounds all show good anti-tumor activity, indicating that the compounds are anti-tumor drugs with development prospects, and can provide a reference for further development of matrine as an anti-tumor drug.

The present invention discloses a phenazine-1-carboxylic acid modified cyclometalated iridium(III) complex, its preparation method and application. This complex is formed by connecting cyclometalated iridium(III) with the natural small molecule phenazine-1-carboxylic acid, and has good anti-tumor activity against HeLa, HepG2, A549, and A549R cell lines. The preparation method of the metal complex provided by the present invention is simple and has good application prospects in anti-tumor research.

This invention relates to a siRNA that targets and inhibits the expression of the FAM195A gene and its applications, belonging to the field of biomedical technology. This invention designs and synthesizes siRNA that inhibits the expression of the FAM195A gene. The siRNA is siFAM195A, comprising a sense strand and an antisense strand; wherein the sense strand is shown in SEQ ID NO.1, and the antisense strand is shown in SEQ ID NO.2. This invention, by transfecting siFAM195A into cervical cancer cells, can effectively inhibit the expression of the FAM195A gene, effectively inhibit the growth and proliferation of cervical cancer cells, and promote apoptosis of cervical cancer cells. This invention provides a new potential drug for the treatment of cervical cancer, possessing high clinical application prospects and value.

This invention discloses a triazine-based fluorescent probe compound YJA, its preparation method, and its applications. The chemical name is 4'-(4,6-diphenyl-1,3,5-triazine-2-yl)-[1,1'-biphenyl]-4-yldiphenylphosphine ester, and the molecular formula is C2. 39 H 28 N3O2P. This invention prepares a novel triazine ring-based fluorescent probe, YJA, which exhibits advantages such as ultra-low detection limit (0.73 nM), rapid response, excellent selectivity, good pH stability (pH 4-11), and low cytotoxicity. It has been successfully applied to exogenous ONOO in HeLa cells. ‑ Fluorescence imaging and ONOO based on test strips ‑ Simple, visual, and rapid detection. The preparation method of this fluorescent probe is simple, uses readily available raw materials, and yields a white solid product that is easy to store. It has broad application prospects and can be mass-produced and applied.

This invention relates to the field of biomedical technology, and discloses an aromatic polyketide compound, its preparation method, and its applications. The aromatic polyketide compound is derived from... Crystallomyces hypericus Compound I and Compound II were isolated from the ethyl acetate extract of rice fermentation broth and identified as Crystalloketide A and Crystallomycin D, respectively. In vitro anticancer activity tests were conducted on Crystalloketide A and Crystallomycin D. The results showed that Compound I and Compound II inhibited human cervical cancer cells, human liver cancer cells, human neurocancer cells, and human pancreatic cancer cells, respectively. Therefore, it is further suggested that Compound I and Compound II described in this invention can be used to prepare anticancer drugs.

The application discloses a HeLa cell three-dimensional culture model, a preparation method and application. HeLa cells are mixed with a collagen-sodium alginate composite hydrogel precursor solution to obtain a cell-precursor mixture, the cell-precursor mixture is mixed with a CaCl2 solution to obtain a mixed solution, and the mixed solution is placed in an incubator for standing culture for 10-60 min, so that the hydrogel is cross-linked and solidified to form a three-dimensional culture system; complete culture medium is further added for continuous culture, and a HeLa cell three-dimensional culture model is constructed. The HeLa cell three-dimensional culture model is constructed by using the hydrogel, defects existing in simulation of a tumor microenvironment by two-dimensional culture and an animal model are effectively solved, the body microenvironment can be better simulated, cells form regular three-dimensional cell spheroids, a malignant phenotype is maintained, the accuracy and repeatability of drug screening are improved, and a reliable technical platform is provided for cervical cancer mechanism research and anti-tumor drug screening.

This invention relates to the field of Lithospermum erythrorhizon isolation and purification technology, specifically a method for extracting and separating benzoquinone components from Lithospermum erythrorhizon in Xinjiang, and its application in antitumor drugs. The method involves extracting and concentrating the dried roots of Lithospermum erythrorhizon by ethanol reflux. The concentrated extract is then further extracted and concentrated. The resulting petroleum ether fraction is subjected to chromatographic separation and gradient elution, followed by colorimetric analysis. Ten similar components are combined. The first concentrate is then separated, subjected to gradient elution, and subjected to colorimetric analysis, resulting in the combination of seven similar components. The second concentrate is then separated, subjected to gradient elution, and subjected to colorimetric analysis, resulting in the combination of nine similar components. The third concentrate is then separated and purified to obtain the final product. This invention features a simple process, environmental friendliness, and high product purity. It is the first time that benzoquinone components from Lithospermum erythrorhizon in Xinjiang have been obtained. In vitro activity verification has shown that these components exhibit inhibitory activity against HeLa tumor cells, and they can be applied to the extraction and separation of drugs for the prevention and / or anti-cervical cancer.

This invention relates to the field of antitumor compound technology, providing a cyclic alphatin saponin compound, its extraction and purification method, and its applications. This invention is the first to extract, isolate, and identify a cyclic alphatin saponin compound (aspleniumside J) from the traditional Chinese medicine *Fernonia acutissima*. In vitro antitumor studies revealed that aspleniumside J can inhibit the proliferation of MDA-MB-231, HCC-1937, HeLa, and HepG2 cell lines, especially showing high activity against HepG2 liver cancer cells. Simultaneously, aspleniumside J can inhibit cell cycle arrest in the S phase of HepG2 liver cancer cells. In summary, the novel cyclic alphatin saponin compound provided by this invention exhibits high antitumor activity and has broad application prospects in the field of antitumor drugs, especially in the field of anti-liver cancer drugs.

This invention discloses the application of a 4-methylenemalonium-benzopyran derivative fluorescent dye in biofluorescence imaging. The 4-methylenemalonium-benzopyran derivative fluorescent dye, as shown in formula (I), is obtained by a Knoevenagel condensation reaction of 9-aldehyde julonidine with 2-(2-methyl-4H-benzopyran-4-methylene)malonium under piperidine catalysis. The preparation method of this compound is simple, the reaction conditions are mild, and the yield is high. The 4-methylenemalonium-benzopyran derivative fluorescent dye exhibits excellent two-photon absorption properties and can be applied to confocal fluorescence imaging of HeLa cells and two-photon excitation microscopy of HEK cells. Furthermore, this compound has good biocompatibility and can achieve mitochondrial localization, showing great promise for applications in molecular biology and medicine.

This invention discloses the application of albumin-loaded drug-eluting nanoparticles for dual-phototherapy in the preparation of drugs for treating cervical cancer, belonging to the field of pharmaceutical technology. The albumin-loaded drug-eluting nanoparticles of this invention are formed by loading indocyanine green and hydroxyalizarin onto albumin; the nanoparticle system simultaneously contains ICG and purpurin; the ICG photothermal agent can not only convert light energy into heat energy through photothermal action, but also generate reactive oxygen species through photodynamic therapy to inhibit cell activity, while purpurin inhibits GDH1 activity, disrupting intracellular redox homeostasis and cutting off the nutrient supply to cancer cells. Based on the above three anti-tumor mechanisms, this invention overcomes the shortcomings of single-treatment chemotherapy, such as high toxicity and low therapeutic effect. By combining the nanosystem with photodynamic and photothermal therapy, the therapeutic effect of hydroxyalizarin on cervical cancer cells is improved, providing a new solution to the technical challenges of high recurrence and mortality rates in cervical cancer.