115 results about "HeLa" patented technology

The invention relates to the technical field of biological medicine, and discloses an aromatic polyketone compound as well as a preparation method and application thereof. According to the aromatic polyketone compound, a compound I and a compound II are separated from an ethyl acetate extract of rice fermentation liquor of Crystalomyces hyricus, and the compound I and the compound II are respectively named as Crystaloketide A and Crystalomycin D. In-vitro anti-cancer activity tests are carried out on the Crystaloketide A and the Crystalomycin D. According to the aromatic polyketone compound disclosed by the invention, the anti-cancer activity of the Crystaloketide A and the Crystalomycin D. Results show that the compound I and the compound II have the effects of inhibiting human cervical cancer cells, human liver cancer cells, human nerve cancer cells and human pancreatic cancer cells respectively, so that the compound I and the compound II can be further prompted to be used for preparing anti-cancer drugs.

The invention discloses application of a tripterygium wilfordii exosome in preparation of an anti-cervical cancer medicine and a tumor angiogenesis inhibiting medicine, and relates to the field of biological medicine. The medicinal plant exosome derived from tripterygium wilfordii is successfully separated, and it is proved that the medicinal plant exosome can regulate related pathways through delivery of functional nucleic acid molecules to inhibit proliferation and migration of cervical cancer cells and promote active oxygen related apoptosis so as to be used for tumor treatment. The tripterygium wilfordii exosome can further act on vascular endothelial cells in a tumor microenvironment, the migration function of Hela cells is inhibited, and then the effect of resisting cell proliferation in the tumor microenvironment is achieved. The tripterygium wilfordii exosome has the advantages of being simple in preparation method, remarkable in tumor growth resistance and tumor cell migration inhibition effect, good in biocompatibility, high in delivery efficiency and the like, and can be used as a novel nano-drug for tumor treatment.

The invention relates to the technical field of medical information processing, in particular to a cervical cancer cell early screening and intelligent evaluation system based on multi-modal data, which comprises a data acquisition module, a form detection module and a screening early warning module, the data acquisition module is used for acquiring multi-modal data of a current detection object in a plurality of inspection processes; the morphology detection module is used for calculating a cellular morphology score of a current detection object in any target inspection process; the screening early warning module is used for determining similar detection objects of the current detection object, and correcting differences between the current detection object and the similar detection objects in the aspects of cellular morphology scores and lesion detection indexes according to the approximation degree between different types of lesion detection indexes of the current detection object; and obtaining the screening early warning coefficient of the current detection object by using the relative change condition of the correction result. According to the invention, the early screening and evaluation effects on cervical cancer cells are improved.

The present application relates to a kind of five-ring thiazole indole ketone piperazine amide derivatives and purposes thereof.The derivatives take ethyl cyanoacetate as starting material, generate hydroxylamine compound (A) under the action of sodium nitrite and phosphoric acid;Obtain 2-amino ethyl cyanoacetate (B) by reduction, in turn with acetic anhydride, lawson reagent is reacted, and 5-amino-4-carboxylate thiazole compound (D) is obtained;After bromination by NBS, under the catalysis of phosphorus oxychloride, react with tetrahydropyridine indole ketone, generate 2-bromo-6,7-dihydrothiazolo [5'',4'':4',5'] pyrimido [1',2':1,2] pyrindo [3,4-b] indole-4 (12H) -ketone (F), again under alkaline condition, first with Boc piperazine, then by Boc treatment and obtain compound (H);Finally, react with acyl chloride, and 28 different substitution structures of five-ring thiazole indole ketone piperazine amide compound (I1-I28) are synthesized.The 28 compounds are tested on three kinds of cancer cells, and the results show that: 28 compounds have significant inhibitory activity on Hela cervical cancer cells, HT-29 human colon cancer cells and HGC-27 human gastric cancer cells.

This invention belongs to the field of organic synthesis and relates to a nitrogen-containing [3,2-b]indole derivative, its preparation method, and its application. The nitrogen-containing [3,2-b]indole derivative is a compound, optical isomer, or pharmaceutically acceptable salt thereof, as shown in the following structure: ; wherein R1, R2, R3, and R4 are independently selected from hydrogen atoms, halogens, cyano groups, C1-C8 alkyl groups, and C1-C8 alkoxy groups; R5, R6, R7, and R8 are independently selected from hydrogen atoms, halogens, and C1-C8 alkyl groups; R9 is selected from C1-C8 alkyl groups; when R1, R2, R3, R4, R5, R6, R7, and R8 are all hydrogen atoms, R9 is selected from C1-C2 alkyl groups. The compound exhibits antitumor activity, particularly against HeLa cervical cancer, showing a significant inhibitory effect on its proliferation.

The application discloses a dihydroquinazolinone-containing N-hydroxy amide compound and a preparation method and application thereof, relates to the technical field of medicinal chemistry, and utilizes the splicing principle to design and synthesize dihydroquinazolinone-containing N-hydroxy amide compounds with novel structures, and the compounds are tested in terms of HDAC6 protein affinity and anti-proliferation activity of cervical cancer cell Siha, and the results show that the compounds E1-E5 have better affinity to the HDAC6 protein, the IC 50 values are 4.1-85.4 nM, wherein the affinity of the compound E4 to the HDAC6 protein is higher than that of a positive control SAHA; and the compounds E1-E5 can better inhibit the proliferation of the cervical cancer cell Siha, the IC 50 values are 2.4-9.3 muM, wherein the activity of the compound E4 is the strongest.

The invention discloses a supramolecular self-assembled fiber as well as a preparation method and application thereof in cells. The supramolecular self-assembled fiber is obtained by mixing a cis, cis-cyclohexane-1, 3, 5-triformyl hydrazine mother solution, a p-formylphenylboronic acid mother solution and a 5-(2-aminophenyl) tetrazole mother solution in a phosphate buffer solution; the application of the supramolecular self-assembled fiber in cells comprises the following steps: inoculating a pore plate with Haila cells, gently and horizontally shaking, culturing, washing, incubating in a complete DMEM (Dulbecco Modified Eagle Medium) solution of 5-(2-aminophenyl) tetrazole, and washing to obtain pretreated cells; and further incubating and washing the pretreated cells in a complete DMEM (dulbecco's modified eagle medium) solution containing cis, cis-cyclohexane-1, 3, 5-triformyl hydrazine and p-formylphenylboronic acid, so as to obtain the cells enriched with the boron element. Compared with a small molecular boron carrying agent, the supramolecular self-assembled fiber prepared by the invention realizes effective boron element enrichment, and has the advantages of high affinity to tumor cells, good stability and low biotoxicity.

This invention belongs to the field of biomedical technology, and particularly relates to a pharmaceutical composition for treating cervical cancer and its preparation method. The pharmaceutical composition for treating cervical cancer of this invention comprises the following components: a apigenin derivative and acetylvalerin in a mass ratio of 1:(0.3~0.5). This invention obtains a apigenin derivative by introducing specific functional groups onto apigenin, which exhibits high pharmacological activity against cervical cancer; synergistically acting with acetylvalerin, it effectively kills cervical cancer cells while reducing damage to normal cells, thus providing a safer and more effective treatment option for cervical cancer patients.

The invention discloses an mRNA (messenger Ribonucleic Acid) quality control product of human papilloma virus as well as a preparation method and application thereof, and belongs to the technical field of in-vitro diagnosis quality control. The method comprises the following steps: chemically synthesizing an HPV E6 / E7 gene DNA sequence, respectively cloning the HPV E6 / E7 gene DNA sequence into a cloning vector to construct a synthetic plasmid template, further preparing a nucleic acid fragment containing E6 / E7 mRNA through in-vitro transcription, carrying out absolute quantification through a digital PCR technology, mixing the nucleic acid fragment with human cervical cancer cell line RNA without any HPV sequence, and screening to obtain the HPV E6 / E7 gene DNA sequence. And the mRNA quality control product with stable structure and accurate value is prepared through a vacuum freeze drying technology. The quality control product can simulate HPV E6 / E7 mRNA in a clinical sample, has comprehensive types, is suitable for accurate evaluation of sensitivity, specificity, precision and other analysis performances of an HPV E6 / E7 mRNA detection kit, provides a key quality control tool for accurate risk stratification of cervical cancer, and is suitable for various detection platforms.

The application relates to a kind of mitochondria-based single cell feature extraction and analysis method and system, comprising: obtaining the multiple modal images such as bright field image, nucleus fluorescent image and mitochondria fluorescent image of single cell;Image preprocessing is carried out to the three modal images obtained;For different structures such as mitochondria, morphological and texture features are extracted, and feature analysis is carried out;Further, through the fusion of mitochondria and machine learning technology, the automatic classification of cells is realized.The application is used for the classification of human cervical epithelial cells (H8) and cervical cancer cells (HeLa), and the machine learning analysis of morphological features and texture features shows the potential of mitochondria in the classification of cervical cells.The application has strong applicability, can be combined with machine learning and other analysis methods, and can be applied to various biological cells, has universality, and is easy to popularize.

This invention belongs to the field of pharmaceutical preparation technology, specifically relating to an HDAC inhibitor based on a tyrosine backbone, its synthesis method, and its application. The invention uses L-tyrosine as the backbone, with its aromatic ring as the Cap region, linked by a flexible alkyl chain via an ether bond, and terminated by an isohydroxamic acid group (ZBG). The tyrosine backbone provides a rigid structure, reducing entropy loss and enhancing hydrophobic interactions with the HDAC surface. By introducing substituents (such as halogens, methyl groups, etc.) onto the tyrosine benzene ring, the affinity with the HDAC active pocket is further optimized. The prepared compound b19 exhibits an HDAC inhibition capacity of 100%. 50 The concentration reached 26.8 nM, exhibiting superior antitumor activity compared to SAHA, especially against solid tumors such as HeLa cells with IC50. 50 It is 0.55 μM.

The invention discloses fluoroalkyl substituted azafluorene and pyridopyrimidino isoindole compounds as well as a synthesis method and application thereof, and belongs to the technical field of organic synthesis and drug discovery. The preparation method comprises the following steps: mixing a 3-alkenyl-1, 2, 4-oxadiazolone compound 1, a fluoroalkyl acetylenic ketone compound 2, a catalyst, an additive and an organic solvent, and carrying out heating reaction to prepare a fluoroalkyl substituted azafluorene compound 3 and / or a fluoroalkyl substituted pyridopyrimidino isoindole compound 4. Experimental research discovers that the compound disclosed by the invention has the activity of obviously inhibiting proliferation of Hela and HCT-116 cancer cells, so that the compound disclosed by the invention has obvious anti-cancer activity on cervical cancer and / or colon cancer, has potential medicinal value, and provides a new structural unit for drug screening.

The invention relates to the technical field of medicines, in particular to an enantiomer-atesane type diterpenoid compound as well as a preparation method and an application of the enantiomer-atesane type diterpenoid compound. The enantiomer-atesane diterpenoid compound disclosed by the invention is derived from plants, has anti-tumor activity, has cytotoxicity to human leukemia cells, human liver cancer cells and human cervical cancer cells, and is relatively good in inhibitory activity. Wherein the compound 3 has the best effect, the IC50 values of the compound 3 to three human tumor cells are 7.5 + / -1.2 micromole per liter, 9.8 + / -1.0 micromole per liter and 10.1 + / -0.9 micromole per liter respectively, and the activity of the compound 3 is equivalent to that of a positive control drug mitomycin. Particularly, the compound 3 also has good cytotoxicity to drug-resistant liver cancer cells (Huh7-LR cells), and the IC50 value of the compound 3 is 9.6 + / -1.1 micromole per liter. The compound 3 shows huge potential as a lead compound by virtue of the novel chemical structure and potent cytotoxicity shown in various cell lines.

The present application relates to the technical field of biotechnology, and provides a CRISPR-Cas9 system sgRNA sequence capable of efficiently targeting and knocking out a human SPARC gene, a recombinant vector containing the sgRNA sequence, and a construction method and application demonstration of a SPARC gene knockout cell model.The sgRNA sequence can mediate a high-specificity and high-efficiency SPARC gene knockout effect, effectively solving the problems of strong blindness, low screening efficiency and lack of reliable tool sequences in the prior art sgRNA design for the gene.The SPARC gene knockout Hela cell line constructed by using the sgRNA sequence and the recombinant vector has important technical value and practical significance for studying the extracellular matrix network regulation and cell migration ability.

The invention provides a dual-targeting nano-material photosensitizer for cervical cancer as well as a preparation method and application of the dual-targeting nano-material photosensitizer. The folic acid modified poly-beta-cyclodextrin (poly-beta-CD) is used as a nano-carrier and is used for loading a photosensitizer PAP (PAP is pyropheophorbide a covalently connected PAAKRVKLD) coupled with a nuclear localization signal (NLSs). The finally prepared FA-CD-coated PAP nano material can specifically recognize cervical cancer cells of overexpressed folate receptors (FR), so that accumulation in tumor cells is remarkably enhanced. In addition, the encapsulated PAP can achieve accurate cell nucleus localization. Under an illumination condition, reactive oxygen species (ROS) generated by PAP accumulated in nuclei can instantly oxidize and damage a DNA chain or DNA repair enzyme, so that cell death is directly induced, and the anti-tumor effect of targeted photodynamic is greatly enhanced. The novel dual-targeting nano drug-loading system prepared by the invention solves the problems of poor targeting and biological solubility and limited treatment effect of a traditional photosensitizer, and provides an innovative intelligent targeting delivery strategy for in-situ cell nucleus photodynamic therapy.

The application discloses a beta-galactosidase near-infrared fluorescent probe with a large stokes shift and a preparation method and application thereof. The structure of CPD-gal is shown in formula I. When a 580nm excitation wavelength is used for excitation, CPD-gal has weak fluorescence at 713nm, but can selectively undergo a fluorescence opening reaction with beta-gal. With the increase of the concentration of beta-gal, the fluorescence intensity at 713nm is gradually enhanced, and other interfering ions and species will not interfere with the detection of beta-gal by CPD-gal. Since the emission wavelength is in the near-infrared region and a large stokes shift is generated, the system background fluorescence interference is greatly reduced, and the sensitivity of the detection of beta-gal is improved. Therefore, CPD-gal is suitable for the fluorescence detection of beta-gal with high selectivity and high sensitivity. The probe is successfully applied to the fluorescence imaging of endogenous beta-gal in HeLa cells and SK-OV-3 cells.

This invention relates to the field of camel thorn separation and purification technology, specifically to unsaturated ketone components from camel thorn, their separation and extraction methods, and their applications. The unsaturated ketone component in camel thorn is (7S,3E,5Z)-7,8-dihydroxy-6-methylocta-3,5-dien-2-one. This invention discloses for the first time the unsaturated ketone components in camel thorn, and in vitro antitumor pharmacodynamic experiments were conducted on these components. The experiments showed that the unsaturated ketone components in camel thorn have a certain inhibitory effect on human HeLa cervical cancer cells, thus enabling their application in the preparation of drugs for the prevention / treatment of cervical cancer.

The application discloses a preparation method of a graphene quantum dot solution for HeLa cell imaging, and comprises the following steps: taking waste silicon material and waste fluorinated plastic as a basic precursor, taking sodium chloride as a diluent, and preparing graphite nanometer carbon balls by applying a certain intensity of current and voltage to initiate a self-driven chemical reaction; and after the graphite nanometer carbon balls are purified, oxidation peeling and dialysis are performed to obtain the graphene quantum dot solution. The preparation method is simple, low in cost, clear in imaging effect, stable in luminescence property, and capable of controlling the diameter of quantum dots by adjusting the adding amount of the diluent, so that the fluorescence of different colors can be controlled, and can be used for multicolor labeling imaging of cells. The graphene quantum dots prepared by the application can be well attached to the surface of HeLa cells and present significant fluorescence characteristics. Compared with other quantum dot synthesis processes, the graphene quantum dots prepared by the application are wide in raw material sources, economical, efficient, adjustable in quantum dot particle size, and high in yield.

The application relates to the technical field of medical image database, and discloses a standardized cervical cancer pathological image database construction method, which comprises the following steps: step 1, specimen number is hashed to obtain pseudo-anonymous specimen number, unique slice identification and cell-level unique identification are generated; step 2, a unified reference system is established, the reference layer pixel spacing, the down-sampling multiple and the coordinate mapping are determined; step 3, the image is converted into an optical density space and normalized according to a target staining base to obtain a standardized image; step 4, cross-modal anchor point lists are established by manually comparing cytological and histological images; step 5, a hierarchical label set is generated by synthesizing multi-level unique labels according to the severity full sequence; step 6, the intersection-over-union index and the kappa consistency value are calculated according to the combination key, and a review to-do list is generated; step 7, point review and rule revision are carried out, and a data dictionary, a consistency report and an anchor point snapshot are output. The application realizes the standardized database construction and traceable quality control of cervical cancer cytological and histological pathological images.

The present application relates to a kind of preparation method and application of HPV recombinant protein therapeutic vaccine, belong to biological medicine technical field, the preparation method of the HPV recombinant protein therapeutic vaccine described includes: a plurality of complex lipid nanoparticles prepared by lipid are simultaneously wrapped and delivered HPV16HPV E6E7 recombinant protein antigen and nucleic acid TLR agonist adjuvant molecule.This application is beneficial to the recombinant protein therapeutic vaccine prepared to induce strong T cell immune response to HPV16E6E7 antigen, kill the cervical cancer cell infected by HPV.In addition, unlike the lipid nanoparticle (LNP) of mRNA vaccine delivery, there is no cation or ionizable lipid in the formula, significantly higher than mRNA vaccine in safety, with greater advantage than mRNA therapeutic vaccine, and low toxicity and side effects, vaccine effect is stable.

This invention belongs to the field of cell detection technology, specifically involving the application of NR4A1 as an early biomarker for gamma-ray radiation. This invention discovers that in the early stage (2 hours) after gamma-ray radiation, the protein and mRNA levels of NR4A1 are significantly increased, exhibiting a radiation dose-dependent effect. NR4A1 can serve as a 2-hour biomarker for gamma-ray irradiated HeLa cells, allowing for the screening of cells irradiated for 2 hours, differentiating the duration of gamma-ray irradiation, and achieving early diagnosis of cellular gamma-ray radiation.

The application discloses a butyl tin-cycloiridium phenanthroline complex with an aggregation-induced emission (AIE) property, a preparation method of the complex and anticancer application of the complex. The structural formula of the complex is shown as formula (I), and R is one of hydrogen, a phenyl group, a p-tolyl group, a p-bromophenyl group, a p-fluorophenyl group, a biphenyl group, a triphenylamine group, a carbazole group, a tetraphenyl ethene group and a triphenylbenzene group. The growth inhibition rates of the target compound on human alveolar basal epithelial carcinoma cells (A549) and cisplatin-resistant cells (A549 / DDP), cervical cancer cells (Hela) and human lung normal epithelial cells (BEAS-2B) are tested, and it is found through comparison that the target compound shows potential anticancer activity. In addition, the target compound shows unique AIE emission characteristics. The target compound mainly targets the lysosomes of A549 cells, and influences the mitochondria to cause the decline of the mitochondrial membrane potential, thereby showing anticancer activity.

The invention relates to the technical field of organic synthesis, in particular to a cyclic peptide toxin drg-linker as well as a preparation method and application thereof. According to the present invention, the coupling site of PABC and aspartic acid is subjected to drg-linker synthesis by using a nickel catalysis manner, and the obtained drg-linker has high activity, and has strong inhibition effects on a Hela cell line and an SKOV3 cell line;

The application relates to the technical field of pharmaceutical chemistry, and discloses a sulfuryl hydrazone compound, a preparation method thereof and anti-tumor application. The compound is synthesized from isatin derivatives and sulfuryl hydrazine through two-step nucleophilic substitution-elimination reaction, has a chemical structure as shown in formula (I), and is characterized by 1H NMR, 13C NMR and HRMS. The sulfuryl hydrazone compound has significant inhibitory activity on A549 (lung cancer), HepG2 (liver cancer) and Hela (cervical cancer) three human cancer cell strains, the value of some compounds is as low as 0.03 micromole / L, the compound can induce cell apoptosis by mediating ROS generation in tumor cells, inhibiting the NF-kappa B signal pathway and activating Caspase-3 activity, and can effectively inhibit cancer cell migration and colony formation. The compound has a mild synthesis process, simple operation and wide substrate applicability, can be used as a potential anti-tumor active ingredient, can be used for preparing drugs for resisting lung cancer, liver cancer, cervical cancer and other tumors, and can provide new candidate compounds and technical support for cancer treatment.

The present application relates to the technical field of microorganisms, in particular to a lactobacillus crispatus Lcr5-6 and application thereof, the lactobacillus crispatus Lcr5-6 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.38486;The adhesion rate of the lactobacillus crispatus Lcr5-6 to Hela cells is as high as 61.11%, which is significantly higher than that of the control strain lactobacillus delbrueckii DM8909 (12.13%), has excellent vaginal epithelial cell adhesion and colonization ability, and can be long-term colonization in the vagina to play a probiotic role.

The invention discloses application of MYSM1 regulation and control of ITPR1 mediated cell autophagy in inhibition of cervical cancer, and belongs to the technical field of biomedicine.The MYSM1 gene and / or expression or activity of encoded protein of the MYSM1 gene are / is up-regulated, and an MYSM1-ITPR1-autophagy signal channel is activated, so that proliferation, migration, invasion or epithelial-mesenchymal transition of cervical cancer cells is inhibited, and cervical cancer is inhibited. And / or promoting apoptosis of cervical cancer cells; wherein the MYSM1 activates the expression of ITPR1 through the activity of a deubiquitination enzyme which is subjected to single ubiquitination modification on the 119th lysine of histone H2A of the MYSM1, and the ITPR1 serves as an endoplasmic reticulum calcium ion channel to mediate calcium ion release so as to trigger cell autophagy. Based on the mechanism, the invention provides a new application of MYSM1 / ITPR1 in preparation of anti-cervical cancer drugs, a drug composition targeting the pathway, a candidate drug screening method and a kit for diagnosis and prognosis. The invention aims to solve the problem that a technical scheme for effectively inhibiting the malignant progression of cervical cancer by targeting an MYSM1-ITPR1 pathway is lacked in the prior art.

The invention relates to an aloe-emodin piperazine derivative as well as a preparation method and application thereof, and belongs to the technical field of medicine synthesis. The aloe-emodin piperazine derivative is obtained by taking aloe-emodin as a raw material, substituting 15-site alcoholic hydroxyl of the aloe-emodin with piperazine and then connecting the 15-site alcoholic hydroxyl with a natural compound with anti-tumor activity and a derivative of the natural compound by utilizing different connecting arms. In-vitro anti-tumor experiments of the aloe-emodin piperazine derivative show that the aloe-emodin piperazine derivative has good anti-tumor activity on human cervical cancer cells (HeLa), human liver cancer cells (HepG2), human lung cancer cells (A549) and human osteosarcoma cells (U2OS), is expected to be developed into drugs for treating cervical cancer, osteosarcoma, lung cancer or liver cancer and other cancers, and has wide clinical application prospects.

The invention discloses a benzo [b] azinone compound as well as a synthesis method and anticancer activity thereof, and belongs to the technical field of organic synthesis and drug discovery. The preparation method comprises the following steps: mixing an o-alkenyl aniline compound, a cyclopropenone compound, a catalyst, an additive and an organic solvent, heating, and carrying out a one-pot cascade reaction to prepare the benzo [b] azinone compound. The compounds with various substituents are screened, and anti-cancer cell activity tests prove that the compounds can obviously inhibit proliferation of three kinds of cancer cells, namely Hela, SW-480 and HCT-116, so that the compounds have obvious anti-cancer activity on cervical cancer and / or colon cancer and have potential medicinal value; meanwhile, the synthesis method is simple and efficient in process, and has the advantages that raw materials are cheap and easy to obtain, operation is easy and convenient, atom economy is high, the substrate application range is wide, and functional group tolerance is good.

The invention discloses a peony polyphenol extract as well as a preparation method and application thereof, and relates to the technical field of biological medicines. Dried flowers of paeonia ostii are used as raw materials, peony polyphenol powder is obtained through an ethanol extraction-pore resin purification method, and the polyphenol content of the peony polyphenol powder is larger than 80% through determination of a folin-phenol method. Paeonia suffruticosa polyphenol is rich in more than 30 kinds of main active ingredients, and comprises flavonoids, phenolic acids and polyphenol derivatives, and flavonoids and phenolic acid compounds account for 81.25% of the total number of identified ingredients. The peony polyphenol plays a role in resisting cervical cancer through double ways of inducing HeLa cell apoptosis and activating HeLa cell autophagy, and the problem of drug resistance of a single-target drug can be effectively solved; the compound has a good application value in anti-cancer drugs. Meanwhile, the peony polyphenol shows excellent antioxidant activity in various free radical models, remarkably inhibits ROS (reactive oxygen species) generation at a cellular level, relieves oxidative stress, has a cell protection effect, and can be used as a natural antioxidant and a cell protection agent.

The invention discloses a method for stereoselectively synthesizing a chiral triarylmethane thioether compound with antitumor biological activity, and relates to the technical field of medicine synthesis. According to the invention, a seven-membered ring system 3-indolyl [1, 4] sulfur azacycloheptane compound is used as a raw material, an asymmetric Friedel-Crafts alkylation reaction with nucleophilic indole is realized through a new strategy that a spiro chiral phosphonic acid catalyst catalyzes bond breaking of a carbon-nitrogen bond, a high-quality path is provided for synthesis of a chiral triarylmethane thioether compound, and the chiral triarylmethane thioether compound has a wide application prospect. Meanwhile, the reaction has the characteristics of easily available raw materials and mild conditions, and also has the technical advantages of high enantioselectivity and high atom economy. The chiral triarylmethane thioether compound provided by the invention has an obvious inhibition effect on the growth of human cervical cancer cells (HeLa), pancreatic cancer cells (Panc-1) and human bone marrow neuroblastoma cells (Sy5Y), shows good anti-tumor biological activity, and has important positive significance for promoting the research and development of anti-tumor drugs.