171 results about "HPV infection" patented technology

The present application relates to a polynucleotide molecule that can be used for preventing or treatment HPV infection-related diseases, and a pharmaceutical product, a pharmaceutical composition, or an mRNA vaccine comprising said polynucleotide.

The invention relates to an application of a dihydropyridine compound or a salt thereof in preparation of a medicine for preventing and / or treating HPV infection, and belongs to the technical field of medicines and medicinal preparations. The invention provides application of a dihydropyridine compound or a salt thereof in preparation of a medicine for preventing and / or treating HPV (human papillomavirus) infection. The dihydropyridine compound is selected from one or more of nifedipine, amlodipine, lercanidipine, nimodipine, nitrendipine, nisoldipine, felodipine, benidipine, lacidipine and azelnidipine. According to the scheme, the application of the dihydropyridine compound or the salt thereof in prevention and / or treatment of HPV is reported for the first time, and an in-vitro cell experiment is carried out through azelnidipine in the dihydropyridine compound; furthermore, the inhibition effect on the HPV virus is verified through mouse intradermal vaccination virus model drug delivery and mouse vaginal vaccination virus model drug delivery.

The utility model discloses a medical instrument for treating cervicitis and cervical HPV (human papillomavirus) infection, which comprises a liquid medicine storage bag in which treatment medicine is stored, a conveying pipeline is fixedly arranged outside the front end of the liquid medicine storage bag, and a spraying head is arranged at the top end of the conveying pipeline. The spraying head is used for spraying out treatment liquid medicine to achieve the purpose of treating cervicitis and cervical HPV infection, handheld finger rings are installed on the left side and the right side of the root of the conveying pipeline, an installation ring is installed outside the conveying pipeline, and expansion mechanisms are installed on the left side and the right side of the installation ring. According to the medical instrument for treating cervicitis and cervical HPV infection, a novel structural design is adopted, an expansion mechanism is arranged, expansion plates on the left side and the right side of a conveying pipeline enter the vagina of a patient along with the device in the using process of the device, then the expansion plates on the left side and the right side rotate outwards, the vagina of the patient is expanded, and therefore the vagina of the patient is expanded. Therefore, friction between the conveying pipeline and the body of a patient when the conveying pipeline extends into the body is reduced, and discomfort of the patient is relieved.

The invention provides a primer probe group and a kit for early detection of high-grade cervical lesions and cervical cancer. The nucleotide sequence of the primer probe group is as shown in any one of SEQ ID NO: 1-69; or a nucleotide sequence obtained by modifying, substituting, deleting or adding one or more basic groups to the nucleotide sequence as shown in any one of SEQ ID NO: 1-69, or a nucleotide sequence with similar functions to the nucleotide sequence as shown in any one of SEQ ID NO: 1-69. The primer probe group supports household sampling of cervical specimens, is combined with high-risk HPV and polygene methylation for joint detection, has high sensitivity and specificity, can quickly screen cervical cancer and high-grade lesions, reduces the omission ratio and improves the detection accuracy. Especially, cervical cancer and high-grade lesions caused by non-HPV infection and non-high-risk HPV infection which cannot be detected by other screening methods can be detected. Unnecessary referral treatment is reduced, the operation process is simplified, the time and economic cost of the examinee are reduced, and meanwhile the medical burden is relieved.

The invention provides an anti-HPV (human papillomavirus) temperature-sensitive gel as well as a preparation method and application thereof. The invention provides the anti-HPV temperature-sensitive gel as well as the preparation method and application thereof. The gel consists of a natural active extract, a temperature-sensitive polymer, a cross-linking agent and a stabilizer, wherein the natural active extract comprises a mixture of a sophora flavescens extract, an artemisia annua extract and a mushroom extract in a specific mass ratio. These extracts are obtained by specific extraction processes to ensure their antiviral activity. The thermo-sensitive polymer is a mixture of poly (N-isopropylacrylamide) and hydroxypropyl methyl cellulose, and the cross-linking agent and the stabilizer are used for enhancing the performance of the gel. The preparation method of the gel comprises the following steps: preparing a natural active extract emulsion, preparing a wall material solution, emulsifying and microencapsulating, so as to finally form the gel with temperature sensitivity. The gel has specific rheological properties at body temperature, can be used for treating HPV infection, and provides a new local treatment method.

Embodiments relate to novel vaccines against human papillomavirus (HPV) and HPV-related diseases, including multiple types of cancers. The HPV vaccines are composed of anti-human dendritic cell (DC) surface receptor antibodies, including CD40, and E6 / 7 proteins of HPV16 and 18. The technology described is not limited to making vaccines against HPV16- and HPV18-related diseases and can be applied to making vaccines carrying E6 / 7 from any type of HPV. The HPV vaccines described can target DCs, major and professional antigen presenting cells (APCs), and can induce and activate potent HPV E6 / 7-specific and strong CD4+ and CD8+ T cell responses. The HPV vaccines can be used for the prevention of HPV infection and HPV-related diseases as well as for the treatment of HPV-related diseases, including cancers.

Compositions and methods are provided for treating HPV infections including pre-malignant and cancers. Compounds that specifically bind to the HPV E6 protein and inactivate the protein are disclosed.

A pharmaceutically acceptable salt of an acyclic nucleotide phosphoramidate to treat HPV and related conditions including neoplasia, as well as pharmaceutical compositions, morphic forms and dosage forms thereof.

The invention provides a biomarker for cervical lesion caused by low folic acid mediated HPV infection and application of the biomarker, the biomarker is IGF2BP3 and miR-27a, and the IGF2BP3 and the miR-27a can be used as a therapeutic target and a research tool for cervical lesion caused by low folic acid mediated HPV infection. The small nucleic acid targeting IGF2BP3 and miR-27a is delivered through the folic acid modified exosome in cervical lesion treatment, and the delivery efficiency and the treatment effect are remarkably improved.

The present invention relates to an immunogenic polypeptide comprising (i) a B-cell epitope, (ii) a T-cell epitope, and (iii) a scaffold polypeptide, wherein said scaffold polypeptide is a thioredoxin polypeptide. The present invention further relates to said immunogenic polypeptide for use in medicine and for use in treating and / or preventing inappropriate proliferation of cells and / or infection with an infectious agent, preferably HPV infection, as well as to polypeptides and vectors encoding said immunogenic polypeptide.

The invention refers to the use of PAP-I, a plant ribosome inactivating protein (RIP), or a functional variant thereof for treatment of human papillomavirus (HPV) infection and preventing or treating diseases or conditions associated with HPV infection.

The present application relates to a kind of traditional Chinese medicine for treating female HPV infection, which is prepared from the following weight ratio of traditional Chinese medicine raw materials: 2-4 parts of catechu, 2-4 parts of half lotus, 2-4 parts of snakeberry, 1-2 parts of longjin, 1-2 parts of gotu kola, 1-2 parts of wintergreen, 1-2 parts of coptis, and 1-2 parts of sophora. The present application can effectively treat female HPV infection.

A polynucleotide molecule for preventing or treating HPV infection-related diseases, and an mRNA vaccine, pharmaceutical composition or pharmaceutical product containing the polynucleotide.

The invention belongs to the field of biological medicine, and relates to a traditional Chinese medicine composition for treating high-risk HPV infection. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials: radix sophorae flavescentis, rhizoma smilacis glabrae, fructus cnidii, cyrtomium fortunei, dried alum, pericarpium zanthoxyli, cortex phellodendri and raw radix stemonae.

The invention relates to an external traditional Chinese medicine preparation for preventing and treating HPV (human papillomavirus) infection, which belongs to the technical field of external medicines and comprises the following raw materials: 1-10 parts of gallnut, 1-10 parts of szechwan chinaberry fruit, 1-10 parts of golden cypress, 1-10 parts of fructus cnidii, 1-10 parts of radix sophorae flavescentis, 1-10 parts of cyrtomium rhizome, 1-10 parts of rhizoma atractylodis, 1-10 parts of cortex dictamni, 0-10 parts of calomel, 0-10 parts of cinnabar and 0-10 parts of borneol. The external traditional Chinese medicine preparation disclosed by the invention is locally administrated through the vagina, so that the active ingredients directly reach the focus, and the external traditional Chinese medicine preparation is safe and convenient, and is suitable for resisting HPV infection, cervicitis, cervical erosion, pelvic inflammation, bacterial vaginitis, trichomonas vaginitis, mixed vaginitis, peculiar smell of private parts, pruritus of private parts, dryness of private parts, abnormal leucorrhea and pruritus and causalgia of vulva.

Aspects herein include methods, kits, and systems for evaluating a subject comprising detecting genetic mutation(s) in the DNA sequence of one or more oral cavity squamous cell carcinoma (OCSCC) biomarker(s) in a biological sample from the subject comprising DNA, wherein the OCSCC biomarker(s) comprise TP53, CDKN2A, FAT1, CASP8, NOTCH1, PIK3CA, and / or HRAS along with detecting or determining an HPV infection.

The application belongs to the technical field of disease prevention and treatment, and particularly relates to a formula and a method for preventing and treating HPV infection of female cervical, which specifically comprises 18-22g of Calophyllum inophyllum, 8-12g of Phellodendron, 13-17g of honeysuckle, 8-12g of Sophora, 13-17g of Spermacoce latifolia, 8-12g of Curcuma, 13-17g of Smilax, 13-17g of Cnidium, 13-17g of Isatidis Folium, 8-12g of Sophora, 3-7g of Meligetta, 5-7g of wild chrysanthemum, 2-4g of menthol, 3-5g of sodium hyaluronate, 1-3g of egg yolk globulin powder and distilled water. The formula can effectively prevent and treat HPV infection of female cervical. The formula has the effects of sterilization, removal of corruption, promotion of mycotic regeneration and repair, healing of cervical micro-wounds, and prevention of HPV infection of the body through cervical micro-wounds.

In one aspect, a method for screening an individual for head and neck cancer, which comprising: acquiring a saliva sample from the individual, analyzing the saliva sample to detect one or more of HPVs and at least one of a phenotypic biomarker, a regulatory biomarker, a microbiome-derived biomarker, and a metabolomic biomarker dysregulated due to HPV infection, wherein detection of both of the HPV and at least one of said biomarkers indicates the individual is at risk of head and neck cancer.

The invention discloses application of berberine or berberine hydrochloride in preparation of drugs for resisting HPV infection related diseases. Preferably, the berberine is an extract of natural plants such as coptis chinensis, golden cypress, berberis poiretii schneid or Chinese mahonia. According to the application, the new application of the berberine or the hydrochloride thereof in the aspect of resisting the HPV virus is expanded, and the berberine or the hydrochloride thereof is used for preparing the preparation for resisting the HPV infection, so that diseases related to the HPV infection can be prevented or treated, and the virus is promoted to become negative. As a natural plant extract, berberine is high in safety and not easy to generate drug resistance, and has important clinical significance when being used for preparing Chinese patent medicine products for resisting HPV infection.

This invention discloses a traditional Chinese medicine composition for treating HPV infection, its preparation, and its uses. The composition comprises the following ingredients in the indicated weight percentages: 10-40 parts of *Hedyotis diffusa*, 10-30 parts of *Smilax glabra*, 10-30 parts of *Hedyotis diffusa*, 10-30 parts of *Paeonia suffruticosa*, 10-40 parts of *Lithospermum erythrorhizon*, 10-30 parts of *Dictamnus dasycarpus*, 10-30 parts of *Radix Paeoniae Rubra*, 10-30 parts of *Cicadae Periostracum*, 10-30 parts of *Astragalus membranaceus*, and 10-30 parts of *Glycyrrhiza uralensis*. It effectively inhibits the replication of human papillomavirus (HPV) nucleic acid, thereby effectively treating cervical HPV infection caused by HPV, as well as infectious skin diseases such as cervical precancerous lesions and condyloma acuminata caused by HPV infection.

The invention relates to the field of biological medicine, in particular to a medicine for treating HPV infection related diseases. The invention provides antigen combinations, fusion proteins and encoding nucleic acids. The materials are used for preparing self-replicating RNA vaccines for treating cervical HPV persistent infection, intraepithelial neoplasia induced by the cervical HPV persistent infection and cervical cancer, and indications can be expanded to HPV long-term infected people. Therefore, not only can virus host cells be removed to treat long-term infection, but also precancerous lesions and cervical cancer can be treated more effectively.

The invention discloses application of body resistance strengthening Ermiao detoxification granules in preparation of anti-human papilloma virus drugs, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine is characterized by being prepared from the following components in parts by weight: 30 parts of astragalus membranaceus, 15 parts of codonopsis pilosula, 12 parts of bighead atractylodes rhizome, 15 parts of poria cocos, 9 parts of rhizoma atractylodis, 6 parts of golden cypress, 10 parts of paris polyphylla, 10 parts of rhizoma smilacis glabrae, 10 parts of sculellaria barbata, 10 parts of Chinese lobelia, 15 parts of patrinia, 30 parts of coix seeds, 15 parts of oldenlandia diffusa and 6 parts of liquorice. The number of infected HPV subtypes before treatment, the total score of traditional Chinese medicine syndromes, the TCT result and the pathological result have no significant statistical difference (P is greater than 0.05); after treatment, traditional Chinese medicine syndrome points and clinical index curative effects have a significant statistical difference (Plt; and 0.05), so that the healthy-energy-strengthening ermiao toxin-clearing granules have a remarkable curative effect on high-risk HPV infected patients.

The present application relates to the technical field of HPV repair, in particular to a long-acting sustained-release device combination for treating female vaginal HPV infection and a use method thereof, which provides a safe, low-irritation and convenient-to-use drug delivery mode, and comprises a vaginal drug delivery device A and a device B used in cooperation with each other, the magnetic therapy of the vaginal drug delivery device A can promote capillary dilation and improve microcirculation, the IgY in the drug sustained-release layer of the vaginal drug delivery device A can be stably kept in the gel at a high titer, the alginate sulfate-carboxymethyl chitosan composite microemulsion makes the drug more easily gathered at the infection site, the magnetic therapy and the sustained-release drug interact with each other, promote the absorption and dissipation of inflammation, and produce a good repair environment, the functional powder dressing of the subsequent vaginal drug delivery device B is convenient for mild absorption of the exudate on the surface of the vagina after the magnetic therapy and the drug sustained-release treatment without causing discomfort, and the functional powder dressing releases various ions at a certain rate, further repairing damaged cells and tissues.

The present invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a traditional Chinese medicine composition for anti-HPV virus and its related applications. As a medicine for the treatment and prevention of HPV infection, the traditional Chinese medicine composition takes clearing heat and detoxifying, expelling toxins outwards, removing stasis and promoting new growth as the main treatment principles, and comprises the following raw materials: indigo naturalis, coptis chinensis, polygonum cuspidatum, talc, astragalus membranaceus, liquorice, saxicola undulata, gleditsia sinensis thorn, bletilla striata, chaenomeles sinensis, dragon's blood and borneol. The composition of the present invention has been clinically used for more than 5 years and is found to have a good effect on clearing high-risk HPV infection in the cervix and vagina. The clearance rate can reach more than 80% after 3 to 6 months of clinical local medication, indicating that the traditional Chinese medicine composition has an anti-HPV virus effect. After being wrapped by nanomaterials, the traditional Chinese medicine composition can effectively protect the active molecule while slowly and continuously exerting its efficacy, effectively clearing the cervix and playing an anti-HPV virus role.

The invention discloses Lactobacillus crispatus and its use. The Lactobacillus crispatus is Lactobacillus crispatus chen-01 (Lactobacillus crispatus chen-01), and its deposit number is CGMCC NO.23396. Lactobacillus crispatus can be used to prepare medicines, and the medicines are used to treat and / or prevent HPV infection and vaginal infection. After the medicine prepared by the Lactobacillus crispatus is locally transplanted into the vagina of the present invention, the HPV viral load can be effectively reduced, the HPV clearance rate can be significantly improved, vaginitis can be effectively improved, and the diversity and abundance of vaginal flora can be significantly reduced; in addition, local vaginal transplantation of Lactobacillus crispatus can also significantly improve the vaginal microecological composition, which is close to that of normal healthy people.

The invention relates to an SNP (Single Nucleotide Polymorphism) marker for detecting cervical lesions caused by HPV (Human Papilloma Virus) infection, a kit and application, belongs to the technical field of biological medicines, and aims to provide the SNP marker for detecting the occurrence risk of the cervical lesions caused by HPV infection, and the SNP marker comprises a TLR4 gene SNP site rs11536889. The core of the invention integrates PCR amplification and DNA sequencing technologies, can effectively detect the correlation between the TLR4 gene polymorphism and the cervical lesion occurrence risk, can accurately evaluate the susceptible risk of the cervical lesion, and provides a basis for individualized diagnosis and treatment of clinical medicine.

The present invention relates to a self-sampling kit and method for universally sampling at least one specimen, such as cells, cell residues, DMA, RNA, proteins, viruses, bacteria, parasites or fungi, from the vagina and / or rectal cavity of humans and animals, the kit comprising a self-sampling cloth (10) made of a flexible fabric with an absorbency of 3.5 g / g or less and a sealable receiver (50) for storing and transporting the sampling cloth (10). The present invention also relates to the use of the self-sampling cloth (10) for self-sampling at least one specimen from the body cavity of humans and animals, for example, for diagnosing STIs such as HPV infection.

The invention discloses a nano vaccine for treating HPV (human papilloma virus) infection related cervical lesions as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. In order to solve the problem that the response rate of an existing cervical lesion treatment vaccine does not meet clinical requirements, the hybrid membrane with enhanced immunogenicity and improved safety is prepared by applying a membrane fusion technology and combining an autologous tumor cell membrane and an escherichia coli cytoplasmic membrane; meanwhile, PLGA nano particles loaded with HPV16 E5 / E6 / E7 specific CTL epitope long peptide are used as a delivery carrier, and the HM-NPs-LP nano vaccine co-delivering the hybrid membrane and the HPV16 specific antigen peptide is developed. According to the nano vaccine, tumor cells and HPV16 E5 / E6 / E7 specific CTL epitope long peptide are taken as antigens for immunotherapy, dual immune activation of broad spectrum and specific antigens can be realized, personalized multi-effect treatment capacity for HPV infection related cervical diseases is exerted, targeted killing of HPV infection and pathological cells is enhanced, pathological relapse is prevented, and the nano vaccine has good clinical application prospects. And a new strategy is provided for individualized precise immunotherapy.

The invention discloses application of a traditional Chinese medicine composition for strengthening body resistance, eliminating evil and treating HPV infection, and belongs to the technical field of traditional Chinese medicine, the traditional Chinese medicine composition comprises the following raw materials: 40-50 parts of astragalus membranaceus, 40-50 parts of codonopsis pilosula slices, 40-50 parts of rhizoma smilacis glabrae, 25-45 parts of rehmannia, 10-20 parts of fructus cnidii and 10-20 parts of radix sophorae flavescentis. The traditional Chinese medicine composition is mainly applied in the form of a traditional Chinese medicine external preparation, and can be used for treating one of low-risk HPV infection, high-risk HPV infection and mixed HPV infection and various diseases caused by HPV infection. The traditional Chinese medicines such as astragalus membranaceus, codonopsis pilosula and the like for tonifying healthy qi are matched with the traditional Chinese medicines such as rhizoma smilacis glabrae, fructus cnidii, sophora flavescens, rehmannia glutinosa and the like for clearing away heat and toxic materials and eliminating evil, so that the traditional Chinese medicine composition can be exactly used for the symptoms of qi deficiency and exogenous infection such as HPV (human papillomavirus) infection or qi and blood deficiency caused by interior excess and heat accumulation, and the principle of strengthening healthy qi and eliminating evil is adopted, so that pathogenic factors are attacked without damaging healthy qi, the curative effect can be improved, and disease relapse can be inhibited; and side effects are reduced.

In one aspect, a method for screening an individual for head and neck cancer, which comprising: acquiring a saliva sample from the individual, analyzing the saliva sample to detect one or more of HPVs and at least one of a phenotypic biomarker, a regulatory biomarker, a microbiome-derived biomarker, and a metabolomic biomarker dysregulated due to HPV infection, wherein detection of both of the HPV and at least one of said biomarkers indicates the individual is at risk of head and neck cancer.