The present invention relates to a fluorescent PCR (polymerase chain reaction) detection method for diagnosing low risk type human papilloma virus (HPV) infection, belonging to the in vitro nucleic acid diagnosis field. The present invention comprises a multiple polymerase chain reaction (PCR) system based on fluorescent PCR technique, includes a forward primer and a reverse primer which aim at a plurality of HPV types, as well as a fluorescent probe, and can simultaneously detect DNA comprising six HPV types at most, namely HPV6, 11, 40, 42, 43 and 44, in one reaction tube under proper PCR conditions. The detection method can conveniently and rapidly diagnoss HPV infection in clinical samples, is high in specificity, and has great clinical value for preventing and controlling condyloma acuminatum in an early stage, blocking infection sources and reducing HPV infection.