10 results about "Pcr assay" patented technology

The present invention provides compositions and methods for novel digital PCR (dPCR) assay designs (droplet digital or other systems) for the detection and quantification of multiple Hepatitis B virus (HBV) gene targets. The compositions and methods can further include a non-extendible blocker oligonucleotide to reduce nonspecific inter-amplicon extension.

Described herein are primers, probes, kits, compositions, and methods related to detection of Mycoplasma genitalium (MG) and mutations in quinolone resistance determining region (QRDR) of parC and gyrA.

The present invention relates to compositions and methods for detecting Acinetobacter calcoaceticus-Acinetobacter baumannii complex species and identifying the most common carbapenemases found in carbapenem-resistant Acinetobacter calcoaceticus baumannii (CRAB), such as blaOXA-23-like, blaOXA-24-like, blaOXA-58-like, and blaNDM-like, by multiplex real-time PCR assays.

The present disclosure relates primer pairs, probes, kits, and methods of use thereof to detect virulent strains of Shiga toxin-producing Escherichia coli (E. coli) (STEC).

A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.

Provided are compounds, compositions, kits, systems, devices, and methods for improving an assay such as, for example, a multiplexed PCR assay (e.g., a multiplexed immuno-PCR assay). A solid support (e.g., a bead) may be provided according to some embodiments of the present invention. The solid support may comprise an encoding agent (e.g., a dye), a nucleic acid sequence (e.g., an oligonucleotide and / or primer); and a molecular recognition element (e.g., an antibody). A detection reagent may be provided according to some embodiments of the present invention. The detection reagent may comprise a molecular recognition element (e.g., an antibody) and a nucleic acid tag. In some embodiments, at least a portion of the nucleic acid sequence of the solid support and at least a portion of the nucleic acid target tag of the detection reagent are configured to participate in a nucleic acid amplification process. A solid support and detection reagent may bind to the same target, thereby forming a reagent pair.

The present invention relates to a method of certifying a test animal-derived product sample, the method comprising the steps of:(a) determining a test methylation profile of one or more pre-selected methylation sites within the genomic material obtained from the test animal derived product sample; and(b) comparing the test methylation profile obtained from (a) with a reference methylation profile obtained from a control animal of the same biological taxon of the test animal from which the product sample is derived from, where the control animal is bred under a known distinct type of animal husbandry;wherein a significant similarity in the test methylation profile of (a) compared to the reference methylation profile from the control animal, is indicative of the test animal having been bred under the same distinct type of animal husbandry as the control animal and the test animal-derived product is certified so;wherein a difference in the test methylation profile of (a) compared to the reference methylation profile of the control animal, is indicative of the test animal having been bred under another distinct type of animal husbandry as the control animal; andwherein the pre-selected methylation sites are CpG sites selected from genes or regions of genomic DNA from the control and test animal that show the highest degree of methylation variation during the training of the method; andwherein the test methylation profile has significant similarity to the reference methylation profile when the test methylation profile overlaps with the reference methylation profile that is defined by multiple training samples using Principal Component analysis and / or Multi-Dimensional Scaling; andwherein the methylation profiles are determined using at least one method selected from the group consisting of PCR, methylation-specific PCR, real-time methylation-specific PCR, PCR assay using a methylation DNA-specific binding protein, quantitative PCR, a DNA chip-based assay, pyrosequencing, bisulfate pyrosequencing, Methylated DNA immunoprecipitation-sequencing and combinations thereof; andwherein the test animal is selected from livestock or poultry.

The present invention relates to compositions and methods to detect Group B Streptococcus (GBS, Streptococcus agalactiae) and to identify the most prevalent genes responsible for clindamycin resistant GBS such as ermB, ermTR, ermT, lsaC and lsaE by a multiplex real-time PCR assay.

An antibody-antigen capture ELISA-PCR test set for detection of a target protein comprises a first antibody-DNA conjugate having a first antibody which is configured to bind to a target protein; and a first DNA oligo linked to the first antibody; a second antibody-DNA conjugate having a second antibody which is configured to bind to the target protein; and a second DNA oligo linked to the second antibody; and multiple immobilized DNA fragments attached to a designated surface on their attaching ends, wherein the immobilized DNA fragments are complementary to one of the first DNA oligo and the second DNA oligo.

Provided are compounds, compositions, kits, systems, devices, and methods for improving an assay such as, for example, a multiplexed PCR assay (e.g., a multiplexed immuno-PCR assay). A solid support (e.g., a bead) may be provided according to some embodiments of the present invention. The solid support may comprise an encoding agent (e.g., a dye), a nucleic acid sequence (e.g., an oligonucleotide and / or primer); and a molecular recognition element (e.g., an antibody). A detection reagent may be provided according to some embodiments of the present invention. The detection reagent may comprise a molecular recognition element (e.g., an antibody) and a nucleic acid tag. In some embodiments, at least a portion of the nucleic acid sequence of the solid support and at least a portion of the nucleic acid tag of the detection reagent are configured to participate in a nucleic acid amplification process. A solid support and detection reagent may bind to the same target, thereby forming a reagent pair.