255 results about "Molecular recognition" patented technology

The invention relates to the technical field of analysis and detection, in particular to a molecularly imprinted sensor for in-situ detection of epicatechin in plant leaves and a preparation and application method of the molecularly imprinted sensor. The molecular imprinting sensor for detecting epicatechin is prepared by assembling a zeolite imidazate skeleton-amide functionalized single-walled carbon nanotube composite material, amino-formyl imidazole modified reduced graphene and an MIP film on the surface of an electrode layer by layer. The high-selectivity and high-sensitivity detection on the target molecule epicatechin is realized. The technology can effectively reduce the detection cost, simplify the operation process and improve the performance of the sensor. Particularly, due to the introduction of the MIP, the sensor has the capabilities of in-situ detection and high-precision molecular recognition, can realize rapid and reliable quantitative analysis in a complex sample, and has a wide application prospect.

The invention discloses a nanometer delivery device for a gastric cancer targeted drug and a use method of the nanometer delivery device, and belongs to the technical field of drug targeted delivery. The device comprises a molecular recognition and optimization module which is responsible for accurately recognizing a tumor cell specific molecular marker and providing personalized treatment strategy recommendation; the nano-carrier design module is used for designing multifunctional lipid-polymer hybrid nano-particles and carrying out surface targeting ligand modification; the intelligent drug loading module is used for improving the stability and controllability of drug loading based on a pH / temperature sensitive release mechanism and effectively reducing the toxic influence of non-targeted tissues; the molecular imaging and monitoring module integrates a nanoprobe imaging technology and a quantum dot tracing technology, constructs a treatment-imaging integrated system, realizes real-time dynamic monitoring of a treatment process, and provides instant feedback of a molecular level; and the risk assessment module is used for establishing a safety database and providing dynamic risk assessment of the treatment process through a biological safety assessment system and long-term tracking management.

The invention relates to a target molecule identification and quantification method based on a solid nanopore detection technology, electronic equipment and a computer readable storage medium. The method comprises the following steps: (1) acquiring current signal data generated by a target molecule through a nanopore sensor within a period of time; (2) carrying out filtering processing on the current signal data; (3) identifying the filtered data to obtain a target via hole event set; (4) filtering the target via hole event set, and extracting signal data of the filtered events to obtain a target molecule recognition event; and (5) calculating the average interval time of occurrence of the target molecule recognition event, and obtaining the occurrence frequency of the target molecule based on the average interval time, namely the relative concentration of the target molecule. According to the method, automatic identification and quantitative analysis are realized aiming at mass signal data generated by a solid-state nanopore detection technology, and the analysis efficiency and accuracy are improved by optimizing a data identification method.

The invention discloses a preparation method and application of a glucose sensor based on an acupuncture needle, the sensor is prepared based on a specific structural design and a molecular recognition mechanism by taking the acupuncture needle as a substrate, and high-specificity and high-sensitivity detection of glucose concentration is realized through drain current change. According to the present invention, the biosensor has the ultra-wide dynamic detection range (0-40 mmol.L <-1 >), covers the clinical range from hypoglycemia to severe hyperglycemia, has the low detection limit of 54 mol.L <-1 >, has excellent grid regulation and control ability, shows the significant on-off ratio and the rapid response characteristic during the detection process, and meets the requirements of the biosensor on sensitivity and real-time performance; and the device has quick response capability to grid voltage change, so that the device has good stability and repeatability under repeated operation, and a direction and a basis can be provided for the development of a blood glucose monitoring technology with continuous, real-time and accurate characteristics.

The invention discloses a gradient frame membrane and separation application thereof. An integrated gradient membrane material is constructed in a mode of functional group sharing, metal node sharing or skeleton interpenetration, and target molecule recognition sites are designed by utilizing the advantage that a framework material is easy to modify, so that the separation performance is remarkably improved. The gradient film structure comprises a metal organic-metal organic framework gradient film, a metal organic-covalent organic framework gradient film and a covalent organic-metal organic framework gradient film. The invention introduces a new structure of a metal organic framework-covalent organic framework gradient film and a construction strategy thereof in detail. The preparation method comprises the steps of preparing a bottom layer film structure from bottom to top, then preparing a top layer film structure on the bottom layer film structure, performing post-modification and the like. By designing a specific asymmetric porous structure and adsorption sites, the membrane shows excellent ethylene / ethane, propylene / propane, ion screening and organic matter dehydration separation performance under the dual action of adsorption selection and shape-selective screening.

The invention discloses an SERS (Surface Enhanced Raman Scattering) substrate with a needle-like diagram nanostructure and a preparation method of the SERS substrate, and belongs to the field of nanotechnology and detection. The preparation method of the needle-like Turing nano-structure SERS substrate comprises the following steps: by taking a silver needle as a carrier, carrying out electrochemical etching on the surface of the silver needle to induce to form a Turing nano-structure with fractal topological characteristics, and then inducing to conformally grow a layer of zeolite imidazole framework-8 material as a shell layer by utilizing the nano curvature characteristics of the fractal structure, so as to prepare the needle-like Turing nano-structure SERS substrate. And a composite SERS enhanced platform with an electromagnetic field hot spot aggregation effect and a molecular screening and enriching capability is constructed. The platform has the advantages of high structural uniformity, expandability in manufacturing, synergy of signal enhancement and molecular recognition and the like, remarkably improves the detection sensitivity and the signal reproducibility, and is suitable for trace analysis in a complex sample system, such as related application in the fields of food safety, environmental pollution monitoring, biomedicine and the like.

The invention discloses a method for analyzing a complex system by using a surface enhanced Raman spectrum three-dimensional code and application of the method. The method specifically comprises the following steps: obtaining SERS sensing channels with different affinity characteristics by using an unmarked or functionally-marked SERS substrate; collecting an SERS spectrum data set by utilizing the constructed SERS sensing channel, and integrating the SERS spectrum data set into a'three-dimensional code 'image through data conversion and integration of three dimensions of Raman vibration band distribution, the SERS sensing channel and SERS intensity, so as to realize imaging of the molecular landscape; the feature information in the SERS three-dimensional code is screened and extracted through a convolutional neural network algorithm, and a mapping relation between the SERS three-dimensional code and a complex system label is established, so that a complex system classification / regression model is established, rapid qualitative or quantitative analysis of a complex system is realized, and the accuracy of the SERS three-dimensional code is improved. More applications can be expanded in the fields of molecular recognition, biological sample classification and identification, disease diagnosis, cell monitoring and the like.

Systems and methods for determining a molecular structure include obtaining mass spectrum data for an unknown parent ion. The mass spectrum data is recorded at each collision energy of a series of collision energies and includes an abundance of one or more substructures of the unknown parent ion. The molecular structure of the unknown parent ion is determined by composing the mass spectrum data into a fragmentation profile and embedding the fragmentation profile into a reduced dimension profile. The reduced dimension profile is mapped into a fragmentation space. One or more proximate molecules in the fragmentation space are identified using the mapping and a candidate for the parent ion is identified using the one or more proximate molecules.

Disclosed herein is a composite material comprising:a substrate; anda patterned hydrogel disposed on the substrate, wherein:the patterned hydrogel comprises stimulus-responsive constitutional units and constitutional units comprising one or more target molecule recognition moieties;the stimulus-responsive constitutional units are responsive to a stimulus and are configured to produce a stress value S1 to the patterned hydrogel when the stimulus is applied;the target molecule recognition moieties are responsive to a target molecule and are configured to impart a stress value S2 to the patterned hydrogel upon interaction with the target molecule;the patterned hydrogel is configured to reversibly buckle and / or reversibly swell when a threshold stress level T of the patterned hydrogel is crossed.

The invention discloses a hollow covalent organic framework fiber separation membrane with a molecular recognition function and a preparation method and application thereof.The method comprises the steps that polylactic acid and aldehyde monomers are added into a solvent A, and an aldehyde-loaded spinning solution is prepared; spinning the aldehyde-loaded spinning solution to obtain an aldehyde fiber precursor membrane; taking the formylated fiber precursor membrane as a template, immersing the formylated fiber precursor membrane into a reaction system consisting of an amino monomer, a catalyst and a solvent B, and initiating dynamic covalent assembly to prepare a COF shell-coated fiber membrane; immersing the fiber membrane coated with the COF shell layer into a solvent C to remove the polylactic acid template so as to prepare hollow COF fibers; and immersing the hollow COF fiber into a solvent system containing a gas recognition functional monomer, and constructing a gas molecule recognition layer on the surface of the hollow COF fiber to prepare the hollow COF fiber separation membrane. According to the application, the hollow covalent organic framework fiber separation membrane is applied to precise recognition operation of sulfur-containing gas. According to the invention, an efficient and energy-saving innovative membrane material with resource enrichment potential can be prepared.

The invention relates to the technical field of metal organic framework materials (MOF) and isotope separation, in particular to a functionalized metal organic framework material for water / heavy water separation and a preparation method and application thereof. The metal organic framework material is a porous crystal material which is formed by coordination self-assembly of metal ions and V-shaped organic ligands and has a periodic network structure; the V-shaped organic ligand is a V-shaped dicarboxylic acid organic ligand modified by an oxygen-containing functional group designed through topological functionalization. According to the metal organic framework material, through topological functional design of a V-shaped organic ligand, a space confinement channel and a high-density oxygen active site are integrated in the same MOF material, and an ideal model system is provided for identification of water isotopes; according to the metal organic framework material, the framework structure flexibility, the pore channel confinement effect and the molecular recognition capability can be synergistically regulated and controlled, so that efficient recognition and separation of water / heavy water are realized.

The invention belongs to the technical field of target sample characterization, and provides application of enterococcus pore-forming toxin mutant protein in nanopore detection. The enterococcus pore-forming toxin mutant protein is EPX1 nanopore mutant protein and is obtained by mutating wild type EPX1 nanopore protein, the amino acid sequence of the wild type EPX1 nanopore protein is as shown in SEQ ID NO: 1, and the mutation means that one or more amino acids of the wild type EPX1 nanopore protein are mutated into common amino acids except original amino acids. The EPX1 protein (wild type) is a type of pore-forming toxin protein and widely exists in various living bodies in nature, and a mutant of the EPX1 protein shows excellent pore-forming capacity after being modified (after amino acid mutation). The structural stability, the molecular recognition efficiency and the detection performance of the EPX1 nanopore mutant protein are remarkably improved.

The invention discloses an intelligent dual-mode substance detection method based on a divalent nucleic acid aptamer, which comprises a fluorescence detection mode and a colorimetric detection mode, and belongs to the technical field of analytical chemistry. The divalent aptamer with a double-hairpin structure is complementary with a complementary sequence of the divalent aptamer, and under the action of sodium borohydride and silver nitrate, the divalent aptamer and the complementary sequence of the divalent aptamer are subjected to fluorescence detection and colorimetric detection to obtain the intelligent dual-mode substance detection method based on the divalent nucleic acid aptamer. And respectively forming the DNA silver nano-cluster and the DNA adsorbed silver nano-particles at different time and at different temperatures. In the two detection modes, the divalent aptamers are molecular recognizers, the complementary sequences of the divalent aptamers are signal enhancement probes, rapid and ultra-sensitive detection is achieved by detecting fluorescence and absorbance and analyzing R + B values of colorimetric images through a smartphone APP, the detection method is easy and convenient to operate, efficient and sensitive, the recovery rate and the anti-interference capacity are good in labeling actual sample detection, and the detection method is suitable for large-scale popularization and application. The method has a good application prospect in qualitative and quantitative analysis of aminoglycoside antibiotic compounds.

The invention provides a broadband terahertz fingerprint detection sensor based on a gradient metasurface, and relates to specific molecular recognition and detection. The gradient metasurface array is integrated on the upper surface of the substrate; the gradient metasurface array comprises a plurality of micro antenna unit structures, and each micro antenna unit structure specifically comprises an equal-length metal microrod array and a gradient metal microrod array; the isometric metal micro-rod array comprises a plurality of isometric metal micro-rods which are arranged in parallel; the gradient metal micro-rod array comprises a plurality of gold micro-rods which are arranged in parallel and are gradually changed in length; and the plurality of metal micro-rods with gradually reduced lengths are inserted into the gaps formed by the plurality of metal micro-rods with equal lengths. The gradient metasurface provided by the invention shows extraordinary resonance density in a limited and small printing area, the metasurface which is operated on a wide spectrum at the same time is realized along with the increase of the complexity of a sample, abundant spectrum information can be obtained on the wide spectrum, and tiny differences in fingerprints can be analyzed and absorbed through narrow-band resonance.

The invention is applicable to the technical field of metal organic framework materials, and provides a pillararene-based metal organic framework material and a preparation method thereof.The pillararene-based metal organic framework material takes a compound PY2-P5 as a ligand, and the material is one of PMOF1, PMOF2 and PMOF3; the PMOF1 is of a Cu4I4 cubic tetramer structure; the PMOF2 is of a Cu4I4 ladder tetramer structure, and the Cu4I4 ladder tetramer structure is a And the PMOF3 has a CuI one-dimensional chain structure. By introducing the pillararene ligand and regulating and controlling the coordination mode of the pillararene ligand and cuprous iodide, the metal organic framework material with stable structure and functional characteristics is constructed, the metal organic framework material has the dual advantages of host recognition site and fluorescence signal response, and can selectively distinguish 1-bromoalkane and 2-bromoalkane; a remarkable fluorescent blue shift signal is shown in the adsorption process, so that the visualization of molecular recognition and sensing is realized; the excellent molecular recognition capability of pillararene is maintained, the stability and reusability of the material are improved through framing, and a new way is provided for selective detection and separation of volatile organic compounds.

To provide a gene testing method and a gene testing kit for analyzing a variable region of a TCR or a BCR in single-cell analysis.SOLUTION: A method including a step of capturing mRNA eluted from a single cell using an RT probe immobilized on a solid-phase surface and including a common sequence, a cell identification barcode, a first molecular identification barcode, and an oligo(dT) sequence, a step of performing a reverse transcription reaction in a reverse transcription reaction solution containing a TSO using the mRNA as a template to generate cDNA having a sequence added to a 3' end, a step of degrading the mRNA, a step in which a primer for inserting a second molecular identification barcode binds to a constant region adjacent to a variable region of a TCR or a BCR and includes the second molecular identification barcode, a step of ligating cDNA extended from the primer with a ligase, a step of amplifying a fragment including the second molecular identification barcode and the variable region of the TCR or the BCR using a primer, a step of performing sequence analysis, and a step of associating sequences.SELECTED DRAWING: Figure 2

Provided herein are compositions and methods for the multiplexed profiling of RNA and DNA modifications across transcriptomes and genomes, respectively. The methods combine molecular recognition of non-canonical features (e.g., base modifications, backbone modifications, lesions, and / or structural elements) of a target nucleic acid with a step of writing the information from this recognition event into the neighboring genetic sequence of the target nucleic acid using a barcode. The resultant barcoded nucleic acids are then converted into sequencing libraries and read by DNA / RNA sequencing methods. This step reveals the sequence of the barcode, which is correlated with the non-canonical feature in the target nucleic acid(s). The high throughput profiling methods described herein allow for localization of one or more modifications in a target nucleic acid. The methods also allow for identification of the nature and location of several or all DNA / RNA modifications in parallel.

The invention discloses a naphthoquinone-based gas sensitive material, a preparation method and application of the naphthoquinone-based gas sensitive material in an ammonia gas sensor, and belongs to the technical field of gas sensors. The material is composed of divalent metal ions such as copper, nickel and cobalt and a polysubstituted naphthoquinone organic ligand, forms a one-dimensional chain structure, has a high specific surface area, an ordered electron channel and excellent molecular recognition capability, and can detect toxic gases such as ammonia gas at room temperature with high sensitivity. Macroscopic preparation of the material can be realized through solvothermal one-step reaction. Compared with the prior art, the one-dimensional coordination structure and ligand site engineering synergistically improve the carrier transport and molecular recognition capability, can realize continuous, real-time and selective detection of ammonia gas under complex working conditions, and has the advantages of high yield, large-scale preparation and excellent device application prospect.

The application discloses a fluorine removal agent and a preparation method thereof, and relates to the technical field of fluorine removal agents, and comprises the following components in parts by weight: 5-10 parts of an aluminum salt, 5-10 parts of an iron salt, 15-20 parts of a rare earth material, sodium citrate and beta-cyclodextrin; the addition amount of the sodium citrate is 5%-10% of the total mass of the aluminum salt, the iron salt and the rare earth material; and the addition amount of the beta-cyclodextrin is 30%-50% of the mass of the sodium citrate. Through the introduction of the synergistic system of sodium citrate and beta-cyclodextrin, the metal ion precipitation sequence is regulated, meanwhile, the beta-cyclodextrin captures competitive anions through molecular recognition and constructs a hydrogen bond network to block interference, and the combination of the microsphere type and the porous particle type further realizes dynamic directional transmission of pollutants and nuclear pore limited crystallization, and the active site distribution is reconstructed at the molecular level, so that a technical scheme with universality, economy and reliability is provided for the treatment of complex fluorine-containing wastewater.

The invention relates to the technical field of spectroscopy and molecular recognition, in particular to an SERS (Surface Enhanced Raman Scattering)-based technology. The invention provides an SERS (Surface Enhanced Raman Scattering) substrate for detecting various biomass molecules, which is characterized in that a chain polymer is used as a soft template, and a metal monatomic head-to-head local self-assembly structure is formed on the surface of a carrier. The structure not only promotes mutual proximity between metal single atoms, but also physically blocks aggregation of the metal atoms to the cluster structure in a manner of passivating the surface of the carrier. Charge redistribution between adjacent metal single atoms regulates and controls the electronic state of the surface of the whole semiconductor substrate, and then efficient photoinduced charge transfer is induced, so that low-frequency Raman signals of biomass molecules are amplified.

The invention discloses a fluorescent probe for beta-lactoglobulin detection and a preparation method and application thereof, and relates to the technical field of food detection. The structure of the fluorescent probe is shown in the specification, r1 and R2 are independently selected from hydrogen or C1-C5 alkoxy, and R3 is methyl or ethyl. Aggregation-induced emission (AIE) is combined with inhibition of twisted intramolecular charge transfer (TICT) when a beta-lactoglobulin (beta-LG) cavity is inserted, a D-pi-A skeleton is adopted, triphenylamine is used as a conservative electron donor and a conservative molecular rotor, and cavity coordination and static electricity are optimized by repositioning receptor connection, so that ICT and charge distribution are systematically adjusted. The cavity locking strategy integrates molecular recognition and motion limitation, and can realize rapid analysis and specific detection of beta-LG in a complex dairy product matrix.

The invention relates to the technical field of separation membranes, in particular to a self-assembled ion separation membrane based on a pillararene derivative and a preparation method of the self-assembled ion separation membrane. The pillararene derivative and the triaminoguanidine hydrochloride are respectively dissolved, so that the problems of instant precipitation and phase separation easily caused by direct mixing are effectively avoided. Meanwhile, two functional monomers are ultrasonically dispersed, so that the problem of molecular agglomeration in the mixing process is avoided, the uniformity of reaction liquid is ensured, and a foundation is laid for subsequently forming a flawless separation membrane. The pillararene derivative contained in the separation membrane structure provides a macrocyclic pore structure with ion recognition capability, and triaminoguanidine hydrochloride acts on sites through multiple hydrogen bonds / ionic bonds, so that the selective separation performance of the membrane is further enhanced. Due to the collaborative design of the material, the material has precise molecular recognition capability and adjustable aperture, and can realize efficient selective separation of target objects such as small organic molecules, ions or dyes.

The present invention discloses a method for cyclodextrin molecular recognition based on nuclear magnetic resonance. The method comprises: modifying cyclodextrin with imidazole groups, using nuclear magnetic resonance to identify guest molecules with carboxyl or sulfonic acid groups using the modified cyclodextrin, and obtaining nuclear magnetic resonance spectra before and after cyclodextrin inclusion. If the peak of the monomer in the mixture spectrum shows a chemical shift and peak shape change compared to the spectrum of the cyclodextrin, it indicates that there is an interaction between the host and the guest. The present invention provides a method for the specific recognition of cyclodextrin by guest molecules based on nuclear magnetic resonance detection. The method is simple to operate and has good recognition effect, which is of great significance for the development of new molecular recognition systems.

The present disclosure relates to methods and kits for analyzing a macromolecule. In some embodiments, the present disclosure relates to macromolecule analysis methods which employ barcoding and nucleic acid encoding of molecular recognition events. Also provided herein is a method and related kits for transferring information using a plurality of enzymes, including for performing a ligation, extension, and cleavage reaction with nucleic acid molecules associated with the macromolecule for analysis. In some embodiments, the macromolecule for analysis comprises a peptide, a polypeptide, or a protein.

The application belongs to the technical field of organic chemical synthesis, and particularly relates to a nitrogen center chiral base derivative, a preparation method and application thereof. The preparation method of the nitrogen center chiral base derivative provided by the application can effectively synthesize a base with only a nitrogen chiral center by using a tetrahydrodibenzo-diazocine compound as a raw material and through chiral phosphoric acid catalyzed amination reaction. Moreover, the synthesis method provided by the application has the advantages of high efficiency, simple steps and strong enantioselectivity, is suitable for industrialized production of chiral bases and derivatives thereof, and provides a new approach for research and application of chiral compounds. Meanwhile, the base derivative prepared by the application is a base molecule with a nitrogen center chirality, and has wide application prospects in the fields of molecular recognition, DNA binding, supramolecular chemistry, material science, asymmetric catalysis and drug development.

The invention belongs to the field of coordination polymers, and discloses an antiferromagnetic tetranuclear cobalt coordination polymer constructed by mixed polydentate ligands and a preparation method and application thereof. The chemical formula of the polymer is (C9H9O6) 2 (C26H18N4) 2 (OH) 2Co4. (H2O) 7, C9H9O6 is 1, 3, 5-cyclohexane tricarboxylic acid, C26H18N4 is 1, 2, 4, 5-tetrapyridyl benzene, the polymer is a monoclinic system and a P21 / c space group, the cell parameters are alpha = 90 degrees, beta = 96.166 (8) degrees and gamma = 90 degrees, and the polymer has few defects, high crystallinity and good stability and can be applied to the fields of adsorption separation, hydrogen storage, energy storage, molecular magnets, catalysis, sensing or molecular recognition and the like.

The present invention discloses a dual-mode intelligent substance detection method based on bivalent nucleic acid aptamers, which includes two detection modes: fluorescence and colorimetry. This method belongs to the field of analytical chemistry. The present invention combines a bivalent aptamer with a double hairpin structure with its complementary sequence. Under the action of sodium borohydride and silver nitrate, DNA-silver nanoclusters and DNA-adsorbed silver nanoparticles are formed at different times and temperatures, respectively. In both detection modes, the bivalent aptamer acts as a molecular recognition target, and its complementary sequence acts as a signal-enhancing probe. Rapid, ultrasensitive detection is achieved by detecting fluorescence, absorbance, and analyzing the R+B value of colorimetric images using a smartphone app. This detection method is simple to operate, efficient, and sensitive, and exhibits good recovery and anti-interference capabilities in spiked sample testing. It has promising application prospects in the qualitative and quantitative analysis of aminoglycoside antibiotic compounds.