163 results about "Molecular recognition" patented technology

The invention relates to a target molecule identification and quantification method based on a solid nanopore detection technology, electronic equipment and a computer readable storage medium. The method comprises the following steps: (1) acquiring current signal data generated by a target molecule through a nanopore sensor within a period of time; (2) carrying out filtering processing on the current signal data; (3) identifying the filtered data to obtain a target via hole event set; (4) filtering the target via hole event set, and extracting signal data of the filtered events to obtain a target molecule recognition event; and (5) calculating the average interval time of occurrence of the target molecule recognition event, and obtaining the occurrence frequency of the target molecule based on the average interval time, namely the relative concentration of the target molecule. According to the method, automatic identification and quantitative analysis are realized aiming at mass signal data generated by a solid-state nanopore detection technology, and the analysis efficiency and accuracy are improved by optimizing a data identification method.

The invention discloses a gradient frame membrane and separation application thereof. An integrated gradient membrane material is constructed in a mode of functional group sharing, metal node sharing or skeleton interpenetration, and target molecule recognition sites are designed by utilizing the advantage that a framework material is easy to modify, so that the separation performance is remarkably improved. The gradient film structure comprises a metal organic-metal organic framework gradient film, a metal organic-covalent organic framework gradient film and a covalent organic-metal organic framework gradient film. The invention introduces a new structure of a metal organic framework-covalent organic framework gradient film and a construction strategy thereof in detail. The preparation method comprises the steps of preparing a bottom layer film structure from bottom to top, then preparing a top layer film structure on the bottom layer film structure, performing post-modification and the like. By designing a specific asymmetric porous structure and adsorption sites, the membrane shows excellent ethylene / ethane, propylene / propane, ion screening and organic matter dehydration separation performance under the dual action of adsorption selection and shape-selective screening.

The invention discloses a hollow covalent organic framework fiber separation membrane with a molecular recognition function and a preparation method and application thereof.The method comprises the steps that polylactic acid and aldehyde monomers are added into a solvent A, and an aldehyde-loaded spinning solution is prepared; spinning the aldehyde-loaded spinning solution to obtain an aldehyde fiber precursor membrane; taking the formylated fiber precursor membrane as a template, immersing the formylated fiber precursor membrane into a reaction system consisting of an amino monomer, a catalyst and a solvent B, and initiating dynamic covalent assembly to prepare a COF shell-coated fiber membrane; immersing the fiber membrane coated with the COF shell layer into a solvent C to remove the polylactic acid template so as to prepare hollow COF fibers; and immersing the hollow COF fiber into a solvent system containing a gas recognition functional monomer, and constructing a gas molecule recognition layer on the surface of the hollow COF fiber to prepare the hollow COF fiber separation membrane. According to the application, the hollow covalent organic framework fiber separation membrane is applied to precise recognition operation of sulfur-containing gas. According to the invention, an efficient and energy-saving innovative membrane material with resource enrichment potential can be prepared.

The invention relates to the technical field of metal organic framework materials (MOF) and isotope separation, in particular to a functionalized metal organic framework material for water / heavy water separation and a preparation method and application thereof. The metal organic framework material is a porous crystal material which is formed by coordination self-assembly of metal ions and V-shaped organic ligands and has a periodic network structure; the V-shaped organic ligand is a V-shaped dicarboxylic acid organic ligand modified by an oxygen-containing functional group designed through topological functionalization. According to the metal organic framework material, through topological functional design of a V-shaped organic ligand, a space confinement channel and a high-density oxygen active site are integrated in the same MOF material, and an ideal model system is provided for identification of water isotopes; according to the metal organic framework material, the framework structure flexibility, the pore channel confinement effect and the molecular recognition capability can be synergistically regulated and controlled, so that efficient recognition and separation of water / heavy water are realized.

The invention belongs to the technical field of target sample characterization, and provides application of enterococcus pore-forming toxin mutant protein in nanopore detection. The enterococcus pore-forming toxin mutant protein is EPX1 nanopore mutant protein and is obtained by mutating wild type EPX1 nanopore protein, the amino acid sequence of the wild type EPX1 nanopore protein is as shown in SEQ ID NO: 1, and the mutation means that one or more amino acids of the wild type EPX1 nanopore protein are mutated into common amino acids except original amino acids. The EPX1 protein (wild type) is a type of pore-forming toxin protein and widely exists in various living bodies in nature, and a mutant of the EPX1 protein shows excellent pore-forming capacity after being modified (after amino acid mutation). The structural stability, the molecular recognition efficiency and the detection performance of the EPX1 nanopore mutant protein are remarkably improved.

The invention is applicable to the technical field of metal organic framework materials, and provides a pillararene-based metal organic framework material and a preparation method thereof.The pillararene-based metal organic framework material takes a compound PY2-P5 as a ligand, and the material is one of PMOF1, PMOF2 and PMOF3; the PMOF1 is of a Cu4I4 cubic tetramer structure; the PMOF2 is of a Cu4I4 ladder tetramer structure, and the Cu4I4 ladder tetramer structure is a And the PMOF3 has a CuI one-dimensional chain structure. By introducing the pillararene ligand and regulating and controlling the coordination mode of the pillararene ligand and cuprous iodide, the metal organic framework material with stable structure and functional characteristics is constructed, the metal organic framework material has the dual advantages of host recognition site and fluorescence signal response, and can selectively distinguish 1-bromoalkane and 2-bromoalkane; a remarkable fluorescent blue shift signal is shown in the adsorption process, so that the visualization of molecular recognition and sensing is realized; the excellent molecular recognition capability of pillararene is maintained, the stability and reusability of the material are improved through framing, and a new way is provided for selective detection and separation of volatile organic compounds.

To provide a gene testing method and a gene testing kit for analyzing a variable region of a TCR or a BCR in single-cell analysis.SOLUTION: A method including a step of capturing mRNA eluted from a single cell using an RT probe immobilized on a solid-phase surface and including a common sequence, a cell identification barcode, a first molecular identification barcode, and an oligo(dT) sequence, a step of performing a reverse transcription reaction in a reverse transcription reaction solution containing a TSO using the mRNA as a template to generate cDNA having a sequence added to a 3' end, a step of degrading the mRNA, a step in which a primer for inserting a second molecular identification barcode binds to a constant region adjacent to a variable region of a TCR or a BCR and includes the second molecular identification barcode, a step of ligating cDNA extended from the primer with a ligase, a step of amplifying a fragment including the second molecular identification barcode and the variable region of the TCR or the BCR using a primer, a step of performing sequence analysis, and a step of associating sequences.SELECTED DRAWING: Figure 2

Provided herein are compositions and methods for the multiplexed profiling of RNA and DNA modifications across transcriptomes and genomes, respectively. The methods combine molecular recognition of non-canonical features (e.g., base modifications, backbone modifications, lesions, and / or structural elements) of a target nucleic acid with a step of writing the information from this recognition event into the neighboring genetic sequence of the target nucleic acid using a barcode. The resultant barcoded nucleic acids are then converted into sequencing libraries and read by DNA / RNA sequencing methods. This step reveals the sequence of the barcode, which is correlated with the non-canonical feature in the target nucleic acid(s). The high throughput profiling methods described herein allow for localization of one or more modifications in a target nucleic acid. The methods also allow for identification of the nature and location of several or all DNA / RNA modifications in parallel.

The invention discloses a naphthoquinone-based gas sensitive material, a preparation method and application of the naphthoquinone-based gas sensitive material in an ammonia gas sensor, and belongs to the technical field of gas sensors. The material is composed of divalent metal ions such as copper, nickel and cobalt and a polysubstituted naphthoquinone organic ligand, forms a one-dimensional chain structure, has a high specific surface area, an ordered electron channel and excellent molecular recognition capability, and can detect toxic gases such as ammonia gas at room temperature with high sensitivity. Macroscopic preparation of the material can be realized through solvothermal one-step reaction. Compared with the prior art, the one-dimensional coordination structure and ligand site engineering synergistically improve the carrier transport and molecular recognition capability, can realize continuous, real-time and selective detection of ammonia gas under complex working conditions, and has the advantages of high yield, large-scale preparation and excellent device application prospect.

The application discloses a fluorine removal agent and a preparation method thereof, and relates to the technical field of fluorine removal agents, and comprises the following components in parts by weight: 5-10 parts of an aluminum salt, 5-10 parts of an iron salt, 15-20 parts of a rare earth material, sodium citrate and beta-cyclodextrin; the addition amount of the sodium citrate is 5%-10% of the total mass of the aluminum salt, the iron salt and the rare earth material; and the addition amount of the beta-cyclodextrin is 30%-50% of the mass of the sodium citrate. Through the introduction of the synergistic system of sodium citrate and beta-cyclodextrin, the metal ion precipitation sequence is regulated, meanwhile, the beta-cyclodextrin captures competitive anions through molecular recognition and constructs a hydrogen bond network to block interference, and the combination of the microsphere type and the porous particle type further realizes dynamic directional transmission of pollutants and nuclear pore limited crystallization, and the active site distribution is reconstructed at the molecular level, so that a technical scheme with universality, economy and reliability is provided for the treatment of complex fluorine-containing wastewater.

The invention discloses a fluorescent probe for beta-lactoglobulin detection and a preparation method and application thereof, and relates to the technical field of food detection. The structure of the fluorescent probe is shown in the specification, r1 and R2 are independently selected from hydrogen or C1-C5 alkoxy, and R3 is methyl or ethyl. Aggregation-induced emission (AIE) is combined with inhibition of twisted intramolecular charge transfer (TICT) when a beta-lactoglobulin (beta-LG) cavity is inserted, a D-pi-A skeleton is adopted, triphenylamine is used as a conservative electron donor and a conservative molecular rotor, and cavity coordination and static electricity are optimized by repositioning receptor connection, so that ICT and charge distribution are systematically adjusted. The cavity locking strategy integrates molecular recognition and motion limitation, and can realize rapid analysis and specific detection of beta-LG in a complex dairy product matrix.

The invention relates to the technical field of separation membranes, in particular to a self-assembled ion separation membrane based on a pillararene derivative and a preparation method of the self-assembled ion separation membrane. The pillararene derivative and the triaminoguanidine hydrochloride are respectively dissolved, so that the problems of instant precipitation and phase separation easily caused by direct mixing are effectively avoided. Meanwhile, two functional monomers are ultrasonically dispersed, so that the problem of molecular agglomeration in the mixing process is avoided, the uniformity of reaction liquid is ensured, and a foundation is laid for subsequently forming a flawless separation membrane. The pillararene derivative contained in the separation membrane structure provides a macrocyclic pore structure with ion recognition capability, and triaminoguanidine hydrochloride acts on sites through multiple hydrogen bonds / ionic bonds, so that the selective separation performance of the membrane is further enhanced. Due to the collaborative design of the material, the material has precise molecular recognition capability and adjustable aperture, and can realize efficient selective separation of target objects such as small organic molecules, ions or dyes.

The present disclosure relates to methods and kits for analyzing a macromolecule. In some embodiments, the present disclosure relates to macromolecule analysis methods which employ barcoding and nucleic acid encoding of molecular recognition events. Also provided herein is a method and related kits for transferring information using a plurality of enzymes, including for performing a ligation, extension, and cleavage reaction with nucleic acid molecules associated with the macromolecule for analysis. In some embodiments, the macromolecule for analysis comprises a peptide, a polypeptide, or a protein.

The application belongs to the technical field of organic chemical synthesis, and particularly relates to a nitrogen center chiral base derivative, a preparation method and application thereof. The preparation method of the nitrogen center chiral base derivative provided by the application can effectively synthesize a base with only a nitrogen chiral center by using a tetrahydrodibenzo-diazocine compound as a raw material and through chiral phosphoric acid catalyzed amination reaction. Moreover, the synthesis method provided by the application has the advantages of high efficiency, simple steps and strong enantioselectivity, is suitable for industrialized production of chiral bases and derivatives thereof, and provides a new approach for research and application of chiral compounds. Meanwhile, the base derivative prepared by the application is a base molecule with a nitrogen center chirality, and has wide application prospects in the fields of molecular recognition, DNA binding, supramolecular chemistry, material science, asymmetric catalysis and drug development.

The invention belongs to the field of coordination polymers, and discloses an antiferromagnetic tetranuclear cobalt coordination polymer constructed by mixed polydentate ligands and a preparation method and application thereof. The chemical formula of the polymer is (C9H9O6) 2 (C26H18N4) 2 (OH) 2Co4. (H2O) 7, C9H9O6 is 1, 3, 5-cyclohexane tricarboxylic acid, C26H18N4 is 1, 2, 4, 5-tetrapyridyl benzene, the polymer is a monoclinic system and a P21 / c space group, the cell parameters are alpha = 90 degrees, beta = 96.166 (8) degrees and gamma = 90 degrees, and the polymer has few defects, high crystallinity and good stability and can be applied to the fields of adsorption separation, hydrogen storage, energy storage, molecular magnets, catalysis, sensing or molecular recognition and the like.

The present invention discloses a dual-mode intelligent substance detection method based on bivalent nucleic acid aptamers, which includes two detection modes: fluorescence and colorimetry. This method belongs to the field of analytical chemistry. The present invention combines a bivalent aptamer with a double hairpin structure with its complementary sequence. Under the action of sodium borohydride and silver nitrate, DNA-silver nanoclusters and DNA-adsorbed silver nanoparticles are formed at different times and temperatures, respectively. In both detection modes, the bivalent aptamer acts as a molecular recognition target, and its complementary sequence acts as a signal-enhancing probe. Rapid, ultrasensitive detection is achieved by detecting fluorescence, absorbance, and analyzing the R+B value of colorimetric images using a smartphone app. This detection method is simple to operate, efficient, and sensitive, and exhibits good recovery and anti-interference capabilities in spiked sample testing. It has promising application prospects in the qualitative and quantitative analysis of aminoglycoside antibiotic compounds.

The invention discloses an application of fat-soluble ring-opened cucurbituril in specific recognition of organic compounds in organic solvents, the fat-soluble ring-opened cucurbituril is composed of glycoluril tetramer and terminal aromatic ring, the terminal aromatic ring is modified by phosphate side chain, and the terminal aromatic ring has good solubility in various organic solvents; according to the invention, the C-shaped adjustable cavity of the fat-soluble ring-opened cucurbituril is matched with the size of a guest molecule, and is matched with hydrogen bonds, pi-pi interaction and the like, so that efficient molecular recognition of organic matters in an organic solvent is realized; the application of the supramolecular macrocyclic main body in organic solvents is expanded by utilizing fat-soluble ring-opening cucurbituril, and the supramolecular macrocyclic main body is expected to be applied to the fields of medicine, chemical industry, environment and the like.

The invention discloses a sensing detection application and a sensor for identifying 6PPD and 6PPD-Q based on a cyclodextrin subject and object, and relates to the technical field of pollutant detection. Specifically, the invention provides an application of at least one of cyclodextrin, functional group modified cyclodextrin and polycyclodextrin in detection of 6PPD or 6PPD-Q. According to the invention, cyclodextrin, functional group modified cyclodextrin and polycyclodextrin can selectively recognize and combine with 6PPD and 6PPD-Q hydrophobic molecules through a subject-object inclusion effect. Cyclodextrin, functional group modified cyclodextrin and polycyclodextrin can be used as ideal molecular recognition elements of 6PPD and 6PPD-Q, have high selectivity and specificity, are convenient and fast to operate, can realize linear response to 6PPD in a concentration range of 0.01-30 [mu] M, and have a detection limit as low as 4.13 nM.

The present disclosure describes methods of detecting viral biomolecules such as viruses through frequency response. A method (200) of detecting a vims includes exposing (210) a sensor surface to a fluid sample containing a suspected virus. The sensor surface can be a surface of a resonator having a clean resonant frequency from about 1 MHz to about 1 GHz. The surface can be modified with molecular recognition groups selective for binding to the viral biomolecule. A resonant frequency of the resonator can be measured (220) after exposing the sensor surface to the fluid sample. The measured resonant frequency can be compared (230) with a clean resonant frequency indicating the presence of the viral biomolecule bound to the molecular recognition groups and then outputted (240) as a detection signal.

The invention discloses a water-soluble fluorescent conjugated polyelectrolyte as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out Suzuki coupling reaction or Sonogashira coupling reaction polymerization on the synthesized 3, 6-diborate carbazole or 3, 6-diethynyl carbazole monomer, and then carrying out quaternization reaction to obtain the conjugated polyelectrolyte. The 3, 6-substituted carbazole is introduced into a polymer main chain to form a non-rigid structure, so that the solubility of the polymer in a solvent is improved, and the blue fluorescence quantum yield is increased; meanwhile, the trimethylamine quaternary ammonium salt side group not only enhances the water solubility, but also is used as a molecular recognition site to be combined with negative charges of heparin through electrostatic interaction; a fluorescent sensing system is formed after the conjugated polyelectrolyte is dissolved in a buffer solution, and the fluorescence intensity of the fluorescent sensing system is remarkably quenched and responded along with increase of the heparin concentration. The heparin quantification range can be flexibly adjusted by adjusting the concentration of the conjugated polyelectrolyte; the method is suitable for detection of common heparin and low-molecular-weight heparin and analysis of heparin in complex samples.

This invention discloses a method for synthesizing potassium 4-methoxysalicylate based on supramolecular-assisted methylation, belonging to the field of organic synthesis technology. The process is based on supramolecular chemistry principles and includes the following steps: (1) Preparation of a macrocyclic host molecule-methylating agent complex loaded with a methylating agent: The macrocyclic host molecule and the methylating agent are reacted at pH 7-9 and 25-40℃ to form a complex solution; (2) One-pot preparation of the target product: 2,4-dihydroxybenzoic acid is salted with potassium hydroxide, potassium carbonate is added, and the above complex solution is added dropwise. A selective methylation reaction is carried out at 55-65℃. After the reaction is complete, post-treatment yields the product. This invention utilizes the molecular recognition function of the macrocyclic host to achieve highly selective methylation of the 4-hydroxyl group under mild conditions. This process has the advantages of simple operation, high safety, low waste, and recyclable host molecules. The yield of potassium 4-methoxysalicylate can reach over 85%, and the purity exceeds 99.0%.

The invention relates to the technical field of biological medicines, in particular to a bupivacaine sustained-release injection as well as a preparation method and application thereof. The invention provides a bupivacaine drug compound. The bupivacaine drug compound contains an amide local anesthetic bupivacaine as an active component and a plurality of pharmaceutically acceptable slow-release carrier materials. The drug compound disclosed by the invention adopts a supramolecular synthesis strategy and is prepared into nano-particles through a self-assembly method of a molecular recognition mechanism between Ad and Cd. The novel bupivacaine nano-particles provided by the invention solve the problem of too fast release of the bupivacaine hydrochloride, the preparation method is simple, flexible and modularized, the nano-preparation with controllable carrier size, stable surface chemical property and high drug loading capacity is prepared, the slow release time in vivo can be prolonged, and the analgesic effect can be continuously exerted.

The invention discloses an immunochromatography detection kit for pesticide residues in food and a detection method, and relates to the technical field of food detection.The kit comprises an immunochromatography test strip and a sample extracting solution, the immunochromatography test strip is formed by sequentially overlapping and pasting a PVC bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, the lap joint width of the adjacent parts is 2-3mm; the synergistic effect of all parts of the immunochromatography test strip is optimized, a detection system with high targeting property is constructed, target pesticide residues are accurately captured and responded by virtue of specific recognition of a target triggering type hydrogel switch, the matrix influence is reduced by matching with a special sample extracting solution, and meanwhile, the molecular recognition and immunochromatography technologies are integrated, so that the detection efficiency is greatly improved. The target trigger type hydrogel switch has the advantages of high selectivity, stable signal marker activity, reasonable layout of a detection line and a quality control line, simplified pretreatment, simple operation, meeting of multi-scene batch detection, and provision of support for food quality safety supervision.

This application discloses a literature information extraction system for the field of chemistry, including a document parsing and preprocessing module, a general file cleaning module, an image processing module, an extraction and post-processing module, and a file integration and output module. The document parsing and preprocessing module is configured to parse and convert acquired PDF files in the field of chemistry, generating an initial general file, an image set, and literature citation information. The initial general file includes image links. The general file cleaning module is configured to perform text preprocessing on the initial general file, generating target general text. The image processing module is configured to sequentially perform molecular recognition processing on each image in the image set, generating image text description information. The extraction and post-processing module is configured to extract the target general text and image text description information, generating multiple types of extraction results. The file integration and output module is configured to integrate the multiple types of extraction results, inject literature citation information, and output the target file.

The invention belongs to the technical field of chemical separation, and discloses a separation method of a high-boiling-point component in high-carbon aldehyde production. The method is characterized in that 0.05%-2.0% of functional ionic liquid is added into a rectification system, and the apparent vapor pressure of impurities is directionally inhibited and the relative volatility is remarkably improved by utilizing the selective hydrogen-bond interaction between anions of the functional ionic liquid and hydroxyl / carbonyl in the impurities. According to the method, the ionic liquid with a molecular recognition function is introduced, the phase equilibrium relation between the high-carbon aldehyde and the high-boiling-point impurities is reconstructed from the thermodynamic essence level, the inherent limitation that traditional rectification depends on weak physical property differences is broken through, efficient, low-energy-consumption and high-stability purification of the high-carbon aldehyde product is achieved, the ionic liquid can be recycled, and the method is suitable for industrial production. The invention provides a novel high-carbon aldehyde purification method which has the advantages of novel principle, simplicity and convenience in operation, high efficiency, energy conservation and industrial feasibility.

The invention discloses a preparation method of a two-dimensional chiral MXene membrane based on carboxymethyl-beta-cyclodextrin modification and application of the two-dimensional chiral MXene membrane in the aspect of enantiomer separation. The core of the invention lies in that the cyclodextrin is stably and orderly fixed in a two-dimensional mass transfer channel through a covalent reaction between carboxymethyl of the cyclodextrin and a functional group on the surface of MXene by utilizing the molecular recognition capability of a cyclodextrin cavity; furthermore, the highly organized cyclodextrin units are used as accurate'chiral probes' and cooperate with the high permeability of an MXene channel, so that efficient screening of enantiomers is realized, and meanwhile, excellent separation selectivity and flux are guaranteed.

The application belongs to the technical field of molecular detection, and discloses a medicinal material identification system and method; the method comprises the following steps: emitting a topological protection acoustic wave to concentrate DNA of medicinal materials, marking the DNA with a CdTe quantum dot-nucleic acid probe, scraping the sample to extract DNA and performing PCR amplification, designing a logic gate staple chain to prepare a light-controlled origami probe, and injecting a PZT chip nano-pore array to be light-activated, and then, through AFM analysis of free energy, the medicinal materials are identified; the application constructs a complete technical chain from molecular recognition to reliable storage, and comprehensively solves multiple technical bottlenecks of traditional methods in medicinal material identification.

The invention discloses a multi-channel anti-interference based efficient detection method for natural active components of plants, which comprises the following steps: a multi-channel micro-fluidic chip is adopted to detect the natural active components of the plants, and the multi-channel micro-fluidic chip comprises a plurality of independent channels; a molecularly imprinted polymer with specific molecular recognition capability is preloaded in the independent channel, and natural active ingredients of the plant are captured by the molecularly imprinted polymer under the assistance of a pulsed electric field; the elution buffer solution flushes the independent channels, and the natural active ingredients of the plants enter a detection area; the natural active ingredients of the plants are detected by adopting ultraviolet and fluorescent dual modes; carrying out real-time denoising on a detection result by adopting an AI algorithm; and matching the de-noised detection result with a standard spectrum library, and outputting a component content and purity report. By adopting multi-channel synergistic separation and multi-channel synchronous treatment, the detection efficiency is improved, the single detection time is less than or equal to 30 minutes, the molecularly imprinted polymer filler is combined with the pulsed electric field, and the target component recovery rate is greater than or equal to 90%.

The invention relates to the technical field of alcohol detection, in particular to a preparation method of a metal-based energy-consumption-free alcohol detection material and an application method of the metal-based energy-consumption-free alcohol detection material in alcohol concentration detection and alcohol molecule recognition. A continuous groove micro-nano structure is machined on the surface of a substrate material, chemical composition is changed, and the continuous groove micro-nano structure comprises a plurality of grooves with preset widths, perpendicular to the length direction, in a laser processing area with the preset length; the depth of the groove is 8-12 microns, and the width of the groove is 30-40 microns; the alcohol concentration can be identified according to the diffusion length of alcohol on the surface, and meanwhile whether gas contains alcohol or not can be detected by changing the surface contact angle and the rolling angle. The substrate material is convenient and rapid to prepare and low in cost, and the method for detecting the alcohol concentration and identifying the alcohol molecules is simple to operate, saves a detection solvent and is short in time consumption.