Preparation method of macrolide antibiotics molecular engram polymer microsphere

A technology of macrolides and molecular imprinting, which is applied to the preparation of microspheres, microcapsule preparations, alkali metal compounds, etc., and can solve the problems of inability to prepare molecularly imprinted polymer microspheres of macrolide antibiotics

Inactive Publication Date: 2009-08-19
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the deficiency that molecularly imprinted polymer microspheres of macrolide antibiotics cannot be prepared in aqueous phase by molecular imprinting technology in the prior art, the presen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Weigh 0.622g of polyvinyl alcohol and add it to 100ml double distilled water, heat up to 80°C with stirring to dissolve it, and form an aqueous dispersion, cool to room temperature and transfer to a 250ml reactor. Weigh 0.734g of erythromycin and dissolve it in 10ml of toluene, add 0.4ml of acrylic acid, and apply ultrasound for 40min at 30°C to make the erythromycin and acrylic acid fully react to form a complex, then add 4.655g of ethylene glycol dimethyl bis Acrylate, ultrasonic 10min, into oil phase mixture. Slowly add 1 part of the oil phase mixed liquid into 6 parts of the aqueous phase dispersion under stirring at 450r / min, and the dropping speed is controlled at 2ml / min to form a milky white microsuspension emulsion. Access to N 2 15min, N 2 The flow rate is 0.5ml / min. Then 0.0760 g of azobisisobutyronitrile was added and stirred at 400 r / min, and thermally initiated polymerization in a water bath at 50° C. for 24 hours. After cooling to room tem...

Embodiment 2

[0022] Example 2: Weigh 0.0451 g of hydroxyethyl cellulose and add it to 90 ml of double-distilled water, heat up to 50° C. to dissolve it under stirring, and form an aqueous dispersion. After cooling to room temperature, transfer it to a 250 ml reactor. Weigh 0.734g of erythromycin and dissolve it in 7.5ml of acetonitrile, add 0.4ml of α-methacrylic acid, and apply ultrasound for 30min at 25°C to fully react the erythromycin and methacrylic acid to form a complex, then add 3.960g of acetonitrile Glycol dimethyl diacrylate, sonicated for 20 minutes, formed an oil phase mixture. Slowly add 1 part of the oil phase mixed liquid into 14 parts of the aqueous phase dispersion under stirring at 400r / min, and the dropping speed is controlled at 3ml / min to form a milky white microsuspension emulsion. Access to N 2 30min, N 2 The flow rate is 1ml / min. Then 0.0760 g of azobisisobutyronitrile was added and stirred, the stirring condition was 500 r / min, and the polymerization was therm...

Embodiment 3

[0025] Example 3: Weigh 0.055 g of hydroxyethyl cellulose and add it to 10 ml of double-distilled water, heat up to 80° C. to dissolve it under stirring, and form an aqueous dispersion, and transfer it to a 250 ml reactor after cooling to room temperature. Weigh 0.734g of erythromycin and dissolve it in 7.5ml of acetonitrile, add 0.4ml of α-methacrylic acid, and apply ultrasound at 20°C for 50 minutes to fully react erythromycin and α-methacrylic acid to form a complex, then add 3.960 g ethylene glycol dimethyl diacrylate, ultrasonic 10min, an oil phase mixture. Slowly add 1 part of the oil-phase mixed liquid into 7 parts of the aqueous phase dispersion under stirring at 500 r / min, and the dropping speed is controlled at 2 ml / min to form a milky white micro-suspoemulsion. Access to N 2 20min, N 2 The flow rate is 0.6ml / min. Then 0.0760 g of azobisisobutyronitrile was added and stirred at 450 r / min, and the polymerization was thermally initiated in a water bath at 65° C. fo...

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Abstract

The invention discloses a method for preparing macrolide antibiotics molecular engram polymer microspheres, which is characterized by comprising the following steps: dissolving a dispersant, namely polyvinyl alcohol or hydroxyethyl cellulose into secondary distilled water to prepare a water-phase dispersion liquid; dissolving engram molecules and functional monomers into an organic solvent to prepare an oil-phase mixture; adding the oil-phase mixture into the water-phase dispersion liquid under the action of stirring, adding an initiator, namely azo-bis-iso-butyrynitrile into the mixture, performing thermal initiation polymerization on the mixture in water bath, and obtaining polymer microspheres; and adopting an ultrasonic extraction method to elute the engram molecules in a butyl acetate aqueous solution or a methanol solution of acetic acid, using distilled water to wash the engram molecules, and performing vacuum drying on the engram molecules to obtain the macrolide antibiotics molecular engram polymer microspheres. Through the method, the macrolide antibiotics molecular engram polymer microspheres are prepared in water phases and are recognized in the water phases; the reorganization result is close to that obtained by a natural biological molecular recognition system; and the invention provides a method for recognizing, separating and analyzing hydrophilic medicaments .

Description

technical field [0001] The invention relates to a method for preparing molecularly imprinted polymer microspheres, in particular to a method for preparing macrolide antibiotic molecularly imprinted polymer microspheres. Background technique [0002] Macrolide antibiotics are basic medium-spectrum antibiotics that are widely used. In terms of chemical structure, they are all based on 12-16-membered macrolides, linked by glycoside bonds and 1-3 molecules of sugar. polyoxymacrolide antibiotics. Erythromycin (EM) and roxithromycin are the most commonly used 14-membered macrolide antibiotics. Azithromycin is a semi-synthetic C-9 tertiary amine derivative of a 15-membered lactone ring, and midecamycin and acetylspiramycin are semi-synthetic 16-membered macrolide antibiotics. Semi-synthetic midecamycin and spiramycin derivatives. Erythromycin is a biological fermentation antibiotic, and the rest are semi-synthetic macrolide antibiotics. [0003] In the fermentation system and s...

Claims

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Application Information

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IPC IPC(8): B01J20/285B01J20/30B01J20/28B01J13/14
Inventor 胡小玲管萍朱丽张新丽
Owner NORTHWESTERN POLYTECHNICAL UNIV
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