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Method for preparing nano-cellulose antibacterial composite material through on-line culture

A technology of nanocellulose and composite materials, which is applied in the field of on-line culture and preparation of nanocellulose antibacterial composite materials, can solve the problems of poor drug loading and sustained release, inability to absorb in time, secondary trauma, etc., to save fermentation time, The effect of shortening the production cycle and increasing production efficiency

Active Publication Date: 2012-07-11
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] On the other hand, gauze is a traditional textile-based dressing for traumatology. At present, this type of dressing has the following problems: (1) its water absorption performance is poor, and its moisturizing ability is insufficient; (2) its ability to load and release drugs is poor; (3) it is easy to Adhesion to the wound, causing secondary trauma when changing the dressing; (4) Can not absorb body fluids and other substances secreted by the wound tissue in time; (5) The physical barrier effect of single-layer gauze is poor, unable to block dust and microorganisms in the air, Can not provide a relatively moist and sterile environment for wound healing, can not promote wound healing
The above defects limit the application of textile-based dressings in high-end products
Traditional gauze dressings are increasingly unable to meet people's requirements for wound management

Method used

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  • Method for preparing nano-cellulose antibacterial composite material through on-line culture
  • Method for preparing nano-cellulose antibacterial composite material through on-line culture
  • Method for preparing nano-cellulose antibacterial composite material through on-line culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Bacteria culture

[0042] Insert Acetobacter xylinum into 300mL liquid medium (per 1L of water, 20g of mannitol, 3g of peptone, 5g of yeast extract, pH 3.0, sterilized at 121°C for 20min; or 200g of glucose, 5g of yeast extract, Tryptone 5g, citric acid 1.15g, Na 2 HPO 4 2.7g, 1L of water, pH 7.5, sterilized at 121°C for 20min) for expansion, cultured on a shaking table at 20°C, 100r / min or standing for 12h before use;

[0043] (2) Fermentation preparation of bacterial cellulose composite antibacterial film

[0044] The liquid culture medium containing the production strain prepared in step (1) is transferred to a bioreactor equipped with a fixed gauze or silk skeleton material drum, and then the drum rotates at a speed of 7, 15, 30 and 60rpm for disturbance cultivation After 1 day of dynamic culture at 30°C, add different concentrations of chitosan solutions to the culture medium regularly, and then culture for 2-7 days to obtain bacterial cellulose / gauze / chitos...

Embodiment 2

[0055] (1) Bacteria culture

[0056] Put kombucha (kombucha) into 300mL liquid seed culture medium (per 1L of water, green tea 5g, glucose 10, peptone 3g, yeast extract 5g, pH3 .0, pasteurized for 30min; per 1L of water, 100g of glucose, 3g of peptone, 5g of yeast extract, pH 7.5, sterilized at 121°C for 20min) to expand culture, cultured on a shaking table at 25°C, 150r / min or After 24 hours of static culture, it is ready for use;

[0057] (2) Fermentation preparation of bacterial cellulose antibacterial composite film

[0058] Transfer the liquid culture medium containing the production strain prepared in step (1) to a bioreactor equipped with a drum fixed with a polyester, nylon or polypropylene skeleton material, then rotate the drum to carry out disturbance cultivation at a speed of 15 rpm, at 30 ° C After 1 day of dynamic culture, regularly add different concentrations of chitosan solutions to the culture medium, and then harvest the film-forming product after 2-7 days...

Embodiment 3

[0064] (1) Bacteria culture

[0065] Insert Gluconacetobacter xylinus into 300mL liquid medium (per 1L of water, maltose 100g, peptone 3g, yeast extract 5g, pH 4.5, sterilized at 121°C for 20min; or fructose 160g, yeast extract 5g, tryptone 5g, citric acid 1.15g, Na 2 HPO 4 2.7g, 1L of water, pH 6.0, sterilized at 121°C for 20min) for expansion, cultured on a shaking table at 30°C, 250r / min or standing for 48h before use;

[0066] (2) Fermentation preparation of bacterial cellulose antibacterial composite film

[0067] Transfer the liquid culture medium containing the production strain prepared in step (1) to a bioreactor equipped with a titanium metal mesh drum, then rotate the drum at a speed of 15 rpm for disturbed culture, and after dynamic culture at 30°C for 1 day, periodically Add different concentrations of sodium alginate solutions to the culture medium, and then cultivate for 7 days to obtain the bacterial cellulose antibacterial composite film;

[0068] Or get t...

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Abstract

The invention relates to a method for preparing a nano-cellulose antibacterial composite material through on-line culture. The method comprises the following steps of: (1) inoculating an activated bacterial cellulose producing strain into a liquid culture medium, performing amplification culture, transferring the liquid culture medium into a bioreactor, culturing, adding an antibacterial material into the liquid culture medium, and continuing to culture to obtain an unpurified antibacterial bacterial cellulose composite material; and (2) peeling a bacterial cellulose membrane from a framework or directly soaking the composite material in a NaOH solution, treating in water bath, and washing until an obtained product is neutral to obtain the nano-cellulose antibacterial composite material. The preparation efficiency is high, and the method is simple, convenient, feasible and low in cost; and the surface of the bacterial cellulose composite material has a nanoscale three-dimensional fibrous mesh-shaped structure, the tensile strength of the material is greatly improved compared with that of a pure bacterial cellulose membrane, and the material can be widely applied to products such as facial masks, wound dressings, plasters, artificial skins and the like.

Description

technical field [0001] The invention belongs to the field of preparation of bacterial cellulose composite materials, in particular to a method for preparing nano-cellulose antibacterial composite materials by on-line culture. Background technique [0002] The unique three-dimensional nanofiber network structure of bacterial cellulose (BC) membrane endows it with good air permeability and water permeability, and can absorb 60 to 700 times its dry weight of water, all of which are in the form of free water exist. Compared with other artificial skin and trauma dressings, the main feature of this film is that it has good compatibility with the skin and is non-irritating; the nanocellulose fibers will not stick to the wound, will not cause secondary damage, and will not There is residue; it has good permeability to liquid, gas and electrolyte in wet conditions, can quickly absorb wound blood and tissue fluid, prevent wound infection and suppuration, and can provide a moist envir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/44A61L27/60A61K8/02A61K8/73C12P19/04C12N1/20A61Q19/00C12R1/02C12R1/01C12R1/41C12R1/38C12R1/025C12R1/05C12R1/065
Inventor 洪枫
Owner DONGHUA UNIV
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