20 results about "Tryptone" patented technology

The application discloses a fabric yellowing reagent and a fabric yellowing method, and relates to the technical field of daily chemical products. 5 The fabric yellowing reagent comprises 1-3% of histidine solution and a bacterial suspension with a bacterial concentration of 1*10 5 CFU / mL-9*10 The bacteria are a mixture of one or more of Staphylococcus epidermidis and Micrococcus luteus; the solvent of the histidine solution is normal saline containing 0.15-1% of trypsin peptone; and the solvent of the bacterial suspension is normal saline containing 0.1-0.2% of trypsin soybean peptone liquid culture medium. The fabric yellowing reagent can be used for simulating fabric yellowing, and has good operability, reproducibility and data reliability.

The invention belongs to the technical field of microorganisms, and discloses a high-density fermentation process of vagina-sourced lactobacillus crispatus. The invention discloses a fermentation medium which comprises glucose, tryptone, beef extract, yeast extract, dipotassium phosphate, ammonium citrate, L-cysteine or salt thereof, magnesium sulfate and manganese sulfate. According to the fermentation culture medium provided by the invention, through scientific compounding of a carbon source, a nitrogen source and key factors, the final viable concentration and thallus activity of vagina-derived lactobacillus crispatus can be remarkably improved. The fermentation culture medium is adaptive to the micro-aerobic metabolism characteristics of vagina-derived lactobacillus crispatus by precisely regulating and controlling the dissolved oxygen level (initial dissolved oxygen is controlled to be 5% or below), and when the fermentation culture medium is used for culturing lactobacillus crispatus, the fermentation delay period can be remarkably shortened to 2-3 h, the final viable bacterium yield is greatly increased to 1 * 10 < 10 > CFU / mL or above, and the production cost is reduced. And an efficient and stable technical support is provided for industrial production of the probiotic preparation.

The application discloses a composition capable of effectively inhibiting the growth of Staphylococcus aureus biofilm. The application firstly optimizes the nitrogen source in the culture medium of Staphylococcus epidermidis CCSM0287 strain, fully explores the growth difference of Staphylococcus epidermidis under different culture media and culture conditions and the inhibition effect of the fermentation supernatant on Staphylococcus aureus biofilm, then compounds the fermentation supernatant of Staphylococcus epidermidis CCSM0287 obtained by using the optimized culture medium (the nitrogen source adopts tryptone + soybean peptone + fresh beef bone collagen peptide powder) with the fermentation supernatant of Lactobacillus paracasei S20, can obviously inhibit the growth of Staphylococcus aureus biofilm and destroy the surface structure of the Staphylococcus aureus biofilm, and can be applied in bacteriostatic products.

The invention relates to a preparation method of a bacterial cellulose composite nano-silver biological dressing, and belongs to the technical field of biological material preparation. Enzymatic hydrolysate of bean pulp, alfalfa leaching liquor and tryptone are used as fermentation culture media, acetobacter xylinum seed liquid is added, the bacterial cellulose membrane is obtained, and the content of the bacterial cellulose membrane composite nano-silver biological dressing is limited to 50-99% of water. Comprising chitosan, gamma-polyglutamic acid, beta-glucan, refined water and silver ions. The bacterial cellulose membrane composite nano-silver biological dressing has the effects that the bacterial cellulose membrane composite nano-silver biological dressing is high in mechanical strength, high in water-retaining property, good in biocompatibility, good in exudate absorption capacity and non-toxic, and as the nano-silver biological dressing for wounds, the cellulose composite nano-silver biological dressing can provide a moist environment, is beneficial to rapid cleaning of the wounds, and has a good application prospect. Meanwhile, the antibacterial activity of chitosan, gamma-polyglutamic acid, beta-glucan, silver ions and the like on bacteria is more beneficial to wound healing.

The invention relates to the technical field of microorganisms, and discloses an improved TSA bouillon culture medium and application thereof.The improved TSA bouillon culture medium comprises tryptone, sodium chloride, soybean peptone, agar, Rpf recombinant protein and magnesium sulfate, and the mass concentration ratio of tryptone to sodium chloride to soybean peptone to agar to Rpf recombinant protein to magnesium sulfate is (10 g / L-20 g / L) to (4 g / L-6 g / L) to (3 g / L-7 g / L) to (13 g / L-17 g / L) to (10 mu g / L-100 mu g / L) to (0.5 g / L-0.7 g / L). The improved TSA broth culture medium obtained by the invention can be used for successfully culturing Escherichia coli in a VBNC state and staphylococcus aureus in the VBNC state.

The invention relates to the technical field of microbial fermentation, and particularly provides a high-density fermentation method for improving immunogenicity of duck escherichia coli. The high-density basic culture medium is prepared from the following components: 2 to 15g / L of yeast extract, 5 to 20g / L of tryptone, 34 to 36g / L of KH2PO4, 1.6 to 2.0 g / L of (NH4) 2HPO4, 1.5 to 2.5 g / L of NaCl, 0.5 to 3g / L of citric acid, 0.5 to 2g / L of MgSO4, 2 to 20g / L of glucose and 0.01 to 0.1 g / L of VB1. And the high-density fed culture medium comprises 50-500g / L of glucose, 10-50g / L of yeast extract and 20-100g / L of tryptone. Compared with the prior art, large-scale high-density fermentation of duck Escherichia coli can be realized by using the two, and the immunogenicity of duck Escherichia coli can be improved.

The invention discloses a culture method for promoting the growth of floating moss of amphibious moss plants by using nitrogen-fixing bacteria, which remarkably improves the growth speed and biomass of the floating moss of the amphibious moss plants by co-culturing the floating moss and the nitrogen-fixing bacteria so as to achieve a better growth promotion effect. The nitrogen-fixing bacteria disclosed by the invention are Rhodopseudomonas palustris, and the co-culture method comprises the following steps of: activating the Rhodopseudomonas palustris, and culturing the activated Rhodopseudomonas palustris in a tryptone soybean liquid culture medium. When the bacterial liquid is turbid and the O.D600 value is measured to be about 1, sucking the bacterial liquid on a culture medium containing 1 / 2GB5 and 1% of cane sugar, coating by using a coating device, and inoculating floating moss on a flat plate containing the bacterial liquid; the method provided by the invention can obviously promote the growth of floating moss biomass, and has a wide prospect in the direction of promoting plant growth.

The invention relates to the technical field of microorganisms, and discloses an improved LST broth culture medium and application, and the improved LST broth culture medium comprises tryptone, ammonium chloride, lactose, dipotassium phosphate, monopotassium phosphate, sodium lauryl sulfate, Rpf recombinant protein, magnesium sulfate, sodium pyruvate and pyridoxal phosphate. The improved LST broth culture medium disclosed by the invention can be used for successfully enriching the VBNC-state escherichia coli, so that researchers can successfully detect the VBNC-state escherichia coli in a culture manner.

The invention provides an Escherichia coli culture medium capable of efficiently promoting growth and a preparation method thereof, and belongs to the technical field of microbial culture mediums. The Escherichia coli culture medium per liter comprises the following components: 10 to 15 g of tryptone, 5 to 10 g of yeast extract, 2.5 to 3 g of glucose, 3 to 5 mL of glycerin, 4 to 6 g of sodium chloride, 5 to 10 g of dipotassium phosphate trihydrate, 1 to 5 g of potassium dihydrogen phosphate, 0.1 to 1 g of magnesium sulfate heptahydrate, 0.01 to 0.1 g of calcium chloride, 0.1 to 1 g of L-glutamine, 0.01 to 0.02 g of vitamin B group mixture, 0.5 to 2 g of betaine and an HEPES buffer solution, and the final concentration of HEPES is 10 mM. Compared with an LB culture medium, the escherichia coli culture medium has the advantages that the growth rate is increased by 55.6%, the recombinant protein expression quantity is increased by 59.1%, and the plasmid extraction yield is increased by 69.4%.

The present application relates to the technical field of fermentation medium, more specifically, it relates to a high-density fermentation medium for streptococcus suis and preparation and application thereof.The medium comprises beef infusion powder 1-5 parts, tryptone 1-10 parts, pancreatic casein peptone 1-10 parts, soybean peptone 1-5 parts, dipotassium hydrogen phosphate 1-5 parts, sodium chloride 1-10 parts, yeast extract 1-10 parts and glucose 1-5 parts.

The application relates to the field of cosmetics, and relates to beta-(1,3)-(1,6)-glucan and a preparation method and cosmetics thereof. Fermentation seed liquid is inoculated into a culture medium for fermentation; the culture medium comprises, in percentage by mass, 1-5% of glucose, 0.5-5% of tryptone, 0.1-1% of yeast extract powder, 0.01-0.1% of magnesium sulfate, 0.05-0.5% of potassium dihydrogen phosphate and the balance of water; in the culture medium, the concentration of vitamin B1 is 5-20 mg / L; and the concentration of folic acid is 1-8 mg / L. The fermentation strategy of using the culture medium containing vitamin B1 and folic acid in the above content range for fermentation can be used to prepare beta-(1,3)-(1,6)-glucan produced by schizophyllum commune, can significantly increase the weight of schizophyllum commune mycelium in the same time, effectively improves the content of beta-(1,3)-(1,6)-glucan, and has the advantages of shortening the production cycle and reducing the production cost.

The application provides a preparation method of a culture medium for improving the hydroxylation rate of recombinant collagen and application thereof, and the culture medium comprises the following components in the following concentrations: 10-15 g / L of tryptone, 5-8 g / L of yeast powder, 10-13 g / L of sodium chloride, 20-30 g / L of hydroxyproline, 0.9-2 g / L of sorbitol, 1-2 g / L of ammonium sulfate, 1-2 g / L of magnesium chloride, and 1-2.5 g / L of calcium chloride. The culture medium has no toxic side effect on the growth of bacteria, can significantly promote the growth of bacteria, and the obtained recombinant collagen has a hydroxylation rate of more than 90%, and the hydroxyproline accounts for about 20% of the amino acid sequence of the protein, which significantly exceeds the hydroxylation level of natural collagen. The culture medium has the characteristics of simple components, simple preparation, and strong universality, and provides a feasible technical scheme for large-scale preparation of high-quality recombinant collagen.

This invention discloses a selective solid blood culture medium for isolating Cryptococcus pyogenes and its application. The culture medium is a solid medium containing 15.0 g / L tryptone, 10.0 g / L yeast extract, 0.5 g / L L-cysteine, 5.0 g / L sodium chloride, and 15.0 g / L agar. After sterilization and cooling to 50°C, 5% (v / v) defibrinated sheep blood, 0.010 g / L vitamin K, 0.010 g / L heme chloride, and 0.010 g / L colistin B are added. This invention's culture medium selectively inhibits Gram-negative bacteria such as Escherichia coli by adding colistin B. Simultaneously, by optimizing the nitrogen source, adding key growth factors such as L-cysteine, vitamin K, and heme chloride, and using a simple anaerobic gas-generating bag culture method, it can significantly improve the isolation efficiency of Cryptococcus pyogenes from clinical samples, forming typical colonies within 24-48 hours, facilitating rapid pathogen isolation.

This invention discloses a Pseudomonas aeruginosa reducing agent and its application and method in vanadium reduction. Pseudomonas bariensis is inoculated into a vanadium-containing solution to reduce pentavalent vanadium ions. The concentration of pentavalent vanadium in the vanadium-containing solution is 0.5–10 mM. The vanadium-containing solution also includes high-salt LB medium, which is composed of: 10.0 g / L tryptone, 5.0 g / L yeast extract, and 30.0 g / L NaCl, prepared with sterile water. The pH is adjusted to 7.0 with 1 mol / L NaOH or 1 mol / L HCl, and the medium is autoclaved at 121°C for 20 min before use. The reduction reaction of the Pseudomonas aeruginosa and the vanadium-containing solution is carried out at 28°C, 150 rpm oscillation frequency, under anaerobic and dark conditions. The *Pseudomonas baralicum* provided by this invention can still grow when exposed to concentrations up to 10 mM V(V). When the vanadium concentration is 0.5, 1.0, 2.0, 5.0, and 10 mM, the reduction rate of V(V) by this *Pseudomonas baralicum* is 81.1%, 72.4%, 72.3%, 70.8%, and 60.6%, respectively, indicating that *Pseudomonas baralicum* can be used as a reducing agent to remove vanadium toxicity from vanadium-contaminated environments.

The application discloses a fermentation process of gonadotropin-releasing hormone hexamer-diphtheria toxin mutant CRM197, and the process comprises the following steps: screening of a fermentation medium and optimization of fermentation conditions. The fermentation medium comprises yeast extract powder, tryptone and NaCl, and the optimization of the fermentation process comprises culture temperature, an inducing agent, concentration of the inducing agent, culture time after induction, adjustment mode of pH of the fermentation liquor, stirring speed, ventilation amount and the like. The fermentation process has the advantages of low production cost, short production cycle (only 12-15 hours are needed), improved expression amount of the target protein per unit of bacteria, and realized high-efficiency expression of the target protein. The GnRH6-CRM197 prepared by the application can be used for immunocastration of various mammals, and has the values of further research and development and good industrialization prospect.

The invention discloses a toxin-producing culture medium of C-type clostridium botulinum toxin as well as a preparation method and application of the toxin-producing culture medium. Belongs to the technical field of veterinary vaccine preparation. The toxin-producing culture medium provided by the invention is prepared from the following components in parts by mass: 5 to 10g / L of glucose, 35 to 45g / L of tryptone, 10 to 15g / L of yeast extract powder, 5 to 7.5 g / L of KH2PO4, 0.2 to 0.3 g / L of MgSO4. 7H2O, 0.5 to 0.75 g / L of cysteine hydrochloride and 0.01 to 0.015 g / L of hemin. The toxin-producing culture medium does not contain animal-derived components, can ensure that the C-type clostridium botulinum toxin liquid with stable quality and high toxin content can be obtained, and can be used for producing C-type clostridium botulinum toxin vaccines with stable quality.

The invention discloses a method for enriching staphylococcus aureus in food. Putting the food into the improved casein tryptone soybean broth for enrichment culture; the improved casein tryptone soybean broth is divided into A and B; wherein A is 4% sodium chloride casein tryptone soybean broth, and B is a slow-release material wrapping sodium chloride; in the enrichment culture process, the component B slowly releases sodium chloride in the component A, so that the sodium chloride content in the enrichment culture medium is finally about 10%. According to the method, repair and recovery of the damaged staphylococcus aureus in the food are promoted, and growth and interference of other non-target bacteria are inhibited through high salt, so that effective proliferation and growth of the damaged staphylococcus aureus in the food are promoted, the detection rate is increased, potential safety hazards of the food are eliminated, and health of consumers is prevented from being harmed.

The invention belongs to the field of microbial fermentation, and relates to a method and a composition for increasing the yield of propionic acid based on synergy fermentation of congener and heterogeneity double bacteria, and the method comprises the following steps: providing a first propionibacterium freudenreichii subsp. Scheri PB-16 and a second propionibacterium capable of producing propionibacterium BY-62, which are respectively propionibacterium freudenreichii subsp. Scheri PB-16; inoculating the first propionibacterium and the second propionibacterium into a fermentation culture medium according to a volume ratio of 0.8: 1 to 1.2: 1; standing and culturing for 5-7 days at 30 DEG C, and shaking and uniformly mixing twice in the morning and evening every day during the period; the fermentation medium comprises 8-12 g / L of yeast extract, 2-3 g / L of tryptone, 18-22 g / L of glycerin, 0.8-1.2 g / L of dipotassium phosphate, 2-3 g / L of glucose and 8-12 g / L of calcium carbonate, and the pH value is adjusted to 7.0-8.0. The yield of propionic acid can be increased, the heteroacid level and the purification cost are reduced, and the process is simple, feasible, easy to operate and suitable for industrial large-scale production.

The invention relates to the technical field of microbiological detection, and particularly discloses a culture medium for selectively separating and culturing Burkholderia cepacia, which comprises the following components: 8-12 g / L of tryptone, 8-12 g / L of sucrose, 1-2 g / L of yeast extract, 1-2 g / L of monopotassium phosphate, 5-10 g / L of sodium pyruvate, 4-7 g / L of sodium chloride, 15-23 mg / L of phenol red, 10-18 g / L of agar, 0.8-1.3 mg / L of crystal violet, 0.5-5 g / L of selective inhibitor, and the balance of water. 10-30 g / L of a specific accelerant and the balance of water; the selective inhibitor is selected from one or more of sodium deoxycholate, sodium taurocholate, sodium glycocholate and propyl gallate; the specific accelerant is lactose, cane sugar and vanillic acid; and the culture medium does not contain any antibiotic component. The culture medium disclosed by the invention is free of antibiotic components, good in selectivity and high in growth promoting ability, and has a very good market application prospect.

The invention belongs to the technical field of microbiological detection, and particularly provides a clostridium perfringens semi-quantitative detection kit, a detection method and application, the clostridium perfringens semi-quantitative detection kit comprises a liquid reagent A and a solid reagent B; the liquid reagent A comprises an aqueous solution in which tryptone, soy peptone, yeast extract powder and ammonium ferric citrate are dissolved; the solid reagent B comprises sodium pyrosulfite. According to the clostridium perfringens semi-quantitative detection kit provided by the invention, the specific liquid culture medium with an optimized formula is adopted, and the reagent A and the reagent B adopt a liquid-solid split charging design, so that sodium pyrosulfite is prevented from being degraded in a liquid environment, and the shelf life is prolonged. And a standard colorimetric card and a time comparison table can be prepared, so that subjective errors are reduced. In addition, the device can be integrated with a disposable sampling tool, a portable constant-temperature water bath cup and a timing prompt assembly to form a complete detection system which can be used after being opened, and the single detection cost is low.