310 results about "Tryptone" patented technology

The invention discloses a method for breeding test-tube plantlets of Paphiopedilum henryanum Braem seeds, comprising seed bourgeoning, proliferation and differentiation, and sound seedling rootage. A culture medium for the seed bourgeoning includes 1/4 of MS, 15 percent (in volume) of coconut milk, 20 g/L of sucrose, 1 g/L of activated carbon and 0.6 percent of agar, with a pH of 5.2-5.4; a culture medium for the proliferation and differentiation comprises 1/2 of MS, 0.5-2.0 mg/L of 6-BA, 0.2 mg/L of NAA, 2 g/L of tryptone, 15 percent (V/V) of coconut milk, 20 g/L of sucrose, 1 g/L of activated carbon and 0.6 percent of agar, with a pH of 5.2-5.4; and a culture medium for the sound seedling rootage comprises 1/2 of MS, 1.0 mg/L of NAA, 15 percent (in volume) of coconut milk, 20 g/L of sucrose, 1 g/L of activated carbon and 0.6 percent of agar, with a pH of 5.2-5.4. The method achieves over 45 percent of seed bourgeoning rate and over 90 percent of planting rate and transplantation survival rate.

The present invention relates to a low serum efficiency mycoplasma gallisepticam attenuated strain culture medium and a preparation method thereof, and belongs to the technical field of veterinary biology. The culture medium comprises: (1) a base culture medium; and (2) an auxiliary culture medium, wherein the auxiliary culture medium mainly comprises MEM, yeast extract powder, tryptone, glucose, an inorganic salt, and the like, and growth, high titer and stability of the semi-finished product can be ensured with the auxiliary culture medium. According to the present invention, a titer of the semi-finished product bacterial liquid prepared by using the preparation method is up to 10<11> CCU/ml; and the culture medium adopts reduced pig serum to culture mycoplasma gallisepticam so as to reduce allergic stress reactions on chicken by heterologous pig serum, consider animal biosafety, improve antigen titer, and reduce production cost.

The invention discloses a complex enrichment medium used for salmonella, listeria monocytogenes and staphylococcus aureus and a method for preparing the same. The complex enrichment medium comprises the following components in weight portion: 15 to 19 portions of tryptone, 2 to 4 portions of peptone, 2 to 3 portions of sodium dihydrogen phosphate, 2 to 3 portions of glucose, 0.5 to 1.5 portions of aesculin, 1, 000 portions of distilled water, 1 to 2.5 portions of sodium pyruvate, 3 to 10.0 portions of mannitol, 1.0 to 25.0 portions of sodium chloride, 1 to 2.5 portions of lithium chloride, 0.0001 to 0.0002 portion of potassium tellurite, and 0.005-0.015 portion of nalidixic acid, wherein the pH value is between 7.1 and 7.5. The complex enrichment medium can inhibit the growth of other pathogenic microorganisms while performing enrichment on three target pathogens, thus the complex enrichment medium can be directly applied to the isolation culture and the biological assay test of target bacteria, and can also be directly applied to a detection technology of a plurality of pathogens based on one detection platform for multiple PCR and the like to make a diagnostic report.

The invention relates to a clear liquid fermentation medium for clostridium butyricum. The clear liquid fermentation medium, the pH value of which is 7-8, is made from glucose, tryptone, a yeast extract powder, ammonium sulfate, sodium bicarbonate, a maize liquid powder and the like. The clear liquid fermentation medium for clostridium butyricum contains metal salts for promoting the growth of gemma, wherein the metal salts are manganese sulfate, magnesium sulfate and ferrous sulphate. The enrichment medium of the clear liquid fermentation medium for clostridium butyricum contains yeast extract, beef extract, tryptone, glucose, soluble starch, sodium chloride, sodium acetate trihydrate, cysteine hydrochloride, methylene blue at the concentration of 0.5% and distilled water; and the pH is adjusted to 7.1. A fermentation culture method of the clear liquid fermentation medium for clostridium butyricum comprises the following steps of: glycerin tube refrigerated strain activation, heating and optimization, Erlenmeyer flask first-order seed culture, liquid fermentation and spray drying. The production of clostridium butyricum has advantages of simple operation, high amount of zymocyte, high gemma yield, simple post-treatment, less impurities in a bacterial powder sample and high amount of effective live bacteria, and saves cost at large.

The invention relates to a nourishment fodder for honey bees, which comprises soybean flour 100 parts, yeast powder 50-70 parts, tryptone 5-15 parts, bee honey 70-90 parts, water 30-50 parts, white sugar 10-30 parts, whole milk powder 3-5 parts, composite vitamin 1-3 parts and lypressin 0.5-1.5 parts.

The invention relates to an efficient mycoplasma hyopneumoniae culture medium and a preparation method thereof, and belongs to the technical field of veterinary biology. The efficient mycoplasma hyopneumoniae culture medium comprises an A liquid and a B liquid mainly consisting of MEM, yeast leaching powder, tryptone, glucose, inorganic salt and the like. The prepared culture medium of the invention has the main advantages of low serum content which is only 10%-15%, while the serum content in common culture medium is 20% even more. The culture medium prepared by the low serum relieves the pig allergic to the stress reaction, meanwhile gives consideration to the biosafety of animals. Besides the valence of the semi-finished bacterial solution prepared by the method is up to 109CCU/ml, which is much higher than the culture medium prepared by the common technology.

Particular aspects provide novel compositions and methods useful for the growth, isolation and detection of microorganisms that have α-glucosidase activity (e.g., the bacterium E. sakazakii). Certain embodiments provide a novel growth and/or plating media, comprising a fluorogenic α-glucosidase substrate, which is both selective for and differential to E. sakazakii. In particular embodiments, the α-glucosidase substrate comprises 4-methylumbelliferyl-α-D-glucoside. Additional embodiments relate to a selection media. Further embodiments relate to a selective medium that is based on Tryptone Bile agar. Still further embodiments relate to OK media as defined herein. Other embodiments of the invention relate to methods for growing bacterial cultures on media that is selective for and differential to microorganisms that have α-glucosidase activity (e.g., the bacterium E. sakazakii).

The invention provides a heatproof lyoprotectant for a live vaccine against pseudorabies. The heatproof lyoprotectant comprises, by weight, 3 to 10 parts of gelatin, 1 to 5 parts of trehalose, 5 to 15 parts of sucrose, 0.1 to 2 parts bovine serum albumin, 1 to 8 parts of tryptone, 2 to 10 parts of enzyme-hydrolyzed casein, 1 to 5 parts of thiourea, 0.8 to 2 parts of L-monosodium glutamate, 0.1 to 3 parts of arginine, 0.5 to 5 parts of polyvinylpyrrolidone (PVP-K30) and 0.1 to 2 parts of mannitol. The invention also discloses a preparation method of the heatproof lyoprotectant and a lyophilized vaccine prepared from the heatproof lyoprotectant. When the heatproof lyoprotectant is used for protecting the vaccine and a specific lyophilization process is employed, lyophilization loss of viruses can be effectively reduced, the temperature tolerance of the viruses can be improved, and the vaccine can still maintain good physical properties and titer after long-term storage; i.e., the vaccine has stable characters and has the characteristics of heat resistance and long storage time.

The invention relates to the field of safety monitoring of food microorganisms, and discloses a salmonella characteristic chromogenic liquid nutrient medium, a preparation method thereof and a rapid detection method of salmonella. The salmonella characteristic chromogenic liquid nutrient medium comprises the following main components: tryptone, yeast powder, sodium chloride, lithium chloride, sodium deoxycholate, dipotassium phosphate, combined inhibitor, combined accelerator, characteristic enzymolysis substrate and cosolvent. The rapid detection method disclosed by the invention comprises two steps, i.e. pre-enrichment culture and chromogenic identification, and is characterized in that salmonella characteristic enzyme hydrolyzes corresponding substrates to result in that the culture medium is purple, thus rapidly judging the existence of salmonella; and the addition of the accelerator contributes to recovering damaged cells of salmonella and promoting growth of salmonella; and the added inhibitor can selectively inhibit the growth of other competitors so as to reduce the interference on detection by the competitors. The detection method disclosed by the invention has the advantages of short detection period, strong specificity and high accuracy, is simple to operate and is suitable for large-throughput detection of salmonella in food.

The invention discloses a haemophilus parasuis culture medium. A preparation method of the haemophilus parasuis culture medium comprises the steps of preparing a basic culture solution, treating coenzyme A, and perfecting a culture medium, wherein the basic culture solution comprises peptone, tryptone, sodium chloride, dextrose, yeast extract, glycerin and distilled water, and the basic culture solution, the coenzyme A and fetal bovine serum are uniformly mixed so as to obtain the haemophilus parasuis culture medium. The haemophilus parasuis culture medium provided by the invention can keep haemophilus parasuis, thereby facilitating the transportation of suspicious haemophilus parasuis samples; and the death of haemophilus parasuis is not caused in the process of transportation, thereby increasing the separation probability of the haemophilus parasuis.

The invention relates to a method of using wheat straws for producing bacterium cellulose, which includes the following steps: wheat straws are milled into 40 to 80 meshes and immersed by dilute sulphuric acid or hydrochloric acid in a reaction container according to the solid/liquid ratio of 1:6 to 1:30 of the wheat straws to the dilute acid liquid, the wheat straws react with the dilute sulphuric acid or hydrochloric acid at the temperature of 100 DEG C to 121 DEG C, the residual slag of the wheat straws and the acid hydrolyzed liquid are separated by sucking filtration, and the hydrolyzed liquid is collected and refrigerated for spare; the hydrolyzed liquid is detoxified; the detoxified hydrolyzed liquid is used as the carbon source of culture medium, 0.1 percent to 1 percent of yeast concrete and 0.1 percent to 0.5 percent of tryptone are added to be prepared into culture medium, the acetobacter or the glucose oxidized bacilli is vaccinated with the vaccinating amount of 6 percent to 10 percent to be cultivated in an oscillating table of 36 to 30 DEG C and 160 to 250 r/m or statically cultivated in an incubator of 26 DEG C to 30 DEG C, and the bacterium cellulose is obtained. The carbon source of culture medium prepared by the method has good quality and low price, and can be used for industrial production.

The invention discloses a method for preparing high-activity nattokinase by taking black beans as raw material, belonging to the technical field of biology. The method comprises the following steps: soaking the black beans by using a special nutrient solution, cooking the black beans after soaking and further inoculating bacillus natto to perform fermentation, wherein the special nutrient solution comprises the following components: lactose, tryptone, Na2HPO4, NaH2PO4, MgSO4 and CaCl2. The method further comprises the steps of performing extraction separation and freeze-drying on a fermentation product. The nattokinase obtained by adopting the method has high enzyme activity, which can achieve 42500IU/g, and the nattokinase has the advantages of high enzyme activity, low ammonia smell and good taste in comparison with the nattokinase produced by taking yellow beans as the raw material and performing a traditional fermentation method; and the content of nutritional components in obtained fermented black bean residue is also obviously improved. The method has the advantages of short fermentation period, simple preparation process and high bioavailability, and is conductive to industrial production.

The invention discloses a selective culture medium used for the quantitative detection of bacillus. Each litre of the selective culture medium comprises the following components: 1-10g of glucose, 1-5g of yeast extract, 5-10g of tryptone, 0.2-2g of calcium chloride, 0.1-1g of magnesium sulfate, 15-20g of agar and 100-500IU of bacteriostatic agent. Through variable temperature cultivation, the selective culture medium adopts can be used to increase the selectivity of the conventional plate counting method to bacillus, eliminate the influences of other microorganisms or impurities in s sample to be detected and achieve the aim of detecting bacillus fast and quantitatively.

The invention discloses a solid-state freeze-dried product of a quantitative strain and a preparation method and a using method thereof. 0.1% to 0.5% of glucose, 5% to 10% of sucrose, 0.5% to 1.5% of gelatin, 1% to 2% of tryptone, 0.5% to 1% of soy peptone, 0.5% to 1% of sodium chloride, 0.2% to 0.5% of yeast powder and 0.2% to 0.5% of activated carbon are prepared into solution serving as a protective agent, and after the solution and diluted bacteria suspension are uniformly mixed according to a certain proportion, the mixture is prepared into the dried product by freezing-drying; the product is excellent in solubility and can be completely dissolved in a dissolving agent in 1 to 2 seconds. The cylindrical freeze-dried product has an amount of bacteria of 100cfu to 1,000cfu or can customize bacterium content in a specific range. One single freeze-dried product is taken and dissolved into N ml of dissolving agent, and the bacterium content per N tenths ml is smaller than 100cfu, so that the solid-state freeze-dried product is accurate and convenient. Use of the protective agent can greatly reduce a damage degree of the strain in the freeze-drying process; the proper protective agent provides an excellent shape and freeze-drying environment for the product so as to guarantee activity of the strain to be protected to the greatest degree. The dissolving agent provides an excellent pH value, an excellent osmotic pressure and proper nutrient substances to the strain so as to guarantee the strain to obtain the optimal recovery conditions after the strain is dissolved.

The invention provides a culture medium used for separating and screening lactic acid bacteria. The formula of the culture medium comprises the following components: relative to the dosage of 1000ml of a solvent, 5.0-15.0g of tryptone, 5.0-15.0g of beef extract, 2.0-10.0g of yeast extract, 1.0-3.0g of diammonium citrate, 15.0-25.0g of glucose, 0.2-2.0 mL of Tween-80, 2.0-8.0g of sodium acetate, 15.0-20.0g of agar, 2.0-30.0g of calcium carbonate, a compound acid-base indicator containing 0.01-0.5g of methyl red and 0.01-0.7g of bromothymol blue, 0.5-5.0g of ascorbic acid, 0.1-0.5g of L-cysteine HCl, and a selective antibiotic composed of 250000-500000 IU of polymyxin B and 0.01-0.05g of nalidixic acid, wherein the pH value of the culture medium is 6.8; and the solvent is composed of a primary solvent and a secondary solvent, the primary solvent is distilled water or aged seawater, and the secondary solvent is saline solution. The culture medium has the characteristics of being high in accuracy, obvious in authentication phenomenon and the like during separation and screening for lactic acid bacteria.

This invention provides one collection and testing sequence for protein end enriching and testing method and test case, which comprises the following steps: protein end amidogen base decorating, alkali restoring base and tryptone enzyme slices, end peptide enrich end and mass spectrum test sequence. The invention through enzyme slice peptide mixture and end peptide section enriching and testing to get the protein end sequence information for protein testing and end analysis for protein group study. This invention also describes this method agent case.

The invention provides a butyric acid bacterium fermentation culture medium and a preparation method thereof and a butyric acid bacterium culture fermentation method. The butyric acid bacterium culture medium includes a nitrogen source, a carbon source and sodium chloride, wherein the nitrogen source is one or any combination of fish meal, bean pulp or yeast extract, and the nitrogen source is glucose and/or corn flour. The number of butyric acid bacteria can stably achieve more than 109CFU/mL after the fermentation culture medium is adopted for fermentation for 14-16h; according to the fermentation culture medium, the nitrogen source and the carbon source which are widely available are used for replacing tryptone, glucose and the like; the fermentation cost is low, the fermentation time is shortened, the fermentation efficiency is improved, and the production cost is greatly lowered.

The invention discloses bifidobacterium bifidum and application thereof. A preservation number of the bifidobacterium bifidum B-176 is CGMCC (China General Microbiological Culture Collection Center) No.15754, the preservation date is May 14, 2018, and the preservation institution is CGMCC. A formula of a culture medium of the bifidobacterium bifidum B-176 is characterized in that the culture medium is prepared from 1.2 to 2 percent of tryptone, 0.8 to 1.5 percent of soybean peptone, 0.8 to 1.6 percent of casein peptone, 0.5 to 1 percent of yeast extract powder, 1.0 to 1.5 percent of beef extract, 1.5 to 2 percent of glucose 6-phosphate, 1 to 2 percent of lactose and 4 to 4.5 percent of inorganic salt solution. The bifidobacterium bifidum B-176 disclosed by the invention has tolerance on gastric acid and cholate and has a better inhibition function on Escherichia coli; an experiment verifies that a strain has a better synergistic growth function with other types of lactobacillus, and anapplication range of the bifidobacterium bifidum B-176 is enlarged by the discovery. The invention also discloses the application of a lactobacillus composition containing the bifidobacterium bifidumB-176 in feed for fattening pigs. The lactobacillus composition can be added in original basic ration of the growing and fattening pigs for feeding, so that the utilization rate of the feed can be increased, the death rate can be reduced, and the slaughtering days can be reduced.

The invention discloses a method for preparing a clostridium butyricum feed additive and a clostridium butyricum feed additive prepared by the method. The method for preparing the clostridium butyricum feed additive is characterized by adopting clostridium butyricum (the clostridium butyricum is preserved in the China Center of Industrial Culture Collection and the serial number is CICC 20036). The method comprises the following steps: such materials as tryptone, beef extract, yeast cream, glucose, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, magnesium sulphate, calcium chloride, ferrous sulfate, cysteine hydrochloride and the like are prepared into a dedicated liquid culture medium for seeds; strains are grafted into the culture medium for activating seeds; nitrogen source, carbon source and the like are prepared into a solid anerobic fermentation culture medium; and the seed liquid is grafted into the solid anerobic fermentation culture medium for solid state fermentation and then is dried and sieved. By utilizing the cheap nitrogen and carbon sources to form the medium, the invention provides a novel method for large-scale and industrial production of clostridium butyricum; and the method reduces the cost effectively and increases the benefits of plants.

The present invention is one kind of nematode eating fungi capable of killing nematode and its preparation process and application, and belongs to the field of microbial pesticide technology. The nematode eating fungus is obtained through liquid fermentation of the strain, extracting the effective nematode killing component from the metabolic product and subsequent preparation step. The nematode eating fungus strain is Dactylella shizishanna YMF1.00022, and the liquid fermentation culture medium consists of gelatin, tryptone, yeast leaching powder, ammonium sulfate, ferrous sulfate, magnesium sulfate, disodium hydrogen phosphate, sodium dihydrogen phosphate and water in certain proportion. The extracting process of the effective nematode killing component includes taking supernatant from the fermented matter, adding ammonium sulfate, centrifuging, dissolving in buffering phosphoric acid solution, dialysis and other steps.

The invention provides a mycoplasma anatis culture medium. The mycoplasma anatis culture medium is composed of a liquid culture medium and a solid culture medium. The liquid culture medium is prepared from deionized water, a 10% thallium acetate solution, PPLO broth, glucose, peptone, tryptone, 1% phenol red, a CMRL-1066 culture medium containing L-glutamine, inactivated horse serum, yeast leachate, penicillin, L-cysteine hydrochloride and nicotinamide adenine dinucleotide. Besides the situation that agar powder is added and phenol red is removed, other components of the solid culture medium are the same as those of the liquid culture medium. The culture medium is used for separating and purifying mycoplasma anatis, wherein a bacterial colony is separated from the solid culture medium, the separated bacterial colony is placed in the liquid culture medium to grow, then filtering, dilution and plate coating of liquid culture substances and purification of picked single colony multiplication are conducted, and the steps are repeated till purified mycoplasma anatis is obtained. The culture medium can promote growth of mycoplasma anatis, and the success rate of separation and purification of mycoplasma anatis is high.

The invention discloses staphylococcus aureus chromogenic medium and a test slip thereof. The chromogenic medium contains tryptone, yeast extract powder, beef extract powder, glucose, sodium pyruvate, glycine, sodium chloride, monopotassium phosphate, disodium hydrogen phosphate, a bacteriostatic agent mixture and two specific enzyme chromogenic substrates. The chromogenic medium and the test slip made by using the chromogenic medium can be used for quickly detecting staphylococcus aureus in a large-flux sample, are obvious in positive result color development, accurate in tested result, strong in specificity, high in flexibility, short in test period, simple and convenient to operate, low in test environment requirement and test personnel, and capable of carrying out quantitative analysis while carrying out qualitative test on the staphylococcus aureus, and therefore, the chromogenic media and the test slip are very suitable for being used by grass-roots supervisory authorities and enterprises, and are extensive in application prospect.

The invention provides a making method of a high-nutrition white fungus beverage having typical flavor of white funguses. According to the method, special mixed bacteria are used for fermentation. Themethod comprises the following steps of (1) preparing fermentation substrates, wherein the fermentation substrates consist of the following components in percentage by weight: 0.2% of food grade glucose, 0.1-0.2% of food grade casein tryptone, 0.05-1% of a yeast extract and the balance of a white fungus polysaccharide extraction solution; (2) sterilizing the fermentation substrates, inoculating the sterilized fermentation substrates with mixed bacteria, and performing vibrating fermentation culture at 32-35 DEG C for 3-7 days so as to obtain a fermentation solution; and (3) adding an appropriate quantity of food auxiliary materials to the fermentation solution obtained in the step (2) so as to obtain the high-nutrition white fungus beverage. The white fungus polysaccharide fermentation solution has typical white fungus flavor, contains rich functional nutrient components and contains various probiotics, so that functional requirements of people for a plant-derived beverage are met, and the flavor requirements of people for a plant-derived beverage are also met.