89 results about "Tryptone" patented technology

The invention relates to a selective chromogenic medium for separation and identification of streptococcus suis and a preparation method thereof, and belongs to the field of microbial culture. In the prior art, a streptococcus suis culture medium mainly focuses on increasing the content of streptococcus suis strains, but is not suitable for clinically separating and identifying the streptococcus suis, and other infectious microbes in a clinical sample are difficult to distinguish, so that separation fails. In order to overcome the defects in the prior art, the invention provides a selective chromogenic culture medium for separating and identifying streptococcus suis and a preparation method thereof, the culture medium comprises tryptone, soybean peptone and other components, and when the culture medium is used for separating pathogenic bacteria of a clinical porcine respiratory tract sample, the separation efficiency is greatly improved. The streptococcus suis can be accurately separated and identified according to the specific color development and growth state of the bacterial colony, the influence of other infectious microbes is remarkably reduced, and the method is suitable for being popularized and applied in the porcine respiratory tract sample treatment process.

The invention relates to the technical field of microorganisms, and discloses an induction method of VBNC state shigella, a resuscitation medium and application, and the resuscitation medium comprises Rpf recombinant protein, MgSO4, sodium pyruvate, vitamin B12, tryptone, glucose, dipotassium phosphate, monopotassium phosphate, sodium chloride and Tween 80. The resuscitation culture medium obtained by the invention can be used for successfully culturing Shigella in a VBNC state.

The invention discloses a long lysinibacillus inoculant, a preparation method and application, the inoculant is prepared from a long lysinibacillus strain C1, and the preparation method comprises the following steps: culturing the strain of the long lysinibacillus strain through a tryptone soybean agar culture medium until yellow single colonies appear; inoculating the yellow single colony into serum, and carrying out seed culture by using a long lysine bacillus liquid culture medium until a logarithmic phase to obtain a bacterial solution; and inoculating the cultured bacterial liquid into a production device according to a long lysine bacillus strain, and carrying out production culture by using a long lysine bacillus production culture medium to a logarithmic phase to prepare the long lysine bacillus biocontrol inoculant. And carrying out centrifugal treatment on the obtained biocontrol microbial inoculum of the long lysine bacillus, taking a supernatant, and filtering the supernatant to obtain the long lysine bacillus biocontrol fermentation broth. The invention further discloses application of the lysinibacillus elongate microbial inoculum in antagonism of rice blast bacteria and prevention and control of rice blast.

The invention discloses a bacterial culture medium for producing algin through liquid fermentation. The bacterial culture medium contains triclosan and SDS (Sodium Dodecyl Sulfate). Tests show that the PIB culture medium added with triclosan and SDS can improve the capability of producing algin by PFAC-PAO1 liquid fermentation. Accordingly, the inventor develops innovative research of optimizing the culture medium, the ChinaYin culture medium is finally obtained, and the specific formula of the ChinaYin culture medium is as follows: 20g / L of tryptone, 1.4 g / L of magnesium chloride, 10g / L of potassium sulfate, 20mL / L of glycerol, 50mu g / mL of triclosan and 125mu g / mL of SDS (Sodium Dodecyl Sulfate). Researches show that by applying the culture medium, the polysaccharide yield is higher than that of a PIB culture medium and a USAYu culture medium, the yield in 48 hours is higher than that of 24 hours and 72 hours, the yield of algin powder obtained in each culture bottle can reach 8 g / L, and it is indicated that the ChinaYin culture medium has the potential of being industrially amplified to be used for fermentation production of bacterial algin.

The present invention belongs to the technical field of rapid detection of drug sensitivity for bacteria. Disclosed are an enrichment culture medium, and a preparation method therefor and the use thereof. The enrichment culture medium comprises the following raw materials in parts by mass: 15.0-20.0 parts of tryptone, 3.5-5.5 parts of peptone, 1.5-3.5 parts of glucose, 5.0-6.5 parts of yeast extract, 2.0-2.5 parts of dipotassium phosphate, 2.0-3.5 parts of disodium hydrogen phosphate, 1.0-1.5 parts of monopotassium phosphate, 2.5-7.5 parts of sodium chloride, 12.0-18.0 parts of proteose peptone, 15.0-22.0 parts of beef heart infusion powder, 8.0-12.0 parts of soya peptone, 1.0-5.0 parts of 0.1% nicotinamide adenine dinucleotide (NAD), 30-100 parts of newborn calf serum inactivated at 56°C for 30 min, 2.0-4.0 parts of mannitol, 3.0-5.0 parts of sodium pyruvate, 0.23-0.45 parts of cycloheximide, 0.8-2.0 parts of 0.05% bromothymol blue, 2.5-4.5 parts of 0.1% methylene blue, and 1000 parts of distilled water, with the pH value of 6.8-7.5. The enrichment culture medium is used to screen effective therapeutic drugs for bacterial diseases in poultry, and is convenient to use and has a single enrichment effect superior to that of respective selective enrichment broths of target bacteria. Therefore, the culture medium can realize rapid proliferation of common pathogenic bacteria in poultry.

The invention discloses a culture medium for A type clostridium perfringens and C type clostridium perfringens, a fermentation culture method and application of fermentation toxin production. Wherein the culture medium comprises the following components: tryptone, # imgabs0 # peptone, casein peptone, a yeast extract, lactoalbumin hydrolysate, trihydroxymethyl aminomethane, sodium acetate and glucose. According to the present invention, through the specific formula composition, the A-type clostridium perfringens and the C-type clostridium perfringens can adapt to fermentation in the fermentation tank, and in the fermentation culture process, the toxin production ability of the A-type clostridium perfringens and the C-type clostridium perfringens can be significantly improved by controlling the stirring speed, the pH value of the fermentation liquid and the precise material supplementing manner. And the supernate obtained by fermentation is used as a raw material for preparing the vaccine, so that animals can generate high-level neutralizing antibodies aiming at the clostridium perfringens A and C type toxins after being immunized with the vaccine, the death of the animals caused by infection of the clostridium perfringens A and C type toxins is avoided, and the method has practical popularization and application values.

Disclosed in the present invention are a Serratia marcescens strain, and the use thereof in co-production of prodigiosin, chitinase and lipase. The Serratia marcescens strain is classified and named Serratia marcescens LT-7 and has been preserved in China Center for Type Culture Collection with the accession number CCTCC NO: M20231646, the preservation date being September 07 2023. The strain synthesizes prodigiosin, chitinase and lipase by means of fermentation by using molasses as a carbon source and tryptone as a nitrogen source; and during a coproduction process, the concentration of prodigiosin accumulated in a culture medium reaches up to 20.70 g / L, the activity of chitinase is 49.78 U / mL and the activity of the lipase is 86.92 U / mL. The operation method disclosed by the present invention is simple, effective and low-cost, has a great industrial production prospect, and provides an innovative process for the biosynthesis of prodigiosin, chitinase and lipase.

The invention discloses a vaccine freeze-dried heat-resistant protective agent and a preparation method and application thereof. The freeze-dried heat-resistant protective agent consists of the following components in weight percentage: 0.6-1.0% of gelatin, 4.5-6.5% of brain heart infusion, 3.5-5.5% of tryptone, 6-10% of trehalose, 2.0-3.0% of sucrose, 1.5-2.5% of sodium thiosulfate, 0.6-1.2% of polyvinyl pyrrolidone (PVPK30), 0.4-0.6% of dextran, 0.3-0.5% of mannitol, 0.2-0.4% of glycine, 0.04-0.06% of sodium glutamate, 0.2-0.3% of L-arginine, and the balance is water for injection. The freeze-dried heat-resistant protective agent of the vaccine disclosed by the invention has components that interact with each other, thereby improving the temperature tolerance of porcine infectious gastroenteritis, porcine epidemic diarrhea and porcine rotavirus triple live vaccines during freeze-drying, reducing the loss of efficacy, and the virus loss rate before and after freeze-drying does not exceed 0.2 titers.

The application discloses a fabric yellowing reagent and a fabric yellowing method, and relates to the technical field of daily chemical products. 5 The fabric yellowing reagent comprises 1-3% of histidine solution and a bacterial suspension with a bacterial concentration of 1*10 5 CFU / mL-9*10 The bacteria are a mixture of one or more of Staphylococcus epidermidis and Micrococcus luteus; the solvent of the histidine solution is normal saline containing 0.15-1% of trypsin peptone; and the solvent of the bacterial suspension is normal saline containing 0.1-0.2% of trypsin soybean peptone liquid culture medium. The fabric yellowing reagent can be used for simulating fabric yellowing, and has good operability, reproducibility and data reliability.

The invention relates to the technical field of Escherichia coli detection, and particularly discloses a semisolid culture medium for rapid detection of Escherichia coli and a detection method and application thereof, the semisolid culture medium comprises the following components by weight: 4.2-5.8 g / L of agar; 8.5 to 11.5 g / L of tryptone; the semi-solid culture medium comprises the following components: 3.2 to 4.8 g / L of beef extract, 6.5 to 9.5 g / L of lactose, 1.2 to 2.2 g / L of D-glucose, 4.2 to 5.8 g / L of sodium chloride, 42000 to 58000 [mu] g / L of penicillin, 0.12 to 0.28 g / L of cholate, 0.06 to 0.14 g / L of disodium EDTA (Ethylene Diamine Tetraacetic Acid), 0.12 to 0.22 g / L of L-cysteine, 1.2 to 2.8 g / L of yeast extract, 0.005 to 0.015 g / L of vitamin B and an acid-base indicator, the semisolid culture medium and the detection method thereof provided by the invention not only solve the core pain point of the existing escherichia coli detection method in the technical level, but also reduce the operation cost and improve the benefit for enterprises in the economic level, promote the standardization and high efficiency of the food microorganism detection technology in the industry level, and have practicability and industrial popularization value.

The invention discloses a culture medium for colonization of intestinal flora and intestinal gas of gastrointestinal cancer crowds. The culture medium contains yeast extract, tryptone, mucoprotein, mycoprotein, insect protein, soybean protein, fructo-oligosaccharide, galactooligosaccharide, inulin, resistant dextrin, dietary fiber, porridge, vegetable juice, fruit juice, oat powder, coix seed powder, black bean powder, sialic acid, algal oil DHA, potassium chloride, sodium chloride, monopotassium phosphate, sodium sulfate, bile salt and a plant extract mixture. Vitamin mixed liquid and the like. By means of the bionic colon reactor, by referring to real in-vivo parameters of gastrointestinal cancer people, the real intestinal environment of domestic gastrointestinal cancer people is well simulated in vitro, the intestinal flora ecology and intestinal gas distribution are reproduced, and a normal intestinal microorganism and intestinal gas database of the gastrointestinal cancer people can be constructed. The method is beneficial to accurate diagnosis and personalized treatment of intestinal microecology of people with gastrointestinal cancer, and better evaluates the health state of people with gastrointestinal cancer and formulates preventive measures.

The application discloses a virus-like particle vaccine freeze-drying protective agent and a preparation method thereof. The freeze-drying protective agent comprises A liquid and B liquid mixed in a volume ratio of 1:1 to 3:1; the A liquid comprises the following components with terminal concentrations: 0.01-0.5 mol / L base liquid I, 0.5-2 mmol / L base liquid II, 0.01-0.1% polysorbate 80, 5-20% sucrose and 1-5% trypsin; and the B liquid comprises the following components with terminal concentrations: 0.1-5% trehalose, 0.1-5% oligogalactose and 0.1-2% PEG6000. Through the compounding of the A liquid and the B liquid, the application can effectively enhance the immunization effect of the vaccine, prolong the storage time of the vaccine and keep the virus-like particle form intact.

The invention belongs to the technical field of microorganisms, and discloses a high-density fermentation process of vagina-sourced lactobacillus crispatus. The invention discloses a fermentation medium which comprises glucose, tryptone, beef extract, yeast extract, dipotassium phosphate, ammonium citrate, L-cysteine or salt thereof, magnesium sulfate and manganese sulfate. According to the fermentation culture medium provided by the invention, through scientific compounding of a carbon source, a nitrogen source and key factors, the final viable concentration and thallus activity of vagina-derived lactobacillus crispatus can be remarkably improved. The fermentation culture medium is adaptive to the micro-aerobic metabolism characteristics of vagina-derived lactobacillus crispatus by precisely regulating and controlling the dissolved oxygen level (initial dissolved oxygen is controlled to be 5% or below), and when the fermentation culture medium is used for culturing lactobacillus crispatus, the fermentation delay period can be remarkably shortened to 2-3 h, the final viable bacterium yield is greatly increased to 1 * 10 < 10 > CFU / mL or above, and the production cost is reduced. And an efficient and stable technical support is provided for industrial production of the probiotic preparation.

The invention discloses a culture medium for colonization of intestinal flora and intestinal gas of people with hyperuricemia. The culture medium contains yeast extract, ethanol, adenine, tryptone, mucoprotein, mycoprotein, insect protein, soybean protein, fructose, glucose, inulin, resistant dextrin, dietary fiber, oxalic acid, saturated fatty acid, sialic acid, peanut oil, potassium chloride, sodium chloride, monopotassium phosphate, sodium sulfate, bile salt, cysteine hydrochloride and a plant extract mixture. Vitamin mixed liquid and the like. By means of the bionic colon reactor, by referring to real in-vivo parameters of people suffering from hyperuricemia, the real intestinal environment of people suffering from hyperuricemia in China is well simulated in vitro, the intestinal flora ecology and intestinal gas distribution are reproduced, and a normal intestinal microorganism and intestinal gas database of people suffering from hyperuricemia can be constructed. The method is beneficial to accurate diagnosis of intestinal microecology of people with hyperuricemia, individualized treatment of antisense booking and disease mechanisms, better maintenance of intestinal health and formulation of preventive measures.

The application discloses a composition capable of effectively inhibiting the growth of Staphylococcus aureus biofilm. The application firstly optimizes the nitrogen source in the culture medium of Staphylococcus epidermidis CCSM0287 strain, fully explores the growth difference of Staphylococcus epidermidis under different culture media and culture conditions and the inhibition effect of the fermentation supernatant on Staphylococcus aureus biofilm, then compounds the fermentation supernatant of Staphylococcus epidermidis CCSM0287 obtained by using the optimized culture medium (the nitrogen source adopts tryptone + soybean peptone + fresh beef bone collagen peptide powder) with the fermentation supernatant of Lactobacillus paracasei S20, can obviously inhibit the growth of Staphylococcus aureus biofilm and destroy the surface structure of the Staphylococcus aureus biofilm, and can be applied in bacteriostatic products.

The invention relates to a fermentation medium of porcine circovirus type 3 (PCV3) Cap protein virus-like particles and a fermentation method thereof. The fermentation culture medium comprises tryptone, a yeast extract, casein hydrolysate, sorbitol, glycerol, polyoxyethylene mono-tert-octyl phenyl ether and the like. Methanol is supplemented for three times in the fermentation process, so that the expression efficiency of the Cap protein of PCV3 is remarkably improved, and correct folding and self-assembly of the Cap protein into the virus-like particles are promoted. According to the culture medium and the method, generation of impure protein is reduced, the purification cost is reduced, and key technical support is provided for industrial production of PCV3 subunit vaccines.

The invention relates to a preparation method of a bacterial cellulose composite nano-silver biological dressing, and belongs to the technical field of biological material preparation. Enzymatic hydrolysate of bean pulp, alfalfa leaching liquor and tryptone are used as fermentation culture media, acetobacter xylinum seed liquid is added, the bacterial cellulose membrane is obtained, and the content of the bacterial cellulose membrane composite nano-silver biological dressing is limited to 50-99% of water. Comprising chitosan, gamma-polyglutamic acid, beta-glucan, refined water and silver ions. The bacterial cellulose membrane composite nano-silver biological dressing has the effects that the bacterial cellulose membrane composite nano-silver biological dressing is high in mechanical strength, high in water-retaining property, good in biocompatibility, good in exudate absorption capacity and non-toxic, and as the nano-silver biological dressing for wounds, the cellulose composite nano-silver biological dressing can provide a moist environment, is beneficial to rapid cleaning of the wounds, and has a good application prospect. Meanwhile, the antibacterial activity of chitosan, gamma-polyglutamic acid, beta-glucan, silver ions and the like on bacteria is more beneficial to wound healing.

The invention relates to a method for preparing thiogambogic bacteria by utilizing a streptomyces deserticola new strain, which comprises the following steps: (1) activating: inoculating a yeast starch solid culture medium with the streptomyces deserticola new strain Streptomyces sp. HMX112 preserved at-80 DEG C in a streak manner, and culturing at 30 DEG C for 14 days to obtain activated pure culture strain HMX112 spores; (2) seed culture: inoculating activated pure culture strain HMX112 spores into a casein tryptone liquid culture medium, and performing shake cultivation to obtain a first-stage seed solution; (3) fermentation culture: inoculating the first-grade seed liquid into a Gause No.1 liquid fermentation culture medium, and carrying out shake culture to obtain fermentation liquid; (4) fermentation liquor treatment: pre-cooling the fermentation liquor at 4 DEG C, then carrying out solid-liquid separation, and repeatedly extracting and concentrating the obtained supernatant for more than three times by using isometric ethyl acetate to obtain a crude extract; and (5) preparing the sulfur garcinia. The preparation method is simple, and the sample purity is high.

The invention relates to the technical field of microorganisms, and discloses an improved TSA bouillon culture medium and application thereof.The improved TSA bouillon culture medium comprises tryptone, sodium chloride, soybean peptone, agar, Rpf recombinant protein and magnesium sulfate, and the mass concentration ratio of tryptone to sodium chloride to soybean peptone to agar to Rpf recombinant protein to magnesium sulfate is (10 g / L-20 g / L) to (4 g / L-6 g / L) to (3 g / L-7 g / L) to (13 g / L-17 g / L) to (10 mu g / L-100 mu g / L) to (0.5 g / L-0.7 g / L). The improved TSA broth culture medium obtained by the invention can be used for successfully culturing Escherichia coli in a VBNC state and staphylococcus aureus in the VBNC state.

The invention relates to a culture medium for separating and identifying actinobacillus pleuropneumoniae in a pig clinical disease material and a preparation method of the culture medium, and belongs to the field of microbial culture. In the prior art, most of culture media for separating actinobacillus pleuropneumoniae in porcine clinical respiratory tract infection materials are conventional common TSA or improved TSA culture media, so that the actinobacillus pleuropneumoniae is difficult to distinguish from other respiratory tract infection bacteria, and the culture media are not suitable for clinically separating and identifying the actinobacillus pleuropneumoniae. In order to overcome the defects in the prior art, the invention provides the chromogenic culture medium for separating and identifying actinobacillus pleuropneumoniae in the clinical pig disease material and the preparation method of the chromogenic culture medium, and the culture medium comprises tryptone, soy peptone and other components. Actinobacillus pleuropneumoniae can be separated and identified according to the specific color development and growth state of bacterial colonies, the influence of other infectious microbes is remarkably reduced, and the method is suitable for being popularized and applied in the disease sample treatment process after porcine respiratory tract infection.

The invention discloses a microecological preparation for improving reproductive performance of sows and a preparation method of the microecological preparation, and relates to the technical field of microorganism application. Comprising the following steps: activating and fermenting clostridium butyricum to obtain bacterial sludge; mixing the bacterial sludge with tryptone, glucose, L-cysteine and fructose to obtain an inner-layer mixture, and granulating to obtain inner-layer mixture particles; preparing chitosan-coated colloid, soaking the inner-layer mixture particles in the chitosan-coated colloid, taking out the inner-layer mixture particles, and freeze-drying the inner-layer mixture particles to obtain a barrier film outside the inner-layer mixture; and coating the outer surface of the barrier film with outer-layer powder to obtain the microecological preparation. According to the preparation method, bacterial sludge of clostridium butyricum is coated with a barrier film and outer-layer powder, and the microecological preparation which is used for feeding sows and carrying out clostridium butyricum intervention on pregnant sows is prepared; after the sow is fed with the feed mixed with the microecological preparation prepared by the invention, the reproductive capacity of the sow is obviously improved, and the growth and development of the piglet are also obviously promoted.

The invention relates to the technical field of biological medicines, and particularly discloses a culture medium for high-density expression of an FMDV 3ABC antigen by escherichia coli and a fermentation culture method. The invention discloses a culture medium for high-density expression of an FMDV 3ABC antigen by escherichia coli. The culture medium comprises a basic culture medium and a fed-batch culture medium, the basic culture medium is prepared from tryptone, a yeast extract, glycerol, a stabilizer, ampicillin sodium, NaCl, K2HPO4, MgSO4 and CaCO3; the solvent is water; the fed-batch culture medium is prepared from glucose, peptone, yeast extract, glycerol, ammonium sulfate, a stabilizer and ampicillin sodium; the solvent is water; the stabilizer is prepared from polydimethylsiloxane aqueous emulsion and palmarosa oil. By utilizing the technical scheme provided by the invention, the fermentation culture time in the fermentation culture method for expressing the FMDV 3ABC antigen by escherichia coli can be shortened, and the obtained antigen product is high in yield and high in purity.

The invention discloses a culture medium for realizing selective enrichment of salmonella. The culture medium is prepared from a tryptone soybean broth culture medium, sodium citrate, deoxysodium cholate, distilled water and a 0.1% brilliant green aqueous solution. The invention also discloses a preparation method of the culture medium for realizing selective enrichment of salmonella, which comprises the following steps: S1, adding 30.0 g of tryptone soybean broth culture medium, 4.0 g of sodium citrate and 0.7 g of deoxysodium cholate in proportion, adding 1000mL of distilled water, heating and stirring in water bath until dissolving, sterilizing at 121 DEG C under high pressure for 15 minutes to obtain a semi-finished culture medium solution, and storing at normal temperature; and step S2, in a temporary use stage, aseptically adding 0.4 mL of 0.1% brilliant green aqueous solution into every 1000mL of semi-finished culture medium solution in a super clean bench environment, so as to prepare the culture medium for realizing selective enrichment of salmonella. The detection efficiency of pathogenic bacteria is effectively improved.

The invention relates to the technical field of microbial fermentation, and particularly provides a high-density fermentation method for improving immunogenicity of duck escherichia coli. The high-density basic culture medium is prepared from the following components: 2 to 15g / L of yeast extract, 5 to 20g / L of tryptone, 34 to 36g / L of KH2PO4, 1.6 to 2.0 g / L of (NH4) 2HPO4, 1.5 to 2.5 g / L of NaCl, 0.5 to 3g / L of citric acid, 0.5 to 2g / L of MgSO4, 2 to 20g / L of glucose and 0.01 to 0.1 g / L of VB1. And the high-density fed culture medium comprises 50-500g / L of glucose, 10-50g / L of yeast extract and 20-100g / L of tryptone. Compared with the prior art, large-scale high-density fermentation of duck Escherichia coli can be realized by using the two, and the immunogenicity of duck Escherichia coli can be improved.

The invention discloses a culture medium for poultry pasteurella multocida. The culture medium is prepared from the following components: 24g / L to 25g / L of tryptone, 18g / L to 20g / L of dipotassium phosphate heptahydrate, 7g / L to 8g / L of sodium chloride, 0.25 g / L of anhydrous magnesium sulfate, 1.36 g / L of monopotassium phosphate and 10g / L of glucose. The prepared culture medium for the poultry pasteurella multocida is applied to enrichment culture of the poultry pasteurella multocida, the culture medium suitable for the poultry pasteurella multocida can be obtained by optimizing the components and the component proportion of the culture medium, the culture medium is applied to the enrichment culture of the poultry pasteurella multocida, the obvious enrichment effect is achieved, and the culture medium is suitable for the poultry pasteurella multocida. The cost of the culture medium is obviously lower than that of a conventional culture medium or culture scheme.

The present disclosure provides a method for increasing the yield of Amuciniphila, the method comprising culturing Akkermansia muciniphila with a culture medium containing peptone or yeast extract powder, the peptone comprising at least one of soy peptone, tryptone, wheat peptone, or rice peptone, and the method comprising: culturing Akkermansia muciniphila with a culture medium containing yeast extract powder; according to the method disclosed by the invention, the yield of the active ingredient Amuc1100 protein of Ackermania muciniphila can be obviously improved.

The invention discloses a culture medium for colonization of intestinal flora and intestinal gas of infants. The culture medium contains a yeast extract, tryptone, mucoprotein, mycoprotein, worm protein, soybean protein, fructo-oligosaccharide, galactooligosaccharide, inulin, resistant dextrin, breast milk oligosaccharide, dietary fiber, sialic acid, algal oil DHA, potassium chloride, sodium chloride, monopotassium phosphate, sodium sulfate, bile salt, a plant extract mixture, a vitamin mixed solution and the like. By means of the bionic colon reactor, the real intestinal environment of domestic infants is well simulated in vitro by referring to real parameters in the infants, the intestinal flora ecology and intestinal gas distribution are reproduced, and a normal intestinal microorganism and intestinal gas database of the infants can be constructed. Accurate diagnosis and personalized treatment of the intestinal microecology of the infants are facilitated, the health state of the infants can be better evaluated, and preventive measures can be better formulated.

The invention discloses a culture method for promoting the growth of floating moss of amphibious moss plants by using nitrogen-fixing bacteria, which remarkably improves the growth speed and biomass of the floating moss of the amphibious moss plants by co-culturing the floating moss and the nitrogen-fixing bacteria so as to achieve a better growth promotion effect. The nitrogen-fixing bacteria disclosed by the invention are Rhodopseudomonas palustris, and the co-culture method comprises the following steps of: activating the Rhodopseudomonas palustris, and culturing the activated Rhodopseudomonas palustris in a tryptone soybean liquid culture medium. When the bacterial liquid is turbid and the O.D600 value is measured to be about 1, sucking the bacterial liquid on a culture medium containing 1 / 2GB5 and 1% of cane sugar, coating by using a coating device, and inoculating floating moss on a flat plate containing the bacterial liquid; the method provided by the invention can obviously promote the growth of floating moss biomass, and has a wide prospect in the direction of promoting plant growth.

The invention relates to the technical field of microorganisms, and discloses an improved LST broth culture medium and application, and the improved LST broth culture medium comprises tryptone, ammonium chloride, lactose, dipotassium phosphate, monopotassium phosphate, sodium lauryl sulfate, Rpf recombinant protein, magnesium sulfate, sodium pyruvate and pyridoxal phosphate. The improved LST broth culture medium disclosed by the invention can be used for successfully enriching the VBNC-state escherichia coli, so that researchers can successfully detect the VBNC-state escherichia coli in a culture manner.

The invention discloses a culture medium for colonization of intestinal flora and intestinal gas of middle-aged and elderly people. The culture medium contains yeast extract, tryptone, mucoprotein, mycoprotein, worm protein, soybean protein, fructo-oligosaccharide, galactooligosaccharide, inulin, resistant dextrin, dietary fiber, sorghum, buckwheat, oat, coix seed, corn germ oil, olive oil, linseed oil, walnut oil, sialic acid, algal oil DHA, potassium chloride, sodium chloride, monopotassium phosphate, sodium sulfate and bile salt. A plant extract mixture, a vitamin mixed solution and the like. By means of the bionic colon reactor, the real intestinal environment of the middle-aged and elderly people in China is well simulated in vitro by referring to real in-vivo parameters of the middle-aged and elderly people, the intestinal flora ecology and intestinal gas distribution of the middle-aged and elderly people are reproduced, and a database of normal intestinal microorganisms and intestinal gas of the middle-aged and elderly people can be constructed; accurate diagnosis and personalized treatment of intestinal microecology of the middle-aged and elderly people are facilitated, and the health state of the middle-aged and elderly people can be better evaluated and preventive measures can be better formulated.