Low-serum culture medium for efficiently culturing mycoplasma hyopneumoniae and preparation method thereof
A technology of Mycoplasma hyopneumoniae and culture medium, which is applied in the field of low serum and high-efficiency culture Mycoplasma hyopneumoniae culture medium and its preparation field, can solve the problems of increasing allergic stress response of pigs, low titer of viable bacteria, affecting the immune efficacy of vaccines, etc. The effect of reducing allergic stress response and increasing the titer of viable bacteria
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0023] 1, the preparation of culture medium of the present invention
[0024] Basal medium:
[0025]
[0026] Sterilize at 116°C for 30 minutes. After cooling, add the following auxiliary medium components under aseptic conditions to make a medium for culturing Mycoplasma hyopneumoniae.
[0027] Auxiliary medium:
[0028]
[0029]
[0030] Dissolve the components of the basal medium (1)-(8) one by one into 500ml distilled water for high-pressure sterilization, add auxiliary medium after cooling, and adjust the pH to 7.5 with 1mol / L sodium hydroxide solution to obtain low-serum and high-efficiency Mycoplasma hyopneumoniae culture base.
Embodiment 2
[0032] 1, the preparation of culture medium of the present invention
[0033] Basal medium:
[0034]
[0035] Sterilize at 116°C for 30 minutes. After cooling, add the following auxiliary medium components under aseptic conditions to make a medium for culturing Mycoplasma hyopneumoniae.
[0036] Auxiliary medium:
[0037]
[0038] Dissolve the components of the basal medium (1)-(8) one by one into 800ml distilled water for high-pressure sterilization, add auxiliary medium after cooling, and adjust the pH to 7.6 with 1mol / L sodium hydroxide solution to obtain low-serum and high-efficiency Mycoplasma hyopneumoniae culture base.
Embodiment 3
[0040] Using Mycoplasma hyopneumoniae to carry out comparative culture test on Mycoplasma hyopneumoniae through the Mycoplasma culture medium of the present invention, KM2 culture medium, PPLO Mycoplasma culture medium respectively.
[0041] 1, the preparation of culture medium of the present invention
[0042] Basal medium:
[0043]
[0044]
[0045] Sterilize at 116°C for 30 minutes. After cooling, add the following auxiliary medium components under aseptic conditions to make a medium for culturing Mycoplasma hyopneumoniae.
[0046] Auxiliary medium:
[0047]
[0048] Dissolve the components of the basic medium (1)-(8) one by one into 1000ml distilled water and sterilize under high pressure, add auxiliary medium after cooling, and adjust the pH to 7.6 with 1mol / L sodium hydroxide solution to obtain low-serum and high-efficiency Mycoplasma hyopneumoniae culture base.
[0049] 1. Mycoplasma hyopneumoniae strains and culture conditions
[0050] According to the cul...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com