Low-serum culture medium for efficiently culturing mycoplasma hyopneumoniae and preparation method thereof

A technology of Mycoplasma hyopneumoniae and culture medium, which is applied in the field of low serum and high-efficiency culture Mycoplasma hyopneumoniae culture medium and its preparation field, can solve the problems of increasing allergic stress response of pigs, low titer of viable bacteria, affecting the immune efficacy of vaccines, etc. The effect of reducing allergic stress response and increasing the titer of viable bacteria

Active Publication Date: 2013-04-24
兆丰华生物科技(南京)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The Mycoplasma hyopneumoniae cultured with the above-mentioned traditional culture medium and culture technology has poor reproductive ability and low titer of viable bacteria. In addition, the live vaccine prepared with excess serum will undoubtedly increase the allergic stress response of pigs, and ultimately affect the immune efficacy of the vaccine.

Method used

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  • Low-serum culture medium for efficiently culturing mycoplasma hyopneumoniae and preparation method thereof
  • Low-serum culture medium for efficiently culturing mycoplasma hyopneumoniae and preparation method thereof
  • Low-serum culture medium for efficiently culturing mycoplasma hyopneumoniae and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1, the preparation of culture medium of the present invention

[0024] Basal medium:

[0025]

[0026] Sterilize at 116°C for 30 minutes. After cooling, add the following auxiliary medium components under aseptic conditions to make a medium for culturing Mycoplasma hyopneumoniae.

[0027] Auxiliary medium:

[0028]

[0029]

[0030] Dissolve the components of the basal medium (1)-(8) one by one into 500ml distilled water for high-pressure sterilization, add auxiliary medium after cooling, and adjust the pH to 7.5 with 1mol / L sodium hydroxide solution to obtain low-serum and high-efficiency Mycoplasma hyopneumoniae culture base.

Embodiment 2

[0032] 1, the preparation of culture medium of the present invention

[0033] Basal medium:

[0034]

[0035] Sterilize at 116°C for 30 minutes. After cooling, add the following auxiliary medium components under aseptic conditions to make a medium for culturing Mycoplasma hyopneumoniae.

[0036] Auxiliary medium:

[0037]

[0038] Dissolve the components of the basal medium (1)-(8) one by one into 800ml distilled water for high-pressure sterilization, add auxiliary medium after cooling, and adjust the pH to 7.6 with 1mol / L sodium hydroxide solution to obtain low-serum and high-efficiency Mycoplasma hyopneumoniae culture base.

Embodiment 3

[0040] Using Mycoplasma hyopneumoniae to carry out comparative culture test on Mycoplasma hyopneumoniae through the Mycoplasma culture medium of the present invention, KM2 culture medium, PPLO Mycoplasma culture medium respectively.

[0041] 1, the preparation of culture medium of the present invention

[0042] Basal medium:

[0043]

[0044]

[0045] Sterilize at 116°C for 30 minutes. After cooling, add the following auxiliary medium components under aseptic conditions to make a medium for culturing Mycoplasma hyopneumoniae.

[0046] Auxiliary medium:

[0047]

[0048] Dissolve the components of the basic medium (1)-(8) one by one into 1000ml distilled water and sterilize under high pressure, add auxiliary medium after cooling, and adjust the pH to 7.6 with 1mol / L sodium hydroxide solution to obtain low-serum and high-efficiency Mycoplasma hyopneumoniae culture base.

[0049] 1. Mycoplasma hyopneumoniae strains and culture conditions

[0050] According to the cul...

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Abstract

The invention relates to an efficient mycoplasma hyopneumoniae culture medium and a preparation method thereof, and belongs to the technical field of veterinary biology. The efficient mycoplasma hyopneumoniae culture medium comprises an A liquid and a B liquid mainly consisting of MEM, yeast leaching powder, tryptone, glucose, inorganic salt and the like. The prepared culture medium of the invention has the main advantages of low serum content which is only 10%-15%, while the serum content in common culture medium is 20% even more. The culture medium prepared by the low serum relieves the pig allergic to the stress reaction, meanwhile gives consideration to the biosafety of animals. Besides the valence of the semi-finished bacterial solution prepared by the method is up to 109CCU/ml, which is much higher than the culture medium prepared by the common technology.

Description

technical field [0001] The invention belongs to the technical field of veterinary biology, and relates to a medium for cultivating mycoplasma hyopneumoniae with low serum and high efficiency and a preparation method thereof. Background technique [0002] Mycoplasma hyopneumoniae mainly causes chronic respiratory diseases in pigs, and is one of the most important diseases causing economic losses in the swine industry. Mycoplasma hyopneumoniae is widely distributed all over the world. Infection of pigs leads to poor growth and development, reduced growth rate and feed conversion rate, and it is easy to cause secondary virus or bacterial infection, resulting in increased mortality of pigs and heavy economic losses. The prevention of this disease should adopt comprehensive measures, providing an ideal environment for pigs is the basis, including ventilation, temperature and reasonable stocking density and feeding methods. Although drug treatment is effective, it consumes a lot o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/35
Inventor 不公告发明人
Owner 兆丰华生物科技(南京)有限公司
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