8733 results about "Culture mediums" patented technology

Systems for plant culture include a chamber featuring one or more walls enclosing a spatial volume internal to the chamber, where the one or more walls include a surface for supporting a plant within the enclosed spatial volume, a gas delivery apparatus with at least one gas source, a nutrient delivery apparatus with a reservoir, a sampling apparatus connected to a port formed in the one or more walls, and a controller configured so that during operation of the system, the controller activates the nutrient delivery apparatus to deliver an aqueous growth medium to the plant, and activates the gas delivery apparatus to deliver into the enclosed spatial volume a mixture of isotopically-substituted gases. Also provided are methods of use of the system for measuring nitrogen in a plant and for identifying microbes capable of providing fixed nitrogen to a plant.

The invention provides a virtual-real fusion method for digital twinning operation of a cell drug factory in combination with the Internet of Things, which comprises the following steps: constructing geometric models of a culture medium storage tank and a bioreactor through three-dimensional modeling software, mapping and associating historical process parameters of a culture medium supply system and temperature, pH value and dissolved oxygen concentration monitored in real time with the geometric models, and obtaining a virtual-real fusion model of the culture medium storage tank and the bioreactor; constructing a digital twinborn model of the reactor; analyzing historical production data through a machine learning algorithm, extracting a quantitative relationship among key parameters of a cell metabolism rate, a glucose concentration in a culture medium, a pH value and a reactor temperature, establishing a cell growth prediction model, and predicting a growth trend and a metabolism demand of cells in a target time period; a virtual-real mapping mechanism of a culture medium supply system is integrated with an intelligent manufacturing execution system, so that the culture medium supply process and the whole pharmaceutical production process are subjected to collaborative optimization control, and the working procedures of the culture medium production process are automatically dispatched according to a production plan and cell growth requirements.

An automatic material supplementing method based on a real-time dynamic measurement result, the method comprising: collecting a real-time dynamic measurement result, and calculating a change rate of each component within a unit time, wherein the change rate is a consumption rate or a generation rate; selecting a component having the highest change rate as a marker within a current time period; collecting historical measurement data, and calculating a cell growth rate in a logarithmic growth phase and a target product expression rate in a cultivation and harvesting stage; taking, as a set component concentration value within the current time period of the logarithmic growth phase, a concentration level of the marker when the cell growth rate in the logarithmic growth phase is the highest; taking, as a set component concentration value within the current time period of the cultivation and harvesting stage, a concentration level of the marker when the target product expression rate in the cultivation and harvesting stage is the highest; and calculating an addition dosage of the marker within the current time period on the basis of the change rate of the marker and / or the difference between a real-time measured concentration of the marker within the current time period and the set component concentration value within the current time period, and calculating an addition dosage of a culture medium on the basis of the addition dosage of the marker, so as to obtain a material supplementing strategy.

The present invention provides a serum-free culture medium for in vitro suspension culture of porcine muscle stem cells and its application, which can help porcine muscle stem cells expand and be suspended under serum-free and carrier-free conditions. Compared with traditional porcine muscle stem cell culture protocols and culture media, this serum-free suspension culture medium enables porcine muscle stem cells to be cultured continuously at high densities in both batch and fed-batch culture processes under serum-free and carrier-free suspension culture conditions, while maintaining high cell viability and achieving a peak cell density of 1×10 7 The beneficial effect of the present invention is to effectively solve the problem of serum-free and carrier-free efficient suspension expansion of cell cultured meat seed cells, and provide a technical and raw material basis for the industrial production of cell cultured meat.

The invention discloses an intelligent pretreatment workstation for microbiological detection, and relates to the technical field of microbiological detection. The module comprises a culture dish turntable, a transmission part, a homogeneous slapping part, a vortex oscillation part, a material rack storage part, a culture medium feeding part and the like; the culture dish turntable module comprises an upper dish station, a liquid injection station, an inoculation / coating station, a lower dish station and the like, as well as a transplanting electric module and a shaking-up module; the liquid injection unit can be used for injecting three culture solutions; the culture dish conveying module is responsible for conveying culture dishes to corresponding stations and is connected with an external feeding and receiving unit; the material rack storage module is used for storing pipettor tips, test tubes and homogenizing bags; the homogeneous slapping and vortex oscillation module is used for sample dilution and homogenization; the clamping robot is responsible for conveying the homogeneous bag and the test tube to the corresponding module and pulling out the plug of the test tube; the pipetting robot is used for sucking a sample and filling the sample into the culture dish. The steps from sample to culture can be completed at high speed and high efficiency, and the steps can be adjusted according to actual detection conditions through modular combination.

The invention provides a cell culture medium aided design system and method based on a large oracle model, and belongs to the technical field of artificial intelligence. A plurality of candidate culture medium formula schemes meeting user requirements are generated by obtaining culture medium design parameters and requirements and designing structured cues and inputting the designed structured cues into a large language model; a substance matched with the structured cue word is retrieved by utilizing a retrieval enhancement technology, a replacement substance is recommended for a user to refer, the user selects and purchases a required experimental material according to own requirements, a candidate culture medium formula scheme is fed back and evaluated in an actual experiment, and the system performs iterative optimization based on the feedback and evaluation, so that the accuracy of the experiment is improved. And a more appropriate candidate culture medium formula scheme is provided. According to the cell culture medium aided design system and method, intelligence, scientization and individuation of culture medium formula scheme design are achieved, experiment cost is reduced, repeatability and stability of cell culture experiments are improved at the same time, and therefore wide application of culture medium design in the field of biological manufacturing is promoted.

The invention provides a technical method for improving the anti-aging effect of eurotium cristatum tea compound fermentation liquor, and belongs to the technical field of microorganisms, the Latin name of eurotium cristatum is Eurotium cristatum, the eurotium cristatum is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 2024643. According to the method, the yeast seed solution is inoculated into the tea-sea buckthorn leaf culture solution fermented by the eurotium cristatum, so that the content of anti-aging active components such as procyanidine B2, quercitrin, cyanidin-3-glucoside, nicotinic acid, naringenin and rutin in the fermentation culture solution can be remarkably increased through co-fermentation, and the elastase inhibition rate can be remarkably increased; the anti-aging effect of a component system of the fermentation culture solution is enhanced. The prepared tea-hippophae rhamnoides leaf compound fermentation liquor is suitable for development of anti-aging cosmetics and has a wide market prospect.

The invention discloses aureobasidium pullulans, application thereof and a method for producing medium-molecular-weight pullulan, and belongs to the technical field of microorganisms. The aureobasidium pullulans disclosed by the invention is aureobasidium pullulans SYCY-018, the strain is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on December 26, 2022, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.40467. According to the aureobasidium pullulans SYCY-018, the aureobasidium pullulans SYCY-018 and the aureobasidium pullulans The aureobasidium pullulans SYCY-018 is inoculated into a fermentation medium for fermentation to produce the pullulan, the yield of the medium-molecular-weight pullulan reaches up to 150 g / L or above, meanwhile, good hereditary stability and high temperature resistance are achieved, the yield of the pullulan can still be higher than 130 g / L even at the high temperature of 40 DEG C, and the aureobasidium pullulans SYCY-018 is suitable for industrial production.

The invention discloses a saline-alkaline tolerant strain as well as a culture method and application thereof, and relates to the technical field of agricultural biology. Comprising the following steps: S1, screening and culturing strains; s11, carrying out salt tolerance detection on primary strains stored in a laboratory, then carrying out first screening, and screening out strains with OD600 values greater than 1.0 as candidate strains; s12, inoculating the candidate strains into a culture medium, carrying out secondary screening, and screening out strains with the OD600 value greater than 1.0 as saline-alkaline tolerant strains; and S13, inoculating the saline-alkaline tolerant strain into the EPS-producing strain fermentation medium. The application of the saline-alkaline tolerant strain provided by the invention can obviously improve the yield and quality of crops; experiments show that after the HB-02 strain is inoculated, the prevention and treatment effect on wheat root rot reaches 80.2%, the yield of wheat in saline-alkali soil is increased to 703.13 g / m < 2 > from 516.61 g / m < 2 >, the yield is increased by 36.10%, and good disease prevention and growth promotion effects are shown.

The invention discloses a genetically engineered algae capable of resisting aquatic pathogenic bacteria as well as a construction method and application thereof, and is characterized in that the genetically engineered algae is phaeodactylum tricornutum into which cell wall energizing plasmids pPha-T1-J48054-eGFP-Linker-Q4 or pPha-T1-J48054-eGFP-Linker-Q6 are introduced, and the preparation method of the genetically engineered algae comprises the following steps: taking genome DNA of the phaeodactylum tricornutum as a template; the method comprises the following steps: constructing a directional expression plasmid pPha-T1-J48054-eGFP by amplifying a Phatr3J48054 coding sequence, inserting Q4 and Q6 coding sequences into the downstream of the eGFP of a directional expression vector, constructing a cell wall energizing plasmid, electrically transforming the cell wall energizing plasmid into phaeodactylum tricornutum, coating an f / 2 plate culture medium with transformed phaeodactylum tricornutum cells for culturing, screening positive algae strains, and screening the positive algae strains. The feed additive has the advantage of inhibiting or killing aquatic pathogenic bacteria.

The invention provides a vascularized organ-like chip based on seal microstructure patterning and a preparation method thereof, the chip comprises a micro-channel layer, the upper surface of the micro-channel layer is provided with a gel perfusion channel, a central tissue chamber and culture medium flow channels at two sides which are connected, and tumor spheres or colon cells are inoculated in the central tissue chamber; the liquid storage pool layer is arranged on the upper surface of the micro-channel layer and corresponds to the inlet and outlet of the gel filling channel, the inlet and outlet of the central tissue chamber and the inlet and outlet of the culture medium runner, the liquid storage pool layer is provided with a plurality of liquid storage pools and a plurality of through holes, and the liquid storage pools contain culture solutions to provide nutrients for cultured tissues; and the seal module is arranged above the central tissue chamber, the seal module is provided with a tumor seal and a colon seal, and the tumor seal and the colon seal are respectively provided with a microstructure three-dimensional module and are respectively used for simulating the culture environment of tumor spheres or colon cells. According to the invention, in-vitro construction of the vascularized tumor model and the colon model is realized, and a guide is provided for construction of the vascularized organ-like model with specific organ three-dimensional microstructure characteristics.

The present application discloses a cell culture unit, device, application and culture method, wherein the cell culture unit includes a substrate, a culture chamber, a liquid storage chamber and a flow channel chamber are formed in the substrate, and the culture chamber and the liquid storage chamber have an opening at the top of the substrate, and the two ends of the flow channel chamber are connected to the culture chamber and the liquid storage chamber respectively, and the flow channel chamber includes a first port portion connected to the liquid storage chamber and a second port portion connected to the culture chamber, and the flow channel chamber is also formed with a constriction portion between the first port portion and the second port portion, and the opening area of ​​the constriction portion is smaller than the opening area of ​​the first port portion and the second port portion. The technical solution of the present application can avoid the impact on the biological sample caused by too fast flow rate, resulting in the cells in the liquid gel being dispersed by the culture medium, and increase the time for the culture medium to infiltrate the biological sample to fully infiltrate the biological sample, thereby being able to improve the simulation degree of the cell model finally obtained and improve the success rate of culture.

The invention belongs to the technical field of biological material engineering, and particularly relates to a fungal imitation leather material and a preparation method thereof. The method comprises the following steps: firstly, adopting wild fomes fomentarius subjected to 60Co irradiation mutagenesis as a production strain of a mycelium pad, and screening and optimizing components and content of a liquid culture medium to obtain a surface culture liquid culture medium suitable for the wild fomes fomentarius; secondly, by utilizing the strain and an optimized culture medium, the low-cost and large-scale production of the mycelium pad can be realized under the optimal culture condition; and the mycelium pad is subjected to various physical and chemical treatments such as plasticizing, cross-linking, hot-pressing compaction and the like, so that the mechanical strength of the mycelium pad is further improved, and the mechanical property requirements of leather products are met.

The invention belongs to the technical field of microbial fermentation and food engineering, and discloses a fermentation method for enhancing the production capacity of schizochytrium limacinum phospholipid DHA and application, schizochytrium limacinum is subjected to fermentation culture for 32-60 h, and the addition of an organic nitrogen source and phosphate is optimized in the fermentation culture process; the formula of a seed culture medium for culturing schizochytrium limacinum is not changed, and only the formula of a fermentation culture medium for schizochytrium limacinum is changed. The invention provides a fermentation strategy for enhancing the production capacity of the schizochytrium limacinum phospholipid DHA, and the schizochytrium limacinum can obtain the phospholipid DHA with higher content by optimizing an organic nitrogen source and phosphate in a schizochytrium limacinum fermentation culture medium. According to the obtained schizochytrium limacinum grease, the total DHA content is obviously increased compared with that of a traditional fermentation strategy, and the proportion of phospholipid DHA is also remarkably increased. The regulation and control method is applied to preparation of schizochytrium limacinum algae powder, the schizochytrium limacinum algae powder rich in phospholipid DHA is obtained, and application of the schizochytrium limacinum algae powder in the field of aquatic feed is facilitated.

The invention belongs to the technical field of mushroom culture, and discloses a mushroom culture shelf lifting mechanism for a mushroom house, the mushroom culture shelf lifting mechanism comprises a rotating mechanism, the rotating mechanism comprises a first gear and a screw rod, bevel gears are fixedly connected to the sides, close to each other, of the first gear and the screw rod, and the screw rod is in threaded sleeve connection with a threaded sleeve; through cooperation of structures such as an inclined plate and a sliding rod, a rotating disc can conveniently rotate on a placement plate, after a first gear is meshed with a tooth groove, the rotating disc can drive a culture pot to rotate by 180 degrees and then continue to ascend and descend through transmission, it is ensured that each mushroom can receive light evenly, and the mushroom quality is improved. The illumination receiving time of different surfaces of mushrooms is kept consistent, so that the uniform growth of hyphae is promoted, the problem of excessive growth or uneven growth is avoided, meanwhile, different parts of a culture medium can obtain better conditions in the aspects of light receiving, atmospheric circulation and the like through rotation, uniform distribution and absorption of nutrients are promoted, and the growth rate of the hyphae is increased. The overall nutrition efficiency of mushroom growth is improved.

The invention relates to the technical field of microbial culture, and discloses a cell culture device, which comprises: a plate body; the culture units are arranged on the plate body at intervals in the width direction of the plate body, and each culture unit comprises a plurality of base holes which are arranged at intervals in the length direction of the plate body and are used for culturing cells and fluid channels which are arranged between every two adjacent base holes; according to the flowing direction of the culture medium along the fluid channels, the two sections of fluid channels corresponding to the two upward sides of each base hole are called the upstream fluid channel and the downstream fluid channel of the base hole; a downstream hole wall is arranged at least between the fluid channel at the downstream of the base hole and the base hole, and a plurality of downstream gaps allowing the culture medium to pass through and limiting the cells to pass through are formed in the downstream hole wall in a cutting manner.

The invention discloses a method for high-precision measurement of the diameter of an inhibition zone of a culture medium. The method comprises the following steps: S1, image acquisition and pretreatment: acquiring a culture medium image and carrying out standardized pretreatment on the acquired image; s2, semantic segmentation of the inhibition zone: performing network training on the preprocessed petri dish image based on deep learning; and S3, accurate edge detection: extracting an accurate boundary of the inhibition zone based on a semantic segmentation result, and adopting a sub-pixel-level edge detection algorithm. And S4, scale identification and calibration: automatically detecting and positioning the measurement scale in the image by adopting an intelligent image identification algorithm, and integrating a calibration precision verification mechanism to realize real-time automatic calibration of measurement data. And S5, diameter calculation and result output: based on the results of S3 and S4, calculating the equivalent diameter of the inhibition zone by adopting a geometric fitting algorithm, and generating a standardized measurement report. According to the method, deep learning and image processing technologies are fused, high-precision automatic measurement of the diameter of the inhibition zone is realized, and the method has strong environmental adaptability.

The invention discloses burdock root fermentation liquor as well as a preparation method and application thereof in cosmetics, and belongs to the technical field of cosmetics. The preparation method provided by the invention comprises the following steps: inoculating a culture medium with pseudoalteromonas or schizosaccharomyces pombe for activation to obtain a seed solution; the seed solution is inoculated into a fermentation medium, burdock root powder is added, the adding concentration of the burdock root powder in the fermentation medium is 45-55 g / L, then fermentation is conducted to the end point, and after-treatment is conducted, the burdock root fermentation liquor is obtained. The burdock root fermentation liquor prepared by the preparation method disclosed by the invention has excellent anti-oxidation effect, anti-allergy soothing effect and tightening effect, also has certain healing promoting and repairing functions, is safe, non-toxic and small in irritation, and can be used for preparing anti-oxidation, anti-allergy and soothing cosmetic raw materials.

The invention provides a serum-free culture medium for in-vitro suspension culture of porcine muscle stem cells and application of the serum-free culture medium. The serum-free culture medium can help the porcine muscle stem cells to be subjected to suspension enlarged culture under serum-free and carrier-free conditions. Compared with a traditional porcine muscle stem cell culture scheme and culture medium, the serum-free suspension culture medium has the advantages that high-density continuous culture of porcine muscle stem cells can be realized in a batch culture process and a fed-batch culture process under the serum-free carrier-free suspension culture condition, high cell viability is maintained, and the survival rate of the porcine muscle stem cells is increased. The peak density of the cells reaches 1 * 10 < 7 > cells / mL or above, and the cell viability is maintained to be 90% or above. The method has the beneficial effects that the problem of serum-free carrier-free efficient suspension amplification of the seed cells of the cell culture meat is effectively solved, and a technical and raw material basis is provided for industrial production of the cell culture meat.

The invention relates to cordyceps sinensis fermentation filtrate as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) mixing cordyceps sinensis powder with an alcoholic solution, carrying out ultrasonic extraction, centrifuging to take supernate, and drying to obtain a cordyceps sinensis matrix extract; (2) mixing the cordyceps sinensis powder, the cordyceps sinensis matrix extract and a basic fermentation culture medium to obtain a main fermentation culture medium, and inoculating the main fermentation culture medium with a zymophyte solution for fermentation culture to obtain a fermentation solution; and centrifuging the fermentation liquor, filtering, and collecting the filtrate to obtain the cordyceps sinensis fermentation filtrate. By optimizing the fermentation process, the cordyceps sinensis powder and the cordyceps sinensis matrix extract obtained by ultrasonic extraction of the cordyceps sinensis powder are jointly fermented, so that the bioavailability of the cordyceps sinensis can be fully improved, the functional components in the cordyceps sinensis fermentation filtrate are enriched, and the health-care effect of the cordyceps sinensis is improved. The prepared cordyceps sinensis fermentation filtrate has an excellent anti-aging effect.

The invention discloses a pig chimeric embryo culture medium and application thereof in effectively improving the in-vitro chimeric ability of pig embryonic stem cells, and belongs to the technical field of bioengineering. According to the pig chimeric embryo culture medium disclosed by the invention, a neutral culture environment for promoting synchronous development of stem cells and embryos is created by accurately regulating and controlling growth factors, nutrient substances and other key components in the culture medium. On the basis, overexpression of anti-apoptosis factors, optimization of microinjection conditions and adoption of a laser-assisted incubation means are combined, so that the chimeric efficiency is further improved, integration of the stem cells and embryos is effectively supported, the chimeric efficiency is improved, and a reliable in-vitro tool is provided for evaluating the chimeric ability and pluripotency of the pig stem cells.

The invention provides a method for preparing recombinant human albumin with low O-glycosylation level, comprising taking at least one measure to improve the growth rate of the strain and reduce the O-glycosylation level of the protein during the fermentation process, and specifically comprising S1, seed liquid preparation; S2, BSM culture medium is put into a fermentation tank, the pH is adjusted to 5.0-5.8, sterilization is carried out, dissolved oxygen is corrected after cooling, temperature and ventilation volume are set, sterilized PTM1 solution is added to the culture medium, and the pH of the culture medium is adjusted to 5.0-5.8 again; S3, shaking flask seeds are connected to the fermentation tank, dissolved oxygen is corrected, and rotation speed is set; S4, when the dissolved oxygen increases suddenly, a glycerol feeding stage is entered, and glycerol feeding is added at a uniform speed; S5, glycerol feeding and methanol feeding are added simultaneously, pH is adjusted to 6.0-6.5, and the temperature is lowered; S6, methanol feeding is added; S7, after the fermentation is completed, the tank is released, the fermentation product is centrifuged, and the supernatant is collected; the method of the invention can reduce protein O-glycosylation, and the expressed recombinant protein contains a very low level of glycosylation modification.

The invention discloses convenient-to-adjust shaking-up equipment for cell culture, and relates to the technical field of cell culture. The shaking-up device comprises a shaking-up plate which is obliquely arranged in a shell, a plurality of holes are formed in the surface of the shaking-up plate, a limiting assembly is fixedly connected to the inner side face of the shaking-up plate, a driving assembly is fixedly connected to the bottom of the inner side face of the shell, and the side, close to an end cover, of the driving assembly is in spline clamping connection with the surface of the limiting assembly. The clamping assembly is fixedly connected with the inner wall of the hole of the shaking plate, the shaking plate is obliquely arranged, the culture bottle not only generates horizontal circular motion in the rotating process, but also generates up-and-down displacement along with the inclined surface of the shaking plate, and the culture bottle is subjected to a complete ascending and descending process in the process of revolving around the driving assembly for a circle; a three-dimensional composite oscillation effect is formed, so that a culture medium and a culture bottle are promoted to generate complex relative movement, the contact between cells and nutrient substances can be more efficiently promoted, and the gas exchange efficiency is enhanced.

The invention discloses a chromogenic culture medium for detecting gram-negative bacteria in bovine mastitis and a test plate thereof, the culture medium comprises a nutritional ingredient mixture, a composite inhibitor, a composite chromogenic enzyme substrate and cold water gel, the compound chromogenic enzyme substrate is red tetrazole, 5-bromo-6-chloro-3-indole-beta-D-galactoside, and 5-bromo-4-chloro-3-indole-beta-D-glucopyranoside, and the compound chromogenic enzyme substrate is a compound chromogenic enzyme substrate, and the compound chromogenic enzyme substrate is 5-bromo-6-chloro-3-indole-beta-D-glucopyranoside. The chromogenic culture medium and the test board prepared from the chromogenic culture medium can effectively inhibit the growth of non-gram negative bacteria, have the characteristics of strong specificity, high sensitivity, short detection period, easy observation and low detection cost, and have huge development potential and wide application prospects in microbiological detection.

The invention discloses a closed mixotrophic microalgae culture method and a culture system thereof, and relates to the field of microalgae culture, comprising the following steps: step one, microalgae culture, pouring culture solution into a culture tank, adding microalgae to be cultured into the culture solution, and then fixing a sealing plate to the tank mouth of the culture tank by bolts to carry out closed culture; step two, gas supply and nutrient solution addition, sending carbon dioxide from a pipeline into an air distribution pipe through an air pump in an air supply component, forming bubbles from a nozzle and transporting them into the culture solution, and part of the bubbles that rise are gathered by a baffle to extend their residence time in a water body; step three, illumination, during the day, a first electric telescopic rod drives an arc-shaped telescopic plate to move downward to expose the internal transparent culture tank, and sunlight provides a light source to propagate the microalgae. The application can extend the residence time of carbon dioxide in the water body, improve the utilization rate of carbon dioxide, and increase the yield of microalgae.

The invention relates to the field of piggery tail end odor pollution treatment, and discloses a piggery tail end odor purification filter element preparation method and application, the preparation method comprises the following steps: loading a photocatalyst on the surface of a filter element body to obtain a carrier, respectively inoculating 3-5 kinds of antagonism-free deodorization microorganisms into corresponding LB liquid culture mediums for activation and propagation, and carrying out purification on the LB liquid culture mediums to obtain the piggery tail end odor purification filter element. The preparation method comprises the following steps: preparing a bacterial solution, mixing the bacterial solution to obtain a corresponding bacterial solution, compounding all the bacterial solutions in proportion to obtain a compound bacterial solution, infiltrating a carrier in the compound bacterial solution, and carrying out biofilm formation at room temperature to obtain the filter element. After the photocatalyst on the surface is illuminated, some refractory pollutants can be decomposed into smaller intermediate products which are easier to utilize by microorganisms by utilizing light energy, a more suitable substrate is provided for the microorganisms, and the microorganisms can further thoroughly decompose the intermediate products into harmless substances such as carbon dioxide and water, so that the degradation effect is improved. Therefore, the overall degradation efficiency and degree of pollutants are improved.

Described in several exemplary embodiments herein are serum replacement compositions, cell culture media containing said serum replacement compositions, and uses thereof.

Optimization of the formula of a culture medium for industrially producing a Bacillus velezensis TCS001 fermentation broth and culture process conditions therefor. By using the culture medium and the culture process conditions, not only can the fermentation production cost be reduced, but also the effective viable bacterium count and the spore count in the Bacillus velezensis TCS001 fermentation broth can be improved, so that the fermentation broth can be used for industrial production and market promotion and application. Provided is a lipopeptide-compound-containing mixture separated from a Bacillus velezensis TCS001 fermentation broth. By further optimizing the formula of the culture medium for the Bacillus velezensis TCS001 fermentation broth and the culture fermentation process conditions, the output and yield of lipopeptide compounds in the fermentation broth are successfully improved, providing an efficient and cost-effective solution for the control of plant diseases.

The present invention relates to a preparation method of a pine mushroom mycelium fermentation product, and its products and applications, including: inoculating pine mushroom liquid seeds into a culture medium for culturing, solid-liquid separation, obtaining pine mushroom mycelium and fermentation filtrate; subjecting the fermentation filtrate to alcohol precipitation treatment to obtain pine mushroom mycelium crude polysaccharide; inoculating fermentation bacteria seed liquid into a fermentation medium added with pine mushroom mycelium and pine mushroom mycelium crude polysaccharide for culturing to obtain a fermentation liquid; centrifuging the fermentation liquid, filtering the supernatant and sterilizing to obtain the pine mushroom mycelium fermentation product. The present invention develops a new fermentation product with pine mushroom mycelium as a fermentation substrate, the fermentation product has excellent soothing and antioxidant effects, and compared with the method of fermenting a single pine mushroom mycelium or a single pine mushroom mycelium crude polysaccharide, the fermentation product of the present invention has better soothing and antioxidant effects.

The invention discloses lactobacillus plantarum capable of relieving enteritis and toxicity of polystyrene nano-plastics, and the preservation number of the lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.31420. The lactobacillus plantarum can be used for relieving enteritis and toxicity of polystyrene nano-plastics. The strain is obtained by separating and purifying baby feces, and can grow on an MRS culture medium, and the bacterial colony is round, off-white, smooth and opaque. Experiments show that the lactobacillus plantarum can effectively adsorb PS-NPs, promote the PS-NPs to be discharged along with excrement, reduce accumulation of the PS-NPs in organs, and significantly reduce toxicity and inflammatory response of organs such as liver and kidney of mice. In addition, the strain can repair the intestinal barrier function, regulate liver metabolism and relieve enteritis symptoms. The invention further provides a probiotic preparation containing the strain, and the probiotic preparation can be prepared into granules, capsules, tablets or oral liquid and the like and is used for preventing or treating intestinal injury and inflammatory diseases caused by PS-NPs. The strain is safe and efficient, and has a wide application prospect.