6721 results about "Culture mediums" patented technology

An automatic material supplementing method based on a real-time dynamic measurement result, the method comprising: collecting a real-time dynamic measurement result, and calculating a change rate of each component within a unit time, wherein the change rate is a consumption rate or a generation rate; selecting a component having the highest change rate as a marker within a current time period; collecting historical measurement data, and calculating a cell growth rate in a logarithmic growth phase and a target product expression rate in a cultivation and harvesting stage; taking, as a set component concentration value within the current time period of the logarithmic growth phase, a concentration level of the marker when the cell growth rate in the logarithmic growth phase is the highest; taking, as a set component concentration value within the current time period of the cultivation and harvesting stage, a concentration level of the marker when the target product expression rate in the cultivation and harvesting stage is the highest; and calculating an addition dosage of the marker within the current time period on the basis of the change rate of the marker and / or the difference between a real-time measured concentration of the marker within the current time period and the set component concentration value within the current time period, and calculating an addition dosage of a culture medium on the basis of the addition dosage of the marker, so as to obtain a material supplementing strategy.

The present invention provides a serum-free culture medium for in vitro suspension culture of porcine muscle stem cells and its application, which can help porcine muscle stem cells expand and be suspended under serum-free and carrier-free conditions. Compared with traditional porcine muscle stem cell culture protocols and culture media, this serum-free suspension culture medium enables porcine muscle stem cells to be cultured continuously at high densities in both batch and fed-batch culture processes under serum-free and carrier-free suspension culture conditions, while maintaining high cell viability and achieving a peak cell density of 1×10 7 The beneficial effect of the present invention is to effectively solve the problem of serum-free and carrier-free efficient suspension expansion of cell cultured meat seed cells, and provide a technical and raw material basis for the industrial production of cell cultured meat.

The invention provides a cell culture medium aided design system and method based on a large oracle model, and belongs to the technical field of artificial intelligence. A plurality of candidate culture medium formula schemes meeting user requirements are generated by obtaining culture medium design parameters and requirements and designing structured cues and inputting the designed structured cues into a large language model; a substance matched with the structured cue word is retrieved by utilizing a retrieval enhancement technology, a replacement substance is recommended for a user to refer, the user selects and purchases a required experimental material according to own requirements, a candidate culture medium formula scheme is fed back and evaluated in an actual experiment, and the system performs iterative optimization based on the feedback and evaluation, so that the accuracy of the experiment is improved. And a more appropriate candidate culture medium formula scheme is provided. According to the cell culture medium aided design system and method, intelligence, scientization and individuation of culture medium formula scheme design are achieved, experiment cost is reduced, repeatability and stability of cell culture experiments are improved at the same time, and therefore wide application of culture medium design in the field of biological manufacturing is promoted.

The invention discloses aureobasidium pullulans, application thereof and a method for producing medium-molecular-weight pullulan, and belongs to the technical field of microorganisms. The aureobasidium pullulans disclosed by the invention is aureobasidium pullulans SYCY-018, the strain is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on December 26, 2022, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.40467. According to the aureobasidium pullulans SYCY-018, the aureobasidium pullulans SYCY-018 and the aureobasidium pullulans The aureobasidium pullulans SYCY-018 is inoculated into a fermentation medium for fermentation to produce the pullulan, the yield of the medium-molecular-weight pullulan reaches up to 150 g / L or above, meanwhile, good hereditary stability and high temperature resistance are achieved, the yield of the pullulan can still be higher than 130 g / L even at the high temperature of 40 DEG C, and the aureobasidium pullulans SYCY-018 is suitable for industrial production.

The invention belongs to the technical field of microbial fermentation and food engineering, and discloses a fermentation method for enhancing the production capacity of schizochytrium limacinum phospholipid DHA and application, schizochytrium limacinum is subjected to fermentation culture for 32-60 h, and the addition of an organic nitrogen source and phosphate is optimized in the fermentation culture process; the formula of a seed culture medium for culturing schizochytrium limacinum is not changed, and only the formula of a fermentation culture medium for schizochytrium limacinum is changed. The invention provides a fermentation strategy for enhancing the production capacity of the schizochytrium limacinum phospholipid DHA, and the schizochytrium limacinum can obtain the phospholipid DHA with higher content by optimizing an organic nitrogen source and phosphate in a schizochytrium limacinum fermentation culture medium. According to the obtained schizochytrium limacinum grease, the total DHA content is obviously increased compared with that of a traditional fermentation strategy, and the proportion of phospholipid DHA is also remarkably increased. The regulation and control method is applied to preparation of schizochytrium limacinum algae powder, the schizochytrium limacinum algae powder rich in phospholipid DHA is obtained, and application of the schizochytrium limacinum algae powder in the field of aquatic feed is facilitated.

The invention discloses a method for high-precision measurement of the diameter of an inhibition zone of a culture medium. The method comprises the following steps: S1, image acquisition and pretreatment: acquiring a culture medium image and carrying out standardized pretreatment on the acquired image; s2, semantic segmentation of the inhibition zone: performing network training on the preprocessed petri dish image based on deep learning; and S3, accurate edge detection: extracting an accurate boundary of the inhibition zone based on a semantic segmentation result, and adopting a sub-pixel-level edge detection algorithm. And S4, scale identification and calibration: automatically detecting and positioning the measurement scale in the image by adopting an intelligent image identification algorithm, and integrating a calibration precision verification mechanism to realize real-time automatic calibration of measurement data. And S5, diameter calculation and result output: based on the results of S3 and S4, calculating the equivalent diameter of the inhibition zone by adopting a geometric fitting algorithm, and generating a standardized measurement report. According to the method, deep learning and image processing technologies are fused, high-precision automatic measurement of the diameter of the inhibition zone is realized, and the method has strong environmental adaptability.

The invention provides a serum-free culture medium for in-vitro suspension culture of porcine muscle stem cells and application of the serum-free culture medium. The serum-free culture medium can help the porcine muscle stem cells to be subjected to suspension enlarged culture under serum-free and carrier-free conditions. Compared with a traditional porcine muscle stem cell culture scheme and culture medium, the serum-free suspension culture medium has the advantages that high-density continuous culture of porcine muscle stem cells can be realized in a batch culture process and a fed-batch culture process under the serum-free carrier-free suspension culture condition, high cell viability is maintained, and the survival rate of the porcine muscle stem cells is increased. The peak density of the cells reaches 1 * 10 < 7 > cells / mL or above, and the cell viability is maintained to be 90% or above. The method has the beneficial effects that the problem of serum-free carrier-free efficient suspension amplification of the seed cells of the cell culture meat is effectively solved, and a technical and raw material basis is provided for industrial production of the cell culture meat.

The invention relates to cordyceps sinensis fermentation filtrate as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) mixing cordyceps sinensis powder with an alcoholic solution, carrying out ultrasonic extraction, centrifuging to take supernate, and drying to obtain a cordyceps sinensis matrix extract; (2) mixing the cordyceps sinensis powder, the cordyceps sinensis matrix extract and a basic fermentation culture medium to obtain a main fermentation culture medium, and inoculating the main fermentation culture medium with a zymophyte solution for fermentation culture to obtain a fermentation solution; and centrifuging the fermentation liquor, filtering, and collecting the filtrate to obtain the cordyceps sinensis fermentation filtrate. By optimizing the fermentation process, the cordyceps sinensis powder and the cordyceps sinensis matrix extract obtained by ultrasonic extraction of the cordyceps sinensis powder are jointly fermented, so that the bioavailability of the cordyceps sinensis can be fully improved, the functional components in the cordyceps sinensis fermentation filtrate are enriched, and the health-care effect of the cordyceps sinensis is improved. The prepared cordyceps sinensis fermentation filtrate has an excellent anti-aging effect.

The invention discloses convenient-to-adjust shaking-up equipment for cell culture, and relates to the technical field of cell culture. The shaking-up device comprises a shaking-up plate which is obliquely arranged in a shell, a plurality of holes are formed in the surface of the shaking-up plate, a limiting assembly is fixedly connected to the inner side face of the shaking-up plate, a driving assembly is fixedly connected to the bottom of the inner side face of the shell, and the side, close to an end cover, of the driving assembly is in spline clamping connection with the surface of the limiting assembly. The clamping assembly is fixedly connected with the inner wall of the hole of the shaking plate, the shaking plate is obliquely arranged, the culture bottle not only generates horizontal circular motion in the rotating process, but also generates up-and-down displacement along with the inclined surface of the shaking plate, and the culture bottle is subjected to a complete ascending and descending process in the process of revolving around the driving assembly for a circle; a three-dimensional composite oscillation effect is formed, so that a culture medium and a culture bottle are promoted to generate complex relative movement, the contact between cells and nutrient substances can be more efficiently promoted, and the gas exchange efficiency is enhanced.

The invention relates to the field of piggery tail end odor pollution treatment, and discloses a piggery tail end odor purification filter element preparation method and application, the preparation method comprises the following steps: loading a photocatalyst on the surface of a filter element body to obtain a carrier, respectively inoculating 3-5 kinds of antagonism-free deodorization microorganisms into corresponding LB liquid culture mediums for activation and propagation, and carrying out purification on the LB liquid culture mediums to obtain the piggery tail end odor purification filter element. The preparation method comprises the following steps: preparing a bacterial solution, mixing the bacterial solution to obtain a corresponding bacterial solution, compounding all the bacterial solutions in proportion to obtain a compound bacterial solution, infiltrating a carrier in the compound bacterial solution, and carrying out biofilm formation at room temperature to obtain the filter element. After the photocatalyst on the surface is illuminated, some refractory pollutants can be decomposed into smaller intermediate products which are easier to utilize by microorganisms by utilizing light energy, a more suitable substrate is provided for the microorganisms, and the microorganisms can further thoroughly decompose the intermediate products into harmless substances such as carbon dioxide and water, so that the degradation effect is improved. Therefore, the overall degradation efficiency and degree of pollutants are improved.

The invention discloses lactobacillus plantarum capable of relieving enteritis and toxicity of polystyrene nano-plastics, and the preservation number of the lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.31420. The lactobacillus plantarum can be used for relieving enteritis and toxicity of polystyrene nano-plastics. The strain is obtained by separating and purifying baby feces, and can grow on an MRS culture medium, and the bacterial colony is round, off-white, smooth and opaque. Experiments show that the lactobacillus plantarum can effectively adsorb PS-NPs, promote the PS-NPs to be discharged along with excrement, reduce accumulation of the PS-NPs in organs, and significantly reduce toxicity and inflammatory response of organs such as liver and kidney of mice. In addition, the strain can repair the intestinal barrier function, regulate liver metabolism and relieve enteritis symptoms. The invention further provides a probiotic preparation containing the strain, and the probiotic preparation can be prepared into granules, capsules, tablets or oral liquid and the like and is used for preventing or treating intestinal injury and inflammatory diseases caused by PS-NPs. The strain is safe and efficient, and has a wide application prospect.

The invention relates to the technical field of microorganisms, in particular to a culture medium and a method for culturing Ackermania muciniphila for activating Amuc-1260 by using lambda-carrageenan as a carbon source, according to the culture method, the lambda-carrageenan is used for replacing a traditional carbon source, thallus growth and proliferation can be remarkably promoted, the effect is optimal under the condition of 2.00 g / L, and the culture cost is low. Compared with the conventional carbon source (glucose), the carbon source is improved by 92.91%. Further research finds that the lambda-carrageenan can induce the Akk bacteria to up-regulate the expression of glycoside hydrolase such as Amuc-1260 and enhance the activity of pathways such as glycosaminoglycan degradation, amino acid metabolism and insulin signals, so that the metabolic adaptability and energy utilization efficiency of the bacteria are improved. Meanwhile, the Akk bacteria secrete more functional short-chain fatty acids such as acetic acid, propionic acid and the like under the condition, and have the effects of regulating intestinal health and maintaining metabolic homeostasis. The invention provides a new theoretical basis and technical support for large-scale culture of Akk bacteria and development of a metabiotic product.

The invention provides a whole-process intelligent management system for stem cell culture medium development, and belongs to the technical field of intelligent management. According to the method, artificial intelligence, big data analysis and knowledge graph technologies are fused, and the whole process from demand analysis, formula intelligent design and virtual simulation, small-scale culture verification and iterative optimization, pilot scale-up process optimization to quality compliance and tracing is covered. All the modules are coordinated through the central intelligent decision engine, the research and development efficiency of the culture medium can be remarkably improved, the development cost can be greatly reduced, and batch consistency and quality stability of the culture medium are ensured by reducing trial and error and optimizing in real time. Meanwhile, a full-chain electronic tracing function is provided, the requirements of laws and regulations for compliance are comprehensively met, and the problems that traditional research and development are low in efficiency, high in cost, unstable in batch and the like are effectively solved.

The invention relates to the technical field of microbial genetic engineering, in particular to a method for improving cordycepin synthesized by saccharomyces cerevisiae and saccharomyces cerevisiae engineering bacteria. According to the invention, codon-optimized 2 '-carbonyl-3'-deoxyadenosine reductase gene and 3 '-adenosine monophosphate phosphohydrolase gene from cordyceps militaris are expressed by using plasmids, and then the plasmids are transformed into host cells to obtain the saccharomyces cerevisiae engineering bacteria. And inoculating a seed solution obtained by seed culture of the activated saccharomyces cerevisiae engineering bacteria into a fermentation culture medium containing a metabolic effector, and carrying out fermentation culture to synthesize cordycepin. Metabolic effectors (Cu < 2 + >, Fe < 2 + >, Mg < 2 + >, Zn < 2 + >, citric acid, cysteine, aspartic acid, glycine, VB1 and tea polyphenol) are added into a fermentation culture medium strain, and the variety and concentration of the metabolic effectors are optimized, so that the yield and synthesis efficiency of cordycepin are remarkably improved. By using the combination of the strain obtained by the invention and the optimal effector, the yield of cordycepin in a fermentation tank reaches 2.9 g / L.

The invention discloses a microgravity culture dish and a using method, and relates to the technical field of biological culture devices.The microgravity culture dish comprises a culture dish body and a culture dish cover, the culture dish body comprises a culture bottom shell and a sealing cover, a breathable separation ring is arranged in the middle of the culture bottom shell, and a culture area is arranged on the inner side of the breathable separation ring; a humidifying area is arranged on the outer side of the breathable separation ring, and a combined filter membrane is arranged on the inner side of the breathable separation ring; a culture medium channel is arranged on the side face of the culture bottom shell, a sealing plug is arranged on the culture medium channel, the culture bottom shell is provided with a gas exchange channel, and a water absorption body is arranged in the humidification area. Meanwhile, the invention discloses a using method based on the microgravity culture dish, a liquid dynamic exchange function and a gas exchange function are achieved at the same time in a suspension culture mode, cell development morphology observation and judgment can be effectively improved, and evaporation of a culture medium is inhibited in a humidification area. A partitioning mechanism is arranged, and culture of different regions is carried out according to the size of an actual sample.

The invention belongs to the technical field of microorganisms, and particularly relates to a method for producing dark isolated endophytic fungus cladosporium chlamydospore through liquid fermentation. The culture medium and culture conditions suitable for growth of the Cladosporium kwangsiensis HX2 are found out through experiments such as single factor and response surface optimization, the yield of the chlamydospore produced by the Cladosporium kwangsiensis is greatly improved through optimization, the production cost of the chlamydospore is effectively reduced, and the shelf life of the fungicide is prolonged; through measurement and calculation, compared with a traditional PDB culture medium, the yield of chlamydospore is increased by 139.8%, the cost is reduced by 47%, and the shelf life is prolonged by about 8 months. The culture medium is simple in preparation, stable in fermentation process and suitable for large-scale production, the prepared chlamydospore preparation is high in stress resistance and long in shelf life, and a new effective thought is provided for industrialization of bottle cladosporium strains in the later period.

The invention relates to the technical field of microorganisms and particularly discloses bacillus velezensis for producing tetramethylpyrazine and application of the bacillus velezensis, the preservation number of the strain is CCTCC NO: M20232058, and the bacillus velezensis is obtained by separating and purifying from high-temperature Daqu of Maotai-flavor liquor in the liquor industry of Jin-brand Maotai Town in Guizhou. The bacillus velezensis disclosed by the invention can produce 1875.4 mg / L of tetramethylpyrazine by performing shake flask fermentation on a liquid culture medium for 7 days, and the tetramethylpyrazine can reach 4810.8 mg / L by optimizing a culture medium formula and culture conditions. The bacillus velezensis is applied to small-scale brewing of Maotai-flavor liquor, the content of tetramethylpyrazine in the Maotai-flavor liquor reaches 34.5 + / -1.4 mg / L and is increased by 325.93% compared with a contrast, and the bacillus velezensis can be used for increasing the content of tetramethylpyrazine in the Maotai-flavor liquor.

The invention relates to a phellinus igniarius mycelium fermentation product and a preparation method and application thereof, and the preparation method comprises the following steps: inoculating a phellinus igniarius mycelium seed solution into a sterile fermentation culture medium for culturing, carrying out solid-liquid separation, filtering a clear liquid through a membrane and sterilizing to obtain the phellinus igniarius mycelium fermentation product, the fermentation medium comprises the following components: a beet extract, a deep eutectic solvent and water. The beet extract and the deep-eutectic solvent are creatively adopted as culture medium components to prepare the phellinus igniarius mycelium fermentation product, the beet extract and the deep-eutectic solvent jointly provide nutrients needed by phellinus igniarius fermentation, meanwhile, the deep-eutectic solvent is used for regulating metabolism, and compared with a product obtained through fermentation of a traditional culture medium, the phellinus igniarius mycelium fermentation product has the advantages that the fermentation efficiency is high; the content of active ingredients in a fermentation product obtained by adopting the culture medium with the formula is remarkably increased, and the fermentation product is good in uniformity and high in stability, is a clear liquid in appearance, has no abnormal odor and has slight phellinus igniarius characteristic odor.

The invention relates to the field of fermentation processes, in particular to a method for preparing 1, 4-butanediol through multi-strain synergistic fermentation, which comprises the following steps: inoculating a first strain and a second strain into a culture medium containing glucose and / or xylose for synergistic fermentation, producing ornithine by the first strain by taking glucose and / or xylose as a substrate, and converting ornithine into 1, 4-butanediol by the second strain. The invention discloses 1, 4-butanediol. Glucose is converted into ornithine through the first strain, ornithine is converted into 1, 4-butanediol through the second strain, the first strain and the second strain are subjected to synergistic fermentation, preparation of 1, 4-butanediol through microbial synergistic fermentation is achieved, and higher metabolic capacity and higher production efficiency are achieved. The mixed sugar of xylose and glucose prepared from biomass raw materials is used as a substrate for fermentation, so that the method has the advantages that the raw materials are easy to obtain, renewable and low in cost, and the production cost can be reduced.

The invention relates to a method for biosynthesizing zero-valent nano-selenium by utilizing pantoea, the method comprises the following steps of: adding selenite into a strain culture medium, culturing pantoea, and separating and purifying from a synthetic product to obtain the zero-valent nano-selenium, the strain name of the pantoea is XZ-1, the classification name is pantoea Pantoeasp., the preservation number is CGMCC No.32330, the collection number is CGMCC No.32330, the collection number is CGMCC No.32330, the collection number is CGMCC No.32330, the collection number is CGMCC No.32330, the collection number is CGMCC No.32330, and the collection number is CGMCC No.32330. The preservation date is October 24, 2024, the strain XZ-1 is applied to synthesize zero-valent nano-selenium, biological nano-selenium is obtained from the synthesized product after multiple times of separation and purification, and the purified biological nano-selenium is mixed with a protective agent and a dispersing agent according to a certain proportion to prepare a nano-selenium fertilizer raw material, so that the problems that the nano-selenium is agglomerated and is easy to oxidize and lose efficacy are solved; the bioavailability of selenium is obviously improved, and the selenium element loss is reduced. The prepared nano-selenium can be used for original ingredients of fertilizers and feeds.

The invention relates to an organ-like core-shell microsphere as well as a preparation method and application thereof. The preparation method comprises the steps that 1, raw materials are prepared, specifically, shell raw materials and inner core raw materials are prepared, the shell raw materials comprise a photoinitiator and methacrylated hyaluronic acid (HAMA), and the inner core raw materials comprise organoid precursor cell suspension and matrigel; 2) molding: extruding the dispersion phase of the shell raw material wrapping the core raw material to a continuous phase by using a micro-fluidic chip and a high-precision injection pump, cutting the dispersion phase into liquid drops by the continuous phase, and performing illumination curing on the liquid drops to form core-shell microspheres; and (3) incubating, namely culturing the core-shell microspheres in a culture medium, so that the organoid precursor cells in the inner core are developed into organoids. According to the preparation method, rapid forming and curing of the organ-like precursor cells carried by the matrigel are achieved, so that the time of the organ-like precursor cells staying in the oil phase is shortened, the cells can obtain oxygen and nutrient substances, and the cell activity is improved.

The invention discloses a mother strain culture medium for boletus xylophilus as well as a preparation method and application of the mother strain culture medium, and belongs to the technical field of microbial culture. The preservation number of the strain JSJ-Bx1 of the boletus sinensis mother strain is CGMCC (China General Microbiological Culture Collection Center) No.42007, and the strain JSJ-Bx1 is preserved in the China General Microbiological Culture Collection Center; the culture medium is prepared from 10-30 g of a carbon source, 2-6 g of a nitrogen source, 1-3 g of inorganic salt, 0.1-0.3 g of a growth factor suitable for the culture medium, 20 g of agar and 1 L of water, and the pH of the culture medium is 4-6. By the adoption of the culture medium, hyphae of the boletus sinensis mother strain grow fast, grow vigorously and are dense, the edges of bacterial colonies are neat, hypha degeneration phenomena such as pigment and water spitting are avoided, and the mother strain culture growth cycle is greatly shortened.

The invention discloses a kitchen waste oil-based sophorolipid high-yield candida utilis strain and an application thereof. Belongs to the technical field of directional domestication breeding. According to the method, wild candida bombicola is used as an original strain, and is sequentially domesticated in a culture medium containing kitchen waste oil with the concentration gradient of 2%, 4%, 6%, 8% and 10% to obtain the S.bombicola x10. The invention further discloses an application of the domesticated strain in fermentation production of sophorolipid, after the strain is subjected to shake flask fermentation, the sophorolipid yield reaches 60.19 g / L and is improved by 16.22% compared with that of a wild strain, and the acid type sophorolipid yield is improved by 32.11%. When the concentration of the lactone sophorolipid generated by fermentation is 1.25 mg / mL, the growth of the bacillus subtilis is completely inhibited. The invention provides an excellent production strain for industrial production of sophorolipid, and has good theoretical research significance and practical application value.

A method for enhancing hydrogen uptake by cells of an acetogenic bacterium in a culture is provided. The method comprises incubating the cells in a liquid medium supplemented with an external gas mixture, wherein the external gas mixture comprises H2 and CO2; overexpressing an uptake hydrogenase and an associated electron transfer protein in the cells; increasing uptake of the H2 by the cells; and enhancing uptake of the CO2 by the cells. The culture may be a coculture with additional cells of a non-acetogenic bacterium, which produces natively H2 from catabolism of an organic molecule.

The invention provides a culture medium formula capable of effectively improving vibrio inhibition capability of bacillus. The culture medium formula comprises 10-20 g of corn starch, 2-10 g of yeast powder, 2-10 g of malt extract, 5-20 g of glycerin, 0.5-1 g of L-aspartic acid, 1 g of dipotassium phosphate, 0.3 g of monopotassium phosphate, 0.5 g of magnesium sulfate, 1 g of calcium carbonate and 1000 mL of distilled water. When the culture medium is used for culturing bacillus capable of naturally inhibiting vibrio, the obtained culture has a better vibrio inhibiting effect.

The invention discloses a fermentation method of recombinant bacillus subtilis for expressing vibrio parahaemolyticus outer membrane protein, which comprises the following steps: inoculating a recombinant bacillus subtilis strain into a liquid culture medium for activation and amplification to obtain a seed solution; transferring the seed solution into a fermentation tank according to the inoculum size of 5%-10%, controlling the temperature to be 35-37 DEG C and the pH to be 6.8-7.2, introducing sterile air and stirring, and monitoring the thallus concentration OD600 value in real time; when the OD600 value reaches 35-38, 10% of an inducer is added for pre-induction; when the OD600 value reaches 40-45, the remaining 90% of the inducer is added for main induction, and the real-time dissolved oxygen value is collected; when the real-time dissolved oxygen value is continuously higher than a first preset threshold value, the flow acceleration rate of the inducer is increased, the fermentation temperature is increased at the same time or step by step, and the increase amplitude of the dissolved oxygen value is in positive correlation with the flow acceleration rate and the temperature increase amplitude; when the OD600 value is stabilized in a preset interval, it is judged that fermentation is completed, and the recombinant bacillus subtilis thalli are obtained. The yield and quality of the vibrio parahaemolyticus outer membrane protein can be effectively improved.

Disclosed are methods of treating infertility or increasing fertility of a female subject by administering a therapeutic MSC secretome product made by a method comprising culturing bone marrow-derived MSCs under conditions that include oxygen tension below 5% and a culture media with a pH below 7.

The invention discloses a biological hydrogen production method for coupling a coal seam microbial community and beet pulp waste. The biological hydrogen production method comprises the following steps: step 1, collecting coal seam microorganisms; step 2, sample pretreatment; 3, preparing a culture solution; step 4, flora enrichment culture; and 5, constructing a biological hydrogen production system to obtain hydrogen-rich gas. Microorganisms adopted in the method are original microorganisms in underground in-situ coal seam or ground coal-bed gas well drainage and mining water, sugar industry waste beet pulp is adopted as a hydrogen production substrate, and aiming at the bottlenecks of high strain cost, limited substrate applicability and the like in an existing biological hydrogen production technology, the coal seam microorganisms and sugar industry waste residues are coupled, so that the yield of hydrogen is increased. A low-cost hydrogen production system is constructed, so that the economic cost in the microbial hydrogen production process is reduced, and the beet pulp has the advantages of being safe, easy to obtain, low in cost and high in gas production rate, has the advantages of being easy and convenient to operate, economical, efficient, environmentally friendly and the like, and has a good industrial application prospect.

The invention discloses a method for inducing nematode-trapping fungus predatory organ formation, and belongs to the field of applied microorganisms. The method comprises the following specific steps: culturing activated nematode-trapping fungi until spores are generated; washing off the cultured spores, and diluting to obtain a spore suspension with the concentration of 30000-60000 spores / mL; coating the spore suspension into an agar culture medium paved with glass paper, uniformly coating the spore suspension, culturing for 28-36 hours at the temperature of 25-28 DEG C, adding a mixed solution of a predatory organ inducer and water, and continuously culturing for 36-60 hours at the temperature of 25-28 DEG C, so that the nematode-trapping fungi can generate a large number of predatory organs; the predatory organ inducer is one of oleic acid, linoleic acid or pregnenolone. It is found for the first time that a large number of predatory organs can be generated when pregnenolone, oleic acid or linoleic acid is used for inducing nematode-trapping fungi.

The invention belongs to the technical field of preparation of probiotic products, and relates to a lactic acid bacteria microcapsule as well as a preparation method and application thereof. The method comprises the following steps: uniformly mixing sodium alginate, modified montmorillonite and sterile water, cooling, adding a prepared lactic acid bacteria seed solution, and fully and uniformly mixing to form an emulsion; spraying the emulsion into a calcium chloride solution by adopting an extrusion method, stirring and curing to obtain microcapsules; and culturing the prepared microcapsule in a lactic acid bacteria liquid culture medium to obtain the lactic acid bacteria microcapsule after the culture is finished. The lactobacillus is effectively protected by adopting an extrusion-method single coating and post-coating culture process, so that the stability of the lactobacillus to a humid and hot environment and the gastrointestinal stress resistance are improved, and meanwhile, the release performance of the lactobacillus in the gastrointestinal tract is ensured. The problem that an existing microcapsule coating technology cannot achieve balance between improvement of the hygrothermal stability and gastrointestinal stress resistance of the probiotics and guarantee of good release performance of the probiotics in the gastrointestinal tract is solved.