19279 results about "Culture mediums" patented technology

Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. The medium may be conditioned by stromal cells, parenchymal cells, mesenchymal stem cells, liver reserve cells, neural stem cells, pancreatic stem cells and/or embryonic stem cells. Additionally, the cells may be genetically modified. A three-dimensional tissue construct is preferred. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, formulated with a salve or ointment for topical applications, or, for example, made into or added to surgical glue to accelerate healing of sutures following invasive procedures. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.

A significantly increased amount of a monoclonal antibody is obtained from the culture medium of recombinant hybridoma prepared by introducing genes encoding a protein identical to the immunoglobulin heavy chain polypeptide of the specific monoclonal antibody into an immortalized B cell (hybridoma) producing the monoclonal antibody.

Lipo-chito oligosaccharides (LCOs) are produced by culturing rhizobacteria cells in or on a culture medium comprising at least one of: jasmonic acid or a derivative thereof; linoleic acid or a derivative thereof; or linolenic acid or a derivative thereof. Preferably, the rhizobacteria cells are Bradyrhizobium japonicum cells having the identifying characteristics of B. japonicum strain USDA 3. Preferably, the derivative of jasmonic acid is an ester thereof, preferably methyl jasmonate. Also provided are methods for improving LCO production at low temperatures, particularly temperatures below 25° C.

The present invention provides adipose-derived stem cells and lattices. In one aspect, the present invention provides a lipo-derived stem cell substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. The invention also provides a method of isolating stem cells from adipose tissues. The cells can be employed, alone or within biologically-compatible compositions, to generate differentiated tissues and structures, both in vivo and in vitro. Additionally, the cells can be expanded and cultured to produce hormones and to provide conditioned culture media for supporting the growth and expansion of other cell populations. In another aspect, the present invention provides a lipo-derived lattice substantially devoid of cells, which includes extracellular matrix material from adipose tissue. The lattice can be used as a substrate to facilitate the growth and differentiation of cells, whether in vivo or in vitro, into anlagen or even mature tissues or structures.

Expression systems are disclosed for the direct expression of peptide products into the culture media where genetically engineered host cells are grown. High yield was achieved with novel vectors, a special selection of hosts, and/or fermentation processes which include careful control of cell growth rate, and use of an inducer during growth phase. Special vectors are provided which include control regions having multiple promoters linked operably with coding regions encoding a signal peptide upstream from a coding region encoding the peptide of interest. Multiple transcription cassettes are also used to increase yield. The production of amidated peptides using the expression systems is also disclosed.

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides methods for separating, characterizing and/or identifying microorganisms in situ within a single system.

A photobioreactor includes a cultivation zone configured to contain a liquid culture medium and facilitate growth of a microalgae biomass, a plurality of parallel edge-lit light transmitting devices mounted within the cultivation zone, and a collection zone oriented in relation to the cultivation zone such that at least a portion of the liquid culture medium and microalgae from the cultivation zone may be periodically harvested. Methods for illuminating algae, for dissolving materials into an algae medium, for extracting oil from algae, and for producing biodiesel from algal oil are also provided.

While culture medium and systems have been described that permit the culture and proliferation of human embryonic stem cells in feeder free and animal product free conditions, these conditions will not readily support cloning of an embryonic stem cell culture meaning, at least here, the initiation of a sub-culture using one or a very few originating cells. It has been found here that a class of small molecules that are inhibitors of kinase enzymes will increase the efficiency of cloning of stem cell cultures sufficiently to make such cloning practical in the defined medium and in other media as well.

A media supplement for culturing anaerobic bacteria is provided which comprises a filtrate of effluent from a chemostat vessel in which a target bacterial ecosystem has been cultured. Methods of using the supplement for culturing or isolating anaerobic microbial strains or communities, particularly anaerobic bacteria from the human gut, are also provided.

The invention relates to the technical field of edible fungus cultivation, in particular to a Chinese medicinal herb culture medium, a preparation method thereof and a cultivation method of edible fungi. The culture medium is made by a mixture and water, wherein the mixture is manufactured by the following components by weight percent: 65 percent to 70 percent of Chinese medicinal herbs, 30 percent to 35 percent of cottonseed hulls and 2 percent to 3 percent of calcium superphosphate; and the total weight ratio of the water and the mixture is 1 : 1. In the preparation method, the Chinese medicinal herbs are used as main culture medium to cultivate the edible fungi with the efficacies of invigorating kidney and qi, nourishing a liver, reinforcing yin and tranquilization, and the like, thereby the health-care function of the edible fungi to a human body is enhanced, and the method can be used for cultivating golden mushrooms, pleurotus eryngii, bailing mushrooms, tea mushrooms, pocket-sized mushrooms, coprinus comatus, oyster mushrooms, phoenix mushrooms, ganoderma lucidum, auricularia polytricha, auricularia auricula, dictyophora indusiata, hypsizigus marmoreus, stropharia rugoso-annulata, nameko, pleurotus abalonus, fistulina hepatica, brazilian mushrooms, mushrooms, straw mushrooms and tremella. The invention also provides the preparation method of the Chinese medicinal herb culture medium and the method for cultivating the edible fungi by using the Chinese herb culture medium.

The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for interrogation of the separated microorganism sample by mass spectrometry to produce a mass spectrum of the microorganism and characterizing and/or identifying the microorganism in the sample using the mass spectrum obtained.

There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical.

The invention discloses a culture medium for culturing mesenchymal stem cells. The culture medium comprises improved minimum medium IMDM, cell growth factor combination, insulin, trace elements, lipid substances, vitamin substances, hormone substances, protein molecules, amino acid substances and other compounds. The culture medium provided by the invention does not contain serum or plasma (comprising animal or human serum or plasma), thus, when the system is used for culturing the mesenchymal stem cells, zoonosis caused by the animal serum or plasma in the cell therapy process can be avoided, and the risk of human immunity reaction which is possibly caused in the cell therapy process can be avoided. The culture medium has specific chemical constituent, thus the possibility of different cultured mesenchymal stem cell properties caused by the difference of culture systems can be avoided. The culture medium is not only suitable for culturing the mesenchymal stem cells of human marrow and umbilical cord resources but also suitable for culturing the mesenchymal stem cells of other tissues or other animals, and has the advantage of wide application range.

The invention relates to a bioreactor comprising a chamber for containing cells or tissue cultures within a culture medium. The bioreactor also comprises a detector capable of detecting a change in one or more metabolites associated with growth of the cell or tissue cultures within the chamber and a chamber drive capable of rotating the chamber at a first speed about a first axis and a second speed about a second axis, the second axis being disposed at an angle relative to the first axis. In use, the magnitude of the first speed and the second speed are independently variable of each other.

The invention discloses a novel edible fungus culture medium and a preparation method thereof. The novel edible fungus culture medium comprises 70-85% of main material, 15-20% of auxiliary material, 3-5% of additive material and 0.1-0.8% of absorbent resin, wherein the main material comprises sawdust, straws, corncobs, and cottonseed hull; the auxiliary material comprises bran, rice bran and maize meal; and the additive material comprises land plaster, slaked lime, calcium superphosphate, white sugar and urea. The preparation method disclosed by the invention comprises the following steps of: reasonably selecting raw materials in the ratio of nutrient ingredients according to the nutritional requirements of different kinds of edible fungi to prepare the culture medium, and adding the absorbent resin synthesized by the biological materials. The permeability and the water retaining capacity of the culture medium are greatly enhanced, and the accumulation of the various nutrient elements and the slow releasing capacity to the nutrient elements are enhanced; meanwhile, the properties of the cellulose and the protein of the absorbent resin provide the nutrition in the aspect of carbon source or nitrogen source so as to ensure the sufficient supplement of the required raw materials of the fungi; and by adopting the novel edible fungus culture medium disclosed by the invention, the output of a single bag is enhanced by 25%-35%, and the fungus output time is advanced by 4-6 days.

The present invention provides a small (business card-sized) disposable mesofluidic or microfluidic plastic cartridge containing several straight microchannels potentially filled with culture media, solubilizing reagents (e.g., detergents) nitrocellulose paper strip, gel or other matrix materials and lyophilized paramagnetic microbeads or microparticles coated with antibodies, nucleic acid aptamers, oligonucleotides, or other types of proteins or other receptors for capture, concentration and ultrasenstive detection of target analytes in environmental, food, animal, or clinical body fluid samples.

The cultivation of terrestrial plants in brackish water or seawater is carried out with this invention. A light-weight, floating growth medium package (FGMP) or, alternatively, a sheet of suitable material is used to support the growth of terrestrial plants floating on water bodies of various salinity, including 100% seawater in marine environments. The FGMP units can be linked together and confined in a floating, rigid or flexible framework to form a floating seawater cultivation platform (FSCP). Using the method, plants were able to grow and thrive on the FSCP floating on 100% seawater in a sustainable manner. Halophytic akulikuli (Sesuvium portulacastrum L.) can regenerate its shoot and root in seawater. Thus, the discovery will enable us to practice marine agriculture, or agriculture on the sea. The FSCP can be used for wide range of purposes, from environmental protection to landscaping to crop production.

The invention discloses a production method for a tea vinegar drink, and relates to the technical field of processing of drinks. The tea vinegar drink is mainly prepared from tea, momordica grosvenori, white sugar, mountain spring water and acetic acid bacterium. The production method comprises the following steps: after the momordica grosvenori is smashed, the tea, the momordica grosvenori, the white sugar, the mountain spring water and the acetic acid bacterium are respectively weighed, and the weighed raw materials are placed in a pot and boiled to obtain an extract liquid; an acetic acid bacterium strain accounting for 2-3 percent of the total weight of the raw materials is -inoculated, covered and fermented, the product temperature is controlled to be 16-28 DEG C, and the fermentation lasts 10-25 days; acid adjustment is performed on a fermented and obtained feed liquid, the total acid content of the feed liquid is controlled within a range of 0.3-1 g/100ml, and the soluble solid is controlled within a range of 3-5 percent; the tea vinegar drink is obtained via vacuum and sterile filling after the liquid is sterilized. The invention discloses the tea vinegar drink integrating multiple functions of nutrition and health care, vegetal syrup is used as an acetic acid bacteria culture medium for fermentation, the produced tea vinegar drink contains various spicy substances and nutrient ingredients of the tea, the momordica grosvenori and acetate acid, and is good in flavor and high in quality.

Well-defined, xeno-free culture media which comprise a TGF-beta isoform or the chimera formed between IL6 and the soluble IL6 receptor (IL6RIL6), which are capable of maintaining stem cells, and particularly, human embryonic stem cells, in an undifferentiated state are provided. Also provided are cell cultures comprising the culture media and the stem cells and methods of expanding and deriving embryonic stem cells in such well-defined, xeno-free culture media. In addition, the present invention provides methods of differentiating ESCs or EBs formed therefrom for the generation of lineage specific cells.

The present invention claims and discloses a novel apparatus and method for efficiently cultivating cells with minimal mortality in order to harvest a maximum amount of cellular products generated by the cultivated cells. More particularly, the present invention teaches a method and a device for plating cells and causing maximum adherence of cells of interest. Furthermore, the present invention also teaches a growth substrate means that is capable of providing the largest surface area for cell adhesion and functions as an oxygenator, a depth filter and a static mixer to maximize the production of cellular products by intermittently and periodically provide sufficient oxygen and nutrients to the cells without causing cell death. The device of the present invention is economical and can be disposable thus eliminating complications caused by sterlization and is capable of periodically and intermittently provide oxygen and nutrients to cells, through controlling the amount of culture medium that comes into contact with the growth substrate means.

Culture media and methods for expanding and differentiating populations of stem cells and for obtaining organoids. Expanded cell populations and organoids obtainable by methods of the invention and their use in drug screening, toxicity assays and regenerative medicine.

Described are methods for isolating/purifying and expanding animal stem cells and stem-cell-like cells. Isolation methods include conditions comprising preferentially digesting non-stem cells and non-stem-cell-like cells in a population and preferentially adhering stem cells and stem-cell-like cells in a population. Expansion methods include culturing such cells under conditions comprising modulation of TGF-β signaling, inhibition of cell signaling mediated by p38 MAP kinase using small molecular weight inhibitors, expansion of the cells on human amniotic epithelial cells as feeder layers, control of cell seeding density, control of levels of Ca²⁺ in the culture media, rapid adhesion on a substrate or by a combination of such conditions. More particularly, what is disclosed relates to methods and systems for expanding animal cells in ex vivo cell cultures, while preventing cellular differentiation, and selectively enriching stem cells. The embodiments also disclose a culture system for ex vivo expansion of limbal epithelial cells or mesenchymal cells, as well as surgical grafts made there from.

The invention discloses a mixed plant culture medium and a preparation method thereof. The culture medium comprises peat and an agricultural and forest residue compositing fermentation medium, wherein the volume proportion of peat to agricultural and forest residue compositing fermentation medium is (30-50):(50-70). The mixed culture medium prepared by the method in the invention has the advantages of balanced nutrition, high nutrition component contents and stable physical property. The mixed culture medium prepared by the method in the invention is reasonable in nutrition structure and convenient for absorption of foliage plants and meets the growth requirement of the foliage plants, thereby improving the culture survival rate and viewing effect of the foliage plants.

A method for cultivating Antrodia Camphorata is provided. First, Antrodia Camphorata is pre-incubated on a sloped surface culture medium. A portion of the pre-incubated Antrodia Camphorata is transferred to a liquid culture medium for incubation. Then, Antrodia Camphorata incubated in the liquid culture medium is moved to a solid culture medium for incubation. The cultivated Antrodia Camphorata has similar efficacies for inhibiting cancel cell growth, reducing the occurrence of atherosclerosis or recovering the damaged liver functions, as the wild Antrodia Camphorata.

PCT No. PCT/GB97/00669 Sec. 371 Date Sep. 11, 1998 Sec. 102(e) Date Sep. 11, 1998 PCT Filed Mar. 12, 1997 PCT Pub. No. WO97/33973 PCT Pub. Date Sep. 18, 1997A process of culturing a microorganism in a culture medium in which process the addition of feed medium is controlled by using the production of a by-product as a measure of the culture conditions, characterized in that the by-product is an electrically charged metabolite produced by the microorganism, and in that the production of the metabolite is monitored by measuring the conductance of the culture medium. The metabolite may be acetate and the microorganism may be yeast which is genetically engineered to produce a desired polypeptide.

The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition wherein indeterminate structures are formed in a liquid medium, the structures are uniformly dispersed in the solution and substantially retain the cells and/or tissues without substantially increasing the viscosity of the solution, thus affording an effect of preventing sedimentation thereof, and the like.

Disclosed herein are yeasts, which, when cultured, can produce relatively high concentrations of lactic acid. Also disclosed herein are culture media that result in relatively lower levels of by-product impurities when lactic acid-producing yeast are cultured in them.

The present invention provides media for inactivating pathogens found within protein-containing biological fluids. The media of the present invention preserve the structural integrity and biological activity of labile proteins while simultaneously exhibiting potent disinfectant activity. The media of the present invention comprise iodinated chromatographic media, particularly ion exchange media. The invention further provides methods for disinfecting biological fluids.

The invention relates to a method for preparing a bacillus subtilis lipopeptid biosurfactant, which is characterized in that the adopted bacterial strain is efficient lipopeptid producing strain bacillus subtilis BIT09S1,BIT09S2 and BIT09A2, and the lipopeptid biosurfactant is prepared by taking cheap molasses, pulse flour, konjac gum finemeal, guar gum and gum breaking liquid of modified guar gum as carbon sources for the culture medium; adding other nitrogen sources, phosphorus sources, inorganic salt components and nutrient elements into the culture medium; and carrying out fermentation, separation and purification. The raw materials used for producing lipopeptid by the method are easily obtained and can be enormously purchased. The crude extract of the obtained lipopeptid biosurfactant Surfactin can be used in various drilling and production processes such as oil-gas field fracturing, acidizing, de-plugging, controlling and water shut-off, oily water treatment, environment renovation and the like, and the biosurfactant has wide prospect.