501 results about "Coding region" patented technology

The invention provides a UTR (Untranslated Region) sequence for improving mRNA (Messenger Ribonucleic Acid) stability and translation efficiency and application of the UTR sequence, and particularly provides an mRNA molecule which comprises a coding region for coding polypeptide or protein and a fragment thereof, a 5 'UTR sequence positioned at the upstream of the coding region and / or a 3' UTR sequence positioned at the downstream of the coding region, the 5 'UTR sequence is a nucleotide sequence as shown in SEQ ID NO: 1, and the 3' UTR sequence is a nucleotide sequence as shown in SEQ ID NO: 2; and / or, the 5 'UTR sequence is a nucleotide sequence as shown in SEQ ID NO: 3, and the 3' UTR sequence is a nucleotide sequence as shown in SEQ ID NO: 4. According to the present invention, the mRNA stability and the translation efficiency are improved through the new and optimized UTR sequence, and the UTR sequence can be used as the element for enhancing the RNA expression efficiency in the nucleic acid treatment drug or the mRNA vaccine so as to provide more and better choices for the mRNA therapy.

The invention discloses an mRNA non-coding region sequence optimization method and system and electronic equipment, and the mRNA non-coding region sequence optimization method comprises the steps: constructing an initial candidate library according to a target protein; inputting the initial candidate library into a pre-trained mRNA sequence optimization model to obtain a prediction data set; performing multi-dimensional scoring and sequence optimization on the prediction data set to obtain a sequence recommendation group; performing biological verification on the sequence recommendation group to obtain an optimized mRNA sequence; wherein the prediction data set comprises a sequence ID, a sequence content, a prediction TE score and a confidence interval. According to the method, the translation efficiency of the mRNA sequence can be efficiently and accurately predicted, the candidate sequence with high expression potential is screened out, meanwhile, the consumption of computing resources is reduced, and the overall design cost is reduced.

The invention discloses application of an upstream non-coding region uORF of an ABA2 gene in regulation and control of crop agronomic traits, in particular to application in improvement of pre-harvest sprouting resistance of rice and cultivation of pre-harvest sprouting resistant rice strains, and belongs to the technical field of gene engineering. The invention finds that uORF in the upstream non-coding region of the crop ABA2 gene can inhibit the translation level of downstream ABA2 protein on the basis of not influencing the transcriptional level, so that the uORF can participate in regulation and control of crop agronomic traits in which ABA participates in regulation and control, such as improvement of pre-harvest sprouting resistance of rice. Therefore, the upstream non-coding region uORF of the crop ABA2 gene can be modified through a hybridization or gene editing technology, so that the purpose of regulating and controlling crop agronomic traits in which ABA participates in regulation and control is achieved, for example, site-directed mutagenesis is performed on an initiation codon of the upstream non-coding region uORF of the rice ABA2 gene, and the pre-harvest sprouting resistance of rice is improved.

The present invention belongs to the technical field of biological prevention and treatment for viral diseases. Disclosed in the present invention are a tomato disease-resistant gene mutant, and the use thereof in the prevention and treatment of ToBRFV. It is found in the present invention that mutating the nucleotide at position 1927 of the coding region sequence of tomato Tm-22 gene from G to A, or mutating the amino acid at position 643 of the LRR domain of a protein that is encoded by tomato Tm-22 gene from glycine to arginine can remarkably reduce the accumulation level of ToBRFV capsid protein (CP). The gene (named Tm-22-Mut5) can serve as a novel ToBRFV resistant gene, and also retains the resistance to TMV, ToMV and ToMMV. The Tm-22-Mut5 and a pre-screened Tm-22-Mut3-1 mutant (tyrosine at position 767 of the LRR domain thereof is mutated to phenylalanine) undergo combinatorial mutagenesis to obtain a mutant Tm-22-Mut6. Analysis shows that the Tm-22-Mut6 can remarkably reduce the accumulation level of the ToBRFV capsid protein. Compared with the pFGCTm-22-Mut3-1 mutant obtained by screening in the previous research and the newly obtained mutant Tm-22-Mut5, the Tm-22-Mut6 has further improved resistance to ToBRFV.

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA1 gene, an expression vector and application of the CeNF-YA1 gene in vegetable fat regulation and control. The invention provides the CeNF-YA1 gene for the first time, the nucleotide sequence of the CeNF-YA1 gene is as shown in SEQ ID NO.1, the full length of a coding region of the CeNF-YA1 gene is 792 bp, a series of carriers containing the CeNF-YA1 gene, such as subcellular localization, yeast hybridization and plant overexpression, are constructed for the first time, and meanwhile, the CeNF-YA1 protein is proved to have a transcription activation function, is localized in a cell nucleus and conforms to the basic characteristics of transcription factors. It is found for the first time that overexpression of the CeNF-YA1 gene can significantly improve the grease content of arabidopsis seeds and leaves; therefore, the technical scheme provided by the invention can be used for improving the oil content of the plant seeds and the nutritional tissues, and shows the application prospect in improving the oil content of the seeds and the nutritional tissues.

The disclosure includes methods and apparatuses that includes processing circuitry that derives a chroma prediction mode of a chroma block in a chroma coding region based on luma coding information of a luma block associated with a position in a reconstructed luma area that is collocated with the chroma block. The chroma coding region is partitioned separately from a collocated luma coding region including the reconstructed luma area. The luma coding information indicates that the luma block is coded with a first coding mode using a first luma BV (an IBC mode or an IntraTMP mode) and a second coding mode. The processing circuitry derives a chroma BV of the chroma block based on a luma BV of the luma block and reconstructs the chroma block based on the chroma prediction mode including the first coding mode and the second coding mode.

The invention relates to a high-throughput cis-acting element screening carrier and a screening method. Specifically, the invention provides a plasmid vector system containing bar codes, each bar code in the system is in one-to-one correspondence with a candidate cis-acting element, and the activation multiple of the candidate cis-acting element can be obtained by measuring the abundance of the bar codes; in order to eliminate the influence of a bar code on the vector on a detection result, an exogenous intron which can be cut off during transcription is inserted into a coding region in the vector and is used for distinguishing vector DNA and RNA obtained by transcription. The screening method of the biological cis-acting element has high efficiency, wide applicability and high throughput, and has outstanding application value in biological research and breeding.

The invention discloses an application of a multidrug-resistant efflux transporter gene OsMRET1 in regulation and control of chalkiness traits of rice. A nucleotide sequence of a coding region of the gene is shown as SEQ ID No.1, and a coded amino acid sequence is shown as SEQ ID No.2. The invention further discloses an application of the multidrug-resistant efflux transporter gene OsMRET1 in regulation and control of chalkiness traits of rice. It is found for the first time that the gene OsMRET1 can regulate and control formation of chalkiness, an OsMRET1 gene knockout mutant is created through a gene editing technology, an OsMRET1 gene overexpression strain is obtained through a transgenic technology, it is verified that the OsMRET1 gene is a new gene for regulating and controlling rice chalkiness, and overexpression of the gene can reduce rice chalkiness and improve rice chalkiness. And a new gene resource and a technical route are provided for rice quality improvement.

The invention provides application of a rice grain chalkiness regulation gene Chalk13, and belongs to the technical field of biology. According to the application, the rice grain chalkiness regulation and control gene Chalk13 is used for regulating and controlling formation of rice grain chalkiness, the nucleotide sequence of the rice grain chalkiness regulation and control gene Chalk13 is shown as SEQ ID NO: 1 in a sequence table, and the nucleotide sequence of a coding region of the rice grain chalkiness regulation and control gene Chalk13 is shown as SEQ ID NO: 2 in the sequence table. The invention provides an application of a rice grain chalkiness regulation and control gene Chalk13. The rice grain chalkiness regulation and control gene Chalk13 is used for regulating and controlling the chalkiness degree and the chalkiness rate of rice grains.

This invention relates to the field of genetic engineering technology, and provides a ZmPHYLL gene, the full-length nucleotide sequence of which is shown in SEQ ID NO: 1, and the coding region nucleotide sequence of which is shown in SEQ ID NO: 2. This invention also provides the application of the above-mentioned ZmPHYLL gene in inhibiting the germination of *Russula multifiliis* spores and improving the resistance of maize plants to southern maize rust. Simultaneously, this invention provides a method for improving the resistance of maize plants to southern maize rust. In this study, a ZmPHYLL gene was screened, and the expressed protein of this gene can effectively inhibit the germination of *Russula multifiliis* spores. Therefore, by regulating the overexpression of this gene, the resistance of maize to southern maize rust can be improved. This invention provides new gene resources for the creation of breeding materials and provides important scientific basis for the breeding of maize varieties highly resistant to southern maize rust.

The invention relates to guide polynucleotides and methods for targeting and editing a portion of the 5' UTR-encoding region of genes encoding VGSC alpha subunits (NaV) to abrogate or create an upstream open reading frame (uORF). The invention relates to guide polynucleotides and methods for targeting and editing a portion of the 5' splice acceptor site (SA) of an exon of a gene encoding a voltage-gated sodium channel (VGSC) alpha subunit (NaV). The invention also relates to use of the guide polynucleotides and methods for treating genetic disorders, in particular Dravet syndrome.

The invention belongs to the field of gene engineering, and relates to a plant genome directional editing tool based on transposon-coded nuclease. The invention aims to solve the technical problem that the application range of a plant genome editing tool based on CRISPR-Cas9 and Cas12a is limited to a great extent due to the fact that CRISPR-Cas9 and Cas12a are large in protein size and difficult to deliver. The invention provides transposon nuclease suitable for plant genome editing. The transposon nuclease is IsDge10, IsAam1 or enIscB subjected to codon preference optimization; and a gene editing system containing the three transposon nuclease is constructed. The gene editing system can be suitable for editing a coding region, a non-coding region and the like; the method can be widely applied to monocotyledonous plants, dicotyledonous plants, gymnosperm and the like.

The invention relates to an SNP molecular marker related to litchi fruit weight and application thereof, and belongs to the technical field of molecular biology. The SNP molecular marker related to the litchi fruit weight is based on a seedless litchi reference genome, the SNP molecular marker is located in a litchi SL05G0138630 gene coding region, and is located at the 27564818th basic group of a litchi chromosome 5, and the basic group is A or G. The application finds that a non-synonymous SNP site in a coding region of the litchi SL05G0138630 gene is closely associated with the litchi fruit weight phenotype, the variety with the weight of the litchi fruit can be efficiently identified by detecting the polymorphic site, the invention further discloses an identification method for the molecular level of the variety with the weight of the litchi fruit, and the molecular level of the variety with the weight of the litchi fruit can be identified by an SNP-based typing method. And cheap and accurate gene SNP typing can be achieved, so that the purpose of efficiently and accurately identifying the weight variety of the litchi fruit is achieved.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for germplasm identification and breeding of armeniaca mume, and belongs to the technical field of fruit tree molecular marker-assisted breeding. On the basis of re-sequencing and selective clearance analysis of wild plum and armeniaca mume populations, a key gene remarkably related to the armeniaca mume cyanin deletion character is identified, and an SNP variation with a codon terminated in advance exists in a gene coding region; a specific primer is further designed according to the functional SNP variation, a target fragment corresponding to a plum genome is amplified through PCR, and the base type of an SNP site is detected through first-generation sequencing, so that whether the detected plant is the armeniaca mume germplasm or not can be quickly and accurately judged. The SNP molecular marker can be used for early screening of armeniaca mume germplasm, has important application value, and provides an efficient and reliable technical means for molecular marker-assisted breeding.

The invention discloses a conservative non-coding element identification method and system based on multi-species genome alignment, and the method comprises the following steps: establishing an index database based on reference genome sequences of multiple species, completing whole genome alignment, and further processing to obtain a high-credibility chain alignment result; the multi-species chain type comparison results are integrated into multi-sequence comparison data in a unified format; on this basis, a neutral evolution model is constructed based on quadruple degenerate sites, and candidate conservative regions are predicted through conservative scoring; in combination with genome annotation information, a length threshold is set, and a coding region and a UTR region are rejected, so that a high-confidence non-coding conservative element is obtained; and finally, displaying a cross-species conservative distribution diagram of the CNE by utilizing a visual tool. According to the method, the CNE with a potential regulation function can be accurately, efficiently and automatically identified, and technical support is provided for regulation system analysis, functional gene mining and molecular breeding of various organisms.

The invention discloses application of SNP sites in identifying or screening watermelon fruit shapes, and belongs to the technical field of molecular biology. The SNP site is located at the 727th site of a Cla97C03G066390 gene coding region on a chromosome 3 of a watermelon Watermelon (97103) v2 genome, and the SNP site has G / A polymorphism and is applied to identification or screening of watermelon fruit shapes. The KASP molecular marker primer group for detecting the SNP site comprises specific reverse primers as shown in SEQ ID NO.2 and SEQ ID NO.3, and a universal forward primer as shown in SEQ ID NO.4. The invention also discloses a kit for detecting the KASP molecular marker primer group for detecting the SNP site. The SNP locus and the developed KASP molecular marker primer group can quickly perform genetic typing of watermelon fruit shapes, are accurate and efficient in detection and convenient and stable in amplification, and can be used for molecular marker-assisted selection and watermelon germplasm resource improvement.

The invention discloses an mRNA sequence scoring method and related equipment. The method comprises the following steps: acquiring an mRNA sequence to be scored; extracting a key base sequence from the mRNA sequence, wherein the key base sequence comprises an initiation codon context and a termination codon context; inputting the key base sequence into a target scoring model, and processing to obtain a score of the mRNA sequence in the target index; wherein the target scoring model comprises a multi-modal RNA sequence coding module and a large language model, and the target scoring model is obtained based on unsupervised pre-training of transcription sample data and supervised fine tuning of a score label data set corresponding to the target index. According to the method, a non-coding region and a coding region key region (start / stop codon context) are integrated as input, and a large language model trained based on mass data is used for processing, so that various complex factors such as sequence context information and secondary structures can be comprehensively considered, and the method can be widely applied to the technical field of gene sequence analysis.

The invention relates to the technical field of plant genetic engineering, in particular to application of an algae NySAT2 gene in regulation and control of alfalfa crude protein and detergent fiber. According to the invention, a sequence after codon optimization of an NySAT2 gene coding region is obtained by using gene synthesis and codon optimization technologies, an algae NySAT2 gene overexpression vector is constructed, a target gene is introduced into an alfalfa genome by using an agrobacterium rhizogenes mediated genetic transformation method, and a transgenic plant is obtained by using a genetic transformation system. Molecular identification and phenotypic analysis show that on the premise of keeping normal growth vigor (plant height and biomass have no significant difference from those of a wild type), the crude protein content of a medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased compared with that of the wild type medicago sativa plant, and meanwhile, the contents of acid detergent fibers and neutral detergent fibers are remarkably reduced, so that the content of the crude protein in the medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased. The forage quality and the feeding value of the medicago sativa are effectively improved.

The invention relates to a modified RNA with a synthetic cap structure and a modified coding region encoding for DNAse1 and / or DNAse1L3. The invention further relates to a saRNA and / or a circular RNA, and to a vector encoding the modified RNA. The invention also relates to a pharmaceutical composition for the use of treating a disease associated with the formation of extracellular DNA, including extracellular traps released from leukocytes, such as NETs.

The application discloses a method for quantitatively regulating gene translation and application thereof. The method comprises the following steps: sequencing translation efficiency of Kozak sequences and variants thereof before a start codon of a target gene or before an upstream open reading frame of a non-coding region of the target gene, and performing gene editing on the Kozak sequences according to a sequencing result, so as to realize in-situ manipulation of the Kozak sequences and quantitatively regulate gene translation, and the Kozak sequences and the variants thereof are 3-6 bp in length respectively. The application establishes a highly efficient, flexible and widely applicable gene expression regulation method. By using a precise gene editing tool to customize the Kozak sequences of the target gene in-situ, the expression level of the target gene can be quantitatively controlled at the gene translation stage.

This invention relates to the field of plant molecular breeding technology, specifically to a KASP molecular marker PH5-KASP based on the SNP site of the maize Zm00001eb259660 gene and its application. It provides a KASP molecular marker targeting the SNP site at 226029364 bp on maize chromosome 5, which is a functional missense mutation c.485C>T in the coding region of the Zm00001eb259660 gene. The marker consists of two allele-specific forward primers carrying different fluorescent tags and one universal reverse primer, stably distinguishing between C:C and T:T homozygous genotypes, where C:C corresponds to higher plant height and T:T corresponds to lower plant height. This invention offers high marker genotyping accuracy, simple and low-cost detection, and is suitable for large-scale breeding sample detection. It can be used for early identification of maize plant height traits, germplasm screening, and assisted breeding, significantly improving the efficiency of ideal maize plant architecture improvement.

The application discloses application of a rice disease resistance related gene Os04g55720 in bacterial blight breeding and relates to the technical field of modern biotechnology and genetic engineering technology.The rice disease resistance related gene Os04g55720 has a nucleotide sequence as shown in SEQ ID NO.1, is composed of 2821 nucleotides, has a coding region sequence as shown in SEQ ID NO.2, and has a sequence of 2148 nucleotides; the gene encodes a protein with 613 amino acids, and has an amino acid sequence as shown in SEQ ID NO.3.The application proves that overexpression of Os04g55720 enhances the resistance of rice to bacterial blight by means of molecular biology and biochemical technology, and shows that the rice protein OsPGDH-3 can effectively control the incidence of rice bacterial blight at a relatively low level.The application provides a new idea, strategy and gene resource for green prevention and control of rice bacterial blight.

The present application relates to a gene GmPMT for regulating soybean root nodule symbiotic nitrogen fixation and application thereof. Specifically, the present application relates to an isolated gene for regulating soybean root nodule symbiotic nitrogen fixation or nodule number, the coding region sequence of the gene is shown as SEQ ID NO:1 or a homologous sequence thereof has one or more substitutions, deletions and / or insertions of nucleotides compared with the sequence shown as SEQ ID NO:1. The present application also provides the use of the gene as described above in breeding soybeans with increased yield or changed nodule number, optionally, the changed nodule number is manifested as increased nodule number, nodule number per root and / or nodule number per unit root length, optionally, the changed nodule number is manifested as decreased nodule number, nodule number per root and / or nodule number per unit root length. The present application has great theoretical and application value for regulating soybean root nodule symbiotic nitrogen fixation or nodule number and its related application research.

This invention provides a gene encoding the E3 ubiquitin ligase BnaA02.SIP1 from Brassica napus and its application, belonging to the field of bioengineering technology. The nucleotide sequence of the BnaA02.SIP1 gene described in this invention is shown in SEQ ID NO:1. Specific primers were designed based on the coding region sequence of the BnaA02.SIP1 gene from Brassica napus, and a plant genetic transformation vector overexpressing the BnaA02.SIP1 gene was constructed using the constitutive expression promoter CaMV35S. The gene was then introduced into wild-type Arabidopsis thaliana and Brassica napus using Agrobacterium-mediated transformation, resulting in transgenic Arabidopsis thaliana and Brassica napus plants. The invention also verified that this gene plays a role in regulating salt tolerance in seedlings. This invention provides a theoretical basis and gene source for cultivating new salt-tolerant rapeseed varieties.

A primer pair, method, and use for rapidly identifying newborn nude mice. On the basis that a deletion mutation occurs in the base G at position 337 of a coding region of the Foxn1 gene in nude mice, a primer pair introducing an enzyme digestion site is designed according to sequence characteristics. Using the genome of a mouse to be tested as a template, the Foxn1 gene is amplified according to a PCR-RFLP method. The PCR product is then digested with a SmaI restriction endonuclease, and the digested product is genotyped by agarose gel electrophoresis. In addition, a reaction system that can be combined with lateral flow technology is screened, thereby achieving instant detection of the newborn nude mice. The method has the characteristics of simple operation, short time consumption, low cost, instant detection, etc.

The invention provides application of an FZD4 gene. The FZD4 gene is used for regulating osteogenic differentiation and mineralization of chicken bone marrow mesenchymal stem cells. The FZD4 gene in the chicken bone marrow mesenchymal stem cells is over-expressed and is used for promoting osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; or / and the FZD4 gene in the chicken bone marrow mesenchymal stem cells is knocked down to inhibit osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; the nucleotide sequence of the coding region of the FZD4 gene is as shown in SEQ ID NO. 1. According to the invention, the positive regulation effect of the FZD4 gene in osteogenic differentiation of chicken bone marrow mesenchymal stem cells is determined for the first time, the blank of a bone regulation mechanism of the poultry FZD4 gene is filled, and a new biological preparation target is provided for prevention and treatment of poultry bone diseases; the FZD4 gene can also be used as a molecular marker for breeding chicken varieties with high bone strength.

The invention relates to the field of biology, and particularly provides a replication-deficient influenza virus vaccine skeleton which comprises a PA gene, an HA gene, an NP gene, an NA gene, an M gene, an NS gene and a PB2 gene of an influenza virus. The PB2 gene is replaced into a PB1 gene coding region of a vaccine skeleton; the open reading frame of the original PB2 gene can be replaced by an exogenous gene, so that the exogenous gene is inserted into the vaccine skeleton and expressed. The skeleton has the advantages that the capacity is large, the antigen can be efficiently and stably expressed, the passage is stable, and the like. Meanwhile, the invention also discloses a replication-defective influenza virus as well as a preparation method and application thereof.

This invention discloses PtoNAC028 The gene is used to regulate the ratio of pectin monomers in poplar trees. The coding region of this gene has the nucleotide sequence shown in SEQ ID NO:1. This is achieved through overexpression or silencing of the gene in poplar trees. PtoNAC028 Genes capable of increasing or decreasing the proportion of lignin monomers in poplar provide key genes for the genetic improvement of poplar processing traits. This invention also discloses a method for cultivating poplars with a high lignin monomer ratio, through overexpression of [gene name missing] in poplar trees. PtoNAC028 The gene can significantly increase the proportion of lignin monomers in poplar trees, achieving targeted regulation of monomeric lignin metabolism, which helps to rapidly cultivate new poplar varieties with excellent wood properties and fast growth.

The invention provides a gene for regulating and controlling thermo-sensitive male sterility characters of plants and application of the gene. Specifically, the thermo-sensitive male sterility gene is a newly identified gene, mutation of a gene coding region of the thermo-sensitive male sterility gene causes generation of thermo-sensitive male sterility characters, and a new material is provided for Chinese cabbage male sterility molecular mechanism research and breeding.

The application discloses a universal adapter primer for amplifying the 5' end of cDNA and a 5' RACE method, and relates to the technical field of cDNA end amplification. The adapter primer sequence provided by the application can obviously reduce non-specific amplification bands after amplifying the 5' end of cDNA, and more target products are obtained. The kit and the 5' RACE method provided by the application are simple to operate, especially, the pre-mixed PCR reaction system can obviously reduce experimental operation steps and greatly shorten the cDNA amplification period. The 5' RACE method provided by the application has high cloning efficiency for unknown fragments. Through two rounds of PCR and several simple steps, the amplification of the 5' end unknown sequence can be completed, and complete 5' end sequence information of a coding region is obtained.