819 results about "Coding region" patented technology

The invention belongs to the technical field of biology, and provides a porcine epidemic diarrhea virus mRNA vaccine and application. The mRNA molecule comprises a coding region for coding the S protein of the PEDV; the PEDV is of a G2a type, and two proline mutations I1076P and L1077P are introduced into an S protein. The invention further discloses a DNA molecule. An S protein of PEDV; a recombinant plasmid; recombining the host cell; an mRNA vaccine formulation which can be used to prepare an antibody against the S protein of PEDV after immunizing an animal; the invention further discloses application of the mRNA molecule, the DNA molecule, the recombinant plasmid or the host cell to preparation of a medicine for preventing PEDV (Porcine Epidemic Diarrhea Virus) infection. The vaccine prepared by the invention is fast in antibody generation and high in protection rate, can generate high-level neutralizing antibodies in serum and breast milk of sows after the pregnant sows are immunized before parturition, has a remarkable protection effect on piglets, and can effectively prevent the piglets from being infected with PEDV (Porcine Epidemic Diarrhea Virus).

The invention relates to a gene GmHB1a participating in development of vascular bundles of soybean root nodules and application of the gene GmHB1a. Specifically, the invention relates to a separated gene participating in development of vascular bundles of soybean root nodules or regulation and control of the number of soybean nodules, and a coding region sequence of the gene is shown as SEQ ID NO: 1 or a homologous sequence of the gene. The invention also provides a method for regulating and controlling the nodulation number of the plant, which comprises the following steps: introducing the gene or a sequence for inhibiting the expression of the gene into a target plant cell or tissue to obtain a transgenic plant, the nodulation number of the transgenic plant is changed compared with that of the target plant, the plant is a leguminous plant, preferably soybean, and the nodulation number of the transgenic plant is changed. Optionally, the change in number of nodules is manifested as an increase in number of nodules and / or in number of single nodules, and optionally, the change in number of nodules is manifested as a decrease in number of nodules and / or in number of single nodules.

The invention provides an artificial nucleic acid molecule which is used for improving the expression quantity of target amino acid, polypeptide or protein. The artificial nucleic acid molecule at least comprises a target 5'untranslated region (UTR), a target coding region (CDS) and a target 3 'untranslated region (UTR). Wherein the sequence of the target 5 'UTR is one of the following sequences: 5' UTR of a high-expression gene and a 5 'UTR variant of the high-expression gene. The sequence of the target 3 'UTR is one of the following sequences: 3' UTR of a high-expression gene and a 3 'UTR variant of the high-expression gene. Optionally, the artificial nucleic acid molecule may further comprise, for example, a 5 '-end cap structure (Cap), a PolyA tail. The 5 'UTR and the 3' UTR have regulating effects on translation and stability of nucleic acid molecules, so that the 5 'UTR, the 3' UTR and variants thereof are selected from high-expression genes, the nucleic acid molecules can be further stabilized and are not easy to degrade, and the amount of protein or polypeptide obtained by translation of the nucleic acid molecules can be increased. The invention also provides methods for making, delivering, and using such artificial nucleic acid molecules, as well as the use of the artificial nucleic acid molecules for the treatment and / or prevention of related diseases or disorders.

The invention provides an SNP (Single Nucleotide Polymorphism) site of an ABO blood group variant, and an A subtype variant blood group identification kit and method, and relates to the technical field of biology. The SNP sites of the ABO blood group variant comprise the 155th c.155Ggt from an initiation codon of an ABO gene coding region, and the 155th c.155Ggt from the initiation codon of the ABO gene coding region; mutation of T. A substance for detecting the SNP site of the ABO blood group variant can be applied to preparation of an A-subtype variant blood group identification method and product so as to be effectively applied to rapid detection of patients and blood donors, the A-subtype variant blood group of an individual can be defined, and possible blood transfusion reaction can be prevented in time.

The invention provides an SNP (Single Nucleotide Polymorphism) site of an ABO blood group variant, and a B subtype variant blood group identification kit and method, and relates to the technical field of biology. The SNP (Single Nucleotide Polymorphism) sites of the ABO blood group variant comprise the 842th c.842Cgt from an initiation codon of an ABO * B.01 allele coding region of an ABO gene; mutation of T. A substance for detecting the SNP site of the ABO blood group variant can be applied to preparation of a B subtype variant blood group identification method and product, so that the substance can be effectively applied to rapid detection of patients and blood donors, the B subtype variant blood group of an individual can be defined, and potential possible blood transfusion reaction can be prevented in time.

The invention provides a UTR (Untranslated Region) sequence for improving mRNA (Messenger Ribonucleic Acid) stability and translation efficiency and application of the UTR sequence, and particularly provides an mRNA molecule which comprises a coding region for coding polypeptide or protein and a fragment thereof, a 5 'UTR sequence positioned at the upstream of the coding region and / or a 3' UTR sequence positioned at the downstream of the coding region, the 5 'UTR sequence is a nucleotide sequence as shown in SEQ ID NO: 1, and the 3' UTR sequence is a nucleotide sequence as shown in SEQ ID NO: 2; and / or, the 5 'UTR sequence is a nucleotide sequence as shown in SEQ ID NO: 3, and the 3' UTR sequence is a nucleotide sequence as shown in SEQ ID NO: 4. According to the present invention, the mRNA stability and the translation efficiency are improved through the new and optimized UTR sequence, and the UTR sequence can be used as the element for enhancing the RNA expression efficiency in the nucleic acid treatment drug or the mRNA vaccine so as to provide more and better choices for the mRNA therapy.

The invention relates to a gene GmHB21 participating in development of vascular bundles of soybean root nodules and application of the gene GmHB21. Specifically, the invention relates to a separated gene participating in development of vascular bundles of soybean root nodules or regulation and control of the number of soybean nodules, and a coding region sequence of the gene is shown as SEQ ID NO: 1 or a homologous sequence of the gene. The invention also provides a method for regulating and controlling the nodulation number of the plant, the method comprises the following steps: introducing the gene or a sequence for inhibiting the expression of the gene into a target plant cell or tissue to obtain a transgenic plant, the nodulation number of the transgenic plant is changed compared with that of the target plant, and the plant is a leguminous plant, preferably soybean. In addition, the invention also provides application of the gene or a sequence for inhibiting expression of the gene in cultivation of leguminous plants with changed nodulation number, such as soybean.

The invention provides a primer probe group for simultaneously detecting American strains and European strains of porcine reproductive and respiratory syndrome viruses, a detection method and application, and particularly belongs to the technical field of porcine pathogen detection products. The invention provides a primer probe group for simultaneously detecting American strains and European strains of porcine reproductive and respiratory syndrome viruses. The primer probe group comprises universal forward primers PRRSV1-F and PRRSV2-F for detection, reverse primers PRRSV1-R and PRRSV2-R for detection and a universal probe PRRSV-P. A target gene detected by the primer probe group is a 3'non-coding region gene (3 'UTR) of a porcine reproductive and respiratory syndrome virus genome, and the primer probe group can realize efficient detection of American strains and European strains of the porcine reproductive and respiratory syndrome virus, and is simple and convenient in detection, high in sensitivity, good in specificity, high in speed, low in false positive and capable of realizing high-throughput detection.

The invention discloses an mRNA non-coding region sequence optimization method and system and electronic equipment, and the mRNA non-coding region sequence optimization method comprises the steps: constructing an initial candidate library according to a target protein; inputting the initial candidate library into a pre-trained mRNA sequence optimization model to obtain a prediction data set; performing multi-dimensional scoring and sequence optimization on the prediction data set to obtain a sequence recommendation group; performing biological verification on the sequence recommendation group to obtain an optimized mRNA sequence; wherein the prediction data set comprises a sequence ID, a sequence content, a prediction TE score and a confidence interval. According to the method, the translation efficiency of the mRNA sequence can be efficiently and accurately predicted, the candidate sequence with high expression potential is screened out, meanwhile, the consumption of computing resources is reduced, and the overall design cost is reduced.

The invention relates to the technical field of bioinformatics, in particular to a biological information analysis system based on a large model technology, which comprises a data analysis calibration module, a problem disassembly module, an analysis task arrangement module, a result mapping module and a feedback iteration module. According to the method, genome comparison and clinical phenotype timestamps are dynamically calibrated, time sequence dislocation deviation is eliminated, base complementary pairing is combined with protein network anomaly screening, low-abundance collaborative variation capture is enhanced, genotype-phenotype discrete distribution quantifies and unifies multi-modal data benchmark, and the problem of multi-source heterogeneous standardization deficiency is solved; the method comprises the following steps: classifying and integrating pathogenic gene semantic weights by structural variation, balancing a statistical threshold and a biological function, dynamically optimizing an analysis sequence, synchronously covering a key mutation region, improving function annotation of a non-coding region, integrating gene expression clustering and protein network topology in a three-dimensional distribution manner, breaking through two-dimensional space limitation, performing closed-loop feedback to correct a threshold iteration elimination rule, and finally obtaining a high-quality gene expression cluster. And the genetic heterogeneity false positive rate is reduced.

The invention belongs to the technical field of plant breeding and biology, and particularly relates to how to reduce the cadmium content of rice grains through a gene OsCT1 and application of the gene OsCT1 to creation of new germplasm of low-cadmium-accumulation rice. The invention discloses a nucleotide sequence of a gene OsCT1 coding region, a coded protein sequence, a CRISPR-Cas9 knockout target site nucleotide sequence, a nucleotide sequence of a knockout mutant and a protein sequence of the knockout mutant, as shown in an ST.26 standard sequence nucleotide or amino acid sequence table. After the gene OsCT1 is knocked out by utilizing a CRISPR-Cas9 technology, the cadmium content of grains of the OsCT1-knocked-out transgenic plant is obviously lower than that of a wild type variety. In addition, in the seedling stage, the plant height and the root length of the OsCT1-knocked-out transgenic plant are remarkably larger than those of a wild type variety; after the OsCT1 gene is subjected to overexpression, the grain cadmium content of an overexpressed transgenic plant is slightly higher than that of a wild type variety. In addition, in the seedling stage, the plant height and the root length of the overexpressed OsCT1 transgenic plant are obviously smaller than those of a wild type variety. According to the invention, the OsCT1 gene can be utilized to create a novel rice germplasm with low cadmium content in grains.

The invention provides a rice defense gene LOCOs02g41680 and application of the rice defense gene LOCOs02g41680 in bacterial leaf blight resistance, and relates to the technical field of modern biotechnology gene engineering. The rice defense gene LOCOs02g41680 disclosed by the invention has a nucleotide sequence as shown in SEQ ID NO.1, and consists of 2643 nucleotides; 2, the sequence length of the coding region sequence is 2142 nucleotides, and the coding region sequence is shown in SEQ ID NO. A protein with 713 amino acids is coded, and the amino acid sequence is shown in SEQ ID NO.3. The rice defense gene LOCOs02g41680 disclosed by the invention can be used for creating a rice disease-resistant material for resisting bacterial blight, the disease resistance of the rice can be improved when the rice defense gene LOCOs02g41680 is applied to the rice, and valuable gene resources can be provided for breeding new varieties of the rice for resisting the bacterial blight.

The invention discloses application of an upstream non-coding region uORF of an ABA2 gene in regulation and control of crop agronomic traits, in particular to application in improvement of pre-harvest sprouting resistance of rice and cultivation of pre-harvest sprouting resistant rice strains, and belongs to the technical field of gene engineering. The invention finds that uORF in the upstream non-coding region of the crop ABA2 gene can inhibit the translation level of downstream ABA2 protein on the basis of not influencing the transcriptional level, so that the uORF can participate in regulation and control of crop agronomic traits in which ABA participates in regulation and control, such as improvement of pre-harvest sprouting resistance of rice. Therefore, the upstream non-coding region uORF of the crop ABA2 gene can be modified through a hybridization or gene editing technology, so that the purpose of regulating and controlling crop agronomic traits in which ABA participates in regulation and control is achieved, for example, site-directed mutagenesis is performed on an initiation codon of the upstream non-coding region uORF of the rice ABA2 gene, and the pre-harvest sprouting resistance of rice is improved.

The invention relates to an SNP molecular marker related to litchi fruit weight and application thereof, and belongs to the technical field of molecular biology. The SNP molecular marker related to the litchi fruit weight is based on a seedless litchi reference genome, the SNP molecular marker is located in a litchi SL05G0138630 gene coding region, and is located at the 27564818th basic group of a litchi chromosome 5, and the basic group is A or G. The application finds that a non-synonymous SNP site in a coding region of the litchi SL05G0138630 gene is closely associated with the litchi fruit weight phenotype, the variety with the weight of the litchi fruit can be efficiently identified by detecting the polymorphic site, the invention further discloses an identification method for the molecular level of the variety with the weight of the litchi fruit, and the molecular level of the variety with the weight of the litchi fruit can be identified by an SNP-based typing method. And cheap and accurate gene SNP typing can be achieved, so that the purpose of efficiently and accurately identifying the weight variety of the litchi fruit is achieved.

The present invention belongs to the technical field of biological prevention and treatment for viral diseases. Disclosed in the present invention are a tomato disease-resistant gene mutant, and the use thereof in the prevention and treatment of ToBRFV. It is found in the present invention that mutating the nucleotide at position 1927 of the coding region sequence of tomato Tm-22 gene from G to A, or mutating the amino acid at position 643 of the LRR domain of a protein that is encoded by tomato Tm-22 gene from glycine to arginine can remarkably reduce the accumulation level of ToBRFV capsid protein (CP). The gene (named Tm-22-Mut5) can serve as a novel ToBRFV resistant gene, and also retains the resistance to TMV, ToMV and ToMMV. The Tm-22-Mut5 and a pre-screened Tm-22-Mut3-1 mutant (tyrosine at position 767 of the LRR domain thereof is mutated to phenylalanine) undergo combinatorial mutagenesis to obtain a mutant Tm-22-Mut6. Analysis shows that the Tm-22-Mut6 can remarkably reduce the accumulation level of the ToBRFV capsid protein. Compared with the pFGCTm-22-Mut3-1 mutant obtained by screening in the previous research and the newly obtained mutant Tm-22-Mut5, the Tm-22-Mut6 has further improved resistance to ToBRFV.

The embodiment of the invention discloses an mRNA sequence generation method and device, a model training method and device and electronic equipment. The method comprises the steps that a target protein sequence, a target species text corresponding to the target protein sequence and target translation efficiency corresponding to the target protein sequence are obtained; determining a first target embedding corresponding to the target species text, determining a second target embedding corresponding to the target protein sequence, and determining a third target embedding corresponding to the target translation efficiency; splicing the first target embedding, the second target embedding and the third target embedding to obtain a fourth target embedding; a sequence generation model is called to map the fourth target embedding, a target mRNA sequence is generated, and the target mRNA sequence comprises a first coding region, a first untranslated region and a second untranslated region; according to the embodiment of the invention, the accurate and complete target mRNA sequence can be obtained, the application effect of the target mRNA sequence can be improved, and the method can be widely applied to scenes such as cloud technology, artificial intelligence and smart medical treatment.

The invention relates to a mytilus coruscus byssus adhesion protein gene Mfp5 coding region SNP marker and application thereof, the mytilus coruscus byssus adhesion protein gene Mfp5 coding region SNP marker comprises a gene sequence numbered as an McMfp5 coding region, the nucleotide sequence of the gene sequence is shown as SEQ ID No.1, an SNP marker primer pair is designed based on the sequence, three SNP markers numbered as McMfp51, McMfp52 and McMfp53 are obtained through sequencing, and the primer sequences of the three SNP markers are shown as SEQ ID No.2 and SEQ ID No.3 in sequence. The primer designed aiming at the gene sequence of the McMfp5 coding region is used for SNP (Single Nucleotide Polymorphism) marker typing, is high in specificity, stable in amplification and high in polymorphism, can be used for genetic diversity analysis, germplasm identification and marker-assisted selection breeding of mytilus coruscus populations, and provides a powerful tool for genetic breeding of mytilus coruscus.

The invention provides application of a P8 gene, or a P8 gene coding protein, or a recombinant vector or host bacteria containing a P8 gene coding region in improvement of GFP (Green Fluorescent Protein) expression quantity and / or inhibition of degradation of an mRNA (Messenger Ribonucleic Acid) attenuated substrate taking GFP as a reporter gene. The research finds that the P8 has a silence inhibition function, can inhibit degradation of a nonsense mediated mRNA decay substrate taking GFP as a reporter gene and degradation of an endogenous nonsense mediated mRNA decay (NMD) substrate of arabidopsis thaliana, can improve the mRNA expression quantity of AT1G0106020, RPS6, SMG7, AT5G35490, AT5G64430, AT1G36730, AT4G3900 and AT5G22570, and can reduce the expression quantity of AT1G72450 and AT2G400, and the research result lays a foundation for clarification of the function of the P8.

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA1 gene, an expression vector and application of the CeNF-YA1 gene in vegetable fat regulation and control. The invention provides the CeNF-YA1 gene for the first time, the nucleotide sequence of the CeNF-YA1 gene is as shown in SEQ ID NO.1, the full length of a coding region of the CeNF-YA1 gene is 792 bp, a series of carriers containing the CeNF-YA1 gene, such as subcellular localization, yeast hybridization and plant overexpression, are constructed for the first time, and meanwhile, the CeNF-YA1 protein is proved to have a transcription activation function, is localized in a cell nucleus and conforms to the basic characteristics of transcription factors. It is found for the first time that overexpression of the CeNF-YA1 gene can significantly improve the grease content of arabidopsis seeds and leaves; therefore, the technical scheme provided by the invention can be used for improving the oil content of the plant seeds and the nutritional tissues, and shows the application prospect in improving the oil content of the seeds and the nutritional tissues.

The invention provides a lysine artificial operon and application thereof. The gene sequence of the artificial operon comprises a promoter, a lysC gene for coding aspartate kinase, an asd gene for coding aspartate semialdehyde dehydrogenase, a dapA gene for coding dihydropicolinic acid synthetase and a dapB gene for coding dihydropicolinic acid reductase, the ddh gene is used for coding diaminopimelate dehydrogenase; the lysA gene is used for coding diaminopimelate decarboxylase; except for the first lysC gene, sequences of RBS ribose binding sites of other genes are RBS ribose binding sites translated after gene transcription by intercepting 20-26nt DNA sequences at the upstream of a coding region ATG (adenosine triphosphate); the RBS ribose binding site is connected in front of the corresponding gene.

The invention discloses a barcode array repeatable chip and a preparation method and application thereof, and belongs to the technical field of gene sequencing and tissue cell sample analysis. The chip comprises a chip substrate, a universal primer probe and a bar code probe, the bar code probe comprises a Nick region, a capture region, a position bar code region and a molecular bar code region which respectively execute corresponding functions, and particularly, the Nick region is arranged, so that the chip can generate a repeatable bar code chip through SDA reaction, and the chip can be used for detecting the bar code of the chip. The bar code chip can be repeatedly used through DNA probe hybridization, polymerization extension and polymerization strand displacement. The chip has a simple structure, is easy to process, and can be used, so that the preparation of the bar code chip is realized at extremely low cost.

The disclosure includes methods and apparatuses that includes processing circuitry that derives a chroma prediction mode of a chroma block in a chroma coding region based on luma coding information of a luma block associated with a position in a reconstructed luma area that is collocated with the chroma block. The chroma coding region is partitioned separately from a collocated luma coding region including the reconstructed luma area. The luma coding information indicates that the luma block is coded with a first coding mode using a first luma BV (an IBC mode or an IntraTMP mode) and a second coding mode. The processing circuitry derives a chroma BV of the chroma block based on a luma BV of the luma block and reconstructs the chroma block based on the chroma prediction mode including the first coding mode and the second coding mode.

The invention provides an application of a TGBp1 gene, or a TGBp1 gene coding protein, or a recombinant vector or host bacteria containing a TGBp1 gene coding region in down-regulation of the MeGRXC3 expression level. Researches find that TGBp1 and MeGRXC3 interact with each other, so that the growth ability of yeast in an SD / -Leu / -Trp / -His / -Ade auxotroph culture medium can be improved, the expression of MeGRXC3 can be down-regulated, the active oxygen content of tobacco leaves for co-expression of TGBp1 and MeGRXC3 is improved, and the growth ability of the yeast in the SD / -Leu / -Trp / -His / -Ade auxotroph culture medium is improved. Research results lay a theoretical foundation for breeding of common cassava mosaic virus disease resistance.

The invention provides a varicella-zoster virus mRNA (messenger Ribonucleic Acid) vaccine and application thereof. The invention firstly provides an mRNA molecule, a coding region sequence of the mRNA molecule comprises a nucleotide sequence for coding an amino acid sequence as shown in SEQ ID NO: 1, and the coding region sequence preferably comprises a nucleotide sequence as shown in any one of SEQ ID NO: 2 to SEQ ID NO: 11, or a nucleotide sequence which has at least 93% similarity with the nucleotide sequence as shown in any one of SEQ ID NO: 2 to SEQ ID NO: 11 and codes the same amino acid sequence. When the mRNA molecule is used for preparing the vaccine, the production cost of the vaccine can be reduced, the physicochemical property of the vaccine is stable, the protein expression is improved to enhance the immune effect of the vaccine, and the humoral immunity and cellular immunity effects of the VZV-gE specificity are enhanced.

The invention provides an application of an AC2 gene, or an AC2 gene coding protein, or a recombinant vector or host bacteria containing an AC2 gene coding region in reducing the expression level of AtSGS3, and / or reducing the expression level of MeSGS3, and / or reducing an SGS3 / RDR6 compound. Researches find that SLCMV AC2 interacts with AtSGS3 and MeSGS3, the growth ability of yeast in an SD / -Ade / -His / -Leu / -Trp auxotroph culture medium and an SD / -Ade / -His / -Leu / -Trp auxotroph culture medium added with 3-AT can be improved, expression of AtSGS3 and MeSGS3 can be down-regulated, an SGS3 / RDR6 compound can be reduced, and a research result lays a foundation for clarification of a molecular mechanism of SLCMV AC2 regulation and control of host RNA silencing.

The invention relates to a high-throughput cis-acting element screening carrier and a screening method. Specifically, the invention provides a plasmid vector system containing bar codes, each bar code in the system is in one-to-one correspondence with a candidate cis-acting element, and the activation multiple of the candidate cis-acting element can be obtained by measuring the abundance of the bar codes; in order to eliminate the influence of a bar code on the vector on a detection result, an exogenous intron which can be cut off during transcription is inserted into a coding region in the vector and is used for distinguishing vector DNA and RNA obtained by transcription. The screening method of the biological cis-acting element has high efficiency, wide applicability and high throughput, and has outstanding application value in biological research and breeding.

The invention relates to the technical field of plant genetic engineering, in particular to a ginkgo GbGRF2 gene and application of a GbGRF2-GbGIF2 fusion gene of the ginkgo GbGRF2 gene in plant tissue culture. According to the invention, the GbGRF2 gene coding region full length of the GRF gene family of gingko is successfully cloned from gingko, and the gene presents a specific expression mode in the regeneration process of gingko callus; the overexpression of the fusion gene of GbGRF2 and GbGRF2-GbGIF2 can significantly promote the regeneration efficiency of adventitious buds in the in-vitro culture process of plants; the gene provided by the invention has important theoretical significance and application potential in a plant tissue regeneration regulation network, and plays a key role in improving in-vitro regeneration efficiency of gymnosperm.

The invention discloses an application of a multidrug-resistant efflux transporter gene OsMRET1 in regulation and control of chalkiness traits of rice. A nucleotide sequence of a coding region of the gene is shown as SEQ ID No.1, and a coded amino acid sequence is shown as SEQ ID No.2. The invention further discloses an application of the multidrug-resistant efflux transporter gene OsMRET1 in regulation and control of chalkiness traits of rice. It is found for the first time that the gene OsMRET1 can regulate and control formation of chalkiness, an OsMRET1 gene knockout mutant is created through a gene editing technology, an OsMRET1 gene overexpression strain is obtained through a transgenic technology, it is verified that the OsMRET1 gene is a new gene for regulating and controlling rice chalkiness, and overexpression of the gene can reduce rice chalkiness and improve rice chalkiness. And a new gene resource and a technical route are provided for rice quality improvement.

The present invention provides a site-directed mutated influenza virus gene non-coding region nucleic acid fragment and its application in improving the expression efficiency of mRNA vaccines. The provided site-directed mutated influenza virus gene non-coding region nucleic acid fragment has a sequence of SEQ ID NO: 3. The present invention also provides an mRNA fragment for translating and expressing in a host, which contains a coding frame for encoding a target protein, and the 5′UTR and / or 3′UTR of the mRNA fragment is the site-directed mutated influenza virus gene non-coding region nucleic acid fragment. In view of the problems that the existing mRNA vaccine has low translation efficiency, relatively insufficient activation of the immune system, and difficulty in producing the expected immune protection effect, the present invention provides a method for site-directed mutagenesis of a non-coding region derived from an influenza virus M gene fragment, which can be used as the 5′UTR and / or 3′UTR of the target expression sequence, and can improve the translation efficiency of the target mRNA.

The invention provides a separated gene for regulating and controlling the nodulation number of leguminous plants. A coding region sequence of the gene is shown as SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 3 or a homologous sequence of the SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 3. The invention has great theoretical and application values for leguminous plant breeding and related application research thereof.