6 results about "Gymnosperm" patented technology

The invention belongs to the field of gene engineering, and relates to a plant genome directional editing tool based on transposon-coded nuclease. The invention aims to solve the technical problem that the application range of a plant genome editing tool based on CRISPR-Cas9 and Cas12a is limited to a great extent due to the fact that CRISPR-Cas9 and Cas12a are large in protein size and difficult to deliver. The invention provides transposon nuclease suitable for plant genome editing. The transposon nuclease is IsDge10, IsAam1 or enIscB subjected to codon preference optimization; and a gene editing system containing the three transposon nuclease is constructed. The gene editing system can be suitable for editing a coding region, a non-coding region and the like; the method can be widely applied to monocotyledonous plants, dicotyledonous plants, gymnosperm and the like.

The invention discloses a nucleotide sequence of a larch endogenous strong promoter Pro-LarGRP1A and an application of the larch endogenous strong promoter Pro-LarGRP1A. The invention relates to a method for creating and cultivating woody gymnosperm plants such as larch with precisely improved target agronomic characters such as biomass, disease resistance, insect resistance, stress resistance and the like through genetic transformation of a carrier expressed by a target gene driven by a Pro-LarGRP1A promoter sequence and a method for creating and cultivating the woody gymnosperm plants such as the larch with precisely improved target agronomic characters such as biomass, disease resistance, insect resistance, stress resistance and the like. The implementation effect shows that compared with the known conventional plant expression vector, the expression vector constructed by taking the nucleotide sequence of the Pro-LarGRP1A promoter region disclosed by the invention as the promoter element is used for transforming a larch receptor material through an agrobacterium-mediated genetic transformation method, a target gene in a positive transformed strain can be efficiently expressed, and the expression vector has the advantages that the expression vector is simple and convenient to construct, and the application prospect is wide. And the gene expression level is more than 8 times that of the conventional expression vector. The nucleotide sequence of the larch endogenous strong promoter Pro-LarGRP1A and the application of the larch endogenous strong promoter Pro-LarGRP1A disclosed by the invention have an important application value in efficient and low-cost cultivation of new germplasm, new materials and new varieties of woody gymnosperm plants such as high-biomass, high-volume, high-stress-resistance and high-quality larch.

This invention belongs to the field of computer image processing and computational wood science, specifically relating to an intelligent batch identification and analysis system for cells in cross-sections of gymnosperm wood. The system includes sequentially connected modules for image input, preprocessing, cell instance segmentation, unique ID generation and management, batch calculation of geometric parameters, and result output and interactive filtering. The method involves: after preprocessing the input image, using instance segmentation based on traditional image processing algorithms to batch identify cell outlines; assigning a unique ID to each cell and establishing a mapping; calculating the cell lumen area, diameter, and wall thickness in batches based on the mapping relationship; and finally, performing visualization and interactive filtering. This invention, by integrating batch parallel processing and cell instance-ID mapping management, achieves minute-level fully automated identification and measurement of massive numbers of cells in wood cross-sections, improving efficiency by hundreds of times compared to manual serial operations, and supports fine-grained data management and statistics.

The present application relates to the technical field of plant genetic engineering, and particularly relates to a ginkgo biloba L. GbGRF2 gene and application of a ginkgo biloba L. GbGRF2-GbGIF2 fusion gene in plant tissue culture; a full-length coding region of a ginkgo biloba L. GRF gene family GbGRF2 gene is successfully cloned from ginkgo biloba L., the gene presents a specific expression mode in a ginkgo biloba L. callus regeneration process; overexpression of the GbGRF2 and GbGRF2-GbGIF2 fusion gene can significantly promote regeneration efficiency of adventitious buds in an in-vitro culture process of a plant; the gene provided by the present application has important theoretical significance and application potential in a plant tissue regeneration regulation network, and plays a key role in improving in-vitro regeneration efficiency of gymnosperms.

This invention provides a method for isolating cell nuclei from *Pinus tabuliformis* tissue, relating to the field of plant molecular and cell biology. The method uses *Pinus tabuliformis* callus tissue as explants, releases protoplasts using a specific enzymatic hydrolysis solution, lyses them using a specific lysis buffer, and finally filters and centrifuges to obtain *Pinus tabuliformis* cell nuclei. Using this invention, cell nuclei suitable for single-cell and histone modification library construction and sequencing can be rapidly isolated, providing a foundation for research on single-cell nuclear genetic material transcription analysis, tissue-specific analysis, and cell fate determination in gymnosperms, and offering research ideas for the future use of cell nuclei in coniferous genetic engineering.

The application discloses a method for identifying the source of crude oil organic matter and a chart thereof. The method comprises the following steps: separating an aromatic hydrocarbon component from a sample; obtaining a characteristic ratio for characterizing dimethylphenanthrene isomer distribution by gas chromatography-mass spectrometry, and the ratio is denoted as R value; determining the carbon isotope value of 1,7-DMP by gas chromatography-isotope ratio mass spectrometry, and calculating the logarithm value with 2 as the base, and the value is denoted as L value; constructing a two-dimensional identification chart by using the R value and the L value of standard samples with known sources; and finally, projecting the measured value of the sample to be measured into the chart, and identifying the source type of the parent material according to the region where the sample falls. The stable carbon isotope information and the molecular distribution information of 1,7-DMP are combined, the defects that the traditional biomarkers are interfered by thermal maturity and biodegradation are effectively overcome, different organic parent materials such as aquatic algae, angiosperms, herbaceous plants and gymnosperms can be finely distinguished, and a reliable technical means is provided for oil-source correlation under complex geological conditions.