44 results about "Gymnosperm" patented technology

Novel oil extracts from Angiosperm and Gymnosperm plants and other-plant biomass from human, veterinary, birds, aquatic species, microbial and mycological sources useful in human, veterinary and agricultural, mycological and microbiological applications are described. Methods of preparation of these extracts in oil and methods of application and administration are also described.

The invention discloses a method for extracting RNA from gymnosperm tissues. The method includes the following steps: 1) extracting buffer solution by RNA to treat plant materials; 2) protein removal: extracting by equal-volume chloroform / isoamylol for twice; 3) using LiCl to deposit RNA and SSTE to dissolve back RNA; 4) glycan removal: adding absolute ethyl alcohol and potassium acetate to the dissolved solution acquired in step 3) and depositing glycan in ice bath; 5) extracting chloroform / isoamylol again; 6) depositing RNA and DEPC H2O again to dissolve back RNA. In order to remove DNA, the following steps can be also conducted: a) using DNase without RNase pollution to remove DNA; b) using chloroform / isoamylol to extract and remove DNase; (c) repeating step 6). Wherein, RNA extract and buffer solution contains 100mmol / L Tris HCl(pH 8.0), 2g / 100mlCTAB, 2g / 100mlPVP, 2mol / LNaCl and 25mmol / L EDTA and is added with dithiothreitol with final concentration of 10mmol / L and 2 percent (volume percentage) of beta-mercaptoethanol. Therefore, the invention solves the interference of glycan and polyphenol and the like and acquires high-purity RNA.

An immune-fluorescence labeling method of micro-pipe frame at pollen tube on gymnosperm includes fixing pollen tube of gymnosperm on fixing liquid for 40-60min, washing pollen tube, placing pollen tube in enzyme solution for carrying out enzymolysis of 30-40 min under temperature of 20-30deg.c, washing pollen tube, setting pollen tube in Triton X-100 to penetrate there for 1-3h, washing pollen tube, adding anti-beta-tubulin single clone antibody to carry out hatching for 1-3h, washing pollen tube, adding fluorescence label to carry out shielded hatching for 1-3h for making micro-pipe frame be immune-fluorescence labeled.

The invention discloses a labeling method of an apple pollen tube microfilament. The labeling method comprises the following steps: cultivating apple pollen, curing the apple pollen, performing enzyme treatment on the apple pollen, treating the apple pollen with a surfactant, performing dye labeling, and performing scanning and imaging. The labeling method has the beneficial effect that the labeling method of gymnosperm pollen tube microfilament is referred and improved on the basis of the labeling method of pear pollen tube microfilament, dyes enter cells easier through curing, enzymolysis of cell walls and the surfactant, the interference of cell outer wall autofluorescence due to insufficient enzymolysis as TRITC-Phalloidin is applied are avoided when FITC-Phalloidin labeling is applied, so that relatively complete apple pollen tube microfilament can be stored; moreover, the labeling method is efficient and quick, can be used for obtaining relatively clear and bright pictures, thus filling up the blank of the apple pollen tube microfilament labeling, providing reference for labeling microfilament in rosaceae and angiosperm pollen tube, and achieving a high application value.

The invention provides a pine pollen extract for treating fatty liver as well as a preparation method and an application thereof, relating to the technical field of extracting effective substances from gymnosperm pine pollen. The preparation method comprises the following steps: the pine pollen is subjected to wall breaking at a low temperature, and impurities in the pine pollen are removed; the pine pollen is extracted with ultrasonic after the pine pollen is immersed in normal-pressure normal-temperature water; solid impurities are centrifugally removed to obtain an aqueous solution; and a gymnosperm pine pollen aqueous extract with the molecular weight lower than 3,000D is separated at the environmental temperature of between 1 and 5 DEG C to obtain the gymnosperm pine pollen aqueous extract for treating the fatty liver. The pine pollen aqueous extract can be used for general health-care for children, and can prevent occurrence of fatty liver. The pine pollen aqueous extract can be used for preparing an oral preparation for treating the fatty liver, has a good absorption effect and low price, and also can be used for preparing an injection preparation for treating the fatty liver, which can be absorbed quickly, has strong treatment pertinence.

The invention relates to a torreya grandis seed after-ripening treatment method for promoting embryo development, used for gymnosperm taxaceae torreya.. Pearlite or river sand is soaked by clear water under the condition that no water is dropped when the pearlite or the river sand is held by hands and a soaked pearlite or river sand layer is paved at the bottom of a container, then torreya grandis seeds are placed in a layer way, and the soaked pearlite or river sand layer is covered on the torreya grandis seeds, and then other seeds are placed again, and then the soaked pearlite or river sand layer is covered on the seeds by parity of reasoning until the upper part of the container is covered by the pearlite or river sand layer; then poikilothermic treatment is carried out for 90-100 days; inspection is carried out and the pearlite or river sand is maintained to be moist, and no water is accumulated; after the seeds are subjected to after-ripening treatment for 90 days by adopting the method, not only reaches embryo development rate up to more than 95%, and dissection shows that torreya grandis grow to a fresh green mature embryo with the length of being about 2cm from a flavescent young embryo with the length of being about 0.001cm and the size same as that of a needle point, and the mature embryo almost penetrates trough the whole torreya grandis seed; and secondly, the torreya grandis seed after-ripening treatment method disclosed by the invention is applicable to sowing, raising seedling and tissue culture of the mature embryo, cotyledons, embryo, embryonic axis and endosperm of the torreya grandis and is also applicable to all torreya plants in the world.

The invention discloses a method for fluorescence labeling of gymnosperm pollen tube fibril bone and its application and is to provide a method for fluorescence labeling of gymnosperm pollen tube fibril bone and the application of this method in microscope inspection of gymnosperm pollen tube fibril bone. The said labelling method includes the following steps: 1) fixing the gymnosperm pollen tube with 40-60mM Pipes cushion solution containing 2-4 % cavaform; 2) washing the pollen tube; 3) placing the pollen tube in 0.2-0.3 % glycerin to infiltrate for 30-60min; 4) washing the pollen tube; 5) dyeing the pollen tube and avoiding the sun with 150-250nM phalloidine by fluorescence labeling for 0.5-1.5h and fluorescence labeling the fibril bone. The invention is of simple operation and is quick, has small damage to the cells, and has good labeling effect, which will play an important role in study gymnosperm pollen tube cell bone and have a high actual application value.

This invention discloses a method for preparing electrophoretic sample of gymnosperm pollen tube protein. The method comprises: (1) suspending gymnosperm pollen tube powder in trichloroacetic acid solution, placing at -20~-4 deg.C, centrifuging, collecting the precipitate, resuspending the precipitate in trichloroacetic acid solution, placing at -20 deg.C for 2-16 h, centrifuging, collecting the precipitate, washing with 80% acetone solution, and removing acetone to obtain crude extract of gymnosperm pollen tube protein; (2) dissolving in pyrolytic buffer solution, centrifuging, collecting to supernatant to obtain the electrophoretic sample of gymnosperm pollen tube protein. The electrophoretic sample of gymnosperm pollen tube protein can be used for reversible electrophoresis, and is rapid, simple and can provide high quality electrophoretic pictures.

The invention specifically provides a mechanical device using electrical stimulation to separate earthworms from cultivation soil thereof, solving the current problem that the efficiency of separating earthworms from cultivation soil thereof is low, living earthworms are easy to be damaged and cleaning operation is relatively hard to perform. A feeding assembly comprises separation column frames. Clearance support rods are arranged among adjoining separation column frames. The bottom end of the lowermost layer of the separation column frames is provided with a loading material plate. An electric expelling assembly comprises a fixing column. A cone-shaped electric expelling hollow part is arranged on the fixing column. The outer side of the cone-shaped electric expelling hollow part is provided with an embossing-shaped structure of gymnosperm cycas root surface. An earthworm separation mechanism comprises a hollow rotating shaft. The top end of the hollow rotating shaft is connected with a disc-shaped material dropping plate. The disc-shaped material dropping plate is provided with a dumping opening one. The bottom end of the hollow rotating shaft is provided with a motor. The output end of an electronic control system is connected with the input ends of the motor and the cone-shaped electric expelling hollow part. A dumping plate is aslant arranged in a cabinet. The mechanical device achieves the purpose of quickly separating earthworms from cultivation soil thereof.

In angiosperm and gymnosperm plants, overexpressing a SAMdc nucleotide sequence can decrease lignin content and, for plants with woody tissue, increase wood density.

A flooring board having a plurality of layers may have a sufficiently high modified Janka hardness rating for use in general flooring applications. The flooring board may comprise a fiberboard core layer having a thin veneer layer of a natural wood species adhered to a surface of the core layer. The veneer layer may be a gymnosperm (softwood) or angiosperm (hardwood) having a low Janka hardness rating. Surprisingly, the flooring board has a high modified Janka hardness rating significantly higher than the Janka hardness rating of the natural wood species and sufficient for use in general flooring applications when the veneer layer is sufficiently thin.

The present invention discloses a method for detecting gymnosperm pollen tube interior antigen. Said method includes the following steps: (1), using phosphoric acid buffer solution containing paraformaldehyde to fix pollen tube, using zymolysis solution containing cellulose and macerozyme to zymolyze gymnosperm pollen tube, using Triton X-100 or NP-40 aqueous solution whose mass percentage concentration is 0.2-1% to wash the pollen tube; and (2), making the pollen tube treated by step 1 and the correspondent antibody for resisting the antigen to be tested undergo the process of immunofluorescence reaction, if the fluorescence is detected, the gymnosperm pollen tube interior contains antigen to be tested and can obtain the location condition of said antigen to be tested.

The invention belongs to the field of plant gene editing, and particularly relates to a genome editing expression cassette, a vector containing the expression cassette and application of the vector. The technical problem to be solved by the invention is that no applicable efficient gene editing system exists in plants, especially gymnosperms at present. According to the technical scheme for solving the technical problem, the genome editing expression cassette is provided, the genome editing expression cassette comprises the following elements: a promoter MarPE004, a Cas9 protein coding gene and an sgRNA cloning and transcription unit, and the promoter MarPE004 drives the Cas9 protein coding gene and the sgRNA cloning and transcription unit to express. The expression framework and the corresponding vector provided by the invention can realize efficient gene editing in plants. Experiments show that the gene editing efficiency in the larch protoplast can reach 72.5%, meanwhile, the gene editing gene has high gene editing activity when PAM is NGG, NAG, NGT and other PAM sites, and the gene editing gene has wide application prospects.

A flooring board having a plurality of layers may have a sufficiently high modified Janka hardness rating for use in general flooring applications. The flooring board may comprise a fiberboard core layer having a thin veneer layer of a natural wood species adhered to a surface of the core layer. The veneer layer may be a gymnosperm (softwood) or angiosperm (hardwood) having a low Janka hardness rating. Surprisingly, the flooring board has a high modified Janka hardness rating significantly higher than the Janka hardness rating of the natural wood species and sufficient for use in general flooring applications when the veneer layer is sufficiently thin.

The present invention relates to the regulation of reproductive development, particularly to the genetic ablation of reproductive tissues in angiosperm and gymnosperm species. Reproductive-preferred promoters, regulatory elements, and cytotoxic nucleotide sequences are disclosed herein, as are constructs and methods for genetic ablation.

The invention discloses gymnosperm isolated culture method and the special using culture medium. The culture medium receipt is that: cane sugar 120-150g, boric acid 0.1-0.2g and calcium chloride 0.1-0.3g. They are settled to 1000 ml by water. The culturing method is that the gymnosperm pollen is hydrated for 20-30 minutes under room temperature, then it is cultured in the said medium for 6-72 hours, finally the pollen tube is got after pollen sprouting. The essential environment is supplied for pollen sprouting and growth. The culture by this invention is not only fast and easy, but also good in culturing result. A great deal of gymnosperm pollen tube with vitality can be got, and there is great practical application value.

The invention discloses an establishment method of a high efficient transient transformation system of exogenous genes of cunninghamia lanceolata, and belongs to the technical field of plant cytology.The method includes the following steps that cunninghamia lanceolata stem segments are subjected to digestion and enzymolysis through an enzymolysis approach to release protoplasts, the protoplasts are purified, PEG mediates the transformation of exogenous gene plasmid DNA into the protoplasts, high efficient transient transformation of the exogenous genes in the gymnosperm, namely cunninghamia lanceolata, is achieved, and the transformation efficiency of the cunninghamia lanceolata is improved greatly, and the method provides a technical support for molecular biology research and genetic transformation of main candidate genes of cunninghamia lanceolata.

The invention provides a pine pollen aqueous extract for treating premature ovarian failure of the females as well as a preparation method and an application thereof, relating to the technical field of extracting effective substances from the pine pollen. The preparation method comprises the following steps: the pine pollen is subjected to wall breaking at a low temperature, and is extracted with ultrasonic after the pine pollen is immersed in normal-pressure normal-temperature water; solid impurities are removed to select water-soluble substances in the pine pollen; and effective components with the molecular weight lower than 5,000D is ultra filtered and separated at the environmental temperature of between 1 and 5 DEG C to obtain the gymnosperm pine pollen aqueous extract for treating the premature ovarian failure. The pine pollen aqueous extract can be used for preparing an oral preparation for treating the premature ovarian failure, has a good absorption effect and low price; and the pine pollen aqueous extract can be used for preparing an injection preparation for treating the premature ovarian failure, which can be absorbed quickly, has strong treatment pertinence, can be used for general health-care of the females, and prevents occurrence of the premature ovarian failure.

The invention provides an asexual rapid propagation method for dicotyledons. The method comprises the following steps: 1) cutting leaves with complete axillary buds from new branches of dicotyledon plants in the current year; 2) removing xylem tissues at the basal part of the petiole, then putting the leaves into sterile water for cleaning, and taking the trimmed leaves as explants; 3) spraying arooting powder solution to the leaf surfaces of the explants once, and then putting the leaf explants into a seed germination box for water culture until petioles root; 4) transplanting the rooted leaf explant into soil for cultivation. According to the method, the leaf explants are utilized for the first time to establish a method for rapidly propagating and nursing dicotyledon (such as tea trees, peanuts and cotton) materials, and the method is reliable and stable. Dicotyledon plant leaves for propagation are sufficient in source, few materials are needed for experiments, the survival rate of new plants is high, the experiment period is short, and the method has the potential of factory-like large-scale production. The method has great application value in the fields of rapid propagationand popularization of excellent varieties of tea trees, preservation of specific materials and the like. The method also has a certain reference value for gymnospermae and monocotyledons.

In one aspect, the invention provides a method of modulating the development stage of strobili growing on a gymnosperm tree comprising: (a) installing a protective covering over one more strobili bud(s), wherein the type of protective covering is selected to delay or advance strobili development; and (b) maintaining the protective covering over the strobili bud(s) for a time period sufficient to delay or advance the development stage of the covered strobili buds in comparison to the development stage of naturally growing, uncovered, control strobili.

The invention provides a Cunninghamia virus induced gene silencing system and a construction method thereof and belongs to the field of forest gene engineering. The construction method includes the steps of cultivating a Cunninghamia plant material; cloning a target gene fragment; constructing a recombinant viral plasmid vector; infecting the Cunninghamia plant with an Agrobacterium-mediated silencing vector; verifying silencing effect results. The system and its construction method are suitable for studying the functional mechanism of a target gene according to changes in plant phenotype; theCunninghamia VIGS (virus induced gene silencing) system is of pioneer significance to the research on functional genes of forest gymnosperms.

In angiosperm and gymnosperm plants, overexpressing a SAMdc nucleotide sequence can decrease lignin content and, for plants with woody tissue, increase wood density.

The invention relates to a torreya grandis seed after-ripening treatment method for promoting embryo development, used for gymnosperm taxaceae torreya.. Pearlite or river sand is soaked by clear water under the condition that no water is dropped when the pearlite or the river sand is held by hands and a soaked pearlite or river sand layer is paved at the bottom of a container, then torreya grandis seeds are placed in a layer way, and the soaked pearlite or river sand layer is covered on the torreya grandis seeds, and then other seeds are placed again, and then the soaked pearlite or river sand layer is covered on the seeds by parity of reasoning until the upper part of the container is covered by the pearlite or river sand layer; then poikilothermic treatment is carried out for 90-100 days; inspection is carried out and the pearlite or river sand is maintained to be moist, and no water is accumulated; after the seeds are subjected to after-ripening treatment for 90 days by adopting the method, not only reaches embryo development rate up to more than 95%, and dissection shows that torreya grandis grow to a fresh green mature embryo with the length of being about 2cm from a flavescent young embryo with the length of being about 0.001cm and the size same as that of a needle point, and the mature embryo almost penetrates trough the whole torreya grandis seed; and secondly, the torreya grandis seed after-ripening treatment method disclosed by the invention is applicable to sowing, raising seedling and tissue culture of the mature embryo, cotyledons, embryo, embryonic axis and endosperm of the torreya grandis and is also applicable to all torreya plants in the world.

The invention provides a pine pollen extract for treating fatty liver as well as a preparation method and an application thereof, relating to the technical field of extracting effective substances from gymnosperm pine pollen. The preparation method comprises the following steps: the pine pollen is subjected to wall breaking at a low temperature, and impurities in the pine pollen are removed; the pine pollen is extracted with ultrasonic after the pine pollen is immersed in normal-pressure normal-temperature water; solid impurities are centrifugally removed to obtain an aqueous solution; and a gymnosperm pine pollen aqueous extract with the molecular weight lower than 3,000D is separated at the environmental temperature of between 1 and 5 DEG C to obtain the gymnosperm pine pollen aqueous extract for treating the fatty liver. The pine pollen aqueous extract can be used for general health-care for children, and can prevent occurrence of fatty liver. The pine pollen aqueous extract can beused for preparing an oral preparation for treating the fatty liver, has a good absorption effect and low price, and also can be used for preparing an injection preparation for treating the fatty liver, which can be absorbed quickly, has strong treatment pertinence.

The invention specifically provides a mechanical device using electrical stimulation to separate earthworms from cultivation soil thereof, solving the current problem that the efficiency of separating earthworms from cultivation soil thereof is low, living earthworms are easy to be damaged and cleaning operation is relatively hard to perform. A feeding assembly comprises separation column frames. Clearance support rods are arranged among adjoining separation column frames. The bottom end of the lowermost layer of the separation column frames is provided with a loading material plate. An electric expelling assembly comprises a fixing column. A cone-shaped electric expelling hollow part is arranged on the fixing column. The outer side of the cone-shaped electric expelling hollow part is provided with an embossing-shaped structure of gymnosperm cycas root surface. An earthworm separation mechanism comprises a hollow rotating shaft. The top end of the hollow rotating shaft is connected with a disc-shaped material dropping plate. The disc-shaped material dropping plate is provided with a dumping opening one. The bottom end of the hollow rotating shaft is provided with a motor. The output end of an electronic control system is connected with the input ends of the motor and the cone-shaped electric expelling hollow part. A dumping plate is aslant arranged in a cabinet. The mechanical device achieves the purpose of quickly separating earthworms from cultivation soil thereof.

The invention discloses gymnosperm isolated culture method and the special using culture medium. The culture medium receipt is that: cane sugar 120-150g, boric acid 0.1-0.2g and calcium chloride 0.1-0.3g. They are settled to 1000 ml by water. The culturing method is that the gymnosperm pollen is hydrated for 20-30 minutes under room temperature, then it is cultured in the said medium for 6-72 hours, finally the pollen tube is got after pollen sprouting. The essential environment is supplied for pollen sprouting and growth. The culture by this invention is not only fast and easy, but also good in culturing result. A great deal of gymnosperm pollen tube with vitality can be got, and there is great practical application value.

The invention discloses a labeling method of an apple pollen tube microfilament. The labeling method comprises the following steps: cultivating apple pollen, curing the apple pollen, performing enzyme treatment on the apple pollen, treating the apple pollen with a surfactant, performing dye labeling, and performing scanning and imaging. The labeling method has the beneficial effect that the labeling method of gymnosperm pollen tube microfilament is referred and improved on the basis of the labeling method of pear pollen tube microfilament, dyes enter cells easier through curing, enzymolysis of cell walls and the surfactant, the interference of cell outer wall autofluorescence due to insufficient enzymolysis as TRITC-Phalloidin is applied are avoided when FITC-Phalloidin labeling is applied, so that relatively complete apple pollen tube microfilament can be stored; moreover, the labeling method is efficient and quick, can be used for obtaining relatively clear and bright pictures, thus filling up the blank of the apple pollen tube microfilament labeling, providing reference for labeling microfilament in rosaceae and angiosperm pollen tube, and achieving a high application value.

A method for preparing the pollen without external wall of gymnosperm includes such steps as enzymolyzing the pollen of gymno sperm in the enzymolyzing liquid containing cellulase (1-2 mass%) and macerozyme (0.5-1.5) for 1.5-2 hr, and slightly pressing the enzymolyzed pollen particles.

The present invention discloses a method for detecting gymnosperm pollen tube interior antigen. Said method includes the following steps: (1), using phosphoric acid buffer solution containing paraformaldehyde to fix pollen tube, using zymolysis solution containing cellulose and macerozyme to zymolyze gymnosperm pollen tube, using Triton X-100 or NP-40 aqueous solution whose mass percentage concentration is 0.2-1% to wash the pollen tube; and (2), making the pollen tube treated by step 1 and the correspondent antibody for resisting the antigen to be tested undergo the process of immunofluorescence reaction, if the fluorescence is detected, the gymnosperm pollen tube interior contains antigen to be tested and can obtain the location condition of said antigen to be tested.