55 results about "Translational efficiency" patented technology

Novel cap analogs which are easily synthesized, resulting in high levels of capping efficiency and transcription and improved translation efficiencies are provided. Such caps are methylated at the N7 position of one or both guanosines of the dinucleotide cap as well as at the 3′ position on the ribose ring. Substituent groups on the ribose ring also result in the cap being incorporated in the forward orientation. Also provided are methods useful for preparing capped analogs and using mRNA species containing such analogs are also contemplated herein, as well as kits containing the novel cap analogs.

It was the object of the present invention to provide RNA with increased stability and translation efficiency and means for obtaining such RNA. It should be possible to obtain increased grades of expression by using said RNA in gene therapy approaches.

The present invention discloses construct systems and methods for comparing different iso-accepting codons according to their preference for translating RNA transcripts into proteins in cell or tissues of interest or for producing a selected phenotype in an organism of interest or part thereof. The codon preference comparisons thus obtained are particularly useful for modifying the translational efficiency of protein-encoding polynucleotides in cells or tissues of interest or for modulating the quality of a selected phenotype conferred by a phenotype-associated polypeptide upon an organism of interest or part thereof.

A method of constructing a synthetic polynucleotide, the method including selecting a first codon of a parent polynucleotide that encodes a polypeptide for replacement with a synonymous codon, wherein the synonymous codon is selected on the basis that it exhibits a higher translational efficiency in an epithelial cell of a mammal than the first codon in a comparison of translational efficiencies of codons in test cells of the same type as the epithelial cell; and replacing the first codon with the synonymous codon to construct the synthetic polynucleotide.

The embodiment of the invention discloses a scanning translation method and device, a scanning pen and a related product, and the method comprises the steps: translating a first scanning text scanned at the ith time in a time interval from the end of the ith scanning of the scanning pen to the start of the (i+1) th scanning, and obtaining a first translation intermediate result; transferring and storing the first translation intermediate result; and after the (i+1) th scanning is finished, translating a second scanned text scanned for the (i+1) th time according to the transferred first translation intermediate result, so that the calculated amount in the translation process of the second scanned text can be reduced by translating the second scanned text scanned for the (i+1) th time according to the transferred first translation intermediate result. Repeated calculation does not need to be performed on the first scanning text, waste of calculation resources is avoided, and the translation efficiency of the scanning pen is improved; according to the context information represented by the first translation intermediate result, the accuracy and coherence of the translation result are improved, so that the translation effect of the scanning pen is improved.