189 results about "Autophagy" patented technology

The invention discloses an unmanned aerial vehicle remote sensing quantitative marine ecological restoration effect evaluation system, and relates to the technical field of marine ecological monitoring and evaluation, and the system comprises a rhythm resonance excitation module, a resonance coupling inversion module, an error autophagy verification module, and a resonance steady state adjustment module. According to the invention, a three-in-one framework of a rhythm resonance coupling mechanism, an error autophagy verification mechanism and a rhythm phase latch-cross-scale energy anchoring stator mechanism is utilized, and four core modules of rhythm resonance excitation, resonance coupling inversion, error autophagy verification and resonance steady state adjustment are combined. The method solves the problems of low evaluation precision, no self-verification capability and large errors caused by marine environment interference due to manual sampling recheck and rhythm splitting in the prior art, realizes quantitative evaluation and self-verification of the marine ecological restoration effect without manual sampling, remarkably improves evaluation efficiency, credibility and precision, and is suitable for large-scale popularization and application. And micro-scale power data can be synchronously output, the endurance of the unmanned aerial vehicle is prolonged, and the restoration trend is pre-judged.

Described herein are compounds that are inhibitors of autophagy and their use in the treatment of disorders such as cancers.

Described herein are compounds that are inhibitors of autophagy and their use in the treatment of disorders such as cancers.

A novel autophagy activator is provided. An autophagy activator, a hyaluronic acid production promoter, and an antioxidant, each of which contains a lactic acid fermentation product obtained by fermenting a raw material derived from a tea tree with lactic acid bacteria.

The invention discloses a magnetic-aptamer targeting tumor tissue nano-drug delivery system as well as a preparation method and application thereof, and belongs to the field of medicines. The system comprises a mesoporous ferroferric oxide magnetic core, a polyethyleneimine (PEI) layer coated outside the mesoporous ferroferric oxide magnetic core and an aptamer adsorbed outside the PEI layer, and an autophagy activator can be selectively loaded in the mesoporous core. The preparation method comprises the following steps: sequentially carrying out drug loading, PEI coating and aptamer modification on the mesoporous Fe3O4 nanoparticles. According to the invention, efficient enrichment and endocytosis of tumor tissues are realized through dual effects of magnetic targeting and active targeting of the aptamer; lysosome escape is promoted by using the proton sponge effect of PEI; finally, by virtue of the synergistic effect of iron ions released by degradation of the Fe3O4 nanoparticles and endogenous iron ions released by ferritin autophagy induced by an autophagy activator, ferroptosis of tumor cells is induced through enhanced Fenton-like reaction, so that high-efficiency and low-toxicity targeted therapy is realized.

The invention belongs to the technical field of biological medicine, and relates to application of a compound D25, in particular to application of a small molecule compound D25 in preparation of a product for preventing or treating Alzheimer's disease (AD), and the structural formula of the compound is shown in the specification. Experiments prove that the small molecule compound D25 can pass through a blood brain barrier, can induce autophagy, can relieve pathological characteristics of an AD mouse model and improve cognitive impairment of the AD mouse model, can reduce the proportion of disease-related microglial cells by recovering the morphology of the microglial cells and improving the A beta phagocytosis capacity of the microglial cells, has the potential of promoting neuronal growth and increasing the number of protrusions, and can be used for preparing a medicine for treating the disease-related microglial cells. Therefore, AD can be effectively treated, the safety is high, the effect is achieved, and the application prospect is great.

This invention discloses a multi-target synergistic anti-liver cancer medical food granule and its preparation method. The granule uses medicinal and edible homologous foods and novel resource foods as raw materials, and is composed of curcumin extract, esterified fat-soluble EGCG, Moringa leaf powder, yeast β-glucan, Astragalus extract, Schisandra extract, Salvia miltiorrhiza extract, Poria cocos powder, resistant dextrin, and erythritol in a specific ratio to construct a three-dimensional synergistic system of "direct killing-immune regulation-liver protection," which can induce ferroptosis in liver cancer cells, inhibit protective autophagy, activate the body's immunity, and protect liver cells. This invention employs directional extraction, multi-layer coating, and high-pressure fusion granulation technology. Nanoparticle liposome encapsulation, esterification modification, and pH-responsive enteric coating improve the stability and bioavailability of the components, solving the problems of poor water solubility, low absorption, and easy degradation of natural active ingredients. The granule has clear efficacy, high safety, and stable process, and can be used as a medical food for adjuvant therapy and nutritional support during the recovery period of liver cancer.

A series of 1, 3, 5-triazine compounds that exhibit potent inhibition of endosomal trafficking and autophagy is provided. These compounds effectively suppress cancer cell proliferation, growth, and migration, induce cell cycle arrest at the G0 / G1 phase, promote cancer cell death via non-apoptotic pathways, and inhibit tumor sphere formation. Moreover, select compounds within this series demonstrate significant inhibition of tumor growth and metastasis in mouse models of lung cancer and melanoma. Additionally, they modulate macrophage polarization and the tumor microenvironment by regulating cytokine secretion. These findings highlight the potential of 1, 3, 5-triazine compounds as promising antitumor agents.

The invention discloses an oral hard capsule health-care product with an anti-aging function as well as a preparation method and application of the oral hard capsule health-care product. The health-care product is mainly prepared from eight active ingredients including EGT (Ergothioneine), Ca-AKG (alpha-ketoglutarate), PQQ (pyrroloquinoline quinone), fisetin, salidroside, urolithin A, spermidine and an apple stem cell extract. Through scientific compatibility of the various components, the composition disclosed by the invention can comprehensively intervene in a cell aging process through a synergistic effect of a plurality of pathways of enhancing the cell antioxidant capacity, improving the mitochondrial function, activating cell autophagy, promoting cell regeneration and repair and the like. The invention also provides a preferred content range of the health-care product, a preparation method of the health-care product and application of the health-care product in preparation of health-care foods for delaying senescence, improving cell viability or improving mitochondrial functions. The product provided by the invention has the characteristics of multiple targets and synergistic interaction, and provides a new effective scheme for anti-aging intervention.

The invention discloses an application of ametinib in preparation of a medicine for treating liver cancer, belongs to the technical field of new application of medicines, and verifies the potential of third generation EGFR-TKI ametinib in inhibiting liver cancer progression in vivo and in vitro, and cell function experiments prove that ametinib can effectively inhibit proliferation, migration and invasion ability of liver cancer cells; the exact type of ametinib for inducing liver cancer cell death is determined, and ametinib inhibits survival and proliferation of liver cancer cells by activating autophagy and inducing ferroptosis; the application proves that autophagy activated by the ametinib can promote the occurrence of ferroptosis by regulating and controlling the degradation of GPX4 (glutathione peroxidase 4) and the accumulation of reactive oxygen species (ROS), and the ametinib inhibits the progress of liver cancer by inducing autophagy-dependent ferroptosis, so that the ametinib is used for preparing the medicine for treating liver cancer.

The invention relates to the fields of pharmacology and molecular biology, and discloses an autophagy inhibitory polypeptide competitively combined with an LIR docking site of an autophagy-related protein LC3 and application of the autophagy inhibitory polypeptide. Through molecular docking simulation, through LIR docking sites of targeted LC3 protein, 794 bioactive polypeptides which can be combined are screened out. Functional result analysis shows that peptide 3 #, peptide 4 # and peptide 5 # are competitively combined with LIR docking sites of the LC3 protein, so that formation of autophagy vesicles is inhibited in a targeted manner, and proliferation and survival of autophagy-dependent pancreatic cancer cells are effectively inhibited; the strategy is combined with chemotherapeutic drugs to show a synergistic effect, and the pancreatic cancer resisting curative effect is remarkably enhanced.

This invention relates to the field of cosmetic compositions, and more specifically to a synergistic anti-aging composition involving energy metabolism and autophagy, its preparation method, and its application. The composition comprises sea buckthorn fruit extract, trehalose, and a cosmetically acceptable polyol carrier. This invention pioneers a betaine-trehalose-NADES extraction system, achieving low-temperature, high-efficiency extraction while preserving the heat-sensitive active ingredients of sea buckthorn. Trehalose possesses both extraction and autophagy activation effects. A selective separation strategy achieves a balance between efficacy, compliance, and process simplicity. The green, circular process reduces costs and is environmentally friendly. Through the synergistic effect of sea buckthorn fruit extract and trehalose, the composition enhances mitochondrial membrane potential, increases ATP production, inhibits excessive activation of the mTOR pathway, and promotes LC3-II conversion, thus synergistically combating skin aging from multiple targets including cellular energy metabolism, signaling pathways, and autophagy.

The present disclosure relates, in certain embodiments, to the finding that certain compounds that modulate the activity(ies) of Sigma receptors can be used to disrupt virus lifecycle, infection, and / or dissemination. The compounds contemplated in the disclosure are useful in the prevention, treatment, and / or amelioration of virus infection, either alone or in combination with at least one additional therapeutic agent, which can be an antiviral agent and / or an agent that treats, ameliorates, and / or prevents one or more virus infection symptoms and / or co-morbidities. In certain embodiments, the Sigma receptor is a Sigma-1 receptor (also known as Sigma1) or Sigma-2 receptor (also known as Sigma2 or TMEM97). In certain embodiments, Sigma1 inhibitors / antagonists that cause and / or trigger ER stress and / or autophagy are useful within the methods of the disclosure.

The invention relates to the technical field of poultry breeding, and particularly provides a novel application of miR-17-5p in chicken follicular development. Experiments prove that the miR-17-5p can inhibit autophagy and apoptosis of chicken follicular granular cells, so that granular cell proliferation can be promoted by promoting expression of the miR-17-5p in the chicken follicular granular cells, development of chicken ovary follicles is further promoted, and atresia is prevented. The invention also discovers that downstream target mRNA of miR-17-5p is MAP3K5, and researches find that MAP3K5 can promote autophagy and apoptosis of chicken follicular granular cells. The discovery provides a new thought for targeting particle cells to regulate dynamic balance between autophagy and apoptosis of the cells so as to improve the poultry breeding efficiency, and also expands the functional cognition of the JNK / BCL2 pathway in poultry research.

The invention relates to a truffle composition as well as a preparation method and application thereof, and belongs to the technical field of daily chemical products. The invention provides a truffle composition which comprises a truffle extract, retinol and nonapeptide-1, and the mass ratio of the truffle extract to the retinol to the nonapeptide-1 is as follows: the truffle extract to the retinol to the nonapeptide-1 is (0.026-200): (0.005-4): 1. The truffle extract disclosed by the invention has the effects of resisting inflammation, resisting oxidation, tightening and resisting wrinkles, and autophagy and brightening, and the retinol has the effects of tightening and resisting wrinkles; the nonapeptide-1 serving as a rhythm peptide has a rhythm rejuvenation effect, and the three components are matched to form the truffle composition which can synergistically improve a retinol metabolic pathway, accurately regulate and control retinol metabolism to remove oxidative damage, regulate and control anti-aging genes and collagen genes of skin and synergistically realize an anti-aging effect.

The invention relates to a brightening composition for improving skin transparency and glossiness and application of the brightening composition, and belongs to the technical field of cosmetics. The brightening composition comprises oxyresveratrol, a loquat leaf extract, a burdock seed extract and a verbascos extract, the composition can effectively resist oxidation and inflammation through the oxyresveratrol so as to relieve skin darkness, and the loquat leaf extract can promote cell autophagy so as to remove metabolic waste and improve skin permeability. The burdock seed extract improves the epidermis structure so as to enhance the skin light transmission, and the verbascos extract plays a role in instant brightening through a quantum transition effect. According to the composition, through the synergistic effect of all the components, the skin transparency and glossiness are remarkably improved, and the composition is mild, free of irritation and suitable for being used by people with sensitive skin.

The invention relates to an effect of an REV-ERBalpha agonist SR9009 in the aspect of delaying ovarian senescence, and aims at the problem of fertility decline caused by cliff-type decline of ovarian functions of women over 35 years old, the SR9009 is applied to activate an REV-ERBalpha circadian rhythm signal channel in ovarian tissues, inhibit excessive autophagy of granular cells and maintain a primordial follicle dormancy state, so that the agonist SR9009 can be used for delaying ovarian senescence. Therefore, the original follicle depletion speed is delayed, follicle storage is maintained, and the ovary reproductive function cycle is prolonged. The effect is verified by a mammal model: REV-ERBalpha protein expression in an ovary of an aging individual is reduced, rhythmicity expression is disordered, autophagy of granulosa cells can be effectively inhibited in the ovary of the aging individual intervened by the SR9009, and the number of residual primordial follicles and the number of total follicles are obviously greater than those of an untreated control group. The invention provides an innovative treatment strategy of targeted rhythm regulation and control for clinical intervention of ovarian aging.

The invention discloses double-response hydrogel as well as a preparation method and application thereof. The double-response hydrogel comprises a three-dimensional porous network hydrogel matrix, and tea polyphenol and nano silicon nitride which are loaded in the hydrogel matrix, the three-dimensional porous network hydrogel matrix is prepared from o-nitrobenzene grafted hyaluronic acid and carboxyl phenylboronic acid modified methyl chitosan. According to the hydrogel disclosed by the invention, in a low-pH / high-ROS microenvironment of a diabetic periodontitis focus, boric acid ester bonds are broken, tea polyphenol is released, and the tea polyphenol plays an anti-inflammatory role by efficiently removing active oxygen and inhibiting inflammatory factors; meanwhile, the Schiff base bond is dissociated to release the nano silicon nitride, and the nano silicon nitride significantly promotes angiogenesis and osteogenic differentiation and accelerates bone defect repair by activating a redox signal, inducing cell autophagy, regulating metabolic reprogramming and other pathways.

The invention belongs to the field of biological medicine, and relates to application of triptolide in preparation of a medicine for preventing and treating calcified aortic valve diseases. The triptolide is found to be capable of promoting expression and maturation of CTSD and activating enzyme activity of the CTSD, meanwhile, biogenesis of lysosome in valvular interstitial cells can be promoted by regulating and controlling AKT / mTOR / TFEB signal axes, lysosome functions are improved, autophagy flux is recovered, and finally the effect of treating the calcified aortic valve disease is achieved. A novel treatment medicine is provided for the calcified aortic valve disease.

Provided is a method of screening for an autophagy modulator. According to the screening method of an aspect, the autophagy modulator can be accurately screened with only a single cell. In addition, screening can be performed without using fluorescent labeling, and thus cytotoxicity and photobleaching that occur when fluorescent labeling analysis methods are used can be prevented, and the limitation of having to monitor only fluorescently labeled markers can be improved.

The present application relates to the technical field of biological medicine, and more particularly to a culture solution, a kit and an application for in-vitro maturation of aged oocytes. Through in-vitro experiments, the present application adds exogenous stromal cell-derived factor 1, establishes a culture system for in-vitro maturation of aged oocytes, and reveals the molecular mechanism of stromal cell-derived factor 1 playing a role through autophagy, thereby providing a new solution for improving the fertility of aged women and improving the success rate of assisted reproductive technology, and having important clinical application value.

In various embodiments compounds that induce and / or promote autophagy are provided as wells as methods of use thereof (e.g., in the prophylaxis or treatment of chronic pathologies associated with aging). In one illustrative, but non-limiting embodiments the compound(s) comprise, inter alia, coumarin 106.

This invention discloses a cell preparation targeting bone metastatic tumor cells, its preparation method, and its application, belonging to the field of biomedical technology. The cell preparation comprises senescent neutrophils and drug-loaded cationic liposomes internalized within them. The cationic liposomes encapsulate a STING agonist and a pan-photokinase inhibitor. The preparation method includes: preparing drug-loaded liposomes using a thin-film dispersion-ultrasound method; obtaining senescent neutrophils through in vitro culture of extracted neutrophils; and preparing a senescent neutrophil preparation loaded with liposomes. This invention utilizes the natural bone marrow homing ability of senescent neutrophils to achieve precise targeting of bone metastatic tumor cells, and co-delivers two drugs through liposomes, simultaneously activating anti-tumor immunity and inducing tumor cell apoptosis / autophagy, synergistically treating bone metastases. This system has high drug loading capacity, good encapsulation efficiency, and sustained-release effect, and the preparation process is simple and controllable, providing a new strategy for the treatment of cancer bone metastases.

The application belongs to the technical field of biological medicine, and particularly relates to application of an active component of chamomile in preparation of an asthma treatment preparation. The chamomile active component is prepared by the following method: chamomile is subjected to reflux extraction with ethanol as an extraction solvent to obtain an extract; the extract is purified, eluted with water and ethanol in sequence, and the eluate of ethanol is collected and dried to obtain the chamomile active component. The chamomile active component provided in the application can regulate autophagy and apoptosis through multiple targets, inhibit airway inflammation, and improve immune imbalance, and can not only effectively relieve asthma symptoms, but also reduce airway epithelial damage and inhibit airway remodeling, thereby overcoming the defects of existing drugs, such as large side effects, limited efficacy on severe and hormone-resistant asthma patients, and difficulty in reversing airway damage. The active component is derived from a natural plant, is safe, and has a good application prospect in preparation of asthma treatment drugs.

This alternative autophagy activator and this autophagy activator contain at least one selected from the group consisting of dehydrocafestol (DC), dehydrokahweol (DK), cafestol (C), and kahweol (K).

This invention belongs to the field of antitumor drug delivery technology, and discloses a dual-drug sequential delivery system, a composite hydrogel, its preparation method, and its applications. This invention achieves sequential release by encapsulating a cobalt (Co)-polyphenol coordination nanozyme (CoNZ) in a hydrogel and integrating it with microfibrils loaded with chloroquine (CQ). This invention provides a promising new approach to improving the treatment of CRPC by initially inducing ROS-mediated oxidative damage, subsequently inhibiting autophagy and blocking the TLR9 / NF-κB signaling pathway, synergistically disrupting the survival mechanisms of tumor cells.

The invention belongs to the technical field of pharmacy, and particularly relates to application of spermidine in sepsis treatment. Experiments prove that spermidine can regulate mitochondrial biogenesis and inhibit mitochondrial excessive autophagy to remarkably improve mitochondrial quality, maintain mitochondrial homeostasis and relieve myocardial dysfunction and heart structure damage. In addition, spermidine can inhibit oxidative stress caused by excessive inflammatory response so as to recover normal functions of mitochondria. The spermidine has a potential treatment effect on sepsis and has a good clinical application prospect in treating sepsis and related diseases caused by excessive autophagy.

The invention belongs to the technical field of biomedical detection, and particularly discloses a nerve injury early warning method and system for abnormal metabolism of iron ions, and the method comprises the steps: synchronously obtaining the steady-state real-time dynamic data of the iron ions and the multi-dimensional biomarker data of the autophagy activity of ferritin; a data basis for comprehensively reflecting the iron metabolism state is constructed; the complex coupling relation between the iron ion steady state and the ferritin autophagy is deeply analyzed through an iron metabolism abnormality coupling analysis model, a nonlinear pathological mode under the synergistic effect of the iron ion steady state and the ferritin autophagy is disclosed, and the accuracy and reliability of early warning decision making are improved; by analyzing the specific mode of iron ion metabolism abnormal state coding, identifying key driving factors causing risks, combining with the early warning level of nerve injury and matching with an individualized intervention scheme, closed-loop design from early warning to accurate intervention is realized, operable guidance is provided for clinical practice, and the method is suitable for popularization and application. And conversion from passive treatment to active defense is realized.

The invention discloses a tumor personalized treatment method based on AI analysis of molecular chaperone autophagy characteristics, and relates to the technical field of medical artificial intelligence and tumor treatment. The method at least comprises the following steps: S1, data acquisition and preprocessing: collecting multi-modal data, labeling the multi-modal data, and increasing diversity through an image enhancement technology, the image enhancement technology at least comprises rotation, zooming and color disturbance, and data standardization is carried out; according to the invention, through multi-modal data fusion, the comprehensiveness and accuracy of CMA feature analysis are improved; the LSTM is used for processing dynamic data, the time sequence change of the CMA is captured, and the practicability of the model in a clinical environment is enhanced; transfer learning and image enhancement are adopted, so that the dependence on large-scale annotation data is reduced, and the model robustness is improved; finally, individuation of tumor treatment is achieved, side effects are reduced, and the curative effect is improved.

To provide protective agents with excellent effects such as starch retrogradation inhibition, prevention or inhibition of the decrease in activity of proteins during storage in a frozen or freeze-dried state, prevention or inhibition of damage or death of microorganisms during storage in a frozen or freeze-dried state, and pH buffering; surfactants with excellent surfactant properties; blood glucose control compositions that do not raise blood glucose levels when administered to individuals; autophagy inducers with excellent autophagy induction properties; staining agents for acid-fast bacteria that can easily and efficiently stain acid-fast bacteria; and protein extractants with excellent protein extraction properties. [Solution] A protective agent, surfactant composition, blood glucose control composition, autophagy inducer, acid-fast bacilli staining agent, or protein extractant characterized by containing a compound represented by general formula (1).