545 results about "Autophagy" patented technology

The invention relates to a barrier repair composition for activating cell energy and application thereof, the composition comprises the following components: nicotinamide, a wheat germ extract, oat beta-glucan and a golden camellia flower extract, the four components synergistically form a full-link barrier repair system of energy supply (nicotinamide)-autophagy cleaning (wheat germ extract)-structure strengthening (beta-glucan)-oxidation defense (camellia nitidissima extract), the limitation that a traditional skin care product singly supplements lipid or resists oxidation is broken through, lipid dynamic balance is rebuilt from the root of cell metabolism, and the skin care product has the advantages that the skin care effect is good; and certain skin smoothing and anti-aging effects are achieved.

The invention discloses a PROTACs compound for targeted degradation of PRMT5 and application of the PROTACs compound. The PROTACs compound comprises a compound as shown in the following formula, and related analogues or pharmaceutically acceptable salts, metabolites or prodrugs of the compound: in the # imgabs0 # formula, X represents a ligand bound to PRMT5 protein, Z represents a ligand of E3 ubiquitin ligase, and Y represents a linking group for linking X and Z; according to the invention, CRBN is used as E3 ligase, and a series of PRMT5-targeted PROTAC degradation compounds are designed and synthesized. The compound can effectively induce PRMT5 degradation, significantly inhibit proliferation, growth, migration, infiltration, clone formation and metastasis of tumor cells, promote apoptosis of cancer cells, promote autophagy of the tumor cells, prevent or overcome drug resistance of BTK inhibitors and / or prolong the lifetime of tumor patients, and has wide application prospects.

The present invention provides a Lactiplantibacillus plantarum ( Lactiplantibacillus plantarum ), strain 24, which is deposited in the China General Microbiological Culture Collection Center, deposit number: CGMCC No. 31278. The Lactiplantibacillus plantarum 24 of the present invention can secrete sphingomyelinase to promote the production of ceramide in HaCaT cells; Lactiplantibacillus plantarum 24 or its metabolite or its lysate can up-regulate the expression of key enzymes in the sphingomyelinase and / or ceramide synthesis pathway of skin cells, repair the skin barrier; down-regulate the expression of pro-inflammatory cytokines, inhibit autophagy, and has a good anti-inflammatory effect; at the same time, it also has good antioxidant and whitening effects. The Lactiplantibacillus plantarum 24 or its metabolite or its cell lysate of the present invention can be used in the preparation of products having the above-mentioned effects or functions.

The invention provides a lactobacillus plantarum 24 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the lactobacillus plantarum 24 is CGMCC No.31278. The lactobacillus plantarum 24 has the advantages that the lactobacillus plantarum 24 is prepared from lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum and lactobacillus plantarum; the plant lactobacillus 24 disclosed by the invention can secrete sphingomyelinase and promote HaCaT cells to generate ceramide; the plant lactobacillus 24 or the metabolite or lysate thereof can up-regulate expression of key enzymes in a synthetic route of skin cell sphingomyelinase and / or ceramide, and repair a skin barrier; expression of proinflammatory cytokines is lowered, autophagy is inhibited, and a good anti-inflammatory effect is achieved; meanwhile, the anti-oxidation and whitening effects are relatively good. The plant lactobacillus 24 or the metabolite or the cell lysate of the plant lactobacillus 24 can be applied to preparation of products with the effects or the effects of the plant lactobacillus 24 and the metabolite or the cell lysate of the plant lactobacillus 24.

The invention relates to application of a tea exosome in preparation of a medicine for improving skin pigmentation, the tea exosome is creatively extracted and purified, and the tea exosome can effectively inhibit proliferation of melanoma cells and inhibit expression of melanin generation related protein by scavenging free radicals and inhibiting tyrosinase activity, so that the effect of improving skin pigmentation is achieved. The intracellular melanin removal is promoted. In a mouse in-vivo experiment, after the tea exosome is applied, it is observed that the skin epidermis thickness of a mouse is reduced, the dermis thickness is increased, collagen arrangement is tighter and more ordered, the content is increased, and meanwhile generation of inflammatory factors is reduced. The tea exosome can significantly increase the content of a melanin autophagy key factor LC3B and reduce the content of P62 to promote melanin autophagy, and has good percutaneous infiltration capacity, thereby playing a role in improving skin pigmentation.

Disclosed are methods, compounds, and compositions useful for increasing autophagy and promoting longevity. The methods, compounds, and compositions relate to urolithins and urolithin precursors and use thereof. Certain urolithins are represented by Formula I, while certain urolithin precursors are represented by Formula IV. The urolithin may be urolithin A, urolithin B, urolithin C, or urolithin D. The urolithin precursor may be ellagic acid or an ellagitannin. The methods include in vivo, ex vivo, and in vitro uses of the compounds and compositions.

The invention discloses an osthole compound, a metal complex and a preparation method and application of the osthole compound, the osthole compound (OST-Alkyne) has remarkable anti-tumor biological activity, osthole (OST) and a cyclometalated iridium precursor are further connected through a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction, higher anti-tumor activity is shown, and the osthole compound has a good application prospect. Mitochondria is targeted, and tumor is killed by reducing mitochondrial membrane potential and inducing cell autophagy and necrotic apoptosis; according to the invention, natural products are combined with metals, so that not only are good selectivity and anti-proliferation ability to brain glioma cells realized, but also toxic and side effects to normal cells are overcome, a new design idea is provided for novel metal drugs to treat brain glioma, and the application prospect is wide.

A method of potentiating musculoskeletal effect of one or more anabolic amino acids in an individual in need thereof can include administering to the individual in need thereof a composition containing the one or more anabolic amino acids, the composition also containing one or more autophagy-inducing amino acids in a total amount effective for the composition to be at least neutral regarding autophagy. In another aspect, a method of overcoming one or more negative effects of one or more anabolic amino acids by preventing degenerative processes related to loss of autophagy can include administering to an individual in need thereof a composition containing the one or more anabolic amino acids, the composition further containing the one or more autophagy-inducing amino acids in a total amount effective for the composition to be at least neutral regarding autophagy.

The invention belongs to the technical field of cell biology, and particularly relates to a method for detecting cell stability, a cell sample is detected in three aspects of cell performance, cell mutation and cell stability, and when the cell proliferation effect is good, the cell does not migrate, and the cell activity is gt; the cell of which the cell concentration is 80%, the cell early-stage and late-stage apoptosis ratio is less than or equal to 10%, the cell autophagy ratio is less than 5%, no proto-oncogene is remarkably expressed, the cell aging ratio is less than or equal to 20% and the cell multiplication time is less than 48 hours is a stable cell; if one of the indexes does not reach the standard, it is determined that cell passage is unstable, and the cell culture process is stopped. The method provided by the invention can comprehensively judge the life cycle of the cell, so that the cell is applied to the optimal economic / efficient service life, the cell state can be effectively and accurately judged, and the method has good reliability.

The invention provides a learning concentration training system based on brain wave analysis, and relates to the field of electroencephalogram signal processing. Comprising the following steps: calculating a user concentration degree and a theta / beta wave energy ratio in real time through an electroencephalogram signal acquisition module, and triggering subsequent processing when the ratio exceeds a threshold value; the quantum coding module converts the electroencephalogram signal into a discrete quantum state carrying a recursive coding chain, and feeds back the discrete quantum state to the reverse interference engine module to generate a chaotic waveform, and the chaotic waveform is reversely injected to form closed-loop interference; the electrode autophagy module dynamically generates electrode topological parameters based on a theta / beta wave steady state activation autophagy protocol and feeds back the electrode topological parameters through a hypergraph network; the kink operation module maps the electroencephalogram energy into a three-dimensional kink during concentration breakthrough and cuts off a low-energy branch, and released energy is converted into an autophagy triggering probability; the compatibility of a recursive chain and historical data is verified through a paradox constraint and a clock synchronization module, a negative probability flow is adjusted to a chaos stability domain, and multi-module synchronization is achieved through a non-von Noremann clock tree.

Described herein are compounds that are inhibitors of autophagy and their use in the treatment of disorders such as cancers.

The invention belongs to the technical field of medicinal chemistry, and discloses a preparation method and application of a benzofuran [2, 3-b] pyridine derivative of a targeted nuclear receptor co-activator 4 (NCOA4). The derivative has a structural general formula as shown in the following formula (I), wherein R1, R2 and R3 substituent groups can be combined at will. The compounds are combined with NCOA4 to interfere the cell iron autophagy process and effectively inhibit the ferroptosis of various cells induced by RSL3 or Erastin. Particularly, the binding affinity of the compound 4k and NCOA4 is optimal (Kd is equal to 0.78 mu M), and the compound 4k has remarkable ferroptosis inhibition activity and can be used for research and development of medicines for ferroptosis-related diseases. # imgabs0 #

Disclosed are methods, compounds, and compositions useful for increasing autophagy and promoting longevity. The methods, compounds, and compositions relate to urolithins and urolithin precursors and use thereof. Certain urolithins are represented by Formula I, while certain urolithin precursors are represented by Formula IV. The urolithin may be urolithin A, urolithin B, urolithin C, or urolithin D. The urolithin precursor may be ellagic acid or an ellagitannin. The methods include in vivo, ex vivo, and in vitro uses of the compounds and compositions.

This disclosure describes the use of azithromycin, roxithromycin, and telithromycin, including derivatives thereof, as senolytic drugs. BrdU was used to induce senescence in model human fibroblast cell lines. Also disclosed are methods for screening compounds for senolytic activity. The SRB assay was used to measure cell viability through protein content. Azithromycin roxithromycin, and telithromycin, clinically-approved pharmaceuticals, were found to be senolytic drugs. However, the closely-related parent compound, erythromycin, showed no senolytic activity. Azithromycin strongly induced both aerobic glycolysis and autophagy in human fibroblasts, but showed bi-phasic effects including on mitochondrial oxygen consumption rates with inhibitory activity at 50 μM and stimulatory activity at 100 μM. The xCELLigence real-time assay system showed that azithromycin preferentially targets senescent cells, removing approximately 97% (nearly a 25-fold reduction in senescent cells).

The invention relates to the technical field of plant genetic engineering, in particular to application of an autophagy related gene VaATG6 in regulation and control of grape cold resistance. The VaATG6 gene disclosed by the invention has a remarkable effect on regulating and controlling the low-temperature stress resistance of grapes. By overexpressing the VaATG6 gene in the grape, the autophagy activity is improved, and the anti-freezing capability of the grape is remarkably enhanced; and the autophagy activity is reduced by knocking out the gene, so that the grapes are more sensitive to low-temperature stress. The invention confirms that the VaATG6 gene is a positive regulatory factor for the cold resistance of grapes, provides an important molecular tool for cultivating a new variety of cold-resistant grapes, and has a wide agricultural application prospect.

This autophagy activator contains at least one of compounds represented by any one of structural formulas (1)-(6).

The invention discloses a recombinant VII type collagen as well as a preparation method and application thereof. The recombinant VII type collagen is obtained by repeating a short amino acid sequence GLPGAKGEKGAP for a plurality of times. The secretion yield of the recombinant VII type collagen in a commercial pichia pastoris expression system can be up to about 17.3 g / L, the extracellular secreted protein is basically not degraded in the fermentation process, the purity can reach 90% or above through simple ultrafiltration and decoloration, and industrial mass production can be achieved; and the recombinant VII type collagen has better biological activities of promoting autophagy, improving an endoplasmic reticulum stress state, promoting collagen secretion and inhibiting matrix metalloproteinase from degrading collagen, and shows good tightening and anti-wrinkle effects.

The invention discloses a method for creating cadmium-resistant rice with low grain cadmium accumulation and application. The rice oshma3oslct1 double mutant expressing the Cd binding autophagy adapter protein AIM-SpMTL-GFP is constructed through CRISPR-Cas9, so that the transfer of Cd from xylem to phloem and the accumulation of Cd in root vacuoles are reduced, meanwhile, the Cd binding autophagy adapter protein AIM-SpMTL-GFP can also accumulate cadmium in protoplast vacuoles of leaves, the yield and quality of rice grains are not influenced, and the yield and quality of the rice grains are greatly improved. Cd accumulation of grains is reduced, and Cd is enriched into straws at non-edible parts, so that industrial treatment is facilitated. The cadmium-resistant rice with low grain cadmium accumulation can reduce cadmium pollution in a food chain, so that the harm of cadmium to a human body is reduced.

The invention discloses an adipose-derived stem cell exosome compound loaded with silver nanoparticles and application of the adipose-derived stem cell exosome compound loaded with the silver nanoparticles, and ADSC-Exos loaded with the silver nanoparticles is developed by integrating AgNPs into an exosome of ADSCs. In vivo, the Exo-AgNPs can accelerate healing of infected wounds by reducing bacterial colonization and promoting collagen deposition, angiogenesis and M2 type macrophage polarization. In vitro, the Exo-AgNPs activates the functions of fibroblasts and vascular endothelial cells, and shows an excellent antibacterial property. In addition, the Exo-AgNPs induces polarization of M2 type macrophages and inhibits secretion of proinflammatory cytokines by activating autophagy. The results show that Exo-AgNPs is expected to become an ideal biological material for treating infected wounds.

The invention relates to the technical field of biological medicine. The invention aims to provide an application of Mn3O4 nanoparticles in relieving intervertebral disc degeneration. The manganous-manganic oxide nanoparticles Mn3O4NPs are added into an interventional drug for treating intervertebral disc degeneration as a nano-enzyme. According to the invention, Mn3O4 nanoparticles are used as a nano active catalytic enzyme, have multienzyme catalytic activity similar to superoxide dismutase, catalase and glutathione peroxidase, can effectively remove reactive oxygen species (ROS) and regulate ECM metabolism, significantly reduce cell apoptosis, delay cell senescence, inhibit inflammation and enhance autophagy, and can be used for preparing a medicine for treating hyperplasia. Therefore, annulus fibrosus AF repair in intervertebral disc degeneration treatment is promoted. The Mn3O4 nano particles have good biocompatibility, metabolites of the Mn3O4 nano particles can be used for other physiological processes, and no toxic or side effect is generated in in-vivo circulation. Meanwhile, the enzyme is stable in low-oxygen and low-pH adverse microenvironment of the intervertebral disc, and has in-vivo stability which is not possessed by other active catalytic enzymes.

A compound represented by chemical formula 1, a salt, a stereoisomer, a solvent or a hydrate thereof, an antibacterial composition including the compound, and uses thereof are disclosed. The antibacterial composition is suitable for inhibiting bacterial infections, preventing, alleviating or treating bacterial infectious disease, and for preventing, alleviating or treating inflammation caused by a bacterial infection. The compound specifically binds to p62 protein so as to activate p62, thereby increasing the activity of autophagy, which is inhibited by a bacterial infection, and thus has antibacterial activity against various bacteria, can inhibit bacterial infectivity and has the excellent effect of enabling prevention, alleviation or treatment of bacterial infectious disease and inflammation caused by an infection.

The invention relates to the technical field of biomedicine, in particular to an umbilical cord mesenchymal stem cell exosome and a preparation method and application thereof.The umbilical cord mesenchymal stem cell exosome is derived from umbilical cord mesenchymal stem cells expressing S100g-MTOR binding domain fusion protein and can inhibit excessive autophagy through an S100g / MTOR signal channel, and therefore intestinal mucosal barrier damage caused by antibiotics is relieved. The preparation method comprises the following steps: constructing an expression vector, transfecting and screening a stable expression cell strain, performing serum-free culture, collecting a culture supernatant, purifying the exosome and the like. The exosome disclosed by the invention can be used for preparing a medicine for treating antibiotic-related intestinal mucosal barrier injury, has the advantages of strong targeting property, definite action mechanism, remarkable curative effect and the like, and provides a new choice for preventing and treating antibiotic-related intestinal adverse reactions.

The invention discloses a novel nano-drug as well as a preparation method and application thereof, and co-delivery of siRNA and 3BP is realized on an exosome nano-platform, so that a novel nano-drug Exo (at) siRNA / 3BP-CTX (ERBC) engineering exosome is generated. The surface-modified cetuximab (CTX) is targeted to tumors, and shows high targeting and low toxicity in imaging analysis. CCK-8, western blot and immunofluorescence detection are used for detecting the tumor killing effect and the synergistic effect. The ERBC finds that the occurrence of death-promoting autophagy in KRAS mutation tumors is promoted on the transmission electron microscope (TEM) and molecular level.

The invention discloses an application of a polypeptide in preparation of a medicine for preventing or treating cerebral apoplexy. The polypeptide comprises an amino acid sequence as shown in SEQ ID NO: 1. The N15 polypeptide expressed by the sequence can inhibit apoptosis and autophagy of damaged neurons by inhibiting expression of Tom20 and PARP proteins, promote expression of SYN-1 protein to promote a patient with ischemic cerebral apoplexy to recover learning, memory and cognitive functions, can comprehensively protect neuronal damage caused by ischemic cerebral apoplexy, can promote recovery of damaged nerve cells of an OGD model, and has a good application prospect. The polypeptide has an obvious protection effect on neuronal cells, the polypeptide is connected with a cell transmembrane carrier and can penetrate through a blood brain barrier to enter brain tissue to directly act on the neuronal cells, and an ischemic cerebral apoplexy animal model verifies that the polypeptide has an effect of treating cerebral apoplexy. Particularly, the cognitive ability and learning and memory ability of a mouse with cerebral arterial thrombosis can be recovered to a normal level, and a good treatment effect on cerebral arterial thrombosis is achieved.

The invention belongs to the technical field of biological medicines, and particularly relates to application of a PDCD4 expression inhibitor in preparation of a traumatic craniocerebral injury repair medicine. The research finds that the PDCD4 gene is positioned in a cell nucleus of an astrocyte, the PDCD4 is transferred to cytoplasm after traumatic craniocerebral injury, and an inhibition effect is generated on autophagy. Based on the research result, the invention provides the sh-PDCD4 lentivirus which is relatively good in lentivirus infection effect and can stably knock down the expression level of PDCD4, and the sh-PDCD4 lentivirus remarkably promotes the autophagy of astrocytes and has a relatively good application prospect on targeted drugs for clinically developing and treating traumatic craniocerebral injury.

The invention relates to autophagy extracellular vesicles capable of swallowing viruses as well as a preparation method and application of the autophagy extracellular vesicles, and relates to the technical field of biological medicines. The preparation method of the high-purity autophagy extracellular vesicles is provided for the first time, the purity of the high-purity autophagy extracellular vesicles exceeds 85%, and the high-purity autophagy extracellular vesicles are rich in various protein markers. CVB4 virus is used for infecting the autophagy extracellular vesicles, it is found that compared with common extracellular vesicles, the autophagy extracellular vesicles contain complete CVB4 virus particles, and the virus particles carried by the autophagy extracellular vesicles can more effectively infect brain and spleen tissue of a mouse, so that the autophagy extracellular vesicles can be used for treating the brain and spleen tissue of the mouse; therefore, the autophagy extracellular vesicle disclosed by the invention can break through the limitation of a host virus specific receptor, efficiently infect corresponding tissues and enlarge the virus tissue tropism. Therefore, the autophagy extracellular vesicle disclosed by the invention can be used for screening antiviral drugs, and also has important significance for constructing a virus infected animal model.

The invention relates to the technical field of nano medicine, in particular to iron gambogic acid adriamycin nano particles, a preparation method and application. Gambogic acid, doxorubicin hydrochloride and an iron-containing compound are matched, carbonyl oxygen and hydroxyl in the gambogic acid and Fe < 3 + > form coordinate bonds, carbonyl and amino in the doxorubicin hydrochloride and a conjugated system in a benzene ring are combined with Fe < 3 + > through covalent bonds and pi-pi accumulation, a core-shell structure is formed, and the iron gambogic acid doxorubicin nano-particles are obtained. The particle size of the iron gambogic acid adriamycin nano-particles ranges from 2 nm to 10 nm. The iron gambogic acid adriamycin nano-particles have excellent multi-enzyme activity, autophagy amplification is effectively achieved, traditional chemotherapy drug resistance caused by autophagy is overcome, and the glioma chemotherapy effect is improved.

The invention relates to a composition with whitening and skin tendering effects, essence lotion and a preparation method and application of the essence lotion, in the composition with the whitening and skin tendering effects, the weight ratio of achillea millefolium extract to hydroxyethyl piperazine ethanesulfonic acid to ormosia henryi bark extract is (0.1-1): (0.5-8): (0.05-0.5). The essence lotion is prepared from the following components in percentage by weight: 0.75 to 6 percent of composition with skin whitening and tendering effects, 3 to 8 percent of emollient, 0.5 to 2 percent of emulsifier, 0.01 to 1 percent of filler, 0.5 to 2 percent of thickener, 0.8 to 1.5 percent of preservative, 0.02 to 0.2 percent of daily essence, 0.1 to 10 percent of skin conditioner, 0.1 to 10 percent of humectant and the balance of deionized water. The main components have a synergistic effect, can effectively regulate and control the information network effect of endothelin and reduce the differentiation, proliferation and the like of melanocytes in the first step of melanin generation by inhibiting the activity of tyrosinase, can promote cell autophagy and effectively metabolize melanin and lipofuscin generated in the skin, and achieves the effects of whitening, whitening, whitening and the like. The skin tendering effect is realized.

The invention discloses an unmanned aerial vehicle remote sensing quantitative marine ecological restoration effect evaluation system, and relates to the technical field of marine ecological monitoring and evaluation, and the system comprises a rhythm resonance excitation module, a resonance coupling inversion module, an error autophagy verification module, and a resonance steady state adjustment module. According to the invention, a three-in-one framework of a rhythm resonance coupling mechanism, an error autophagy verification mechanism and a rhythm phase latch-cross-scale energy anchoring stator mechanism is utilized, and four core modules of rhythm resonance excitation, resonance coupling inversion, error autophagy verification and resonance steady state adjustment are combined. The method solves the problems of low evaluation precision, no self-verification capability and large errors caused by marine environment interference due to manual sampling recheck and rhythm splitting in the prior art, realizes quantitative evaluation and self-verification of the marine ecological restoration effect without manual sampling, remarkably improves evaluation efficiency, credibility and precision, and is suitable for large-scale popularization and application. And micro-scale power data can be synchronously output, the endurance of the unmanned aerial vehicle is prolonged, and the restoration trend is pre-judged.

The invention relates to a biological medicine technology, and discloses chloroquine lipid, a preparation method and application thereof, and lipid nanoparticles containing the chloroquine lipid. The chloroquine lipid is synthesized from chloroquine substances and alkylene oxide through a ring-opening reaction, and the chloroquine substances are selected from at least one of chloroquine, hydroxychloroquine, chloroquine derivatives and hydroxychloroquine derivatives. The lipid nanoparticles contain the chloroquine lipid, cationic lipid, phospholipid, PEG (polyethylene glycol) lipid and cholesterol. The chloroquine lipid can be used as a lipidated autophagy inhibitor, can endow the LNPs carrier with an autophagy inhibition function, and realizes the autophagy inhibition effect of'time-space synchronization ', so that the obstacle of'autophagy lysosome degradation' is broken through, and the gene delivery efficiency is enhanced.