2325 results about "Plant genetic engineering" patented technology

The present invention relates to the use of MtNAC33 gene and a protein encoded thereby in the cultivation of high-biomass and drought-tolerant alfalfa, belonging to the technical field of plant genetic engineering. The plant protein encoded by MtNAC33 gene has the amino acid sequence set forth in SEQ ID NO: 2, and MtNAC33 gene has the nucleotide sequence set forth in SEQ ID NO: 1. In the present invention, the drought tolerance of Arabidopsis thaliana and alfalfa can be significantly improved by regulating the expression level of MtNAC33 in alfalfa. The phenotypes of delayed flowering, increased leaf-to-stem ratio and more biomass in alfalfa were also developed, which has an important effect for genetic improvement of stress resistance and biomass of forage grass and other crops.

The invention belongs to the field of plant genetic engineering, and discloses application of a vitis amurensis cold-resistant gene VaWRKY65 in plant cold-resistant genetic improvement, the VaWRKY65 gene is a transcription factor separated and cloned from extremely cold-resistant vitis amurensis, and the sequence of the VaWRKY65 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the vitis amurensis cold-resistant gene VaWRKY65. The gene is used for constructing an overexpression and interference vector, the overexpression and interference vector is respectively introduced into arabidopsis thaliana and vitis amurensis through agrobacterium tumefaciens-mediated genetic transformation, and biological function verification of the obtained transgenic plant shows that the cloned VaWRKY65 gene has the function of improving the cold resistance of the plant. The discovery of the gene provides a new gene resource for plant stress resistance molecular design breeding and provides a new genetic resource for implementation of green agriculture and water-saving agriculture, and development and utilization of the genetic resource are beneficial to reduction of agricultural production cost and realization of environmental friendliness.

The invention provides a Chinese cabbage clubroot susceptibility related gene BrSWEET14a, a construction method and application, belongs to the technical field of plant genetic engineering, and particularly relates to a Chinese cabbage clubroot susceptibility related gene BrSWEET14a. The CDS sequence of the Chinese cabbage BrSWEET14a is as shown in SEQ ID No. 1 (sequence identifier number 1). The gene is transformed into Col-type arabidopsis thaliana through an agrobacterium flower immersion transformation method, a BrSWEET14a heterologous expression arabidopsis thaliana strain is obtained, and the result shows that T-DNA insertion mutant of the BrSWEET14a homologous gene Atsweet14 is more resistant to clubroot than that of the Col-type arabidopsis thaliana, and heterologous expression of Chinese cabbage BrSWEET14a leads to reduction of disease resistance of arabidopsis thaliana to plasmodiophora brassicae. Therefore, the Chinese cabbage BrSWEET14a is closely related to the clubroot disease, and the gene and the homologous gene of the gene in other cruciferous plants can be applied to breeding of the cruciferous plants and other horticultural plants and have a good application prospect.

The invention discloses application of an OsNLP3 gene, and relates to the technical field of plant genetic engineering, and an amino acid sequence coded by the OsNLP3 gene is shown as SEQ ID NO.2. By constructing an OsNLP3 gene overexpression plant and comparing the OsNLP3 gene overexpression plant with a wild type of the OsNLP3 gene overexpression plant, the increase of the single gene expression quantity of the OsNLP3 gene overexpression plant causes the significant increase of the total protein content of the rice, the invariable characters of the grain length, the grain width, the yield and the like, the genetic effect is significant, and the OsNLP3 gene overexpression plant has huge application potential and prospects for the improvement of the characters of rice varieties and provides a new gene resource for

The invention discloses a wheat E3 ubiquitin ligase gene RFEL1 and application of the wheat E3 ubiquitin ligase gene RFEL1 in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The invention discloses a nucleotide sequence of a wheat E3 ubiquitin ligase gene RFEL1 as shown in SEQ ID NO. 3. The invention further provides application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof. The application comprises any one of the following applications: A1, improving the resistance of wheat to stripe rust; a2, the resistance of wheat to powdery mildew is improved; and A3, the resistance of wheat to leaf rust is improved. The E3 ubiquitin ligase RFEL1 gene provided by the invention not only has broad-spectrum resistance to different stripe rust, but also has resistance to wheat leaf rust and powdery mildew. The E3 ubiquitin ligase RFEL1 gene disclosed by the invention is applied to broad-spectrum resistance breeding of wheat and has important application value.

The invention provides a Ustilaginoidea virens gene UvASE2 for regulating and controlling rice resistance and application of the Ustilaginoidea virens gene UvASE2, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the Ustilaginoidea virens gene UvASE2 is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the Ustilaginoidea virens gene UvASE2 is as shown in SEQ ID NO: 2. By constructing a transgenic rice plant for heterologous expression of UvASE2, it is found that the transgenic rice can significantly improve the resistance to false smut and bacterial blight and significantly induce the outbreak of active oxygen. The heterologous expression UvASE2 gene of Ustilaginoidea virens has the function of positively regulating and controlling the disease resistance of rice, and the UvASE2 gene can be used for improving the disease resistance of rice and has important significance on creation of disease-resistant germplasm of rice.

The invention belongs to the technical field of plant genetic engineering, and discloses an application of a corn gene ZmGG1 in control of corn yield, a protein encoded by the ZmGG1 is shown in SEQ ID NO.2, and the gene is located in the ninth chromosome of corn and used for controlling the row number and the ear weight of corn ears. The gene is knocked out by using a CRISPR / Cas9 technology, gene expression is inhibited, and the ear row number and the ear weight of corn can be increased.

The invention relates to the technical field of plant genetic engineering, in particular to an application of an alfalfa MsPL19 protein and a gene thereof in regulating and controlling the plant height, fresh weight or biological yield of alfalfa. The invention provides an MsPL19 protein, a gene and application thereof, which can obviously improve the plant height, the biological yield and the fresh weight of medicago sativa and further improve the quality of the medicago sativa. Meanwhile, it is found that the MsPL19 protein and gene can respond to low temperature, drought, salt, hormone and other pathways, the protein interacting with MsPL19 is obtained through immunoprecipitation-mass spectrometry analysis, corresponding molecular modules can be enriched, the molecular mechanism of alfalfa MsPL19 responding to the low-temperature environment is analyzed, new cold-resistant varieties can be bred for alpine regions, and the application prospect is broad. And a new breeding direction is provided for increasing the yield of alfalfa.

The invention discloses an application of a medicago sativa MsDGK5 gene and a protein coded by the medicago sativa MsDGK5 gene in salt stress resistance of plants, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the medicago sativa MsDGK5 gene provided by the invention is as shown in SEQ ID NO. 1. According to the application, MsDGK5 gene overexpressed arabidopsis thaliana and medicago sativa transformation plants are constructed, growth and development indexes and physiological and biochemical parameters of the plants under salt stress are systematically measured, and it is proved that overexpression of the MsDGK5 gene can significantly improve the salt tolerance of the transgenic plants. Based on the experimental results, the key regulation effect of the MsDGK5 gene in plant salt tolerance is defined for the first time, an important gene resource is provided for crop salt tolerance genetic improvement, and a new technical approach is also provided for coping with the increasingly aggravated soil salinization problem.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention discloses an application of IbWRKY26 protein and a coding gene thereof in regulation and control of sweet potato soft rot resistance, and belongs to the technical field of plant genetic engineering. The invention provides an application of the IbWRKY26 protein in any one of the following items: (1) an application in regulation and control of sweet potato soft rot; (2) application in preparation of products for regulating and controlling sweet potato soft rot; (3) application in soft rot resistant sweet potato breeding; (4) application in cultivation of soft rot resistant sweet potatoes; the amino acid sequence of the IbWRKY26 protein is as shown in SEQ ID NO. 2. Experiments prove that compared with a wild type sweet potato, the soft rot disease resistance of the overexpressed IbWRKY26 gene is remarkably improved. The IbWRKY26 protein and the coding gene thereof provided by the invention have important theoretical significance and application value in the research on the soft rot resistance of sweet potatoes.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a method for establishing an elymus nutans seed callus induction and regeneration system. The invention provides a tissue culture medium and a tissue culture method for culturing elymus nutans, and in the tissue culture medium provided by the invention, 2, 4-D can induce explants to form calluses and promote cell division and growth. The CH has a good effect on differentiation of embryoid and adventitious buds. ABA can promote the development of seed embryos. The KT can promote the generation of calluses and the growth of adventitious buds. NAA can promote plant growth and promote generation of adventitious roots. Under the combined action of NAA, 2, 4-D, ABA, KT and CH, the callus induction rate of the mature embryo is improved. The invention also provides a regeneration tissue culture method of elymus nutans, the mature embryo is used as an explant for tissue culture, and the callus induction rate of the mature embryo is high.

The invention discloses a rice OsFb34 gene as well as an encoding protein and application thereof, and relates to the technical field of plant genetic engineering. The CDS nucleotide sequence of the rice OsFb34 gene disclosed by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein of the rice OsFb34 gene is as shown in SEQ ID NO.2. The method comprises the following steps: constructing a knockout vector of a rice OsFb34 gene; transforming the constructed knockout vector into rice; and cultivating and screening to obtain a transgenic rice plant with reduced rice grain length, increased grain width and reduced length / width ratio. The mutants of osfb34-1 and osfb34-2 both show phenotypes that the grain length is reduced and the grain width is increased, so that the ratio of the length / width ratio is reduced. The result shows that the OsFb34 gene deletion can cause the reduction of the grain length and the increase of the grain width of the rice, and further cause the reduction of the length / width ratio.

The present disclosure is in the field of plant genetic engineering. Specifically, the invention relates to application of a mutant GmLn protein and an encoding gene thereof in regulation and control of soybean yield, in particular to but not limited to a method for obtaining soybeans capable of being densely planted, a method for detecting the soybeans capable of being densely planted, related primers and kits, and further relates to soybean plants capable of being densely planted and a method for improving soybean planting density. According to the method provided by the invention, the soybean target sequence is subjected to gene editing by expressing the gene editing tool in the soybean, so that the soybean which is improved in yield and can be densely planted is obtained.

The invention relates to a mulberry MaBEH1 / 2 gene for improving plant salt tolerance and application thereof, and belongs to the technical field of plant genetic engineering and biology. Wherein the coding nucleotide sequence of the mulberry MaBEH1 / 2 gene for regulating and controlling the salt tolerance of the woody plant is shown as a sequence 3; and the amino acid sequence of the MaBEH1 / 2 expression protein is shown as a sequence 4. According to the invention, MaBEH1 / 2 is transferred into 84K poplars to obtain MaBEH1 / 2-OE transgenic poplars; compared with a non-transgenic poplar, the salt stress tolerance of the MaBEH1 / 2-OE transgenic poplar can be remarkably enhanced. The invention provides key gene resources and technical support for genetic improvement of salt-tolerant forest varieties.

The invention discloses application of a rice gene SLL4 in regulation and control of a rice leaf shape, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the SLL4 gene is as shown in SEQ ID NO. 1; the rice gene SLL4 regulates and controls the development of blister cells and thick-walled cells so as to influence the growth form of leaves, and overexpresses the SLL4 gene in rice, so that the curling degree of the leaves can be increased. The method disclosed by the invention is applied to production practice, is beneficial to shaping ideal plant types of rice and increasing the yield of crops, and has an important application value.

The invention discloses an application of an ABC transporter family gene OsABCA3 in regulation and control of plant breeding. Belongs to the field of plant genetic engineering. According to the application of the ABC transport protein family gene OsABCA3 in plant breeding regulation and control, the amino acid sequence of the protein encoded by the ABC transport protein family gene OsABCA3 is shown as SEQ ID No: 2. According to the invention, the ABC transporter gene OsABCA3 is knocked out in rice, so that the Cd absorption in a knockout plant under Cd stress is obviously reduced compared with that of a wild type plant, the Cd content of grains in the knockout plant planted in Cd-polluted soil is obviously reduced compared with that of the wild type plant, but the content of essential elements in the grains and the yield of rice are not negatively influenced; therefore, a new candidate gene resource is provided for creating safe rice with low Cd grains, and a potential repair method is also provided for rice Cd pollution treatment.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a wheat magnesium transporter TaNIPA8-6B and application of a coding gene thereof to regulation and control of plant stripe rust resistance. Functional analysis is carried out on the wheat magnesium transporter TaNIPA8-6B by means of gene editing and overexpression, and it is found that the wheat magnesium transporter TaNIPA8-6B induces expression in both affinity and non-affinity system reactions of wheat and puccinia striiformis, so that a mutant plant of the gene editing and overexpression TaNIPA8-6B gene is created; according to the TaNIPA8-6B gene, the gene knockout mutant of the TaNIPA8-6B gene is identified to show susceptibility to stripe rust non-compatible races CYR23, the over-expression mutant plant shows disease resistance to stripe rust compatible races CYR32, the fact that the TaNIPA8-6B gene plays a positive regulation role in the disease resistance of wheat to stripe rust is clear, and a high-quality germplasm resource is provided for stripe rust resistance of wheat.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a KASP marker for detecting yellow 6106 in transgenic soybeans and application of the KASP marker. The specific sequence where the KASP marker is located comprises ZH6106-2R and / or ZH6106-3R, and the specific sequence where the KASP marker is located comprises ZH6106-2R; the nucleotide sequence of the ZH6106-2R is as shown in SEQ ID NO: 1; the nucleotide sequence of the ZH6106-3R is as shown in SEQ ID NO: 2. Through the KASP marker and the primer, whether a sample to be detected contains the yellow 6106 component in the transgenic soybean or not can be effectively detected, and the detection method is rapid, simple, convenient, accurate and reliable.

The invention relates to the technical field of plant genetic engineering, and particularly discloses an alfalfa salt-alkali-resistant and low-temperature composite stress-resistant gene MsNCED5 and application of encoded protein thereof in improving salt-alkali-resistant and low-temperature composite stress-resistant capability of plants. The nucleotide sequence of the gene disclosed by the invention is as shown in SEQ ID NO.1, the full length is 1833 bp, and 610 amino acids are encoded. According to the invention, an MsNCED5 overexpression vector is constructed and medicago sativa is converted, so that an MsNCED5-OE transgenic line is successfully created, and the MsNCED5 gene expression quantity of the MsNCED5-OE transgenic line is obviously increased compared with that of a wild type. Under the salt-alkali and low-temperature composite stress, the transgenic plant has stronger stress tolerance compared with the wild plant, which indicates that the overexpression of the MsNCED5 gene improves the resistance of the plant to the salt-alkali and low-temperature composite stress. The MsNCED5 gene disclosed by the invention provides an important theoretical basis for researching an alfalfa stress resistance molecular mechanism and breeding.

The invention discloses a rice salt stress resistant gene OsHAK11 coding protein and application thereof, and belongs to the field of plant genetic engineering. According to the method, a rice OsHAK11 gene (the nucleotide sequence is shown as SEQ ID NO.1) is knocked out through a CRISPR / Cas9 gene editing technology, and a mutant with significantly enhanced salt tolerance is obtained. The gene editing vector pEGCas9Pubi-B-OsHAK11 contains sgRNA of a region as shown in a target SEQ ID NO.4, a rice receptor material is transformed through agrobacterium tumefaciens mediation, and a plant with the OsHAK11 gene subjected to frame shift mutation is obtained through screening. A salt stress experiment shows that the survival rate of the mutant oshak11 is obviously higher than that of a wild type. The invention provides a new gene resource and an efficient technical means for salt-tolerant breeding of rice.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a centella asiatica glycosyl transferase gene in catalyzing glycosylation of asiatic acid and madecassic acid. The nucleotide sequence of the gene is shown as SEQ ID No.1, and the protein coded by the gene can catalyze the C-28 site of asiatic acid and madecassic acid to perform glucose group modification so as to generate asiatic acid monoglucoside and madecassic acid monoglucoside, and preferentially catalyzes madecassic acid. By constructing a metabolic regulation network, the genes are widely and accurately screened, and the glycosylation process of catalyzing asiatic acid and madecassic acid is analyzed; an important reference basis is provided for improving the glycosylation efficiency of asiatic acid and madecassic acid and the yield of asiaticoside and madecassoside in the in-vitro synthesis and industrial production process of the centella asiatica triterpenoid saponin, and an important theoretical support is provided for large-scale production of the centella asiatica active saponin.

The invention belongs to the field of plant genetic engineering, and particularly relates to an OsPIGL gene and a regulation and control method thereof for improving the grain length, thousand grain weight and drought tolerance of rice. The nucleotide sequence of the OsPIGL gene is as shown in SEQ ID NO: 1, and the CDS sequence is as shown in SEQ ID NO: 2; the specific application is that overexpression of the OsPIGL gene effectively improves the grain length, the thousand seed weight and the drought tolerance of the rice, and new gene resources and technical means are provided for rice breeding. The gene and the application of the gene provided by the invention are helpful for cultivating a new rice variety with excellent agronomic traits and stress resistance and improving the yield and quality of rice, and have important significance for guaranteeing grain safety.

The invention relates to the technical field of plant genetic engineering, in particular to a promoter and application thereof in orchid plants. The invention provides an orchid endogenous promoter which is a powerful constitutive promoter and can drive a target gene to be powerfully expressed in various tissues such as roots, stems, leaves and flowers of an orchid. Compared with a CaMV 35S promoter, the promoter has the advantages that the driving efficiency of the gene in orchid plants is greatly improved, an important tool is provided for molecular breeding of plants, and the promoter has a relatively good application prospect.

The invention discloses a pinus massoniana PmYTH1 gene as well as an encoding protein and application thereof, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the pinus massoniana PmYTH1 gene disclosed by the invention is as shown in SEQ ID NO. 1. The method comprises the following steps: constructing an expression vector of a pinus massoniana PmYTH1 gene, and transforming the expression vector into an arabidopsis thaliana mutant; and cultivating and screening to obtain a transgenic arabidopsis thaliana plant of which the ABA tolerance is obviously increased. The result shows that the tolerance of the arabidopsis thaliana ect2 / 3 / 4 mutant to ABA is improved by overexpressing the PmYTH1 in the arabidopsis thaliana ect2 / 3 / 4 mutant; the expression quantity of ABA signal channel genes is obviously increased. The mRNA of the ABA signal channel gene in the 35S:: PmYTH1 / ect2 / 3 / 4 strain is more stable. The invention provides a molecular means and basis for cultivating a new pinus massoniana variety with high ABA and adversity stress tolerance.

The invention belongs to the technical field of plant genetic engineering molecular breeding, and particularly relates to application of wheat TabHLH93 protein or a coding gene thereof in regulation and control of plant grain development. According to the invention, a binary expression vector capable of realizing ectopic expression of the TabHLH93 gene is constructed and is genetically transformed into common wheat to obtain a progeny with high expression of the TabHLH93 gene, so that overexpression of the TabHLH93 gene is realized, and it is verified that the TabHLH93 has a function of regulating and controlling the size of wheat grains and also has an important regulation and control effect on agronomic characters such as wheat plant height, tiller number, spike length and thousand seed weight. A candidate gene with an important application prospect is provided for wheat high-yield genetic improvement, and the gene has important significance for guaranteeing grain safety.

The invention discloses a SbWRKY51 gene, a promoter and application of the SbWRKY51 gene and the promoter in improvement of salt tolerance of sorghum, and belongs to the technical field of plant genetic engineering. The SbWRKY51 gene is screened from sorghum, and the expression level of the SbWRKY51 gene is up-regulated after salt stress treatment. The protein coded by the gene belongs to a WRKY transcription factor family, has transcriptional activation activity, and can start the expression of a reporter gene. Sorghum overexpression strains and complementary strains of the gene are obtained, salt tolerance related physiological indexes are measured, overexpression of the gene can improve the seed germination rate, increase the seed root length, regulate ion balance and enhance the free radical scavenging capacity of plants, and the effect of the gene in the aspect of improving the salt tolerance is embodied. A further research finds that the SbWRKY51 gene can enhance the plant salt tolerance by regulating a lignin synthesis pathway. The technical scheme provides a basis for cultivating resistant plants, and has important guiding significance for enhancing the production potential of sorghum under a high-salt condition and promoting agricultural development.

The invention belongs to the technical field of plant gene engineering, and particularly relates to an OsNUC1 gene and application of a gene mutant constructed by the OsNUC1 gene to regulation and control of rice plant height, leaf length and panicle type. The regulation is to regulate the plant height and leaf growth of the rice and influence the shape of the ear part. Compared with the prior art, the OsNUC1 gene mutant has the following advantages: (1) the OsNUC1 is directionally edited by utilizing a CRISPR / Cas9 gene editing technology, an OsNUC1 gene mutant rice material is obtained, the biological phenotype of the OsNUC1 gene mutant rice material is investigated, the phenotype investigation on T1-generation seedlings of the OsNUC1 shows that the plant heights and the leaf lengths of one-week-old and two-week-old seedlings of the mutant are obviously higher than those of wild seedlings, the leaf widths have no obvious difference, and the OsNUC1 gene mutant rice material has a good application prospect; the result shows that the OsNUC1 can regulate the plant height and the leaf growth of the rice and influence the shape of the ear part. (2) it is proved that the nucleolin gene OsNUC1 is negatively regulated by a transcription factor OsARF7, and the phenotypes such as the plant height, the leaf length, the grain length and the grain width of the OsNUC1 / OsARF7 mutant are more obvious than those of the OsNUC1 mutant. The invention further proves the application of the OsNUC1 gene in regulation and control of rice plant height and leaf growth.

The invention discloses a poplar PdbHLH57 gene as well as an expression protein and application thereof, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the poplar PdbHLH57 gene disclosed by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the expression protein of the poplar PdbHLH57 gene is as shown in SEQ ID NO.2. The method comprises the following steps: constructing an expression vector of a poplar PdbHLH57 gene, and transforming the expression vector into a poplar leaf; and cultivating and screening to obtain the transgenic poplar with red leaves. Results show that compared with wild poplar, the PdbHLH57 gene for promoting poplar anthocyanin synthesis has the advantages that transgenic poplar leaves become red, and the anthocyanin content in transgenic plant leaves is remarkably increased; the expression level of anthocyanin biosynthesis related genes is remarkably increased, and further, it is shown that the PdbHLH57 gene plays an important role in poplar anthocyanin biosynthesis.

The invention relates to the technical field of plant genetic engineering, in particular to a gene of leymus secalinus participating in adversity stress and application of the gene. The gene LsHSFA participating in adversity stress in leymus secalinus is found for the first time, the nucleotide sequence of the gene LsHSFA is shown as SEQ ID NO.1, and the amino acid sequence of the coded protein is shown as SEQ ID NO.2. Through cloning and identification of the adversity stress participating gene of the leymus secalinus, expression analysis of the gene, genetic transformation of the gene and other means, the function of the gene is analyzed and verified, and the result shows that the adversity stress participating gene of the leymus secalinus has important influence on promotion of upper development of the leymus secalinus and improvement of heat resistance, salt resistance, drought resistance and other stress resistance; the stress-resistant gene of the leymus secalinus is utilized to positively regulate and control the abiotic stress function, so that the leymus secalinus can cope with various stress adversities better, the leymus secalinus can adapt to more complex and harsh ecological environments, degenerated and desertified grassland is improved, and potential application prospects are provided for improved variety breeding and excellent population construction of the leymus secalinus in the future.