57results about How to "Improve growth performance" patented technology

The invention discloses preparation and application of a porcine interleukin 17, 22 and 29 co-expression biological agent. The invention provides a fusion protein. The fusion protein comprises a porcine interleukin 17, a porcine interleukin 22 and a porcine interleukin 29. The recombinant yeast Po1h-IL17 / 22 / 29 capable of expressing porcine IL-17, IL-22 and IL-29 fusion molecules is constructed, and cells and mice are treated by the recombinant yeast Po1h-IL17 / 22 / 29, so that the cells and the mice obtain higher immune resistance to virus and bacterial infection. Subsequently, the piglets are orally inoculated with Po1h-IL17 / 22 / 29, and it is proved that the Po1h-IL17 / 22 / 29 obviously improves the growth performance and the whole body immunity of the piglets.

This invention relates to the field of plant genetic engineering technology, and more particularly to... SsNHX1 and SsSOS2 Dual-gene plant expression vector and its application in improving salt tolerance in alfalfa. The plant expression vector uses pCAMBIA1300 as its backbone and inserts genes derived from Suaeda salsa. SsNHX1 Genes and SsSOS2 It is constructed from genes. SsNHX1 Genes and SsSOS2 The CDS coding sequence of the gene is shown in SEQ ID NO:1 and SEQ ID NO:2 of the sequence listing. Using Agrobacterium tumefaciens EHA105-mediated transformation, the vector was introduced into the leaves of sterile alfalfa seedlings. Positive transgenic plants were obtained through co-culture, callus induction differentiation, and PCR identification. The advantage lies in the synergistic effect of the two genes, which effectively enhances the salt tolerance of alfalfa, enabling it to grow normally under 200–300 mM NaCl stress, providing a new solution for forage cultivation in saline soils.

The application belongs to the technical field of biology and particularly relates to a molecular marker for predicting daily weight gain of pigs, a primer pair, a kit and application thereof. The nucleotide sequence of the molecular marker is shown as SEQ ID NO. 3, and the 474th site at the 5' end of the molecular marker is a polymorphic site, the base of which is C or G, and the daily weight gain of the pig with the genotype CC of the site is higher than that of the pig with the genotype GG. The molecular marker provided in the application is a molecular marker related to the growth performance of pigs, and is used for predicting the daily weight gain, feed conversion rate and other growth traits of pigs, and provides an accurate genetic basis for early selection of pigs.

This disclosure relates to a compound composition comprising stearic acid and moclobemide, which can effectively improve the drought resistance of crops and promote crop growth and development under drought stress. This disclosure also provides uses of the composition and methods for improving crop growth and development under drought stress.

The invention relates to the technical field of plant genetic engineering and agricultural planting, in particular to application and method of an SlGRXC9 gene in improvement of tomato cadmium stress tolerance, the number of the SlGRXC9 gene is Solyc07g053550, the core coding region sequence of the SlGRXC9 gene is SEQ ID NO.1, and the coded protein sequence is SEQ ID NO.2; through construction of SlGRXC9 overexpression transgenic tomatoes, heterologous expression of protein encoded by the gene in plant cells or use of the gene as a molecular breeding target, the system activity of antioxidant enzymes such as SOD, POD, CAT and GR in tomatoes can be enhanced, cadmium stress induced H2O2 and O2-accumulation and membrane lipid peroxidation damage are reduced, and absorption and accumulation of the tomatoes to cadmium ions are not affected; according to the method, the growth ability and physiological stability of the tomatoes under cadmium stress are remarkably improved, the risk of nutrient imbalance is avoided, the hereditary stability is high, and the method can be widely applied to safe production of the tomatoes in cadmium-contaminated soil and creation of stress-tolerant germplasm.

This invention discloses the application of a porcine-derived Bacillus in antioxidant activity, wherein the porcine-derived Bacillus is used to prepare antioxidant products. The porcine-derived Bacillus is Bacillus subtilis YB-X-721, classified as Bacillus subtilis, and was deposited at the China General Microbiological Culture Collection Center on November 10, 2023, with accession number CGMCC No. 28942. The fermentation supernatant, bacterial suspension, and cell-free extract of the porcine-derived Bacillus subtilis YB-X-721 of this invention all possess certain DPPH free radical, superoxide anion free radical, and hydroxyl free radical scavenging abilities, as well as a certain reducing ability, and can be used to prepare antioxidant products. Furthermore, when the porcine-derived Bacillus subtilis YB-X-721 of this invention is added to piglet feed as an animal feed additive, it can improve the growth performance of weaned piglets, significantly increasing average daily weight gain, and significantly reducing average daily feed intake, feed conversion ratio, and diarrhea rate.

The application discloses a kind of fermented feed fermented by three strains of probiotics of Bacillus YA ( Bacillus sp ), yeast Y ( Saccharomyces cerevisiae ) and capsular red bacteria R3 ( Rhodobacter capsulatus ), and preparation method and application in aquaculture thereof, and viable count is not less than 1×10 7 CFU / g.The fermented feed is replaced ordinary feed according to certain proportion, and feeding result shows that aquaculture water quality is significantly improved after adding fermented feed, and fish growth performance, digestive enzyme activity, immunity, and muscle quality are also obviously improved.Using the feed in suitable range can shorten growth cycle, reduce cost, improve aquaculture environment, reduce antibiotic use, and improve muscle quality of farmed animals, with good economic and ecological benefits, simple fermentation process, low cost, and suitable for industrial large-scale production.

The application discloses a process for improving the quality of palm kernel meal feed by enzyme and bacteria synergistic fermentation, and belongs to the field of feed biotechnology.The process is characterized by the following steps: crushing and screening palm kernel meal, mixing the palm kernel meal with 20% bran and 4% ammonium sulfate as dry matter to obtain a basic substrate;compounding bacillus subtilis, aspergillus niger and saccharomyces cerevisiae at a ratio of 1:1:1 of viable bacterial number, adding 0.67% mannanase and 0.3% xylanase to prepare a bacteria-enzyme mixed solution, inoculating the bacteria-enzyme mixed solution at a 10% inoculation amount, adjusting the moisture content to 50% and the pH to 6.4, and then sealing and storing the bacteria-enzyme mixed solution for use after 4 days of fermentation at 33.5 DEG C; and the bacteria-enzyme mixed solution can be stored for a long time.The process can efficiently degrade crude fiber and anti-nutritional factors, improve the protein content and the digestibility, is stable and easy to mass-produce, can replace part of corn and soybean meal in the daily ration of ruminants, and realizes high-value utilization of palm kernel meal.

The application discloses application of Lactobacillus plantarum fermented whole-plant corn in improving growth performance, slaughtering performance and meat quality of black sheep, and belongs to the field of livestock feeding. The technical problem solved by the application is how to improve the growth performance, slaughtering performance and meat quality of livestock. It is found that after livestock is fed with Lactobacillus plantarum fermented corn prepared by using Lactobacillus plantarum 6H2, the average daily weight gain and daily feed intake are significantly improved, the feed conversion ratio is significantly reduced, the carcass weight is significantly improved, the meat shear force is significantly reduced, the number of white blood cells, the number of lymphocytes and the percentage of lymphocytes are significantly improved, the percentage of neutrophils is significantly reduced, and the content of total protein and albumin in serum is significantly improved. In summary, Lactobacillus plantarum 6H2 and the fermented corn obtained by using the same can improve the growth performance, slaughtering performance and muscle tenderness of livestock, improve the blood physiological and biochemical indexes, and improve the breeding benefit.

The application discloses a kind of fulvic acid hydrogen peptide biological stimulant hormone fertilizer and preparation method thereof, belong to fertilizer technical field.Fertilizer is by 15~30 parts of fulvic acid, 5~12 parts of peony seed meal small molecule peptide, 20~35 parts of potassium dihydrogen phosphate and other components compound, water is made up to 100 parts, and auxiliary agent is emulsifier and chelating agent compound.Preparation first by dissolving fulvic acid, potassium phosphate in water and obtaining mixed solution, then adding small molecule peptide and auxiliary agent and stirring until the system is stable;Small molecule peptide is prepared by peony seed meal defatted powder crushing and enzymolysis, solves the problem of traditional fertilizer nutrient antagonism, unstable system, and the utilization of peony seed meal also improves resource utilization.Tomato, cucumber seedling test shows that it can significantly improve the growth index of crop root system and aboveground part, and the root-promoting and seedling-strengthening effect is better than that of similar fertilizer on the market, and the preparation process is simple, easy to industrialization, and has good agricultural application prospect.

This invention provides a drug for antagonizing bacterial endotoxins, its preparation method, and its application, belonging to the biomedical field. The composition consists of 25-55 parts of dried powdered traditional Chinese medicine extract, 25-70 parts of mixed essential oil powder, and 40-90 parts of lentinan powder. The traditional Chinese medicine extract is prepared by decoction of Scutellaria baicalensis, Lonicera japonica, Forsythia suspensa, and Isatis indigotica under reduced pressure at low temperature and spray drying. The mixed essential oil powder is prepared by encapsulating peppermint oil and eucalyptus oil with gelatin and hydroxypropyl cellulose and then spray drying under high pressure. This invention, through specific formulation and processing, significantly enhances the synergistic antagonistic effect of each component against endotoxins. Experiments show that this composition exhibits antagonistic activity far superior to ibuprofen in vitro; in vivo, it significantly reduces the endotoxin content in the plasma of 817 broiler chickens, rapidly alleviates LPS-induced fever and diarrhea, and improves decreased growth performance. Therefore, this invention is of great significance for reducing antibiotic dependence and improving the efficiency of livestock and poultry farming.

The invention belongs to the technical field of fish culture, and particularly relates to bacillus coagulans, a traditional Chinese medicine fermentation preparation and application of the bacillus coagulans and the traditional Chinese medicine fermentation preparation. The technical problem to be solved by the invention is to provide a new way for improving excessive heavy metals in fish culture. The technical scheme of the invention is as follows: the bacillus coagulans DZNBC-06-16 is provided, and the preservation number of the bacillus coagulans DZNBC-06-16 is CGMCC (China General Microbiological Culture Collection Center) NO.35929. The strain can be subjected to anaerobic fermentation by utilizing a culture medium containing traditional Chinese medicines (honeysuckle and scutellaria baicalensis). The fermentation product is rich in flavones, butyric acid and lactic acid, and also can resist oxidation. The fermentation liquor is added into the fish basal feed, so that the influence of excessive heavy metals (copper, zinc and chromium) in the feed on the growth and shape indexes of zebra fish is remarkably improved.

The application provides a method for improving the LPS stress resistance of broilers, which is realized by feeding a mannose oligosaccharide compound preparation prepared from enzymolysis coffee grounds. The preparation method of the mannose oligosaccharide compound preparation comprises the following steps: crushing coffee grounds, and adding a compound reagent for pretreatment; adding a compound enzyme preparation I to the mixed system after reaction, and carrying out enzymolysis for 6-8 hours under the condition of 50-60 DEG C; then adding a compound enzyme preparation II, and carrying out sufficient enzymolysis under the condition of 55-65 DEG C; after the enzymolysis is completed, a fermentation compound microbial agent is used for continuing fermentation for 6-12 hours under the condition of 30-35 DEG C, and drying is carried out; the compound enzyme preparation I comprises papain, alpha-amylase and saccharifying enzyme; the compound enzyme preparation II comprises cellulase, mannanase and glucanase; and the fermentation compound microbial agent comprises maxwell kluyver yeast and lactobacillus plantarum. The method can not only avoid drug residues caused by feeding drugs, but also realize the reuse of coffee grounds.

This invention relates to recombinant mussel adhesive proteins, the nucleic acid molecules encoding them, nucleic acid constructs, expression vectors, recombinant engineered strains, pharmaceutical compositions, preparation methods, purification methods, and applications. This invention assembles and splices partial functional sequences from different mussels and type III collagen to obtain several novel mussel adhesive protein sequences. Further screening yielded recombinant mussel adhesive proteins Mfp5, Mfp55, Mfp131, and Mfp151, suitable for large-scale industrial production and exhibiting high expression levels. Efficacy experiments demonstrated that these proteins significantly promote cell migration, indicating their excellent cell repair function and broad application prospects.

This invention provides the application of polyferric sulfate in improving plant salt stress resistance and / or improving saline-alkali land, belonging to the field of agricultural technology. The polyferric sulfate application provided by this invention can effectively improve the germination ability of plant seeds under salt stress, effectively enhance the activity of plant antioxidant systems and photosynthetic efficiency, thereby effectively improving the survival rate and growth performance of plants under salt stress. Simultaneously, polyferric sulfate can effectively reduce the EC value of saline-alkali soil, thereby effectively improving the germination rate of plant seeds in saline-alkali land and effectively improving saline-alkali land. This invention is simple to operate, low in cost, and environmentally friendly, suitable for enhancing the salt and alkali tolerance of plants, and has broad application prospects.

The application discloses a macrobrachium rosenbergii three-dimensional flat plate recirculating aquaculture system and method, which comprises a system support, a three-dimensional flat plate aquaculture unit and a recirculating water treatment unit. The three-dimensional flat plate aquaculture unit comprises a plurality of flat plate water tanks arranged on the system support. A water inlet pipeline is arranged above the top of the flat plate water tank and supplies water to the flat plate water tank in a downward spraying manner. A water level control overflow port is arranged on the side wall of the flat plate water tank. A sewage discharge hole array is arranged on the bottom of the flat plate water tank. A water collecting tank is fixedly connected to the outside of the bottom of the flat plate water tank. The sewage discharge hole array is in communication with the water collecting tank. The water collecting tank and the overflow port are connected to a recirculating water pipeline. The recirculating water pipeline is connected to the input end of the recirculating water treatment unit. The output end of the recirculating water treatment unit is connected to the water inlet pipeline, thereby forming a closed loop circulation, and the specific growth rate and the weight gain rate of the macrobrachium rosenbergii are greatly improved.

This invention provides a *Roseolariomoraxella salina* strain for saline-alkali soil improvement, with accession number CGMCC No. 36067. The *Roseolariomoraxella salina* strain provided by this invention exhibits significant comprehensive application advantages. This strain demonstrates excellent survival and growth capabilities under saline-alkali stress, effectively reducing salinity and alkalinity in saline-alkali soils and thus improving the saline-alkali environment. Whole-genome analysis shows that this strain carries multiple functional genes related to osmotic regulation and ion transport, providing a molecular basis for its salinity-reducing ability. Adding citric acid or betaine can further enhance its salinity-reducing performance, and its biomass is significantly increased after optimization of fermentation conditions. This strain shows promising application prospects in saline-alkali soil improvement and saline-alkali aquaculture environment management.

The application discloses a kind of muscle development regulation zebra fish model construction method, it includes the following steps: by feeding N-acetylglucosamine to zebra fish, constructs O-GlcNAc glycosylation activation zebra fish model;And, by microinjection to zebra fish fertilized egg containing O-GlcNAc glycosidase gene fragment vector, constructs muscle-specific overexpression O-GlcNAc glycosidase zebra fish model.The application is verified bidirectionally to O-GlcNAc glycosylation function by constructing O-GlcNAc glycosylation activation model and muscle-specific OGA overexpression model, and it is revealed that O-GlcNAc glycosylation promotes muscle fiber thickening and whole fish protein deposition by up-regulating protein synthesis gene and muscle development regulatory factor.

The application discloses a kind of all mixed sheep feed and preparation method thereof, belong to the technical field of feed.The preparation method includes: using constant temperature and humidity closed treatment cooperates with compound detoxification agent to cottonseed for detoxification treatment, preparation rumen steady state amino acid composite carrier and cellulose carrier load composite enzyme, then modified cottonseed, composite carrier, load composite enzyme are mixed with basic mixture, after conditioning, granulation obtains all mixed sheep feed.The application is through mild detoxification process, and cottonin removal and protein protection are considered, and rumen nutrient release synchronization is realized using bentonite slow-release carrier and microcrystalline cellulose immobilized composite enzyme, and essential oil and enzyme preparation are protected from processing heat inactivation.The obtained feed realizes the efficient full replacement of cottonseed to soybean meal, significantly improves the growth performance and feed conversion efficiency of Bayinbuluke black head sheep, and reduces the breeding cost.

The invention discloses a multi-measure coupling improvement method for severe saline-alkali soil, which comprises the following steps: step 1, in the operation process of an irrigation system, performing continuous magnetoelectric treatment on brackish water for irrigation to obtain magnetoelectric treated brackish water; step 2, applying an organic fertilizer into a soil layer of the saline-alkali soil as a substrate improvement measure of a crop root growth area to obtain organic fertilizer saline-alkali soil; 3, plant growth promoting rhizosphere microorganisms are applied into the organic fertilizer saline-alkali soil, the plant growth promoting rhizosphere microorganisms and the organic fertilizer saline-alkali soil are fully mixed, and planting soil is obtained; 4, crops are planted in the planting soil, brackish water is treated through magnetoelectricity, and irrigation and field management are conducted according to local agricultural production conditions. According to the multi-measure coupling improvement method for the severe saline-alkali soil, the problems of high investment cost, long implementation period, high maintenance difficulty or unstable improvement effect in a saline-alkali soil improvement method are solved.

The application provides a sugarcane tissue culture method, and belongs to the technical field of sugarcane tissue culture, and the tissue culture method comprises the following steps: (1) taking sugarcane tender stems as explants, alcohol disinfection, and obtaining pretreated explants; (2) placing the pretreated explants in an induction culture medium, inducing culture, and obtaining callus; (3) placing the callus in a proliferation culture medium, proliferating culture, and obtaining adventitious buds; (4) placing the adventitious buds in a rooting culture medium, rooting culture, and obtaining tissue culture seedlings; (5) placing the tissue culture seedlings in a seedling raising substrate, seedling raising, and obtaining seedlings; the seedling raising substrate in step (5) comprises the following raw material components in mass parts: 10-20 parts of sugarcane residues, 40-60 parts of peat soil, 10-15 parts of sheep manure, and 8-10 parts of rice husk charcoal. The tissue culture method provided by the application can improve the rooting rate and transplanting survival rate of sugarcane tissue culture seedlings, and the growth traits of the tissue culture seedlings can also be significantly improved.

The system comprises a moving seat and a water storage tank arranged at the top end of the moving seat, supporting plates are fixed to the two sides of the top end of the moving seat, the two supporting plates are rotationally connected with the water storage tank through rotating shafts, swing assemblies for driving the water storage tank to move are arranged on one sides of the supporting plates, and a through groove is formed in the top end of the water storage tank; one side of the water storage tank communicates with a water pipe, and the end, away from the water storage tank, of the water pipe communicates with a spray head; according to the scheme, after underground water is conveyed into the water storage tank, the water storage tank is moved to a position with sufficient sunlight, the sunlight can heat water in the water storage tank through the lens, meanwhile, under the cooperation of the swing assembly, the water storage tank is driven to swing, the water storage tank drives water in the water storage tank to flow, and the water in the water storage tank is conveniently and rapidly heated; and then the flowers are irrigated through the spray heads, so that irrigation and growth of the flowers are facilitated, and the growth quality of the flowers is improved.

The invention discloses a single-bacterium fermentation compounding method of cattle feed, and relates to the technical field of feed fermentation. Comprising the following steps: preparing a single-bacterium fermentation bacterial material, the single-bacterium fermentation bacterial material comprises 42.25-47.25 parts of wheat bran, 19-21 parts of soybean meal, 14.25-15.75 parts of corn flour, 19-21 parts of rice hull powder and 0.003-0.005 part of a bacterial liquid, and strains used by the bacterial liquid comprise six probiotics including saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, lactobacillus plantarum, enterococcus faecalis and lactobacillus acidophilus; the six probiotics are subjected to independent solid fermentation, the prepared fermentation fungus materials are compounded in proportion to obtain a compound fermentation fungus material, and the compound fermentation fungus material is fed according to cows with different weights. Through the technical route of single-bacterium independent fermentation, the problem of mutual inhibition generated during compound fermentation of various strains is avoided, high-density propagation of the strains in the fermentation process is guaranteed, the compounded feed can meet the nutritional requirements of cattle in different stages, and the yield of the cattle is improved. The feed digestion and absorption rate is effectively improved, the cattle immunity is enhanced, and the growth performance is promoted.

The invention discloses application of a herba houttuyniae polysaccharide extract in a feed capable of improving intestinal health of piglets, the feed comprises the herba houttuyniae polysaccharide extract and a basic feed, and the application method of the herba houttuyniae polysaccharide extract comprises the following steps: adding the herba houttuyniae polysaccharide extract into the basic feed, and continuously feeding 21-28-day-old weaned piglets for 28 days, the intestinal flora structure of weaned piglets is adjusted through the houttuynia cordata polysaccharide extract, short-chain fatty acid secretion is promoted, and the intestinal morphology is improved, so that diarrhea prevention and intestinal health improvement are achieved; the herba houttuyniae polysaccharide extract is prepared from the following components in percentage by mass: 20 percent to 21.6 percent of herba houttuyniae polysaccharide, 5 percent to 10 percent of volatile oil, 25 percent to 35 percent of flavonoid, 10 percent to 15 percent of total amino acid, 3.1 percent to 6.1 percent of alkaloid, 3 percent to 10 percent of organic acid and 1.5 percent to 5.5 percent of sterol, and the herba houttuyniae polysaccharide is prepared from glucose, galactose, mannose, arabinose and xylose. The invention aims to improve the survival rate of early weaned piglets, shorten the breeding cycle of a farm and improve the reproductive performance.

The invention discloses a compound probiotic preparation for relieving heat stress of sows and application of the compound probiotic preparation, and belongs to the technical field of animal feed. The compound probiotic preparation comprises clostridium butyricum, bacillus subtilis, bacillus licheniformis and enterococcus faecalis, wherein the final concentration of the clostridium butyricum in the feed is 1 * 10 < 6 > CFU / g, and the final concentration of the bacillus subtilis, the final concentration of the bacillus licheniformis and the final concentration of the enterococcus faecalis in the feed is 5 * 10 < 6 > CFU / g. The preparation provided by the invention can effectively reduce the stress hormone level and oxidative damage index in the blood of the sow under the heat stress condition, improve the activity of antioxidant enzyme, improve the feed intake and lactation performance in the lactation period, and reduce the backfat loss. Meanwhile, through the vertical transfer effect of the sows and the piglets, the intestinal morphology of the offspring piglets is remarkably improved, the intestinal barrier function and the antioxidant capacity are enhanced, and the weaning weight and the average daily gain of the piglets are improved, so that the negative effects of heat stress on the reproductive performance of the sows and the growth of the piglets are comprehensively relieved. The preparation is green and safe, and has important application value.

The invention belongs to the technical field of biomedicine, and particularly relates to a preparation method and application of baicalein supramolecular nanobelts.The baicalein supramolecular nanobelt for relieving intestinal injury of crisp tilapia mossambica is prepared according to the following steps that baicalein and dimethyl sulfoxide are mixed, then water is added for stirring, then centrifugal cleaning and dialysis are conducted with purified water, and the baicalein supramolecular nanobeltssambica is obtained; and freezing and drying to obtain the baicalein supermolecule nanobelt. The water solubility and bioavailability of the baicalein supramolecular nanobelt are remarkably improved, the crisp meat quality of tilapia can be kept, and intestinal inflammation can be relieved.

The invention discloses a compound traditional Chinese medicine and application thereof in preparing a medicine for resisting eimeria tenella or treating coccidiosis, and relates to the technical field of livestock breeding. The compound traditional Chinese medicine comprises the following raw materials in parts by mass: 5-20 parts of hesperidin and 1-10 parts of citric acid. According to the invention, chicks are taken as experimental subjects, a chick coccidiosis model is established, and the effect of the compound traditional Chinese medicine on the chick coccidiosis is researched from the aspects of general conditions of the chicks, bloody stool conditions, coccidiocyst discharge conditions, cecum tissue lesion conditions, pathological detection, serum biochemistry, oxidation resistance detection and the like. The compound traditional Chinese medicine achieves a good anti-coccidiosis drug level, the required raw materials are low in cost, convenient to use and free of hormones and antibiotics, no toxic or side effect is caused to chickens, drug resistance cannot be caused, a new choice is provided for replacing chemical drugs for preventing and treating coccidiosis, and addition of anti-coccidiosis drugs in chicken feed is expected to be reduced or replaced.

The utility model relates to livestock feeding ventilation technical field especially is a kind of livestock feeding ventilation device, including the ventilation pipe of being inserted with the feeding house wall body, the upper end and the lower end of ventilation pipe are all fixedly connected with the feeding house wall body fixed connection's one triangle reinforcing steel and two triangle reinforcing steels, one end fixedly connected with the fan cover in the ventilation pipe located in feeding house, detachably installed in ventilation pipe has filter assembly, four no.

The application provides application of a TGBp1 gene, or a protein coded by the TGBp1 gene, or a recombinant carrier or host bacteria containing a coding region of the TGBp1 gene in down-regulating expression level of MeGRXC3. The application research finds that there is interaction between TGBp1 and MeGRXC3, the growth ability of yeast in SD / -Leu / -Trp / -His / -Ade auxotrophic medium can be improved, the expression of MeGRXC3 can be down-regulated, the active oxygen content of tobacco leaves co-expressing TGBp1 and MeGRXC3 is improved, and the growth ability of yeast in SD / -Leu / -Trp / -His / -Ade auxotrophic medium is improved. The research result lays a theoretical foundation for cassava common mosaic virus disease-resistant breeding.

The utility model belongs to the technical field of compound microbial fertilizer, and particularly relates to environment-friendly compound microbial fertilizer premixing equipment which comprises a bottom plate, fixing plates are mounted on two sides of the upper end of the bottom plate, a top plate is mounted at the upper ends of the two fixing plates jointly, and sliding grooves are formed in the ends, close to each other, of the two fixing plates. And sliding blocks are slidably connected into the two sliding grooves, a mounting plate is jointly mounted between the two sliding blocks, and a mixing assembly is arranged on the mounting plate. Through the arrangement of the mixing assembly, the microbial fertilizer can be uniformly mixed, blockage generated during discharging can be avoided, and the discharging effect and the stirring uniformity are guaranteed; by arranging the adjusting assembly, the height of the mounting plate can be adjusted, and then the stirring position is adjusted, so that the microbial fertilizers with different depths in the mixing barrel can be stirred and mixed, the mixing effect is greatly improved, the soil fertility and the crop growth effect can be remarkably improved, and the crop quality is met.