22results about How to "Improve growth performance" patented technology

This invention relates to the field of plant genetic engineering technology, and more particularly to... SsNHX1 and SsSOS2 Dual-gene plant expression vector and its application in improving salt tolerance in alfalfa. The plant expression vector uses pCAMBIA1300 as its backbone and inserts genes derived from Suaeda salsa. SsNHX1 Genes and SsSOS2 It is constructed from genes. SsNHX1 Genes and SsSOS2 The CDS coding sequence of the gene is shown in SEQ ID NO:1 and SEQ ID NO:2 of the sequence listing. Using Agrobacterium tumefaciens EHA105-mediated transformation, the vector was introduced into the leaves of sterile alfalfa seedlings. Positive transgenic plants were obtained through co-culture, callus induction differentiation, and PCR identification. The advantage lies in the synergistic effect of the two genes, which effectively enhances the salt tolerance of alfalfa, enabling it to grow normally under 200–300 mM NaCl stress, providing a new solution for forage cultivation in saline soils.

The application belongs to the technical field of biology and particularly relates to a molecular marker for predicting daily weight gain of pigs, a primer pair, a kit and application thereof. The nucleotide sequence of the molecular marker is shown as SEQ ID NO. 3, and the 474th site at the 5' end of the molecular marker is a polymorphic site, the base of which is C or G, and the daily weight gain of the pig with the genotype CC of the site is higher than that of the pig with the genotype GG. The molecular marker provided in the application is a molecular marker related to the growth performance of pigs, and is used for predicting the daily weight gain, feed conversion rate and other growth traits of pigs, and provides an accurate genetic basis for early selection of pigs.

The application discloses a kind of fermented feed fermented by three strains of probiotics of Bacillus YA ( Bacillus sp ), yeast Y ( Saccharomyces cerevisiae ) and capsular red bacteria R3 ( Rhodobacter capsulatus ), and preparation method and application in aquaculture thereof, and viable count is not less than 1×10 7 CFU / g.The fermented feed is replaced ordinary feed according to certain proportion, and feeding result shows that aquaculture water quality is significantly improved after adding fermented feed, and fish growth performance, digestive enzyme activity, immunity, and muscle quality are also obviously improved.Using the feed in suitable range can shorten growth cycle, reduce cost, improve aquaculture environment, reduce antibiotic use, and improve muscle quality of farmed animals, with good economic and ecological benefits, simple fermentation process, low cost, and suitable for industrial large-scale production.

The application discloses a process for improving the quality of palm kernel meal feed by enzyme and bacteria synergistic fermentation, and belongs to the field of feed biotechnology.The process is characterized by the following steps: crushing and screening palm kernel meal, mixing the palm kernel meal with 20% bran and 4% ammonium sulfate as dry matter to obtain a basic substrate;compounding bacillus subtilis, aspergillus niger and saccharomyces cerevisiae at a ratio of 1:1:1 of viable bacterial number, adding 0.67% mannanase and 0.3% xylanase to prepare a bacteria-enzyme mixed solution, inoculating the bacteria-enzyme mixed solution at a 10% inoculation amount, adjusting the moisture content to 50% and the pH to 6.4, and then sealing and storing the bacteria-enzyme mixed solution for use after 4 days of fermentation at 33.5 DEG C; and the bacteria-enzyme mixed solution can be stored for a long time.The process can efficiently degrade crude fiber and anti-nutritional factors, improve the protein content and the digestibility, is stable and easy to mass-produce, can replace part of corn and soybean meal in the daily ration of ruminants, and realizes high-value utilization of palm kernel meal.

The application discloses a kind of fulvic acid hydrogen peptide biological stimulant hormone fertilizer and preparation method thereof, belong to fertilizer technical field.Fertilizer is by 15~30 parts of fulvic acid, 5~12 parts of peony seed meal small molecule peptide, 20~35 parts of potassium dihydrogen phosphate and other components compound, water is made up to 100 parts, and auxiliary agent is emulsifier and chelating agent compound.Preparation first by dissolving fulvic acid, potassium phosphate in water and obtaining mixed solution, then adding small molecule peptide and auxiliary agent and stirring until the system is stable;Small molecule peptide is prepared by peony seed meal defatted powder crushing and enzymolysis, solves the problem of traditional fertilizer nutrient antagonism, unstable system, and the utilization of peony seed meal also improves resource utilization.Tomato, cucumber seedling test shows that it can significantly improve the growth index of crop root system and aboveground part, and the root-promoting and seedling-strengthening effect is better than that of similar fertilizer on the market, and the preparation process is simple, easy to industrialization, and has good agricultural application prospect.

This invention provides a drug for antagonizing bacterial endotoxins, its preparation method, and its application, belonging to the biomedical field. The composition consists of 25-55 parts of dried powdered traditional Chinese medicine extract, 25-70 parts of mixed essential oil powder, and 40-90 parts of lentinan powder. The traditional Chinese medicine extract is prepared by decoction of Scutellaria baicalensis, Lonicera japonica, Forsythia suspensa, and Isatis indigotica under reduced pressure at low temperature and spray drying. The mixed essential oil powder is prepared by encapsulating peppermint oil and eucalyptus oil with gelatin and hydroxypropyl cellulose and then spray drying under high pressure. This invention, through specific formulation and processing, significantly enhances the synergistic antagonistic effect of each component against endotoxins. Experiments show that this composition exhibits antagonistic activity far superior to ibuprofen in vitro; in vivo, it significantly reduces the endotoxin content in the plasma of 817 broiler chickens, rapidly alleviates LPS-induced fever and diarrhea, and improves decreased growth performance. Therefore, this invention is of great significance for reducing antibiotic dependence and improving the efficiency of livestock and poultry farming.

This invention relates to recombinant mussel adhesive proteins, the nucleic acid molecules encoding them, nucleic acid constructs, expression vectors, recombinant engineered strains, pharmaceutical compositions, preparation methods, purification methods, and applications. This invention assembles and splices partial functional sequences from different mussels and type III collagen to obtain several novel mussel adhesive protein sequences. Further screening yielded recombinant mussel adhesive proteins Mfp5, Mfp55, Mfp131, and Mfp151, suitable for large-scale industrial production and exhibiting high expression levels. Efficacy experiments demonstrated that these proteins significantly promote cell migration, indicating their excellent cell repair function and broad application prospects.

This invention provides the application of polyferric sulfate in improving plant salt stress resistance and / or improving saline-alkali land, belonging to the field of agricultural technology. The polyferric sulfate application provided by this invention can effectively improve the germination ability of plant seeds under salt stress, effectively enhance the activity of plant antioxidant systems and photosynthetic efficiency, thereby effectively improving the survival rate and growth performance of plants under salt stress. Simultaneously, polyferric sulfate can effectively reduce the EC value of saline-alkali soil, thereby effectively improving the germination rate of plant seeds in saline-alkali land and effectively improving saline-alkali land. This invention is simple to operate, low in cost, and environmentally friendly, suitable for enhancing the salt and alkali tolerance of plants, and has broad application prospects.

This invention provides a *Roseolariomoraxella salina* strain for saline-alkali soil improvement, with accession number CGMCC No. 36067. The *Roseolariomoraxella salina* strain provided by this invention exhibits significant comprehensive application advantages. This strain demonstrates excellent survival and growth capabilities under saline-alkali stress, effectively reducing salinity and alkalinity in saline-alkali soils and thus improving the saline-alkali environment. Whole-genome analysis shows that this strain carries multiple functional genes related to osmotic regulation and ion transport, providing a molecular basis for its salinity-reducing ability. Adding citric acid or betaine can further enhance its salinity-reducing performance, and its biomass is significantly increased after optimization of fermentation conditions. This strain shows promising application prospects in saline-alkali soil improvement and saline-alkali aquaculture environment management.

The utility model relates to livestock feeding ventilation technical field especially is a kind of livestock feeding ventilation device, including the ventilation pipe of being inserted with the feeding house wall body, the upper end and the lower end of ventilation pipe are all fixedly connected with the feeding house wall body fixed connection's one triangle reinforcing steel and two triangle reinforcing steels, one end fixedly connected with the fan cover in the ventilation pipe located in feeding house, detachably installed in ventilation pipe has filter assembly, four no.

This invention relates to an enzymatically hydrolyzed protein composition as a substitute for fishmeal and its preparation method, belonging to the field of feed technology. The enzymatically hydrolyzed protein composition comprises the following raw materials: animal protease hydrolysate, brewer's yeast extract, bile acids, sodium butyrate, sodium selenite, and fructooligosaccharides. This application selects cricket powder and defatted mealworm powder as animal protein sources. Simultaneously, chitinase, alkaline protease, and neutral protease are used sequentially to enzymatically hydrolyze the cricket and mealworm complex. The cricket powder and defatted mealworm powder after enzymatic hydrolysis effectively eliminate anti-nutritional factors in the original insect feed, and have high protein utilization, effectively releasing nutrients and improving nutritional value. Based on this, brewer's yeast extract and bile acids are added for compounding, which synergistically promotes weight gain and growth in aquatic animals. Furthermore, this application uses sodium butyrate, sodium selenite, and fructooligosaccharides in combination, which effectively gives the prepared composition significant antioxidant effects from different dimensions.

The present application relates to the technical field of feed engineering and microbial application, and discloses a probiotic chicken feed and a preparation method thereof.The feed comprises corn flour, soybean meal, wheat bran, a synergistically modified probiotic carrier, natural plant enzyme and various probiotics, wherein the probiotics comprise Lactobacillus delbrueckii subsp.Bulgaricus, Bacillus subtilis, yeast and rice koji.The synergistically modified probiotic carrier is formed into a limited inclusion and ion crosslinking structure by β-cyclodextrin and chitosan in the presence of calcium lactate, and is used to coat and modify the probiotics and natural plant enzyme.Through the above structure design, the stability of the probiotics and enzyme during processing and storage can be effectively improved, and the survival ability in the gastrointestinal environment and the intestinal colonization effect can be enhanced, so as to improve the feed utilization rate and improve the growth performance of chickens.The present application has simple process, is suitable for large-scale production, and has good application prospect.

The application provides a sodium dimethylate feed additive for improving growth performance of broilers and an application thereof, and relates to the technical field of livestock and poultry feed additives, and is prepared from the following raw materials: sodium dimethylate, compound probiotics, organic acid calcium salt, amino acid chelate, mixed vitamins, natural plant extract and carrier; the sodium dimethylate in the application reduces the pH value of the intestinal tract and inhibits harmful bacteria; the compound probiotics regulate the balance of intestinal flora and promote the growth of beneficial bacteria; the organic acid calcium salt provides a calcium source and enhances the antibacterial effect; the amino acid chelate supplements necessary amino acids and improves the protein utilization rate; the mixed vitamins enhance the immunity and stress resistance of the body; through the synergistic action of multiple components, the growth performance of the broilers is significantly improved, and the needs of the broilers in the whole growth stage are met.

This invention discloses a biological agent suitable for squab growth, its preparation method, and its application, belonging to the field of feed technology. It comprises the following components by weight: 8-40 parts of microbial agent, 1-10 parts of additive, 1-10 parts of antioxidant, 0.5-5 parts of peptidoglycan, and 40-60 parts of montmorillonite. The microbial agent includes *Bifidobacterium animalis*, *Lactobacillus acidophilus*, *Lactobacillus paracasei*, and *Lactobacillus reuteri*. The additive includes at least one of yeast β-glucan and astragalus polysaccharide. The biological agent for squab growth of this application uses montmorillonite as a base material. Under the combined action of the microbial agent, additive, antioxidant, and peptidoglycan, it can regulate the intestinal health of breeding pigeons, improve their immunity, and increase immune factors in pigeon milk. This can improve the growth performance of squabs, reduce the culling rate of both breeding and squabs, thereby increasing economic benefits and showing broad application prospects.

This invention belongs to the field of feed additive technology, specifically relating to a microalgae compound feed additive for alleviating weaning stress in calves and its preparation method. The microalgae compound feed additive comprises the following raw materials in parts by weight: 5-10 parts microalgae powder, 2-6 parts unsaturated fatty acid / modified montmorillonite complex, 0.7-1.2 parts probiotics, and 0.3-0.5 parts walnut shell extract. This microalgae compound feed additive can improve the fragile intestinal barrier function and immunity of weaned calves, enhance their growth performance, and thus improve breeding efficiency.

This invention relates to the field of probiotic development and utilization technology, specifically to a probiotic strain of *Lactobacillus plantarum* SWUN3927 and its applications. The *Lactobacillus plantarum* (… Lactobacillus plants SWUN3927, isolated from colostrum of Tibetan yaks, was used in this invention to identify the probiotic effects of *Lactobacillus plantarum* SWUN3927 through animal experiments. The results showed that *Lactobacillus plantarum* SWUN3927 is resistant to acid and bile salts and has an inhibitory effect on pathogenic bacteria; its hydrophobicity is 74.67% and its self-aggregation is 58.98%, demonstrating that *Lactobacillus plantarum* SWUN3927 of this invention has good intestinal adhesion and possesses the potential of a probiotic. This invention verifies that *Lactobacillus plantarum* SWUN3927 can effectively regulate animal immunity, improve the body's antioxidant capacity, increase lysozyme content and alkaline phosphatase activity, improve daily weight gain, and ultimately improve animal growth performance.

The utility model discloses a full-automatic livestock raising shed which comprises a wall body, a plurality of compartments are fixedly connected to the outer wall of the bottom of the wall body, a first filtering assembly is arranged on the outer wall of the bottom of each compartment and comprises a water tank fixed to the outer wall of the bottom of each compartment, and a filtering net and a granular medium filtering net are arranged in each water tank. The bottom of the water tank is in bolted connection with a water conveying pipe, and a first water pump is arranged at one end of the water conveying pipe. A second filtering assembly is arranged on one side of the wall body and comprises a box body, filtering sponge and activated carbon are arranged in the box body, and the filtering sponge is located above the activated carbon. The feeding shed is provided with the first filtering assembly which can preliminarily filter sewage in the compartments, the second filtering assembly can further adsorb tiny impurities and peculiar smell through the filtering sponge and the activated carbon in the box body, and the temperature in the shed is effectively reduced through the heat dissipation assembly.

A method for collecting blood and preparing serum from giant freshwater prawns includes: feeding the prawns a nutrient-rich diet under low-temperature conditions, collecting blood from their second walking legs, and obtaining purified serum through blood coagulation, serum precipitation, and further processing; the nutrient diet includes at least a protocatechuic acid-chitosan oligosaccharide derivative, which is obtained by grafting protocatechuic acid onto chitosan oligosaccharide. This invention preserves the life of individual giant freshwater prawns during blood collection and increases the amount of blood and serum collected.

This invention relates to the field of agricultural technology, and particularly to the application of zinc citrate in improving plant salt stress resistance and remediating saline-alkali land. This invention is the first to utilize zinc citrate to enhance plant salt stress resistance. Zinc citrate can effectively improve the germination ability of plant seeds under salt stress, increase the activity of the plant's antioxidant system and photosynthetic efficiency, thereby effectively improving the survival rate and growth performance of plants under salt stress. Simultaneously, zinc citrate can effectively reduce the EC value of saline-alkali soil, achieving effective remediation of saline-alkali land, and further improving the germination rate of plant seeds in saline-alkali land.

The application discloses a kind of recombinant lactococcus solid fermentation medium, fermentation and its application.The medium provided by the application comprises the following components by weight: 85~93 parts of soybean meal, 2~5 parts of glucose, 1~2 parts of calcium carbonate, 2~4 parts of radix astragali, 1~3 parts of atractylodes, 1 part of sanguisorba officinalis.The application also discloses a method for preparing recombinant lactococcus solid fermentation using the medium, and the prepared solid fermentation.The experiments verify that the atractylodes and sanguisorba officinalis added in the medium of the application can synergistically promote the proliferation of lactococcus, significantly increase the solid fermentation viable count;The obtained recombinant lactococcus solid fermentation can increase the level of intestinal mucosa sIgA, significantly reduce the cecal lesion score of coccidiosis infected chicken, reduce the amount of oocyst discharge, and improve growth performance.The application provides a new technical solution for the green and safe prevention and control of coccidiosis, and has good application prospect.

This utility model discloses a precision cultivation device for plant tissue culture, comprising a liquid tank, a motor fixedly connected to the left surface of the liquid tank, and a first reciprocating screw fixedly connected to the output end of the motor. By setting up a structure including a motor, a first reciprocating screw, a first conical tooth, a second reciprocating screw, a second conical tooth, a fixed base, a transmission shaft, a third conical tooth, and a fourth conical tooth, the entire cultivation rack can be moved left and right, ensuring that the plants in each cultivation tank are evenly sprayed with the nutrient solution, thereby improving the overall growth effect of the plant group. By setting up a fixed base, a rotating shaft, gears, stirring blades, and a rack, the stirring blades can be rotated when the cultivation rack moves left and right, thus fully stirring the nutrient solution inside the liquid tank. This prevents sedimentation and clumping in the nutrient solution, maintaining its fluidity and ensuring that the plants can continuously absorb fresh nutrients.

The present application relates to the technical field of microbial engineering, in particular to modified corynebacterium microorganism and its construction method and application. The present application provides the application of weakening or inactivating non-essential genes in improving the threonine yield of microorganism or constructing threonine-producing microorganism; the non-essential genes are cg1507-cg1524, Cgl1336-Cgl1352 or NCgl1281-NCgl1298. The present application significantly improves the threonine production capacity of the strain by inactivating the non-essential genes, and improves the growth performance of the strain. The modification of the above non-essential genes can be used in the fermentation production of threonine, and has good application value.