212 results about "Hybrid seed" patented technology

A Chinese cabbage hybrid seed production marking method relates to the technical field of Chinese cabbage hybrid seed production. The technical scheme of the method is as follows: after Chinese cabbage hybrid seed production pollination is finished, firstly holding the pedicel of a pollinated flower with the left hand; then transversely tearing off two sepals from the root with the right hand, wherein light movement is required. The Chinese cabbage hybrid seed production marking method can perform marking without rope tying, ring arranging or stamp-pad ink coating, and is low in cost and high in accuracy.

The invention discloses an acer elegans cross breeding method based on flowering phase difference regulation and control, and belongs to the technical field of garden plant breeding. The method comprises the following steps: selecting an acer elegans variety of which the natural early flowering phase difference is greater than or equal to 10 days and the genetic distance is greater than or equal to 0.5 as a cross parent, carrying out low-temperature refrigeration and shading blue light supplementing treatment on a female parent to delay the flowering phase, and carrying out prolonged illumination, red light supplementing and hormone and nutrient solution spraying on a male parent to advance the flowering phase; male parent pollen is collected through a vacuum negative pressure adsorption device and stored in a closed container, a sucrose solution is sprayed to female parent stigmas before pollination, and repeated pollination is conducted through a directional nozzle at 9-11 o'clock every day; carrying out stratification treatment and temperature-difference temperature-control germination on the hybrid seeds, and carrying out light-temperature regulation and control seedling culture after sowing; the molecular marker is used for verifying the hybridization authenticity and target traits, phenotype determination is carried out on three-year-old seedlings to complete breeding, the hybridization obstacle caused by asynchronous flowering phases of the caenorhabditis elegans varieties is solved through the flowering phase regulation and control technology, and the hybridization success rate is increased.

A method is provided for determining soil properties for an area of land, from soil spectrum data. In an embodiment, the method includes receiving soil spectrum data records from hyperspectral sensors that represent a mean soil spectrum of a specific geo-location of the area of land and removing interference signals from the spectrum data records to create soil spectral bands. The method also includes predicting a plurality of soil property datasets based on a partial least-square regression and the soil spectral bands and selecting specific soil property datasets from the plurality of soil property datasets to represent soil properties of the specific geo-location, wherein the specific soil property datasets include property data and spectral band data for spectral bands used to determine the property data. The specific soil property datasets may then be used to generate a crop prescription of recommended hybrid seeds or population densities for the specific geo-location.

The invention discloses a male sterility gene ZmUCE4 and application thereof in creating a male sterility line of corn, and belongs to the field of plant biotechnology breeding. According to the invention, site-directed mutagenesis of the ZmUCE4 gene in wild type corn is carried out through a CRISPR / Cas9 gene editing technology, pollen development abnormality and abortion can be caused, complete male sterility is caused, and it is proved that the ZmUCE4 gene has a regulation and control function on corn male reproductive development. Through offspring screening, a sterile line without transgenic components can be obtained, a stable maize male sterile line is created, and the method has important significance on maize male fertility control and hybrid seed production. The invention also designs a functional molecular marker aiming at the obtained uce4 male sterility mutant gene, and has important application value in maize male sterility line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of a GhDCP5L gene in creation of high-temperature response cotton germplasm, and an amino acid sequence of a protein coded by the GhDCP5L or a homologous gene thereof is as shown in SEQ ID NO.2 or SEQ ID NO.4. The invention provides a novel thermal response gene, a gene resource library of high-temperature response of cotton is enriched, the GhDCP5L gene and a homologous gene thereof are applied to plant genetic engineering breeding, and new germplasm of high-temperature-resistant or sensitive high-temperature-resistant cotton can be accurately created by regulating and controlling the expression of the gene, so that the high-temperature-resistant or sensitive high-temperature-resistant cotton is obtained. The method provides technical support for coping with stable yield of cotton under the background of global warming climate, can be used for preparing male sterile line cotton germplasm by performing high-temperature treatment on the high-temperature-sensitive material, has good application value in the aspects of cotton hybrid seed production, promotion of hybrid cotton development and the like, and is beneficial to improving the economic benefit of the cotton industry.

The invention relates to a gene ZmILR1 for controlling the size of a corn kernel as well as a molecular identification method and application of the gene ZmILR1, and belongs to the technical field of plant genetic breeding and functional gene research. The ZmILR1 gene encodes an IAA-amino acid hydrolase, mutation of the IAA-amino acid hydrolase causes corn kernels to become smaller, and overexpression of the gene can create a corn material with increased kernel length, kernel width and hundred-kernel weight. A functional molecular marker A1A4 is developed according to the sequence difference of the mutant gene and a wild type gene at the DNA level, and can be used for rapid identification and screening of small-grain corn materials. The invention provides a theoretical basis and an application tool for analyzing a molecular mechanism of the corn grain size and developing molecular marker-assisted breeding and hybrid seed breeding, and provides important gene resources and technical support for high-yield and high-quality molecular breeding of corn.

An example seed structure, systems, and methods for conducting crystal growth processes are provided. In one example, the present disclosure provides an example seed structure for a silicon carbide crystal growth system. The seed structure includes a carrier layer. The carrier layer is silicon carbide. The seed structure includes a seed layer bonded to the carrier layer with a bond. The seed layer is crystalline silicon carbide. The seed layer provides a growth surface for growing a crystalline silicon carbide structure in a silicon carbide crystal growth process.

The invention belongs to the field of molecular markers and detection thereof, and particularly relates to an SNP (Single Nucleotide Polymorphism) site primer combination for identifying the authenticity of tomato varieties and the purity of hybrids and application of the SNP site primer combination. The three SNP primer combinations provided by the invention can be respectively used for performing early identification on a common tomato variety, a cherry tomato variety and a taste tomato variety in a seed or seedling stage, so that the authenticity of the varieties and the purity of hybrids are ensured, and meanwhile, whether two or more unknown tomato varieties belong to the same variety or not can be identified; and the hybrid purity of the tomato variety to be detected. The rights and interests of producers and breeders can be practically protected, and technical support is provided for protection of tomato germplasm resources and new varieties.

The present invention relates to diploid, fertile, self-compatible and essentially homozygous potato lines, wherein said lines comprise an agronomically desirable trait such as vigour. The invention further relates to methods for producing such plants and to hybrid seeds obtained by crossing such homozygous potato lines and to potato plants grown from said seed.

The present invention relates to diploid, fertile, self-compatible and essentially homozygous potato lines, wherein said lines comprise an agronomically desirable trait such as vigour. The invention further relates to methods for producing such plants and to hybrid seeds obtained by crossing such homozygous potato lines and to potato plants grown from said see.

The present application provides an isolation, cloning, functional verification of a restorer gene OsRf19 for cytoplasmic male sterility in rice and its use in improving rice. The present application specifically provides a nucleotide sequence of the gene OsRf19 and an encoded amino acid sequence thereof, an expression vector comprising the nucleotide sequence of the gene OsRf19, a method of creating a restorer line of rice using the gene OsRf19, and a method of restoring the fertility of a cytoplasmic male sterility line of rice and producing rice hybrid seeds, and related uses.

The invention belongs to the field of plant biotechnology breeding, and relates to an application of a ZmGalAT1 gene and an encoding protein thereof in corn fertility control. According to the invention, a corn male sterility mutant galat1 is obtained by EMS mutagenesis, and a gene ZmGalAT1 for controlling corn male flower development is identified through map-based cloning. The gene is subjected to site-directed mutagenesis in wild type corn through a CRISPR / Cas9 gene editing technology, three galat1 allelic corn male sterility mutants are created, corn male flowers of the mutants are completely aborted, sterile mutants without transgenic ingredients can be obtained through offspring screening, and the mutants are further applied to corn sterile breeding and seed production. The invention also designs functional molecular markers for three allelic male sterile mutants of GalAT1, and has important application value in maize male sterile line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The present invention relates to hybrid barley seed production and marker assisted selection of barley plants in order to obtain barley plants or seeds comprising an Ant 1 and / or Ant2 gene allowing color sorting of seeds.

The application discloses InDel marker primers for identifying authenticity or seed purity of a cauliflower variety Jinpin 81 and a method for identifying the same. The application screens and designs specific InDel sites and selects five pairs of InDel marker primers from the specific InDel sites by analyzing parent resequencing data of the cauliflower variety 'Jinpin 81' in a whole genome range, and the nucleotide sequences of the five pairs of InDel marker primers are respectively shown in SEQ ID NO. 1-SEQ ID NO. 10. The identification method established by applying the five pairs of marker primers can quickly and accurately complete identification of authenticity of the cauliflower variety 'Jinpin 81' and purity of hybrid seeds thereof, solves problems of long cycle, high cost and low accuracy existing in traditional identification methods, has advantages of high identification accuracy, simple and fast operation, small sample requirement and the like, greatly improves identification efficiency and accuracy, and effectively guarantees seed production quality of the cauliflower variety 'Jinpin 81'.

The invention provides a recombinant TKC vector applied to CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated 9) and a construction method of a mutant plant, and belongs to the technical field of gene editing. The invention provides a recombinant TKC vector applied to CRISPR-Cas9, the recombinant TKC vector comprises a U6 transcription box and / or a U3 transcription box, a target gene in the transcription box is located between a promoter and a terminator, and transcription expression of the target gene can be effectively guaranteed. By using the recombinant TKC vector and knocking out the EMF1 gene in a japonica rice dry-hui 89 material, the daily flowering time of the japonica rice dry-hui 89 material is obviously earlier, the flowering time of hybrid seed production of indica rice and japonica rice is encountered, the yield of hybrid seed production of indica rice and japonica rice is favorably improved, and the seed production cost is reduced. By utilizing the gene editing method provided by the invention, the daily flowering time of the japonica rice variety, i.e., the Yanhui 89 can be obviously advanced, and the problem of infertility during flowering during indica-japonica hybrid seed production is solved.

The invention belongs to the technical field of cassava planting, and discloses a method for improving the germination rate and transplanting survival rate of cassava hybrid seeds, and the method comprises the following steps: S1, soaking the cassava hybrid seeds in a potassium permanganate solution, and then soaking in a glucose solution; s2, weighing the garden soil, the fine sand and the fermented sugarcane filter mud in proportion; s3, sowing the pretreated seeds on a seedling culture substrate; s4, placing the seedling raising tray in an environment with the temperature of 25-30 DEG C, the humidity of 70%-80% and the illumination intensity of 3000-5000 lux for accelerating germination, and when the germination rate reaches 80%, moving the seedling raising tray out for transplanting; s5, the roots of the seedlings are soaked in a rooting powder solution, then the seedlings are transplanted into a field or a nutrition bowl prepared in advance, and a sunshade net is used for sunshade treatment 3 days before transplanting. The problems that existing cassava hybrid seeds are low in germination rate and low in seedling transplanting survival rate are solved.

The utility model discloses efficient hybrid seed cultivation equipment in a corn variety breeding process, and belongs to the technical field of corn hybrid seed cultivation. The efficient hybrid seed cultivation equipment in the corn variety breeding process comprises a cultivation box and further comprises a fixing frame, the fixing frame is located in the cultivation box, a plurality of cultivation boxes are arranged in the fixing frame, and a water tank is arranged at the upper end of the cultivation box. In order to solve the problems that according to an existing cultivation box, it is inconvenient for personnel to observe and evaluate corn seedlings, the corn seedlings are prone to mutual shielding, and consequently seedling character evaluation is difficult to conduct, cultivation boxes are installed in a fixing frame, the multiple cultivation boxes are distributed in a step shape, and the corn seedlings are located in the cultivation boxes; due to the fact that the height difference exists between the two longitudinal cultivation boxes and the two transverse cultivation boxes are separated, a larger growth space is provided for corn seedlings, meanwhile, personnel can observe and evaluate the seedlings conveniently, and the breeding efficiency is improved.

The invention relates to the technical field of molecular identification of muskmelon hybrid seed purity, discloses an SSR primer and a method for rapidly identifying muskmelon hybrid seed purity, and aims to solve the problems that traditional seed purity detection depends on field test and manual observation, the detection period is long, the cost is high and the like. The invention provides two SSR primers with high polymorphism and good specificity for the melon hybrid 'Suqian No.4' and the specific markers thereof, and the SSR primers are applied to purity identification of 'Suqian No.4' seeds, so that good technical support is provided for seed production and seed sales, and the result is reliable. The method has the advantages of rapidness, accuracy, low cost, good repeatability, strong operability and the like.

The present disclosure provides methods for increasing yield in a hybrid seed production field and for allocating resources for hybrid seed production. In certain aspects, the methods for increasing yield in a hybrid seed production field utilize learned prediction models to determine optimal planting dates or management practices during plant growth and development.

The invention relates to a pepper promoter CanASP28 and application thereof, and belongs to the technical field of gene engineering. The nucleotide sequence of the pepper promoter CanASP28 is shown as SEQ ID NO.5, and the pepper promoter CanASP28 can drive specific expression of anther in plants, can be used for creating male sterile lines, crop hybrid seed production and crop crossbreeding, can improve the seed purity and hybrid seed production and crossbreeding efficiency, and can be applied to the field of plant breeding. Therefore, an effective tool gene is provided for anther specific expression regulation and pollen expression regulation of pepper and other solanaceae crops.

This invention relates to the field of plant variety breeding technology, and in particular to a method for improving the hybrid seed setting rate of rice under high temperature conditions. The technical solution is as follows: A method for improving the hybrid seed setting rate of rice under high temperature conditions includes an operational procedure for improving the hybrid seed setting rate of rice under high temperature conditions. This invention transfers the pollination process indoors and uses an air conditioning system to maintain a stable temperature within a suitable range. Simultaneously, it utilizes hydroponics to provide a continuous and stable root moisture and local humidity environment for the pollinated plants, thereby creating a controlled microclimate. This not only avoids pollen inactivation and stigma drying caused by high temperature and dryness, but also prevents fertilization obstacles caused by drastic humidity changes, allowing pollen and stigma to successfully complete the pollination and fertilization process in a continuously suitable environment, thus effectively improving the hybrid seed setting rate.

The application discloses a preparation method of a hybrid seed layer assisted growth perovskite film and a perovskite-crystalline silicon laminated solar cell, and belongs to the technical field of solar cells. The perovskite film preparation method provided by the application comprises the following steps: (1) wetly depositing a hybrid seed layer solution on the surface of a substrate, and cooling after heat treatment to obtain a hybrid seed layer; and (2) dryly depositing an inorganic metal salt on the surface of the hybrid seed layer to form an inorganic metal salt layer, and then performing annealing treatment to obtain a perovskite film. The hybrid seed layer has the functions of crystal nucleus induction activity and interface regulation, a stable solid-solid reaction interface is constructed between the substrate and the precursor layer through a low-temperature liquid phase pre-deposition process, and bidirectional interface passivation, crystallization orientation regulation and energy level optimization are simultaneously realized, so that the photoelectric conversion efficiency and long-term operation stability of the laminated cell are significantly improved, and excellent batch preparation repeatability is achieved.

The invention discloses a smut-resistant sugarcane hybrid variety breeding method. The smut-resistant sugarcane hybrid variety breeding method comprises the specific steps that sugarcane hybrid seeds are cultivated into seedlings; inoculating the seedlings with smut fungus spores for disease resistance primary screening; then the plants are further screened through a double-track smut resistance test, and then screening of the high-quality sugarcane is achieved. According to the breeding method, the efficiency of breeding the high-quality smut-resistant sugarcane variety is improved, test errors caused by spore activity differences can be reduced, the test stability is improved, the smut resistance of ratoon sugarcane and newly-planted sugarcane is respectively tested by adopting a double-track smut-resistant test, and the breeding efficiency of the high-quality smut-resistant sugarcane variety is improved. Meanwhile, the influence of inoculation of smut fungi by adopting a spraying method and a dipping method on sugarcane screening can be observed, and the efficiency and comprehensiveness of breeding the smut-resistant sugarcane are improved.

This invention relates to the field of shape memory material preparation technology, specifically to a bio-based shape memory Eucommia ulmoides gum composite material and its preparation method. All components of the composite material are of entirely bio-based origin, using natural Eucommia ulmoides gum as the matrix. Nano-hybrid seed crystals composed of cellulose nanocrystals and carbon quantum dots are introduced. The cellulose nanocrystals act as nucleating agents, guiding and accelerating the cooling crystallization of the Eucommia ulmoides gum, while the carbon quantum dots form the shell, improving heat transfer efficiency. In the preparation process, this application uses triacylglycerol lipase as a biocatalyst, which can initiate an in-situ esterification reaction between the filler and the matrix at a low temperature of 65-75℃. This not only reduces energy consumption but also avoids the thermal degradation and damage to the Eucommia ulmoides gum macromolecular chains caused by high-temperature strong shear, achieving high performance and batch-to-batch stability of the material.

The invention provides a method for creating a tomato long stigma type male sterile line through gene editing. The method comprises the following steps: step S1, inserting a 450bp nucleotide sequence as shown in SEQ ID NO.1 into a Style2.1 gene promoter region; step S2, knocking out the SlLAP3 gene in the genome of the tomato material by utilizing a CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats / associated protein 9) system; s3, introducing the recombinant plasmids meeting the requirements of the step S1 and the step S2 into a tomato material to obtain the tomato long stigma type male sterile line. The tomato strain of the long stigma type male sterile line is obtained, is normal in growth and development, exposed in stigma and abortive in pollen, can normally bear fruits after being hybridized with a wild tomato plant, provides a practical technology and a new male sterile resource for tomato cross breeding, and has a very good application prospect in the aspect of tomato hybrid seed production.

The application discloses a KASP molecular marker closely linked with pepper stem tip color, takes a zhangshugang version gene as a reference gene, and the KASP molecular marker is at 148515563 of a pepper 10th chromosome, a single nucleotide polymorphism at the position, and a base C to A replacement occurs at the position. The application further discloses primers, a kit for identifying the KASP molecular marker closely linked with the pepper stem tip color, and application of the KASP molecular marker in identifying the pepper stem tip color or identifying purity of pepper seeds. The KASP molecular marker closely linked with the pepper stem tip color gene, and the primers and the kit developed according to the KASP molecular marker can be used for identifying the pepper stem tip color and identifying the seed purity, can be detected at a seedling stage, and the identification method is carried out indoors, so that influences of natural factors such as weather, diseases and insect pests are avoided, and true and false hybrid seeds can be identified early, fast and accurately, and the seeds can be put on the market early.

The present application relates to the field of molecular genetic technology, in particular to a KASP molecular marker TMSK23 related to eggplant reverse temperature-sensitive male sterility and application thereof. The molecular marker site is obtained by crossing 05ms as female parent and S132 as male parent, and obtaining F2 separation population. According to the published high-quality eggplant genome information, the whole genome resequencing technology is used to pool and sequence the homozygous dominant fertile plants and homozygous recessive sterile plants in the F2 generation of eggplant, and the SNP difference sites of the fertile gene and sterile gene pool are screened out, and the difference sites are verified by KASP gene typing method and protein three-dimensional structure prediction. The present application solves the problems of large workload in hybrid seed production in the prior art, avoids the problem of a large number of measurement and matching in the transgenic process, improves the breeding efficiency and has other advantages.

The utility model discloses a kind of tomato hybrid seed production pollination devices, belong to pollination device technical field, including device ontology and conveying pipe, the side fixed mounting of device ontology has conveying pipe, the side of conveying pipe is provided with spray head, connector is fixedly installed on spray head, the both sides of connector are fixedly installed with convex post, the both sides of conveying pipe are provided with sliding slot;The tomato hybrid seed production pollination device is replaced by being installed with the connector of quick replacement to realize connector convenient replacement, when when replacing connector needs to be installed, let the convex post of connector two sides align the sliding slot on conveying pipe, the convex post is slid into the sliding slot, interface will be into connector, connector extrusion push plate, then rotate connector and rotate the convex post into notch, spring rebound, connector is fixed, connector is not only quickly disassembled by being installed with the connector of quick replacement, different connector can be replaced according to different varieties of tomato to meet the pollination demand of tomato, and also facilitate maintenance.

The utility model relates to the technical field of tomato planting pollination, in particular to a pollination device for tomato hybrid seed production. Comprising a round pipe with one end open, a flow guide cover is arranged at the open end of the round pipe, a fan is arranged in the round pipe, an air inlet hole penetrates through the end, away from the flow guide cover, of the round pipe, a handle is fixedly installed at the bottom of the round pipe, a switch assembly is arranged on the side, close to the flow guide cover, of the handle, and an installation block is fixedly installed at the top of the round pipe. The device comprises a mounting block, a first cavity is formed in the mounting block, a feeding groove communicated with the first cavity is formed in the top of the mounting block, a pollen bottle is arranged at the top of the mounting block, a bottle opening of the pollen bottle is slidably mounted in the feeding groove, and a rotating roller is rotatably mounted in the first cavity. Through a simple uniform discharging structure, pollen can be uniformly discharged conveniently, so that the pollen discharging amount of each pollination operation is approximately consistent, the problem that the pollen spraying amount is small or large is avoided, and the tomato pollination effect is guaranteed.