318 results about "Hybrid seed" patented technology

A Chinese cabbage hybrid seed production marking method relates to the technical field of Chinese cabbage hybrid seed production. The technical scheme of the method is as follows: after Chinese cabbage hybrid seed production pollination is finished, firstly holding the pedicel of a pollinated flower with the left hand; then transversely tearing off two sepals from the root with the right hand, wherein light movement is required. The Chinese cabbage hybrid seed production marking method can perform marking without rope tying, ring arranging or stamp-pad ink coating, and is low in cost and high in accuracy.

The invention discloses an acer elegans cross breeding method based on flowering phase difference regulation and control, and belongs to the technical field of garden plant breeding. The method comprises the following steps: selecting an acer elegans variety of which the natural early flowering phase difference is greater than or equal to 10 days and the genetic distance is greater than or equal to 0.5 as a cross parent, carrying out low-temperature refrigeration and shading blue light supplementing treatment on a female parent to delay the flowering phase, and carrying out prolonged illumination, red light supplementing and hormone and nutrient solution spraying on a male parent to advance the flowering phase; male parent pollen is collected through a vacuum negative pressure adsorption device and stored in a closed container, a sucrose solution is sprayed to female parent stigmas before pollination, and repeated pollination is conducted through a directional nozzle at 9-11 o'clock every day; carrying out stratification treatment and temperature-difference temperature-control germination on the hybrid seeds, and carrying out light-temperature regulation and control seedling culture after sowing; the molecular marker is used for verifying the hybridization authenticity and target traits, phenotype determination is carried out on three-year-old seedlings to complete breeding, the hybridization obstacle caused by asynchronous flowering phases of the caenorhabditis elegans varieties is solved through the flowering phase regulation and control technology, and the hybridization success rate is increased.

The invention provides a method for sterile sowing and efficient propagation of cymbidium faberi. The method comprises the following steps: pretreatment of cymbidium faberi seeds, sterile germination of the cymbidium faberi seeds, protocorm proliferation, differentiation, seedling strengthening and acclimatization and transplantation. The pretreatment comprises the steps of refrigerating hybrid seeds of cymbidium orchid at 4 DEG C, performing secondary disinfection with sodium hypochlorite, performing dark culture for 10-30 days after sowing, and then performing light culture, and the germination culture medium is prepared by taking 1 / 4 MS as a basic culture medium and adding 100-200ml / L of coconut water, 0.5-1.0 mg / L of 6-benayl aminopurine, 0.1-0.5 mg / L of naphthylacetic acid, 0.5-2.0 g / L of peptone and 0.5-1.0 g / L of activated carbon. According to the method, the seed germination rate can be effectively increased, the 60d seed germination rate reaches up to 85%, the pollution rate is reduced to 5% or below, 50.0 g / L inositol, 2.0 mg / L glycine and 2.0 mg / L nicotinic acid are added into a differentiation culture formula, the differentiation rate can be effectively increased, the leaf bud differentiation rate reaches up to 90%, meanwhile, rooting can be induced, the growth time of cymbidium faberi from seeds to seedlings is greatly shortened, a large number of high-quality seedlings can be obtained within a short time, and the method is suitable for large-scale popularization and application. Technical data are accumulated for cymbidium resource protection and new variety cultivation.

A method is provided for determining soil properties for an area of land, from soil spectrum data. In an embodiment, the method includes receiving soil spectrum data records from hyperspectral sensors that represent a mean soil spectrum of a specific geo-location of the area of land and removing interference signals from the spectrum data records to create soil spectral bands. The method also includes predicting a plurality of soil property datasets based on a partial least-square regression and the soil spectral bands and selecting specific soil property datasets from the plurality of soil property datasets to represent soil properties of the specific geo-location, wherein the specific soil property datasets include property data and spectral band data for spectral bands used to determine the property data. The specific soil property datasets may then be used to generate a crop prescription of recommended hybrid seeds or population densities for the specific geo-location.

The invention discloses a male sterility gene ZmUCE4 and application thereof in creating a male sterility line of corn, and belongs to the field of plant biotechnology breeding. According to the invention, site-directed mutagenesis of the ZmUCE4 gene in wild type corn is carried out through a CRISPR / Cas9 gene editing technology, pollen development abnormality and abortion can be caused, complete male sterility is caused, and it is proved that the ZmUCE4 gene has a regulation and control function on corn male reproductive development. Through offspring screening, a sterile line without transgenic components can be obtained, a stable maize male sterile line is created, and the method has important significance on maize male fertility control and hybrid seed production. The invention also designs a functional molecular marker aiming at the obtained uce4 male sterility mutant gene, and has important application value in maize male sterility line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of a GhDCP5L gene in creation of high-temperature response cotton germplasm, and an amino acid sequence of a protein coded by the GhDCP5L or a homologous gene thereof is as shown in SEQ ID NO.2 or SEQ ID NO.4. The invention provides a novel thermal response gene, a gene resource library of high-temperature response of cotton is enriched, the GhDCP5L gene and a homologous gene thereof are applied to plant genetic engineering breeding, and new germplasm of high-temperature-resistant or sensitive high-temperature-resistant cotton can be accurately created by regulating and controlling the expression of the gene, so that the high-temperature-resistant or sensitive high-temperature-resistant cotton is obtained. The method provides technical support for coping with stable yield of cotton under the background of global warming climate, can be used for preparing male sterile line cotton germplasm by performing high-temperature treatment on the high-temperature-sensitive material, has good application value in the aspects of cotton hybrid seed production, promotion of hybrid cotton development and the like, and is beneficial to improving the economic benefit of the cotton industry.

The invention discloses an InDel marker primer for identifying authenticity of sisal hemp interspecific hybridization F1 generation and application of the InDel marker primer. According to re-sequencing data of parent varieties of sisal hemp, an InDel marker primer for identifying the authenticity of a hybrid F1 generation of sisal hemp '292' and 'Induced 7' is developed, identification is carried out in a seedling stage of the hybrid F1 generation, and a result shows that offspring which can simultaneously amplify male parent and female parent characteristic bands is a true hybrid; the hybrid seeds are regarded as false hybrids when any one stripe is lacked or two stripes are lacked, so that the detection purity of the F1-generation real hybrid seeds is improved, and the identification time is advanced. Meanwhile, compared with a traditional hybrid identification method, the method for identifying the authenticity of the sisal hemp variety interspecific hybridization F1 hybrid is rapid, convenient, accurate, reliable, short in breeding period and low in breeding cost, breeding target offspring can be screened in advance, the identification efficiency of sisal hemp interspecific hybrids is effectively improved, and the method is suitable for popularization and application. And a more effective method is provided for rapidly identifying the authenticity of sisal hemp interspecific filial generations.

The invention discloses a production method of hybrid rice seeds, and belongs to the field of agricultural breeding. According to the method, high-purity hybrid seeds are produced through the technical means of excellent female parent reproductive line cultivation, reproductive line reproduction, preliminary fluorescent sorting, further fine seed screening, artificial seed shell adding, hybrid seed production and the like. According to the method, the technical problem that the sorting effect and the seed purity of colorless seeds and colored fluorescent seeds are influenced due to the fact that the precision of a color sorting technology cannot reach 100% due to difference or abnormal development of maturity of reproductive line seeds of a common nuclear male sterile line can be solved, and sterile line seeds meeting the market standard are obtained.

The invention discloses a gene ZmKO1 for regulating flowering and pollination of corn male flower spikelet and application of the gene ZmKO1 in corn male sterility hybrid seed production, and belongs to the field of plant genetic engineering. The invention provides a gene ZmKO1 for regulating and controlling flowering and pollination of maize male flower spikelet, and the phenomenon of nuclear male sterility can be caused by mutation of the gene ZmKO1. It is also clear that on the basis of the ZmKO1 gene locus, a CRISPR-Cas9 technology or a mutant gene backcross transformation technology is utilized, and cultivation of the maize nuclear male sterile line can be achieved. The invention also finds that in the spinning period of the sterile line, the male flower fertility of the sterile line can be recovered by spraying exogenous gibberellin to tassels, normal selfing propagation can be realized, and the sterile line can be economically and efficiently maintained as a maintainer line. Therefore, the cell nucleus male sterile line created based on the ZmKO1 gene locus has the characteristic of dual purposes and can be used for maize male sterility hybrid seed production, and the invention also provides a technical system for realizing maize two-line method sterile hybrid seed production by utilizing ZmKO1 gene mutation.

The invention relates to a gene ZmILR1 for controlling the size of a corn kernel as well as a molecular identification method and application of the gene ZmILR1, and belongs to the technical field of plant genetic breeding and functional gene research. The ZmILR1 gene encodes an IAA-amino acid hydrolase, mutation of the IAA-amino acid hydrolase causes corn kernels to become smaller, and overexpression of the gene can create a corn material with increased kernel length, kernel width and hundred-kernel weight. A functional molecular marker A1A4 is developed according to the sequence difference of the mutant gene and a wild type gene at the DNA level, and can be used for rapid identification and screening of small-grain corn materials. The invention provides a theoretical basis and an application tool for analyzing a molecular mechanism of the corn grain size and developing molecular marker-assisted breeding and hybrid seed breeding, and provides important gene resources and technical support for high-yield and high-quality molecular breeding of corn.

An example seed structure, systems, and methods for conducting crystal growth processes are provided. In one example, the present disclosure provides an example seed structure for a silicon carbide crystal growth system. The seed structure includes a carrier layer. The carrier layer is silicon carbide. The seed structure includes a seed layer bonded to the carrier layer with a bond. The seed layer is crystalline silicon carbide. The seed layer provides a growth surface for growing a crystalline silicon carbide structure in a silicon carbide crystal growth process.

The invention belongs to the field of molecular markers and detection thereof, and particularly relates to an SNP (Single Nucleotide Polymorphism) site primer combination for identifying the authenticity of tomato varieties and the purity of hybrids and application of the SNP site primer combination. The three SNP primer combinations provided by the invention can be respectively used for performing early identification on a common tomato variety, a cherry tomato variety and a taste tomato variety in a seed or seedling stage, so that the authenticity of the varieties and the purity of hybrids are ensured, and meanwhile, whether two or more unknown tomato varieties belong to the same variety or not can be identified; and the hybrid purity of the tomato variety to be detected. The rights and interests of producers and breeders can be practically protected, and technical support is provided for protection of tomato germplasm resources and new varieties.

The present invention relates to diploid, fertile, self-compatible and essentially homozygous potato lines, wherein said lines comprise an agronomically desirable trait such as vigour. The invention further relates to methods for producing such plants and to hybrid seeds obtained by crossing such homozygous potato lines and to potato plants grown from said seed.

The invention belongs to the technical field of crossbreeding, and particularly relates to a castration hybridization method of amphoteric asparagus. The method comprises the following steps: selecting an amphoteric asparagus in a bud pre-mature period as an asparagus maternal plant; petals and stamen groups of flower buds on the maternal plants are pinched off on the pollination day, and bare stigmas are left; picking mature and pollinated male flowers as male parent flowers on the pollination day; adhering the collected male parent pollen to the castrated female parent flower stigma after 4 p.m. at the temperature of 25-35 DEG C in clear weather from April to June every year, bagging and isolating to obtain homozygous seeds. The efficient method is provided for hybridization of the amphoteric asparagus for the first time, variety mixing in the asparagus hybrid seed production process is eradicated, the purity of the seeds is guaranteed, and the consistency of seeds for later production and the correctness of experimental research are guaranteed. In addition, the method can obtain the amphoteric asparagus with stable hereditary characteristics, and has guiding significance for the hybridization technology of the amphoteric asparagus.

The invention relates to a nucleic acid molecule for detecting a transgenic maize event DBN9641 containing a universal hybrid seed production (UHT) and a detection method of the nucleic acid molecule, and the nucleic acid molecule comprises SEQ ID NO: 1 or a complementary sequence of the SEQ ID NO: 1, and SEQ ID NO: 2 or a complementary sequence of the SEQ ID NO: 2. The corn plant DBN9641 is endowed with a function of keeping sterility of a sterile line in a hybrid seed production technology, so that a castration step in a hybrid seed production process is omitted, manpower and material resources are saved, the operation is simple, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic corn event DBN9641 or not.

The present invention relates to diploid, fertile, self-compatible and essentially homozygous potato lines, wherein said lines comprise an agronomically desirable trait such as vigour. The invention further relates to methods for producing such plants and to hybrid seeds obtained by crossing such homozygous potato lines and to potato plants grown from said see.

The utility model relates to the technical field of screening devices, in particular to a corn hybrid seed screening device. Comprising a screening box and a vibration assembly, the vibration assembly vibrates the screening box, the top of the screening box is hollowed out, a plurality of screening drawers and first collecting drawers are slidably arranged on the screening box, the screening drawers and the first collecting drawers are each provided with a limiting assembly, the limiting assemblies are connected with the screening box in a clamped mode, the screening box slides in an outer box, and the vibration assembly is arranged at the bottom in the outer box. A conveying cylinder is arranged at the top in the outer box, a spiral conveying assembly is arranged in the conveying cylinder, a feeding port penetrating through the conveying cylinder is formed in the top of the outer box, a discharging port penetrating through the interior of the outer box is formed in the bottom of the conveying cylinder, a dust suction pipe penetrating through the conveying cylinder is arranged at the top of the outer box, a containing frame is fixed to one side of the outer box, and a dust collecting assembly is arranged between the dust suction pipe and the containing frame. According to the corn hybrid seed screening device, part of dust can be collected in advance before screening, and the screening drawer and the collecting drawer are not prone to being vibrated out of the screening box.

The invention discloses a method for shortening a grape breeding fruiting period, which comprises the following steps: S1, sowing: accelerating germination and sowing hybrid seeds subjected to low-temperature stratification treatment in the first ten days of February to obtain seedlings; s2, transplanting: in the middle ten days of March, when the seedlings obtained in the S1 grow 5-6 true leaves, transplanting the seedlings into a ridged greenhouse nursery garden; s3, primary grafting: in the last ten days of April, selecting semi-lignified branches with the thickness of more than 0.4 cm and full bud eyes of hybrid seedlings, and grafting the semi-lignified branches to current-year semi-lignified new tips of the cutting seedlings of the rootstock of the Zhichang anti-rootstock No.1; s4, secondary grafting: in the last ten days of May, when the young sprouts of the hybrid seedlings after primary grafting grow to 30-50cm and the thickness is 0.4 cm or more, buds on the semi-lignified young sprouts are adopted for secondary grafting. The method is carried out in a greenhouse, current-year grafting and current-year forming can be achieved by adopting a high-position grafting flat shed erecting potential, the child process is manually intervened, the trunk growth time is skipped, and flowering and fruiting as soon as possible are promoted by utilizing the powerful root system of the Zhichang anti-anvil No.1.

The present application provides an isolation, cloning, functional verification of a restorer gene OsRf19 for cytoplasmic male sterility in rice and its use in improving rice. The present application specifically provides a nucleotide sequence of the gene OsRf19 and an encoded amino acid sequence thereof, an expression vector comprising the nucleotide sequence of the gene OsRf19, a method of creating a restorer line of rice using the gene OsRf19, and a method of restoring the fertility of a cytoplasmic male sterility line of rice and producing rice hybrid seeds, and related uses.

The invention relates to the technical field of plant genetic engineering and molecular genetics, and discloses a rice appearance quality gene identification and molecular marker-assisted breeding method, which comprises the following steps: constructing a knockout material of a rice grain appearance regulation gene FN20; matching the mutant fn20 with a rice strain FN20 with normal rice grain appearance; a material containing a rice grain appearance quality regulation gene FN20 is constructed, and the rice appearance quality gene identification and molecular marker-assisted breeding method comprises the following steps: cloning the rice grain appearance quality regulation gene FN20 (OsPFPB) from a rice grain appearance quality regulation mutant fn20 by utilizing a map-based cloning technology; the method comprises the following steps: knocking out an FN20 (OsPFPB) gene in rice by using a gene editing system to obtain a homozygous strain with changed rice grain appearance quality; the FN20 (OsPFPB) gene can be applied to the field of rice breeding, and has important practical significance on removal of false hybrids in the early stage of hybrid seed production, namely seed purity identification and molecular-assisted selection breeding application of oriented rice grain appearance quality improvement.

The invention belongs to the field of plant biotechnology breeding, and relates to an application of a ZmGalAT1 gene and an encoding protein thereof in corn fertility control. According to the invention, a corn male sterility mutant galat1 is obtained by EMS mutagenesis, and a gene ZmGalAT1 for controlling corn male flower development is identified through map-based cloning. The gene is subjected to site-directed mutagenesis in wild type corn through a CRISPR / Cas9 gene editing technology, three galat1 allelic corn male sterility mutants are created, corn male flowers of the mutants are completely aborted, sterile mutants without transgenic ingredients can be obtained through offspring screening, and the mutants are further applied to corn sterile breeding and seed production. The invention also designs functional molecular markers for three allelic male sterile mutants of GalAT1, and has important application value in maize male sterile line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The present invention relates to hybrid barley seed production and marker assisted selection of barley plants in order to obtain barley plants or seeds comprising an Ant 1 and / or Ant2 gene allowing color sorting of seeds.

The application discloses InDel marker primers for identifying authenticity or seed purity of a cauliflower variety Jinpin 81 and a method for identifying the same. The application screens and designs specific InDel sites and selects five pairs of InDel marker primers from the specific InDel sites by analyzing parent resequencing data of the cauliflower variety 'Jinpin 81' in a whole genome range, and the nucleotide sequences of the five pairs of InDel marker primers are respectively shown in SEQ ID NO. 1-SEQ ID NO. 10. The identification method established by applying the five pairs of marker primers can quickly and accurately complete identification of authenticity of the cauliflower variety 'Jinpin 81' and purity of hybrid seeds thereof, solves problems of long cycle, high cost and low accuracy existing in traditional identification methods, has advantages of high identification accuracy, simple and fast operation, small sample requirement and the like, greatly improves identification efficiency and accuracy, and effectively guarantees seed production quality of the cauliflower variety 'Jinpin 81'.

The invention provides a recombinant TKC vector applied to CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated 9) and a construction method of a mutant plant, and belongs to the technical field of gene editing. The invention provides a recombinant TKC vector applied to CRISPR-Cas9, the recombinant TKC vector comprises a U6 transcription box and / or a U3 transcription box, a target gene in the transcription box is located between a promoter and a terminator, and transcription expression of the target gene can be effectively guaranteed. By using the recombinant TKC vector and knocking out the EMF1 gene in a japonica rice dry-hui 89 material, the daily flowering time of the japonica rice dry-hui 89 material is obviously earlier, the flowering time of hybrid seed production of indica rice and japonica rice is encountered, the yield of hybrid seed production of indica rice and japonica rice is favorably improved, and the seed production cost is reduced. By utilizing the gene editing method provided by the invention, the daily flowering time of the japonica rice variety, i.e., the Yanhui 89 can be obviously advanced, and the problem of infertility during flowering during indica-japonica hybrid seed production is solved.

The invention belongs to the technical field of cassava planting, and discloses a method for improving the germination rate and transplanting survival rate of cassava hybrid seeds, and the method comprises the following steps: S1, soaking the cassava hybrid seeds in a potassium permanganate solution, and then soaking in a glucose solution; s2, weighing the garden soil, the fine sand and the fermented sugarcane filter mud in proportion; s3, sowing the pretreated seeds on a seedling culture substrate; s4, placing the seedling raising tray in an environment with the temperature of 25-30 DEG C, the humidity of 70%-80% and the illumination intensity of 3000-5000 lux for accelerating germination, and when the germination rate reaches 80%, moving the seedling raising tray out for transplanting; s5, the roots of the seedlings are soaked in a rooting powder solution, then the seedlings are transplanted into a field or a nutrition bowl prepared in advance, and a sunshade net is used for sunshade treatment 3 days before transplanting. The problems that existing cassava hybrid seeds are low in germination rate and low in seedling transplanting survival rate are solved.

The utility model discloses efficient hybrid seed cultivation equipment in a corn variety breeding process, and belongs to the technical field of corn hybrid seed cultivation. The efficient hybrid seed cultivation equipment in the corn variety breeding process comprises a cultivation box and further comprises a fixing frame, the fixing frame is located in the cultivation box, a plurality of cultivation boxes are arranged in the fixing frame, and a water tank is arranged at the upper end of the cultivation box. In order to solve the problems that according to an existing cultivation box, it is inconvenient for personnel to observe and evaluate corn seedlings, the corn seedlings are prone to mutual shielding, and consequently seedling character evaluation is difficult to conduct, cultivation boxes are installed in a fixing frame, the multiple cultivation boxes are distributed in a step shape, and the corn seedlings are located in the cultivation boxes; due to the fact that the height difference exists between the two longitudinal cultivation boxes and the two transverse cultivation boxes are separated, a larger growth space is provided for corn seedlings, meanwhile, personnel can observe and evaluate the seedlings conveniently, and the breeding efficiency is improved.

The invention provides a stigma viscosity adjusting solution and a method for improving the pollination rate of bananas, and belongs to the technical field of banana breeding. The stigma viscosity adjusting solution is prepared from the following components in percentage by mass: 0.008 to 0.012 percent of boric acid, 5 to 7 percent of cane sugar, 0.02 to 0.04 percent of calcium nitrate tetrahydrate, 0.008 to 0.012 percent of potassium nitrate and the balance of water. The stigma viscosity adjusting solution can provide a material basis required by banana pollen germination, promote pollen germination and elongation, increase stigma viscosity and facilitate adhesion of more pollen, and before banana pollination, the stigma viscosity adjusting solution is sprayed to female parent plant stigmas, so that more hybrid seeds can be obtained. The method can be widely applied to banana breeding.

The invention relates to the technical field of molecular identification of muskmelon hybrid seed purity, discloses an SSR primer and a method for rapidly identifying muskmelon hybrid seed purity, and aims to solve the problems that traditional seed purity detection depends on field test and manual observation, the detection period is long, the cost is high and the like. The invention provides two SSR primers with high polymorphism and good specificity for the melon hybrid 'Suqian No.4' and the specific markers thereof, and the SSR primers are applied to purity identification of 'Suqian No.4' seeds, so that good technical support is provided for seed production and seed sales, and the result is reliable. The method has the advantages of rapidness, accuracy, low cost, good repeatability, strong operability and the like.

The present disclosure provides methods for increasing yield in a hybrid seed production field and for allocating resources for hybrid seed production. In certain aspects, the methods for increasing yield in a hybrid seed production field utilize learned prediction models to determine optimal planting dates or management practices during plant growth and development.

The invention relates to a pepper promoter CanASP28 and application thereof, and belongs to the technical field of gene engineering. The nucleotide sequence of the pepper promoter CanASP28 is shown as SEQ ID NO.5, and the pepper promoter CanASP28 can drive specific expression of anther in plants, can be used for creating male sterile lines, crop hybrid seed production and crop crossbreeding, can improve the seed purity and hybrid seed production and crossbreeding efficiency, and can be applied to the field of plant breeding. Therefore, an effective tool gene is provided for anther specific expression regulation and pollen expression regulation of pepper and other solanaceae crops.