152 results about "Hybrid seed" patented technology

A Chinese cabbage hybrid seed production marking method relates to the technical field of Chinese cabbage hybrid seed production. The technical scheme of the method is as follows: after Chinese cabbage hybrid seed production pollination is finished, firstly holding the pedicel of a pollinated flower with the left hand; then transversely tearing off two sepals from the root with the right hand, wherein light movement is required. The Chinese cabbage hybrid seed production marking method can perform marking without rope tying, ring arranging or stamp-pad ink coating, and is low in cost and high in accuracy.

The invention discloses a male sterility gene ZmUCE4 and application thereof in creating a male sterility line of corn, and belongs to the field of plant biotechnology breeding. According to the invention, site-directed mutagenesis of the ZmUCE4 gene in wild type corn is carried out through a CRISPR / Cas9 gene editing technology, pollen development abnormality and abortion can be caused, complete male sterility is caused, and it is proved that the ZmUCE4 gene has a regulation and control function on corn male reproductive development. Through offspring screening, a sterile line without transgenic components can be obtained, a stable maize male sterile line is created, and the method has important significance on maize male fertility control and hybrid seed production. The invention also designs a functional molecular marker aiming at the obtained uce4 male sterility mutant gene, and has important application value in maize male sterility line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The invention relates to a gene ZmILR1 for controlling the size of a corn kernel as well as a molecular identification method and application of the gene ZmILR1, and belongs to the technical field of plant genetic breeding and functional gene research. The ZmILR1 gene encodes an IAA-amino acid hydrolase, mutation of the IAA-amino acid hydrolase causes corn kernels to become smaller, and overexpression of the gene can create a corn material with increased kernel length, kernel width and hundred-kernel weight. A functional molecular marker A1A4 is developed according to the sequence difference of the mutant gene and a wild type gene at the DNA level, and can be used for rapid identification and screening of small-grain corn materials. The invention provides a theoretical basis and an application tool for analyzing a molecular mechanism of the corn grain size and developing molecular marker-assisted breeding and hybrid seed breeding, and provides important gene resources and technical support for high-yield and high-quality molecular breeding of corn.

An example seed structure, systems, and methods for conducting crystal growth processes are provided. In one example, the present disclosure provides an example seed structure for a silicon carbide crystal growth system. The seed structure includes a carrier layer. The carrier layer is silicon carbide. The seed structure includes a seed layer bonded to the carrier layer with a bond. The seed layer is crystalline silicon carbide. The seed layer provides a growth surface for growing a crystalline silicon carbide structure in a silicon carbide crystal growth process.

The present invention relates to diploid, fertile, self-compatible and essentially homozygous potato lines, wherein said lines comprise an agronomically desirable trait such as vigour. The invention further relates to methods for producing such plants and to hybrid seeds obtained by crossing such homozygous potato lines and to potato plants grown from said seed.

The present application provides an isolation, cloning, functional verification of a restorer gene OsRf19 for cytoplasmic male sterility in rice and its use in improving rice. The present application specifically provides a nucleotide sequence of the gene OsRf19 and an encoded amino acid sequence thereof, an expression vector comprising the nucleotide sequence of the gene OsRf19, a method of creating a restorer line of rice using the gene OsRf19, and a method of restoring the fertility of a cytoplasmic male sterility line of rice and producing rice hybrid seeds, and related uses.

The invention belongs to the field of plant biotechnology breeding, and relates to an application of a ZmGalAT1 gene and an encoding protein thereof in corn fertility control. According to the invention, a corn male sterility mutant galat1 is obtained by EMS mutagenesis, and a gene ZmGalAT1 for controlling corn male flower development is identified through map-based cloning. The gene is subjected to site-directed mutagenesis in wild type corn through a CRISPR / Cas9 gene editing technology, three galat1 allelic corn male sterility mutants are created, corn male flowers of the mutants are completely aborted, sterile mutants without transgenic ingredients can be obtained through offspring screening, and the mutants are further applied to corn sterile breeding and seed production. The invention also designs functional molecular markers for three allelic male sterile mutants of GalAT1, and has important application value in maize male sterile line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The present invention relates to hybrid barley seed production and marker assisted selection of barley plants in order to obtain barley plants or seeds comprising an Ant 1 and / or Ant2 gene allowing color sorting of seeds.

The application discloses InDel marker primers for identifying authenticity or seed purity of a cauliflower variety Jinpin 81 and a method for identifying the same. The application screens and designs specific InDel sites and selects five pairs of InDel marker primers from the specific InDel sites by analyzing parent resequencing data of the cauliflower variety 'Jinpin 81' in a whole genome range, and the nucleotide sequences of the five pairs of InDel marker primers are respectively shown in SEQ ID NO. 1-SEQ ID NO. 10. The identification method established by applying the five pairs of marker primers can quickly and accurately complete identification of authenticity of the cauliflower variety 'Jinpin 81' and purity of hybrid seeds thereof, solves problems of long cycle, high cost and low accuracy existing in traditional identification methods, has advantages of high identification accuracy, simple and fast operation, small sample requirement and the like, greatly improves identification efficiency and accuracy, and effectively guarantees seed production quality of the cauliflower variety 'Jinpin 81'.

The invention provides a recombinant TKC vector applied to CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated 9) and a construction method of a mutant plant, and belongs to the technical field of gene editing. The invention provides a recombinant TKC vector applied to CRISPR-Cas9, the recombinant TKC vector comprises a U6 transcription box and / or a U3 transcription box, a target gene in the transcription box is located between a promoter and a terminator, and transcription expression of the target gene can be effectively guaranteed. By using the recombinant TKC vector and knocking out the EMF1 gene in a japonica rice dry-hui 89 material, the daily flowering time of the japonica rice dry-hui 89 material is obviously earlier, the flowering time of hybrid seed production of indica rice and japonica rice is encountered, the yield of hybrid seed production of indica rice and japonica rice is favorably improved, and the seed production cost is reduced. By utilizing the gene editing method provided by the invention, the daily flowering time of the japonica rice variety, i.e., the Yanhui 89 can be obviously advanced, and the problem of infertility during flowering during indica-japonica hybrid seed production is solved.

The utility model discloses efficient hybrid seed cultivation equipment in a corn variety breeding process, and belongs to the technical field of corn hybrid seed cultivation. The efficient hybrid seed cultivation equipment in the corn variety breeding process comprises a cultivation box and further comprises a fixing frame, the fixing frame is located in the cultivation box, a plurality of cultivation boxes are arranged in the fixing frame, and a water tank is arranged at the upper end of the cultivation box. In order to solve the problems that according to an existing cultivation box, it is inconvenient for personnel to observe and evaluate corn seedlings, the corn seedlings are prone to mutual shielding, and consequently seedling character evaluation is difficult to conduct, cultivation boxes are installed in a fixing frame, the multiple cultivation boxes are distributed in a step shape, and the corn seedlings are located in the cultivation boxes; due to the fact that the height difference exists between the two longitudinal cultivation boxes and the two transverse cultivation boxes are separated, a larger growth space is provided for corn seedlings, meanwhile, personnel can observe and evaluate the seedlings conveniently, and the breeding efficiency is improved.

The present disclosure provides methods for increasing yield in a hybrid seed production field and for allocating resources for hybrid seed production. In certain aspects, the methods for increasing yield in a hybrid seed production field utilize learned prediction models to determine optimal planting dates or management practices during plant growth and development.

The invention relates to a pepper promoter CanASP28 and application thereof, and belongs to the technical field of gene engineering. The nucleotide sequence of the pepper promoter CanASP28 is shown as SEQ ID NO.5, and the pepper promoter CanASP28 can drive specific expression of anther in plants, can be used for creating male sterile lines, crop hybrid seed production and crop crossbreeding, can improve the seed purity and hybrid seed production and crossbreeding efficiency, and can be applied to the field of plant breeding. Therefore, an effective tool gene is provided for anther specific expression regulation and pollen expression regulation of pepper and other solanaceae crops.

This invention relates to the field of plant variety breeding technology, and in particular to a method for improving the hybrid seed setting rate of rice under high temperature conditions. The technical solution is as follows: A method for improving the hybrid seed setting rate of rice under high temperature conditions includes an operational procedure for improving the hybrid seed setting rate of rice under high temperature conditions. This invention transfers the pollination process indoors and uses an air conditioning system to maintain a stable temperature within a suitable range. Simultaneously, it utilizes hydroponics to provide a continuous and stable root moisture and local humidity environment for the pollinated plants, thereby creating a controlled microclimate. This not only avoids pollen inactivation and stigma drying caused by high temperature and dryness, but also prevents fertilization obstacles caused by drastic humidity changes, allowing pollen and stigma to successfully complete the pollination and fertilization process in a continuously suitable environment, thus effectively improving the hybrid seed setting rate.

The application discloses a preparation method of a hybrid seed layer assisted growth perovskite film and a perovskite-crystalline silicon laminated solar cell, and belongs to the technical field of solar cells. The perovskite film preparation method provided by the application comprises the following steps: (1) wetly depositing a hybrid seed layer solution on the surface of a substrate, and cooling after heat treatment to obtain a hybrid seed layer; and (2) dryly depositing an inorganic metal salt on the surface of the hybrid seed layer to form an inorganic metal salt layer, and then performing annealing treatment to obtain a perovskite film. The hybrid seed layer has the functions of crystal nucleus induction activity and interface regulation, a stable solid-solid reaction interface is constructed between the substrate and the precursor layer through a low-temperature liquid phase pre-deposition process, and bidirectional interface passivation, crystallization orientation regulation and energy level optimization are simultaneously realized, so that the photoelectric conversion efficiency and long-term operation stability of the laminated cell are significantly improved, and excellent batch preparation repeatability is achieved.

The invention discloses a smut-resistant sugarcane hybrid variety breeding method. The smut-resistant sugarcane hybrid variety breeding method comprises the specific steps that sugarcane hybrid seeds are cultivated into seedlings; inoculating the seedlings with smut fungus spores for disease resistance primary screening; then the plants are further screened through a double-track smut resistance test, and then screening of the high-quality sugarcane is achieved. According to the breeding method, the efficiency of breeding the high-quality smut-resistant sugarcane variety is improved, test errors caused by spore activity differences can be reduced, the test stability is improved, the smut resistance of ratoon sugarcane and newly-planted sugarcane is respectively tested by adopting a double-track smut-resistant test, and the breeding efficiency of the high-quality smut-resistant sugarcane variety is improved. Meanwhile, the influence of inoculation of smut fungi by adopting a spraying method and a dipping method on sugarcane screening can be observed, and the efficiency and comprehensiveness of breeding the smut-resistant sugarcane are improved.

This invention relates to the field of shape memory material preparation technology, specifically to a bio-based shape memory Eucommia ulmoides gum composite material and its preparation method. All components of the composite material are of entirely bio-based origin, using natural Eucommia ulmoides gum as the matrix. Nano-hybrid seed crystals composed of cellulose nanocrystals and carbon quantum dots are introduced. The cellulose nanocrystals act as nucleating agents, guiding and accelerating the cooling crystallization of the Eucommia ulmoides gum, while the carbon quantum dots form the shell, improving heat transfer efficiency. In the preparation process, this application uses triacylglycerol lipase as a biocatalyst, which can initiate an in-situ esterification reaction between the filler and the matrix at a low temperature of 65-75℃. This not only reduces energy consumption but also avoids the thermal degradation and damage to the Eucommia ulmoides gum macromolecular chains caused by high-temperature strong shear, achieving high performance and batch-to-batch stability of the material.

The application discloses a KASP molecular marker closely linked with pepper stem tip color, takes a zhangshugang version gene as a reference gene, and the KASP molecular marker is at 148515563 of a pepper 10th chromosome, a single nucleotide polymorphism at the position, and a base C to A replacement occurs at the position. The application further discloses primers, a kit for identifying the KASP molecular marker closely linked with the pepper stem tip color, and application of the KASP molecular marker in identifying the pepper stem tip color or identifying purity of pepper seeds. The KASP molecular marker closely linked with the pepper stem tip color gene, and the primers and the kit developed according to the KASP molecular marker can be used for identifying the pepper stem tip color and identifying the seed purity, can be detected at a seedling stage, and the identification method is carried out indoors, so that influences of natural factors such as weather, diseases and insect pests are avoided, and true and false hybrid seeds can be identified early, fast and accurately, and the seeds can be put on the market early.

The present application relates to the field of molecular genetic technology, in particular to a KASP molecular marker TMSK23 related to eggplant reverse temperature-sensitive male sterility and application thereof. The molecular marker site is obtained by crossing 05ms as female parent and S132 as male parent, and obtaining F2 separation population. According to the published high-quality eggplant genome information, the whole genome resequencing technology is used to pool and sequence the homozygous dominant fertile plants and homozygous recessive sterile plants in the F2 generation of eggplant, and the SNP difference sites of the fertile gene and sterile gene pool are screened out, and the difference sites are verified by KASP gene typing method and protein three-dimensional structure prediction. The present application solves the problems of large workload in hybrid seed production in the prior art, avoids the problem of a large number of measurement and matching in the transgenic process, improves the breeding efficiency and has other advantages.

The utility model discloses a kind of tomato hybrid seed production pollination devices, belong to pollination device technical field, including device ontology and conveying pipe, the side fixed mounting of device ontology has conveying pipe, the side of conveying pipe is provided with spray head, connector is fixedly installed on spray head, the both sides of connector are fixedly installed with convex post, the both sides of conveying pipe are provided with sliding slot;The tomato hybrid seed production pollination device is replaced by being installed with the connector of quick replacement to realize connector convenient replacement, when when replacing connector needs to be installed, let the convex post of connector two sides align the sliding slot on conveying pipe, the convex post is slid into the sliding slot, interface will be into connector, connector extrusion push plate, then rotate connector and rotate the convex post into notch, spring rebound, connector is fixed, connector is not only quickly disassembled by being installed with the connector of quick replacement, different connector can be replaced according to different varieties of tomato to meet the pollination demand of tomato, and also facilitate maintenance.

The invention discloses an efficient water lily hybridization method which comprises the following steps: selecting whether male parents need to be castrated or not according to whether plant female parents have selfing affinity or not, and castrating at dusk one day before water lily blooms or castrating in the morning next day so as to ensure that stigma fluid is still generated in the female parents after castrating; in addition, multiple kinds of water lily pollen are mixed together and then pollinated on the same female parent water lily, and then hybrid seeds are collected and sown. The method can improve the success rate of water lily hybridization and greatly shorten the time of water lily hybridization breeding; and the method is simple to operate and convenient to use, can be widely applied to water lily crossbreeding, and can provide help for innovation of water lily germplasm resources.

The utility model relates to the technical field of seed sowing, and discloses a corn hybrid seed sowing and soil covering device which comprises a mounting plate, a material collecting box is fixedly connected to the upper surface of the mounting plate, a discharging pipe is connected to the lower surface of the material collecting box in a penetrating mode, a motor is fixedly installed on one side of the discharging pipe, and the output end of the motor is fixedly connected with a sowing disc. A mounting frame is fixedly connected to the lower surface of the mounting plate, two connecting shafts are fixedly connected to one side of the mounting frame, connecting blocks are rotationally connected to the exteriors of the connecting shafts, a soil covering plate is fixedly connected to one side of each connecting block, and an adjusting assembly is arranged on one side of the mounting frame. According to the soil covering device, the soil covering angle of the soil covering plate can be conveniently adjusted, the angle of the soil covering plate can be conveniently adjusted according to the soil texture when seeds are sown, the seeds can be uniformly and appropriately covered with soil, and the soil covering angle can be conveniently increased according to the characteristics that sandy soil is large in soil particle and prone to loosening; and the thickness and the stability of soil covering can be increased.

The application discloses a male sterility gene Zmuce4 and application thereof in creating a maize male sterile line, and belongs to the field of plant biotechnological breeding. The application proves that the gene has a regulating function on maize male reproductive development by causing abnormal pollen development and abortion and leading to complete male sterility through site-directed mutation of the gene in wild-type maize by CRISPR / Cas9 gene editing technology. Zmuce4 Through offspring screening, a sterile line without a transgenic component can be obtained, and a stable maize male sterile line is created, which has important significance for maize male fertility control and hybrid seed production. uce4 The application also designs a functional molecular marker for the obtained male sterility mutant gene, which has important application value in maize male sterile line breeding, sterile hybrid seed production and molecular marker assisted selection.

The invention discloses a method for identifying the purity of a celery Jinrui 48 hybrid by using an InDel molecular marker. An InDel primer in the invention can generate a male parent specific marker and a female parent specific marker, and has strong specificity; according to the method disclosed by the invention, the celery 'Jinrui 48' hybrid seeds can be distinguished from other hybrids and male and female parents, and the purity of the hybrid seeds can be rapidly detected; the detection method disclosed by the invention has the advantages of rapidness, accuracy, low cost, convenience in operation and the like, can replace a traditional hybrid seed purity identification method, and has a relatively high commercial application value.

The application discloses a molecular marker co-segregated with a watermelon short-hair male sterility gene and application thereof, and belongs to the technical field of genetic engineering. The application can be directly used for molecular marker assisted breeding of the watermelon short-hair male sterility material, improves the selection efficiency of breeding, and speeds up the breeding process. It is worth noting that the short-hair male sterility material is different from the conventional male sterility material. The short-hair trait and the male sterility trait of the short-hair male sterility material are co-segregated, so in actual production, the male sterile plants can be screened from the male sterile maintainer line through the short-hair trait of the plants in the seedling stage, thereby obtaining 100% sterile male sterile plants, and the breeding time is saved. Meanwhile, the application lays a foundation for finally realizing cloning of the watermelon short-hair male sterility gene Clstms and regulation of the anther development and the epidermal hair development of the watermelon, thereby improving the hybrid seed production efficiency, shortening the breeding period, improving the seed purity, and reducing the seed production cost.

The invention provides a wheat hybrid seed production method which comprises the following steps: S1, identifying male and female parent ear development stages: in the later stage of wheat jointing, scanning field male and female parent plants by adopting machine vision and spectral analysis, and respectively identifying a target development stage of a male parent and a target development stage of a female parent; s2, differential spraying of a biological agent: spraying a humic acid compound preparation on the ear part of the male parent in an emphasized manner; s3, dynamically identifying a female parent in an emasculation period; s4, synchronous minimally invasive castration; according to the method, the development stages of male and female parents are accurately identified through machine vision and spectrums, and a humic acid compound preparation is sprayed in a differentiated manner, so that the synchronization window time of the flowering phase is prolonged, and pollination failure caused by extreme weather is avoided; target spikes are screened through a spike multi-feature recognition system, low-temperature minimally invasive castration is combined, the castration accuracy is improved, pistils are prevented from being scratched by manual castration, spike stalks are prevented from being damaged by mechanical castration, misjudgment of invalid spikes is eliminated, and the purity loss caused by extensive castration in the prior art is avoided.

The application belongs to the technical field of agricultural hybrid breeding, and discloses a pumpkin hybrid seed production method using rubber bands to bind and isolate pollination. The application aims to solve the problems of complicated isolation operation, easy flower damage, low efficiency and environmental pollution in the existing pumpkin seed production. The method comprises the following steps: in the afternoon of the day before pollination, a ring-shaped closed loop structure elastic rubber band is stretched and sleeved on the upper part of the female flower corolla of the female pumpkin, the elastic contraction force of the rubber band is used to close the front end of the corolla, and pre-binding isolation is realized; on the day of pollination, the rubber band on the female flower is removed to expose the stigma, and artificial pollination is performed; after the pollination is completed, the same rubber band is sleeved back to the original position immediately to tighten and isolate. Through the standardized process of pre-binding-unbinding-pollination-rebinding, the application has the advantages of simple operation, no flower damage, good isolation effect, single flower operation time of not more than 20 seconds, corolla damage rate of less than 2%, hybridization rate of 99%, low cost of rubber band, less pollution, and significant improvement of pumpkin hybrid seed production efficiency and seed purity.

The application provides an in-vitro seed embryo culture method of peony hybrid seeds, which comprises the following steps: 1) taking Chuan-shao No.1 or Chuan-shao No.2 as the female parent, Bozhou peony as the male parent to cross, collecting hybrid seeds, cleaning and disinfecting, taking embryos to obtain seed embryos; 2) taking the seed embryos, connecting into seed embryo starting seedling culture medium to culture, after the F1 generation seed embryo sprouts, first low-temperature treatment and then constant-temperature culture to obtain tissue culture seedlings; 3) taking the tissue culture seedlings to harden and transplant, which can be used. Under the culture of the specific culture medium, the double dormancy problem of the peony hybrid seeds is solved, and the low germination rate, high rot rate and embryo abortion of the peony hybrid seeds are overcome, so that the difficulty of the hybrid peony seedling quantity and quality is solved.

The utility model relates to the technical field of seed production, and discloses a corn hybrid seed production system, which comprises a shell, a feed inlet and a water sieve assembly, the water sieve assembly is arranged in the shell, the feed inlet is fixedly connected to the top of the shell, an air suction grid is fixedly connected to the bottom of the feed inlet, a material separation plate is fixedly connected to the interior of the air suction grid, and the water sieve assembly is arranged in the shell. The outer portion of the air suction grid is fixedly connected with an air suction opening, the front end of the shell is fixedly connected with a dust suction device, an air conveying pipe is fixedly connected between the dust suction device and the air suction opening, the air conveying pipe is fixedly connected into the shell, and the front end of the shell is fixedly connected with a dust storage box. According to the corn seed screening device, the seeds are divided through the material partition plate, the dust suction device is started to store impurities into the dust storage box, dust and impurities in the corn seeds are effectively removed, the situation that the corn seeds are mixed with the dust and the impurities and enter the screening box is prevented, the screening effect on the corn seeds is improved, and the practicability of the device is improved.