101 results about "Paphiopedilum" patented technology

The invention relates to a method for cross breeding and seedling propagation of paphiopedilum, which comprises the steps of artificial pollination, sterile seeding, strong seedling culture, and test-tube plantlet transplant. After the artificial pollination of paphiopedilum coenospecies, a powdery embryo in hybrid fruits is inoculated to a specially prepared seed germination culture medium for culture; plantlets obtained by the sterile seeding are inoculated to a specially prepared strong seedling culture medium for strong seedling culture; and finally, the test-tube plantlet transplant is performed. The method uses unique culture media, ensures the high germination rate, quick seedling and high seedling quality of paphiopedilum hybrid seeds, has the advantages of high operability, high application value and the like, and overcomes the difficulty that the paphiopedilum hybrid seeds are extremely difficult to germinate in a natural state due to the incomplete development of embryo and other difficulties.

The present invention relates to the abacterial seeding and tissue culture of Paphiopedilum. The Dendrobium is one kind of national first order protected plant and is hard to germinate naturally owing to its incomplete development of embryo. The present invention provides technology of abacterial seeding and tissue culture of Paphiopedilum to obtain great amount of test tube seedling via utilizing artificial nutritious liquid and abacterial seeding, proliferate small seedlings and obtain complete plants via rooting culture. The said seedling growing technology has the advantages of high germination rate, fast seedling growing rate, high seedling quality, etc.

A method for regulating and controlling the blooming period of paphiopedilum relates to a method for cultivating and managing paphiopedilum. The method comprises the following steps of changing water and fertilizer condition during cultivation process, promoting maturity of paphiopedilum plants, and applying phosphorus fertilizer or high-potash fertilizer; applying plant growth substance so as to improve resistivity and increase substance accumulation and promote maturity, dormancy, flower bud differentiation, germination and growth respectively; the ratio of N, P and K of the fertilizer is (8 to 12):(25 to 35):(15 to 25), the watering is performed at intervals of 10 days, and the water is manly used for sprinkling leaves. The method has the benefits that after six mouths, the blooming rate exceeds 66%, the problems that the paphiopedilum blooms irregularly, the blooming period is non-uniform, and the blooming rate is low are solved, one of the main problems that restrict the large-scale production of paphiopedilum is overcome, and the large-scale production of ornamental paphiopedilum in the country is promoted effectively. The economic benefit is highlighted, the consistency of blooming periods of batched plants is improved, the requirement of sale busy seasons can be met, the commodity rate of paphiopedilum is improved, and the economic benefit on production of paphiopedilum is improved therewith.

The invention discloses water-mist cultivating method for paphiopedilum, which cultivates paphiopedilum by combining selection of substrate with good air permeability with the water-mist cultivating method. The invention also discloses a device for realizing the water-mist cultivating method. A pot shelf used for placing the paphiopedilum pot is arranged on a cultivating shelf of which the bottom and four sides are sealed and is provided with a spraying and air-supplying facility to regularly ventilate and spray. The method disclosed in the invention can rapidly promote sprout of the paphiopedilum root, reduce dead root and rotten root, simultaneously promote aerial part to grow rapidly and healthily, and dramatically improve survival rate and quality of adult plant. In addition, the invention has low cost of the device and simple operation.

The invention discloses a method for tissue culture and rapid propagation of high-quality paphiopedilum maudiae seedlings. According to the method, after a series of pharmaceutical treatment is conducted on paphiopedilum maudiae stock plants, adventitious bud induction, clump shoot regeneration and rooting culture are conducted with newly grown lateral buds as the explants by means of a unique culture medium, and then rapid propagation of high-quality seedlings is achieved. An effective means is provided to meet the market requirement for high-quality paphiopedilum maudiae seedlings. The technique is practicable, and application value is high.

The invention belongs to the field of cultivation of plants, and specifically relates to a method for shortening a development period of Paphiopedilum Pfitzer fruits and improving germination rate of Paphiopedilum Pfitzer seeds. The method for shortening the development period of the Paphiopedilum Pfitzer fruits and improving germination rate of the Paphiopedilum Pfitzer seeds comprises the following steps: (1) when a pollinated flower of a Paphiopedilum Pfitzer plant falls down, sealing off a wound formed by disengagement of the flower with quicklime; and (2) when an ovary of the Paphiopedilum Pfitzer plant treated in the step (1) starts to swell, treating the developing ovary of the Paphiopedilum Pfitzer plant with a 6-BA solution for 12 to 36 h. According to the invention, through protection to the Paphiopedilum Pfitzer fruits and shortening of the development period of the ovary by treatment with the 6-BA solution, the influence of high temperature in summer on the development of the Paphiopedilum Pfitzer fruits is reduced, so embryo rate of the Paphiopedilum Pfitzer seeds and harvesting rate of the Paphiopedilum Pfitzer fruits are substantially improved.

The invention discloses a paphiopedilum maudiae potted flower production method and belongs to the technical field of plant cultivation. The paphiopedilum maudiae potted flower production method is characterized by comprising the following steps that firstly, infrastructure construction is performed; secondly, seedling exercising is performed; thirdly, transplantation is performed; fourthly, maintenance management in a small seedling stage is performed; fifthly, maintenance management in a middle seedling stage and in a large seedling stage is performed; sixthly, management in a mature flower forcing stage is performed; seventhly, management in a flower season is performed. According to the paphiopedilum maudiae potted flower production method, tissue culture seedlings of the paphiopedilum maudiae receive technology regulation such as seedling exercising, transplantation, seedling stage management, flower stage management and insect control, and therefore large-scale and industrialized production of paphiopedilum maudiae potted flowers is achieved. The paphiopedilum maudiae potted flower production method has the advantages of being simple in operation, short in period and the like.

PCT No. PCT/JP96/02998 Sec. 371 Date Jun. 10, 1997 Sec. 102(e) Date Jun. 10, 1997 PCT Filed Oct. 16, 1996 PCT Pub. No. WO97/14295 PCT Pub. Date Apr. 24, 1997The present invention provides a method for clone-multiplication of a Paphiopedilum wherein a scape terminal bud or an axillary shoot, whose surface has been sterilized, or an aseptic shoot obtained by an aseptic cultivation, of a Paphiopedilum stock, are cut into cross-sectional pieces which are then cultivated in a medium containing a plant hormone. By cutting an aseptic shoot containing a number of axillary buds into cross-sectional pieces and cultivating, a multishoot formation is feasible. Also by employing a liquid medium containing a solid support such as a rockwool or a paper filter in the culture, the plant death due to browning of tissues can effectively be prevented. In addition, by optimizing the method for sterilizing the buds before blooming, the medium composition and the culture condition, a large scale production of Paphiopedilums by means of a clone multiplication technology becomes feasible.

The invention discloses a paphiopedilum new variety 'yo-yo paphiopedilum' group rapid reproduction method, and relates to a biological engineering plant tissue culture reproduction method. The method includes the following steps of: 1, artificial cross pollination; 2, sterile sowing and protocorm formation stage; 3, proliferation and differentiation culture stage; 4, seedling strengthening and rooting stage; 5, seedling hardening and transplanting. The 'yo-yo paphiopedilum' combines the excellent traits of parents, has the advantages of being compact in plant type, unique and elegant in flower shape, attractive in flower color, easy to cultivate, up to 50 days in average flowering stage and the like, and avoids the disadvantages of being single in flower amount and too long in scape which is prone to be in lodging and broken. According to the method, the seed germination rate and the flowering rate are high, and the cultivation period is 3-4 years. The method is a high efficiency test tube reproduction method for obtaining a large number of 'yo-yo paphiopedilum' seedlings, and has the advantages of being high in germination rate, fast in seedling formation, good in seedling quality, strong in growth potential and the like, can obtain consistent test tube seedlings, and provides a large number of the seedlings in a short time so as to better protect wild resources.

The invention provides a Paphiopedilum GLO/PIMADS-box gene sequence and a polypeptide encoded by the gene sequence. In addition, the present invention also discloses a method for obtaining the clone of P. paphiopedilum GLO/PI gene. The method is to design a degenerate primer for the amino acid conservation region of the closely related species GLO/PIMADS-box gene, extract total RNA from the flower of P. -PCR amplification, combined with RACE technology to clone to obtain the full-length cDNA sequence of the GLO/PIMADS-box gene of Paphiopediphyllum. The P. paphiopedilum GLO/PIMADS-box gene of the present invention is a new gene related to the development of P. paphiopedilum. The P. paphiopedilum GLO/PI gene provided by the present invention not only lays a foundation for further research on the function of the P. paphiopedilum GLO/PI protein, but also provides a certain basis for the molecular mechanism of the paphiopedilum organ development.

The invention discloses paphiopedilum body wash. The paphiopedilum body wash is prepared from raw materials in parts by weight as follows: 10-20 parts of paphiopedilum, 5-8 parts of aloe, 5-8 parts of mint leaves, 4-6 parts of chaenomeles sinensis, 4-6 parts of fresh ginger, 3-5 parts of sulfur soap, 5-15 parts of almond oil, 1-3 parts of propylene glycol, 1-3 parts of sodium glyceryl monolaurate sulfate, 1-2 parts of citric acid, 1-3 parts of sodium chloride, 0.01-0.1 parts of sorbic acid, 0.05-0.1 parts of gardenia yellow pigment and 50-80 parts of deionized water. The invention further discloses a processing method of paphiopedilum body wash. The processing method comprises seven processing steps as follows: extraction, abstraction, boiling, blending, sampling detection, defoaming and inspection and can filling. The paphiopedilum body wash has slight faint scent, has functions of refreshing and restoring consciousness, moisturizing and beautifying skin, nourishing skin and retaining water, and is particularly suitable for middle-aged women and worthy of popularization.

The invention relates to a method for breeding and cultivating Paphiopedilum macranthum. More specifically, the invention provides a method for improving the germination rate of Paphiopedilum macranthum seeds. The method comprises the following steps: step 1, artificial pollination is carried out after the Paphiopedilum macranthum blooms; and (2) the seeds are obtained from 180-240 days after pollination and are sown on a seed germination culture medium, so that buds are obtained from the seeds. The invention further provides the method for cultivating Paphiopedilum macranthum, the seed germination rate is improved by adopting the method for improving the germination rate. The method can be used for producing the Paphiopedilum macranthum with the flower diameter of 12 cm, the inflorescence is complete, the petals are intact, the ornamental effect is good, the sowing germination rate is high, the growth speed of tissue culture seedlings is high, the survival rate reaches 95% or above, and the cultivation period can be shortened to three years.

The invention discloses a wide-spectrum sterile sowing culture medium II for tropical orchids. Each L of culture medium contains the following components by weight: 120g of NH4O3, 34g of KNO3, 440g of CaCl2.2H2O, 370g of MgSO4.7H2O, 90g of KH2PO4, 20g of saccharose, 2g of activated carbon, 5.8g of agar and the balance of 1/4MS microelements, organic components and iron salt; and the pH value of the culture medium is 5.7. The tropical orchids are cultured by the following steps: carrying culturing in a mature fruit pod period; and picking un-cracked fruit pods when fruit stems of the fruit pods starting to yellow, wherein the culture temperature is 24-26 DEG C, the illumination intensity is 60 micro mol.m/2s<-1>, and the illumination time is 12 hour/day. By utilizing the culture medium, the germination rate of paphiopedilum seeds is above 70%, the germination rates of cattleya, butterfly orchid and dendrobium seeds are above 90%, and the germination rates of other tropical orchid seeds are above 80%.

The invention belongs to the technical field of flower planting, which a particularly relates a cultivating method of paphiopedilum emersonii seedlings. The method comprises the following steps: making a culture room; performing seedling treatment; planting seedlings; fertilizing and transplanting. The cultivating method of paphiopedilum emersonii seedlings utilizes the self-made culture soil andthe culture room to cultivate the seedlings, providing an adaptive growth environment for the seedlings of the paphiopedilum emersonii. Also, in the treatment of seedlings, a mixed solution prepared from charcoal powder and water is used for soaking the root of the paphiopedilum emersonii seedlings, thereby solving problem that the mold can easily corrodes the seedlings of paphiopedilum emersoniiand cause the seedlings of the paphiopedilum emersonii to die. Therefore, the survival rate of paphiopedilum emersonii seedlings is improved. When planting, the moss powder is added into the self-madeculture soil, so that the porosity in the soil is improved, the growth of paphiopedilum emersonii is facilitated, and the goal of improving the survival rate of the paphiopedilum emersonii seedlingsis achieved.

The invention provides a method for breeding wenshan paphiopedilum seedlings by using somatic embryo. After mature wenshan paphiopedilum fruits are sterilized, the fruits are cut apart to obtain seeds, the seeds are inoculated in an induced germination medium to obtain young embryo, and the young embryo is inoculated in a liquid nutrient medium to obtain the somatic embryo; differentiation seedlings are obtained through differentiation cultivation; the differentiation seedlings are inoculated in a root medium to perform root cultivation, and finally the seedlings are exercised and transplanted, namely the wenshan paphiopedilum seedlings are obtained. The method for breeding wenshan paphiopedilum seedlings by using the somatic embryo obtains the young embryo after sterile germination of the wenshan paphiopedilum mature fruits, breaks dormancy of shoots, promotes germination of the seeds and growth of callus, performs rapid cultivation on the plant somatic embryo, enables wenshan paphiopedilum tissue cultivation enhancement factor to be improved to above 5 times from original 1 time, and greatly improves reproduction efficiency of the wenshan paphiopedilum. Survival rate of the wenshan paphiopedilum seedlings after transplanting reaches above 90%.

The invention discloses a cultivation substrate for paphiopedilum. The cultivation substrate comprises the following raw materials in parts by weight: 9-13 parts of vermiculite, 2-6 parts of dry pinebark, 3-6 parts of a boron fertilizer, 8-15 parts of a NPK mixed fertilizer, 25-35 parts of humus soil, 8-15 parts of dry pine sawdust, 5-7 parts of calcium carbonate powder, 3-7 parts of sucrose, and1-3 parts of aluminum sulfate. The invention also discloses a preparation method of the cultivation substrate, and a paphiopedilum division propagation method and seeding propagation method adoptingthe substrate, and the two propagation methods complement the cultivation substrate, and effectively improve a cultivation survival rate and average growth vigour of the paphiopedilum; the cultivationcycle of division propagation of the paphiopedilum is shortened by 13%, and the survival rate is improved by 8%; and the cultivation cycle of seeding propagation of the paphiopedilum is shortened by11%, and the survival rate is improved by 6.9%.

The invention provides a group of primers for separating paphiopedilum type B MADS-box genes comprising PahePI, PaheAP3Al, PaheAP3A2, PaheAP3B1 and PaheAP3B2 and a kit comprising the primers. The invention also provides a method for developing the primers. In addition, the invention provides a method of separating the type B MADS-box genes from a paphiopedilum plant through a nested PCR method by means of the primers.

The invention discloses an aseptic seeding medium for broad-spectrum tropical orchids; each L of the medium comprises 2 g of Hyponex No. 1, 1 g of tryptone, 20 g of banana, 100 g of mashed potato, 10 g of sucrose, 2 g of activated carbon, and 5.8 pg of agar, under pH of 5.7. Culturing is performed in the stage of mature capsules; to be specific, non-cracked capsules are picked from the time when stems of the capsules start to yellow. Culturing temperature is 24-26 DEG C, light intensity is 60 Mumol.m<-2>s<-1>, and illumination time is 12 h/d. After the use of the medium, germination rate of Paphiopedilum seeds is 70% and above, germination rate of Cattleya, Phalaenopsis and Dendrobium seeds is 90% and above, and germination rate of seeds of other tropical orchid is 80% and above.

The invention discloses a medium for aseptic sowing and rapid seedling of single-flower Paphiopedilum. The medium comprises a sowing medium, a subculture medium and a seedling-strengthening medium, wherein the sowing medium is composed of 1/4MS, tryptone with a concentration of 2 g/L, cane sugar with a concentration of 20 g/L and agar with a concentration of 5.8 g/L and has a pH value of 6; the subculture medium is composed of Huabao No. 1 with a concentration of 2 g/L, banana with a concentration of 10 g/L, smashed potato with a concentration of 100 g/L, cane sugar with a concentration of 20 g/L, active carbon with a concentration of 1 g/L and agar with a concentration of 5.8 g/L and has a pH value of 6; and the seedling-strengthening medium is composed of Huabao No. 1 with a concentration of 2 g/L, NAA with a concentration of 0.1 mg/L, 6-BA with a concentration of 0.2 mg/L, banana with a concentration of 10 g/L, smashed potato with a concentration of 100 g/L, cane sugar with a concentration of 20 g/L, active carbon with a concentration of 2 g/L and agar with a concentration of 5.8 g/L and has a pH value of 6. A harvesting period for mature capsules is that the capsules do not crack and the stem parts of the capsules begin to become yellow. The medium provided by the invention allows time for aseptic sowing and rapid seedling of Paphiopedilum to be shortened to 8 months from 12 to 15 months in the prior art, so the periods of aseptic sowing and rapid seedling are greatly shortened, and production efficiency is improved.

The invention discloses nutrient wheat germ powder and a preparation method thereof. The nutrient wheat germ powder is prepared from the following raw materials in parts by weight: 180-200 parts of wheat germ, 1.2-1.5 parts of potentilla discolor, 1.8-2 parts of rhodiola rosea, 2-2.5 parts of pericarpium citri reticulatae viride, 1.7-1.9 parts of notopterygium root, 2.5-2.8 parts of underleaf pearl, 1.8-2.2 parts of balsam pear rattan, 1.3-1.6 parts of philippine paphiopedilum, 7-8 parts of butter, 2-3 parts of powdered cinnamon, 3-5 parts of yellow wine, 10-12 parts of potato, 55-60 parts of sorghum rice, 4-5 parts of mung bean, 2-3 parts of water chestnut and 4-5 parts of nutrition additives. The wheat germ powder is good in mouthfeel; due to the addition of the sorghum rice and mung bean, faint scent is increased for the nutrient wheat germ powder, and the nutritive value of the nutrient wheat germ powder is improved. In addition, the nutrient wheat germ powder has the effects of clearing away heat and toxic materials, benefiting qi for activating blood circulation and improving eyesight and removing qi stagnation and is suitable for being eaten by all kinds of people.

The invention relates to cicerbita cyanea bathing powder capable of clearing heat and diminishing inflammation and a production method thereof. The cicerbita cyanea bathing powder comprises a medicinal powder bag and a spice bag, wherein the medicinal powder bag is prepared from the following raw materials in parts by weight: 35-45 parts of cicerbita cyanea, 10-16 parts of asarum petelotii, 10-16 parts of machilus leptophylla, 5-8 parts of justicia adhatoda, 5-8 parts of sageretia thea, 5-8 parts of mysore thorn root, 5-8 parts of rhaphidop hora hongkongensis, 3-6 parts of galium spurium, 3-6 parts of vittaria modesta, 3-6 parts of paphiopedilum and 3-6 parts of cissus hexangularis; the spice bag is prepared from the raw materials in parts by weight: 10-12 parts of rose, 8-10 parts of Chinese rose flower, 6-8 parts of bitter orange flower, 3-5 parts of lilac and 3-5 parts of lavandula angustifolia. The production method comprises the main steps: (1) preparing the medicinal powder bag, wherein the step of preparing the medicinal powder bag comprises the processes: (A) pretreating the raw materials; (B) soaking, evaporating to dryness, mincing and bake-frying; (C) extracting filtrates; (D) carrying out centrifugal separation and mixing solutions; (E) concentrating and drying; (2) preparing the spice bag; (3) loading the medicinal powder bag and the spice bag into a packaging bag, thereby preparing a finished product. The cicerbita cyanea bathing powder capable of clearing heat and diminishing inflammation, disclosed by the invention, has the effects of clearing heat and diminishing inflammation, resolving phlegm and removing stasis and reducing swelling and easing pain in long-term use.

The invention relates to the field of paphiopedilum planting related techniques and discloses an intelligent light intensity, temperature and humidity control system for paphiopedilum planting. The system comprises a client, a server, a planting management system and a central processing module. Bidirectional connection between the client and the server is realized through the Ethernet; bidirectional connection between the server and the planting management system is realized through the Ethernet; bidirectional connection between the planting management system and the central processing moduleis realized. The intelligent light intensity, temperature and humidity control system for paphiopedilum planting has advantages that light intensity, temperature and humidity are intelligently controlled by the system to guarantee the matching degree of the environment and paphiopedilum all the time, and the problem of influences on paphiopedilum growth due to paphiopedilum and growth environmentmismatching resulted from the fact that manual management in existing paphiopedilum planting wastes a great amount of labor and people fail to pay attention on and regulate the paphiopedilum growth environment all the time although a great quantity of modern tools are used is solved.