Tissue culture rapid propagation method of paphiopedilum markianum

Abstract

The invention discloses a tissue culture rapid propagation method of paphiopedilum markianum, belonging to the field of researches of plant tissue culture. The method comprises the following steps: (1) sterilizing explants; (2) carrying out germination culture on seeds; (3) carrying out multiplication culture; (4) carrying out differentiation culture; (5) carrying out rooting culture; and (6) carrying out acclimatization and transplanting. Paphiopedilum markianum capsules are collected at proper time; after sterilization, seeds are sowed into a sterile germination culture medium to germinate; different culture medium formulas are used for culturing at different culture phases, and then high-quality seedlings which are healthy and have stable inheritable characters are obtained by acclimatization and transplanting. According to the method, the propagation coefficient is high, the propagation period is short, the survival rate of transplanting is 92% and above, and large-scale commercialized seedling culture production can be carried out, so that the protection and development of paphiopedilum markianum wild resources are facilitated, and the application prospect is wide.

Description

technical field [0001] The invention belongs to the field of plant tissue culture research, and in particular relates to a method for tissue culture and rapid propagation of P. Background technique [0002] Paphiopedilum markianum Fowlie is a terrestrial or semi-epiphytic plant of the subgenus Paphiopedilum markianum Fowlie in the family Orchidaceae. It is a special species in China. It is produced in western Yunnan. It mostly grows on sparse forest trees and moss-covered rocks in limestone areas at an altitude of 1500-2000m. In or rocky areas, wild germplasm resources are very scarce. The leaves are narrow and oblong, and the flowers are large and light yellow-green. The flowering period is from June to August. It can be used in botanical gardens, potted plants, cut flowers or flower arrangements. It has unique ornamental value and However, due to habitat destruction, man-made destructive excavation and sale, tabby has become one of the endangered species in the world. It h...

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Problems solved by technology

[0003] Paphiopedilum is one of the most difficult orchids in tissue culture. There are very few research reports on Paphiopedilum tabby. The tissue culture of hybrid species is relatively easy, while the tissue culture of native species is difficult. Growing close to the soil, it is very difficult to cut out the explants, and it is very easy to be polluted by fungi

As there are many seeds in a single fruit of P. chinensis, aseptic germination and cultivation of seeds is a common method of propagation, but it is easily restricted by provenance. Due to the gradually decreasing population of P. chinensis, it is almost difficult to find natural ones in the wild. Healthy growing populations will face the threat of natural decline after several generations if seeds produced by a small number of individuals are used for long-term reproduction. At present, the method of using seeds as explants for tissue culture has not yet achieved breakthroughs

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