The present application belongs to the field of
agricultural biotechnology, and relates to a Hainan Catimor coffee
tissue culture breeding method, which takes the middle and upper section of the
hypocotyl of a Hainan Catimor coffee sterile
seedling as an explant, carries out, after sterile treatment, five stages of
callus induction, adventitious
bud differentiation, cluster
bud proliferation, rooting culture and
seedling raising and
transplanting in turn; each stage adopts a modified MS culture medium, and is matched with a special exogenous
hormone combination, and meanwhile, the culture temperature is controlled to be 20-25 DEG C, the suitable
light intensity and light time are controlled, and a specific concentration of PPM is added to inhibit
pollution. The present application has the advantages of fast induction speed, stable culture effect, compact and non-deformed
callus, healthy and neat adventitious buds and cluster buds, effective reduction of the proportion of abnormal seedlings and weak seedlings, significant improvement of the in-vitro regeneration efficiency and
tissue culture seedling quality of Hainan Catimor coffee, simple operation, strong
repeatability, suitability for large-scale factory breeding of Hainan Catimor coffee, and important application value.