The present invention relates to an efficient method for artificially synthesizing
Lactarius deliciosus
mycorrhiza, which comprises the following steps: (1) Take the
mycelium with a size of 1-3 mm at the connection of the stipe and cap of fresh
Lactarius deliciosus fruit bodies, place it in a PDA medium, and conduct
tissue culture at 20-25 °C for 20-30 days. Then, take the
mycelium block and inoculate it onto the PDA medium, and continue to culture at 20-25 °C for 25-40 days. Subsequently, take the
mycelium block again and inoculate it into a PDB medium for culturing for 30-50 days to obtain
Lactarius deliciosus liquid spawn; (2) Transplant pine seedlings at a density of 6-15 seedlings per basket into a culture basket filled with mycorrhizal substrate, add the
Lactarius deliciosus liquid spawn prepared in step (1) to the substrate 3-5 cm deep near the root of each
seedling, and
mycorrhiza can be formed after culturing for 10-14 days. The present invention scientifically screens the substrate and method for
Lactarius deliciosus. By using the optimized medium formula and culture method, the mycelium of
Lactarius deliciosus grows faster and the
biomass of the obtained Lactarius deliciosus mycelium is larger; inoculating the
plant roots with liquid spawn greatly improves the
inoculation efficiency; multiple inoculated
host plants are cultured in the same culture device, which greatly shortens the
mycorrhiza formation time after
inoculation and effectively improves the mycorrhiza synthesis rate; reducing the amount of
peat soil in the mycorrhizal substrate reduces the proportion of mycorrhiza formed by miscellaneous
bacteria and pine roots.