78 results about "Lateral root" patented technology

The invention relates to the technical field of soil improvement and ecological restoration, and discloses a liquorice planting method based on synergistic utilization of coal-based solid waste and desertified land, which comprises the following steps: crushing alkaline coal-based solid waste and acidic coal-based solid waste, mixing according to a mass ratio of 2: 8-4: 6, adding a modified humic acid-based heavy metal passivator and water, and uniformly mixing to obtain a mixture; uniformly mixing and aging to obtain a coal-based solid waste mixture; spreading the mixture on the surface layer of the desertification land, performing rotary tillage and deep tillage to mix the mixture with the sandy soil, and adjusting the pH value to be stable at 7.5-8.5; the treated liquorice seeds are sown, main roots are harvested in autumn of the third year, deep root systems and lateral root bud points are reserved, and overground straw is smashed and returned to the field; soil nutrition indexes are monitored regularly, other commercial crops are planted when the soil nutrition indexes reach a set threshold value, and if not, regeneration planting of the liquorice is maintained. Through synergistic compounding of acid-base solid waste and chelating of the passivating agent, substrate pH self-regulation and heavy metal resistance control are achieved, and the physicochemical property of sandy soil is improved.

This invention relates to a hydroponic root-inducing method for *Cinnamomum camphora*, characterized by the following steps: plant pretreatment; hormone-induced root treatment; perlite substrate for root induction transition; mist-controlled humidification environment for root induction; hydroponics; and disease prevention. The hydroponics includes: hydroponics in clean water until secondary lateral roots of a predetermined length are formed, followed by nutrient solution cultivation; the nutrient solution is supplied using a gradient approach. This invention's hydroponic root-inducing method employs a synergistic model of hormone-induced root induction, perlite fixation, and a high-humidity environment, significantly improving the success rate of root induction and avoiding the mortality problem caused by direct hydroponics. Through gradient acclimatization with nutrient solution concentrations, it significantly reduces the incidence of early root tip necrosis and root rot, improving survival rate and root vitality.

The invention discloses a fertilizer for promoting and improving the immunity of plants and a preparation method, and relates to the field of fertilizers. The preparation method comprises the following steps: mixing, smashing and grinding earthworms, eucalyptus leaves, wheat leaves and hawthorn leaves, putting into an ethanol solution, oscillating for 20-24 hours in a shaking table, and centrifugally filtering to obtain an active extracting solution; adding a sodium alginate solution into the suspended bacteria solution and the active extracting solution, and slowly adding carboxymethyl chitosan and a calcium chloride solution; and preparing. According to the invention, the plant growth-promoting rhizobacteria are fixed in the composite hydrogel, and the composite hydrogel can promote colonization of strains at plant root tips and lateral roots so as to regulate deposition of endothelial cell suberite, optimize mineral nutrition balance and abiotic stress response of plants and improve accumulation of auxin at the root tips; meanwhile, an active extracting solution is added into the composite hydrogel, immune response can be activated, and the metabolic activity and antioxidant activity of plants are greatly improved in combination with extracts of other substances.

The application provides a bamboo shoot seedling raising device and method, and relates to the technical field of bamboo shoot seedling raising, which comprises a supporting platform, the top end of the supporting platform is provided with a supporting frame, the top end of the supporting frame is provided with a connecting plate, and the periphery of the supporting frame is provided with a plurality of electric light curtains; a plurality of seedling raising baskets are detachably connected to the supporting platform; a plurality of lifting light supplementing mechanisms are arranged on the connecting plate, the lifting light supplementing mechanisms are connected with bamboo whip guiding mechanisms, and the bamboo whip guiding mechanisms are arranged correspondingly to the seedling raising baskets; and a spraying mechanism is arranged on the supporting platform. The seedling raising basket, low-nutrient light substrate and high-nutrient light substrate are used to avoid damage of the bamboo seedlings in the transportation process, and to improve the survival rate of transplanting. The top end advantage of the fast and robust growth of the whip tip is broken, the new bamboo whip is thickened in the high-nutrient light substrate, the number of lateral roots and the number of activated lateral buds are increased, and the quality of the nursery seedlings is ensured. The growth direction of the new bamboo whip is guided by the bamboo whip guiding mechanism, and the consistency of the nursery seedlings is improved.

The invention discloses an application of an OsCFIm68b gene in regulation and control of Cd resistance of rice and Cd accumulation of grains. The OsCFIm68b gene in rice is knocked out through gene editing to obtain a knockout mutant, and it is found that under the Cd polluted soil culture condition, the length of a main root is shortened, the number of lateral roots is reduced, the length of a total root is shortened, and the Cd concentration of grains is reduced to 1 / 3 of that of a wild type. Therefore, the OsCFIm68b gene is a negative regulatory factor for encoding Cd accumulation of the rice grains, the Cd accumulation of the rice grains can be improved by weakening the expression of the OsCFIm68b gene, the Cd resistance of the rice is improved, and a new gene target is provided for Cd low-accumulation breeding of the rice.

The invention discloses a populus euphratica PeXTH7 gene and application of the populus euphratica PeXTH7 gene in improving drought resistance of plants, and belongs to the technical field of gene engineering. The CDS sequence of the populus euphratica PeXTH7 gene is disclosed for the first time and is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2; the effect of the populus euphratica PeXTH7 gene in improving the drought resistance of the plant is disclosed for the first time, the root length and lateral root density of the plant are remarkably improved through overexpression of the populus euphratica PeXTH7 gene, and the drought resistance of the plant is improved by adjusting the root structure of the plant and promoting root development; the populus euphratica PeXTH7 gene provided by the invention can be used as a candidate gene for breeding the drought-resistant new variety of the plant, and is of great significance for cultivating the drought-resistant new variety of the plant by adopting a genetic engineering method, accelerating the breeding process and reducing the breeding workload.

This invention discloses a method for breeding salt-tolerant maize based on root function and artificial intelligence prediction, belonging to the field of crop breeding. It includes constructing a core germplasm resource bank, conducting high-throughput root phenotypic identification under 150mM NaCl stress, screening superior root systems based on quantitative thresholds such as a root-to-shoot ratio increase of more than 20% and lateral root density exceeding the mean by 1.5 times the standard deviation, performing dynamic multi-omics analysis at seven time points (0h, 3h, 6h) after stress to identify early response markers within 3-12h after stress, creating core parents by combining molecular markers and root traits, integrating seedling phenotypic, omics, and genotypic data, training an AI model with a prediction accuracy R² of no less than 0.65, and using the model to eliminate the last 50% of predicted potential materials in the early stages of breeding to guide parent selection. This invention achieves a paradigm shift from empirical screening to precise prediction, significantly improving breeding efficiency and directionality.

This utility model relates to the field of soybean planting technology, specifically disclosing a soybean planting box, including a base, a water collection tank fixedly connected to the upper end of the base, multiple planting box bodies arranged above the water collection tank, an adjustment mechanism arranged between the multiple planting box bodies, multiple evenly distributed conical protrusions arranged at the bottom of the inner wall of each of the multiple planting box bodies, and multiple drainage holes arranged alternately with the conical protrusions through the lower end of each of the multiple planting box bodies, and planting substrate filled in each of the multiple planting box bodies. By setting the conical protrusions at the bottom of the inner wall of the planting box body to form a three-dimensional slope, the main root of soybean is guided to grow down along the sloping surface of the protrusion, while the lateral roots extend laterally from the gaps between the cones, avoiding the root entanglement caused by the traditional flat bottom wall. The conical structure makes the bottom wall surface form a non-planar drainage channel, and excess water flows quickly to the surroundings along the sloping surface of the cone and is discharged from the drainage holes, preventing soybean roots from rotting due to waterlogging.

This invention discloses the application of the CLE11 small peptide gene in regulating plant salt tolerance, belonging to the field of biomaterials technology. This invention uses millet as the research material and clones... SiPROCLE11 Gene (CLE11 small peptide gene), construction SiPROCLE11 Overexpression and silencing of millet materials, exogenous application of the synthetic peptide SiCLE11p, and its role in enhancing millet salt tolerance were determined. Further investigation revealed that it can promote lateral root development and reduce Na+ in the roots. + This accumulation of knowledge laid the foundation for the discovery of salt-tolerant genes in millet and for salt-tolerant millet breeding.

The invention belongs to the technical field of plant tissue culture, and relates to a tissue culture and rapid propagation method for castanopsis sclerophylla. According to the method, castanopsis sclerophylla seeds are used as materials, and through sterilization method comparison, basic culture medium screening, hormone proportioning optimization and the like, an efficient tissue culture and rapid propagation technical system of castanopsis sclerophylla is established through seed sterile germination, bud proliferation, strong bud rooting and acclimatization and transplantation, and complete tissue culture progeny plants are formed. 100.0% of the seeds in the system germinate and sprout; the propagation coefficient of buds is large and reaches 6.8; the rooting rate reaches 92.8%, the root system is developed, the average is 4.1 roots per plant, the root length is 4.6 cm, and lateral roots are dense; the transplanting survival rate is high and can reach 95.2% or above; and the method is not limited by factors such as seasons, climate and regions, and can realize annual efficient and rapid propagation. According to the method, the blank of tissue culture and rapid propagation of the castanopsis sclerophylla is filled, a stable and efficient regeneration method for the in-vitro culture plant of the castanopsis sclerophylla is provided, large-scale seedling culture can be carried out, the cultured castanopsis sclerophylla seedlings are robust, neat and consistent, and a high-quality seedling guarantee is provided for improving the economic benefit of an artificial forest of the castanopsis sclerophylla.

The invention discloses a slope surface protection supporting structure of a high and steep soil slope and a construction method of the slope surface protection supporting structure, and relates to the field of slope supporting. S2, a traditional slope supporting structure is constructed, and the stability is detected; s3, digging a planting pit and carrying out wall protection treatment; s4, a root system control cage body is installed and fixed; s5, seedlings are planted and cultivated in the root system control cage body, and lateral roots extend into the soft soil body area in a directional mode; s6, the plants and the root system control cage are removed, and root system holes are protected; s7, a grouting anchoring assembly is arranged; s8, performing pressure grouting construction; s9, maintaining the grouting body and detecting the stability of the side slope; and S10, the planting pits are sealed and filled, and slope ending is completed. The problems that according to a traditional grouting technology, grout is difficult to permeate into a soft area in a soil body, and gaps are prone to being generated on the combination face of the grout and the soil body are solved, the grouting body can deeply permeate and be tightly combined with the soil body, and the overall construction technology gives consideration to the short-term ecological soil fixation requirement and the long-term engineering stability requirement.

The invention discloses a plant root system lossless seedling raising supporting device and a seedling raising method, and belongs to the technical field of plant seedling raising and root system research. The invention aims to solve the problems of root hair tearing and lateral root damage during seedling transfer or root system observation due to dependence on a solid substrate in the existing seedling raising technology. The device comprises a supporting base of an annular opening and closing structure, first supporting columns and second supporting columns which are alternately and obliquely arranged are arranged on the lower side of the supporting base and form a necking structure, supporting filter paper is arranged in the necking structure, and drainage filter paper making contact with the supporting filter paper is detachably connected to the supporting base; the seedling raising method is completed through device assembly, nutrient solution supply, seed cultivation, lossless seedling taking and device reuse. Seeds are supported in an auxiliary mode through a small amount of ceramsite on the supporting filter paper, nutrients are conveyed through the drainage filter paper, seedlings are taken out in a lossless mode in cooperation with the open-close type supporting base, it is guaranteed that the root system structure is complete, and the device is simple in structure, convenient to assemble, reusable and suitable for large-scale root system research and crop breeding and seedling raising operation.

The application provides application of RanGAP in regulation of symbiotic nodulation. A novel functional gene RanGAP (including RanGAP1 or RanGAP2) is disclosed, which encodes RanGAP protein with important biological function, and the protein is involved in symbiotic nitrogen fixation process of leguminous plants and rhizobium, and the application has significant meaning in genetic improvement of plant traits and soil improvement. An isolated promoter from RanGAP genome (upstream) is also disclosed, which has the function of driving gene-specific expression; more particularly, the promoter drives specific expression of the gene in root vascular bundle, lateral root / nodule primordium.

The application relates to the technical field of plant genetic engineering, and discloses application of a soybean low-phosphorus-tolerant gene GmAP2 in improving the low-phosphorus-stress tolerance of plants. The application finds that, under low-phosphorus stress, the expression level of the GmAP2 gene in plants is significantly up-regulated. By heterologously overexpressing the GmAP2 gene in Arabidopsis, the stress suffered by plants under low-phosphorus conditions can be effectively relieved, and the fresh weight and lateral root length of the Arabidopsis can be significantly increased, so that the growth of the Arabidopsis plant is promoted. In addition, under low-phosphorus conditions, overexpression of the GmAP2 gene can improve the phosphorus deficiency symptoms of soybean, improve the absorption of phosphorus nutrients by plants, and promote the growth of the roots and leaves of the plants. Therefore, the GmAP2 gene plays a very important role in the response of plants to a phosphorus-starvation stress environment.

The invention discloses an application of a corn ZmMYB165 gene and an encoding protein thereof in enhancing low-phosphorus stress tolerance of corn. The polynucleotide sequence of the corn ZmMYB165 gene is as shown in SEQ ID No: 1. Under a normal phosphorus supply condition, the number of lateral roots of a ZmMYB165 overexpression material and the number of lateral roots of a wild type material have no significant difference; after low-phosphorus treatment, the number of lateral roots of a ZmMYB165 overexpression material and the number of lateral roots of a wild type material are both remarkably increased, the number of the lateral roots of the wild type material is increased by about 16.39%, and the number of the lateral roots of a ZmMYB165 overexpression strain is increased by about 32.55%. Under low phosphorus stress, the biomass of the wild type overground part is obviously reduced, and the overground part of the ZmMYB165 overexpression strain is increased, which indicates that the ZmMYB165 overexpression material has stronger tolerance to low phosphorus stress.

The invention relates to the technical field of planting machines, in particular to a stevia rebaudiana and peanut interplanting planting machine and a planting method.The stevia rebaudiana and peanut interplanting planting machine comprises an equipment body, a plurality of rotating cylinders are rotationally connected to the equipment body, a pretreatment box is fixedly connected to the equipment body, and a plurality of seedling conveying pipes corresponding to the rotating cylinders are fixedly connected to the interior of the pretreatment box; a plurality of multi-section telescopic rods are fixedly connected to the interior of the pretreatment box, pretreatment pipes are fixedly connected to the ends of the multi-section telescopic rods, soil removing mechanisms are arranged on the pretreatment pipes, in the stevia rebaudiana seedling root hair pruning link, aged lateral roots longer than 5 cm can be accurately cut off through a cutter, invalid nutrient consumption is reduced, and the yield of the stevia rebaudiana seedlings is increased. The nutrient solution sprayed immediately after pruning can quickly stimulate healing of root hair cuts, the germination rate of new roots is increased, meanwhile, microelements and stress-resistant components are supplemented for root systems, and the tolerance degree of seedlings to adverse environments such as drought and slight salt and alkali is remarkably improved.

This invention discloses a peony PsLBD12 Genes and their application in regulating root development PsLBD12 The nucleotide sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence of its expressed protein is shown in SEQ ID NO.2. This invention was cloned from the peony cultivar 'Zhengwu'. PsLBD12 The gene sequence was obtained and its expression was analyzed in peony. Subsequently, an overexpression vector was constructed and introduced into the target plant to verify its function. It was found that overexpression... PsLBD12 Compared with wild-type Arabidopsis seedlings, the Arabidopsis seedlings with this gene grew faster, exhibited earlier stem elongation, and showed earlier senescence of basal leaves in the later stages of growth. The taproot length was significantly shortened, the number of lateral roots was significantly increased, and the endogenous hormones IAA and ABA in the leaves were significantly increased. The average number of roots in detached leaves was significantly increased on MS medium containing IAA, and the average root length was significantly increased on MS medium containing IBA. It is evident that this gene will have wide applications in the cultivation and genetic improvement of peonies and other horticultural plants.

This invention discloses the Anti-WOX11 small peptide from Aquilaria sinensis and its application in promoting plant root regeneration. Through genetic transformation, this invention obtained transgenic Aquilaria sinensis and Arabidopsis thaliana containing the Anti-WOX11 fragment. Experiments demonstrated that Anti-WOX11 can promote the regeneration of plant cuttings and the development of lateral roots. This invention fills the research gap on the direct regulation of cutting regeneration by small peptides in Aquilaria sinensis, providing a feasible solution for improving the survival rate of Aquilaria sinensis after root pruning and transplanting. It has great research potential and broad application prospects, and can be applied to promoting agarwood formation and the production and preparation of agarwood-related products.

The invention discloses a golden papaya tissue culture seedling culture medium block as well as a preparation tool and a preparation method thereof. The golden papaya tissue culture seedling culture medium block comprises a first base block and a second base block, the first base block is an agar base block, and the first base block is of a cylindrical structure; a filling material of the second base block is formed by mixing coco coir, perlite and vermiculite according to the volume ratio of 3: 1: 1, the filling material is subjected to high-pressure sterilization treatment, and the water content of the filling material is adjusted to 60%-70%; the second base block is of a cylindrical structure, a first base block containing groove is formed in the upper portion of the second base block, and the first base block can be inserted into the first base block containing groove; and the filling material of the second base block contains a root growth promoting agent. According to the culture medium block, the tedious operation of seedling taking, agar washing and transplanting in a traditional process is avoided, mechanical damage such as root tip breaking, root epidermis tearing and lateral root falling is fundamentally eliminated, and the tissue culture seedling transplanting survival rate can be remarkably increased.

The invention relates to the technical field of fertilization equipment, in particular to slow-release fertilization equipment for organic planting, which comprises at least two slow-release components capable of being spliced into a circular structure, and each slow-release component comprises an annular slow-release box, an inner side annular protective net, an outer side annular protective net, an annular fixing seat, a slow-release part and a liquid storage unit; the head and the tail of the annular slow-release box are connected through a detachable sealing structure to form an annular cavity adaptive to plant root system distribution; the lower end of the annular fixing seat is provided with a pointed or angular structure which is inserted into soil to be fixed and used for being correspondingly embedded into the bottom of an annular groove dug near a plant root system, and the annular slow-release box is supported on the ground and assembled with the annular fixing seat in an up-down corresponding mode to jointly define a containing space; according to the invention, through the at least two spliced slow-release assemblies, an annular structure matched with a radial range (generally 0.8-1.5 m from the periphery of a crown projection) of a fruit tree root system is formed, so that side roots and fibrous roots which are distributed in a radial manner can efficiently obtain water and fertilizer.

The application discloses application of ZmHB77 protein and a coding gene thereof in regulation of drought resistance of plants. The application discloses application of ZmHB77 protein (SEQ ID No. 1) or a related biological material thereof in all or part of the following: regulation of drought resistance of plants, regulation of seed root number of plants, regulation of lateral root density of plants, and the related biological material is a nucleic acid molecule capable of expressing the ZmHB77 protein or an expression cassette containing the nucleic acid molecule, a recombinant vector, a recombinant bacterium or a transgenic cell line. After gene knockout of the ZmHB77 gene drought resistance protein coding gene of the application, the drought resistance of corn is significantly improved. The application provides a more precise, efficient and safe technical method for creating a corn overexpression plant and / or corn breeding, realizes precise improvement of the drought resistance of corn, can promote the commercial corn breeding process, overcomes the short board of traditional breeding, shortens the breeding cycle and does not affect other properties.

The application belongs to the technical field of plant genetic engineering, and particularly relates to application of a PoPILS2a gene in promoting plant root development. The application finds that overexpression of the PoPILS2a gene can significantly promote the main root elongation and lateral root development of peony, and improve the overall root architecture, indicating that the PoPILS2a gene plays an important positive regulation role in the root development of peony. The application lays a solid theoretical foundation for future directional improvement of the peony root through genetic engineering means and improvement of the medicinal and ornamental values of the peony.

The application relates to the technical field of seed seedling raising, and provides a seedling raising method suitable for seeds of the genus Ormosia and application thereof, the seedling raising method comprises the following steps: (1) in early March, the seeds of the genus Ormosia are put into hot water with a temperature of 60-80 DEG C, the water is naturally cooled, the seeds are taken out after being absorbed, and the seeds are sprayed with water to keep moist; (2) step (1) is repeated once a day, and the process is continued for 5-7 days, so that the seeds are obtained after preliminary treatment; (3) the seeds after preliminary treatment are soaked in a suspension seed coating agent for 10-18 hours, so that the seeds after soaking are obtained; (4) the seeds after soaking are inoculated into a substrate to accelerate germination for 30-50 days. The seedling raising method can improve the germination rate and germination uniformity of the seeds of the genus Ormosia, and simultaneously improve the quality of seedlings, increase the height and the number of lateral roots of the seedlings. The seedling raising method is low in cost, wide in applicability, easy to popularize, and can provide reliable technical support for seedling raising of the seeds of the genus Ormosia of the legume family.

The utility model relates to the technical field of ginger aeroponic cultivation, in particular to ginger aeroponic cultivation and matrix cultivation combined cultivation equipment which comprises a box body, the interior of the box body is divided into a planting area on the upper portion and a liquid supply area on the lower portion, the planting area is specially used for ginger planting, and the liquid supply area is specially used for spraying and circulating treatment of a nutrient solution. A planting plate is arranged between the planting area and the liquid supply area, a planting part is arranged above the planting plate and used for planting ginger, an atomization assembly is arranged below the planting plate, and a nutrient solution can fully cover a root system area through the uniform spraying design that the atomization assembly surrounds a nozzle, so that stable nutrients and moisture are provided, and the planting effect is improved. A good high-oxygen growth environment is created for the root system, and a developed lateral root system can be formed, so that the growth of the root system of the ginger is promoted.

This invention discloses a method and application for promoting root bending and lateral root formation in seed-sown persimmon (Diospyros kaki). This invention belongs to the technical field of persimmon seedling cultivation and bonsai material cultivation. The method includes low-temperature stratification treatment of persimmon seeds; disinfection and imbibition treatment of the stratified seeds followed by sowing in a gibberellin-containing GA3 medium; periodic artificial adjustment of the radicle growth direction when the radicle reaches 1 cm in length to induce taproot bending; removal of 1 cm of root tip when the taproot reaches 4 cm in length to promote lateral root and fibrous root germination; and subsequent hardening-off and transplanting. This invention, by regulating early geotropism of the radicle and taproot apical dominance, enables the taproot of persimmon seed-sown seedlings to form a natural bending shape and significantly promotes lateral root germination, resulting in good ornamental value, growth vitality, and promising application prospects.

The application provides a rapid and high-quality breeding method of Vitis quinquangularis, which comprises six steps of selection and treatment of cuttings, disinfection of cuttings, root induction in an electric hotbed, preparation of a substrate, cutting seedling and transplanting. The single-bud pruning method of the application can obtain the most cuttings, the use of a compound solution of indole acetic acid and indole butyric acid as a rooting agent can make the taproot and lateral roots germinate at the same time, and the growth speed is increased by more than 50%, the application also uses a fermentation substrate nutrient bag for cutting seedling, which ensures balanced nutrition supply, the seedling rate can reach more than 98%, the survival rate can reach more than 95%, and the breeding cycle is short.

This invention relates to the field of plant root division technology, and more particularly to a root division method for root zone-root zone communication research and its application. The root division method of this invention includes the following steps: (1) Seedling cultivation: germinating seeds are hydroponically cultured to obtain seedlings; (2) Main root pruning of seedlings: when the lateral roots of the seedlings described in step (1) grow to 3-4 cm, the main root is pruned to obtain seedlings with pruned main roots; (3) Double rootstock grafting of seedlings: two seedlings with pruned main roots of the same growth are selected for double rootstock grafting to obtain grafted seedlings; (4) Grafting of grafted seedlings: the roots of the grafted seedlings are completely immersed in the culture solution, with the grafting interface outside the culture solution, and cultured until the grafting interface is completely healed and both rootstock A and rootstock B have grown new roots, to obtain seedlings to be divided; (5) Root division: the seedlings to be divided are transferred to a root division box for root division treatment. The root division method of this invention can more accurately and intuitively reflect the exchange of substances between roots.

This invention belongs to the field of fruit tree cultivation technology, specifically relating to the application of methyl jasmonate in improving nitrogen use efficiency in plants. Applying a certain concentration of methyl jasmonate can significantly improve the nitrogen use efficiency (NUE) of plants under limited nitrogen supply, specifically manifested in a significant increase in plant dry and fresh weight, total root length, total surface area, total volume, number of root tips, number of lateral roots, and root activity; an increase in plant photosynthetic capacity and nitrogen content, and a decrease in the carbon-to-nitrogen ratio. This indicates that an appropriate concentration of methyl jasmonate effectively improves the NUE of apple plants under limited nitrogen supply, promoting plant growth and laying the foundation for further research into the functional mechanism of methyl jasmonate in improving apple NUE. Furthermore, these findings may be applied in the future to guide production practices and improve the nutritional status of fruit trees.

The invention provides a pepper transgenic hairy root induction method which comprises the following steps: sterilizing pepper seeds, inoculating the pepper seeds to a germination culture medium, and culturing until cotyledons are completely expanded; cotyledons are taken and cut into segments, the segments are infected with agrobacterium rhizogenes K599 infection liquid containing exogenous genes after being pre-cultured, and the concentration OD600 of the bacterial liquid is 0.2-0.5; co-culturing after infection, and then transferring to an induction culture medium to culture for 2-4 weeks to obtain transgenic hairy roots; nAA can be added to regulate and control the shape of the hairy root, so that a main root extension type or a high-branch lateral root type is obtained. According to the invention, an efficient, stable and good-repeatability pepper transgenic hairy root induction system is constructed, and meanwhile, main root extension type or high-branching lateral root type hairy roots can be directionally obtained through NAA regulation and control. The method solves the problems of low induction efficiency and single scene in the prior art, provides a practical tool for pepper gene research, molecular breeding and metabolite development, and has theoretical and application values.

Disclosed is a use of a ZmRLR1 gene and an encoded protein thereof in regulating the development of a corn root system. By overexpressing the ZmRLR1 protein of a genetically modified plant, the number of lateral root primordia, the total length of lateral roots, and the total lateral root density of the root system in the seedling stage are all significantly increased, the number of root systems and the size of the root systems of the fifth layer of crown roots in the maturation stage are significantly increased, and the weight of the rhizosphere soil is increased.