526 results about "Lateral root" patented technology

The invention discloses sgRNA for promoting development of cotton lateral root, a target sequence of sgRNA is respectively composed of the nucleotide sequences shown in SEQ ID No.1 or SEQ ID No.2, and two target sequences can be used by combination. The expression of cotton argininase gene can be inhibited through a CRISPR / Cas9 gene editing system. Compared with wild type plants, the lateral root number of the gene editing plants obtained by silencing the cotton argininase gene is obviously increased, the root surface area is obviously increased, No content is increased, absorption and utilization of nitrogen element and other nutrients by cotton are increased, and sgRNA has important meaning for increasing the cotton fiber output and quality as well as increasing the cotton stress resistance.

The present invention discloses a guava quick propagation method which comprise the following steps: selecting daylight booth or green house of 70% transmittance, dipping cutting in rootage agent and inserting it to medium, keeping indoor relative humidity at 85%-100%, temperature at 25-40 degree, keeping indoor temperature at 25-35 degree after rooting, relative humidity at 75-85%, transplanting the cutting outside of green house for cultivation after the lateral root occur. The propagation rate is quick, rootage rate and transplanting survival rate is high in the present inventive method.

The invention discloses a method for promoting the culture of cyclobalanopsis glauca (Thunb.) Oerst. seedlings. Mixed medium of long lasting controlled release fertilizer is adopted, main roots and lateral root systems do not adopt air root cutting, the main roots and longer lateral root systems can pass through the bottom parts of a container and a seedling nursery plate to penetrate through the nursery ground for growth, well developed fibrous root systems are formed by manual cutting for many times, few lateral root systems can make series flow growth between containers, and the seedling success of the cyclobalanopsis glauca (Thunb.) Oerst. is greatly improved by the technical measures of seed wet sand stratification, disinfection, reasonable fertilizer, water, light irradiation, seedbed management and the like. The invention has the technical advantages of developed seedling fibrous root systems, prosperous growth vigor and convenient management, and has obvious difference from the conventional outdoor seedling culture and the like. The seedlings cultured by adopting the technology has high afforestation survival rate and transplanting survival rate and short transplant recovering period; and the invention effectively improves the quality of seedling afforestation and transplanting, has obvious benefits, and can be widely popularized and applied in production; and meanwhile the method also can be applied to the forcing culture of seedling of other tree species of fagaceae.

The invention belongs to the technical field of fruit trees and forestry cultivation, and discloses a method for promoting the lateral rooting of a annual carya illinoensis seed seedling, the root of a sprouting carya illinoensis seed seedling is mechanically cut, and a pair of scissors is utilized to cut the part below the fibrous root; and the seedling which is subjected to mechanical root cutting is transplanted into a nutrition pot, melon seed-shaped pebbles are laid on the bottom of the nutrition pot for 5 centimeters, and a nylon net is cushioned on the melon seed-shaped pebbles. A matrix is put in a container, the seedling is transplanted in a facility which is cold-proof and sun-proof for one month, a furrow which has the width of 13 centimeters and the depth of 20 centimeters is defined in a seedbed of a land, and the row spacing of the furrow is 30 centimeters. A layer of non-woven fabric is laid at the bottom of the furrow, a chemical rooting-resistant agent is coated on the non-woven fabric, and the nutrition pots which are planted with the seedlings are put on the non-woven fabric according to the planting distance of 20 centimeters, and are fixed finally. The water and fertilizer management period is normally carried out. The method can be used for effectively promoting the lateral rooting of the carya illinoensis seed seedling.

The invention provides an operation method for transplanting wild big trees, and relates to a method for transplanting wild big trees. The method comprises the following: a step of cutting off lateral roots, which is to take a trunk as a center and ditch taking the radius which is 3 to 4 times of the diameter of breast height, and a soil ball formed by ditching is in a reverse trapezoidal shape with big top and small bottom; a step of promoting new roots, which is to erect a row of wood boards serially connected by use of linen threads along a middle boundary of ditch width, spray root cuts with root power water on the side near the soil ball, spray the soil ball with root rot liquid medicine, backfill a mixed matrix of moss and river sand, backfill and tamp soil in layers on the other side; a step of cutting off a taproot, which is to ditch along the outer side of the wood boards after absorption roots are formed on the root cuts in next year, cut off a thick root and form a soil ball which is surrounded by the wood boards and is in a reverse circular truncated cone; a step of bunching the soil ball, which is to use straw ropes to bunch the basal part of a big tree and then nail guard plates; a step of lifting and unloading the big tree into a planting hole dug in advance after lifting and transporting the big tree to a destination; and a step of fixing and planting, which is to place the tree into the hole, remove the straw ropes and wood board binding materials and fill the hole with soil. The method can ensure that the survival rate of transplanted big trees is nearly 100 percent, thereby greatly improving environment greening ratio.

The invention belongs to the technical field of plant cultivation, and relates to a plant cultivation method, in particular to a cultivation method of broccoli. According to the greenhouse broccoli high-yield and high-efficiency cultivation technology, the disease resistant, medium-maturing and late-maturing broccoli with the long picking time is selected, seeds are sown, germination acceleration and poikilothermic treatment are carried out on the seeds, main root tips are cut off, growth of lateral roots are facilitated, and the seeds are cultivated in an open-mouthed rectangular container; meanwhile, by means of a nutrient solution, the seedling stage quality is good, the leaf color is dark green, the sound seedling index reaches more than 99%, planting is carried out when the seedling age is about 30 days, the seedling slowing time is short, the growth is fast, the ultimate capacity is high, the quality is good, the temperature inside a greenhouse can be adjusted by opening or closing large and small greenhouse thin films from the initial budding stage to the harvesting process of the broccoli particularly in autumn and winter which are low-temperature seasons, higher economic benefits can be obtained after the broccoli appears on the market before or after a festival, meanwhile, the quality of the broccoli is improved, the yield per mu can reach more than a ten thousand yuan, and the standardized pollution-free greenhouse cultivation is achieved.

The invention belongs to the technical field of fast propagation of plants, comprising the following steps of: (1) building a sterilized seedling bed on a selected propagation place, laying sterilized substrate in the seedling bed with sterilized cutting substrate of turfy soil, perlite and vermiculite concrete; (2) selecting healthy 1-2 years of seedlings with no damage by disease and insect or young trees as seed tree for collecting roots, digging a semicircular groove with a distance of 0.5m from the trunk, excavating the side root with the diameter of 1-3cm as propagation material and trimming the side root into root sections of 3-5cm; (3) soaking the trimmed root sections in carbendazim solution for sterilizing, adopting IBA+NAA solution as hormone, using ABT rooting powder solution to soak the base part for inducing the root; (4) inserting the root in the substrate of the seedling bed to lead the top end to be lower than the ground slightly and to be level with the ground after the soil is coated; (5) after cottage, covering the seedling bed with mulching films, sealing the periphery of the seedling bed, building tunnels on furrows and building overhead shade nets on the tunnels; and (6) after cottage, managing, transplanting and preventing damage by disease and insect.

The invention discloses a genetic engineering application of nitrate transport protein genome OsNRT2.3 of paddy rice, pertaining to the genetic engineering field. The nucleotide sequence of the nitrate transport protein genome OsNRT2.3 of paddy rice is SEQ ID NO.1, and OsNRT2.3a protein amino acid sequence expressed thereby is SEQ ID NO.2 and OsNRT2.3b protein amino acid sequence expressed thereby is SEQ ID NO.3. The gene of the invention is a first report on paddy rice. By participation in the transport of nitrate of paddy rice and especially under the condition short of nitrogen and poor pHenvironment, the analysis of mRNA expression shows that OsNRT2.3a is induced by low nitrate nitrogen so that the expression only occurs in lateral roots; and OsNRT2.3b has expressions in root system and parts over the ground and is responsible for the transport of nitrate. The OsNRT2.3a and OsNRT2.3b proteins are expressed in a frogspawn heterogenous system and determined as high compatible nitrate transport protein. A pH regulation and control site is found to exist in both proteins which are regulated and controlled by pH. Under low nitrogen condition, OsNRT2.3 transgenic plant shows higherefficiency in transport of nitrate, thus improving the utilization efficiency of nitrogen and the ultimate output.

The invention discloses a method for cuttage reproduction of a horseradish tree. Through preparation of matrix, preparation of rooting agent, section of cuttings, cuttage and disposal, after-cuttage management and protection, transplanting, and other stps, the cuttage reproduction of the horseradish tree are completed. By adopting the method provided by the invention, the cutting slips start to form a callus for about 10 days generally, and successively grow fibrous roots; after 4 weeks, about 80% of cutting slips take root. Compared with a routine method, when the cuttage reproduction of the horseradish tree is performed, the callus forming time of the root part of the cutting slips is short, the side roots of the cutting slips are multiple, and the root length is relatively long, an the rooting rate of cutting reaches 86% above. The method for cuttage reproduction solves the problems that it is difficult to take root in the cuttage process of the horseradish tree, and the root part is rotted for too long rooting time; besides, the cuttage survival rate is effectively improved.

The invention provides a lemon early bearing high grade high yield cultivating method, which effectively restrains growth of perpendicular roots of lemons and promotes robust extension of lateral roots and fibrous root through tilling, excavating planting ditches or holes, fertilizing base fertilizer, planting, sprouting and pinching and controlling water and fertilizer at the later time, thereby the overground portion of a plant forms large numbers of relatively short shoots whose young shoots are picked for a plurality of times through selection, short fruit branch groups are cultivated to use erasing redundant buds to replace the thinning out of branches and use pinching to replace lopping shears for effectively reducing loss of tree bodies, and short shoots are promoted by concentrating nutrition, ventilating and translucent conditions in fields are improved through reasonable space among plants, fruits in a tree body are evenly distributed from the inside to the outside and from the bottom up, which are convenient for closely planting, preventing diseases, controlling pests and managing water and fertilizer, the operation of covering bags for fresh fruits is more convenient. Trees begin to flower and bear fruits after being planted for 8-10 months, and the output can reach 200-300kg / acre, 1000-1500kg / acre in the second year and 2000-3000kg / acre in the third year, the manufacturing investment is reduced more than 30% compared with the traditional cultivating method, and the output of 4500-6000kg / acre can be harvested in advance for 3-4 years.

The invention aims to solve the problems in the prior art and provides a method for repairing root system injury of a rare old tree. In the method, the old tree serves as a receptor tree, the root system of the receptor tree and the root system of a donor tree are grafted, and the root system of the donor tree conveys nutrients, water and minerals to the root system of the receptor tree, so that the root system of the receptor tree can revive and rejuvenate. According to the position and degree of root system injury of the receptor tree, the repair plan may be single-lateral root repair, multi-lateral root repair or whole root system repair. By the repair method, the vitality of the old tree is restored and the old tree grows more vigorously. The method achieves the effect of in-situ saving and rejuvenation repair of old trees, helps to retain unique landscape marking significance and historical and cultural significance carried by the old trees, and is significant in scientific research values, ecological benefits, economic benefits and social benefits.

The invention relates to the plant gene engineering field, aiming at providing protein encoded by a rice root growth and development control gene OsSPR1 and a gene for encoding the protein. The protein has an amino acid sequence shown by SEQ ID NO:2 and a nucleotide sequence shown by SEQ ID NO:1. In the invention, a rice mutant with a short adventitious root and a short branch root is taken as a research material to determine that the gene is involved in regulating and controlling growth and development of the rice root; the functional deletion mutant of the gene shows reduced ferric content of blades, and the ferric content in the root is in accordance with that of wild rice; and recovery of expression of the gene can recover the ferric content in the blades, thus showing that the gene can regulate and control the ferric content in the blades. The invention provides a molecular regulation and control mechanism for rice root growth and development and ferric ion in-vivo transport, and provides a foundation for regulating and controlling a rice root structure and the ferric content by a genetic engineering method.

The invention discloses a watermelon planting substrate which is prepared by mixing a component 1 and a component 2. The component 1 is prepared from the following components in parts by weight: platycladus orientalis barks, field soil, a mixture of bentonite and vermiculite, mushroom residues and an inorganic fertilizer; the component 2 is prepared from the following components in parts by weight: masson pines, perlite, a mixture of attapulgite and kaolin, field soil and an inorganic fertilizer. The watermelon planting substrate disclosed by the invention not only is wide in source of raw materials, but also is low in cost. The substrate disclosed by the invention is prepared by independently preparing the component 1 and the component 2 and then mixing the two so as to provide a suitable environment for watermelon seedling cultivation, so that the survival rate of watermelon seedlings can be improved, and growth and development of the seedlings can be further promoted. By adding the treated mixture of bentonite and vermiculite, the loosening and ventilating performances of the substrate are enhanced. The substrate disclosed by the invention can be used for promoting development of a seedling root system, particularly development of lateral roots. Moreover, quick growth recovery and secondary development of the root system of the transplanted seedlings are facilitated.

The invention discloses a method for researching a partial root drying irrigation technology of woody fruit trees. The research method comprises the following steps of: manufacturing a root-zone root-splitting box, planting a tree seedling, performing partial root drying irrigation and the like. The root-splitting box is prepared from transparent organic glass or toughened glass with the thickness of between 2 and 3cm, the length, the width and the height of the box are all between 40 and 60cm, four small boxes in which no water can be leaked are uniformly partitioned by using a homogeneous material inside the box body, the bottom of each small box is provided with a small hole with the diameter of between 2 and 3cm, a main root of the tree seedling is cut off before the planting, and lateral roots are uniformly divided into four parts and are planted into the four partitioned small boxes; and the periphery of the root-splitting box is wrapped with a foam board with the thickness of between 8 and 10cm, and the research on partial root-zone fertilizer water is carried out after young sprouts of a fruit tree grow to 15cm. The method can be used for researching the dynamic influence of the supply space and consumption of fertilizer water of different rooting zone volumes of the woody fruit trees on the growth of tree bodies and root systems, and provides convenience for realizing the research on water conservation, fertilizer conservation, improvement on moisture utilization rate and the like.

The invention relates to a method for culturing wood seedlings by applying a culture box and belongs to a culture method of plant seedlings. The method comprises the following steps of: preparing a culture box by using expandable polystyrene with the density of 15kg / m<3>; inserting cutting bases into a nutrition bag filled by a filling machine; controlling the temperature to be below 38 DEG C and the humidity to be over 90 percent; moistening and cooling by using a negative-ion humidifier and moving windows; and culturing for 25 to 40 days to obtain greenhouse seedlings. By the method, the rooting ratio of the seedling reaches 94.0 percent, the seedling survival rate reaches 89.2 percent, and maturely-grow secondary lateral roots are generated. Compared with the original patent named three-dimensional seedling culture method of nursery stock and product thereof, the culture box is lighter and is easy to operate, the working efficiency is improved due to the application of mechanical equipment, and low investment and high utilization ratio of facilities are realized. Compared with the traditional plane seedling culture, the seedling quantity per unit area is improved by 5 times, the cutting survival rate is improved by over 35 percent, and water is saved by over 80 percent.

The invention discloses a rapid cutting propagation method of crape myrtle single-plant germplasm. On the basis that crape myrtle single plants with the good characters of the natural tree type, flowering habit, flower color and flower diameter size and the like are surveyed and screened out, incising and sprouting promotion is performed on optimized single-plant rootstock portions or purging and sprouting promotion is performed on the surface soil layer lateral roots to form young root sucker coppice shoots at the developmental stage, the coppice shoots are used for ear making and cutting propagation, cutting seedlings are used for building a cutting orchard, cutting orchard building and management are performed by routine, scion wood is completely collected and processed on the base of the trunk of the stock plant to promote sprouting and cultivation of coppice shoots, the standard quality of ear cutting and making is strictly regulated, the cutting seedbed matrix arrangement is simplified, rooting accelerator arrangement and using method are optimized, limitation of the cutting season is broken through, rapid long-term large-scale cutting seedling is achieved, the cutting survival rate and nursery stock quality are improved, and the cutting seedling cost is lowered.

The invention discloses multi-lateral root fruit tree bridging technology, which is a method for performing single-head bridging on a one-year high-quality seedling of a pyrus betulaefolia rootstock which is transplanted successfully on a plurality of sides, 50 to 70 centimeters away from the ground surface, of a fruit tree trunk when a fruit tree is in a growing season. By bridging the fruit tree by the method, the rootstock and the high-quality seedling have no impedance response basically; the root is supplied with sufficient nutrition so that the fruit tree obtains increased nutrients; therefore, harvest rate and fruit quality are improved; and the disease resistance of the fruit tree is improved and a survival period is prolonged.

The invention discloses a protein coded by a rice lateral root control gene OsIAAII, which has an amino acid sequence represented by SEQ ID No.2. The invention also discloses the gene coding the protein, which has a nucleotide sequence shown by SEQ ID No.1. The gene of the invention can be used for building transgenic rice having a rice lateral root control function. As the normal germination andgrowth of lateral roots are critical to the maintenance of the growth and high and stable yield of rice and the gene only affects the germination and growth of the lateral roots, the application potential of the gene in molecular breeding is high.

A fermented brown coal residue soil conditioner is prepared by evenly mixing brown coal residues, brown coal essence liquid, medical stone, composite bone meal, dry sapropel, and a composite microorganism inoculant according to a certain weight ratio and then fermenting the mixture. The absorbing performance and salt-base exchange performance of the soil conditioner are strong, the soil conditioner can improve the soil breathability, the crop respiratory effect is improved, the absorbing performance of roots is enhanced, the lateral root growth is promoted, the crop photosynthetic effect is effectively improved, the nutrients for plants are balanced, and the growth is promoted. The wastes (brown coal residues and brown coal essence liquid), which are generated during the brown coal processing process, are utilized, the wastes are converted into valuable resources, the product addition value is increased, an income increasing method is developed for humic acid producing entrepreneurs, a novel thinking is provided for developing soil conditioner, and the soil conditioner eventually improves the soil quality and increases the economic profits.

The present invention pertains to the agricultural technical field, relating to the application of sodium nitroprusside in drought resistance of crops. Sodium nitroprusside is confected into 10 to 30mg / L aqueous solution and then irrigated into roots or sprayed on leaves of crops. The crops processed by sodium nitroprusside in the seedling period can obviously improve the drought resistance, and after one week of natural drought, the water content still maintains high and the growth is recovered after drought rehydration. In addition, sodium nitroprusside can significantly promote the growth of wheat lateral root and plays an important role in improving the plant-root water absorption.

The invention discloses a sweet corn lodging-resistance seedling raising and culturing method including steps of (1) applying fertilizer and preparing soil trays, (2) sowing seeds in the trays directly, (3) performing root excision after seedlings grow neatly, (4) performing ventilative acclimatization to the seedlings before transplanting, (5) planting green manure of vetch, (6) plowing the vetch into fields, and (7) transplanting, watering roots, ridging and then covering mulches. The method has the advantages of simplifying seedling raising and culturing processes, saving time and labor and the like, further, enabling roots of sweet corn to grow strongly, increasing growth speed of aerial roots, improving effective fertility of plant soil and soil permeability, reducing field weed effectively, improving quality of sweet corn effectively and increasing output by 15-20% as compared with that of the conventional culture method. By means of seedling root excision, soil humidity at roots is reduced, growth of lateral roots and aerial roots is accelerated, the root system total is increased by 10-25%, and thus, lodging resistance capacity of sweet corn is improved.

The invention belongs to the technical field of garden planting, and particularly relates to a high-survival rate planting method of taxus chinensis. The high-survival rate planting method comprises the following five steps: performing field conditioning, digging holes and preparing a field, selecting seedlings, planting, and performing water and fertilizer management, wherein in the field conditioning process, an organic fertilizer is early applied so as to ensure the organic matter content and the microorganism content in soil in the planting process; in the seedling process, roots are uniformly adsorbed with sterol substances in rapeseeds soaked by rapeseed soaking liquid, growth and development of the roots in the planting process can be effectively promoted, germination of side roots is promoted, and the survival rate of the taxus chinensis in the planting process is improved; in the planting process, the moisture content of the soil is strictly controlled, the fact that the soil is not too dry nor too wet is ensured, and the taxus chinensis has a relatively strict requirement on moisture in the growing process and is not resistant to waterlogging, so that in the planting early stage, strict control over the moisture content of the soil can satisfy the growth need of the taxus chinensis to the maximum extent, the survival rate is ensured, and a root rotting phenomenon is avoided. The taxus chinensis planted by the high-survival rate planting method has the survival rate higher than 98%.

The invention discloses an alfalfa drought tolerance identification method, comprising the following steps: after sterilization on seeds, carrying out a conventional sprouting experiment, after a seedling has 3-4 main leaves, performing thinning and final singling, reserving materials whose plant size and height are roughly consistent, and carrying out a water controlling experiment in a greenhouse; in the 8th-11th day, taking the plants out to count the number of roots of each plant of alfalfa, including the number of main roots, lateral roots, and fibrils on the lateral roots; after processing for 20 d, respectively measuring seedling height, fresh weight of root systems, dry weight of root systems, leaf chlorophyll contents, stomatal conductance, leaf free water content, leaf surface bound water content, proline content and root volume of each processing group; performing data reduction, calculating average values, and calculating a drought tolerance coefficients and a comprehensive drought tolerance value of each variety; and determining salt tolerance of the varieties according to the comprehensive drought tolerance value. The method performs multivariate statistical analysis on agronomical characters and indexes of an alfalfa seedling stage, so as to perform comprehensive judgement and evaluation on the drought tolerance of alfalfa.

The invention discloses a tissue culture and regenerated plant in vitro mycorrhization method for pinus massoniana, which belongs to the technical field of biotechnology and modern forestry. The method comprises the following steps of: taking a terminal bud of an aseptic seedling of the pinus massoniana as an explant, inducing to produce a large quantity of multiple shoots with strong growth first, further inducing the generation of an adventitious root, transferring a plant with the macroscopic adventitious root onto a perlite substrate under the condition of asepsis after the induction of the adventitious root is finished, and inoculating ectomycorrhizal rungi Pisolithustinctorius at the same time to realize the mycorrhization in a bottle. The mycorrhization improves the quality of a root system of the adventitious root of a tissue culture regenerated plant of the pinus massoniana, and ensures that the average lateral root number and the average root length are greatly improved. The mycorrhization also makes the state of the adventitious root greatly changed, and a fungal mantle obtained by closely arranging more than ten layers of mycelia is formed on the surface of the adventitious root. Through the method, the induction rate of the multiple shoots of the pinus massoniana reaches 95 percent, the proliferation rate reaches 430 percent, the rooting rate reaches 85 percent, and the transplantation survival rate is improved to more than 85 percent from 65 percent.

The present invention provides a method for separating out rice root xylem parenchyma cell protoplasts. The method chooses the part of the extended and the mature sections of a rice Oryza sativa L. seeding root, which is 2cm to 3cm long and does not has lateral roots grown, uses hydrolytic enzyme liquids with different ingredients and finds the optimal separation conditions to successfully separate out the intact stele xylem parenchyma cell protoplasts. At present, the related report is not published yet at home and abroad.

The invention discloses a rooting-promoting, disease-resistant and plant-strengthening agent for cotton and a water, fertilizer and pesticide integrated application method. The rooting-promoting, disease-resistant and plant-strengthening agent is mainly prepared from a growth regulator, a microelement, a signal molecule donor and an elicitor capable of improving the disease resistance of the cotton, wherein the growth regulator is prepared from indolebutyric acid, sodium nitrophenolate, naphthylacetic acid and diethyl aminoethyl hexanoate through compounding, the microelement is prepared from zinc sulfate, ferrous sulfate, ammonium molybdate, amino acid powder and ammonium hydrogen phosphate through compounding, the signal molecule donor is NO donor sodium nitroprusside, and the elicitor is a penicillium dry mycelium extracting solution. The problems that a drip irrigation technology does no good to root development and severe diseases are caused by successive cropping to the cotton are solved, the growth regulator accelerates growth of root systems, the physiological function time of the root systems is prolonged, the disease-resistant elicitor enhances the resistance of cotton plants, growth of lateral roots is further promoted in combination of the signal molecule donor, and the disease-resistant response of the cotton plant is promoted. The rooting-promoting, disease-resistant and plant-strengthening agent can promote growth of roots and resist diseases in multiple angles; the agent can be injected into irrigation water through a fertilizer pump, integrated application of water, fertilizer and pesticides in the drip irrigation cotton field of the northwest inland is realized, and the agent can promote growth of roots, resist diseases and strengthen seedlings and has remarkable disease resisting and yield increasing effects.

The invention discloses a non-woven light-matrix sowing seedling breeding method for firs. The non-woven light-matrix sowing seedling breeding method for firs mainly includes steps that mixing coco coir, peat soil and water-retaining agent to prepare culture matrix, and preparing into a nutritious cup through a non-woven light-matrix forming machine; soaking in 0.1-0.5% potassium permanganate solution for 30 to 60 minutes before seeding, using clean water to clean, soaking the seeds in 40 to 50 degrees centigrade hot water, cooling naturally, changing water and soaking for 24 to 48 hours, and accelerating germination in a germination accelerating machine at 25 to 30 degrees centigrade after using clean water to clean; when the seeds expose white, seeding in the light-matrix nutritious cup, and covering a piece of film; after the lateral roots of the seedlings pass through the cup, fertilizing according to the time interval of 10 to 20 days, when the seedlings are 5 to 10 centimeters high, grading to a seedling tractor, exercising seedlings at the end of November, wherein the seedlings can be sold after the exercising. The non-woven light-matrix sowing seedling breeding method for firs has advantages that roots of the fir seedlings cultured on the light-matrix nutritious cup are flourishing, the nursery plants are strong, the matrix is light so that the transportation cost and forest planting cost can be lowered, and the matrix which cannot enable the plant to survive can be recycled; a constant-temperature germination accelerating box is high in germination accelerating rate and good in germination consistency and effectively improves the germination rate of fir seeds.

A water-saving method for planting tree includes such steps as pouring water in a container, sealing it, burying it in a dug pit, compacting the soil but exposing the top of said container, pricking a small hole on the top of said container, and conventionally planting a tree in the pit but inserting the lateral root of tree in said hole. Its advantages are low cost, saving water and high survival rate.

The invention relates to a biological agent type rooting liquid for promoting plant to root, and a preparation method thereof. The rooting liquid comprises 20-140 g / L of Bacillus natto powder, 10-70 g / L of potassium humate, 3-18 g / L of chitosan, 10-70 mg / L of single nitrification guaiacol sodium, and 20-100 mg / L of fatty alcohol polyoxyethylene ether. According to the invention, the rooting liquid is prepared by using scientific biological formula, and is rich in special biological agents and organic ingredients, can strengthen growth of taproots, promote germination of lateral roots, form a large root system; inhibit growth of pathogen, promote heal of wound, enhance resistance of trees; control soil compaction, promote absorption of nutrient, improve soil environment, and thus increase survival rates of nursery stocks. The rooting liquid is convenient to use, few in amount and quick in effects, and thus is a high-efficient and environmental-protective biological agent type rooting liquid.

The invention discloses a method of utilizing taxus lateral root proliferation to produce paclitaxel, the steps are that: (1) annual or biennial taxus branches are selected as cutting materials; (2) after the cuttings are sequentially injected into KMnO4 solution, sucrose solution, and plant growth hormone solution for soaking, the cuttings are conveyed to cutting substrate; (3) the cuttings are pulled out after the roots grow up, and are continually conveyed to the cutting substrate after being processed with hormone; (4) a certain quantity of the lateral roots are cut for producing the paclitaxel; (5) being dried under temperature control at the temperature that is less than or equal to 55 DEG C, pure products of the paclitaxel are obtained after processes of grinding, concentrated organic solvent extraction, column chromatography, solvent recovery, crystallizing and drying; or the cut taxus lateral roots are cultured in a improved Murashige and amp;Skoog fluid nutrient medium, bacteriostat is added in the nutrient medium, 2 to 9 days after fresh taxus lateral roots are induced and processed by elicitors, the pure products of the paclitaxel are extracted. The invention provides a new approach for medicine sources of the paclitaxel, and the shortcomings in artificial cultivation of the taxus are solved of low rooting rate, low survival rate after the roots are cut, slow roots proliferation speed, etc.