215 results about "Lateral root" patented technology

The invention discloses a salt-tolerant growth-promoting bacillus cereus strain and an application thereof. In particular discloses bacillus cereus T146, and the preservation number of the bacillus cereus T146 is CGMCC (China General Microbiological Culture Collection Center) No.30429. The bacillus cereus T146 disclosed by the invention has good biofilm production, siderophore secretion and nitrogen fixation capabilities, and can remarkably increase the root length, the overground dry weight, the plant height, the number of lateral roots and the average density of the lateral roots of wheat under the condition of salt stress. According to the invention, a low-cost, efficient and applicable culture medium aiming at the bacillus cereus T146 is determined, a stable and controllable fermentation system is established, the preparation method of the microbial inoculum with high mature spore number is obtained by optimizing a spray drying process, and the active spore number of the microbial inoculum can reach 1.3 * 10 < 11 > CFU / g. The bacillus cereus T146 has good development and application prospects in the fields of microbial agents, microbial fertilizers, soil improvement and the like.

The invention relates to the technical field of plant genetic engineering, and discloses application of a soybean low-phosphorus-tolerant gene GmAP2 to improvement of low-phosphorus stress tolerance of plants. Research finds that under the condition of low phosphorus stress, the expression level of the GmAP2 gene in plants is remarkably increased. The GmAP2 gene is subjected to heterologous overexpression in arabidopsis thaliana, so that stress on plants under a low-phosphorus condition can be effectively relieved, the fresh weight and lateral root length of the arabidopsis thaliana can be remarkably increased, and the growth of the arabidopsis thaliana plants is promoted. In addition, under the low-phosphorus condition, overexpression of the GmAP2 gene can improve the phosphorus deficiency symptom of the soybean, improve the absorption of phosphorus nutrients by plants and promote the growth of plant roots and leaves. Therefore, the GmAP2 gene plays a very important role in dealing with the phosphorus starvation stress environment by the plant.

The invention relates to the technical field of molecular biology, and discloses application of TaPSY5 small peptide and a coding gene thereof in promoting plant root growth. A tyrosine sulfated polypeptide is named as TaPSY5 small peptide and is as follows: (a) mature small peptide composed of 18 amino acids shown in a sequence 1 in a sequence table and having tyrosine sulfated modification; the TaPSY5 small peptide precursor protein is the following (b) or (c): (b) a protein composed of amino acids as shown in a sequence table 2; and (c) a protein derived from the amino acid of the sequence 2 in the sequence table through deletion / or substitution / or insertion of one or more amino acids. The TaPSY5 small peptide is externally applied, so that the growth rate of a plant root system can be increased. The gene (TaPSY5 gene) for coding the TaPSY5 precursor small peptide also belongs to the protection range of the invention. The invention also protects that the TaPSY5 gene is introduced into a target plant, and lateral roots and main roots of the obtained transgenic plant are significantly more than those of the target plant.

The invention belongs to the field of muskmelons, and particularly relates to a muskmelon seedling stage root system premature ability evaluation method. According to the melon seedling stage root system early emergence capability evaluation method, black soil is used as a medium to cultivate plant seedlings in batches, image analysis and data acquisition are facilitated, the measured main root length, lateral root total length and lateral root number variation range distribution is uniform and continuous, variation difference is remarkable, and the method is suitable for large-scale popularization and application. The difference of root growth conditions of different plant materials can be reflected. The evaluation method provided by the invention can be used for evaluating the early germination capability of different muskmelon materials, so that the experiment time is saved, the experiment repeatability is high, the stability is high, the data reproducibility, repeatability and stability are relatively good, the evaluation of the early germination capability of the root systems of a plurality of muskmelon materials can be completed at one time, and the evaluation method has a wide application prospect. The high-throughput evaluation of the early-growing capability of the melon root system is realized.

The invention provides tomato straw carbon dots as well as a preparation method and application thereof, and belongs to the field of nano technologies and nano materials. The method comprises the following steps: crushing tomato straws, adding water according to a mass ratio of 1: (90-110), carrying out ultrasonic treatment, and heating at 130-200 DEG C for 6-10 hours; after centrifugation, filtration and dialysis, freeze drying is performed, and the tomato straw carbon dots are prepared. The tomato straw carbon dots are used as a root promoting agent or a plant growth auxiliary agent, the germination potential, the germination rate and the number of radicles and lateral roots of muskmelon seeds can be effectively increased, the root system of muskmelon seedlings is promoted to be more robust, and the plant growth vigor is more vigorous. In a saline-alkali soil environment, the tomato straw carbon dot aqueous solution can also be used for treating melon seeds to obtain a good effect, melon root systems can reach the growth level in non-saline-alkali soil, and the effect is remarkable.

The invention discloses a growth-promoting rhizosphere bacterium streptomyces cerevisiae QS9-7 and application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of Streptomyces setonii QS9-7 is obtained through separation, and the preservation number of the Streptomyces setonii QS9-7 is CGMCC (China General Microbiological Culture Collection Center) No.32329. Experiments show that the strain QS9-7 has a relatively strong inhibition effect on various plant pathogens, and shows excellent biocontrol bacterium potential. The strain can effectively improve radicle length, germ length, lateral root number and germination rate of plant seeds and plant height and stem diameter of plant seedlings, obviously promotes plant growth, and has high application value in green prevention and control of plant diseases.

The invention provides an asiatic toddalia root twig cutting seedling raising method, and relates to the technical field of asiatic toddalia root artificial planting. The Asiatic toddalia root twig cutting seedling raising method mainly comprises the steps of selecting cutting branches, preparing a soaking solution from a 0.05% boric acid solution, IBA and DL-malic acid, treating the upper ends of the cutting branches with an ice-water bath soaking solution, treating the lower ends of the cutting branches with a normal-temperature soaking solution, soaking the cutting branches with quicklime mixed cooked rice milk and the like. According to the method, the defects in the prior art are overcome, the branch cutting rooting rate is effectively increased through treatment of the cutting branches, meanwhile, growth of lateral roots and fibrous roots is promoted, and the quality of Asiatic toddalia root cutting seedlings is improved.

The invention discloses a ZmLG3 gene and application thereof in regulation and control of nitrogen efficiency and lateral root number, and belongs to the field of plant biotechnology breeding. According to the corn ZmLG3 gene provided by the invention, after the endogenous gene generates function loss mutation in a plant or the expression of the ZmLG3 gene is inhibited, phenotypes with improved nitrogen utilization efficiency and / or increased lateral root number can be obtained, and the gene resource and the application method thereof have wide application prospects in the field of corn breeding.

The invention provides a vegetable seedling and seedling strengthening microbial composition, a substrate and a preparation method, and belongs to the technical field of microbial agents. The invention provides a microbial composition for vegetable seedling culture, the microbial composition comprises a bacterial liquid of various bacteria, the types of the bacteria comprise bacillus subtilis, bacillus amyloliquefaciens, paenibacillus polymyxa and azospirillum brazilianum, and the bacteria have a synergistic effect, so that the seed germination time can be remarkably shortened, and the root length and the lateral root number can be increased. According to the invention, the microbial composition is used as a basis, the microbial composition, mushroom wastes, straws, fresh livestock and poultry manure and the like are subjected to closed fermentation to prepare the matrix for vegetable seedling culture, seeds can be directly planted in the matrix for vegetable seedling culture, the seed germination time can be further shortened, and meanwhile, statistics is carried out within the same time after germination, and the result shows that the seed germination time is greatly shortened. Seedlings cultivated by using the matrix for vegetable seedling cultivation are more robust, and particularly, stem bases are thicker, leaves have larger surface areas, and the color is darker green.

The invention discloses application of a PdeTLP protein and a related biological material thereof in regulation and control of plant root system development. Experiments prove that compared with wild poplar, the number of adventitious roots, the number of adventitious root lateral roots, the root length and the root included angle of PdeTLP knockout poplar are obviously increased, and the plant height, the number of stem nodes and the stem diameter are also obviously increased. In addition, the xylem width, the phloem width and the xylem cell area of the poplar with the PdeTLP knocked out are obviously higher than those of the wild poplar. The PdeTLP gene participates in regulation and control of the root system number, the root system length, the root system included angle, the plant height, the stem node number, the stem diameter and the secondary microtubule tissue development of the poplar. The method not only has important significance on molecular mechanism and theoretical research of regulating and controlling the number and length of poplar root systems, plant height, number of stem nodes and stem diameter, but also has important guiding significance on directionally cultivating trees meeting actual production requirements.

The invention discloses application of a gene MtPrx38 in regulation and control of growth, development and quality of forage grass. The method comprises the following steps: carrying out genome PCR (Polymerase Chain Reaction) identification to obtain homozygotes MtPrx38-1 and MtPrx38-2 of the medicago truncatula MtPrx38 gene mutant; the expression quantity of the MtPrx38 gene detected by RT-PCR (Reverse Transcription-Polymerase Chain Reaction) is obviously reduced, and compared with wild medicago truncatula, the peroxidase activity of the mutant homozygotes MtPrx38-1 and MtPrx38-2 is obviously reduced. Compared with wild medicago truncatula, the MtPrx38 gene mutant has the advantages that the root length, the lateral root number, the plant height, the branch number, the leaf area and the secondary branch number are obviously increased; the flowering time is advanced; the lignin content and the cellulose content are obviously reduced, which indicates that the gene MtPrx38 has the functions of regulating and controlling the growth, development and quality of forage grass. The method has application prospects in the aspects of promoting growth and development of forage grass, improving forage grass quality and the like.

The invention relates to a small molecule peptide, a plant growth regulator containing the small molecule peptide and application of the plant growth regulator, and belongs to the technical field of plant peptide hormones. The small molecule peptide is a plant PIP family small molecule peptide, and the amino acid sequence of the small molecule peptide is RFVKHSGP (OH) SPSGPGH. The small molecule peptide can effectively promote the growth of lateral roots of plants, can be used as a plant growth regulator for promoting the growth of the lateral roots of the plants, and is suitable for agricultural application.

The invention belongs to the technical field of agriculture, and particularly relates to a fertilization method for improving the quality and yield of oat seeds, diammonium hydrogen phosphate and biochar are used as base fertilizers, the application amount of the diammonium hydrogen phosphate is 10-12 kg / mu, the application amount of the biochar is 10-15 kg / mu, bacterial fertilizer dressing is carried out in the seedling emergence period of crops, and chemical fertilizer dressing is not carried out in the later period; the bacterial fertilizer is an arbuscular mycorrhizal fungus inoculant and a trichoderma inoculant. The use amount of the chemical fertilizer is reduced, and the negative effect of excessive use of the chemical fertilizer on crop growth is reduced; the soil environment is improved by additionally applying biochar, and the nutrient utilization efficiency is improved; by utilizing a hypha network formed by arbuscular mycorrhizal fungi and root systems, the absorption area of the oat root systems is increased, and the nutrient utilization efficiency of plants is improved; the application of the trichoderma can promote the development of lateral roots of plants and accelerate the growth speed of the plants in the seedling stage; the fertilization method can improve the quality and yield of the oat seeds, is simple and easy to implement, is suitable for large-scale popularization and application, and has a wide market prospect.

The invention relates to the technical field of plants, in particular to application of surfactin in promotion of rape root development. According to the invention, a new function of the surfactin is found, and the surfactin can be used as a signal molecule to promote expression of related genes of auxin of the rape and promote development of lateral roots of the rape and formation of a branch prosite, so that development of a root system of the rape is remarkably promoted. Surfactin can be used for preparing products for promoting root development of rape, such as plant growth regulators, fertilizers or fertilizer additives and the like, and has a wide application prospect.

The invention provides a root development monitoring device for pasture growth, and relates to the technical field of pasture monitoring, and the root development monitoring device comprises a planting frame, a placement plate, a cultivation assembly and an inspection mechanism; a water culture box is erected above the placement plate, the culture assembly comprises a partition plate fixedly arranged in the middle of the water culture box, four inner lining plates are fixedly arranged on the inner wall of the water culture box and located on the two sides of the partition plate, and water culture trays are placed on the upper surfaces of the four inner lining plates. According to the scheme, a transparent observation frame is finally arranged in the middle of the water culture box, and a bidirectional camera capable of adaptively ascending and descending along with the shapes of the inclined planes on the two sides of a limiting plate is arranged for observing the transparent observation frame; through the transparent observation frame, the development conditions (the shapes of main roots and lateral roots, the growth conditions of the lateral roots on the main roots, the growth degree of the main roots, whether the root systems decay or not and other conditions) of the root systems of the forage grass in the water culture box can be visually observed.

The invention belongs to the technical field of biology, and discloses a wheat lateral root length and drought resistance related STS marker and application thereof. The invention provides an application of an InDel molecular marker, the InDel molecular marker is a DNA fragment from the 84th site to the 103rd site of which the nucleotide sequence is SEQ ID NO: 1, the application is to identify or assist in identifying the lateral root length and / or drought resistance of wheat, and the InDel molecular marker can be used for wheat breeding. The STS molecular marker provided by the invention can provide a strong selection site, improve the accuracy of selection of breeding materials with lateral root length and drought resistance related traits, and realize the target of molecular marker-assisted selection of excellent wheat varieties.

The invention discloses a culture method for promoting germination of cercis chinensis seeds and growth of seedlings, which comprises the following steps: accelerating germination: soaking the cercis chinensis seeds in a seed soaking solution at normal temperature to treat for 3-4 hours, accelerating germination of the soaked cercis chinensis seeds for 5-9 days, and taking the germinated cercis chinensis seeds; sowing: dibbling the germinated cercis chinensis seeds in a sowing matrix, covering the seeds with 1-2cm of the sowing matrix, keeping the moisture of the sowing matrix at 50-65%, and separating cercis chinensis seedlings with 1-2 true leaves from the sowing matrix when the cercis chinensis seeds grow 1-2 true leaves; seedling cultivation: transplanting the 1-2 true-leaf cercis seedlings into a cultivation substrate, placing the cultivation substrate in a sunshade for cultivation, and applying a root inducing agent and a leaf fertilizer in the cultivation process to induce lateral roots of the cercis seedlings to grow until the height of the cercis seedlings is 50-60cm. Compared with the prior art, the method has the advantages that germination time is shortened by soaking seeds, lateral roots of the Chinese redbud seedlings are promoted to grow and extend by applying the culture medium, the root inducing agent and the leaf fertilizer, root weight and root system quantity are increased, and growth speed of the Chinese redbud seedlings is increased.

The invention discloses a mycorrhizal seedling culture container convenient for experimental observation. The nutrition cup is composed of a nutrition cup 1, a nutrition cup door 2, a nutrition cup door handle 3, water leakage holes 4, a black light-proof plastic box 5, a plastic plate 6 with holes, a plastic plate door 7 and a plastic plate door handle 8. The nutrition cups 1 are made of transparent plastic materials so that the growth condition of mycorrhiza can be observed at any time, water leakage holes 4 are formed in the cup bottom of each nutrition cup, an opening is formed in the cup wall, and a small door 2 capable of being opened and closed is arranged at the opening so that plant lateral roots can be conveniently taken for experimental observation. The nutrition cup 1 is placed through eight circular openings in the plastic plate 6 with holes, a rectangular opening in the middle of the plastic plate 6 is used for observing watering conditions, and a small door 7 capable of being opened and closed is arranged at the opening. The seedling culture container is placed in the black light-proof plastic box 5 in the mycorrhizal seedling culture process so as to simulate the dark environment in the soil. The novel mycorrhizal seedling culture container is suitable for observing the growth condition of mycorrhiza at any time in the mycorrhizal seedling culture process, sampling the root system of the mycorrhizal seedling at any time without damaging the whole seedling, and is beneficial to scientific research of the mycorrhizal seedling.

This disclosure relates to methods of altering crown root architecture, lateral root formation, and / or stalk height in plants by reducing or eliminating expression of a PIN-likes 6 (PILS6) gene in a plant or a plant cell. Also disclosed are plant cells, plants comprising the plant cells, nucleic acid constructs, expression vectors, and breeding methods.

The invention relates to an application of a gene GmLBMPs in regulation and control of a soybean branching development function, in particular to an application of the gene GmLBMPs in improvement of soybean yield, the gene GmLBMPs comprises a gene GmLBMPa and / or a gene GmLBMPb, and the gene GmLBMPs comprises a gene GmLBMPa and / or a gene GmLBMPb. The sequence of the GmLBMPa gene is as shown in SEQ ID NO. 1, and the sequence of the GmLBMPb gene is as shown in SEQ ID NO. 2. The invention further provides a target for knocking out the gene, a Gmlbps mutant is constructed, and the target gene of GmLBMPs can be effectively knocked out by utilizing the target provided by the invention. The research finds that compared with a wild type, the Gmlbps mutant has a longer branch, and the total pod number of a single plant is greater than that of the wild type. In addition, the experimental result also shows that the knockout of the GmLBMPs can promote the development of lateral root branches, and is more beneficial to the absorption of soybean soil moisture and nutritional ingredients. Therefore, the GmLBMPs gene has good application prospect and application value in the aspect of creating high-yield soybeans and soybeans with excellent plant types.

The technical problem to be solved by the invention is to provide the ginkgo tree seedling raising and transplanting method which can effectively promote growth of lateral roots of seedlings, facilitate lossless transplanting of the seedlings, effectively reduce the influence of difference of factors such as environmental climate of an external transplanting environment and effectively improve the transplanting survival rate. The method comprises the following steps of seedling culture room building, seed selection, seed pretreatment, planting preparation, seedling stage management, fertilization and pruning, transplanting and subsequent management. According to the seedling culture method, through seed treatment optimization, seedling culture environment control, seedling stage management and other links, the germination rate of gingko seeds and the growth speed of seedlings are effectively increased; according to the seedling raising method, an environment synchronous adaptation device is further provided, the seedlings can better adapt to the external environment after being transplanted, the transplanting survival rate is increased, the seedlings can grow more evenly, and the situation that the seedlings grow in a deviated mode due to the fact that single-side illumination is too long is avoided.

The invention relates to the technical field of soil improvement and ecological restoration, and discloses a liquorice planting method based on synergistic utilization of coal-based solid waste and desertified land, which comprises the following steps: crushing alkaline coal-based solid waste and acidic coal-based solid waste, mixing according to a mass ratio of 2: 8-4: 6, adding a modified humic acid-based heavy metal passivator and water, and uniformly mixing to obtain a mixture; uniformly mixing and aging to obtain a coal-based solid waste mixture; spreading the mixture on the surface layer of the desertification land, performing rotary tillage and deep tillage to mix the mixture with the sandy soil, and adjusting the pH value to be stable at 7.5-8.5; the treated liquorice seeds are sown, main roots are harvested in autumn of the third year, deep root systems and lateral root bud points are reserved, and overground straw is smashed and returned to the field; soil nutrition indexes are monitored regularly, other commercial crops are planted when the soil nutrition indexes reach a set threshold value, and if not, regeneration planting of the liquorice is maintained. Through synergistic compounding of acid-base solid waste and chelating of the passivating agent, substrate pH self-regulation and heavy metal resistance control are achieved, and the physicochemical property of sandy soil is improved.

This invention relates to a hydroponic root-inducing method for *Cinnamomum camphora*, characterized by the following steps: plant pretreatment; hormone-induced root treatment; perlite substrate for root induction transition; mist-controlled humidification environment for root induction; hydroponics; and disease prevention. The hydroponics includes: hydroponics in clean water until secondary lateral roots of a predetermined length are formed, followed by nutrient solution cultivation; the nutrient solution is supplied using a gradient approach. This invention's hydroponic root-inducing method employs a synergistic model of hormone-induced root induction, perlite fixation, and a high-humidity environment, significantly improving the success rate of root induction and avoiding the mortality problem caused by direct hydroponics. Through gradient acclimatization with nutrient solution concentrations, it significantly reduces the incidence of early root tip necrosis and root rot, improving survival rate and root vitality.

The invention discloses a fertilizer for promoting and improving the immunity of plants and a preparation method, and relates to the field of fertilizers. The preparation method comprises the following steps: mixing, smashing and grinding earthworms, eucalyptus leaves, wheat leaves and hawthorn leaves, putting into an ethanol solution, oscillating for 20-24 hours in a shaking table, and centrifugally filtering to obtain an active extracting solution; adding a sodium alginate solution into the suspended bacteria solution and the active extracting solution, and slowly adding carboxymethyl chitosan and a calcium chloride solution; and preparing. According to the invention, the plant growth-promoting rhizobacteria are fixed in the composite hydrogel, and the composite hydrogel can promote colonization of strains at plant root tips and lateral roots so as to regulate deposition of endothelial cell suberite, optimize mineral nutrition balance and abiotic stress response of plants and improve accumulation of auxin at the root tips; meanwhile, an active extracting solution is added into the composite hydrogel, immune response can be activated, and the metabolic activity and antioxidant activity of plants are greatly improved in combination with extracts of other substances.

The application provides a bamboo shoot seedling raising device and method, and relates to the technical field of bamboo shoot seedling raising, which comprises a supporting platform, the top end of the supporting platform is provided with a supporting frame, the top end of the supporting frame is provided with a connecting plate, and the periphery of the supporting frame is provided with a plurality of electric light curtains; a plurality of seedling raising baskets are detachably connected to the supporting platform; a plurality of lifting light supplementing mechanisms are arranged on the connecting plate, the lifting light supplementing mechanisms are connected with bamboo whip guiding mechanisms, and the bamboo whip guiding mechanisms are arranged correspondingly to the seedling raising baskets; and a spraying mechanism is arranged on the supporting platform. The seedling raising basket, low-nutrient light substrate and high-nutrient light substrate are used to avoid damage of the bamboo seedlings in the transportation process, and to improve the survival rate of transplanting. The top end advantage of the fast and robust growth of the whip tip is broken, the new bamboo whip is thickened in the high-nutrient light substrate, the number of lateral roots and the number of activated lateral buds are increased, and the quality of the nursery seedlings is ensured. The growth direction of the new bamboo whip is guided by the bamboo whip guiding mechanism, and the consistency of the nursery seedlings is improved.

The invention discloses an application of an OsCFIm68b gene in regulation and control of Cd resistance of rice and Cd accumulation of grains. The OsCFIm68b gene in rice is knocked out through gene editing to obtain a knockout mutant, and it is found that under the Cd polluted soil culture condition, the length of a main root is shortened, the number of lateral roots is reduced, the length of a total root is shortened, and the Cd concentration of grains is reduced to 1 / 3 of that of a wild type. Therefore, the OsCFIm68b gene is a negative regulatory factor for encoding Cd accumulation of the rice grains, the Cd accumulation of the rice grains can be improved by weakening the expression of the OsCFIm68b gene, the Cd resistance of the rice is improved, and a new gene target is provided for Cd low-accumulation breeding of the rice.

The invention belongs to the technical field of sugarcane cultivation, and discloses a sugarcane drought stress treatment device which comprises a bottom frame, a mounting bin is fixedly mounted on the inner wall of the bottom frame, a planting cylinder is rotatably mounted at the top of the mounting bin, the planting cylinder is divided into four sections, the length of each section is four meters, and the planting cylinder is of a high-strength aluminum alloy sleeve structural design; obstacle avoidance mechanisms are arranged on the inner walls of the planting cylinders, and stirring mechanisms are arranged on the outer walls of the planting cylinders; by arranging the obstacle avoidance mechanism and designing a threaded groove and a cylindrical groove in the inner wall of the planting barrel, definite growth guidance is provided for sugarcane roots, the roots are guided to grow downwards along the threaded groove through the design, the problem of horizontal coiling of the roots is effectively avoided, the planting barrel is driven by the motor to rotate, and the planting efficiency is improved. Teeth in the obstacle avoidance mechanism work cooperatively with a gear transmission system, effective management of growth of sugarcane minor roots is achieved, and relevant index data of sugarcane drought resistance stress in different water stress states can be accurately obtained.

The invention discloses a populus euphratica PeXTH7 gene and application of the populus euphratica PeXTH7 gene in improving drought resistance of plants, and belongs to the technical field of gene engineering. The CDS sequence of the populus euphratica PeXTH7 gene is disclosed for the first time and is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2; the effect of the populus euphratica PeXTH7 gene in improving the drought resistance of the plant is disclosed for the first time, the root length and lateral root density of the plant are remarkably improved through overexpression of the populus euphratica PeXTH7 gene, and the drought resistance of the plant is improved by adjusting the root structure of the plant and promoting root development; the populus euphratica PeXTH7 gene provided by the invention can be used as a candidate gene for breeding the drought-resistant new variety of the plant, and is of great significance for cultivating the drought-resistant new variety of the plant by adopting a genetic engineering method, accelerating the breeding process and reducing the breeding workload.

Prevent the device from being damaged by the impact of solids in the ground. 【Solution means】 It includes a drum member 61 that moves up and down while rotating in both forward and reverse directions and bites into the ground around the rootstock, and a cutter member 70 that is attached to the lower end of the drum member 61 with a gap therebetween and cuts the lateral roots of the rootstock. Block streaks 63 that bite into the ground and loosen the ground by the rotation of the drum member 61 are provided at positions close to the cutter member on the inner surface and / or outer surface of the drum member 61 in a state where they extend in the vertical direction. The cutter member 70 includes an arm member 71 that is attached to be tiltable in the reverse rotation direction of the drum member in a state of hanging down from the drum member, a cutter blade 72 that is attached to the arm member 71 so as to be located on the positive rotation direction side of the drum member, and a receiving member 73 that hangs down from the drum member so as to face the arm member on the reverse rotation direction side of the drum member and receives the tilting of the arm member.

This invention discloses a method for breeding salt-tolerant maize based on root function and artificial intelligence prediction, belonging to the field of crop breeding. It includes constructing a core germplasm resource bank, conducting high-throughput root phenotypic identification under 150mM NaCl stress, screening superior root systems based on quantitative thresholds such as a root-to-shoot ratio increase of more than 20% and lateral root density exceeding the mean by 1.5 times the standard deviation, performing dynamic multi-omics analysis at seven time points (0h, 3h, 6h) after stress to identify early response markers within 3-12h after stress, creating core parents by combining molecular markers and root traits, integrating seedling phenotypic, omics, and genotypic data, training an AI model with a prediction accuracy R² of no less than 0.65, and using the model to eliminate the last 50% of predicted potential materials in the early stages of breeding to guide parent selection. This invention achieves a paradigm shift from empirical screening to precise prediction, significantly improving breeding efficiency and directionality.