168 results about "Lateral root" patented technology

The invention relates to the technical field of plant genetic engineering, and discloses application of a soybean low-phosphorus-tolerant gene GmAP2 to improvement of low-phosphorus stress tolerance of plants. Research finds that under the condition of low phosphorus stress, the expression level of the GmAP2 gene in plants is remarkably increased. The GmAP2 gene is subjected to heterologous overexpression in arabidopsis thaliana, so that stress on plants under a low-phosphorus condition can be effectively relieved, the fresh weight and lateral root length of the arabidopsis thaliana can be remarkably increased, and the growth of the arabidopsis thaliana plants is promoted. In addition, under the low-phosphorus condition, overexpression of the GmAP2 gene can improve the phosphorus deficiency symptom of the soybean, improve the absorption of phosphorus nutrients by plants and promote the growth of plant roots and leaves. Therefore, the GmAP2 gene plays a very important role in dealing with the phosphorus starvation stress environment by the plant.

The invention discloses a growth-promoting rhizosphere bacterium streptomyces cerevisiae QS9-7 and application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of Streptomyces setonii QS9-7 is obtained through separation, and the preservation number of the Streptomyces setonii QS9-7 is CGMCC (China General Microbiological Culture Collection Center) No.32329. Experiments show that the strain QS9-7 has a relatively strong inhibition effect on various plant pathogens, and shows excellent biocontrol bacterium potential. The strain can effectively improve radicle length, germ length, lateral root number and germination rate of plant seeds and plant height and stem diameter of plant seedlings, obviously promotes plant growth, and has high application value in green prevention and control of plant diseases.

The invention provides an asiatic toddalia root twig cutting seedling raising method, and relates to the technical field of asiatic toddalia root artificial planting. The Asiatic toddalia root twig cutting seedling raising method mainly comprises the steps of selecting cutting branches, preparing a soaking solution from a 0.05% boric acid solution, IBA and DL-malic acid, treating the upper ends of the cutting branches with an ice-water bath soaking solution, treating the lower ends of the cutting branches with a normal-temperature soaking solution, soaking the cutting branches with quicklime mixed cooked rice milk and the like. According to the method, the defects in the prior art are overcome, the branch cutting rooting rate is effectively increased through treatment of the cutting branches, meanwhile, growth of lateral roots and fibrous roots is promoted, and the quality of Asiatic toddalia root cutting seedlings is improved.

The invention discloses a ZmLG3 gene and application thereof in regulation and control of nitrogen efficiency and lateral root number, and belongs to the field of plant biotechnology breeding. According to the corn ZmLG3 gene provided by the invention, after the endogenous gene generates function loss mutation in a plant or the expression of the ZmLG3 gene is inhibited, phenotypes with improved nitrogen utilization efficiency and / or increased lateral root number can be obtained, and the gene resource and the application method thereof have wide application prospects in the field of corn breeding.

The invention discloses application of a PdeTLP protein and a related biological material thereof in regulation and control of plant root system development. Experiments prove that compared with wild poplar, the number of adventitious roots, the number of adventitious root lateral roots, the root length and the root included angle of PdeTLP knockout poplar are obviously increased, and the plant height, the number of stem nodes and the stem diameter are also obviously increased. In addition, the xylem width, the phloem width and the xylem cell area of the poplar with the PdeTLP knocked out are obviously higher than those of the wild poplar. The PdeTLP gene participates in regulation and control of the root system number, the root system length, the root system included angle, the plant height, the stem node number, the stem diameter and the secondary microtubule tissue development of the poplar. The method not only has important significance on molecular mechanism and theoretical research of regulating and controlling the number and length of poplar root systems, plant height, number of stem nodes and stem diameter, but also has important guiding significance on directionally cultivating trees meeting actual production requirements.

The invention belongs to the technical field of agriculture, and particularly relates to a fertilization method for improving the quality and yield of oat seeds, diammonium hydrogen phosphate and biochar are used as base fertilizers, the application amount of the diammonium hydrogen phosphate is 10-12 kg / mu, the application amount of the biochar is 10-15 kg / mu, bacterial fertilizer dressing is carried out in the seedling emergence period of crops, and chemical fertilizer dressing is not carried out in the later period; the bacterial fertilizer is an arbuscular mycorrhizal fungus inoculant and a trichoderma inoculant. The use amount of the chemical fertilizer is reduced, and the negative effect of excessive use of the chemical fertilizer on crop growth is reduced; the soil environment is improved by additionally applying biochar, and the nutrient utilization efficiency is improved; by utilizing a hypha network formed by arbuscular mycorrhizal fungi and root systems, the absorption area of the oat root systems is increased, and the nutrient utilization efficiency of plants is improved; the application of the trichoderma can promote the development of lateral roots of plants and accelerate the growth speed of the plants in the seedling stage; the fertilization method can improve the quality and yield of the oat seeds, is simple and easy to implement, is suitable for large-scale popularization and application, and has a wide market prospect.

The invention relates to the technical field of plants, in particular to application of surfactin in promotion of rape root development. According to the invention, a new function of the surfactin is found, and the surfactin can be used as a signal molecule to promote expression of related genes of auxin of the rape and promote development of lateral roots of the rape and formation of a branch prosite, so that development of a root system of the rape is remarkably promoted. Surfactin can be used for preparing products for promoting root development of rape, such as plant growth regulators, fertilizers or fertilizer additives and the like, and has a wide application prospect.

The invention provides a root development monitoring device for pasture growth, and relates to the technical field of pasture monitoring, and the root development monitoring device comprises a planting frame, a placement plate, a cultivation assembly and an inspection mechanism; a water culture box is erected above the placement plate, the culture assembly comprises a partition plate fixedly arranged in the middle of the water culture box, four inner lining plates are fixedly arranged on the inner wall of the water culture box and located on the two sides of the partition plate, and water culture trays are placed on the upper surfaces of the four inner lining plates. According to the scheme, a transparent observation frame is finally arranged in the middle of the water culture box, and a bidirectional camera capable of adaptively ascending and descending along with the shapes of the inclined planes on the two sides of a limiting plate is arranged for observing the transparent observation frame; through the transparent observation frame, the development conditions (the shapes of main roots and lateral roots, the growth conditions of the lateral roots on the main roots, the growth degree of the main roots, whether the root systems decay or not and other conditions) of the root systems of the forage grass in the water culture box can be visually observed.

This disclosure relates to methods of altering crown root architecture, lateral root formation, and / or stalk height in plants by reducing or eliminating expression of a PIN-likes 6 (PILS6) gene in a plant or a plant cell. Also disclosed are plant cells, plants comprising the plant cells, nucleic acid constructs, expression vectors, and breeding methods.

The invention relates to the technical field of soil improvement and ecological restoration, and discloses a liquorice planting method based on synergistic utilization of coal-based solid waste and desertified land, which comprises the following steps: crushing alkaline coal-based solid waste and acidic coal-based solid waste, mixing according to a mass ratio of 2: 8-4: 6, adding a modified humic acid-based heavy metal passivator and water, and uniformly mixing to obtain a mixture; uniformly mixing and aging to obtain a coal-based solid waste mixture; spreading the mixture on the surface layer of the desertification land, performing rotary tillage and deep tillage to mix the mixture with the sandy soil, and adjusting the pH value to be stable at 7.5-8.5; the treated liquorice seeds are sown, main roots are harvested in autumn of the third year, deep root systems and lateral root bud points are reserved, and overground straw is smashed and returned to the field; soil nutrition indexes are monitored regularly, other commercial crops are planted when the soil nutrition indexes reach a set threshold value, and if not, regeneration planting of the liquorice is maintained. Through synergistic compounding of acid-base solid waste and chelating of the passivating agent, substrate pH self-regulation and heavy metal resistance control are achieved, and the physicochemical property of sandy soil is improved.

This invention relates to a hydroponic root-inducing method for *Cinnamomum camphora*, characterized by the following steps: plant pretreatment; hormone-induced root treatment; perlite substrate for root induction transition; mist-controlled humidification environment for root induction; hydroponics; and disease prevention. The hydroponics includes: hydroponics in clean water until secondary lateral roots of a predetermined length are formed, followed by nutrient solution cultivation; the nutrient solution is supplied using a gradient approach. This invention's hydroponic root-inducing method employs a synergistic model of hormone-induced root induction, perlite fixation, and a high-humidity environment, significantly improving the success rate of root induction and avoiding the mortality problem caused by direct hydroponics. Through gradient acclimatization with nutrient solution concentrations, it significantly reduces the incidence of early root tip necrosis and root rot, improving survival rate and root vitality.

The invention discloses a fertilizer for promoting and improving the immunity of plants and a preparation method, and relates to the field of fertilizers. The preparation method comprises the following steps: mixing, smashing and grinding earthworms, eucalyptus leaves, wheat leaves and hawthorn leaves, putting into an ethanol solution, oscillating for 20-24 hours in a shaking table, and centrifugally filtering to obtain an active extracting solution; adding a sodium alginate solution into the suspended bacteria solution and the active extracting solution, and slowly adding carboxymethyl chitosan and a calcium chloride solution; and preparing. According to the invention, the plant growth-promoting rhizobacteria are fixed in the composite hydrogel, and the composite hydrogel can promote colonization of strains at plant root tips and lateral roots so as to regulate deposition of endothelial cell suberite, optimize mineral nutrition balance and abiotic stress response of plants and improve accumulation of auxin at the root tips; meanwhile, an active extracting solution is added into the composite hydrogel, immune response can be activated, and the metabolic activity and antioxidant activity of plants are greatly improved in combination with extracts of other substances.

The application provides a bamboo shoot seedling raising device and method, and relates to the technical field of bamboo shoot seedling raising, which comprises a supporting platform, the top end of the supporting platform is provided with a supporting frame, the top end of the supporting frame is provided with a connecting plate, and the periphery of the supporting frame is provided with a plurality of electric light curtains; a plurality of seedling raising baskets are detachably connected to the supporting platform; a plurality of lifting light supplementing mechanisms are arranged on the connecting plate, the lifting light supplementing mechanisms are connected with bamboo whip guiding mechanisms, and the bamboo whip guiding mechanisms are arranged correspondingly to the seedling raising baskets; and a spraying mechanism is arranged on the supporting platform. The seedling raising basket, low-nutrient light substrate and high-nutrient light substrate are used to avoid damage of the bamboo seedlings in the transportation process, and to improve the survival rate of transplanting. The top end advantage of the fast and robust growth of the whip tip is broken, the new bamboo whip is thickened in the high-nutrient light substrate, the number of lateral roots and the number of activated lateral buds are increased, and the quality of the nursery seedlings is ensured. The growth direction of the new bamboo whip is guided by the bamboo whip guiding mechanism, and the consistency of the nursery seedlings is improved.

The invention discloses an application of an OsCFIm68b gene in regulation and control of Cd resistance of rice and Cd accumulation of grains. The OsCFIm68b gene in rice is knocked out through gene editing to obtain a knockout mutant, and it is found that under the Cd polluted soil culture condition, the length of a main root is shortened, the number of lateral roots is reduced, the length of a total root is shortened, and the Cd concentration of grains is reduced to 1 / 3 of that of a wild type. Therefore, the OsCFIm68b gene is a negative regulatory factor for encoding Cd accumulation of the rice grains, the Cd accumulation of the rice grains can be improved by weakening the expression of the OsCFIm68b gene, the Cd resistance of the rice is improved, and a new gene target is provided for Cd low-accumulation breeding of the rice.

The invention belongs to the technical field of sugarcane cultivation, and discloses a sugarcane drought stress treatment device which comprises a bottom frame, a mounting bin is fixedly mounted on the inner wall of the bottom frame, a planting cylinder is rotatably mounted at the top of the mounting bin, the planting cylinder is divided into four sections, the length of each section is four meters, and the planting cylinder is of a high-strength aluminum alloy sleeve structural design; obstacle avoidance mechanisms are arranged on the inner walls of the planting cylinders, and stirring mechanisms are arranged on the outer walls of the planting cylinders; by arranging the obstacle avoidance mechanism and designing a threaded groove and a cylindrical groove in the inner wall of the planting barrel, definite growth guidance is provided for sugarcane roots, the roots are guided to grow downwards along the threaded groove through the design, the problem of horizontal coiling of the roots is effectively avoided, the planting barrel is driven by the motor to rotate, and the planting efficiency is improved. Teeth in the obstacle avoidance mechanism work cooperatively with a gear transmission system, effective management of growth of sugarcane minor roots is achieved, and relevant index data of sugarcane drought resistance stress in different water stress states can be accurately obtained.

The invention discloses a populus euphratica PeXTH7 gene and application of the populus euphratica PeXTH7 gene in improving drought resistance of plants, and belongs to the technical field of gene engineering. The CDS sequence of the populus euphratica PeXTH7 gene is disclosed for the first time and is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2; the effect of the populus euphratica PeXTH7 gene in improving the drought resistance of the plant is disclosed for the first time, the root length and lateral root density of the plant are remarkably improved through overexpression of the populus euphratica PeXTH7 gene, and the drought resistance of the plant is improved by adjusting the root structure of the plant and promoting root development; the populus euphratica PeXTH7 gene provided by the invention can be used as a candidate gene for breeding the drought-resistant new variety of the plant, and is of great significance for cultivating the drought-resistant new variety of the plant by adopting a genetic engineering method, accelerating the breeding process and reducing the breeding workload.

Prevent the device from being damaged by the impact of solids in the ground. 【Solution means】 It includes a drum member 61 that moves up and down while rotating in both forward and reverse directions and bites into the ground around the rootstock, and a cutter member 70 that is attached to the lower end of the drum member 61 with a gap therebetween and cuts the lateral roots of the rootstock. Block streaks 63 that bite into the ground and loosen the ground by the rotation of the drum member 61 are provided at positions close to the cutter member on the inner surface and / or outer surface of the drum member 61 in a state where they extend in the vertical direction. The cutter member 70 includes an arm member 71 that is attached to be tiltable in the reverse rotation direction of the drum member in a state of hanging down from the drum member, a cutter blade 72 that is attached to the arm member 71 so as to be located on the positive rotation direction side of the drum member, and a receiving member 73 that hangs down from the drum member so as to face the arm member on the reverse rotation direction side of the drum member and receives the tilting of the arm member.

This invention discloses a method for breeding salt-tolerant maize based on root function and artificial intelligence prediction, belonging to the field of crop breeding. It includes constructing a core germplasm resource bank, conducting high-throughput root phenotypic identification under 150mM NaCl stress, screening superior root systems based on quantitative thresholds such as a root-to-shoot ratio increase of more than 20% and lateral root density exceeding the mean by 1.5 times the standard deviation, performing dynamic multi-omics analysis at seven time points (0h, 3h, 6h) after stress to identify early response markers within 3-12h after stress, creating core parents by combining molecular markers and root traits, integrating seedling phenotypic, omics, and genotypic data, training an AI model with a prediction accuracy R² of no less than 0.65, and using the model to eliminate the last 50% of predicted potential materials in the early stages of breeding to guide parent selection. This invention achieves a paradigm shift from empirical screening to precise prediction, significantly improving breeding efficiency and directionality.

This utility model relates to the field of soybean planting technology, specifically disclosing a soybean planting box, including a base, a water collection tank fixedly connected to the upper end of the base, multiple planting box bodies arranged above the water collection tank, an adjustment mechanism arranged between the multiple planting box bodies, multiple evenly distributed conical protrusions arranged at the bottom of the inner wall of each of the multiple planting box bodies, and multiple drainage holes arranged alternately with the conical protrusions through the lower end of each of the multiple planting box bodies, and planting substrate filled in each of the multiple planting box bodies. By setting the conical protrusions at the bottom of the inner wall of the planting box body to form a three-dimensional slope, the main root of soybean is guided to grow down along the sloping surface of the protrusion, while the lateral roots extend laterally from the gaps between the cones, avoiding the root entanglement caused by the traditional flat bottom wall. The conical structure makes the bottom wall surface form a non-planar drainage channel, and excess water flows quickly to the surroundings along the sloping surface of the cone and is discharged from the drainage holes, preventing soybean roots from rotting due to waterlogging.

This invention discloses the application of the CLE11 small peptide gene in regulating plant salt tolerance, belonging to the field of biomaterials technology. This invention uses millet as the research material and clones... SiPROCLE11 Gene (CLE11 small peptide gene), construction SiPROCLE11 Overexpression and silencing of millet materials, exogenous application of the synthetic peptide SiCLE11p, and its role in enhancing millet salt tolerance were determined. Further investigation revealed that it can promote lateral root development and reduce Na+ in the roots. + This accumulation of knowledge laid the foundation for the discovery of salt-tolerant genes in millet and for salt-tolerant millet breeding.

The invention discloses application of a compound 5-HIAA in improving the transcription or expression level of a plant root auxin related gene, and belongs to the technical field of plant hormone auxin. The application of 5-hydroxyindole-3-acetic acid (5-HIAA) in improving the transcription or expression level of plant root auxin related genes is found for the first time, growth of lateral roots, root hair and adventitious roots of plants can be effectively promoted in a non-toxic range, the 5-hydroxyindole-3-acetic acid has broad-spectrum auxin activity, and the application of 5-HIAA in improving the transcription or expression level of the plant root auxin related genes is promoted. The auxin analogue disclosed by the invention can be used as an animal-derived auxin analogue to regulate and control the growth and development of a plant root system, and can be perceived by a plant auxin co-receptor TIR1 / AFBs-Aux / IAAs, so that plant rooting is promoted, and animal-plant information exchange is mediated.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a peach tree MADS BOX transcription factor PpAGL24 gene and application thereof. Specifically, an MADS BOX transcription factor PpAGL24 gene is cloned in a peach tree root system, and the nucleotide sequence of the gene is shown as SEQ ID NO.1. Molecular biology experiments and genetic engineering means prove that the gene actively responds to low-nitrogen signals, peach lateral root generation and nitrogen absorption and utilization can be promoted, the nitrogen utilization efficiency of transgenic plants can be effectively improved, and the transgenic plants can be applied to the field of plant growth. Gene resources are provided for peach tree breeding with high nitrogen utilization efficiency, the current situation that nitrogen fertilizer is excessively used due to low nitrogen utilization efficiency in peach tree production can be solved, and the application prospect is very high.

The invention discloses a rapid transplanting device and method suitable for angelica sinensis, and belongs to the technical field of plant transplanting. Comprising a vehicle frame, the vehicle frame is rotationally connected with a second rotating shaft arranged in a mirror image mode, the second rotating shaft is fixedly connected with chain wheels arranged in a mirror image mode, a chain is wound between the chain wheels located on the same vertical face, the chain is fixedly connected with fixing pieces arranged at intervals, and the fixing pieces are in spline connection with first limiting pieces; the first limiting piece is fixedly connected with an air bag through a first moving piece, the frame is provided with a first limiting groove, and the first limiting groove is used for limiting the first limiting piece. The first limiting parts are limited by the first limiting grooves, so that the adjacent first limiting parts move oppositely, and angelica sinensis is vertically clamped in the transplanting process by utilizing the form change of the air bags, so that the lateral root cluster degree of the angelica sinensis in the subsequent growth process is reduced, and the subsequent growth quality of the angelica sinensis is improved.

The invention belongs to the technical field of plant tissue culture, and relates to a tissue culture and rapid propagation method for castanopsis sclerophylla. According to the method, castanopsis sclerophylla seeds are used as materials, and through sterilization method comparison, basic culture medium screening, hormone proportioning optimization and the like, an efficient tissue culture and rapid propagation technical system of castanopsis sclerophylla is established through seed sterile germination, bud proliferation, strong bud rooting and acclimatization and transplantation, and complete tissue culture progeny plants are formed. 100.0% of the seeds in the system germinate and sprout; the propagation coefficient of buds is large and reaches 6.8; the rooting rate reaches 92.8%, the root system is developed, the average is 4.1 roots per plant, the root length is 4.6 cm, and lateral roots are dense; the transplanting survival rate is high and can reach 95.2% or above; and the method is not limited by factors such as seasons, climate and regions, and can realize annual efficient and rapid propagation. According to the method, the blank of tissue culture and rapid propagation of the castanopsis sclerophylla is filled, a stable and efficient regeneration method for the in-vitro culture plant of the castanopsis sclerophylla is provided, large-scale seedling culture can be carried out, the cultured castanopsis sclerophylla seedlings are robust, neat and consistent, and a high-quality seedling guarantee is provided for improving the economic benefit of an artificial forest of the castanopsis sclerophylla.

The present invention discloses a strain of Enterobacter huaxiensis 314A1 and its application. The Enterobacter huaxiensis 314A1 was deposited at the China Center for Type Culture Collection on October 31, 2023, with the deposit number: CCTCC NO: M20232090. In the present invention, the bacterial powder is used as a seed dressing agent to dress wheat seeds and then sow them. This bacterial powder can accelerate the germination and early growth rate of wheat, and at the same time can increase the biomass of wheat, specifically manifested as promoting the development of wheat lateral roots, increasing the biomass of wheat, accelerating the germination rate of wheat seeds, and increasing the yield of wheat.

The application discloses a kind of quick transplanting equipment and method suitable for angelica sinensis belonging to plant transplanting technical field.It includes: frame, the frame rotationally connected with mirror image arranged second rotating shaft, the second rotating shaft is fixedly connected with mirror image arranged sprocket, chain is wound between the sprocket in the same vertical plane, the chain is fixedly connected with interval arranged fixing part, the fixing part spline connection has first limiting part, the first limiting part is fixedly connected with air bag by first moving part, the frame is provided with first limiting slot, and the first limiting slot is used to limit the first limiting part.The limiting of first limiting slot to first limiting part makes adjacent first limiting part move oppositely, and the vertical clamping of angelica sinensis during transplanting is carried out using the morphological change of air bag, so as to reduce the degree of lateral root proliferation of angelica sinensis in subsequent growth process, and then improve the growth quality of angelica sinensis subsequently.

The invention provides a gene for regulating nitrogen absorption and root growth under salt stress and application of the gene. OsGSK1 gene is knocked out from rice to obtain OsGSK1 mutant rice; the nucleotide sequence of the OsGSK1 gene is as shown in SEQ ID NO: 1. According to the invention, the OsGSK1 gene in rice is knocked out to generate frame-shift mutation, and the OsGSK1 mutant rice is obtained. A mutant strain is compared with a wild strain, the lateral root density of the mutant in a high-salt environment is remarkably higher than Taizhong 65, and meanwhile, the total nitrogen content and the nitrogen absorption and utilization rate of the overground part of the mutant strain are also remarkably higher than Taizhong 65. The results show that the rice OsGSK1 gene has potential application value in improvement of nitrogen utilization under rice salt stress, and has important significance in stress-resistant breeding of rice and improvement of rice yield.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of an LsSLR1 gene or a related biological material containing the LsSLR1 gene in plant character improvement. The invention discloses a nucleotide sequence of an LsSLR1 gene as shown in SEQ ID NO.3. A recombinant expression vector pCAMBIA1300-GFP-LsSLR1 is constructed, the recombinant expression vector is introduced into a model plant arabidopsis thaliana, and a transgenic arabidopsis thaliana plant over-expressing the LsSLR1 is obtained. Experiments find that overexpression of the LsSLR1 gene can promote development of lateral roots of plants, dwarf the plants, shorten the growth cycle of the plants and improve stress resistance of the plants to salt stress, drought stress or abscisic acid stress, and genetic improvement can be performed on plant traits by using the LsSLR1 gene.

The invention discloses a slope surface protection supporting structure of a high and steep soil slope and a construction method of the slope surface protection supporting structure, and relates to the field of slope supporting. S2, a traditional slope supporting structure is constructed, and the stability is detected; s3, digging a planting pit and carrying out wall protection treatment; s4, a root system control cage body is installed and fixed; s5, seedlings are planted and cultivated in the root system control cage body, and lateral roots extend into the soft soil body area in a directional mode; s6, the plants and the root system control cage are removed, and root system holes are protected; s7, a grouting anchoring assembly is arranged; s8, performing pressure grouting construction; s9, maintaining the grouting body and detecting the stability of the side slope; and S10, the planting pits are sealed and filled, and slope ending is completed. The problems that according to a traditional grouting technology, grout is difficult to permeate into a soft area in a soil body, and gaps are prone to being generated on the combination face of the grout and the soil body are solved, the grouting body can deeply permeate and be tightly combined with the soil body, and the overall construction technology gives consideration to the short-term ecological soil fixation requirement and the long-term engineering stability requirement.

The invention discloses a plant root system lossless seedling raising supporting device and a seedling raising method, and belongs to the technical field of plant seedling raising and root system research. The invention aims to solve the problems of root hair tearing and lateral root damage during seedling transfer or root system observation due to dependence on a solid substrate in the existing seedling raising technology. The device comprises a supporting base of an annular opening and closing structure, first supporting columns and second supporting columns which are alternately and obliquely arranged are arranged on the lower side of the supporting base and form a necking structure, supporting filter paper is arranged in the necking structure, and drainage filter paper making contact with the supporting filter paper is detachably connected to the supporting base; the seedling raising method is completed through device assembly, nutrient solution supply, seed cultivation, lossless seedling taking and device reuse. Seeds are supported in an auxiliary mode through a small amount of ceramsite on the supporting filter paper, nutrients are conveyed through the drainage filter paper, seedlings are taken out in a lossless mode in cooperation with the open-close type supporting base, it is guaranteed that the root system structure is complete, and the device is simple in structure, convenient to assemble, reusable and suitable for large-scale root system research and crop breeding and seedling raising operation.