Rice lateral root control gene osiaa11 and its application

A gene and lateral root technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2011-12-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies in monocotyledonous crops are rarely reported

Method used

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  • Rice lateral root control gene osiaa11 and its application
  • Rice lateral root control gene osiaa11 and its application
  • Rice lateral root control gene osiaa11 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1, Screening and phenotype of mutants

[0042] Using the Kasalath mutant library induced by EMS as the mutant screening object, M 2 Substitute seeds were rinsed with distilled water and diluted with 0.6% (v / v) HNO 3 Dormancy-breaking treatment for 16 hours, germination in the dark at 37°C until white. Sow the lubai seeds on the nylon mesh floating on the rice culture solution (the formula of the culture solution is the standard formula of the International Rice Institute), and cultivate it for 7 hours at a temperature of about 30 / 22°C (day / night) and 12 hours of light. Day, with the change of lateral roots (including length, quantity, etc.) as screening criteria for the screening of mutants, from which a mutant without lateral roots was screened ( figure 1 A-F), ie, rice lateral root development-deficient mutant Osiaa11.

Embodiment 2

[0043] Embodiment 2, gene localization

[0044] f 2 The mapping population was obtained by crossing the homozygous (Osiaa11) as the female parent and the wild-type japonica cultivar Nipponbare as the male parent, and a total of 186 F 2 Individuals, using the rapid extraction method of rice trace DNA to extract genomic DNA for gene mapping from rice leaves. Take about 2cm young rice leaves, freeze them with liquid nitrogen, grind the leaves into powder in a 1.5ml centrifuge tube, extract the total DNA, and dissolve the obtained DNA in 200 μl sterile water. 2 μl of DNA sample was used for each SSR and STS reaction.

[0045] 1), in the preliminary localization test of OsIAA11 gene, for 30 F 2 Individuals were subjected to SSR analysis. According to the published molecular genetic maps created by japonica and indica rice, select SSR primers that are approximately evenly distributed on each chromosome, perform PCR amplification according to known reaction conditions, and then s...

Embodiment 3

[0059] Example 3, gene prediction and sequence analysis

[0060] According to the results of fine mapping, the OsIAA11 gene is located within the range of RM6266 and RM5626 on the BAC clone OSJNBb0069P02 ( figure 2 A). According to the rice gene annotation information of TIGR (http: / / www.tigr.org / tdb / e2k1 / osa1 / ), the gene prediction analysis of the mapped chromosome segment shows that there are 2 AUX / IAA genes in this segment. Using Kasalath wild-type and Osiaa11 mutant cDNA as templates to amplify the two candidate genes, the amplified products were sequenced separately, and the sequencing results were compared and analyzed. It was found that the base C at the 317th bp after the start codon ATG of the IAA11 gene was Mutated into a T, resulting in an amino acid change from proline to leucine, this point mutation has been verified by dCAPs ( figure 2 B).

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Abstract

The invention discloses a protein coded by a rice lateral root control gene OsIAAII, which has an amino acid sequence represented by SEQ ID No.2. The invention also discloses the gene coding the protein, which has a nucleotide sequence shown by SEQ ID No.1. The gene of the invention can be used for building transgenic rice having a rice lateral root control function. As the normal germination andgrowth of lateral roots are critical to the maintenance of the growth and high and stable yield of rice and the gene only affects the germination and growth of the lateral roots, the application potential of the gene in molecular breeding is high.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering. Specifically, the present invention relates to a genome sequence of the rice OsIAA11 gene cloned by a map-based cloning technique. Function gain transgenic experiments and screening of self-suppressing mutants prove that the gene controls the development of lateral roots. Background technique [0002] Uptake of water and nutrients from the soil and anchoring are two fundamental functions of plant roots. Correct root morphogenesis is essential to perform its two basic functions, and rice, as the second largest model plant for root system research, has more important agricultural production significance. There are 2.5 billion people in the world who use rice as their main food, and about 9% of the world's available arable land is used to grow rice. Rice accounts for 21 percent of energy expenditure and 15 percent of protein intake per capita. Rice belongs to the monocotyledonous gramineo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N5/10A01H5/00
Inventor 吴平朱振兴毛传澡吴运荣
Owner ZHEJIANG UNIV
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