2099 results about "Molecular breeding" patented technology

The invention discloses a litopenaeus vannamei body length growth trait associated SNP molecular marker, a detection primer and application thereof. The sequence of the SNP molecular marker is as shown in SEQ ID NO.1, the SNP site is located at the 282 site from the 5'end of the sequence as shown in SEQ ID NO.1, and the polymorphism of the SNP molecular marker is T / C type. The SNP locus can be used for body length screening of litopenaeus vannamei in litopenaeus vannamei assisted breeding, and a primer designed according to the sequence of SEQ ID NO.1 or a kit prepared from the primer can be used for detecting the SNP locus. The SNP molecular marker Chr3711743678 provided by the invention can be used for assisting in selecting rapid growth parents, and the CC genotype in the SNP molecular marker is used for selecting the parents, so that the growth speed of breeding populations can be increased, an innovative tool is provided for molecular breeding of litopenaeus vannamei, the breeding period is shortened, and industrial development and efficiency improvement are assisted.

The invention discloses an interpretable machine learning genome prediction method and device, belongs to the technical field of combination of biological breeding, biological information and machine learning, and utilizes an advanced machine learning algorithm to perform parameter optimization in combination with biological prior information. Through processing of multi-source data (genome, transcriptome and epigenetic data), dynamic feature engineering (PCA and PHATE dimensionality reduction) and organic combination of various machine learning models, and an automatic parameter adjustment framework based on a grid search and sparrow search algorithm, genome prediction precision and calculation efficiency are significantly improved; meanwhile, the interpretability of the model is realized based on the SHAP value, the SNP site contribution is quantified, a reference is provided for precise breeding, and the method is suitable for animal and plant molecular breeding and medical genetic analysis, and can accelerate genetic analysis of high-value characters, assist precise breeding decision and disease risk prediction, and promote leap-forward development from experience breeding to intelligent breeding.

The invention belongs to the technical field of molecular biotechnology and molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker located on a chromosome 2 and related to abnormal delivery traits of sows and application of the SNP molecular marker. An SNP site of the SNP molecular marker corresponds to Cgt at the 23330829bp position on a chromosome 2 of an international pig reference genome version 11.1; and the polymorphism of the basic group at the site affects the abnormal delivery character of the sow. The method solves the problem of genetic improvement of abnormal delivery of sows by means of molecular breeding, and can increase the frequency of dominant alleles generation by generation, reduce the occurrence rate of abnormal delivery of sows and improve the reproductive performance of sows by optimizing the dominant alleles of variation sites. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention belongs to the technical field of crop genetic breeding, and particularly relates to a rice hybrid male sterility site HMS10 co-segregation molecular marker and application thereof. The method comprises the following steps: hybridizing and backcrossing an anti-thermo-sensitive type genic male sterile line Yannong S serving as a female parent with a normal fertile material Yannong HB (serving as a recurrent male parent), finely positioning to a site HMS10 for controlling hybrid male sterility of rice in a physical interval of-60kb of a chromosome 10 of the rice by utilizing a high-generation backcross large population, and developing a co-separation molecular marker IDHMS10. The molecular marker IDHMS10 is utilized, through a molecular breeding strategy, a positive temperature-sensitive male genic male sterile line Quan 211S with excellent comprehensive agronomic traits is transformed into a practical rice hybrid male sterile line Quan 211HS with fertility not affected by light and temperature, and the IDHMS10 is used for purity identification of the hybrid male sterile line, so that the molecular marker IDHMS10 has great significance in solving the seed production risk of two-line hybrid rice and improving the yield of the hybrid male sterile line. And the method has important significance in enriching practical sterile resource types and the like.

The invention provides an SNP molecular marker combination for pop corn whole genome typing, a detection chip and application, and relates to the technical field of molecular breeding. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination for genotyping of the whole genome of the pop corn, a genotyping object comprises 6419 SNP loci, the SNP markers uniformly cover the whole corn reference genome, and a high-quality molecular marker can be provided for map-based cloning of a pop corn specific agronomic trait control gene. Meanwhile, different types of corn can also be subjected to genotyping. Burst corn germplasm resources are narrower than other types of corn germplasm resources, and the chip can overcome the defect that an existing chip developed based on grain corn diversity is low in diversity in pop corn genotyping.

The invention relates to the technical field of bioinformatics, and provides a visual analysis method and system for a rice multi-tissue single cell expression profile. The method comprises the following steps: comparing sequencing data of an original single cell transcriptome of a rice tissue to obtain a standardized transcriptome data set; performing batch effect correction and integration on the standardized transcriptome data set to obtain a whole plant expression matrix; performing cell type annotation on the whole plant expression matrix to obtain a cell type annotation system; carrying out visual dimension reduction processing on the whole plant expression matrix fused with the cell type annotation system, and carrying out co-expression network construction to obtain a modular tissue correlation analysis model; and establishing an interaction end based on the module organization correlation analysis model, and realizing data visualization analysis through the interaction end. The invention provides a one-stop analysis platform for rice cell heterogeneity research, functional gene mining and molecular breeding.

The invention discloses an SNP molecular marker related to the meat percentage character of procambarus clarkia and application of the SNP molecular marker, genetic typing is performed on the procambarus clarkia through whole genome association analysis, whole genome association analysis is performed on the meat percentage of the procambarus clarkia, and the SNP marker remarkably related to the meat percentage character is screened out and comprises one or more of SNP1-SNP10. By detecting the SNP marker, the procambarus clarkii with high growth speed and high meat percentage can be effectively predicted and bred, the breeding efficiency and accuracy of the procambarus clarkii are remarkably improved, a new variety of the procambarus clarkii with high yield and high quality is bred, and the SNP marker has a wide application prospect in the aspect of molecular breeding of the procambarus clarkii with the meat percentage character.

The invention belongs to the technical field of whole-genome gene chips, and particularly relates to a leymus chinensis whole-genome 50K liquid chip and application thereof. According to the leymus chinensis whole genome 50K liquid phase chip, a whole genome re-sequencing technology is utilized, an SNP molecular marker combination of leymus chinensis is determined, and then the leymus chinensis whole genome 50K liquid phase chip is developed. The chip is combined with a high-density SNP probe, realizes high-throughput and high-precision genetic typing of leymus chinensis genomes, and particularly has wide application prospects in the field of molecular breeding.

The invention discloses an OsFBX421 gene and application of the OsFBX421 gene in the aspect of improving the salt tolerance of rice. The nucleotide sequence of the OsFBX421 gene is as shown in SEQ ID NO: 1 in a sequence table. The invention further discloses a phenotype of an OsFBX421 gene mutant strain under treatment of 120 mM NaCl, and the protein and the coding gene thereof have important theoretical and practical significance on research of a plant salt-tolerant mechanism and improvement of salt tolerance and related traits of plants, can play an important role in plant salt-tolerant molecular breeding and have wide application prospects.

The invention discloses a KASP molecular marker primer combination for detecting peanut late spot disease resistance and application of the KASP molecular marker primer combination, and belongs to the technical field of peanut molecular breeding. The molecular marker linked with the late spot resistance character can be used for detecting the genotype of peanuts so as to judge whether a material has late spot resistance or not, through high-throughput genotyping detection, manpower and material resources are greatly saved, the detection is not influenced by environmental factors, the result is accurate and reliable, and the molecular marker can be used for early screening of peanut late spot resistance varieties and can be applied to early screening of peanut late spot resistance varieties. The breeding period can be shortened and the breeding efficiency is improved.

The invention is applicable to the technical field of bioengineering, and provides a coding gene for enhancing iron deficiency stress tolerance of plants and application. According to the soybean GmMYB14 transcription factor disclosed by the invention, the problem that the growth of soybeans is limited in an iron-deficient environment, particularly calcareous soil and soda saline alkali soil, is solved by excavating and applying the encoding gene (the nucleotide sequence is shown as SEQ ID No.1) of the soybean GmMYB14 transcription factor. Experiments prove that after the gene is over-expressed in arabidopsis thaliana and soybean, the tolerance of plants to iron deficiency stress can be remarkably enhanced. The invention provides a key gene resource for molecular breeding: the GmMYB14 effectively promotes the absorption and utilization of plants on iron by regulating and controlling the metabolic pathway of phenylpropane, not only can improve the planting adaptability of soybeans in low-iron soil, but also can improve the yield and quality of the soybeans, provides powerful technical support for enlarging the planting area of the soybeans in iron-deficient soil regions, and has a wide application prospect. The method is of great significance in relieving insufficient soybean productivity in China and improving the self-sufficiency rate.

The invention discloses a mulberry SNP (Single Nucleotide Polymorphism) marker mining and stress resistance character prediction method based on a Transform architecture, and belongs to the crossing field of biotechnology and artificial intelligence. According to the method, mulberry genome sequencing and stress resistance phenotype data are collected, high-credibility SNP is screened through quality filtering and Bayesian statistics, a Transform model containing multiple self-attention mechanisms is constructed after multi-dimensional features are extracted, early stop method training optimization is combined, stress resistance character prediction is achieved, and the key SNP contribution degree is analyzed through feature disturbance. According to the method, the problems of insufficient long-distance feature correlation capture, low prediction precision, poor model interpretability and the like in the traditional technology are effectively solved, the stress resistance character prediction precision and the SNP marker credibility are improved, and an interpretable efficient screening strategy is provided for mulberry molecular breeding.

The invention discloses a gene PtoMYB20 for regulating and controlling salt tolerance and drought resistance of poplars. A nucleotide sequence of the gene comprises a sequence as shown in SEQ ID NO. 1. The gene interference fragment is introduced into a plant to obtain a silent transgenic plant, under the conditions of salt stress, drought stress and salt-drought composite stress, the H2O2 and MDA contents of the silent transgenic plant are lower than those of a wild plant, and the SOD and POD activities of the silent transgenic plant are higher than those of the wild plant, so that the damage degree of the plant is effectively reduced; the method is of great significance in improving salt tolerance and drought resistance of the poplar and relieving damage of adversity stress to forestry yield. The invention also discloses a breeding method of the transgenic poplar with strong salt tolerance and drought resistance, provides a new poplar germplasm which not only improves the stress resistance of the poplar but also does not influence the wood yield, and lays a theoretical and gene resource foundation for the stress-resistant molecular breeding of the poplar.

The invention belongs to the technical field of molecular genetic breeding, and particularly relates to a molecular marker Zm00001eb181850 gene related to corn leukoderma resistance and application thereof, the invention provides a molecular marker related to the corn leukoderma resistance, the gene sequence of the molecular marker is shown as SEQ ID NO: 1 and / or SEQ ID NO: 2, and the molecular marker Zm00001eb181850 gene is shown as SEQ ID NO: 1 and / or SEQ ID NO: 2. Researches prove that corn white spot resistance QTL qMWS4-1 and qMWS4-5 intervals and resistance SNP: 4-118983592 have co-localization, the corn white spot resistance QTL has a high phenotypic interpretation rate, the associated gene Zm00001eb181850 (SEQ ID NO: 2) is highly expressed in corn leaves, it is proved that the associated gene Zm00001eb181850 (SEQ ID NO: 2) is associated with the corn leaf spot, the corn white spot resistance is a quantitative character jointly regulated by multiple genes, and the corn leaf spot resistance QTL has a good application prospect. According to the technical scheme provided by the invention, a new method is provided for cultivating a new variety of leukoderma-resistant corn by utilizing a modern molecular breeding technology.

The invention discloses a molecular marker closely linked with wheat flag leaf width major QTL and application of the molecular marker in prediction of wheat flag leaf width, and belongs to the technical field of wheat molecular biotechnology and breeding. Wherein the molecular marker is 4BFLW-290, the nucleotide sequences of the molecular marker are as shown in SEQ ID NO: 3 and SEQ ID NO: 4, the specific primer pair of the molecular marker is 4BFLW-290-F / R, and the nucleotide sequences of the specific primer pair are as shown in SEQ ID NO: 1 and SEQ ID NO: 2. The molecular marker 4BFLW-290 provided by the invention fully embodies the flag leaf width major QTL of the wheat variety (line), can quickly and accurately predict whether the wheat variety (line) has the flag leaf width major QTL or not, and provides excellent gene resources and selection tools for wheat yield trait molecular breeding.

Provided is the use of overexpression of the auxin transporter PtoPIN1a gene in increasing the biomass of Populus tomentosa. The nucleotide sequence of the auxin transporter PtoPIN1a gene is as shown in SEQ ID No. 1. By means of using a genetic engineering method, wild-type Populus tomentosa is transformed by means of Agrobacterium tumefaciens infection to obtain a tissue-specific PtoPIN1a overexpressing transgenic plant, and subsequently, a plant with a relatively high expression level is selected, by means of positive identification, for phenotype observation. Results show that compared with the wild type in the same period, the PtoPIN1a transgenic plant has an increased plant height, a thicker stem and a significantly increased aboveground biomass. By means of the provided technical cultivation method, a high-biomass high-quality poplar variety is cultivated. The method is of great significance for future molecular breeding to cultivate excellent poplar varieties.

The invention discloses application of a PagBAG8 gene in regulation and control of poplar growth and development, and belongs to the technical field of forestry biological genetic engineering. According to the application of the PagBAG8 gene in regulating and controlling growth and development of the poplar, the nucleotide sequence of the PagBAG8 gene is shown as SEQ ID NO: 1, and the method for cultivating the transgenic poplar through the PagBAG8 gene comprises the following steps that the PagBAG8 gene of the poplar is cloned; the PagBAG8 gene is connected to a vector, and an overexpression vector is obtained; transforming the obtained overexpression vector into agrobacterium to obtain an agrobacterium liquid; and infecting poplar leaves with the obtained agrobacterium liquid to obtain a PagBAG8 gene overexpression plant. The invention provides a new choice for regulating and controlling the growth and development of the poplar, and provides a potential target for molecular breeding improvement of the properties of the poplar.

The invention belongs to the technical field of molecular biotechnology and molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) site related to pig intramuscular fat content, a related molecular marker and application of the SNP site. The SNP site corresponds to the 151330919bp site of a No.6 chromosome of an international pig reference genome version 11.1, the polymorphism of a base of the site affects the intramuscular fat content of the pig, the related index of a TT genotype is greater than that of a pig individual with TG and GG genotypes, and the related index of the TG genotype is greater than that of a pig individual with GG genotype. According to the invention, a molecular breeding means is utilized to solve the problem of improving the character expression of the intramuscular fat content of the pig, and by optimizing the dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the intramuscular fat content of the pig can be improved, the pork quality can be improved, the genetic modification breeding of the pig can be accelerated, and the economic benefit of a pig farm can be improved.

The invention belongs to the technical field of gene engineering, and relates to a cyperus esculentus CeMYB154 gene capable of improving salt tolerance and active oxygen scavenging capacity and application of the cyperus esculentus CeMYB154 gene. The Cyperus esculentus CeMYB154 gene belongs to an MYB transcription factor family, the Cyperus esculentus CeMYB154 gene is screened and transformed into Arabidopsis thaliana through transcriptome sequencing, bioinformatics analysis, gene cloning, overexpression vector construction and recombinant strain overexpression, and analysis finds that under salt stress, compared with a wild type, the root length of a plant with the overexpressed CeMYB154 gene is obviously increased, the growth vigor is better, and the growth rate of the plant with the overexpressed CeMYB154 gene is higher. According to the present invention, the content of MDA and H2O2 is significantly reduced, and the activity of CAT, POD and SOD antioxidant enzymes is significantly enhanced, such that the overexpressed CeMYB154 gene can enhance the salt tolerance and the active oxygen scavenging ability of the plant, and the overexpressed CeMYB154 gene is proved to be the salt tolerance positive regulation factor; the CeMYB154 gene can be used in the field of salt-tolerant stress-resistant breeding of cyperus esculentus, and provides an important gene resource for salt-tolerant molecular breeding.

The invention discloses application of a rice gene OsPILS1 in regulation and control of rice yield, and relates to the technical field of rice transgenosis. According to the invention, after the gene OsPILS1 is over-expressed in rice 9311, the number of secondary branches and the number of grains per ear are obviously increased; an editing vector containing an OsPILS1 gene editing site is transferred into rice 9311 to carry out knockout editing on the gene, and phenotypic statistics show that the number of secondary branches of transgenic rice is obviously reduced, and the number of grains per ear and the yield of a single plant are also obviously reduced. The results show that the OsPILS1 gene can obviously increase the number of grains per panicle by regulating and controlling the development of secondary branches of rice, so that the rice yield is regulated and controlled. Therefore, the rice yield can be effectively regulated and controlled by regulating and controlling the expression level of the OsPILS1 gene in rice plants, and the OsPILS1 gene has a huge application prospect in scientific research of breeding new high-yield rice varieties by molecular breeding.

The present invention discloses a gene, GmCDK8, that negatively regulates soybean resistance to Phytophthora spp., and its applications. The disclosed GmCDK8 knockout soybean plants exhibit enhanced resistance to the fungus. Specifically, the results of the soybean cultivar "Williams 82," which lacks GmCDK8, under Phytophthora stress, including root lesion length, leaf lesion area, Phytophthora accumulation, and infected necrotic area on leaves, indicate that knocking out GmCDK8 significantly improves plant resistance to Phytophthora spp. Genetic engineering of this gene for plant resistance to Phytophthora root rot not only provides an important genetic resource for plant molecular breeding but also offers an effective means for achieving high plant yields.

The invention discloses a method for improving citrus canker resistance through a CsAHL15 gene and application of the CsAHL15 gene, and relates to the field of agricultural biological genes. A method for improving citrus canker resistance through CsAHL15 comprises the following steps that 1, a citrus CsAHL15 coding sequence is cloned, and the CsAHL15 coding sequence is shown as SEQ ID No.1; (2) constructing a CsAHL15 overexpression vector; and (3) CsAHL15 overexpression vector transformation citrus, and identification is carried out to obtain a transgenic plant with improved canker resistance. According to the invention, the citrus CsAHL15 coding sequence is cloned, the overexpression vector is constructed, and then the citrus is transformed, so that the canker attack degree of the obtained transgenic plant can be maximally reduced to 40.09% of that of the existing citrus, the canker attack degree can be obviously reduced, and the scab area can be reduced. In conclusion, the method is a potential biological engineering technology for improving the citrus canker resistance, and has a great value for citrus canker resistance molecular breeding.

The invention discloses a PDE11A gene of litopenaeus vannamei, an SNP (Single Nucleotide Polymorphism) molecular marker combination, a detection primer and application of the PDE11A gene. The nucleotide sequence of the PDE11A gene of the litopenaeus vannamei is SEQ ID NO. 1. The SNP molecular marker combination is located on the SEQ ID NO.1 sequence, and the SNP molecular marker combination comprises a first SNP molecular marker and a second SNP molecular marker; the first SNP molecular marker is located at the fourteenth site from the 5'end of a sequence shown as SEQ ID NO.2, and the polymorphism is A / G; the second SNP molecular marker is located at the 217th site from the 5'end of the sequence shown as SEQ ID NO.3, and the polymorphism is A / G. The AA / AA genotype in the SNP molecular marker combination is used for selecting parents, so that the growth speed of a breeding population can be increased, an innovative tool is provided for molecular breeding of litopenaeus vannamei, the breeding period is shortened, and industrial development is assisted.

The invention discloses an RcWRKY29 gene and application of the RcWRKY29 gene in enhancing the resistance of Chinese roses to gray mold. The nucleotide sequence of the RcWRKY29 gene is shown as SEQ ID NO: 1. Researches find that the gene is specifically and highly expressed in Chinese rose petals, and the expression quantity is remarkably increased within 24-48 hours after botrytis cinerea infection. After RcWRKY29 expression is instantaneously inhibited through a virus-induced gene silencing technology, the scab area of Chinese rose petals is increased, and the disease resistance is remarkably reduced; otherwise, overexpression of the gene can significantly enhance disease resistance and reduce scab area and cell damage. The invention provides a recombinant vector containing RcWRKY29, an engineering bacterium and a detection kit, and develops a method for enhancing the disease resistance of Chinese rose based on an agrobacterium-mediated transformation technology. The invention provides a new target for Chinese rose gray mold resistance molecular breeding, can effectively reduce postharvest disease loss, and has important economic value and popularization and application prospects.

The invention relates to the field of plant gene map-based cloning and molecular breeding. Specifically, the invention provides a nucleic acid molecule and a polypeptide, and also provides a recombinant vector, a host cell and a plant or a part, a seed, a cell or a progeny thereof containing the nucleic acid molecule. Furthermore, the invention also provides a method for preparing the transgenic plant, and a method for regulating and controlling the nitrogen utilization efficiency, nitrate absorption or transport, biomass and / or yield of the plant.

The invention relates to the technical field of crop molecular genetic markers, and discloses an InDel molecular marker of avena nuda and application thereof.The molecular marker is a group of 314bp deletion variation and 2bp insertion variation in the 610439484-610440026 interval of the 5C chromosome position of a reference genome of an avena nuda variety OT3098, the nucleotide sequence of the deletion variation is shown as SEQ ID NO.1, the nucleotide sequence of the insertion variation is shown as SEQ ID NO.2, and the nucleotide sequence of the insertion variation is shown as SEQ ID NO.3. The nucleotide sequence of the insertion variation is 5 '-AA-3'. The molecular marker provided by the invention can realize stable detection in agarose gel electrophoresis by combining a specific primer pair (SEQ ID NO.2-3) with an optimized touchdown PCR (Polymerase Chain Reaction) program, has the advantage of being not influenced by a tissue part and a growth period, and provides an efficient and reliable technical means for oat germplasm identification, seed purity detection and molecular breeding.

The invention belongs to the technical field of molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to a goat body height character and application of the SNP molecular marker. Stable SNP sites SNP01, SNP02, SNP03, SNP04 and SNP05 related to the goat body height character are screened out through whole genome association analysis, the dominant genotype of the SNP01 is GG, the dominant genotype of the SNP02 is TT, the dominant genotype of the SNP03 is TT and TC, the dominant genotype of the SNP04 is AA, and the dominant genotype of the SNP05 is CC. By utilizing the SNP molecular marker provided by the invention, the body height character of the goat can be predicted, a scientific basis is provided for molecular breeding of the goat, the efficiency of genetic improvement of the goat can also be improved, and a new molecular marker resource is also provided for genetic breeding of other livestock.

The invention discloses a gene GmCDK8 for negative regulation and control of phytophthora sojae resistance and application of the gene GmCDK8. GmCDK8-knocked-out soybean plants disclosed by the invention enhance the resistance of soybeans to phytophthora sojae; the results of root scab length, leaf scab area, phytophthora accumulation amount, leaf infected and necrotic areas and the like of the soybean cultivar Williams 82 without the GmCDK8 under the stress of phytophthora show that the resistance of the plant to the phytophthora can be remarkably improved by knocking out the GmCDK8. Genetic engineering improvement of plant phytophthora root rot resistance is carried out through the gene, important gene resources are provided for plant molecular breeding, and an effective means is provided for high yield of plants.

The invention relates to the technical field of plant genetic engineering, in particular to a promoter and application thereof in orchid plants. The invention provides an orchid endogenous promoter which is a powerful constitutive promoter and can drive a target gene to be powerfully expressed in various tissues such as roots, stems, leaves and flowers of an orchid. Compared with a CaMV 35S promoter, the promoter has the advantages that the driving efficiency of the gene in orchid plants is greatly improved, an important tool is provided for molecular breeding of plants, and the promoter has a relatively good application prospect.

The invention belongs to the technical field of plant genetic engineering molecular breeding, and particularly relates to application of wheat TabHLH93 protein or a coding gene thereof in regulation and control of plant grain development. According to the invention, a binary expression vector capable of realizing ectopic expression of the TabHLH93 gene is constructed and is genetically transformed into common wheat to obtain a progeny with high expression of the TabHLH93 gene, so that overexpression of the TabHLH93 gene is realized, and it is verified that the TabHLH93 has a function of regulating and controlling the size of wheat grains and also has an important regulation and control effect on agronomic characters such as wheat plant height, tiller number, spike length and thousand seed weight. A candidate gene with an important application prospect is provided for wheat high-yield genetic improvement, and the gene has important significance for guaranteeing grain safety.