1306 results about "Molecular breeding" patented technology

The invention relates to the technical field of bioinformatics, and provides a visual analysis method and system for a rice multi-tissue single cell expression profile. The method comprises the following steps: comparing sequencing data of an original single cell transcriptome of a rice tissue to obtain a standardized transcriptome data set; performing batch effect correction and integration on the standardized transcriptome data set to obtain a whole plant expression matrix; performing cell type annotation on the whole plant expression matrix to obtain a cell type annotation system; carrying out visual dimension reduction processing on the whole plant expression matrix fused with the cell type annotation system, and carrying out co-expression network construction to obtain a modular tissue correlation analysis model; and establishing an interaction end based on the module organization correlation analysis model, and realizing data visualization analysis through the interaction end. The invention provides a one-stop analysis platform for rice cell heterogeneity research, functional gene mining and molecular breeding.

The invention discloses a KASP molecular marker primer combination for detecting peanut late spot disease resistance and application of the KASP molecular marker primer combination, and belongs to the technical field of peanut molecular breeding. The molecular marker linked with the late spot resistance character can be used for detecting the genotype of peanuts so as to judge whether a material has late spot resistance or not, through high-throughput genotyping detection, manpower and material resources are greatly saved, the detection is not influenced by environmental factors, the result is accurate and reliable, and the molecular marker can be used for early screening of peanut late spot resistance varieties and can be applied to early screening of peanut late spot resistance varieties. The breeding period can be shortened and the breeding efficiency is improved.

The invention discloses a molecular marker closely linked with wheat flag leaf width major QTL and application of the molecular marker in prediction of wheat flag leaf width, and belongs to the technical field of wheat molecular biotechnology and breeding. Wherein the molecular marker is 4BFLW-290, the nucleotide sequences of the molecular marker are as shown in SEQ ID NO: 3 and SEQ ID NO: 4, the specific primer pair of the molecular marker is 4BFLW-290-F / R, and the nucleotide sequences of the specific primer pair are as shown in SEQ ID NO: 1 and SEQ ID NO: 2. The molecular marker 4BFLW-290 provided by the invention fully embodies the flag leaf width major QTL of the wheat variety (line), can quickly and accurately predict whether the wheat variety (line) has the flag leaf width major QTL or not, and provides excellent gene resources and selection tools for wheat yield trait molecular breeding.

The invention belongs to the technical field of plant genetic engineering molecular breeding, and particularly relates to application of wheat TabHLH93 protein or a coding gene thereof in regulation and control of plant grain development. According to the invention, a binary expression vector capable of realizing ectopic expression of the TabHLH93 gene is constructed and is genetically transformed into common wheat to obtain a progeny with high expression of the TabHLH93 gene, so that overexpression of the TabHLH93 gene is realized, and it is verified that the TabHLH93 has a function of regulating and controlling the size of wheat grains and also has an important regulation and control effect on agronomic characters such as wheat plant height, tiller number, spike length and thousand seed weight. A candidate gene with an important application prospect is provided for wheat high-yield genetic improvement, and the gene has important significance for guaranteeing grain safety.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention discloses a GmbZIP36 protein related to aluminum resistance of plants in acid soil as well as a coding gene and application of the GmbZIP36 protein, and belongs to the technical field of bioengineering. The amino acid sequence of the GmbZIP36 protein is shown as SEQ ID NO: 2, 3 or 4, and the nucleotide sequence of the coding gene of the GmbZIP36 protein is shown as SEQ ID NO: 1. The invention finds that the expression of the GmbZIP36 gene is induced by aluminum stress, the relative root elongation and root biomass of arabidopsis thaliana and soybean under the aluminum stress can be obviously improved by over-expressing the gene in a plant, and aluminum accumulation is reduced, so that the aluminum resistance of the plant is enhanced. The invention provides a recombinant vector containing the gene, a host cell and application of the recombinant vector and the host cell in regulating and controlling the aluminum resistance of plants. Meanwhile, the invention also provides a method for producing an aluminum-resistant transgenic plant by using the gene, a kit for detecting the gene or protein and a method for carrying out assisted breeding by using the molecular marker linked with the gene. The invention provides a key gene resource for aluminum-resistant molecular breeding of crops, and has important significance for cultivating new varieties of crops adapting to acid soil.

The invention discloses an application of a BnaMYB7 gene or an encoded protein thereof in regulating and controlling the oil content of rape, and belongs to the fields of genetic engineering and crop genetic breeding. The nucleotide sequence of the BnaMYB7 gene is shown as SEQ ID NO: 1 or SEQ ID NO: 2, an agrobacterium-mediated genetic transformation method is used, an overexpression vector of the BnaMYB7 gene and a CRISPR / Cas9 gene editing vector are respectively transformed into a genome of the rape, the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is obtained, and the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is determined by measuring the oil content of seeds of overexpression and mutant materials. It is found that the BnaMYB7 gene positively regulates the oil content of the rape, so that rape varieties with different oil contents can be obtained by using the gene to perform molecular breeding on the rape. The feed additive is of great significance in relieving grain and oil tension and guaranteeing feed safety.

The invention discloses application of a KASP molecular marker in identification of light yellow flower color traits of muskmelons, and belongs to the technical field of molecular markers. According to the KASP molecular marker for identifying the light yellow flower color character of the muskmelon, a genetic segregation population is constructed by taking a dark yellow flower color muskmelon and a light yellow flower color muskmelon as parents, and a stably inherited marker site chr04: 26634798G / A is obtained through molecular marker technology identification. The marker is closely related to the light yellow flower color character of the muskmelon, and has the characteristics of strong specificity and good stability. The molecular marker provided by the invention can realize rapid genotype detection of a muskmelon material through a high-throughput genotyping system, and the identification result is accurate and reliable and is not influenced by environmental factors. The molecular marker can be applied to molecular marker-assisted selective breeding of melon flower color characters, the breeding efficiency is remarkably improved, the breeding period is shortened, the breeding cost is reduced, and an effective molecular breeding tool is provided for directional improvement of melon flower colors.

The invention provides an hpRNA interference vector for laver and application of the hpRNA interference vector, and belongs to the technical field of genetic engineering and algae biology. According to the hpRNA interference vector, a binary vector pBI121 is used as a skeleton vector, a laver endogenous Actin promoter and a multiple cloning site segment are inserted, the multiple cloning sites allow target gene segments to be cloned in a forward and reverse repetition mode, the target gene segments are spaced by introns, and therefore an efficient hpRNA structure is formed after transcription, and the efficient hpRNA interference vector is obtained. The gene can be directly used for silencing important genes related to growth and development, metabolic regulation, stress response and the like in the laver. The invention further provides a construction method of the hpRNA interference vector and application of the hpRNA interference vector in silencing laver genes and provides a method for silencing laver gene expression, the gene silencing effect is remarkable, experimental results can be repeated, and a powerful tool is provided for molecular breeding and genetic improvement of laver.

The invention discloses application of a gene CsPOD7 in cucumber haploid breeding, and belongs to the technical field of molecular breeding. According to the invention, a parthenogenesis haploid induction gene CsPOD7 is cloned from cucumber, the CsPOD7 is knocked out through a CRISPR-Cas9 gene editing technology, and a cspod7 mutant is used as a male parent to be hybridized with other cucumber materials as a female parent, so that parthenogenesis haploid can be generated. The invention is the first application of the gene CsPOD7 in developing a parthenogenesis haploid induction line in dicotyledon, realizes the application of the haploid induction line based on the gene CsPOD7 in cucumber genetic breeding, accelerates the cucumber breeding process, and has huge potential application value and market prospect.

The invention relates to the technical field of biology, in particular to an SNP molecular marker for identifying upland cotton fiber quality, a KASP primer group and application of the SNP molecular marker and the KASP primer group. The invention provides five SNP (Single Nucleotide Polymorphism) molecular markers XW1-XW5 which are obviously related to fiber quality, and accurate and stable genotyping can be realized through verification. Wherein the fiber length can be remarkably increased by excellent allelotypes corresponding to the markers XW1, XW2, XW4 and XW5; the excellent allelotype corresponding to the marker XW3 can significantly improve the micronaire value, provides a reliable tool for upland cotton fiber quality identification and molecular breeding, and has important application value.

The invention belongs to the technical field of molecular biology and plant molecular breeding, and particularly relates to a mustard core SNP molecular marker set and a screening method and application thereof. The invention provides a core SNP marker set (33) which is subjected to whole genome re-sequencing screening and experimental verification and is suitable for mustard germplasm resource identification and genetic analysis for the first time. The marker set is high in polymorphism and good in stability, covers the whole genome and can effectively distinguish different leaf mustard germplasms. By utilizing the core marker set and the matched KASP primer, the genetic typing of the leaf mustard germplasm resources can be quickly and accurately performed with high throughput, and the defects that the traditional morphological identification is time-consuming, labor-consuming and poor in accuracy are overcome. The marker set can be used for analyzing the genetic diversity, the population structure and the phylogenetic relationship of the leaf mustard germplasm resources, and a molecular basis is provided for collection, preservation and evaluation of the germplasm resources and breeding parent matching.

The invention belongs to the technical field of gene detection and gene molecular breeding, and particularly relates to a meat duck whole genome molecular probe combination based on molecular phenotype screening, a 50K gene chip and application thereof. The molecular probe combination and the gene chip of the marker site combination for meat duck whole genome breeding simultaneously cover 7 representative meat duck varieties and 71 economic characters, have richer polymorphism and higher pertinence in meat duck groups, and are lower in cost and higher in speed compared with high-throughput sequencing detection; the breeding chip is designed according to the growth, feed efficiency, slaughtering, breeding, egg quality and various molecular phenotypes of the meat ducks, and compared with a high-throughput sequencing technology, the breeding chip is higher in seed selection accuracy, has higher breeding value, can be widely applied to breeding genotype detection of the meat ducks, and can be used for detecting the breeding genotypes of the meat ducks. And the method has creative significance in the aspect of meat duck genome selective breeding.

The invention discloses an oat KASP marker, a fingerprint spectrum and application, and relates to the technical field of molecular breeding. The KASP marker comprises 96 SNP (Single Nucleotide Polymorphism) sites, and the position information and the polymorphism of the KASP marker are shown in a table 1. The invention also provides a gene chip for analyzing the KASP marker and an oat variety characteristic fingerprint spectrum constructed by using the KASP marker, and the KASP marker, the gene chip and the oat variety characteristic fingerprint spectrum provided by the invention can be used for screening, identifying and tracing oat quality. The method can be used for identifying the authenticity of the seeds and the genetic relationship between the parent and offspring of the oat, and has important significance for protecting and improving the germplasm resources of the oat.

The invention relates to the field of gene engineering and molecular breeding, and discloses application of a ZmTCP5 gene in regulation and control of heat resistance of corn. According to the invention, three types of knockout mutants of the gene are obtained by using a CRISPR / Cas9 technology, and three overexpression materials are created. At normal temperature (25-28 DEG C), the material has no obvious phenotypic difference with a wild type; however, under high temperature stress (45 DEG C), the ZmTCP5 knockout mutant has enhanced heat resistance, reduced reactive oxygen species (ROS) accumulation, and significantly increased ROS scavenging enzyme activity, chlorophyll content and photochemical efficiency; on the contrary, the ZmTCP5 overexpression strain is more sensitive to heat stress, which indicates that the ZmTCP5 negatively regulates the heat resistance of the corn. The research provides a theoretical basis for analyzing a heat-resistant molecular mechanism of the corn, and also provides an important gene resource for creating a new germplasm of the heat-resistant corn.

The invention relates to the technical field of crossing of bioinformatics, artificial intelligence and plant genomics, and discloses a plant single cell gene expression prediction method, system, device and medium. A plant single cell gene expression prediction model realizes dynamic feature fusion of a DNA sequence and chromatin accessibility signals through a gated cross attention mechanism; the problem that a traditional single-mode model cannot model regulation and control dynamic association is effectively solved, and the result interpretability is enhanced; a hybrid expert system and a load balancing design are adopted to significantly improve the recognition capability of the model for rare cell types, and a decoupling prediction head design supports efficient transfer learning; a DNA long sequence processing mechanism and nucleosome scale feature coding ensure cross-species compatibility; an end-to-end automatic process and a dynamic parameter optimization framework greatly improve the practicability; a'prediction-verification 'closed-loop support system can be constructed for molecular breeding, and high-precision and interpretable prediction of plant single-cell gene expression is realized.

The invention discloses a chip for genome breeding and variety identification of tilapia mossambica. The invention provides a group of SNP (Single Nucleotide Polymorphism) marker combination for tilapia mossambica, which comprises 50000 SNP markers which are respectively SNP1-SNP50000 markers. According to the invention, the SNP marker combination related to economic characters of tilapia mossambica is integrated, representative sites with uniform coverage are selected, a biological probe for site typing is developed, and the site coverage, typing accuracy and GS accuracy of the SNP marker combination are basically consistent with those of re-sequencing; the method can be applied to the aspects of tilapia germplasm resource identification, genetic relationship identification, SNP typing, variety identification, molecular breeding, DNA fingerprint database construction, variety purity detection, germplasm resource genetic analysis, whole genome selective breeding, functional gene positioning, genetic map construction, genetic evolution analysis, whole genome association analysis and the like.

The invention relates to the technical field of biology, in particular to an SSR molecular marker primer group for identifying the variety of herba epimedii, a kit and application. The core primers of the SSR molecular marker disclosed by the invention comprise 823, 840, 841, 842, 854 and 866, and the primers can be used for identifying the herba epimedii arrow leaf No.1, the herba epimedii arrow leaf No.2 and the herba epimedii soft hair No.1. The SSR molecule can be used for researching the genetic diversity, genetic relationship and genetic distance of epimedium, and respectively establishing molecular identity cards of three new epimedium varieties, namely epimedium arrow leaf No.1, epimedium arrow leaf No.2 and epimedium soft hair No.1, so that a foundation is laid for genetic map construction, excellent character marking and the like; meanwhile, a scientific basis is provided for constructing a molecular breeding system of a new variety (line) of herba epimedii in the later period.

The invention discloses a scophthalmus maximus anti-edwardsiella tarda SNP molecular marker and application thereof, and belongs to the technical field of molecular breeding and biology. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID No.1, and the polymorphic site of the SNP molecular marker is A / C. The invention further provides application of the SNP molecular marker in screening of turbots with the Edwardsiella tarda resistant character, and the CC genotype turbots are individuals with the Edwardsiella tarda resistant character. By utilizing the SNP molecular marker provided by the invention, DNA level selection can be carried out in the early stage of fish fries, interference of environmental factors is avoided, the accuracy and efficiency of breeding are remarkably improved, and the breeding period is shortened. The molecular marker is remarkably verified in an independent verification group through linear regression analysis, genotype-phenotype association is stable and reliable, false positive is eliminated, and the molecular marker has a good market application prospect.

The invention discloses an application of an SlWRKY51 gene in improvement of tomato plant disease resistance, and belongs to the technical field of bioengineering. A transgenic positive strain is obtained through construction of an SlWRKY51 gene overexpression vector and agrobacterium-mediated transformation. A botrytis cinerea infection experiment shows that compared with a wild type, the transgenic positive strain shows remarkably enhanced disease resistance after being infected by botrytis cinerea. The further real-time fluorescent quantitative PCR detection data shows that the expression quantities of the PR family and defense-related genes in the transgenic positive strain are remarkably increased, which proves that the SlWRKY51 gene can enhance the disease resistance of the tomato by regulating multiple defense signal pathways, and an important gene resource is provided for tomato disease-resistant molecular breeding.

The invention discloses application of a transcription factor ZmbZIP27 in regulation and control of corn plant height and ear height, and belongs to the technical field of plant genetic engineering and corn molecular breeding. The invention reveals that the bZIP transcription factor family member ZmbZIP27 has an important biological function of regulating and controlling the corn plant height and lodging resistance for the first time. A ZmbZIP27 knockout vector is constructed through a CRISPR / Cas9 gene editing technology, and an obtained homozygous mutant strain shows remarkable plant type improvement characteristics compared with a wild type: the plant height is reduced by 8-12%, and the ear height is reduced by 15-22%. And a new gene target and a breeding strategy are provided for creating a new high-yield lodging-resistant corn variety suitable for mechanical harvesting.

The invention discloses a cherry sugar transporter gene CpSWEET10 and application thereof, and belongs to the technical field of cherry gene engineering. A novel sugar transporter gene CpSWEET10 is obtained through cloning, the nucleotide sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.1, and the coded amino acid sequence of the novel sugar transporter gene CpSWEET10 is shown as SEQ ID NO.2. The gene is highly expressed in cherry fruits, and the content of soluble solids and total sugar in mature fruits is remarkably positively correlated with the expression quantity of the soluble solids and the total sugar; through subcellular localization and sugar transport function defect type yeast mutant functional complementation experimental analysis, the protein coded by the CpSWEET10 gene is localized in a cytoplasmic membrane and has a sucrose transport function; the cherry sugar transporter gene CpSWEET10 disclosed by the invention can be applied to the improvement of the sugar degree character of the fruits, and a new gene resource is provided for molecular breeding for improving the high-sugar quality of the cherry fruits.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker associated with pig growth traits and application. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO: 1, and R in the sequence is C or T; one SNP molecular marker site C / T is arranged at the 289bp position of the sequence of the SNP molecular marker. Based on filled high-quality genotype data, an rMVP software package is used for carrying out whole genome association analysis (GWAS) on the character of corrected 100 kg day age (AGE100) of Duroc pigs, one SNP molecular marker site obviously related to the character is screened out, and a new marker resource is provided for molecular breeding of the growth character of the pigs.

The invention belongs to the technical field of gene engineering, and relates to application of a receptor-like kinase gene MdCIK2 in regulation and control of apple disease resistance. The invention provides an application of a receptor-like kinase gene MdCIK2 in regulation and control of apple disease resistance, which comprises the following steps: a) silencing the receptor-like kinase gene MdCIK2 to improve the resistance of apples to rot diseases; or b) silencing a specific gene segment of the receptor-like kinase gene MdCIK2, and improving the resistance of apples to rot disease. The receptor-like kinase gene MdCIK2 for negative regulation of apple disease resistance provided by the invention can provide important gene resources for apple disease resistance molecular breeding and targeted gene editing.

The invention relates to a gene ZmILR1 for controlling the size of a corn kernel as well as a molecular identification method and application of the gene ZmILR1, and belongs to the technical field of plant genetic breeding and functional gene research. The ZmILR1 gene encodes an IAA-amino acid hydrolase, mutation of the IAA-amino acid hydrolase causes corn kernels to become smaller, and overexpression of the gene can create a corn material with increased kernel length, kernel width and hundred-kernel weight. A functional molecular marker A1A4 is developed according to the sequence difference of the mutant gene and a wild type gene at the DNA level, and can be used for rapid identification and screening of small-grain corn materials. The invention provides a theoretical basis and an application tool for analyzing a molecular mechanism of the corn grain size and developing molecular marker-assisted breeding and hybrid seed breeding, and provides important gene resources and technical support for high-yield and high-quality molecular breeding of corn.

The invention belongs to the technical field of plant molecular breeding, and particularly relates to a KASP molecular marker related to the oil content of soybeans and application of the KASP molecular marker. The molecular marker is based on a soybean Glyma. 13G035200 gene, and targets an SNP (Single Nucleotide Polymorphism) site, which is remarkably associated with the soybean oil content, in the gene. The invention further provides a specific primer combination and a detection method of the molecular marker, soybean genotypes can be rapidly distinguished through PCR amplification and fluorescence signal detection, and high-oil-content soybean individuals can be accurately screened. The molecular marker has the advantages of being high in specificity, efficient in detection, reliable in result and the like, can be applied to soybean molecular marker assisted breeding, germplasm resource screening and high-oil soybean variety cultivation, remarkably shortens the breeding period, reduces the breeding cost, and provides an efficient tool for precise improvement of the soybean oil content character.

The invention discloses a molecular marker InDel20 for identifying soybean oil content, a primer and application of the molecular marker InDel20. The molecular marker InDel20 is an insertion / deletion variation of 321 bp located at the 20th chromosome Chr20: 31728602-31728922 of the soybean, and the nucleotide sequence of the molecular marker InDel20 is as shown in SEQ ID No. 1; wherein the soybean material containing the insertion fragment is high-oil-content soybean, and the soybean material not containing the insertion fragment is low-oil-content soybean. The invention further provides a primer for detecting the molecular marker InDel20 and a method for identifying the content of soybean oil, and the identification accuracy rate is as high as 90.61%. The molecular marker InDel20 can be used for early-stage molecular marker-assisted selection of soybean high-oil characters, is suitable for germplasm resource screening, variety identification and molecular breeding of high-oil soybeans, and has important application value for accelerating polymerization of high-quality alleles and improving breeding efficiency.

The invention discloses a gene PtoXTH30 capable of improving drought resistance and salt resistance of poplar and application of the gene PtoXTH30, the nucleotide sequence of the gene is shown as SEQ ID NO.2, the gene can accurately regulate and control the drought resistance and salt resistance of the poplar, and a key candidate gene is provided for stress-resistant molecular breeding of the poplar. The invention further discloses application of the PtoXTH30 gene for improving drought resistance and salt resistance of the poplar, and the drought resistance and salt resistance of the poplar can be remarkably enhanced. The invention further discloses a method for regulating and controlling drought resistance and salt resistance of the poplar, the drought resistance and salt resistance of the poplar can be accurately regulated and controlled according to needs, the breeding period is effectively shortened, and breeding of new drought-resistant and salt-resistant transgenic varieties is facilitated.

The invention provides an agrobacterium rhizogenes-mediated forsythia suspensa hairy root genetic transformation method and application thereof, and belongs to the technical field of plant genetic engineering. The method comprises the following steps: introducing an expression vector into agrobacterium rhizogenes, and mixing with an infection solution to prepare an agrobacterium rhizogenes infection solution; the forsythia suspensa stem is put into the agrobacterium rhizogenes infection liquid to be infected for 0.5-1 h through a vacuum infiltration method, and the infected forsythia suspensa stem is cut into a matrix to be subjected to dark culture and then continues to grow till hairy roots are generated; the OD600 value of the agrobacterium rhizogenes infection liquid is 0.5 to 1.0. The method is suitable for an agrobacterium rhizogenes mediated genetic transformation system of the fructus forsythiae, and the system provides technical support for functional gene research, medicinal component analysis and molecular breeding of the fructus forsythiae, and has important significance for promoting genetic improvement and resource utilization of the fructus forsythiae.

The application relates to the field of genetic engineering breeding and molecular breeding, and particularly discloses ZmMIR319A Application of a gene in regulating a corn leaf angle. ZmMIR319A The gene nucleotide sequence is shown in SEQ ID NO. 1. The application uses a CRISPR / Cas9 gene editing technology to carry out site-specific knockout of a gene in corn ZmMIR319A, Three different types of gene knockout mutants are created, and ZmMIR319A Overexpression materials are created. It is found that, compared with a control, the leaf angle of a leaf on an ear of the knockout mutant is smaller, and the leaf angle of a leaf on an ear of the overexpression plant is increased, which indicates that ZmMIR319A ZmMIR319A The gene can positively regulate a corn leaf angle. The application lays a theoretical foundation for researching a molecular mechanism of a corn leaf angle, and simultaneously provides new molecular markers and gene resources for molecular design breeding and cultivating new excellent corn germplasm with a small leaf angle and a dense planting characteristic.