1777 results about "Molecular breeding" patented technology

The invention discloses an interpretable machine learning genome prediction method and device, belongs to the technical field of combination of biological breeding, biological information and machine learning, and utilizes an advanced machine learning algorithm to perform parameter optimization in combination with biological prior information. Through processing of multi-source data (genome, transcriptome and epigenetic data), dynamic feature engineering (PCA and PHATE dimensionality reduction) and organic combination of various machine learning models, and an automatic parameter adjustment framework based on a grid search and sparrow search algorithm, genome prediction precision and calculation efficiency are significantly improved; meanwhile, the interpretability of the model is realized based on the SHAP value, the SNP site contribution is quantified, a reference is provided for precise breeding, and the method is suitable for animal and plant molecular breeding and medical genetic analysis, and can accelerate genetic analysis of high-value characters, assist precise breeding decision and disease risk prediction, and promote leap-forward development from experience breeding to intelligent breeding.

The invention relates to the technical field of bioinformatics, and provides a visual analysis method and system for a rice multi-tissue single cell expression profile. The method comprises the following steps: comparing sequencing data of an original single cell transcriptome of a rice tissue to obtain a standardized transcriptome data set; performing batch effect correction and integration on the standardized transcriptome data set to obtain a whole plant expression matrix; performing cell type annotation on the whole plant expression matrix to obtain a cell type annotation system; carrying out visual dimension reduction processing on the whole plant expression matrix fused with the cell type annotation system, and carrying out co-expression network construction to obtain a modular tissue correlation analysis model; and establishing an interaction end based on the module organization correlation analysis model, and realizing data visualization analysis through the interaction end. The invention provides a one-stop analysis platform for rice cell heterogeneity research, functional gene mining and molecular breeding.

The invention discloses a KASP molecular marker primer combination for detecting peanut late spot disease resistance and application of the KASP molecular marker primer combination, and belongs to the technical field of peanut molecular breeding. The molecular marker linked with the late spot resistance character can be used for detecting the genotype of peanuts so as to judge whether a material has late spot resistance or not, through high-throughput genotyping detection, manpower and material resources are greatly saved, the detection is not influenced by environmental factors, the result is accurate and reliable, and the molecular marker can be used for early screening of peanut late spot resistance varieties and can be applied to early screening of peanut late spot resistance varieties. The breeding period can be shortened and the breeding efficiency is improved.

The invention is applicable to the technical field of bioengineering, and provides a coding gene for enhancing iron deficiency stress tolerance of plants and application. According to the soybean GmMYB14 transcription factor disclosed by the invention, the problem that the growth of soybeans is limited in an iron-deficient environment, particularly calcareous soil and soda saline alkali soil, is solved by excavating and applying the encoding gene (the nucleotide sequence is shown as SEQ ID No.1) of the soybean GmMYB14 transcription factor. Experiments prove that after the gene is over-expressed in arabidopsis thaliana and soybean, the tolerance of plants to iron deficiency stress can be remarkably enhanced. The invention provides a key gene resource for molecular breeding: the GmMYB14 effectively promotes the absorption and utilization of plants on iron by regulating and controlling the metabolic pathway of phenylpropane, not only can improve the planting adaptability of soybeans in low-iron soil, but also can improve the yield and quality of the soybeans, provides powerful technical support for enlarging the planting area of the soybeans in iron-deficient soil regions, and has a wide application prospect. The method is of great significance in relieving insufficient soybean productivity in China and improving the self-sufficiency rate.

The invention discloses a mulberry SNP (Single Nucleotide Polymorphism) marker mining and stress resistance character prediction method based on a Transform architecture, and belongs to the crossing field of biotechnology and artificial intelligence. According to the method, mulberry genome sequencing and stress resistance phenotype data are collected, high-credibility SNP is screened through quality filtering and Bayesian statistics, a Transform model containing multiple self-attention mechanisms is constructed after multi-dimensional features are extracted, early stop method training optimization is combined, stress resistance character prediction is achieved, and the key SNP contribution degree is analyzed through feature disturbance. According to the method, the problems of insufficient long-distance feature correlation capture, low prediction precision, poor model interpretability and the like in the traditional technology are effectively solved, the stress resistance character prediction precision and the SNP marker credibility are improved, and an interpretable efficient screening strategy is provided for mulberry molecular breeding.

The invention discloses a gene PtoMYB20 for regulating and controlling salt tolerance and drought resistance of poplars. A nucleotide sequence of the gene comprises a sequence as shown in SEQ ID NO. 1. The gene interference fragment is introduced into a plant to obtain a silent transgenic plant, under the conditions of salt stress, drought stress and salt-drought composite stress, the H2O2 and MDA contents of the silent transgenic plant are lower than those of a wild plant, and the SOD and POD activities of the silent transgenic plant are higher than those of the wild plant, so that the damage degree of the plant is effectively reduced; the method is of great significance in improving salt tolerance and drought resistance of the poplar and relieving damage of adversity stress to forestry yield. The invention also discloses a breeding method of the transgenic poplar with strong salt tolerance and drought resistance, provides a new poplar germplasm which not only improves the stress resistance of the poplar but also does not influence the wood yield, and lays a theoretical and gene resource foundation for the stress-resistant molecular breeding of the poplar.

The invention discloses a molecular marker closely linked with wheat flag leaf width major QTL and application of the molecular marker in prediction of wheat flag leaf width, and belongs to the technical field of wheat molecular biotechnology and breeding. Wherein the molecular marker is 4BFLW-290, the nucleotide sequences of the molecular marker are as shown in SEQ ID NO: 3 and SEQ ID NO: 4, the specific primer pair of the molecular marker is 4BFLW-290-F / R, and the nucleotide sequences of the specific primer pair are as shown in SEQ ID NO: 1 and SEQ ID NO: 2. The molecular marker 4BFLW-290 provided by the invention fully embodies the flag leaf width major QTL of the wheat variety (line), can quickly and accurately predict whether the wheat variety (line) has the flag leaf width major QTL or not, and provides excellent gene resources and selection tools for wheat yield trait molecular breeding.

Provided is the use of overexpression of the auxin transporter PtoPIN1a gene in increasing the biomass of Populus tomentosa. The nucleotide sequence of the auxin transporter PtoPIN1a gene is as shown in SEQ ID No. 1. By means of using a genetic engineering method, wild-type Populus tomentosa is transformed by means of Agrobacterium tumefaciens infection to obtain a tissue-specific PtoPIN1a overexpressing transgenic plant, and subsequently, a plant with a relatively high expression level is selected, by means of positive identification, for phenotype observation. Results show that compared with the wild type in the same period, the PtoPIN1a transgenic plant has an increased plant height, a thicker stem and a significantly increased aboveground biomass. By means of the provided technical cultivation method, a high-biomass high-quality poplar variety is cultivated. The method is of great significance for future molecular breeding to cultivate excellent poplar varieties.

The invention discloses application of a PagBAG8 gene in regulation and control of poplar growth and development, and belongs to the technical field of forestry biological genetic engineering. According to the application of the PagBAG8 gene in regulating and controlling growth and development of the poplar, the nucleotide sequence of the PagBAG8 gene is shown as SEQ ID NO: 1, and the method for cultivating the transgenic poplar through the PagBAG8 gene comprises the following steps that the PagBAG8 gene of the poplar is cloned; the PagBAG8 gene is connected to a vector, and an overexpression vector is obtained; transforming the obtained overexpression vector into agrobacterium to obtain an agrobacterium liquid; and infecting poplar leaves with the obtained agrobacterium liquid to obtain a PagBAG8 gene overexpression plant. The invention provides a new choice for regulating and controlling the growth and development of the poplar, and provides a potential target for molecular breeding improvement of the properties of the poplar.

The invention discloses application of a rice gene OsPILS1 in regulation and control of rice yield, and relates to the technical field of rice transgenosis. According to the invention, after the gene OsPILS1 is over-expressed in rice 9311, the number of secondary branches and the number of grains per ear are obviously increased; an editing vector containing an OsPILS1 gene editing site is transferred into rice 9311 to carry out knockout editing on the gene, and phenotypic statistics show that the number of secondary branches of transgenic rice is obviously reduced, and the number of grains per ear and the yield of a single plant are also obviously reduced. The results show that the OsPILS1 gene can obviously increase the number of grains per panicle by regulating and controlling the development of secondary branches of rice, so that the rice yield is regulated and controlled. Therefore, the rice yield can be effectively regulated and controlled by regulating and controlling the expression level of the OsPILS1 gene in rice plants, and the OsPILS1 gene has a huge application prospect in scientific research of breeding new high-yield rice varieties by molecular breeding.

The present invention discloses a gene, GmCDK8, that negatively regulates soybean resistance to Phytophthora spp., and its applications. The disclosed GmCDK8 knockout soybean plants exhibit enhanced resistance to the fungus. Specifically, the results of the soybean cultivar "Williams 82," which lacks GmCDK8, under Phytophthora stress, including root lesion length, leaf lesion area, Phytophthora accumulation, and infected necrotic area on leaves, indicate that knocking out GmCDK8 significantly improves plant resistance to Phytophthora spp. Genetic engineering of this gene for plant resistance to Phytophthora root rot not only provides an important genetic resource for plant molecular breeding but also offers an effective means for achieving high plant yields.

The invention discloses an RcWRKY29 gene and application of the RcWRKY29 gene in enhancing the resistance of Chinese roses to gray mold. The nucleotide sequence of the RcWRKY29 gene is shown as SEQ ID NO: 1. Researches find that the gene is specifically and highly expressed in Chinese rose petals, and the expression quantity is remarkably increased within 24-48 hours after botrytis cinerea infection. After RcWRKY29 expression is instantaneously inhibited through a virus-induced gene silencing technology, the scab area of Chinese rose petals is increased, and the disease resistance is remarkably reduced; otherwise, overexpression of the gene can significantly enhance disease resistance and reduce scab area and cell damage. The invention provides a recombinant vector containing RcWRKY29, an engineering bacterium and a detection kit, and develops a method for enhancing the disease resistance of Chinese rose based on an agrobacterium-mediated transformation technology. The invention provides a new target for Chinese rose gray mold resistance molecular breeding, can effectively reduce postharvest disease loss, and has important economic value and popularization and application prospects.

The invention relates to the field of plant gene map-based cloning and molecular breeding. Specifically, the invention provides a nucleic acid molecule and a polypeptide, and also provides a recombinant vector, a host cell and a plant or a part, a seed, a cell or a progeny thereof containing the nucleic acid molecule. Furthermore, the invention also provides a method for preparing the transgenic plant, and a method for regulating and controlling the nitrogen utilization efficiency, nitrate absorption or transport, biomass and / or yield of the plant.

The invention relates to the technical field of crop molecular genetic markers, and discloses an InDel molecular marker of avena nuda and application thereof.The molecular marker is a group of 314bp deletion variation and 2bp insertion variation in the 610439484-610440026 interval of the 5C chromosome position of a reference genome of an avena nuda variety OT3098, the nucleotide sequence of the deletion variation is shown as SEQ ID NO.1, the nucleotide sequence of the insertion variation is shown as SEQ ID NO.2, and the nucleotide sequence of the insertion variation is shown as SEQ ID NO.3. The nucleotide sequence of the insertion variation is 5 '-AA-3'. The molecular marker provided by the invention can realize stable detection in agarose gel electrophoresis by combining a specific primer pair (SEQ ID NO.2-3) with an optimized touchdown PCR (Polymerase Chain Reaction) program, has the advantage of being not influenced by a tissue part and a growth period, and provides an efficient and reliable technical means for oat germplasm identification, seed purity detection and molecular breeding.

The invention belongs to the technical field of molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to a goat body height character and application of the SNP molecular marker. Stable SNP sites SNP01, SNP02, SNP03, SNP04 and SNP05 related to the goat body height character are screened out through whole genome association analysis, the dominant genotype of the SNP01 is GG, the dominant genotype of the SNP02 is TT, the dominant genotype of the SNP03 is TT and TC, the dominant genotype of the SNP04 is AA, and the dominant genotype of the SNP05 is CC. By utilizing the SNP molecular marker provided by the invention, the body height character of the goat can be predicted, a scientific basis is provided for molecular breeding of the goat, the efficiency of genetic improvement of the goat can also be improved, and a new molecular marker resource is also provided for genetic breeding of other livestock.

The invention discloses a gene GmCDK8 for negative regulation and control of phytophthora sojae resistance and application of the gene GmCDK8. GmCDK8-knocked-out soybean plants disclosed by the invention enhance the resistance of soybeans to phytophthora sojae; the results of root scab length, leaf scab area, phytophthora accumulation amount, leaf infected and necrotic areas and the like of the soybean cultivar Williams 82 without the GmCDK8 under the stress of phytophthora show that the resistance of the plant to the phytophthora can be remarkably improved by knocking out the GmCDK8. Genetic engineering improvement of plant phytophthora root rot resistance is carried out through the gene, important gene resources are provided for plant molecular breeding, and an effective means is provided for high yield of plants.

The invention relates to the technical field of plant genetic engineering, in particular to a promoter and application thereof in orchid plants. The invention provides an orchid endogenous promoter which is a powerful constitutive promoter and can drive a target gene to be powerfully expressed in various tissues such as roots, stems, leaves and flowers of an orchid. Compared with a CaMV 35S promoter, the promoter has the advantages that the driving efficiency of the gene in orchid plants is greatly improved, an important tool is provided for molecular breeding of plants, and the promoter has a relatively good application prospect.

The invention belongs to the technical field of plant genetic engineering molecular breeding, and particularly relates to application of wheat TabHLH93 protein or a coding gene thereof in regulation and control of plant grain development. According to the invention, a binary expression vector capable of realizing ectopic expression of the TabHLH93 gene is constructed and is genetically transformed into common wheat to obtain a progeny with high expression of the TabHLH93 gene, so that overexpression of the TabHLH93 gene is realized, and it is verified that the TabHLH93 has a function of regulating and controlling the size of wheat grains and also has an important regulation and control effect on agronomic characters such as wheat plant height, tiller number, spike length and thousand seed weight. A candidate gene with an important application prospect is provided for wheat high-yield genetic improvement, and the gene has important significance for guaranteeing grain safety.

The invention relates to the technical field of modern livestock breeding, in particular to application of an MC5R gene in detection of goat growth traits. The invention provides application of an MC5R gene in detection of goat growth traits, a molecular marker related to the goat growth traits exists at the 519bp position of the nucleotide sequence of the MC5R gene, and the polymorphism of the molecular marker is C / T. When the genotype of the polymorphic site contained in the molecular marker is CC, the genotype corresponds to the growth trait relative advantage of the goat to be detected; when the genotype of the polymorphic site contained in the molecular marker is TT, the growth traits of the goat to be detected are relatively inferior. The molecular marker provided by the invention can be applied to identification or prediction of the growth state of the goats, can also be applied to the field of molecular breeding, and judges the genetic potential, seed reservation and seed reproduction of the goats on the basis of analysis of the genotypes of the goats. The invention has great significance for modern livestock breeding of goats.

The invention discloses application of an LRK20 gene in regulation and control of rice disease resistance, a related biological material and a cultivation method. According to the application of the LRK20 gene in regulation and control of rice disease resistance, the sequence of the LRK20 gene is shown as SEQ ID NO.1, and the disease resistance is sheath blight resistance. The amino acid sequence of the protein coded by the LRK20 gene is as shown in SEQ ID NO. 2. The recombinant vector comprises the complementary sequence of the LRK20 gene in the application. The novel gene LRK20 for regulating and controlling the resistance of the rice sheath blight disease is obtained through identification, the sheath blight resistance gene LRK20 can regulate and control the resistance of the rice sheath blight disease, the resistance of the rice to the sheath blight disease can be remarkably weakened by knocking out the gene, and the resistance of the rice to the sheath blight disease can be remarkably enhanced by overexpressing the gene; therefore, the gene has a potential breeding application value in sheath blight resistance molecular breeding.

The invention belongs to the technical field of biology, and particularly relates to application of a soybean GmSRO5 gene in promoting generation of leguminous plant root nodules. A pZP211 overexpression vector is utilized, a transgenic plant is rapidly obtained through agrobacterium tumefaciens-mediated soybean genetic transformation, GmSRO5 is successfully overexpressed, root nodule number statistics is carried out on a chimera transgenic plant, and a result shows that the GmSRO5 gene can positively regulate and control the number of soybean root nodules and has a remarkable effect. The invention proposes that SRO5 can positively regulate and control the soybean nodulation process for the first time, and proves that the soybean GmSRO5 gene overexpression can significantly increase the number of soybean root nodules, and the soybean GmSRO5 gene overexpression can be applied to soybean production and molecular breeding through transgenosis, molecular marking and other means in the future, and has very important application value.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention provides a method for jointly evaluating the cellulose content and the lignin content of poplar wood based on a Pto-CDPK4 haplotype and application of the method, and belongs to the technical field of forest molecular breeding. According to the method disclosed by the invention, genotypes of mutation sites at the 248th site, the 255th site, the 297th site, the 363th site and the 367th site of a Pto-CDPK4 promoter region are detected, and according to a haplotype combination of the five sites, the cellulose and lignin contents of the poplar wood are accurately predicted and jointly evaluated; therefore, excellent individuals with high cellulose content and low lignin content in the wood can be accurately and efficiently screened in the early growth stage of the poplar, the breeding period can be remarkably shortened, and the breeding efficiency can be improved.

The invention discloses a GmbZIP36 protein related to aluminum resistance of plants in acid soil as well as a coding gene and application of the GmbZIP36 protein, and belongs to the technical field of bioengineering. The amino acid sequence of the GmbZIP36 protein is shown as SEQ ID NO: 2, 3 or 4, and the nucleotide sequence of the coding gene of the GmbZIP36 protein is shown as SEQ ID NO: 1. The invention finds that the expression of the GmbZIP36 gene is induced by aluminum stress, the relative root elongation and root biomass of arabidopsis thaliana and soybean under the aluminum stress can be obviously improved by over-expressing the gene in a plant, and aluminum accumulation is reduced, so that the aluminum resistance of the plant is enhanced. The invention provides a recombinant vector containing the gene, a host cell and application of the recombinant vector and the host cell in regulating and controlling the aluminum resistance of plants. Meanwhile, the invention also provides a method for producing an aluminum-resistant transgenic plant by using the gene, a kit for detecting the gene or protein and a method for carrying out assisted breeding by using the molecular marker linked with the gene. The invention provides a key gene resource for aluminum-resistant molecular breeding of crops, and has important significance for cultivating new varieties of crops adapting to acid soil.

The invention discloses an application of a BnaMYB7 gene or an encoded protein thereof in regulating and controlling the oil content of rape, and belongs to the fields of genetic engineering and crop genetic breeding. The nucleotide sequence of the BnaMYB7 gene is shown as SEQ ID NO: 1 or SEQ ID NO: 2, an agrobacterium-mediated genetic transformation method is used, an overexpression vector of the BnaMYB7 gene and a CRISPR / Cas9 gene editing vector are respectively transformed into a genome of the rape, the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is obtained, and the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is determined by measuring the oil content of seeds of overexpression and mutant materials. It is found that the BnaMYB7 gene positively regulates the oil content of the rape, so that rape varieties with different oil contents can be obtained by using the gene to perform molecular breeding on the rape. The feed additive is of great significance in relieving grain and oil tension and guaranteeing feed safety.

The invention provides a wheat drought-tolerant gene TaTZF1 and application thereof in plant drought tolerance, and belongs to the technical field of gene engineering. The invention provides a wheat drought-enduring gene TaTZF1. The amino acid sequence of a protein encoded by the wheat drought-enduring gene TaTZF1 is as shown in SEQ ID No. 2. The wheat drought-enduring gene TaTZF1 is subjected to induced expression by drought, ABA and salt stress, the wheat drought-enduring gene TaTZF1 is overexpressed and transformed into wheat in an overexpression mode, and it is found that the drought tolerance of a transgenic plant with overexpressed TaTZF1 is remarkably improved compared with that of a wild type plant; after the wheat drought-tolerant gene TaTZF1 in wheat is knocked out, the drought tolerance is obviously reduced. It is proved that the wheat drought-resistant gene TaTZF1 positively regulates the drought resistance of wheat, gene resources are provided for wheat molecular breeding, and the wheat drought-resistant gene TaTZF1 has great significance in improvement of wheat drought-resistant genetic improvement.

The invention discloses application of a KASP molecular marker in identification of light yellow flower color traits of muskmelons, and belongs to the technical field of molecular markers. According to the KASP molecular marker for identifying the light yellow flower color character of the muskmelon, a genetic segregation population is constructed by taking a dark yellow flower color muskmelon and a light yellow flower color muskmelon as parents, and a stably inherited marker site chr04: 26634798G / A is obtained through molecular marker technology identification. The marker is closely related to the light yellow flower color character of the muskmelon, and has the characteristics of strong specificity and good stability. The molecular marker provided by the invention can realize rapid genotype detection of a muskmelon material through a high-throughput genotyping system, and the identification result is accurate and reliable and is not influenced by environmental factors. The molecular marker can be applied to molecular marker-assisted selective breeding of melon flower color characters, the breeding efficiency is remarkably improved, the breeding period is shortened, the breeding cost is reduced, and an effective molecular breeding tool is provided for directional improvement of melon flower colors.

The invention provides an hpRNA interference vector for laver and application of the hpRNA interference vector, and belongs to the technical field of genetic engineering and algae biology. According to the hpRNA interference vector, a binary vector pBI121 is used as a skeleton vector, a laver endogenous Actin promoter and a multiple cloning site segment are inserted, the multiple cloning sites allow target gene segments to be cloned in a forward and reverse repetition mode, the target gene segments are spaced by introns, and therefore an efficient hpRNA structure is formed after transcription, and the efficient hpRNA interference vector is obtained. The gene can be directly used for silencing important genes related to growth and development, metabolic regulation, stress response and the like in the laver. The invention further provides a construction method of the hpRNA interference vector and application of the hpRNA interference vector in silencing laver genes and provides a method for silencing laver gene expression, the gene silencing effect is remarkable, experimental results can be repeated, and a powerful tool is provided for molecular breeding and genetic improvement of laver.

The invention belongs to the technical field of SNP molecular markers, and particularly relates to an SNP molecular marker related to the breast depth character of a goat, a product for detecting the SNP molecular marker and application of the SNP molecular marker. The SNP molecular marker has remarkable correlation with the goat chest depth character, a goat chest depth molecular marker database is enriched, research finds that in the goat breeding process, the SNP molecular marker can be used for predicting the goat chest depth character, and for an SNP01 molecular marker, an SNP02 molecular marker, an SNP03 molecular marker, an SNP04 molecular marker and an SNP05 molecular marker, the SNP molecular marker can be used for predicting the goat chest depth character. The homozygous genotype individuals with the advantages can be respectively selected and reserved, potential application value is achieved for goat chest depth character molecular breeding, and a new molecular marker resource is provided for goat genetic breeding.

The invention relates to application of receptor-like kinase MdRLK2 in comprehensive resistance breeding of apples. After the receptor-like kinase MdRLK2 is instantaneously overexpressed in the apple tissue culture seedling Gal-3 and the apple Gala branch, it is found that the improvement of the expression quantity of the receptor-like kinase MdRLK2 effectively enhances the capability of resisting alternaria leaf spot, anthrax leaf blight, brown spot, rot disease and ring spot of apples; therefore, a molecular means for improving the comprehensive disease resistance of the apples is developed by overexpressing the apple receptor-like kinase MdRLK2, and meanwhile, a theoretical basis is provided for molecular breeding of the apples.