252 results about "Molecular breeding" patented technology

This invention relates to the fields of genomics, molecular biology, bioinformatics, and genome-wide selection breeding, specifically a 20k liquid-phase breeding chip for the whole genome of *S. esculenta* and its applications. The liquid-phase chip contains background SNPs and functional SNPs located on the *S. esculenta* reference genome; wherein the background SNPs are uniformly distributed within the genome; and the functional SNPs are associated with important economic traits of *S. esculenta*; these important economic traits include one or more of the following: saponin content, polysaccharide content, and heat tolerance. The chip can be applied to the assessment of genetic diversity in *S. esculenta*, identification of germplasm resources and phylogenetic relationships, genome-wide association analysis of important economic traits, and genome-wide selection breeding. This chip has advantages such as high throughput, high region coverage, high locus detection rate, and high flexibility, providing powerful tool support for molecular breeding of *S. esculenta*.

This invention relates to the field of plant molecular breeding technology, specifically to a KASP molecular marker PH5-KASP based on the SNP site of the maize Zm00001eb259660 gene and its application. It provides a KASP molecular marker targeting the SNP site at 226029364 bp on maize chromosome 5, which is a functional missense mutation c.485C>T in the coding region of the Zm00001eb259660 gene. The marker consists of two allele-specific forward primers carrying different fluorescent tags and one universal reverse primer, stably distinguishing between C:C and T:T homozygous genotypes, where C:C corresponds to higher plant height and T:T corresponds to lower plant height. This invention offers high marker genotyping accuracy, simple and low-cost detection, and is suitable for large-scale breeding sample detection. It can be used for early identification of maize plant height traits, germplasm screening, and assisted breeding, significantly improving the efficiency of ideal maize plant architecture improvement.

This invention belongs to the field of plant and component detection, specifically relating to a method for detecting anthocyanin content in different varieties of Hibiscus mutabilis and its application. The technical problem solved by this invention is to provide a method for detecting anthocyanin content in different varieties of Hibiscus mutabilis. The content detection method of this invention includes: A. Extraction and processing of Hibiscus mutabilis anthocyanin samples: mainly including optimization of flower pretreatment and extraction methods. B. Detection of the content of the extracted Hibiscus mutabilis anthocyanins using high-performance liquid chromatography (HPLC): mainly including optimization of parameters such as mobile phase type, mobile phase ratio, detection wavelength, and injection volume. This detection method can be applied to the correlation study between Hibiscus mutabilis flower color and components, for molecular breeding of Hibiscus mutabilis flower color, and for detecting the anthocyanin content of different Hibiscus mutabilis varieties to identify varieties that are difficult to distinguish.

The application belongs to the technical field of plant genetic engineering, and particularly relates to application of a potato StSS3-like gene in anthocyanin synthesis regulation, wherein the StSS family gene StSS3-like is identified from different potato flesh colors, sequence characteristics and expression patterns of the StSS3-like gene are determined, and through tobacco transient expression, stable genetic transformation, yeast one-hybrid and double luciferase experiments, it is confirmed that the gene has an inhibitory effect on anthocyanin biosynthesis, StAN1, StMYB3 and StHOS15 are determined as upstream activating regulatory factors of the gene, and StbHLH1 can weaken the activating effect of StMYB3 on the gene. The application discloses a molecular mechanism of StSS3-like participating in anthocyanin synthesis, fills a blank of potato StSS family function research, and provides new gene resources and theoretical basis for color potato molecular breeding and functional food development.

The application discloses a SNP site and KASP marker combination related to contents and proportions of ginsenosides Rg1 and Rb1 in Panax notoginseng and application thereof, and belongs to the technical field of medicinal plant molecular breeding and quality evaluation. In view of the problems of lack of molecular markers synchronously related to contents and proportions of ginsenosides Rg1 and Rb1 in Panax notoginseng and long breeding cycle in the prior art, nine SNP site combinations located on a Chr11 chromosome of Panax notoginseng genome are screened, and a KASP marker combination and primer combination are developed based on four sites of SNP3-SNP6 among them. The KASP marker combination has strong specificity, is convenient to detect, has high throughput, is low in cost, and can accurately identify the contents and proportions of Rg1 and Rb1 in Panax notoginseng samples. The application can be used for Panax notoginseng germplasm resource screening and molecular marker assisted breeding, early identification of target traits is realized, the breeding cycle is shortened, the breeding efficiency is improved, and technical support is provided for directional cultivation of Panax notoginseng high-quality varieties.

This invention provides a gene related to the glossy leaf trait in Rugao black cabbage and its application, belonging to the field of molecular genetics and breeding technology. Specifically, this invention identifies the gene controlling the glossy leaf trait in 'Rugao black cabbage', confirms that this trait is controlled by a single dominant gene, and successfully locates the candidate gene. BraA02g02617P This study confirmed the association between the gene mutation and the glossy leaf trait. The discovery of the new dominant gene controlling glossy leaves greatly improves the convenience of molecular breeding of cruciferous plants, especially Brassica species, and has promising application prospects.

This invention discloses a cultivated rice from Africa pi- cnv9 The application of genes in improving rice disease resistance belongs to the field of genetic engineering technology. This invention has screened a gene associated with rice blast resistance. pi-cnv9 The gene, whose nucleotide sequence is shown in SEQ ID NO.1, was used in this invention to construct complementary transgenes for *Rhizophora flavescens* and *Rhizophora esculenta*, respectively. pi-cnv9 Rice plants with complementary genes. Inoculation experiments were conducted on wild-type and transgenic plants, and the results demonstrated that complementary transgenic... pi-cnv9 Genetically modified plants are more resistant to disease than wild-type plants; indicating that... pi-cnv9 Genes that positively regulate the disease resistance of rice can be used as target genes for genetic improvement and molecular breeding of rice resistance to rice blast, which is of great significance for breeding rice varieties with improved disease resistance.

The application belongs to the field of new application of functional proteins and mutants thereof, and discloses application of sugarcane ScCBS protein and key domain deletion mutants thereof. The nucleotide sequence of the sugarcane ScCBS protein is shown as SEQ ID NO:1, and the amino acid sequence is shown as SEQ ID NO:2. The key domain deletion mutants of the ScCBS protein are ScCBS△motif1, ScCBS△motif3, ScCBS△motif4 and ScCBS△motif9, which respectively lack 28, 50, 49 and 29 amino acids. The application proves that the ScCBS protein can interact with SCSMV P1 and inhibit the RNA silencing suppressor activity by means of yeast two-hybrid, two-molecular fluorescence complementary experiment and agrobacterium transient expression system; the four mutants all lose the interaction and inhibition activity. Therefore, the ScCBS protein and the key domain deletion mutants thereof can be used for cultivating excellent SCSMV-resistant materials, and have wide application prospects in sugarcane disease-resistant molecular breeding.

The application discloses a use of a wheat TaCIPK19-3D gene and a method for improving salt tolerance of crops. The wheat TaCIPK19-3D gene is taken as the object, and it is found that wild type (WT), a transgenic line overexpressing the TaCIPK19-3D gene and an oscipk19 gene knockout mutant plant all show obvious phenotype differences after salt stress. The transgenic line overexpressing the TaCIPK19-3D gene shows obvious salt tolerance phenotype, and the leaves of the transgenic line overexpressing the TaCIPK19-3D gene are greener and more active than those of the wild type and the mutant after salt treatment. The leaves of the oscipk19 gene knockout mutant line of a rice homologous gene are more withered than those of the wild type after salt treatment. The results show that the TaCIPK19-3D gene can positively regulate the salt tolerance of plants, and can be applied to the breeding of wheat salt-tolerant varieties through molecular breeding and other technologies.

This invention provides EsPTI2 The application of genes in regulating plant leaf number falls under the field of biotechnology. This invention is the first to discover that overexpression of genes in plants... EsPTI2 The gene can increase the number of leaves in rice and wheat, promote the synthesis of more carbohydrates and other nutrients, provide support for plant growth and development, and thus help biomass accumulation and yield increase. It fills the technical gap in high-yield plant type improvement of this species, provides a theoretical basis and technical reference for molecular design breeding of other grass forage grasses, and promotes the upgrading of forage grass breeding from traditional phenotypic selection to precision molecular breeding.

The application belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker closely linked to a soybean seed protein content QTL qPro14 and application. A seed protein content QTL qPro14 is identified on chromosome 14 of soybean by using whole genome association analysis. A significant SNP associated with the QTL is located at the 1,360,542th base of chromosome 14 of a reference genome Glycine_max_v2.1, and can explain 0.32% of phenotypic variation. A PARMS marker developed by using the SNP is clear in genotyping and simple in operation, and is suitable for molecular breeding of soybean seed protein content.

This invention, entitled "An Optimized RNA Stabilizing Element and Its Application in Enhancing Transient Expression in Tobacco," belongs to the field of plant genetic engineering technology. The technical problem to be solved is to provide an optimized RNA stabilizing element capable of enhancing transient expression in tobacco and its application in this process. The key technical solution is to provide a method for enhancing the transient expression level of tobacco, comprising: introducing an optimized RNA stabilizing element into the expression vector of the target gene, wherein the optimized RNA stabilizing element is a 5′UTR core motif containing the nucleotide sequence shown in SEQ ID NO:1. This method effectively overcomes the bottleneck of exogenous gene expression in plants, providing a highly efficient expression enhancement tool for plant bioreactors and molecular breeding.

The application discloses a gene for regulating grape fruit ripening and application thereof, and belongs to the technical field of plant genetic engineering. The VvNAC86 gene is cloned from grape fruit, and the nucleotide sequence is shown as SEQ ID NO:1. The overexpression vector of the gene is constructed, the agrobacterium is transformed by heat shock, and the engineering strain available for plant transformation is obtained. Genetic transformation is carried out on grape fruit, tobacco and grape callus respectively. The results show that overexpression of VvNAC86 can make grape fruit color 7-10 days in advance, and the soluble solid content is increased by about 40%; the transgenic tobacco has a significantly increased plant height, and the initial flowering period is in advance; the transgenic grape callus matures faster, the color is deeper, and the gray value is significantly lower than that of the wild type. It is proved that VvNAC86 has a function of significantly promoting plant maturation, and the effect is stable in multiple systems, so the application can be applied to grape early maturation molecular breeding, and the breeding cycle can be effectively shortened, the cultivation cost can be reduced, and the economic benefit can be improved.

The application belongs to the technical field of apple molecular breeding, and particularly relates to application of a detection reagent of a molecular marker related to apple anthracnose leaf blight resistance, the molecular marker being Del772, Del231 and SNP947, the nucleotide sequence of Del772 being shown as SEQ ID NO. 1, the nucleotide sequence of Del231 being shown as SEQ ID NO. 4, and the nucleotide sequence of SNP947 being shown as SEQ ID NO. 5, the application further develops the detection reagent of the molecular markers Del772, Del231 and SNP947, the detection reagent can detect or identify the resistance of apples to anthracnose leaf blight, and a method for identifying the resistance of apples to anthracnose leaf blight is developed, thereby providing a new way for breeding disease-resistant apples.

This invention belongs to the field of molecular breeding technology and provides a method based on... YIPF1 Molecular breeding methods and applications for inhibiting egg production decline in laying hens using the eQTL gene. This invention combines molecular markers with... YIPF1 Gene expression levels have a regulatory role; the combined molecular markers are located at Chr8:24801464 and Chr8:24802450 loci in the chicken genome, respectively. This invention breaks through the traditional single-trait research paradigm, integrating multidimensional omics and phenotypic data to identify genes from a systems biology perspective. YIPF1 The gene and its regulatory site combination, Chr8:24801464 and Chr8:24802450, serve as key targets in the process of tissue functional decline in laying hens. This gene possesses cross-tissue, dynamically correlated early warning characteristics, providing a reliable tool for early assessment and precise breeding of anti-aging traits in laying hens, and has significant application value for achieving the goal of an ultra-long laying cycle.

The application discloses a high-throughput KASP molecular marker significantly related to plant height of Gossypium hirsutum and application, and belongs to the technical field of cotton molecular breeding. The KASP marker is successfully developed based on cotton plant type related genes and resequencing data of germplasm resources. Through genotyping and phenotype correlation analysis in 246 natural Gossypium hirsutum populations, four KASP markers (PH-TK1-PH-TK4) significantly related to plant height in three environments are screened. The regulation effect and aggregation effect of the markers on plant height are verified in a separation population. Experiments prove that the KASP marker can be used for high-throughput and accurate genotyping of plant height in early cotton breeding, and provides an effective tool for molecular marker assisted selection of cotton ideal plant type, and helps to accelerate the breeding process of cotton varieties suitable for mechanical harvesting.

This invention discloses the application of the OsSMP3 gene in the control of rice brown planthopper, specifically disclosing that the OsSMP3 gene (LOC_Os11g05170) negatively regulates rice resistance to brown planthopper. By targeting this gene using CRISPR / Cas9 gene editing technology, a loss-of-function mutant was obtained. Compared to the wild type, the knockout mutant showed significantly enhanced resistance to brown planthopper, specifically manifested in increased rice plant survival rate. This invention provides new gene resources and targets for molecular breeding of rice resistant to brown planthopper.

This invention discloses Chinese kale BoaFLZ35 The application of genes in promoting vegetative growth of plants. This invention utilizes a gene-based approach to construct a *Gynostemma pentaphyllum* plant. BoaFLZ35 Heterologous overexpression of the gene in Arabidopsis thaliana was used to observe and analyze its effects on vegetative growth traits such as root elongation and rosette leaf morphology in transgenic plants. The results showed that heterologous overexpression... BoaFLZ35 This gene can significantly increase root cell length, thereby promoting the elongation of the Arabidopsis taproot; simultaneously, it can also increase leaf cell volume, resulting in significantly larger rosette leaves in transgenic plants. Therefore, it is evident that... BoaFLZ35 This is an important gene in the FLZ family of plants that positively regulates vegetative growth. This invention provides new insights into the function of FLZ family genes in the genus Brassica and also provides important candidate genes for high-yield molecular breeding of Brassica vegetables such as Chinese kale.

This invention discloses a method for detecting QTLs of resistance to powdery mildew. QPm.jaas-4BL This invention relates to a molecular marker and its application. The SNP molecular marker comprises the nucleotide sequence shown in SEQ ID NO:1, which contains the SNP site AX-111039690 located at position 151 bp and exhibiting G / C polymorphism. Furthermore, this invention discovered a powdery mildew resistance locus located on the long arm of wheat chromosome 4B through DH population mapping analysis of the wheat hybrids Shanhongmai and Yangmai 15. QPm.jaas-4BL Furthermore, the associated SNP locus AX-111039690 was converted into a PCR marker, which can be used to detect powdery mildew resistance QTLs. QPm.jaas-4BL QPm.jaas-4BL QPm.jaas-4BL QPm.jaas-4BL QPm The genotype was determined and used for molecular breeding to resist powdery mildew.

This invention relates to the field of bioengineering technology, and more particularly to a gene for identifying salt tolerance in upland cotton, GhNRX16, and its molecular marker. The CDS sequence of the upland cotton gene GhNRX16 is shown in SEQ ID NO.1 of the sequence listing. This invention uses RNA-seq technology to screen and identify this salt tolerance-related gene in the transcriptome data of upland cotton under salt stress treatment. Furthermore, the GhNRX16 sequence was obtained through gene cloning, and a transient silencing vector based on VIGS (virus-induced gene silencing) technology was successfully constructed. Experiments using VIGS-mediated gene silencing combined with salt stress treatment showed that silencing the GhNRX16 gene significantly reduced the plant's tolerance to salt stress, demonstrating that normal expression of this gene directly contributes to ensuring the plant's salt stress resistance. These results confirm the crucial role of the GhNRX16 gene in regulating cotton salt tolerance, providing important gene resources and theoretical basis for molecular breeding of salt-tolerant cotton.

The application discloses a chrysanthemum constitutive high-expression promoter and application thereof in gene editing. The nucleotide sequence of the promoter CmUbi is shown as SEQ ID NO. 4. Through double luciferase reporter experiments and stable genetic transformation experiments, it is proved that the expression activity of the CmUbi promoter in chrysanthemum protoplast and callus is significantly higher than that of commonly used CaMV 35S, AtRPS5a and corn ZmUbi promoters. A CRISPR / Cas9 gene editing vector is constructed by using the promoter, and a key gene of branch of hexaploid chrysanthemum is edited BRC1b , and a plurality of allele simultaneous mutation complete editing strains are successfully obtained, and the number of branches of the mutants is significantly increased. The endogenous high-activity promoter of the chrysanthemum provided by the application effectively solves the problems of low expression activity of an existing heterologous promoter in the chrysanthemum and poor gene editing efficiency of a polyploid, and provides an efficient and adaptive expression regulation element for chrysanthemum molecular breeding and functional genomics research.

This invention discloses a combination of SNP molecular markers for sweet potato, an SNP chip, and their applications, relating to the fields of plant biotechnology and plant molecular breeding. The chip contains 16,730 SNP loci located on chromosome 15 of the sweet potato reference genome "Y22". This sweet potato 16K liquid-phase SNP chip, SweetpotatoGBTS16K, is applied to genotyping, variety identification, gene mapping, and genome-wide association analysis of sweet potato varieties. The SNP loci on the liquid-phase chip of this invention were screened from large-scale sweet potato genome resequencing data, totaling 16,730 SNP loci, encompassing associated loci for major agronomic traits such as yield, quality, and resistance in sweet potato, and has broad application prospects in multiple fields of sweet potato breeding.

The present application relates to the technical field of biological genetic engineering, and particularly relates to a kind of identification of plant salt tolerance land cotton gene GhNRX71 And molecular marker, the CDS sequence of the land cotton gene GhNRX71 As shown in SEQ ID NO.1 In the sequence table.The present application is screened and identified in the transcriptome data of land cotton under salt stress treatment by transcriptome sequencing (RNA-seq) technology.The sequence of GhNRX71 Is obtained by further gene cloning means, and the transient silencing vector based on VIGS (virus-induced gene silencing) technology is successfully constructed.The results show that the tolerance of plant to salt stress significantly reduces after silencing GhNRX71 Gene by using VIGS-mediated gene silencing combined with salt stress treatment experiment, which proves that the normal expression of the gene has a direct effect on guaranteeing the salt stress resistance of plant.The above results confirm that GhNRX71 Gene plays a key role in regulating the salt tolerance of cotton, and provides important gene resources and theoretical basis for cotton salt-tolerant molecular breeding.

The application belongs to the technical field of genetic engineering, and particularly relates to application of AtPEN2 protein and / or its coding gene AtPEN2 in preventing cruciferous plant diseases. The application provides application of AtPEN2 protein and / or its coding gene AtPEN2 in preventing cruciferous plant diseases, and the amino acid sequence of the AtPEN2 protein is shown as SEQ ID NO. 1. The application takes Arabidopsis thaliana as a model plant to verify the prevention and treatment effect of overexpression of AtPEN2 protein on clubroot, and the results show that the AtPEN2 protein overexpression plant can improve the resistance to clubroot, indicating that the coding gene AtPEN2 of the AtPEN2 protein can be applied to molecular breeding of clubroot, and provides a new gene resource for molecular breeding of cruciferous crops.

The application relates to the field of molecular breeding, in particular to a molecular marker for identifying interspecific hybrid of brassica rapa and coelonema and application thereof. The molecular marker comprises one pair of brassica rapa specific primers and one pair of coelonema specific primers. The identification method comprises the following steps: obtaining a sample to be identified, taking genomic DNA of the material as a template, and using two specific primer pairs for amplification; performing agarose gel electrophoresis operation on the amplified product; and judging the authenticity of the interspecific hybrid by observing the electrophoresis strip.

The application belongs to the technical field of molecular biology, and particularly relates to a molecular marker primer group for identifying Huperzia serrata and H. phlegmaria and application thereof. The application provides a cpSSR primer group for identifying Huperzia serrata and H. phlegmaria, wherein the cpSSR marker primer group comprises one or more of Hup3, Hup15, Hup22 or Hup26. The chloroplast SSR marker primer can be used to accurately identify Huperzia serrata and H. phlegmaria. The method has the advantages of simple operation, low cost, high repeatability and the like, and can be widely applied to identification of true and false traditional Chinese medicinal materials, investigation of species resources and molecular breeding research.

This invention discloses a method for rapidly detecting conventional and hybrid varieties of industrial chili peppers and its application, relating to the field of molecular breeding technology. The method includes: extracting DNA from fresh leaves of the industrial chili pepper to be tested and performing whole-genome resequencing at a sequencing depth of 8×–12×; aligning the sequencing data to the chili pepper reference genome (Capsicum chinense cv. 'PBC932') to screen for high-quality heterozygous SNP loci; using a 1 Mb sliding window to statistically analyze the distribution density of heterozygous SNPs across the entire genome; and determining the variety type based on density thresholds: an average density <300 SNPs / Mb indicates a conventional variety, and >2000 SNPs / Mb indicates a hybrid variety. This invention eliminates the need for phenotypic observation in subsequent generations, requiring only leaves from current-generation plants to rapidly and accurately distinguish conventional and hybrid varieties at the molecular level. It features a short detection cycle, objective and reliable results, and is not limited by the number of molecular markers, providing strong technical support for efficient identification and early screening of industrial chili pepper germplasm resources.

This invention relates to the field of biotechnology, specifically to... StNCED3 The application of genes in improving the abiotic resistance of potatoes: This invention involves constructing... StNCED3 The screening and identification of gene overexpression vectors and transgenic lines, and their application in improving potato resistance to drought and low-temperature stress, revealed the overexpression of... StNCED3 The gene can significantly enhance the drought and cold resistance of potato plants, providing core functional genes and high-quality breeding materials for molecular breeding of potatoes for drought and cold resistance. It effectively solves the technical bottlenecks of weak drought and cold resistance of the current main potato varieties and unstable yield in arid and low-temperature production areas, and has important theoretical research value and industrial application prospects.