231 results about "Genetic stock" patented technology

The present application relates to the field of agricultural biotechnology, and specifically discloses a kind of corn ear roughness related molecular marker and its application.The present application provides the application of corn ear roughness molecular marker in ear roughness corn molecular marker assisted breeding, the molecular marker is Zm00001d020000 gene, the nucleotide sequence of the Zm00001d020000 gene is as shown in SEQ ID NO:1, the expression amount of the gene is positively correlated with the corn ear roughness trait, multiple SNP sites related to ear roughness are screened out by GWAS analysis, and simultaneously, QTL point analysis is used to be commonly positioned to SNP_84054216 located on the 7th chromosome, and the marker gene Zm00001d020000 for regulating ear roughness is excavated from the site, and the results of the present application provide technical support for molecular marker breeding of rough ear corn varieties.

The application provides a kind of construction and application for sesbania sesban DNA fingerprint, belong to sesbania molecular marker technical field, the application is based on sesbania genome sequencing, first develops and screens out 8 pairs of SSR primers with sequence specificity and rich polymorphism, by analyzing the agarose gel electrophoresis result of PCR amplification product of SSR primer in different varieties, the assignment of different size, type of PCR amplification product is carried out, to form the DNA fingerprint of each sesbania, the application has the characteristics of easy operation, good repeatability, simple data processing, low cost and the like, and lays a foundation for the classification, identification and utilization of sesbania germplasm resources.

The application discloses a SNP site and KASP marker combination related to contents and proportions of ginsenosides Rg1 and Rb1 in Panax notoginseng and application thereof, and belongs to the technical field of medicinal plant molecular breeding and quality evaluation. In view of the problems of lack of molecular markers synchronously related to contents and proportions of ginsenosides Rg1 and Rb1 in Panax notoginseng and long breeding cycle in the prior art, nine SNP site combinations located on a Chr11 chromosome of Panax notoginseng genome are screened, and a KASP marker combination and primer combination are developed based on four sites of SNP3-SNP6 among them. The KASP marker combination has strong specificity, is convenient to detect, has high throughput, is low in cost, and can accurately identify the contents and proportions of Rg1 and Rb1 in Panax notoginseng samples. The application can be used for Panax notoginseng germplasm resource screening and molecular marker assisted breeding, early identification of target traits is realized, the breeding cycle is shortened, the breeding efficiency is improved, and technical support is provided for directional cultivation of Panax notoginseng high-quality varieties.

This invention discloses the application of knocking down or knocking out the OsABA8ox2 protein or its encoding gene in improving the panicle germination resistance of rice seeds, belonging to the fields of biotechnology and plant genetic engineering technology. OsABA8ox2 The amino acid sequence of the protein is shown in SEQ ID NO.2, and the nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention utilizes CRISPR / Cas9 technology to directionally edit the target site sequence in this gene, as shown in SEQ ID NO.3, successfully obtaining rice nucleotide sequences as shown in SEQ ID NO.8, SEQ ID NO.9, and SEQ ID NO.10. OsABA8ox2 The mutant, to a certain extent, enhances the dormancy of rice seeds, effectively reducing the germination rate of rice panicles. The gene resources and gene editing technology provided by this invention offer an important technical approach for rapidly improving or creating rice varieties with significantly enhanced panicle germination resistance in agricultural production, demonstrating significant application value in production practice.

The present application relates to the technical field of molecular markers, and particularly relates to a SNP molecular marker of 'Zhongxiu 15' Carya illinoinensis and application thereof. The SNP molecular marker can be used to identify 'Zhongxiu 15' Carya illinoinensis, and is of great significance to germplasm resource identification of Carya illinoinensis and molecular marker breeding.

The application discloses a kind of based on molecular marker clustering analysis's cotton hybrid breeding combination screening method, the screening method includes: step S1: using multi-traits related molecular marker to the gene typing experiment of test cotton variety, data collection and arrangement are carried out;Step S2: excellent allele frequency calculation and feature matrix construction;Step S3: data standardization and principal component analysis dimension reduction;Step S4: optimal cluster number selection and genetic clustering;Step S5: each population character analysis;Step S6: heterosis potential score and combination screening.The technical problem that present cotton breeding parent selection lacks multi-traits comprehensive quantitative evaluation is solved, and the data of breeding decision-making and intelligentization are realized.

According to the disclosure, there is provided seed and plants of the hybrid corn variety designated CH010542. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CH010542, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010542 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to genetic complements of plants of variety CH010542.

This invention discloses a method and system for classifying pear varieties based on a minimum feature set, belonging to the field of agricultural product classification technology. The method involves acquiring raw physicochemical index data of pear samples, standardizing the data to obtain a standardized dataset, and then performing principal component analysis (PCA) to verify the separability of the raw physicochemical index data. An orthogonal partial least squares discriminant analysis (OPLS-DA) model is used for pairwise comparisons across multiple groups. After screening and ranking, a subset of key difference components is obtained. This subset is then ranked by importance and sequentially input into a random forest model for training. After cross-validation, a minimum feature set is obtained, and the final random forest model is derived based on this minimum feature set for classifying pear varieties. This invention ensures the reliability of national standard testing methods while effectively reducing the number of testing indicators, achieving high-efficiency and low-cost accurate identification of agricultural products, effectively reducing testing costs, and promoting industry development.

The present application belongs to the technical field of agricultural machinery, and particularly relates to a low-loss harvester capable of realizing whole-plant uprooting of low-height soybean varieties and real-time recovery of mature soybean pod-seed grains in a plot breeding scene. The present application, in view of the characteristics of mature soybean being prone to pod-shattering, realizes synchronous recovery of falling grains in the conveying process by setting a trapezoidal groove, a baffle and a flexible bellows, thereby minimizing the harvesting loss. Understandably, when the soybean plants are shaken by the machine, the grains fall downward to the trapezoidal groove, which is inclined, and the falling grains slide to the bottom under the action of gravity. In addition, the plot breeding low-height soybean special low-loss harvester of the present application has good adjustment flexibility and operation convenience, and the uprooting height, angle, belt gap and tension can be accurately adjusted, and it has strong adaptability; the movable baffle has the functions of protection and unloading slope, thereby reducing the labor force for field sampling.

The present application relates to the field of plant genetic engineering technology, in particular to the application of ZmAAAP59 protein and its coding gene in regulating corn tolerance to high temperature stress, by knocking out ZmAAAP59 gene in corn, it is determined that the mutant has obvious high temperature tolerance phenotype, it is verified that corn ZmAAAP59 gene can negatively regulate plant heat resistance, by reducing the expression amount of ZmAAAP59 gene in plants, the heat resistance of plants can be effectively increased.The discovery of the heat resistance function of ZmAAAP59 gene provides a new gene target and resource for breeding heat-resistant plant varieties, has important significance for the study of the heat tolerance molecular mechanism of corn, and lays a certain theoretical foundation for the study of the mechanism of plant response to high temperature stress and the molecular mechanism of resisting adverse environment.

This invention provides SSR molecular markers associated with the yield trait of Corydalis yanhusuo, belonging to the field of molecular genetics. This invention utilizes 24 pairs of SSR markers to perform regression analysis on the genotype and yield trait of individual Corydalis yanhusuo individuals. Using the GLM program, association loci for the yield trait of Corydalis yanhusuo were detected. The SSR molecular markers CYC2-4 and CYC8-10 were found to be highly significantly associated, explaining 16.72% and 14.06% of the phenotypic variation, respectively. The primers provided by this invention for amplifying the aforementioned SSR molecular markers can be used to screen for high-yielding Corydalis yanhusuo germplasm, locate key genes controlling yield traits, and for marker-assisted breeding, which is of great significance for cultivating high-yielding Corydalis yanhusuo varieties.

The application discloses a nuclear factor YB subunit gene GhNF-YB3 and application thereof, the nucleotide sequence of the gene GhNF-YB3 is shown as SEQ ID NO. 1; through yield character investigation on 245 upland cotton varieties, GWAS and eQTL positioning are carried out in combination with population genome resequencing and ovule transcriptome sequencing one day after flowering, and a site gene GhNF-YB3 significantly related to cotton yield character is obtained. The gene expression of the gene GhNF-YB3 is significantly positively correlated with two yield characters of seed index and boll weight, and the gene may be a causal gene for regulating cotton seed index character. The nuclear factor YB subunit gene GhNF-YB3 of the application can be applied to identification of high-yield upland cotton varieties and improvement of cotton yield character.

The application belongs to the technical field of green plant protection cultivation, and discloses a standardized planting and management method for pollution-free green agricultural products, which realizes multi-index screening of soil heavy metals, pesticide residues, microorganisms and water, soil and air, establishes a soil plant protection database, and applies passivation materials, compound beneficial microorganism groups and decomposed organic fertilizers to the soil according to pollution, continuous cropping and short soil fertility, and improves the stress resistance of seedlings by matching seed pretreatment; the disease and pest risk grades of plots are divided by weighting and quantifying five types of planting data, and a risk map is drawn, high, medium and low risk plots are differentially configured with pest-resistant varieties, intercropping, crop rotation and pest-repellent crops, and a hierarchical prepared prevention and control mechanism is matched, the planting environment is optimized from the aspects of farmland basement and field ecological layout; through the fusion of the Internet of Things, unmanned aerial vehicle remote sensing, pest trapping and meteorological data, the early latent characteristics of diseases and pests are captured through a deep learning model, and the development trend of diseases and pests is deduced in advance and divided into three levels of early warning.

This invention belongs to the field of hybrid identification technology of *Fraxinus pubescens*, specifically relating to an InDel marker and its application for identifying the authenticity of hybrids between *Fraxinus pubescens* and *Astragalus membranaceus*. The InDel marker described in this invention is located at positions 187 to 240 of the sequence shown in SEQ ID NO:1, and the sequence of the InDel marker is shown in SEQ ID NO:2. Furthermore, based on this InDel marker, this invention has obtained a set of primers with stable polymorphism, high amplification efficiency, and clear bands, establishing a technical system that can identify the authenticity of hybrid offspring at the seedling stage without relying on expensive equipment. This provides an efficient and low-cost molecular tool for the breeding of new varieties among *Fraxinus pubescens* species, the protection of variety rights, and market supervision, and has significant application value.

The application discloses a high-throughput KASP molecular marker significantly related to plant height of Gossypium hirsutum and application, and belongs to the technical field of cotton molecular breeding. The KASP marker is successfully developed based on cotton plant type related genes and resequencing data of germplasm resources. Through genotyping and phenotype correlation analysis in 246 natural Gossypium hirsutum populations, four KASP markers (PH-TK1-PH-TK4) significantly related to plant height in three environments are screened. The regulation effect and aggregation effect of the markers on plant height are verified in a separation population. Experiments prove that the KASP marker can be used for high-throughput and accurate genotyping of plant height in early cotton breeding, and provides an effective tool for molecular marker assisted selection of cotton ideal plant type, and helps to accelerate the breeding process of cotton varieties suitable for mechanical harvesting.

The application provides a gene for improving nitrogen utilization efficiency of wheat under nitrogen deficiency conditions and application thereof, and belongs to the technical field of biological agriculture. The gene erf related to the nitrogen deficiency tolerance trait is obtained through normal nitrogen and nitrogen deficiency stress treatment of natural wheat varieties in the world and whole genome association analysis and verification. The erf gene not only responds to the up-regulated expression under the nitrogen deficiency stress of wheat, but also promotes the growth and nitrogen accumulation of wheat under the nitrogen deficiency conditions through a negative adjustment mode, so as to improve the nitrogen utilization efficiency of wheat. The erf gene provided by the application can effectively regulate the growth and nitrogen utilization of wheat under the nitrogen deficiency conditions, provides a new strategy for the construction and breeding of wheat varieties with nitrogen deficiency tolerance, and provides an effective site for the detection and variety identification of the wheat environment under the nitrogen deficiency.

The TaSL1 protein or biological material provided by this invention has the function of increasing the number of grains per ear and the length of the ear in wheat. After reducing the expression of TaSL1 protein in wheat, the transgenic wheat obtained has a significantly increased number of grains per ear, ear length, and awn length compared with the control. The TaSL1 protein or biological material provides a new genetic resource for breeding wheat varieties with high grain count and long ears, which is of great significance for improving wheat yield.

The application discloses a SNP molecular marker related to the content of oleic acid in sesame seeds and application, and belongs to the technical field of molecular marker breeding. The SNP molecular marker related to the content of oleic acid in sesame seeds is located at 2754308 bp of the No. 3 chromosome, the genotype GG corresponds to the content of oleic acid which is significantly higher than that of AA (the difference reaches the extremely significant level of 0.01), and the accurate differentiation of high / low oleic acid germplasm can be realized. Early genotype identification can be carried out at the seedling stage of sesame by using the marker, and the breeding cycle is greatly shortened and the selection efficiency is improved without waiting for seed maturation. The marker is stable and reliable under different genetic backgrounds, is less affected by the environment, and is simple, high-flux and low-cost in detection, is suitable for large-scale automatic screening, can be directly used for molecular marker assisted selection of high-oleic-acid sesame varieties, accelerates the cultivation of high-quality new varieties, and has important application value for improving the sesame oil quality and market competitiveness.

This invention provides the application of the GbWAK5 gene in regulating the salt tolerance of sea island cotton, belonging to the field of plant genetic engineering technology. The nucleotide sequence of the GbWAK5 gene described in this invention is shown in SEQ ID NO.1, and the amino acid sequence of the protein encoded by the GbWAK5 gene is shown in SEQ ID NO.2. VIGS experiments have demonstrated that the GbWAK5 gene participates in the salt tolerance of sea island cotton by regulating ion homeostasis, and sea island cotton containing or highly expressing the GbWAK5 gene exhibits better salt tolerance. These results provide important information for understanding the members of the sea island cotton WAK gene family and provide potential target genes for further breeding of salt-tolerant varieties.

This invention provides a rice gene Bph54 for resistance to brown planthopper and its application. The rice provided by this invention... Bph54 Genes are of great significance for breeding new rice varieties resistant to brown planthoppers. This invention successfully cloned the rice Bph54 gene using map-based cloning, and performed functional annotation and identification. This gene exhibits complete dominance, and both homozygous and heterozygous genotypes confer equal resistance to brown planthoppers in rice. This characteristic is beneficial for the application of this gene in breeding. Based on this, the Bph54 gene can be introduced into brown planthopper-susceptible plant varieties through traditional breeding techniques such as hybridization and backcrossing, or through genetic engineering, to cultivate new varieties of plants resistant to brown planthoppers with stable genetic traits. This invention provides important germplasm resources for effectively reducing insect damage and achieving increased and stable crop yields.

This invention discloses an SSR molecular marker primer set for identifying Amomum villosum varieties, along with its identification method and applications. The SSR molecular marker primer set provided by this invention contains 27 primer pairs and possesses advantages such as high polymorphism, stable results, good reproducibility, and simple operation. Using this primer set for amplification and detection can be used to identify different Amomum villosum varieties, construct a DNA fingerprint information database of Amomum villosum germplasm, and compile a statistical database of genetic diversity information. This invention also provides a method for identifying Amomum villosum varieties based on SSR molecular markers. By amplifying Amomum villosum samples using the SSR molecular marker primer set and performing statistical analysis based on the number of differential loci, different Amomum villosum varieties can be quickly and accurately distinguished and identified, clarifying the genetic background of Amomum villosum germplasm, guiding scientific breeding, and providing a more effective method for identifying Amomum villosum varieties. This method is of great significance for Amomum villosum breeding.

This invention discloses the application of the OsSR1IP1 gene in regulating tillering angle in rice. It belongs to the field of plant genetic engineering technology. This invention uses genetic engineering technology to obtain transgenic rice that stably overexpresses OsSR1IP1. By comparing the growth of wild-type and OsSR1IP1-overexpressing transgenic rice, it was found that the tillering angle of OsSR1IP1-overexpressing transgenic rice plants was significantly increased. Therefore, this invention successfully identified a key gene regulating rice plant architecture, which has theoretical significance and application value for enhancing the lodging resistance and increasing yield of rice varieties, and is of great importance to agricultural development and national food security.

The present application relates to the technical field of plant breeding, and particularly relates to a molecular marker and application thereof in breeding soybean varieties with high oil content and high protein content. The molecular marker is constructed based on a SNP site; based on the genome version number Wm82.a2.v1, the SNP site is located at the 8th chromosome of soybean at the 9020004th position, and the polymorphism is T / A. The application comprises: (1) predicting or detecting the oil content or protein content of soybean; (2) identifying or breeding soybean varieties with high oil content or protein content; (3) molecular marker assisted breeding of soybean; (4) improvement of soybean varieties related to oil content or protein content; (5) improvement of soybean germplasm resources. The molecular marker provided by the present application has the characteristics of double trait synergistic correlation, strong specificity, efficient detection process and the like, and can be widely applied to the scenes of molecular marker assisted breeding of soybean, rapid evaluation of germplasm resources and the like, and provides key technical support for breeding of soybean varieties with double optimal traits.

This invention belongs to the field of genetic engineering technology and discloses... TaRNS4 Application of genes in wheat resistance to stripe rust. TaRNS4 The nucleotide sequence of the gene is shown in SEQ.ID.NO:1. This gene is involved in wheat resistance to stripe rust and plays a positive regulatory role in wheat resistance to stripe rust. Overexpression of the gene... TaRNS4 Genes enhance wheat's resistance to stripe rust. Overexpression TaRNS4 The gene can activate the wheat MAPK signaling pathway, increase the expression of disease resistance-related genes in wheat, thereby improving wheat's resistance to stripe rust. Overexpression TaRNS4 The gene can also reduce yield loss in wheat caused by stripe rust. This invention provides a germplasm resource for the green control of wheat stripe rust and offers new ideas for breeding rust-resistant wheat varieties.

This invention discloses a method for rapidly detecting conventional and hybrid varieties of industrial chili peppers and its application, relating to the field of molecular breeding technology. The method includes: extracting DNA from fresh leaves of the industrial chili pepper to be tested and performing whole-genome resequencing at a sequencing depth of 8×–12×; aligning the sequencing data to the chili pepper reference genome (Capsicum chinense cv. 'PBC932') to screen for high-quality heterozygous SNP loci; using a 1 Mb sliding window to statistically analyze the distribution density of heterozygous SNPs across the entire genome; and determining the variety type based on density thresholds: an average density <300 SNPs / Mb indicates a conventional variety, and >2000 SNPs / Mb indicates a hybrid variety. This invention eliminates the need for phenotypic observation in subsequent generations, requiring only leaves from current-generation plants to rapidly and accurately distinguish conventional and hybrid varieties at the molecular level. It features a short detection cycle, objective and reliable results, and is not limited by the number of molecular markers, providing strong technical support for efficient identification and early screening of industrial chili pepper germplasm resources.

This invention relates to the field of biotechnology and discloses a method for regulating the angle of fruiting branches in upland cotton. GhPDF1 Genes and their applications, the aforementioned GhPDF1 The coding region sequence of the gene is shown in SEQ ID NO:1; GhPDF1 The gene ID is GH_D04G0386.1 ;A sort of GhPDF1 The application of genes in regulating the fruiting branch angle of upland cotton, either for regulating the fruiting branch angle of upland cotton or for breeding upland cotton varieties with a compact plant type, is described. GhPDF1 Genes regulate the fruiting branch angle of upland cotton by modulating their expression levels in upland cotton cells. This invention provides a precise genetic modification pathway, demonstrating the ability to inhibit [the growth of] upland cotton fruiting branches using VIGS technology. GhPDF1 Gene expression can significantly increase the angle between cotton fruiting branches, making the plant type looser, providing a clear gene target and effective technical means for using genetic engineering technology to cultivate new cotton varieties with compact plant type.

This invention relates to the field of biotechnology, specifically to... StNCED3 The application of genes in improving the abiotic resistance of potatoes: This invention involves constructing... StNCED3 The screening and identification of gene overexpression vectors and transgenic lines, and their application in improving potato resistance to drought and low-temperature stress, revealed the overexpression of... StNCED3 The gene can significantly enhance the drought and cold resistance of potato plants, providing core functional genes and high-quality breeding materials for molecular breeding of potatoes for drought and cold resistance. It effectively solves the technical bottlenecks of weak drought and cold resistance of the current main potato varieties and unstable yield in arid and low-temperature production areas, and has important theoretical research value and industrial application prospects.

The application belongs to the technical field of cotton molecular breeding, and particularly relates to a KASP molecular marker associated with the flowering trait of upland cotton and an application thereof. The nucleotide sequence of the molecular marker is shown as SEQ ID NO. 27. The molecular marker contains a SNP site. The SNP site is located at the 14th bp from the 5' end of the sequence shown as SEQ ID NO. 27. The polymorphism is G / A. The A allele is a favorable allele for promoting early flowering. The application also develops a primer set for detecting the molecular marker. The early flowering germplasm resource of upland cotton can be screened, the flowering time of upland cotton can be predicted, or early flowering varieties of upland cotton can be assistedly selected by using the primer set. The application also develops a method for assisting in the selection of early flowering varieties of upland cotton. The method can shorten the breeding cycle of the flowering trait of cotton and improve the breeding efficiency of the early flowering trait.

This invention discloses a photoperiod regulation gene of foxtail grass. SvEhd1 Its application in shortening the research cycle of rice resistance to rice blast falls under the fields of genetic engineering and plant breeding. SvEhd1 The gene nucleotide sequence is shown in SEQ ID NO.1. This invention utilizes cloning... SvEhd1 Genes and their promoters were used to construct recombinant expression vectors, which were then transformed into the blast-susceptible rice variety "Taipei 309," resulting in transgenic rice materials with significantly shortened growth periods. Under natural and artificial long-day conditions, the heading time of the transgenic plants was significantly earlier than that of the wild type, the generation rate was accelerated, and the blast susceptibility of the original variety was not altered. The transgenic materials and methods provided by this invention can effectively shorten the experimental cycle for verifying the function of rice blast resistance genes and evaluating resistance, providing an efficient experimental system for rice disease resistance research.

This invention relates to the field of variety breeding and identification technology in molecular biology, specifically a molecular marker, primers, kit, and application for identifying the rubber tree variety 'Yunyan 272'. The molecular marker is located at bases 10110474–10110618 on chromosome CM021232_1, and its nucleotide sequence is shown in SEQ ID No. 1. This invention provides molecular marker primers and a kit for the rubber tree variety 'Yunyan 272'. This technology can specifically identify and amplify the DNA sequence of the 'Yunyan 272' variety, enabling accurate identification. This helps improve the accuracy of variety identification, optimize variety selection and regional suitability assessment, thereby providing a scientific basis for rubber tree planting and breeding, and promoting the healthy development of the rubber industry. The molecular marker, primer pairs, or kit of this invention are easy to operate and suitable for large-scale application.