1440 results about "Genetic stock" patented technology

The invention discloses a cowpea 5K SNP (Single Nucleotide Polymorphism) liquid chip and application thereof in variety identification, and belongs to the technical field of biology. The liquid chip provided by the invention comprises a set of nucleotide probes for detecting 5847 SNP sites of cowpea, and can be widely applied to different scenes of germplasm resource identification, genetic diversity evaluation, variety identification, genetic relationship identification, purification and rejuvenation of local varieties and the like of different cowpea varieties; an effective scientific basis is provided for cowpea variety identification, the variety identification and evaluation efficiency is improved, and the method has a remarkable application value.

The invention discloses a molecular marker closely linked with wheat flag leaf width major QTL and application of the molecular marker in prediction of wheat flag leaf width, and belongs to the technical field of wheat molecular biotechnology and breeding. Wherein the molecular marker is 4BFLW-290, the nucleotide sequences of the molecular marker are as shown in SEQ ID NO: 3 and SEQ ID NO: 4, the specific primer pair of the molecular marker is 4BFLW-290-F / R, and the nucleotide sequences of the specific primer pair are as shown in SEQ ID NO: 1 and SEQ ID NO: 2. The molecular marker 4BFLW-290 provided by the invention fully embodies the flag leaf width major QTL of the wheat variety (line), can quickly and accurately predict whether the wheat variety (line) has the flag leaf width major QTL or not, and provides excellent gene resources and selection tools for wheat yield trait molecular breeding.

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to oil content and protein content of soybean and application of the molecular marker. The molecular marker is constructed on the basis of SNP sites; based on a genome version number Wm82. A2. V1, the SNP site is located at the 8605814th site of the No.8 chromosome of the soybean, and the polymorphism is A / G. The application comprises the following steps: (1) predicting or detecting the oil content or protein content of soybeans; (2) identifying or cultivating soybean varieties with high oil content or protein content; (3) soybean molecular marker assisted breeding; (4) soybean variety improvement related to oil content or protein content; and (5) soybean germplasm resource improvement. The molecular marker related to the soybean oil content and the protein content is obtained through research and screening, and the molecular marker can be applied to cultivation of soybean varieties with high oil content and high protein content and has important value in the field of soybean breeding.

The invention relates to the technical field of agricultural informatization and intelligent decision making, and discloses a vegetable growth cycle pest control decision making system and method based on a knowledge graph. According to the method, an unstructured agricultural data stream including vegetable varieties, growth stages, environmental parameters, pest and disease records and geographic information is collected from an original database. A data tuple set with a time stamp is generated by performing scanning identification and content extraction on a data stream. Then, clustering is carried out according to the growth stages to which the tuples belong, and stage feature clusters sorted according to the growth cycle are formed; and performing multi-dimensional feature correlation analysis on the stage feature cluster, and establishing a dynamic coupling relationship among the environmental parameters, the disease and pest history and the geographic space features under the growth cycle dimension. And based on the dynamic coupling relationship, generating disease and pest control decision parameters of the vegetables in the specific planting area in each growth cycle stage. According to the invention, accurate matching between the control decision and the crop physiological time sequence and regional characteristics is realized.

The invention relates to a product of an SNP (Single Nucleotide Polymorphism) molecular marker related to sow litter size character detection and application of the product. The SNP molecular marker comprises Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt The nucleotide sequence of the gene is shown as SEQ ID No.1, and the nucleotide sequence of the gene is shown as SEQ ID No.1. The product at least comprises an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is as shown in SEQ ID No.2, and the nucleotide sequence of the downstream primer is as shown in SEQ ID No.3. The SNP molecular marker provided by the invention can be used for breeding, breeding and evaluating multi-variety sows with high reproductive performance.

The invention relates to a mulberry MaBEH1 / 2 gene for improving plant salt tolerance and application thereof, and belongs to the technical field of plant genetic engineering and biology. Wherein the coding nucleotide sequence of the mulberry MaBEH1 / 2 gene for regulating and controlling the salt tolerance of the woody plant is shown as a sequence 3; and the amino acid sequence of the MaBEH1 / 2 expression protein is shown as a sequence 4. According to the invention, MaBEH1 / 2 is transferred into 84K poplars to obtain MaBEH1 / 2-OE transgenic poplars; compared with a non-transgenic poplar, the salt stress tolerance of the MaBEH1 / 2-OE transgenic poplar can be remarkably enhanced. The invention provides key gene resources and technical support for genetic improvement of salt-tolerant forest varieties.

The application provides a wheat flour wet gluten content main effect site and SNP marker and application, and belongs to the wheat breeding technical field, and mainly provides SNP molecular markers Marker45889, Marker45896, Marker45348, Marker45403, Marker45397 and Marker45431, and the SNP site provided by the application has important significance for detecting and breeding wheat flour high FWG varieties, lines and breeding materials, and molecular markers can be developed according to the SNP site, so that the wheat quality breeding efficiency is accelerated.

The invention discloses a molecular marker related to thousand seed weight of wheat, a detection primer and application of the molecular marker and the detection primer, and belongs to the technical field of molecular marker-assisted breeding. The nucleotide sequence of the molecular marker TaDTX50-STS is as shown in SEQ ID NO. 1; basic groups from the 502 site to the 661 site of the sequence as shown in SEQ ID NO. 1 have insertion / deletion mutation. The InDel molecular marker related to the thousand seed weight character in a wheat genome is identified and named as TaDTX50-STS, and by detecting the polymorphism or genotype of the InDel molecular marker, the InDel molecular marker can be used for identifying or assisting in identifying the thousand seed weight character of wheat, assisting in breeding dominant varieties with high thousand seed weight of wheat and improving the wheat breeding efficiency.

The present invention belongs to the field of biotechnology, and specifically relates to a GmSGT2 gene related to plant height and branch development, and a mutant thereof and the use thereof. A Glycine max mutant having fewer branches and decreased plant height is obtained by means of EMS mutagenesis, and a target gene thereof that is located by means of a map-based cloning technique is a GmSGT2 gene of Hedou 12. It is found through searching that the gene encodes soyasapogenol B glucuronide galactosyltransferase, and can galactosylate soyasapogenol B monoglucuronide, thereby affecting the plant height of Glycine max and reducing the branches of Glycine max. Therefore, the GmSGT2 mutant can be used for cultivating dwarf high-yield Glycine max varieties, and is of great significance in the breeding of ideal plant types of Glycine max and research on important agronomic traits of plants. The mutant has broad application prospects and great research value with regard to understanding the plant height and branch regulation mechanism of Glycine max and improving the process of Glycine max breeding; and can provide an excellent germplasm resource stock for regulating the close planting and high-yield breeding of Glycine max.

The invention discloses an unmanned aerial vehicle LiDAR-based high-canopy-density crop plant height double-end adaptive inversion method and system, and relates to the technical field of agricultural remote sensing. The method comprises the following steps: acquiring a crop canopy point cloud and extracting density, skewness and original ground elevation characteristics; constructing a near-surface pseudo ground layer thickness correction model, introducing a pseudo ground layer thickness parameter to carry out pressing correction of a physical layer on an original ground elevation, and reconstructing a real bare soil layer ground reference; constructing a canopy end density-form adaptive model, and dynamically calculating the optimal upper boundary percentile by using the point cloud density and the elevation distribution skewness; and calculating the final plant height based on a double-end correction result. According to the method, the problems of systematic overestimation caused by near-surface pseudo ground blocking and poor adaptability of a fixed threshold value to the canopy form in LiDAR ground detection under the high-canopy-density canopy are effectively solved, and high-precision and robust plant height automatic extraction under multiple varieties and different flight heights is realized under the condition that external terrain auxiliary data is not needed.

The invention discloses a rice drought resistance phenotype detection and genetic analysis method based on a phenotypic robot, which is characterized in that rice hyperspectral phenotypic character data is acquired based on the phenotypic robot, and the relative water content RWC of rice is predicted. Through high-frequency data acquisition, RWC (t) based on a time sequence is obtained, RWC (t) curve change characteristic indexes are calculated, the dynamic change process of a rice experiment material in the whole drought experiment period is quantified, the drought resistance index DTI of the rice experiment material is obtained, and the drought resistance of the rice variety is comprehensively evaluated. DTI as a user-defined phenotypic character can be used for whole genome association analysis (GWAS), and a key gene for controlling the drought resistance of rice is excavated. According to the invention, the problems of low efficiency and low flux of traditional rice phenotype detection are solved, phenotype-gene mining based on the mobile robot is realized, and a new way is expanded for improvement of rice genetic breeding.

The invention discloses an application of a BnaMYB7 gene or an encoded protein thereof in regulating and controlling the oil content of rape, and belongs to the fields of genetic engineering and crop genetic breeding. The nucleotide sequence of the BnaMYB7 gene is shown as SEQ ID NO: 1 or SEQ ID NO: 2, an agrobacterium-mediated genetic transformation method is used, an overexpression vector of the BnaMYB7 gene and a CRISPR / Cas9 gene editing vector are respectively transformed into a genome of the rape, the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is obtained, and the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is determined by measuring the oil content of seeds of overexpression and mutant materials. It is found that the BnaMYB7 gene positively regulates the oil content of the rape, so that rape varieties with different oil contents can be obtained by using the gene to perform molecular breeding on the rape. The feed additive is of great significance in relieving grain and oil tension and guaranteeing feed safety.

The invention provides a closely linked molecular marker primer of a rice brown planthopper resistant major gene Bph56 as well as a marking method and application of the closely linked molecular marker primer. According to the invention, genotypes of F2 individual plants obtained by hybridizing a rice insect-resistant variety C127 (or) and KW (or) are combined with brown planthopper resistance levels of an F3 family for genetic linkage analysis, a resistance major gene Bph56 carried by the insect-resistant variety C127 is identified, and a region of the resistance major gene Bph56 is reduced to a 77kb fragment limited by markers 6M26.278 and 6M26.355 on two sides and is closely linked with a molecular marker 6M26.355. The molecular marker 6M26.355 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C127 and derived varieties (lines) of the insect-resistant variety C127 contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper-resistant character is greatly improved, and the brown planthopper-resistant rice variety (line) containing Bph56 is obtained.

The invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker closely linked with soybean plant height QTL qPH1.01 and application of the molecular marker. According to the present invention, the major QTL qPH1.01 for regulating corn plant height variation is identified by using linkage analysis, the significantly associated SNP is located at the 27th, 318th and 904th bases of the first chromosome of the reference genome Glycinemaxv2.1, 6.28-11.14% phenotypic variation can be explained in the single environment, the PARMS marker developed by using the SNP is used to detect 96 soybean varieties, the typing is clear, the operation is simple and convenient, the method is suitable for soybean plant polymer breeding, and the application prospect is broad. And a new high-yield soybean variety with proper plant height and lodging resistance is cultivated.

The invention discloses a molecular marker closely linked with a cowpea salt tolerance major QTL (quantitative trait loci) and a primer sequence of the molecular marker. The name of the cowpea salt tolerance major QTL is SC300126, a KASP type molecular marker 300126 closely linked with the cowpea salt tolerance major QTL is located at the position of 43816166 bp on a chromosome 11, the marker can be used for amplifying a material to be detected, and whether a sample carries key allelic variation for controlling salt tolerance or not is judged according to the genotype of the marker. The molecular marker and the primer provided by the invention can be used for quickly screening the cowpea salt tolerance major QTL, and compared with a traditional phenotype identification method, the molecular marker and the primer have the advantages of high detection flux, accurate and reliable result and the like, and the typing accuracy can reach 99% or above. The technology can significantly improve the breeding efficiency of the salt-tolerant variety of cowpea and shorten the breeding period.

The invention discloses an oat KASP marker, a fingerprint spectrum and application, and relates to the technical field of molecular breeding. The KASP marker comprises 96 SNP (Single Nucleotide Polymorphism) sites, and the position information and the polymorphism of the KASP marker are shown in a table 1. The invention also provides a gene chip for analyzing the KASP marker and an oat variety characteristic fingerprint spectrum constructed by using the KASP marker, and the KASP marker, the gene chip and the oat variety characteristic fingerprint spectrum provided by the invention can be used for screening, identifying and tracing oat quality. The method can be used for identifying the authenticity of the seeds and the genetic relationship between the parent and offspring of the oat, and has important significance for protecting and improving the germplasm resources of the oat.

The invention relates to a method and primers for analyzing genetic diversity of nardostachyos root and rhizome based on ISSR molecular markers, and belongs to the technical field of molecular biology. The first technical problem solved by the invention is to provide an ISSR molecular marker primer for genetic diversity analysis of rhizoma nardostachyos. The sequences of the primers comprise UBC809, UBC823, UBC840, UBC842, UBC847 and UBC850, and the UBC840, the UBC842, the UBC847 and the UBC850 are The six ISSR primer sequences provided by the invention are stable in PCR amplification reaction in rhizoma nardostachyos, clear in amplified fragment and relatively good in stability and repeatability. The method for analyzing the genetic diversity of the rhizoma nardostachyos based on the ISSR molecular marker provides technical support for analysis of genetic structures and genetic relationships of different geographical populations and different characters of the rhizoma nardostachyos, can complete identification of test materials in a low-cost, large-batch and short-time manner, and provides a basis for protection of rhizoma nardostachyos resources and breeding of excellent varieties.

The invention discloses a group of molecular markers related to the oil yield of carya illinoensis and application of the molecular markers, and relates to the technical field of biology. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO. 1-2. The molecular marker disclosed by the invention can be used for marker assisted selection (MAS) and genome selection (GS) of the carya illinoensis, so that the genetic improvement progress of the variety of the carya illinoensis can be accelerated.

The invention relates to the technical field of biological genetic engineering, in particular to application of a brassica napus BnaA01G0331000ZS gene in drought stress resistance. The BnaA01G0331000ZS gene is cloned by using cDNA of double 11 leaves as a template through a sequence published in a brassica napus genome database, an over-expression vector is constructed and genetically transformed into rape variety high oil for transgenic function verification, the drought resistance of the obtained over-expression transgenic line is obviously enhanced compared with that of a wild type, and the BnaA01G0331000ZS gene has the advantages that the BnaA01G0331000ZS gene is obtained; therefore, the gene can positively regulate the drought stress resistance of the rape. Therefore, the invention provides a new gene resource for adversity stress resistance of rape.

The invention belongs to the technical field of agricultural intelligent decision support and crop cultivation optimization, and particularly provides a food crop precision cultivation decision method, system and device and a medium, and the method comprises the following steps: obtaining cultivation related data of all sites in a target area; downscaling the meteorological data through a bilinear interpolation method, and merging the meteorological data with the soil data and the management measure data into a grid data set; using a random forest model and a crop growth model to determine a plurality of key variety parameters having the maximum influence on the output and the growth period importance; a random forest model is adopted to construct a mapping relation between the key variety parameters and meteorological data and soil data in the grid data set, and the key variety parameters of each grid in the target area are obtained; and utilizing the random forest model and the crop growth model to obtain the sowing date and the planting density corresponding to the target yield of each grid. According to the method, the precision breakthrough on the decision-making scale is realized, and the simulation effect precision is improved by 20-30%.

This invention discloses the TaJDR1 gene and its application in improving plant resistance to Fusarium head blight and drought. The nucleotide sequence of the TaJDR1 gene is shown in SEQ ID NO.1, and its encoded amino acid sequence is shown in SEQ ID NO.2. This invention is the first to clone the TaJDR1 gene from the local wheat variety Wangshuibai and achieve overexpression of this gene in wheat using gene gun-mediated genetic transformation technology. The results show that the transgenic wheat exhibits significantly improved resistance to Fusarium head blight and drought resistance. Since the TaJDR1 gene originates from wheat itself, overexpression does not affect food safety; therefore, this invention can be widely applied in the field of disease and drought resistance breeding for various plants.

The invention discloses application of protein TaTDM and related biological materials thereof, and belongs to the field of plant breeding. The amino acid sequence of the protein TaTDM is as shown in SEQ ID NO: 2 or SEQ ID NO: 4 or SEQ ID NO: 6. The TaTDM gene knockout homozygous mutant strains tatdmm1 and tatdmm2 are obtained by carrying out gene knockout on wild type wheat Fielder, and it is found that at the temperature of 25 DEG C, the total floret number of each spike of the Fielder, the tatdmm1 and the tatdmm2 and the survival rate under the water shortage treatment condition are remarkably higher than those of the wild type wheat Fielder. The protein TaTDM has an important application value in regulation and control of wheat ear type characters and stress tolerance, provides a high-quality germplasm resource, and is of great significance in cultivation of drought-enduring plant varieties and improvement of crop yield.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker for auxiliary selection of the leaf number of brassica napus, a primer group, a detection method and application, and relates to the technical field of biology and rape breeding. The SNP marker is located at the 9283404 bp position of a brassica napus A02 chromosome, a basic group of the site is a T allele or a C allele, and the T allele is associated with a leafy character. The SNP marker provided by the invention can solve the technical problem that the breeding efficiency of the high-photosynthetic-efficiency ideal-plant-type rape variety is restricted due to the fact that an effective molecular marker closely linked with the leaf number character of the brassica napus is lacked in the prior art and the character cannot be quickly and accurately screened in the early breeding stage.

The invention provides a molecular marker composition related to rice seedling stage cold resistance, a primer composition, a kit and application thereof, and belongs to the technical field of crop genetic breeding. The invention provides a molecular marker composition related to rice seedling stage cold tolerance. The molecular marker composition comprises at least one of a first SNP marker, a second SNP marker, a third SNP marker, a fourth SNP marker, a fifth SNP marker and an InDel marker. The invention further provides a primer composition for detecting the cold resistance of the rice in the seedling stage. The primer composition comprises at least one of a first primer group, a second primer group, a third primer group, a fourth primer group, a fifth primer group and an InDel detection primer group. The molecular marker composition and the primer composition disclosed by the invention can be used for accurately distinguishing a cold-tolerant rice variety and a cold-sensitive rice variety in a seedling stage, and scientific guidance is provided for breeding and improvement of a new cold-tolerant rice variety.

The invention discloses a camellia chekiangoleosa improved variety breeding method and a camellia chekiangoleosa improved variety breeding system based on function superposition, and the method comprises the following steps: cloning excellent single plants through a high-stem grafting technology, and establishing a specific germplasm resource library; determining three types of phenotypic data of a floral leaf character set, an oil yield character set and an oil quality character set; the method comprises the following steps: presetting six types of application scenes such as an edible oil production raw material forest and a specific natural compound production raw material forest, and constructing a comprehensive evaluation model by adopting a hierarchical weight distribution method: distributing first-level weights for three types of character sets, and then distributing second-level weights for characters in subsets; standardizing the character of the floral leaf by adopting a binary comparison method, and standardizing the character of the oil yield and the character of the oil quality by adopting a centesimal system; and screening a target improved variety according to a comparison result of the comprehensive score of the to-be-evaluated variety and the average score of the existing improved variety. By means of the method, superposition breeding of ornamental, oil production and oil quality functions is achieved, and the adaptability of improved varieties and industrial scenes is improved.

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.

The invention belongs to the technical field of plant molecular breeding, and particularly relates to a KASP molecular marker related to the oil content of soybeans and application of the KASP molecular marker. The molecular marker is based on a soybean Glyma. 13G035200 gene, and targets an SNP (Single Nucleotide Polymorphism) site, which is remarkably associated with the soybean oil content, in the gene. The invention further provides a specific primer combination and a detection method of the molecular marker, soybean genotypes can be rapidly distinguished through PCR amplification and fluorescence signal detection, and high-oil-content soybean individuals can be accurately screened. The molecular marker has the advantages of being high in specificity, efficient in detection, reliable in result and the like, can be applied to soybean molecular marker assisted breeding, germplasm resource screening and high-oil soybean variety cultivation, remarkably shortens the breeding period, reduces the breeding cost, and provides an efficient tool for precise improvement of the soybean oil content character.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to a soybean branching number gene QNE1 and application thereof, and relates to the technical field of molecular genetics and crop genetic breeding, the SNP molecular marker is a nucleotide sequence as shown in SEQ ID NO.1 formed by single nucleotide mutation at the 686th site of the nucleotide sequence of the QNE1 gene in a soybean genome; the polymorphism of the single nucleotide mutation is C / G polymorphism. The method is used for assisting soybean breeding. The SNP marker provided by the invention can be used for molecular marker-assisted selection of soybean branch number related traits, and a key tool is provided for cultivating soybean varieties adapting to different planting requirements (such as a few-branch close planting type or a multi-branch high-yield type).

The invention belongs to the technical field of biology, and discloses a megalobrama amblycephala hypoxia-resistant snp molecular marker and detection and application thereof, the number of SNP sites of the SNP molecular marker related to megalobrama amblycephala hypoxia-resistant characters is three, the SNP sites are located at the 14321966 nucleotide site, the 14323457 nucleotide site and the 14323789 nucleotide site of a megalobrama amblycephala reference genome CM032046.1 respectively, the polymorphisms are G / A, T / C and C / A respectively, and the SNP sites are named as SNP1, SNP2 and SNP3 respectively. Based on the SNP molecular marker, the hypoxia-resistant character of megalobrama amblycephala can be identified by detecting the genotype of the SNP site, and the hypoxia-resistant megalobrama amblycephala can be screened, so that the breeding of the hypoxia-resistant variety of megalobrama amblycephala is accelerated, and the stable development of the megalobrama amblycephala culture industry is promoted.

The invention discloses a wheat grain number related C783T SNP (single nucleotide polymorphism) and application thereof, the SNP site corresponds to the 783th basic group from the 5'tail end of the sequence shown as SEQ ID NO: 1, namely the C783T SNP site; the sites are C / T polymorphisms. The invention also develops a reagent or a kit for identifying or assisting in identifying the character of the number of wheat ears based on the SNP site, and provides a method for identifying or assisting in identifying the genotype of wheat. Through experimental verification of a large number of wheat materials, it is found that the grain number of wheat with the homozygous genotype C / C is larger than or candidate larger than the grain number of wheat with the homozygous genotype T / T. The SNP has high effectiveness and potential application value, wheat with high grain number per ear can be found by detecting the SNP, and the SNP has important value in research or application of cultivation of high-yield wheat varieties.