26results about How to "High polymorphism" patented technology

The invention discloses a soybean SNP molecular marker combination, a chip and application thereof, and relates to the field of soybean molecular genetic breeding. The SNP molecular marker combination developed by the invention is uniformly distributed on 20 chromosomes of a soybean whole genome, and the accuracy of related research application such as gene positioning can be improved. The SNP molecular marker combination provided by the invention is rich in information amount and has important value in variety identification, correlation analysis and molecular marker-assisted breeding. On the basis, detection products such as SNP typing chips, kits and the like which are high in capture rate, high in density and high in polymorphism and are uniformly distributed in a genome can be developed.

The invention relates to the technical field of plant germplasm resources. The invention provides an SSR-PCR (Simple Sequence Repeat-Polymerase Chain Reaction) primer group for a robinia pseudoacacia genome SSR molecular marker, a robinia pseudoacacia core germplasm fingerprint spectrum obtained by utilizing the primer group and a method for constructing a robinia pseudoacacia core germplasm bank. The SSR-PCR primer group disclosed by the invention is relatively high in polymorphism, good in stability, relatively good in expression in the operation of the SSR molecular marker of the robinia pseudoacacia germplasm genome, and good in screening effect. By adopting the construction method disclosed by the invention, excellent germplasm with special characters can be screened out to construct the fingerprint spectrum of the robinia pseudoacacia core germplasm, a basis is better provided for scientific management, efficient utilization and preservation of robinia pseudoacacia germplasm resources, and meanwhile, the breeding efficiency can be effectively improved.

The invention discloses a primer combination developed based on idesia polycarpa whole genome sequencing and application of the primer combination, and belongs to the technical field of molecular biology. The idesia polycarpa primer combination comprises 18 pairs of SSR primer pairs and / or 30 IRAP primers. Through multiple rounds of screening and verification, the idesia primer combination has the characteristics of codominance, high repeatability, high polymorphism, good stability and the like, can be applied to idesia genetic diversity analysis and genetic relationship analysis, and can also be used for idesia and related genus germplasm genetic identification, breeding and the like. The SSR primer pair and the IRAP primer in the primer combination can be used independently or combined for comprehensive analysis, a more reliable molecular marker is provided for idesia genetic diversity analysis and variety genetic relationship analysis, and the primer combination has important theoretical and practical values.

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to soybean mosaic virus resistance, an amplification primer and application of the SNP molecular marker in soybean breeding. The SNP molecular marker is located at the 910489bp site of a No.7 chromosome of a soybean genome, and the polymorphism of the SNP molecular marker is C / A. The invention provides application of the SNP molecular marker related to the soybean mosaic virus resistance in identification or auxiliary identification of the soybean mosaic virus resistance. By providing the accurate, simple, convenient and low-cost SNP molecular marker and the detection method thereof, early-stage, rapid and efficient molecular-assisted selection of soybean mosaic virus resistance characters is realized, so that the breeding process of a new variety of disease-resistant soybeans can be remarkably accelerated, and the SNP molecular marker has important breeding application value.

The invention provides an MNP marker site for identifying various swine pathogens, a primer group, a kit and application, and belongs to the technical field of biology. And the MNP marker site comprises at least one of MNP-1 to MNP-18. The primer group comprises at least one of a first primer pair to an eighteenth primer pair, each primer pair comprises a forward primer and a reverse primer, and the forward primer of the first primer pair, the reverse primer of the first primer pair to the forward primer of the eighteenth primer pair and the reverse primer of the eighteenth primer pair are sequentially shown as SEQ ID NO: 1 to SEQ ID NO: 36 in a sequence table. The invention provides MNP marker sites for identifying various pig pathogens, a primer group, a kit and application, the primer group aims at 18 MNP marker sites, 1-3 marker sites are detected for each pathogen, 18 (types) pig pathogens can be identified, and the primer group has a comprehensive, efficient, sensitive and type distinguishing detection effect and is suitable for identifying various pig pathogens.

The present invention relates to the field of molecular biology, and in particular to polymorphic EST-SSR molecular marker primers for longitudinal pine and their applications. The nucleotide sequence of the primer set is shown in SEQ ID NO.1-SEQ ID NO.40. The primer set provided by the present invention can specifically amplify the EST-SSR molecular markers, and has a high degree of polymorphism, and can be applied to research fields such as longitudinal pine genetic diversity and population genetic structure. Moreover, the EST-SS molecular markers amplified by the EST-SSR primer set of the present invention have the advantages of abundant quantity, high polymorphism and good stability. At the same time, the amplification experiment of the EST-SSR primer set in the present invention is simple to operate, and the bands are clear and easy to distinguish, which is suitable for genetic diversity and genetic structure analysis of longitudinal pine.

This invention belongs to the field of Taihang flower research. It discloses 15 pairs of specific SSR primers for Taihang flower, a national second-class protected plant and endangered species, and their applications in the analysis of genetic diversity and genetic structure characteristics of the endangered species Taihang flower population. This can reveal the phylogenetic and evolutionary relationship of Taihang flower populations and has important theoretical significance for the research and protection of the endangered species Taihang flower.

The invention belongs to the technical field of molecular marker technology development and application, and discloses an SSR molecular marker developed based on a mactra veneriformis reference transcriptome sequence, and the SSR molecular marker comprises 16 nucleotide sequences corresponding to microsatellite markers as shown in SEQ ID NO.1-SEQ ID NO.16 in sequence. At present, no report about development of mactra veneriformis microsatellites from genomes and transcriptomes exists, 16 stable microsatellite molecular markers with clear strips and high polymorphism are developed from mactra veneriformis transcriptome data on the basis of a high-throughput transcriptome sequencing technology for the first time, and the microsatellite molecular markers can be used for identifying the mactra veneriformis microsatellites. The method has good practical production and application values in the fields of mactra veneriformis population genetics analysis, genetic relationship identification, high-density genetic map construction and molecular marker-assisted breeding.

This invention belongs to the field of ecological restoration and aquatic plant germplasm identification technology. It provides specific primers and identification methods for *Myriophyllum sieboldii* and *Myriophyllum sieboldii*, based on microsatellite marker (SSR) technology. Ten pairs of highly polymorphic microsatellite primers were screened and used to form five sets of dual PCR primer combinations for amplifying DNA fragments of the target species. Using confirmed *Myriophyllum sieboldii* and *Myriophyllum sieboldii* as basic reference samples, a genetic clustering model was constructed. DNA extraction, PCR amplification, electrophoresis analysis, and UPGMA clustering analysis were performed on the individuals to be identified, thereby achieving accurate species identification of suspected individuals. This invention enables rapid, accurate, and low-cost germplasm identification during the plant's vegetative growth stage and is applicable to wetland ecological restoration, germplasm resource protection, and aquatic vegetation surveys.

The invention provides a closely linked SSR marker and application thereof in barley helminthosporium leaf spot resistant QTL positioning, and relates to the technical field of molecular marker assisted breeding. The two QTL sites closely linked with the helminthosporium leaf spot resistance character are successfully positioned through the SSR marker technology, the accuracy of the SSR marker is further verified, the positioning work of the barley helminthosporium leaf spot resistance QTL is successfully completed within a short time by utilizing the SSR marker technology, timely and effective support is provided for disease-resistant breeding, and the method has the advantages of being high in practicability and high in practicability. According to the calculated QTL effect, the position of the QTL on the genetic map is determined, fine positioning of the QTL can be achieved, cross breeding is conducted through the screened helminthosporium leaf spot resistant genotype, a new helminthosporium leaf spot resistant barley variety can be more quickly cultivated in combination with a traditional breeding method, and the strategy of the modern genetic technology and the traditional breeding method is combined.

The application discloses a kind of SSR molecular marker primer for identifying Cymbidium varieties, and its application, the Cymbidium includes Cymbidium kanran, Cymbidium ensifolium, Cymbidium goeringii, Cymbidium sinense, Cymbidium devonianum, Cymbidium lancilobatum, Cymbidium kanran, hybrid orchid and hybrid Cymbidium. The SSR molecular marker includes SSR1-SSR23, and the primer sequence is shown as SEQ ID NO.1-SEQ ID NO.46 in the sequence table. The screening method of the SSR molecular marker primer is also included, which comprises the following steps of total DNA extraction of Cymbidium, SSR site selection and primer design based on Cymbidium reference genome, PCR amplification, polyacrylamide gel electrophoresis primary screening and ABI3730XL gene analyzer secondary screening. The application also provides a composition comprising the primer and its application in Cymbidium genetic relationship analysis, DUS specificity test and molecular marker assisted breeding. The SSR molecular marker primer provided by the application has the characteristics of high polymorphism, large quantity, low cost, easy reading, and is not limited by environment, and can quickly and accurately construct the DNA fingerprint of Cymbidium varieties.

The invention belongs to the field of cassava molecular markers, and particularly relates to a cassava KASP marker and application of the cassava KASP marker in cassava variety distinguishing. The 27 KASP markers provided by the invention have good distinguishing ability in cassava genetic analysis, and can effectively reveal genetic differences among different materials. The DNA fingerprint spectrum of 184 parts of cassava materials is constructed based on 27 core KASP markers, and the purpose of rapid variety identification can be achieved. The KASP marker disclosed by the invention can be used for effectively identifying false selfing offspring. In conclusion, the KASP marker provided by the invention provides a new method and thought for genetic improvement and variety identification of cassava, provides a powerful tool for subsequent cassava breeding and genetic improvement research, and is beneficial to breeding of new varieties with excellent characters.

The invention belongs to the field of biotechnology, and discloses a SNP molecular marker closely linked to the silique length and grain weight gene BnARF18 of Brassica napus and its application. The SNP site of the SNP molecular marker is located in the silique length and grain weight gene BnARF18, and is used for the accurate identification of the mutant type of the silique length and grain weight gene BnARF18. This marker has high typing quality, single copy, high polymorphism, and a sample data detection rate of> 98%. It can be used for marker-assisted breeding for breeding improvement of silique length and grain weight of Brassica napus, and has wide application universality. Also disclosed is the application of KASP marker primers and kits for the above-mentioned SNP molecular markers to identify the silique length and grain weight gene BnARF18 of Brassica napus, and the method is simple, the degree of automation is high, the detection flux is high, the speed is fast, the detection result is accurate, and the repeatability and stability are good.

The present invention provides a highly polymorphic microsatellite molecular marker of Penaeus vannamei and an application thereof, belonging to the technical field of molecular markers. The present invention is based on 10 microsatellite sequences screened from the genome of Penaeus vannamei, which are numbered Lv‑449, Lv‑460, Lv‑499, Lv‑511, Lv‑442, Lv‑451, Lv‑496, Lv‑491, Lv‑439 and Lv‑482, respectively, and their sequences are shown in SEQ ID No.1 to SEQ ID No.10. The present invention designs microsatellite marker primers for the 10 microsatellite sequences respectively, and the microsatellite marker primers have strong specificity, stable amplification and high polymorphism, and can be used for the genetic diversity and genetic structure analysis of Penaeus vannamei populations and the identification of germplasm resources, providing a powerful tool for the genetic breeding of Penaeus vannamei.

The invention belongs to the technical field of molecular biology, and discloses a molecular marker combination for identifying wax gourd germplasm resources, the molecular marker combination comprises 48 SNP molecular markers, the physical positions of the 48 SNP molecular markers are determined by sequence alignment based on a wax gourd reference genome GCF009727055.1, and the chromosome positions and base variation of the 48 SNP molecular markers are shown in the specification table 1. The invention further discloses a primer group for detecting the molecular marker combination, the primer group comprises 48 pairs of primers, and the sequence of each pair of primers is shown in the specification table 2. The invention also discloses a kit which comprises the primer group. The invention also discloses applications of the molecular marker combination, the primer group and the kit. According to the molecular marker combination for identifying the wax gourd germplasm resources, 48 SNP markers are screened, and the 48 molecular markers are high in typing quality, single in copy and high in polymorphism.

This invention belongs to the field of biotechnology and provides an application of SSR primers in the identification of cauliflower germplasm resources. The application includes using the SSR primers for cauliflower variety identification, phylogenetic evaluation, cultivation of new cauliflower germplasm, and purity identification of cauliflower hybrids. The 29 pairs of SSR primers screened in this invention exhibit clear amplification banding, good stability, and high polymorphism, making them highly suitable for cauliflower variety identification, phylogenetic evaluation, cultivation of new cauliflower germplasm, and purity identification of cauliflower hybrids.

This disclosure provides an MNP marker site, primer set, kit, and application for identifying multiple pathogens in wastewater. The MNP marker site includes at least one of MNP-1 to MNP-19. The primer set includes at least one primer pair from the first to the 19th primer pair. The forward primer of the first primer pair, the reverse primer of the first primer pair, the forward primer of the 19th primer pair, and the reverse primer of the 19th primer pair are shown sequentially as SEQ ID NO: 1 to SEQ ID NO: 38 in the sequence listing. This disclosure provides an MNP marker site, primer set, kit, and application for identifying multiple pathogens in wastewater. The primer set targets 19 MNP marker sites, and each pathogen can be detected by 1 to 3 marker sites. It can identify multiple pathogens in wastewater, providing comprehensive, efficient, and accurate detection, and is suitable for identifying multiple pathogens in wastewater.

The present invention belongs to the technical field of molecular biology, and specifically relates to an SSR molecular marker primer for identifying Cattleya varieties and its application. The Cattleya includes closely related genera such as Phalaenopsis, Dendrobium, and Cymbidium; the SSR molecular marker for identifying Cattleya varieties includes SSR1 to SSR50, and their nucleic acid sequences are shown in the sequence table SEQ ID NO.1 to SEQID NO.100, respectively. Compared with other molecular markers, the SSR molecular marker primer for identifying Cattleya varieties of the present invention and its composition have the characteristics of high polymorphism, large quantity and low cost. The selected Cattleya materials are not limited by season, environment and test time, and DNA can be extracted from any tissue in any growth period of the variety or samples after refrigerated storage for a period of time without affecting the identification results.

The invention belongs to the field of biotechnology, and discloses an application of a group of SNP molecular markers as detection targets in identifying Chinese pumpkin germplasm resources. The invention utilizes genome resequencing information of pumpkin germplasm resources, selects representative sites with good polymorphism from millions of SNP sites based on specific parameters and conditions, screens out 25 core SNP sites for developing SNP molecular markers, and then obtains 25 core SNP molecular markers with high polymorphism and uniform distribution on each chromosome and converts them into KASP primers; the invention can lay an important technical support for the identification of pumpkin germplasm resources, the construction of pumpkin DNA fingerprint map, pumpkin kinship analysis, pumpkin variety right protection, etc.

The invention discloses azalea SSR molecular marker, a primer composition and an application thereof, and belongs to the technical field of azalea molecular marker. The invention provides a total of 30 SSR molecular markers, of which 6 have dibase repeating units, 24 have tribase repeating units, 30 pairs of primers related to molecular markers, and the provided SSR and primer composition have clear amplified bands, high polymorphism, and good repeatability in various varieties of azalea, and can be used for germplasm identification, genetic diversity and kinship analysis among various subgenera of azalea, with stable and reliable results, laying a foundation for the management of germplasm resources of azalea, molecular marker-assisted breeding, genetic protection, and genetic diversity analysis among and within azalea populations; and also has the advantages of being simple to use and highly efficient.

This invention discloses a 6K liquid-phase breeding chip for broccoli, its dedicated single nucleotide polymorphism (SNP) variant sites, and its applications. The 6K liquid-phase breeding chip provided by this invention includes a probe combination for detecting 5971 SNP variant sites in the broccoli genome. The location information of the probe corresponding to each SNP variant site in the broccoli reference genome is shown in Table 3 of the specification. The SNP variant sites involved in the liquid-phase breeding chip provided by this invention have advantages such as high polymorphism, good repeatability, stable and reliable labeling, and ease of statistical analysis. This invention can also establish a DNA fingerprint database based on the liquid-phase breeding chip for identifying the authenticity of broccoli varieties. It can be used for identifying the authenticity of broccoli varieties, early breeding and identification of broccoli varieties, identification of broccoli seed resources, and provides technical support for enriching broccoli germplasm resources, breeding new varieties, and protecting new varieties, with broad application prospects.

The invention relates to the technical field of plant breeding, in particular to SSR molecular markers of malus plants and application of the SSR molecular markers. The molecular marker comprises 17 SSR (Simple Sequence Repeat) marker sites on chromosomes 1-17 of an apple. 17 long repeat motif (4-6 nucleotide repeat) SSR markers with high polymorphism, high reliability, good repeatability and good universality are screened from seven published whole genome sequences of Malus plants, and the molecular markers can be used for genetic research and application of DNA fingerprint spectrum construction, molecular identification, diversity evaluation, resource protection and the like of the Malus plants. The method has important significance in the field of apple plant breeding.

The invention provides an MNP marker site for identifying multiple human respiratory pathogens, a primer group, a kit and application, and belongs to the technical field of biology. The MNP marker site comprises at least one of MNP-1 to MNP-17, the primer group comprises at least one of a first primer pair to a seventeenth primer pair, and each primer pair comprises a forward primer and a reverse primer, a forward primer of the first primer pair, a reverse primer of the first primer pair, a forward primer of the 17th primer pair and a reverse primer of the 17th primer pair are sequentially shown as SEQ ID NO: 1 to SEQ ID NO: 34 in a sequence table. The invention provides MNP marker sites for identifying multiple human respiratory pathogens, a primer group, a kit and application, the primer group aims at 17 MNP marker sites, 1-3 marker sites are detected for each pathogen, 13 human respiratory pathogens can be identified, and the primer group has a comprehensive, efficient and accurate detection effect and is suitable for identifying multiple human respiratory pathogens.

The invention discloses an SNP (Single Nucleotide Polymorphism) genetic marker system for genetic relationship identification of a highly degraded sample, a detection primer and application of the SNP genetic marker system and the detection primer, and belongs to the technical field of forensic medicine identification. According to the invention, through bioinformatics screening and experimental verification, a triallelic SNP genetic marker system of which the lengths of 25 amplicons all do not exceed 110 bp is constructed. The problem that it is difficult to give consideration to genetic marker typing and high system efficiency of highly degraded samples is solved. The random matching probability of the system in Han population in Hunan reaches 1.59 * 10 <-18 >, the cumulative paternity exclusion rate is 0.99979, complete typing of highly degraded DNA can be realized, triad paternity test can be completed, and a new technical scheme which is efficient and compatible with a capillary electrophoresis platform is provided for forensic practice.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination for identifying germplasm resources of red beans and application of the SNP molecular marker combination, and belongs to the technical field of molecular biology. According to the invention, 18 SNP loci with high polymorphism and remarkable resolving power are excavated and screened out, a primer combination for specific detection is developed, a standardized molecular detection system is formed in cooperation with optimized unified PCR detection conditions, and the primer combination shows excellent identification capability in red bean germplasm resource identification and has a good application prospect in the field of red bean germplasm resource identification. According to the method, red bean resources with different genetic backgrounds can be clearly and accurately distinguished, it is proved that the method has high effectiveness and reliability in practical application, and rich marker resources and reliable technical support are provided for research of red bean genetic map construction, important character gene positioning, fingerprint development, molecular marker-assisted selection and the like.

The invention relates to the technical field of brassica napus gene detection, in particular to a brassica napus 1.6 k whole genome molecular marker combination and application thereof. According to the invention, SNP loci of resequencing data of 624 important rape germplasm resources are analyzed, 1597 high-quality whole genome molecular markers and gene function linkage markers with high polymorphism, high detection rate and uniform distribution on chromosomes are screened, specific primer design is carried out on the screened SNP / INDEL loci, and the SNP / INDEL molecular markers can be used for screening the whole genome molecular markers and gene function linkage markers. Specific amplification is simultaneously carried out on thousands of target sites in the same PCR reaction by utilizing a specific primer through multiple PCR, and then sequencing library construction and next-generation sequencing are carried out on PCR amplicons, so that genotypes of different target sites are obtained. The kit is used for detecting the SNP locus of the brassica napus, is high in sample detection flexibility, low in cost and short in period, and can be popularized to seed production and application.