11results about How to "High polymorphism" patented technology

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to soybean mosaic virus resistance, an amplification primer and application of the SNP molecular marker in soybean breeding. The SNP molecular marker is located at the 910489bp site of a No.7 chromosome of a soybean genome, and the polymorphism of the SNP molecular marker is C / A. The invention provides application of the SNP molecular marker related to the soybean mosaic virus resistance in identification or auxiliary identification of the soybean mosaic virus resistance. By providing the accurate, simple, convenient and low-cost SNP molecular marker and the detection method thereof, early-stage, rapid and efficient molecular-assisted selection of soybean mosaic virus resistance characters is realized, so that the breeding process of a new variety of disease-resistant soybeans can be remarkably accelerated, and the SNP molecular marker has important breeding application value.

This invention belongs to the field of Taihang flower research. It discloses 15 pairs of specific SSR primers for Taihang flower, a national second-class protected plant and endangered species, and their applications in the analysis of genetic diversity and genetic structure characteristics of the endangered species Taihang flower population. This can reveal the phylogenetic and evolutionary relationship of Taihang flower populations and has important theoretical significance for the research and protection of the endangered species Taihang flower.

This invention belongs to the field of ecological restoration and aquatic plant germplasm identification technology. It provides specific primers and identification methods for *Myriophyllum sieboldii* and *Myriophyllum sieboldii*, based on microsatellite marker (SSR) technology. Ten pairs of highly polymorphic microsatellite primers were screened and used to form five sets of dual PCR primer combinations for amplifying DNA fragments of the target species. Using confirmed *Myriophyllum sieboldii* and *Myriophyllum sieboldii* as basic reference samples, a genetic clustering model was constructed. DNA extraction, PCR amplification, electrophoresis analysis, and UPGMA clustering analysis were performed on the individuals to be identified, thereby achieving accurate species identification of suspected individuals. This invention enables rapid, accurate, and low-cost germplasm identification during the plant's vegetative growth stage and is applicable to wetland ecological restoration, germplasm resource protection, and aquatic vegetation surveys.

The application discloses a kind of SSR molecular marker primer for identifying Cymbidium varieties, and its application, the Cymbidium includes Cymbidium kanran, Cymbidium ensifolium, Cymbidium goeringii, Cymbidium sinense, Cymbidium devonianum, Cymbidium lancilobatum, Cymbidium kanran, hybrid orchid and hybrid Cymbidium. The SSR molecular marker includes SSR1-SSR23, and the primer sequence is shown as SEQ ID NO.1-SEQ ID NO.46 in the sequence table. The screening method of the SSR molecular marker primer is also included, which comprises the following steps of total DNA extraction of Cymbidium, SSR site selection and primer design based on Cymbidium reference genome, PCR amplification, polyacrylamide gel electrophoresis primary screening and ABI3730XL gene analyzer secondary screening. The application also provides a composition comprising the primer and its application in Cymbidium genetic relationship analysis, DUS specificity test and molecular marker assisted breeding. The SSR molecular marker primer provided by the application has the characteristics of high polymorphism, large quantity, low cost, easy reading, and is not limited by environment, and can quickly and accurately construct the DNA fingerprint of Cymbidium varieties.

The invention belongs to the field of cassava molecular markers, and particularly relates to a cassava KASP marker and application of the cassava KASP marker in cassava variety distinguishing. The 27 KASP markers provided by the invention have good distinguishing ability in cassava genetic analysis, and can effectively reveal genetic differences among different materials. The DNA fingerprint spectrum of 184 parts of cassava materials is constructed based on 27 core KASP markers, and the purpose of rapid variety identification can be achieved. The KASP marker disclosed by the invention can be used for effectively identifying false selfing offspring. In conclusion, the KASP marker provided by the invention provides a new method and thought for genetic improvement and variety identification of cassava, provides a powerful tool for subsequent cassava breeding and genetic improvement research, and is beneficial to breeding of new varieties with excellent characters.

The invention belongs to the technical field of molecular biology, and discloses a molecular marker combination for identifying wax gourd germplasm resources, the molecular marker combination comprises 48 SNP molecular markers, the physical positions of the 48 SNP molecular markers are determined by sequence alignment based on a wax gourd reference genome GCF009727055.1, and the chromosome positions and base variation of the 48 SNP molecular markers are shown in the specification table 1. The invention further discloses a primer group for detecting the molecular marker combination, the primer group comprises 48 pairs of primers, and the sequence of each pair of primers is shown in the specification table 2. The invention also discloses a kit which comprises the primer group. The invention also discloses applications of the molecular marker combination, the primer group and the kit. According to the molecular marker combination for identifying the wax gourd germplasm resources, 48 SNP markers are screened, and the 48 molecular markers are high in typing quality, single in copy and high in polymorphism.

This invention belongs to the field of biotechnology and provides an application of SSR primers in the identification of cauliflower germplasm resources. The application includes using the SSR primers for cauliflower variety identification, phylogenetic evaluation, cultivation of new cauliflower germplasm, and purity identification of cauliflower hybrids. The 29 pairs of SSR primers screened in this invention exhibit clear amplification banding, good stability, and high polymorphism, making them highly suitable for cauliflower variety identification, phylogenetic evaluation, cultivation of new cauliflower germplasm, and purity identification of cauliflower hybrids.

This disclosure provides an MNP marker site, primer set, kit, and application for identifying multiple pathogens in wastewater. The MNP marker site includes at least one of MNP-1 to MNP-19. The primer set includes at least one primer pair from the first to the 19th primer pair. The forward primer of the first primer pair, the reverse primer of the first primer pair, the forward primer of the 19th primer pair, and the reverse primer of the 19th primer pair are shown sequentially as SEQ ID NO: 1 to SEQ ID NO: 38 in the sequence listing. This disclosure provides an MNP marker site, primer set, kit, and application for identifying multiple pathogens in wastewater. The primer set targets 19 MNP marker sites, and each pathogen can be detected by 1 to 3 marker sites. It can identify multiple pathogens in wastewater, providing comprehensive, efficient, and accurate detection, and is suitable for identifying multiple pathogens in wastewater.

This invention discloses a 6K liquid-phase breeding chip for broccoli, its dedicated single nucleotide polymorphism (SNP) variant sites, and its applications. The 6K liquid-phase breeding chip provided by this invention includes a probe combination for detecting 5971 SNP variant sites in the broccoli genome. The location information of the probe corresponding to each SNP variant site in the broccoli reference genome is shown in Table 3 of the specification. The SNP variant sites involved in the liquid-phase breeding chip provided by this invention have advantages such as high polymorphism, good repeatability, stable and reliable labeling, and ease of statistical analysis. This invention can also establish a DNA fingerprint database based on the liquid-phase breeding chip for identifying the authenticity of broccoli varieties. It can be used for identifying the authenticity of broccoli varieties, early breeding and identification of broccoli varieties, identification of broccoli seed resources, and provides technical support for enriching broccoli germplasm resources, breeding new varieties, and protecting new varieties, with broad application prospects.

The invention discloses an SNP (Single Nucleotide Polymorphism) genetic marker system for genetic relationship identification of a highly degraded sample, a detection primer and application of the SNP genetic marker system and the detection primer, and belongs to the technical field of forensic medicine identification. According to the invention, through bioinformatics screening and experimental verification, a triallelic SNP genetic marker system of which the lengths of 25 amplicons all do not exceed 110 bp is constructed. The problem that it is difficult to give consideration to genetic marker typing and high system efficiency of highly degraded samples is solved. The random matching probability of the system in Han population in Hunan reaches 1.59 * 10 <-18 >, the cumulative paternity exclusion rate is 0.99979, complete typing of highly degraded DNA can be realized, triad paternity test can be completed, and a new technical scheme which is efficient and compatible with a capillary electrophoresis platform is provided for forensic practice.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination for identifying germplasm resources of red beans and application of the SNP molecular marker combination, and belongs to the technical field of molecular biology. According to the invention, 18 SNP loci with high polymorphism and remarkable resolving power are excavated and screened out, a primer combination for specific detection is developed, a standardized molecular detection system is formed in cooperation with optimized unified PCR detection conditions, and the primer combination shows excellent identification capability in red bean germplasm resource identification and has a good application prospect in the field of red bean germplasm resource identification. According to the method, red bean resources with different genetic backgrounds can be clearly and accurately distinguished, it is proved that the method has high effectiveness and reliability in practical application, and rich marker resources and reliable technical support are provided for research of red bean genetic map construction, important character gene positioning, fingerprint development, molecular marker-assisted selection and the like.