120 results about "Rice plants" patented technology

The invention discloses application of OsHTT6 protein in regulation and control of high temperature stress tolerance and yield of rice. Through construction of an OsHTT6 protein function deletion mutant rice line and an OsHTT6 protein overexpression transgenic rice line, it is found that by inhibiting expression of the OsHTT6 protein, the tolerance of rice to high temperature stress can be improved, the survival rate of rice seedlings subjected to high temperature stress is increased, in addition, the yield of a single plant of rice can be increased, and the yield of the rice seedlings is increased. And overexpression of the OsHTT6 protein can reduce the tolerance of the rice to high temperature stress and reduce the single-plant yield of the rice. The high-temperature stress tolerance and yield of the rice can be regulated and controlled by regulating and controlling the expression of the OsHTT6 protein, and a reagent capable of inhibiting the expression of the OsHTT6 protein can be used for improving the high-temperature stress tolerance and yield of the rice or constructing heat-resistant and high-yield rice plants. The method is beneficial to cultivation of heat-resistant and high-yield rice varieties.

The invention belongs to the field of plant genetic engineering, and particularly relates to application of an allele of a rice leaf included angle gene OsPUB77. The invention provides application of rice leaf included angle alleles OsPUB77R and OsPUB77I, and the application is at least one of the following applications: regulating and controlling a rice leaf included angle and improving a rice plant type; the nucleotide sequence of the rice leaf included angle allele OsPUB77R is as shown in SEQ ID NO: 1; the nucleotide sequence of the rice leaf included angle allele OsPUB77I is as shown in SEQ ID NO: 3. The rice containing the allele OsPUB77R is small in leaf included angle and compact in plant type; the rice containing the allele OsPUB77I is large in leaf included angle and loose in plant type.

The invention relates to the technical field of biology, in particular to application of an OgRKa gene and an upstream promoter thereof to improvement of rice bacterial blight and rice blast resistance, nucleotide sequences of the OgRKa gene and the upstream promoter are as shown in SEQ ID No.1, a CDS sequence is as shown in SEQ ID No.4, and an amino acid sequence of an encoding protein OgRKa is as shown in SEQ ID No.5. The homologous gene OgRKa of the rice MRKa gene (a rice plant over-expressing the MRKa gene has partial resistance to the rice bacterial blight, and a rice plant expressing the MRKa gene by using an MRKa self promoter has no resistance to the rice bacterial blight) is homologous cloned from oryza meyeriana through PCR (Polymerase Chain Reaction) amplification. Compared with the prior art, the resistance of rice plants with the OgRKa gene overexpressed by the 35S promoter or the OgRKa gene expressed by the promoter per se to a plurality of xanthomonas oryzae and magnaporthe oryzae is remarkably enhanced, the approximate immune level is achieved, the broad-spectrum high-resistance xanthomonas oryzae and magnaporthe oryzae are shown, and good application prospects are achieved in the aspect of breeding of xanthomonas oryzae and magnaporthe oryzae resistant varieties.

The invention relates to the field of ustilaginoidea virens prevention and treatment, and discloses a novel green ustilaginoidea virens prevention and treatment method which comprises the following steps: S1, irradiating seeds through a sterilization waveband ultraviolet lamp; s2, spraying the biological agent to rice plants in the booting stage of the rice; s3, selecting matrine plant extract to spray rice plants at a key stage when the rice is close to a crevasse and is about to enter a susceptible disease; s4, applying the electrolyzed water generator to prepare weakly acidic electrolyzed water in the heading stage of the rice, and spraying the weakly acidic electrolyzed water to the rice plants. Green prevention and control means such as ultraviolet sterilization, biological agents, plant extracts and electrolyzed water are adopted, the base number of pathogenic bacteria is reduced by treating seeds through ultraviolet rays, chemical residues are not introduced, bacillus subtilis serves as the biological agents, bacteria are used for bacteriostasis, matrine plant extracts are natural, and the environment-friendly effect is achieved. The weakly acidic and weakly alkaline electrolyzed water prevents and controls diseases by virtue of unique physicochemical properties, and is pollution-free.

Herbicide-resistant rice plants, isolated polynucleotides that encode herbicide resistant and wild-type acetohydroxy-acid synthase large subunit 1 (AHASL1) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to imidazolinone herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.

The utility model relates to the technical field of rice planting, in particular to a pesticide spraying device for rice planting. The pesticide spraying device comprises a main body frame, a fixing assembly is arranged on the inner wall of the main body frame, a protection assembly is arranged on the surface of the fixing assembly, the fixing assembly is used for fixing the protection assembly, the protection assembly is used for preventing pesticide sprayed out of the main body frame from being interfered by the wind direction, and the protection assembly comprises a protection frame arranged on the surface of the fixing assembly. By arranging the windproof frame, pesticide spraying can be effectively prevented from deviating from the preset spraying direction and range due to natural wind, it is ensured that all rice plants can receive enough pesticide, the pest control effect is improved, waste and environmental pollution caused by excessive spraying of pesticide are reduced, sprayed pesticide droplets are dispersed more evenly, and the pesticide spraying effect is improved. And the windproof frame in the protection assembly is made of organic glass, so that the windproof frame has good transparency and is relatively light.

The invention discloses bacillus velezensis as well as a biological preparation and application thereof, and belongs to the technical field of microorganisms. The bacillus velezensis is rice endophytic bacillus velezensis, is classified and named as bacillus velezensis HA-B13, is preserved in the China Center for Type Culture Collection (CCTCC), has the preservation number of CCTCC NO: M 20251098, shows extremely excellent biological activity to nigatosporium oryzae, has the antibacterial rate of 94%, and can be used for preparing the bacillus velezensis. Meanwhile, the bacillus subtilis also has relatively good biological activity on a plurality of rice pathogenic bacteria such as rhizoctonia solani, pyricularia grisea, gylastoma gonoryzae and the like. And the strain is derived from rice plants and can be well colonized in the rice plants, so that the strain has a long-acting prevention and treatment effect on diseases such as spike rot and the like, can effectively reduce the investment of rice disease prevention and treatment products, is safer and has great prevention and treatment application potential.

The invention discloses a saline-alkaline tolerance gene introduced rice high-yield breeding method. The method comprises the following steps: S1, gene screening and cloning: screening related genes with saline-alkaline tolerance; s2, vector construction: inserting the screened saline-alkaline tolerant gene into a proper expression vector; s3, transforming rice cells: introducing the vector carrying the saline-alkaline tolerant gene into the rice cells; s4, screening positive plants: confirming that the rice cells with the saline-alkaline tolerant genes are successfully transferred, and selecting the positive plants from the rice cells for next-step culture; s5, tissue culture and development: placing the positive plants on a sterile culture medium for tissue culture, and enabling the positive plants to develop into complete plants; s6, planting and harvesting: transplanting and planting the obtained transgenic rice plants, and harvesting the transgenic rice plants to obtain rice seeds. The saline-alkali soil-resistant gene disclosed by the invention is stronger in saline-alkali resistance, the yield is not influenced after the gene is planted in saline-alkali soil, and high-yield rice can be obtained.

The invention discloses application of a rice zinc finger protein OsRFI2 and a coding gene thereof in disease-resistant breeding, and belongs to the field of gene engineering. The rice zinc finger protein OsRFI2 is identified, the amino acid sequence of the rice zinc finger protein OsRFI2 is as shown in SEQ ID NO.2, and the nucleotide sequence of the coding gene OsRFI2 is as shown in SEQ ID NO.1. According to the invention, Zhonghua-11 rice is taken as a background, a knockout plant and an overexpression plant of the OsRFI2 gene are constructed, inoculation experiment results show that the scab length of the overexpression plant of the OsRFI2 gene is significantly smaller than that of the background plant, the overexpression of the OsRFI2 gene significantly improves the resistance of the rice plant to rice blast, and the rice zinc finger protein OsRFI2 positively regulates the disease resistance of the plant. The invention provides a new breeding strategy and a new breeding material for rice disease-resistant breeding, and has great economic benefits and wide application prospects for rice production.

The invention discloses an ecological fertilization planting method and system for improving the selenium content in rice, which relates to the field of ecological fertilization planting, including: establishing a microalgae culture pool, using a selenium-rich water supply system to cultivate a specific microalgae species that can enrich selenium; analyzing the electrical signal characteristics of rice plants corresponding to different selenium contents at various growth stages, and establishing a plant selenium absorption electrical signal reaction pattern library; based on real-time detection of rice plant electrical signals and matching results of the plant selenium absorption electrical signal reaction pattern library, using an intelligent irrigation system to optimize and adjust the application amount of microalgae liquid supplied to the rice field; returning the remaining selenium-rich microalgae liquid to the culture pool for recycling, and dynamically regulating the selenium supplementation amount of the microalgae culture pool.

The invention provides a method for regulating and controlling the heavy metal content of rice through MORC6s and application, and particularly belongs to the technical field of rice cultivation. The invention provides a gene for regulating and controlling the heavy metal content of the root part and the overground part of rice. The gene comprises any one or more of MORC6a, MORC6b and MORC6c. The MORC6a gene, the MORC6b gene and the MORC6c gene are subjected to induced expression after being treated by the heavy metal cadmium for different times. The invention finds that the MORC6a, MORC6b and MORC6c genes play an important role in regulating and controlling the accumulation process of cadmium at the root part and the overground part of rice. By utilizing the MORC6a, MORC6b and MORC6c genes, the harm of soil cadmium pollution to the growth and development of rice plants can be relieved, the food safety problem caused by excessive cadmium content is relieved, effective reference is further provided for low-cadmium rice cultivation, and accumulation of genetic resources of the low-cadmium rice is enriched.

This invention discloses a cultivated rice from Africa pi- cnv9 The application of genes in improving rice disease resistance belongs to the field of genetic engineering technology. This invention has screened a gene associated with rice blast resistance. pi-cnv9 The gene, whose nucleotide sequence is shown in SEQ ID NO.1, was used in this invention to construct complementary transgenes for *Rhizophora flavescens* and *Rhizophora esculenta*, respectively. pi-cnv9 Rice plants with complementary genes. Inoculation experiments were conducted on wild-type and transgenic plants, and the results demonstrated that complementary transgenic... pi-cnv9 Genetically modified plants are more resistant to disease than wild-type plants; indicating that... pi-cnv9 Genes that positively regulate the disease resistance of rice can be used as target genes for genetic improvement and molecular breeding of rice resistance to rice blast, which is of great significance for breeding rice varieties with improved disease resistance.

The application discloses a method for preparing rice with changed tiller number, and belongs to the technical field of genetic breeding. The method comprises the following steps: introducing a coding gene of an OsD14 mutant into target rice to obtain rice with changed tiller number, wherein the tiller number of the rice with changed tiller number is higher or lower than that of the target rice, and the OsD14 mutant is a protein obtained by mutating the 10th, 11th, 13th and 15th amino acids in SEQ ID NO: 1 into other amino acids and keeping other amino acids in SEQ ID NO: 1 unchanged. It is proved by experiments that whether ubiquitination degradation of the OsD14 occurs is related to a phosphorylation state of the OsD14, the number of rice tillers is adjusted by using the phosphorylation state of the OsD14, and the plant type is improved, so that the yield of the rice is improved, and the method has a wide application prospect.

The invention discloses application of an ELSA gene to improvement of rice disease resistance. Researches show that knockout of an ELSA gene promoter region or a gene body region can improve the resistance of rice to bacterial leaf blight, bacterial streak and rice blast, has no significant influence on the plant type, panicle type and the like of rice, and maintains the stability of the rice yield, that is, the rice yield is not influenced. It is indicated that the ELSA gene is a negative regulation gene of rice bacterial leaf blight, bacterial streak and rice blast resistance. Therefore, the rice with high disease resistance and stable yield can be cultivated by knocking out the rice ELSA gene promoter region or gene body region, and a simple and effective method is provided for cultivating the rice with high disease resistance and stable yield.

The present invention discloses the use of the OsST1 gene in regulating rice salt tolerance. This invention belongs to the field of plant genetic engineering technology. The invention provides the use of the OsST1 gene in regulating rice salt tolerance, demonstrating for the first time the role of the rice OsST1 gene in rice salt tolerance. Research has shown that overexpressing the OsST1 gene can improve the salt tolerance of rice plants and their survival rate under salt stress, demonstrating that the OsST1 gene has a role in regulating plant salt tolerance and addresses the adverse effects of soil salinization on rice growth in practical applications. Furthermore, the verification of the gene's biological function is of great significance for research into the molecular mechanism of rice salt tolerance.

The present disclosure relates to a mutant sequence of rice OsABCD1 gene and a method and application thereof in regulating cadmium accumulation in rice. Specifically, it relates to a mutant sequence of rice OsABCD1 gene, a protein encoded by the mutant sequence, a vector, a transformant, a method for reducing cadmium accumulation in rice plants and the application thereof. The present disclosure makes the function of the gene lost by gene mutation at the target site I of rice OsABCD1 gene, thereby reducing the accumulation of cadmium in rice; provides gene resources and technical support for breeding rice varieties with reduced cadmium absorption content, effectively improves the problem of excessive cadmium content in rice, and has important agricultural application value.

The invention provides an OsWRKY19 gene and an application of the OsWRKY19 gene in resistance to cochliobolus miyabeanus of rice. A CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 (CRISPR associated protein 9) technology is adopted to obtain an OsWRKY19 knockout mutant (Oswrky19) and an overexpression strain of And after the cochliobolus miyabeanus pathogenic bacteria are inoculated, compared with a wild type, the knockout mutant is more susceptible to diseases, and the over-expression strain resistance is obviously enhanced. The OsWRKY19 overexpression obviously improves the callose deposition level after rice plants are induced by chitin, and further enhances the basic defensive reaction of rice. RT-qPCR discovers that the expression of the defensive reaction related gene OsMBL1 in a knockout mutant is reduced and is increased in an overexpression plant, which indicates that the OsWRKY19 can promote the expression of the OsMBL1, thereby enhancing the resistance of rice. In conclusion, the OsWRKY19 is a gene for positively regulating and controlling the resistance of the cochliobolus miyabeanus of the rice, and can promote the basic defense response of the rice and the expression of a disease-resistant related gene, so that the resistance of the rice to the cochliobolus miyabeanus is improved. The content of the invention provides important genetic resources and theoretical guidance for the breeding of the cochliobolus miyabeanus resistant variety of rice.

The invention discloses a composite plant growth regulator and a preparation method and application thereof, belonging to the field of plant growth regulators. The composite plant growth regulator comprises the following main components: sea buckthorn oil, brown cyanidin, triacontanol, uniconazole, and salicylic acid. The plant growth regulator of the invention adds sea buckthorn oil and brown cyanidin, and these two components are compounded with triacontanol, uniconazole and salicylic acid to form a composite plant growth regulator. The synergistic effect of multiple substances increases the number of effective panicles, the number of panicles and the thousand-grain weight of rice plants on the one hand, thereby increasing the yield; on the other hand, the internode length of the base of the rice is shortened, the plant height is shortened, the stalk is promoted to be thick, and the lodging resistance of the rice is enhanced. The plant growth regulator of the invention can be used alone as a plant growth regulator for increasing rice yield, reducing plant height and improving lodging resistance. In addition, the plant growth regulator of the invention has a wide source, low cost, simple preparation method, convenient use, and is easy to promote.

The invention discloses a method for determining the vitality of rice tiller buds and a method for detecting the level of active oxygen in tiller buds. The determination method determines the vitality of rice tiller buds by detecting the level of active oxygen ROS in the tiller buds of rice seedlings; the level of active oxygen ROS in the tiller buds of the rice seedlings is negatively correlated with the vitality of the tiller buds. When the active oxygen ROS accumulates more, the vitality of the rice tiller buds is poor; when the active oxygen ROS accumulates less, the vitality of the rice tiller buds is strong. The method for detecting the level of active oxygen ROS in tiller buds is to cultivate rice seedlings, obtain tiller bud tissues, perform active oxygen ROS staining observation, and determine and analyze the content of active oxygen ROS components superoxide anion and hydrogen peroxide, so as to evaluate the level of active oxygen ROS in rice tiller buds from qualitative and quantitative aspects. The present invention adds a new method to the study of the growth and development (tiller bud vitality) of tiller buds of rice and other crops, and the present invention also provides new ideas and insights for explaining the morphological construction of rice plants.

The invention discloses application of a nano-material composite plant growth regulator to improvement of rice stress resistance and inhibition of magnaporthe oryzae. The plant growth regulator is prepared from magnesium ions and a nano material ZIF-8. The conditioning agent has the advantages that the conditioning agent can effectively inhibit the growth of magnaporthe oryzae hyphae and the formation of appressorium, and the morbidity of the magnaporthe oryzae can be remarkably reduced by mixing the conditioning agent with magnaporthe oryzae conidia to be sprayed and inoculated to rice leaves; meanwhile, the regulator can activate an anti-oxidation defense system of rice and enhance the stress resistance of plants. After the fertilizer is applied in a magnesium-deficient environment, key physiological indexes such as plant height, chlorophyll content, stomatal conductance, normalized difference vegetation index, maximum photochemical efficiency and the like of rice plants can be remarkably improved, so that the growth state of the rice plants is recovered to a normal level. Biosafety verifies that the regulator is non-toxic and environment-friendly to rice, and pollution caused by traditional chemical pesticides can be reduced.

The invention provides a DSE chlamydospore water aqua as well as a preparation method and application thereof, and belongs to the technical field of microbial preparations, the DSE chlamydospore water aqua comprises chlamydospore from at least one dark partitioned endophytic fungus, and further comprises 5g / mL-10g / mL of preservative and 5g / mL-10g / mL of protective agent, and the content of the chlamydospore is greater than 2.5 * 10 < 8 > CFU / mL. The product property of the water aqua is improved by reasonably optimizing the dosage of DSE chlamydospore and auxiliary materials, the content of chlamydospore in the obtained water aqua finished product can reach 2.54 * 10 < 8 > CFU / mL, and all indexes meet the national standard; the conidium water aqua can be stored for more than 12 months at normal temperature, and the shelf life is prolonged by more than 9 months compared with a conventional conidium water aqua. The DSE chlamydospore aqueous solution can effectively prevent and treat tomato bacterial wilt and southern rice black-streaked dwarf disease, improve the growth vigor of tomato plants and rice plants, and achieve a prevention and treatment effect which is not inferior to or even superior to that of positive control trichoderma harzianum. The DSE chlamydospore aqueous solution provided by the invention is simple to prepare, controllable in cost and excellent in performance, and is suitable for large-scale popularization and application as a novel biopesticide product.

This invention discloses a method and microbial preparation for controlling mercury accumulation in rice grains using Metarhizium anisopliae colonization, belonging to the field of agricultural environment and microbiology technology. The method involves inoculating rice plants with Metarhizium anisopliae spores, followed by planting the rice in mercury-contaminated paddy fields; water management is implemented throughout the rice's growth cycle until harvest. This invention establishes a complete microbial remediation system for mercury contamination in paddy fields, providing not only a method for the colonization and application of Metarhizium anisopliae in mercury-contaminated environments, but also ensuring safe rice production, offering a feasible approach to safeguarding food security and human health.

The present invention belongs to the field of molecular biology, and specifically discloses an application of a rice OsERF52 protein and its encoding gene in improving plant low temperature tolerance, and relates to the field of plant genetic engineering technology. The amino acid sequence of the rice OsERF52 protein is shown in SEQ ID No. 2. The present invention clarifies for the first time that the rice OsERF52 gene is involved in low temperature response at the protein level, and its protein content gradually increases with low temperature treatment; cold tolerance analysis shows that OsERF52 overexpressing rice plants have a low temperature resistance phenotype and a high survival rate, while Oserf52 mutants all show a low temperature sensitive phenotype, and the survival rate is significantly lower than that of the wild type, indicating that OsERF52 is a positive regulatory factor in rice response to low temperature. The rice OsERF52 protein and its encoding gene can be used to improve plant low temperature tolerance, which is of great significance for the study of the molecular mechanism of low temperature stress and the breeding of new low temperature resistant varieties.

The invention discloses a rice-crab symbiotic intelligent bait casting system and method based on water quality online monitoring, particularly relates to the technical field of fry and fingerling breeding, and aims to solve the problems that in the prior art, due to the fact that fixed point static water quality monitoring is depended on, dissolved oxygen space-time heterogeneity distribution in a rice-crab symbiotic system cannot be effectively adapted; the feeding decision is disjointed with the actual living environment and the feeding demand of the fish fries and the crab species; the method comprises the following steps: acquiring dissolved oxygen concentration monitoring data of a culture water body and growth state data of rice plants, judging spatial-temporal heterogeneity of dissolved oxygen based on dissolved oxygen data, and when heterogeneity exists, analyzing a plant shading mode and identifying a dynamic hypoxia risk area in combination with a fry gathering behavior. Meanwhile, a bottom-layer hypoxia risk area is evaluated based on benthic crab activity intensity distribution and sediment thickness, then the environment suitability of a candidate feeding area is evaluated by combining real-time dissolved oxygen data, and finally, the feeding position and the feeding amount are dynamically adjusted according to the suitability result.

The invention relates to a method and system for predicting the degree of rice bacterial blight disease based on multiple phenotypic parameters, and relates to the technical field of rice bacterial blight disease degree prediction. The method comprises firstly calculating the rice spectral reflectance corresponding to each plot in a study area based on a multispectral image, and screening characteristic variables based on the chlorophyll content of rice leaves and the water content of rice plants under the stress of rice bacterial blight, and establishing a new spectral index; secondly predicting the chlorophyll content of rice leaves and the water content of rice plants in the study area based on the rice spectral reflectance and a regression model, and then predicting the degree of rice bacterial blight; and then obtaining a rapid indication of the degree of rice bacterial blight disease based on the new spectral index and the prediction, and is suitable for high-throughput rice disease phenotypic monitoring research.

The invention relates to a rice transcription factor OsMTA1 and application of the rice transcription factor OsMTA1 in resisting rice viruses, and after an overexpression OsMTA1 vector is transferred into plant rice, a transgenic plant with stable inheritance is obtained. The result shows that compared with the control Nipponbare rice, the transgenic plant shows higher disease resistance.

This invention discloses a method and system for monitoring rice diseases and pests, relating to the field of disease and pest monitoring technology. First, the invention tests the resistance of different rice varieties to different diseases and pests. Then, it selects the optimal rice variety for the planting area, choosing varieties with strong resistance based on the local disease and pest situation to reduce disease and pest damage during later growth stages. This also reduces the cost of disease and pest monitoring, thereby lowering the overall cost of rice cultivation and laying the foundation for high-yield and high-quality rice cultivation. During rice cultivation, images of each individual rice plant are monitored, and a three-dimensional model is constructed to obtain the defense effect of each part of each individual plant. Abnormal parts and types of abnormal diseases and pests are then identified, providing a reference for subsequent disease and pest control, accurately locating the treatment site, and improving the effectiveness of disease and pest monitoring and control.

The utility model discloses a kind of rice herbicide spraying devices, belong to spraying device field, including rack, sliding block, guide light axis, fish eye bearing, the inside installation of rack has adjusting unit, spraying mechanism, the adjusting unit includes motor, two-way screw rod, bearing seat, adjusting sliding block, limiting nut, pin shaft, one side installation of motor has two-way screw rod, two-way screw rod is installed between two bearing seats, the surface of two-way screw rod is connected with adjusting sliding block and limiting nut, the inside installation of adjusting sliding block has pin shaft, the spraying mechanism includes main cross bar, nozzle, rectangular clamping groove, the surface of main cross bar is equidistant and has been opened with multiple groups of cylindrical groove, nozzle is installed in cylindrical groove, the surface of nozzle is connected with rectangular clamping groove;Through the cooperation between above each device, different rice varieties, different growth stages of row distance change can be quickly adapted, ensure that herbicide is accurately sprayed in rice row, reduce the drug damage to rice plant, improve weeding effect.

The invention belongs to the technical field of plant protection, discloses application of 4-hydroxycinnamyl aldehyde in inhibiting plant fungal pathogenic bacteria and application of 4-hydroxycinnamyl aldehyde in preventing and treating plant fungal diseases, and further discloses a product for inhibiting the plant fungal pathogenic bacteria and a product for preventing and treating the plant fungal diseases. Starting from the response of plants to pathogenic bacteria, the metabolite 4-hydroxycinnamyl aldehyde with obvious expression up-regulation in rice plants is detected, the metabolite 4-hydroxycinnamyl aldehyde is also a compound generated by an immune system after the plants cope with external pathogenic bacteria infection, and the research proves that the metabolite 4-hydroxycinnamyl aldehyde has an inhibition effect on various plant pathogenic bacteria, belongs to a broad-spectrum inhibitor, and can be used for preparing a compound for preventing and treating various plant pathogenic bacteria. There is no agent registered with the component in botanical pesticides registered at present, and the invention has important significance for development of environment-friendly novel botanical pesticides or synthesis of lead compounds, and provides technical support for large-scale production and large-area application of the botanical pesticides.

The invention discloses a gene type3AF for regulating and controlling the resistance of rice sheath blight disease as well as an encoding protein and application of the gene type3AF. The CDS sequence of the gene type3AF is as shown in SEQ ID NO.1, the full-length sequence of the gene type3AF is as shown in SEQ ID NO.2, and the protein sequence coded by the gene type3AF for regulating and controlling the rice sheath blight disease resistance is as shown in SEQ ID NO.3. According to the invention, a new rice sheath blight disease resistance negative regulation gene type3AF is successfully identified and obtained; two different banded sclerotial blight resistance identification methods verify that the knockout line of the type3AF gene in a rice plant can significantly improve the banded sclerotial blight resistance of the plant, and has no significant influence on main agronomic traits; however, overexpression of the type3AF gene in rice does not change the sheath blight resistance, but leads to significant changes in rice plant height and flag leaf length, and the type3AF gene has the research of breeding application value. The type3AF gene provided by the invention can provide a new resource for excavation of sheath blight resistant rice genes, provides a core target for cultivation of sheath blight resistant rice varieties, and has an important application value for maintenance of grain production safety.