97 results about "Rice plants" patented technology

The invention relates to the technical field of biology, in particular to application of an OgRKa gene and an upstream promoter thereof to improvement of rice bacterial blight and rice blast resistance, nucleotide sequences of the OgRKa gene and the upstream promoter are as shown in SEQ ID No.1, a CDS sequence is as shown in SEQ ID No.4, and an amino acid sequence of an encoding protein OgRKa is as shown in SEQ ID No.5. The homologous gene OgRKa of the rice MRKa gene (a rice plant over-expressing the MRKa gene has partial resistance to the rice bacterial blight, and a rice plant expressing the MRKa gene by using an MRKa self promoter has no resistance to the rice bacterial blight) is homologous cloned from oryza meyeriana through PCR (Polymerase Chain Reaction) amplification. Compared with the prior art, the resistance of rice plants with the OgRKa gene overexpressed by the 35S promoter or the OgRKa gene expressed by the promoter per se to a plurality of xanthomonas oryzae and magnaporthe oryzae is remarkably enhanced, the approximate immune level is achieved, the broad-spectrum high-resistance xanthomonas oryzae and magnaporthe oryzae are shown, and good application prospects are achieved in the aspect of breeding of xanthomonas oryzae and magnaporthe oryzae resistant varieties.

The invention relates to the field of ustilaginoidea virens prevention and treatment, and discloses a novel green ustilaginoidea virens prevention and treatment method which comprises the following steps: S1, irradiating seeds through a sterilization waveband ultraviolet lamp; s2, spraying the biological agent to rice plants in the booting stage of the rice; s3, selecting matrine plant extract to spray rice plants at a key stage when the rice is close to a crevasse and is about to enter a susceptible disease; s4, applying the electrolyzed water generator to prepare weakly acidic electrolyzed water in the heading stage of the rice, and spraying the weakly acidic electrolyzed water to the rice plants. Green prevention and control means such as ultraviolet sterilization, biological agents, plant extracts and electrolyzed water are adopted, the base number of pathogenic bacteria is reduced by treating seeds through ultraviolet rays, chemical residues are not introduced, bacillus subtilis serves as the biological agents, bacteria are used for bacteriostasis, matrine plant extracts are natural, and the environment-friendly effect is achieved. The weakly acidic and weakly alkaline electrolyzed water prevents and controls diseases by virtue of unique physicochemical properties, and is pollution-free.

Herbicide-resistant rice plants, isolated polynucleotides that encode herbicide resistant and wild-type acetohydroxy-acid synthase large subunit 1 (AHASL1) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to imidazolinone herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.

The invention discloses bacillus velezensis as well as a biological preparation and application thereof, and belongs to the technical field of microorganisms. The bacillus velezensis is rice endophytic bacillus velezensis, is classified and named as bacillus velezensis HA-B13, is preserved in the China Center for Type Culture Collection (CCTCC), has the preservation number of CCTCC NO: M 20251098, shows extremely excellent biological activity to nigatosporium oryzae, has the antibacterial rate of 94%, and can be used for preparing the bacillus velezensis. Meanwhile, the bacillus subtilis also has relatively good biological activity on a plurality of rice pathogenic bacteria such as rhizoctonia solani, pyricularia grisea, gylastoma gonoryzae and the like. And the strain is derived from rice plants and can be well colonized in the rice plants, so that the strain has a long-acting prevention and treatment effect on diseases such as spike rot and the like, can effectively reduce the investment of rice disease prevention and treatment products, is safer and has great prevention and treatment application potential.

The invention discloses application of a rice zinc finger protein OsRFI2 and a coding gene thereof in disease-resistant breeding, and belongs to the field of gene engineering. The rice zinc finger protein OsRFI2 is identified, the amino acid sequence of the rice zinc finger protein OsRFI2 is as shown in SEQ ID NO.2, and the nucleotide sequence of the coding gene OsRFI2 is as shown in SEQ ID NO.1. According to the invention, Zhonghua-11 rice is taken as a background, a knockout plant and an overexpression plant of the OsRFI2 gene are constructed, inoculation experiment results show that the scab length of the overexpression plant of the OsRFI2 gene is significantly smaller than that of the background plant, the overexpression of the OsRFI2 gene significantly improves the resistance of the rice plant to rice blast, and the rice zinc finger protein OsRFI2 positively regulates the disease resistance of the plant. The invention provides a new breeding strategy and a new breeding material for rice disease-resistant breeding, and has great economic benefits and wide application prospects for rice production.

The invention provides a method for regulating and controlling the heavy metal content of rice through MORC6s and application, and particularly belongs to the technical field of rice cultivation. The invention provides a gene for regulating and controlling the heavy metal content of the root part and the overground part of rice. The gene comprises any one or more of MORC6a, MORC6b and MORC6c. The MORC6a gene, the MORC6b gene and the MORC6c gene are subjected to induced expression after being treated by the heavy metal cadmium for different times. The invention finds that the MORC6a, MORC6b and MORC6c genes play an important role in regulating and controlling the accumulation process of cadmium at the root part and the overground part of rice. By utilizing the MORC6a, MORC6b and MORC6c genes, the harm of soil cadmium pollution to the growth and development of rice plants can be relieved, the food safety problem caused by excessive cadmium content is relieved, effective reference is further provided for low-cadmium rice cultivation, and accumulation of genetic resources of the low-cadmium rice is enriched.

This invention discloses a cultivated rice from Africa pi- cnv9 The application of genes in improving rice disease resistance belongs to the field of genetic engineering technology. This invention has screened a gene associated with rice blast resistance. pi-cnv9 The gene, whose nucleotide sequence is shown in SEQ ID NO.1, was used in this invention to construct complementary transgenes for *Rhizophora flavescens* and *Rhizophora esculenta*, respectively. pi-cnv9 Rice plants with complementary genes. Inoculation experiments were conducted on wild-type and transgenic plants, and the results demonstrated that complementary transgenic... pi-cnv9 Genetically modified plants are more resistant to disease than wild-type plants; indicating that... pi-cnv9 Genes that positively regulate the disease resistance of rice can be used as target genes for genetic improvement and molecular breeding of rice resistance to rice blast, which is of great significance for breeding rice varieties with improved disease resistance.

The application discloses a method for preparing rice with changed tiller number, and belongs to the technical field of genetic breeding. The method comprises the following steps: introducing a coding gene of an OsD14 mutant into target rice to obtain rice with changed tiller number, wherein the tiller number of the rice with changed tiller number is higher or lower than that of the target rice, and the OsD14 mutant is a protein obtained by mutating the 10th, 11th, 13th and 15th amino acids in SEQ ID NO: 1 into other amino acids and keeping other amino acids in SEQ ID NO: 1 unchanged. It is proved by experiments that whether ubiquitination degradation of the OsD14 occurs is related to a phosphorylation state of the OsD14, the number of rice tillers is adjusted by using the phosphorylation state of the OsD14, and the plant type is improved, so that the yield of the rice is improved, and the method has a wide application prospect.

The invention discloses application of an ELSA gene to improvement of rice disease resistance. Researches show that knockout of an ELSA gene promoter region or a gene body region can improve the resistance of rice to bacterial leaf blight, bacterial streak and rice blast, has no significant influence on the plant type, panicle type and the like of rice, and maintains the stability of the rice yield, that is, the rice yield is not influenced. It is indicated that the ELSA gene is a negative regulation gene of rice bacterial leaf blight, bacterial streak and rice blast resistance. Therefore, the rice with high disease resistance and stable yield can be cultivated by knocking out the rice ELSA gene promoter region or gene body region, and a simple and effective method is provided for cultivating the rice with high disease resistance and stable yield.

The present invention discloses the use of the OsST1 gene in regulating rice salt tolerance. This invention belongs to the field of plant genetic engineering technology. The invention provides the use of the OsST1 gene in regulating rice salt tolerance, demonstrating for the first time the role of the rice OsST1 gene in rice salt tolerance. Research has shown that overexpressing the OsST1 gene can improve the salt tolerance of rice plants and their survival rate under salt stress, demonstrating that the OsST1 gene has a role in regulating plant salt tolerance and addresses the adverse effects of soil salinization on rice growth in practical applications. Furthermore, the verification of the gene's biological function is of great significance for research into the molecular mechanism of rice salt tolerance.

The present disclosure relates to a mutant sequence of rice OsABCD1 gene and a method and application thereof in regulating cadmium accumulation in rice. Specifically, it relates to a mutant sequence of rice OsABCD1 gene, a protein encoded by the mutant sequence, a vector, a transformant, a method for reducing cadmium accumulation in rice plants and the application thereof. The present disclosure makes the function of the gene lost by gene mutation at the target site I of rice OsABCD1 gene, thereby reducing the accumulation of cadmium in rice; provides gene resources and technical support for breeding rice varieties with reduced cadmium absorption content, effectively improves the problem of excessive cadmium content in rice, and has important agricultural application value.

The invention provides an OsWRKY19 gene and an application of the OsWRKY19 gene in resistance to cochliobolus miyabeanus of rice. A CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 (CRISPR associated protein 9) technology is adopted to obtain an OsWRKY19 knockout mutant (Oswrky19) and an overexpression strain of And after the cochliobolus miyabeanus pathogenic bacteria are inoculated, compared with a wild type, the knockout mutant is more susceptible to diseases, and the over-expression strain resistance is obviously enhanced. The OsWRKY19 overexpression obviously improves the callose deposition level after rice plants are induced by chitin, and further enhances the basic defensive reaction of rice. RT-qPCR discovers that the expression of the defensive reaction related gene OsMBL1 in a knockout mutant is reduced and is increased in an overexpression plant, which indicates that the OsWRKY19 can promote the expression of the OsMBL1, thereby enhancing the resistance of rice. In conclusion, the OsWRKY19 is a gene for positively regulating and controlling the resistance of the cochliobolus miyabeanus of the rice, and can promote the basic defense response of the rice and the expression of a disease-resistant related gene, so that the resistance of the rice to the cochliobolus miyabeanus is improved. The content of the invention provides important genetic resources and theoretical guidance for the breeding of the cochliobolus miyabeanus resistant variety of rice.

The invention discloses application of a nano-material composite plant growth regulator to improvement of rice stress resistance and inhibition of magnaporthe oryzae. The plant growth regulator is prepared from magnesium ions and a nano material ZIF-8. The conditioning agent has the advantages that the conditioning agent can effectively inhibit the growth of magnaporthe oryzae hyphae and the formation of appressorium, and the morbidity of the magnaporthe oryzae can be remarkably reduced by mixing the conditioning agent with magnaporthe oryzae conidia to be sprayed and inoculated to rice leaves; meanwhile, the regulator can activate an anti-oxidation defense system of rice and enhance the stress resistance of plants. After the fertilizer is applied in a magnesium-deficient environment, key physiological indexes such as plant height, chlorophyll content, stomatal conductance, normalized difference vegetation index, maximum photochemical efficiency and the like of rice plants can be remarkably improved, so that the growth state of the rice plants is recovered to a normal level. Biosafety verifies that the regulator is non-toxic and environment-friendly to rice, and pollution caused by traditional chemical pesticides can be reduced.

The invention provides a DSE chlamydospore water aqua as well as a preparation method and application thereof, and belongs to the technical field of microbial preparations, the DSE chlamydospore water aqua comprises chlamydospore from at least one dark partitioned endophytic fungus, and further comprises 5g / mL-10g / mL of preservative and 5g / mL-10g / mL of protective agent, and the content of the chlamydospore is greater than 2.5 * 10 < 8 > CFU / mL. The product property of the water aqua is improved by reasonably optimizing the dosage of DSE chlamydospore and auxiliary materials, the content of chlamydospore in the obtained water aqua finished product can reach 2.54 * 10 < 8 > CFU / mL, and all indexes meet the national standard; the conidium water aqua can be stored for more than 12 months at normal temperature, and the shelf life is prolonged by more than 9 months compared with a conventional conidium water aqua. The DSE chlamydospore aqueous solution can effectively prevent and treat tomato bacterial wilt and southern rice black-streaked dwarf disease, improve the growth vigor of tomato plants and rice plants, and achieve a prevention and treatment effect which is not inferior to or even superior to that of positive control trichoderma harzianum. The DSE chlamydospore aqueous solution provided by the invention is simple to prepare, controllable in cost and excellent in performance, and is suitable for large-scale popularization and application as a novel biopesticide product.

This invention discloses a method and microbial preparation for controlling mercury accumulation in rice grains using Metarhizium anisopliae colonization, belonging to the field of agricultural environment and microbiology technology. The method involves inoculating rice plants with Metarhizium anisopliae spores, followed by planting the rice in mercury-contaminated paddy fields; water management is implemented throughout the rice's growth cycle until harvest. This invention establishes a complete microbial remediation system for mercury contamination in paddy fields, providing not only a method for the colonization and application of Metarhizium anisopliae in mercury-contaminated environments, but also ensuring safe rice production, offering a feasible approach to safeguarding food security and human health.

The present invention belongs to the field of molecular biology, and specifically discloses an application of a rice OsERF52 protein and its encoding gene in improving plant low temperature tolerance, and relates to the field of plant genetic engineering technology. The amino acid sequence of the rice OsERF52 protein is shown in SEQ ID No. 2. The present invention clarifies for the first time that the rice OsERF52 gene is involved in low temperature response at the protein level, and its protein content gradually increases with low temperature treatment; cold tolerance analysis shows that OsERF52 overexpressing rice plants have a low temperature resistance phenotype and a high survival rate, while Oserf52 mutants all show a low temperature sensitive phenotype, and the survival rate is significantly lower than that of the wild type, indicating that OsERF52 is a positive regulatory factor in rice response to low temperature. The rice OsERF52 protein and its encoding gene can be used to improve plant low temperature tolerance, which is of great significance for the study of the molecular mechanism of low temperature stress and the breeding of new low temperature resistant varieties.

The invention discloses a rice-crab symbiotic intelligent bait casting system and method based on water quality online monitoring, particularly relates to the technical field of fry and fingerling breeding, and aims to solve the problems that in the prior art, due to the fact that fixed point static water quality monitoring is depended on, dissolved oxygen space-time heterogeneity distribution in a rice-crab symbiotic system cannot be effectively adapted; the feeding decision is disjointed with the actual living environment and the feeding demand of the fish fries and the crab species; the method comprises the following steps: acquiring dissolved oxygen concentration monitoring data of a culture water body and growth state data of rice plants, judging spatial-temporal heterogeneity of dissolved oxygen based on dissolved oxygen data, and when heterogeneity exists, analyzing a plant shading mode and identifying a dynamic hypoxia risk area in combination with a fry gathering behavior. Meanwhile, a bottom-layer hypoxia risk area is evaluated based on benthic crab activity intensity distribution and sediment thickness, then the environment suitability of a candidate feeding area is evaluated by combining real-time dissolved oxygen data, and finally, the feeding position and the feeding amount are dynamically adjusted according to the suitability result.

The invention relates to a rice transcription factor OsMTA1 and application of the rice transcription factor OsMTA1 in resisting rice viruses, and after an overexpression OsMTA1 vector is transferred into plant rice, a transgenic plant with stable inheritance is obtained. The result shows that compared with the control Nipponbare rice, the transgenic plant shows higher disease resistance.

This invention discloses a method and system for monitoring rice diseases and pests, relating to the field of disease and pest monitoring technology. First, the invention tests the resistance of different rice varieties to different diseases and pests. Then, it selects the optimal rice variety for the planting area, choosing varieties with strong resistance based on the local disease and pest situation to reduce disease and pest damage during later growth stages. This also reduces the cost of disease and pest monitoring, thereby lowering the overall cost of rice cultivation and laying the foundation for high-yield and high-quality rice cultivation. During rice cultivation, images of each individual rice plant are monitored, and a three-dimensional model is constructed to obtain the defense effect of each part of each individual plant. Abnormal parts and types of abnormal diseases and pests are then identified, providing a reference for subsequent disease and pest control, accurately locating the treatment site, and improving the effectiveness of disease and pest monitoring and control.

The utility model discloses a kind of rice herbicide spraying devices, belong to spraying device field, including rack, sliding block, guide light axis, fish eye bearing, the inside installation of rack has adjusting unit, spraying mechanism, the adjusting unit includes motor, two-way screw rod, bearing seat, adjusting sliding block, limiting nut, pin shaft, one side installation of motor has two-way screw rod, two-way screw rod is installed between two bearing seats, the surface of two-way screw rod is connected with adjusting sliding block and limiting nut, the inside installation of adjusting sliding block has pin shaft, the spraying mechanism includes main cross bar, nozzle, rectangular clamping groove, the surface of main cross bar is equidistant and has been opened with multiple groups of cylindrical groove, nozzle is installed in cylindrical groove, the surface of nozzle is connected with rectangular clamping groove;Through the cooperation between above each device, different rice varieties, different growth stages of row distance change can be quickly adapted, ensure that herbicide is accurately sprayed in rice row, reduce the drug damage to rice plant, improve weeding effect.

The invention discloses a gene type3AF for regulating and controlling the resistance of rice sheath blight disease as well as an encoding protein and application of the gene type3AF. The CDS sequence of the gene type3AF is as shown in SEQ ID NO.1, the full-length sequence of the gene type3AF is as shown in SEQ ID NO.2, and the protein sequence coded by the gene type3AF for regulating and controlling the rice sheath blight disease resistance is as shown in SEQ ID NO.3. According to the invention, a new rice sheath blight disease resistance negative regulation gene type3AF is successfully identified and obtained; two different banded sclerotial blight resistance identification methods verify that the knockout line of the type3AF gene in a rice plant can significantly improve the banded sclerotial blight resistance of the plant, and has no significant influence on main agronomic traits; however, overexpression of the type3AF gene in rice does not change the sheath blight resistance, but leads to significant changes in rice plant height and flag leaf length, and the type3AF gene has the research of breeding application value. The type3AF gene provided by the invention can provide a new resource for excavation of sheath blight resistant rice genes, provides a core target for cultivation of sheath blight resistant rice varieties, and has an important application value for maintenance of grain production safety.

The invention relates to application of a rice OsbZIP34 gene in enhancing low-temperature stress resistance in a rice seedling stage, and belongs to the technical field of plant genetic engineering. Specifically, the rice OsbZIP34 gene participating in positive regulation and control of low-temperature stress resistance is obtained through cloning, an overexpression strain and a knockout line of the rice OsbZIP34 gene are constructed, and it is found that compared with a wild plant, the overexpression plant of the rice OsbZIP34 gene shows the characteristic of higher low-temperature resistance, the survival rate is remarkably increased, and the knockout line shows the opposite phenotype. In conclusion, the invention discloses a new function of the rice OsbZIP34 gene, and provides a new target and a new resource for genetic breeding for improving the low-temperature stress resistance of rice.

The invention relates to the technical field of plant genetic engineering, in particular to a mutant OsPBL8 gene, a preparation method thereof and application of the mutant OsPBL8 gene in enhancing rice disease resistance. The mutant OsPBL8 gene comprises an OsPBL8 gene mutant promoter full length and an OsPBL8 gene coding sequence full length, and the nucleotide sequence of the mutant OsPBL8 gene is shown as SEQ ID No: 5. The mutant OsPBL8 gene sequence is loaded on a pZ469 (pZCB11-1300-3xMyc-GFP) vector subjected to BamHI single enzyme digestion, then agrobacterium tumefaciens is transferred, and the gene is transfected into an initial rice plant by an agrobacterium tumefaciens-mediated transformation method to form transgenic rice. By modifying the promoter of the OsPBL8 gene, the disease resistance of the rice can be remarkably improved, so that a mutant OsPBL8 gene overexpression plant still has excellent rice blast resistance in an environment with a relatively large day and night temperature difference.

The present invention discloses a molecular marker linked to a major QTL regulating rice whiteback whitefly resistance. The QTL is located on rice chromosome 1, with a genetic distance of 30.88-34.32 cM and a physical distance of 7203302-8007653 bp. Molecular markers are then set on both sides of the QTL, and the molecular markers are used to screen rice varieties resistant to whiteback whiteflies, thereby improving the efficiency of screening for ideal rice plant types.

The invention belongs to the technical field of biology. More specifically, the invention relates to a homovanillic acid biosynthetic gene and an application thereof in resisting brown planthopper. The research finds that the over-expression of the LOCOs03g03720 gene or the LOCOs01g40870 gene in the rice can improve the resistance of the rice to the brown planthopper and can also improve the content of homovanillic acid in the rice, and after the LOCOs03g03720 gene or the LOCOs01g40870 gene is knocked out, the resistance of the rice plant to the brown planthopper is reduced, and the content of the homovanillic acid in the rice is also reduced. According to the invention, the LOCOs03g03720 gene or the LOCOs01g40870 gene is over-expressed in the rice through a gene engineering technology, so that the brown planthopper-resistant rice variety is created, germplasm resources are provided for breeding of the brown planthopper-resistant rice variety, and the application prospect and value are very good.

The invention discloses application of scopoletin in brown planthopper resistance of rice and a detection method. According to the application of scopoletin in preparation of biopesticides or plant insect-resistant agents for preventing and controlling brown planthoppers, the scopoletin directly acts on the brown planthoppers, and the survival rate, the weight gain, the egg laying amount and the number of yeast-like symbiotic fungi in the brown planthoppers are inhibited. According to the method for screening brown planthopper-resistant rice, the content of scopoletin in leaf sheath tissue of a rice plant is larger than or equal to 80 micrograms per gram of dry weight, and the survival rate of brown planthoppers is reduced by 60% within 7 days in an artificial insect inoculation experiment. According to the invention, direct association between scopoletin and brown planthopper resistance in rice is discovered for the first time, and a new metabolite-mediated insect resistance theory is provided; meanwhile, the composition acts on physiological, reproductive and symbiotic bacteria of pests, so that the drug resistance risk is reduced; the method is environment-friendly, natural in source and low in residue; the application range is wide, and biopesticide and insect-resistant breeding screening indexes can be developed; all the methods are successfully reproduced in a laboratory, and the method has an industrial transformation basis.

The utility model relates to the technical field of insect killing lamp, concretely is a kind of rice disease and insect pest control is used insect killing lamp: including base, the surface of base is fixedly connected with first support rod, the surface of first support rod is fixedly connected with total motor, the surface of first support rod is fixedly connected with screw rod seat, the side of screw rod seat is fixedly connected with screw rod motor.The utility model is provided with screw rod seat, screw rod motor, can make transmission screw rod rotation and then drive slider and first connecting rod longitudinal displacement, to drive circular insect killing frame to displace downwards in scrubbing box inside, the vertical pole of circular insect killing frame is provided with scraper ring, and the scraper ring will scrape off the insect carcass on circular insect killing frame and drop in scrubbing box inside when circular insect killing frame displaces downwards, and the bottom of scrubbing box is inverted conical, and insect carcass will be collected to the inside of storage box due to gravity, without manpower scrubbing insect carcass in process, just need manpower to remove insect carcass in storage box, save a lot of time and energy, improve efficiency.

The present invention discloses a glufosinate herbicide-resistant gene GLR6 of rice and its applications, relating to the field of biotechnology. The nucleotide sequence of the gene GLR6 is as follows: (1) as shown in SEQ ID No.1 or SEQ ID No.2; or (2) the nucleotide sequence of a mutant, allele or derivative generated by adding, substituting, inserting or deleting one or more nucleotides. The present invention also provides the encoded protein of the gene GLR6, a recombinant construct, a recombinant host cell, and a method and application for conferring glufosinate herbicide resistance to rice by using the gene GLR6. The beneficial effects of the present invention are as follows: in the allelic variation of GLR6 and the loss-of-function mutants obtained by gene editing, they have glufosinate herbicide resistance at the seedling stage. By using these mutant rice plants in direct seeding cultivation, glufosinate herbicide can be used to control weeds in the field, thereby quickly achieving the removal of weeds in the field.

The invention belongs to the technical field of leaf surface resistance and control agents, and particularly relates to a leaf surface resistance and control agent for reducing the cadmium content of rice and a preparation method thereof. The leaf surface blocking and controlling agent for reducing the cadmium content of the rice is formed by compounding active ingredients of oligosaccharide and oxaloacetic acid, and the mass ratio of the oligosaccharide to the oxaloacetic acid is (1-100): (100-1). Chitosan oligosaccharide and oxaloacetic acid are mixed to serve as an active component of the leaf surface blocking and controlling agent, and rice tests find that when the active component is sprayed on leaf surfaces of rice in the booting stage and the filling stage respectively, transportation and accumulation of heavy metal cadmium in soil in the rice can be effectively prevented and controlled, and then the content of the heavy metal cadmium in the rice can be reduced; and support can be provided for green and safe production of rice. In addition, positive ions in inorganic salts such as zinc sulfate, manganese sulfate and ferrous sulfate can antagonize transfer of cadmium and lead in rice plants to the rice, so that accumulation of heavy metal cadmium and lead in the rice is reduced, elements such as zinc, manganese and iron required by growth of the rice can be supplemented, and growth of the rice plants is promoted.

The invention provides a first season rice harvesting stubble height and ratooning rice yield prediction method, and relates to the technical field of agricultural planting. The method comprises four core modules including first rice variety whole growth period selection, first-season rice harvesting stubble height determination, second-crop rice effective ear calculation and ratooning rice yield prediction, wherein the whole growth period of the first rice variety is determined through a formula (1) and a formula (2) in combination with regional climate characteristics, and the influence of cold dew wind is avoided; a 6-9-point sampling method is adopted to observe the lengths of base nodes and inverted 1-4 nodes of rice plants and axillary bud vitality, empirical values of fixed heights are abandoned, and the heights of low stubbles, medium stubbles and high stubbles are scientifically divided; on the basis of the first rice effective ears, the axillary bud ear percentage and the harvester pressure loss correction rate, second-crop rice effective ears are calculated through a series of formulas (4); and predicting the yield of ratooning rice by using a series of formulas (5) in combination with parameters such as the grain setting rate and the maturing rate. The method realizes whole-process mechanized dry direct seeding and ratooning rice cultivation, can increase the annual yield per unit area, saves the cost, and is suitable for large-scale planting of ratooning rice.