38 results about "Appressorium" patented technology

The present invention is in the field of agricultural pathogen control. Methods and compositions are described for controlling plant diseases caused by pathogens, including fungi and oomycete phytopathogens. Chemicals were screened for activity as autophagy inhibitors. The screening method used a bioluminescence resonance energy transfer (BRET)-based high-throughput screening strategy to identify compounds that inhibit fungal ATG4 cysteine protease-mediated cleavage of ATG8 for autophagosome formation. Ebselen and ebselen analogs were identified as inhibitors of fungal pathogens ATG4-mediated ATG8 processing. Identified chemicals inhibit spore germination, hyphal development, and appressorium formation. The autophagy inhibitors significantly reduced the virulence of pathogens in their corresponding plant hosts. The invention also provides for compositions comprising the same, methods of making the same, and methods of controlling plant disease.

The invention discloses bacillus and application thereof, and belongs to the technical field of microorganisms, the bacillus is preserved in China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: M 20242290, and the preservation date is October 22, 2024. The composition can effectively inhibit vegetative growth and vegetative propagation of pathogenic bacteria such as colletotrichum gloeosporioides and paecilomyces japonicus, and has a good and broad-spectrum control effect on various pathogenic fungi. Bacteriostatic components contained in fermentation filtrate of the bacillus can remarkably inhibit germination of colletotrichum spruce spores and formation of appressorium. And the bacillus has a good prevention and treatment effect on the citrus huanglongbing.

The invention belongs to the technical field of prevention and treatment of rice blast, and particularly relates to a polypeptide MP1 for inhibiting rice blast bacteria and application thereof. The invention discloses a polypeptide MP1 for inhibiting magnaporthe oryzae. The amino acid sequence of the polypeptide MP1 is as shown in SEQ ID NO. 1. The polypeptide MP1 has an obvious inhibition effect on the magnaporthe oryzae appressorium, and the EC50 formed by the MP1 polypeptide on the appressorium is 134.60 mu M. Experiments show that the polypeptide can effectively interfere the normal differentiation and development of the appressorium in the key pathogenic stage of magnaporthe oryzae, so that the polypeptide has a strong prevention and control potential in the early stage of pathogenic bacterium infection. The polypeptide MP1 provided by the invention can significantly reduce the pathogenicity of magnaporthe oryzae to barley and rice, and provides an experimental basis for developing a novel biopesticide for preventing and treating the magnaporthe oryzae.

The invention provides a botanical fungicide of a chaulmoogra seed extract, which takes sub-chaulmoogra seed oleic acid as a marker, and application of the botanical fungicide in preparation of medicines for preventing and treating plant diseases caused by pathogenic fungi and oomycetes. The hydnocarpus anthelmintica extract is obtained by extracting hydnocarpus anthelmintica seeds with a plurality of different types of organic solvents such as ethanol, petroleum ether and the like and treating the seeds by a physical method, and the hydnocarpus anthelmintica extract contains sub-hydnocarpus anthelmintica oleic acid as markers in different proportions. The extract has the effects of inhibiting conidium germination, bud tube growth or appressorium formation of fungi and oomycetes, effectively preventing pathogenic bacteria from infecting host plants and preventing and treating important plant diseases such as rice blast, mango anthracnose, potato late blight and rice false smut.

The invention provides a rhodopseudomonas palustris antibacterial peptide, an antibacterial product and application, and belongs to the technical field of biological prevention and treatment medicine manufacturing. According to the application disclosed by the invention, two functional short peptides, namely KKKYG and VVGRR, with remarkable fungal antagonism activity are identified from extracellular polypeptide components of rhodopseudomonas palustris for the first time. Experiments prove that the two types of oligopeptides not only can effectively inhibit the formation of magnaporthe oryzae appressorium, but also can obviously weaken the pathogenicity of the oligopeptides on rice hosts and inhibit the pathogenicity of magnaporthe oryzae on rice, and have potential broad-spectrum antagonistic fungal activity and excellent biological control potential. The functional oligopeptide liquid preparation or freeze-dried powder prepared on the basis of the method is non-toxic to people and livestock, has no phytotoxicity to crops, does not pollute the environment, and meets the core requirements of green agriculture and sustainable development; and the oligopeptide preparation shows good heat-resistant stability, is convenient to store and transport, and adapts to complex and changeable field environments.

The application discloses an antibacterial peptide for preventing and treating rice blast and application thereof, and relates to an antibacterial peptide SAFM160 for preventing and treating rice blast, which is used for improving the resistance of rice to rice blast fungus, and can be used for preparing a medicine for resisting rice blast fungus and application in preventing and treating rice blast fungus infection. The application finds that the polypeptide SAFM160 can be used as a medicine for preventing and treating rice blast. The antibacterial peptide SAFM160 has obvious inhibiting effect on appressorium of rice blast fungus, and when the concentration of the SAFM160 is 0.2 ug / ul, the appressorium formation rate of spores of the rice blast fungus is 0.57%. Therefore, the antibacterial peptide SAFM160 can be used for improving the resistance of rice to rice blast fungus.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The invention discloses application of a nano-material composite plant growth regulator to improvement of rice stress resistance and inhibition of magnaporthe oryzae. The plant growth regulator is prepared from magnesium ions and a nano material ZIF-8. The conditioning agent has the advantages that the conditioning agent can effectively inhibit the growth of magnaporthe oryzae hyphae and the formation of appressorium, and the morbidity of the magnaporthe oryzae can be remarkably reduced by mixing the conditioning agent with magnaporthe oryzae conidia to be sprayed and inoculated to rice leaves; meanwhile, the regulator can activate an anti-oxidation defense system of rice and enhance the stress resistance of plants. After the fertilizer is applied in a magnesium-deficient environment, key physiological indexes such as plant height, chlorophyll content, stomatal conductance, normalized difference vegetation index, maximum photochemical efficiency and the like of rice plants can be remarkably improved, so that the growth state of the rice plants is recovered to a normal level. Biosafety verifies that the regulator is non-toxic and environment-friendly to rice, and pollution caused by traditional chemical pesticides can be reduced.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a polypeptide PP alpha for preventing and treating fungal diseases and application thereof. The invention discloses application of polypeptide PP alpha in inhibiting plant pathogenic fungi and preventing and treating plant diseases caused by the plant pathogenic fungi, the polypeptide PP alpha is a yeast alpha mating factor, and the amino acid sequence of the polypeptide PP alpha is shown as SEQ ID No.1. Experiments prove that the polypeptide PP alpha can significantly inhibit spore germination and appressorium formation of various plant pathogenic fungi such as pyricularia oryzae, cucurbitaceae colletotrichum spinosus, colletotrichum cryptum and botrytis cinerea, so that the infection process of the fungi is blocked. The polypeptide PP alpha provided by the invention has broad-spectrum antifungal activity, can be used as a novel green bactericide for preventing and treating fungal diseases of crops, and has a wide application prospect.

The application discloses a Higgins anthracnose pathogenic gene ChTHR1 and application thereof. The nucleotide sequence of the ChTHR1 gene is shown as SEQ ID NO:1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.3. The application determines that the pathogenic gene ChTHR1 has a key role in spore production, the rate of appressorium formation, mycelium biomass, ultraviolet resistance, melanin production and melanin accumulation of Higgins anthracnose, and can be used as a drug target for drug design and screening against Higgins anthracnose. Higgins anthracnose spreads between leaf blades of Brassica parachinensis and other cruciferous vegetables in Guangdong by means of conidia, and the conidia are eliminated or inhibited to prevent the prevalence of plant anthracnose. The ChTHR1 gene is used as a target for drug development, a drug for preventing and treating anthracnose caused by Higgins anthracnose and reducing the pathogenicity of plant Higgins anthracnose is screened, and the effect of the drug is evaluated.

The application discloses application of an effector protein MoUPE7 in regulating Magnaporthe oryzae pathogenicity. The application constructs a knock-out vector of the gene MoUPE7 and introduces the vector into Magnaporthe oryzae protoplast, analyzes conidial germination and pathogenicity of the obtained knock-out mutant, and finds that the knock-out of the gene MoUPE7 delays conidial germination and appressorium formation of Magnaporthe oryzae, increases the sensitivity to H2O2, and significantly reduces the pathogenicity to rice. That is, inhibiting the expression of the effector protein MoUPE7 can reduce the pathogenicity of Magnaporthe oryzae and reduce the influence of Magnaporthe oryzae on the yield and quality of rice, and the reagent capable of inhibiting the expression of the effector protein MoUPE7 can be used for the prevention and treatment of rice blast. The application not only enriches the pathogenic mechanism of Magnaporthe oryzae, but also is favorable to the development of green prevention and control products of rice blast.

The invention discloses an antibacterial peptide for preventing and treating rice blast and application of the antibacterial peptide, the antibacterial peptide for preventing and treating the rice blast is an antibacterial peptide SAFM160, and the antibacterial peptide SAFM160 is used for improving resistance of rice to rice blast bacteria, can also be used for preparing drugs for resisting the rice blast bacteria and is applied to prevention and treatment of rice blast bacteria infection. The research finds that the polypeptide SAFM160 can be used as a medicament for preventing and treating rice blast. The antibacterial peptide SAFM160 has an obvious inhibition effect on magnaporthe oryzae appressorium, and when the concentration of the SAFM160 is 0.2 mu g / mu L, the appressorium formation rate of magnaporthe oryzae spores is 0.57%. Therefore, the antibacterial peptide SAFM160 disclosed by the invention can be used for improving the resistance of the rice to the magnaporthe oryzae.

The invention provides application of a compound HLF1-11 in preparation of a medicine for preventing and controlling magnaporthe oryzae and a medicine for inhibiting spore germination of the magnaporthe oryzae, and further provides a pesticide and a method for preventing and controlling the magnaporthe oryzae. The compound HLF1-11 has an obvious inhibiting effect on development of magnaporthe oryzae appressorium, can effectively inhibit pathogenicity of magnaporthe oryzae spores and can prevent and treat magnaporthe oryzae, so that a foundation is laid for research and development of related prevention and control drugs.

The invention belongs to the technical field of pesticides, and particularly relates to application of cordycepin in preparation of a preparation for preventing and treating rice blast. According to the application disclosed by the invention, the cordycepin has the effects of inhibiting mycelial growth of magnaporthe oryzae, growth and development of spores and formation of appressorium and reducing pathogenicity of the spores for the first time, and the agricultural application of the cordycepin is expanded. The cordycepin as a biological agent effectively inhibits growth, development and pathogenicity of the magnaporthe oryzae, as the concentration of the cordycepin is increased, the sporulation quantity can be reduced by 90% or above at most, the appressorium is reduced by 75%, the teratogenesis rate of the appressorium is up to 4 times or above, and the pathogenicity of mycelia and spores of the magnaporthe oryzae is obviously inhibited. The cordyceps sinensis agent is used as an active component of the biological agent, has low toxicity to human and livestock, low residue and good environmental compatibility, and can become a sustainable, environment-friendly and non-toxic method for preventing and treating magnaporthe oryzae.

The present application belongs to the field of agricultural biotechnology, and through a large number of sequence analysis and tests, a short peptide with a function of antagonizing fungi in an extracellular protein Atp2 beta subunit protein of Rhodopseudomonas palustris is found. Tests show that the short peptide with the function of antagonizing fungi has low production cost, is non-toxic and environmentally friendly, can significantly inhibit the formation of appressorium of rice blast fungus and the pathogenicity on the host, and has potential broad-spectrum antagonistic function activity.

The invention discloses a drug target gene and an encoding protein MoRsc1 thereof, and relates to the field of plant genetic engineering. The method comprises the following steps: constructing a gene knockout vector, and introducing the gene knockout vector into a magnaporthe oryzae protoplast; the method comprises the following steps: knocking out a drug target gene RSC1 from magnaporthe oryzae by using a homologous recombination method to obtain a knockout mutant; the obtained magnaporthe oryzae knockout mutant has defects in the aspect of appressorium formation. A pathogenicity test shows that the toxicity of magnaporthe oryzae is remarkably reduced due to the deletion of the MoRSC1, and obvious disease spots cannot be formed on rice and barley leaves. The invention proves that MoRSC1 is necessary for the formation and pathogenicity of magnaporthe oryzae appressorium. According to the invention, the pathogenic molecular mechanism of magnaporthe oryzae can be deeply clarified, and a target gene is provided for the development of effective medicaments.

The application discloses application of a nano pesticide in inhibiting formation of appressorium of Pyricularia oryzae, wherein the nano pesticide is composed of copper ions and nano ZIF-8, and the copper ions are doped on the nano ZIF-8; wherein the mass ratio of the copper ions in the nano pesticide is 4.06 wt%. The nano pesticide has the advantages that not only can the formation of the appressorium of Pyricularia oryzae be inhibited, but also the production of active oxygen in mycelium can be induced, so that the morphological structure of the mycelium is caused to be oxidatively damaged; the nano pesticide mixed with conidia of Pyricularia oryzae is sprayed to inoculate rice leaves, the incidence of Pyricularia oryzae on the rice can be significantly reduced, and the prepared nano pesticide has no toxicity to the rice; Cu 2+ The copper ions doped into ZIF-8 and applied in inhibiting the formation of the appressorium of Pyricularia oryzae can not only improve the antibacterial activity of ZIF-8 on Pyricularia oryzae, but also reduce the pollution of traditional chemical pesticides to the environment.

The present application relates to substituted 1,2,3-triazole compounds represented by formula (I) and methods for their preparation, to pesticidal compositions comprising them and to their use in the control of plant diseases; in particular to substituted 1,2,3-triazole compounds capable of inhibiting fungal and oomycete appressorium formation and methods for their preparation and use in the control of plant diseases caused by fungi and oomycetes.

The present invention discloses the use of spiracoside in improving rice resistance to rice blast and / or sheath blight, and relates to the field of biotechnology. The present invention explores endogenous metabolites that can affect rice resistance to rice blast, and identifies the effects of endogenous metabolites on rice disease resistance in the field through three treatment methods: simultaneous treatment, preventive treatment, and therapeutic treatment. The results show that spiracoside can significantly enhance rice resistance to rice blast. The results show that after spiracoside treatment, the germination of rice blast fungus spores and the formation of appressorium are inhibited. In addition, the present invention also finds that spiracoside has preventive and therapeutic effects on rice sheath blight. The present invention provides a new endogenous metabolite for preventing and controlling rice blast and sheath blight, and has important agricultural application value.

The invention belongs to the field of biological genetic engineering, and particularly relates to a magnaporthe oryzae MoGFD1 gene and application thereof. The full-length sequence of the magnaporthe oryzae MoGFD1 gene is as shown in SEQ ID No. 1, the cDNA sequence is as shown in SEQ ID No. 2, and the amino acid sequence of the encoded protein is as shown in SEQ ID No. 3. The magnaporthe oryzae MoGFD1 gene is applied to regulation and control of growth and development of magnaporthe oryzae and reduction of pathogenicity of magnaporthe oryzae. By verifying physiological functions of the magnaporthe oryzae MoGFD1 gene in nutrition hypha growth, conidium production, appressorium formation, pathogenicity and the like, it is clear that the growth, development and pathogenicity of magnaporthe oryzae can be remarkably inhibited by replacing the magnaporthe oryzae MoGFD1 gene with a hygromycin resistance gene, and it is indicated that MoGFD1 is an important gene in the magnaporthe oryzae pathogenicity process. The MoGFD1 gene and the application thereof provided by the invention provide a new choice for prevention and control of rice blast.

This invention belongs to the field of rice blast disease control technology, specifically relating to a polypeptide MP1 that inhibits rice blast fungus and its application. This invention discloses a polypeptide MP1 that inhibits rice blast fungus, the amino acid sequence of which is shown in SEQ ID NO.1. This polypeptide MP1 has a significant inhibitory effect on rice blast fungus appressoriums; after 24 h, the MP1 polypeptide significantly inhibits the formation of ECs on appressoriums. 50 The concentration was 134.60 μM. Experiments showed that the peptide could effectively interfere with the normal differentiation and development of appressorium, a key stage in the pathogenesis of rice blast fungus, thus exerting strong control potential in the early stages of pathogen infection. The peptide MP1 provided by this invention can significantly reduce the pathogenicity of rice blast fungus to barley and rice, providing experimental evidence for the development of novel biological pesticides for the control of rice blast.

The invention discloses an application of an antibacterial peptide in preparation of a medicine for preventing and treating rice blast. The antibacterial peptide is 6-D-arginine. Research finds that 6-D-arginine can be used as a medicament for preventing and treating rice blast. The 6-D-arginine has an obvious inhibition effect on the formation of magnaporthe oryzae appressorium and the growth of mycelia. Therefore, the 6-D-arginine can be used for improving the resistance of the rice to the rice blast.

The invention relates to application of streptomyces mnorhizosphere and fermentation liquor thereof in inhibition of colletotrichum truncatum resisting picolinamide, and belongs to the technical field of biological prevention and control of crop diseases. The biocontrol strain 14-3 is named as streptomyces farnesii, is preserved in the Guangdong Microbial Culture Collection Center, and has the preservation number of GDMCC No. 63693. The strain shows remarkable antagonistic activity on colletotrichum truncatum with picolinamide resistance, and fermentation liquor of the strain can effectively inhibit mycelial growth of pathogenic bacteria, germination of conidia and formation of attachment spores and can cause spore splitting decomposition. In addition, fermentation liquor of the strain has a good prevention and treatment effect on soybean anthracnose caused by infection of colletotrichum truncatum resisting picolinamide, and shows good application potential in the aspect of crop drug resistance treatment.

The invention provides a thiophene boric acid ester compound with a novel structure. The compound has a remarkable inhibition effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as pyricularia oryzae, colletotrichum, gibberellic disease bacteria, phytophthora and phytophthora capsici).

The invention provides a siRNA nucleic acid pesticide for controlling magnaporthe oryzae by targeting a DICER2 gene, and relates to the technical field of biology. The siRNA molecule can delay and inhibit the formation of magnaporthe oryzae appressorium, so that the pathogenicity of magnaporthe oryzae is reduced, and a new method for preventing and treating magnaporthe oryzae is provided; when the composition is combined with tricyclazole or clotrimazole for use, a synergistic effect is achieved. The siRNA molecule of the targeted DICER2 gene is prepared into a nucleic acid pesticide, and the nucleic acid pesticide is sprayed on leaves of rice, so that leaf rice blast scab and scab area are effectively reduced, the siRNA molecule can be further used for preventing and treating rice blast, and the tedious process of cultivating transgenic crops and the worry of consumer groups can be avoided.

The application discloses genes MoUPE12 and application thereof in reducing pathogenicity of Magnaporthe oryzae. The application obtains corresponding knockout mutants by constructing a knockout vector of genes MoUPE12 and introducing the knockout vector into Magnaporthe oryzae protoplasts. It is found through determination of conidium germination, appressorium development and pathogenicity to rice of the obtained knockout mutants that the conidium germination and appressorium development of the knockout mutants are slowed down, the glycogen metabolism of the conidia is slowed down, and the pathogenicity to rice is significantly reduced. After the gene MoUPE12 is complemented, the pathogenicity of the complemented mutant restores to the wild type level. That is, inhibition of expression of the gene MoUPE12 can reduce the pathogenicity of Magnaporthe oryzae, and the gene can be used as a target to develop Magnaporthe oryzae prevention and treatment products. The application enriches the pathogenic mechanism of Magnaporthe oryzae and is beneficial to development of Magnaporthe oryzae prevention and treatment products.

The invention discloses application of a kadsura coccinea extract in preparation of a bactericide for preventing and treating plant diseases caused by pathogenic fungi and oomycetes. The kadsura coccinea extract disclosed by the invention has the effects of inhibiting germination of conidia of fungi and oomycetes, growth of bud tubes or formation of appressorium and growth of nutritional hyphae, and can effectively prevent growth of pathogenic bacteria and infection of host plants; the bactericidal composition has certain prevention and treatment effects on rice blast, rice false smut, potato late blight, cucumber anthracnose, grape anthracnose, corn ear rot, pepper phytophthora blight and camphor tree phytophthora blight, and provides possibility for developing botanical bactericides with various effective components, efficient prevention and treatment effects and broad-spectrum prevention and treatment objects.

The invention discloses a drug screening chip based on Pcg6 protein, a method and application thereof, and belongs to the field of biotechnology and pesticide screening. The chip comprises a solid-phase carrier and Pcg6 protein, a variant thereof, an ortholog thereof or a functional active fragment thereof which are fixed on the surface of the solid-phase carrier. The invention also discloses a method for screening a plant pathogenic fungus (such as magnaporthe oryzae) small molecule compound by using the protein or the chip. The method relates to technical means such as surface plasma resonance (SPR), DNA coding compound library (DEL) screening, affinity mass spectrometry or virtual docking and the like. According to the invention, a benzothiophene small molecule compound PN-16 having physical interaction with Pcg6 can be screened by detecting the binding condition of a candidate compound and Pcg6 protein; by measuring the formation rate of appressorium and inoculating barley and rice, the PN-16 is determined to have relatively high activity.

The invention provides screening and application of siRNA of a magnaporthe oryzae MOHS1 gene, and relates to the technical field of biology. The siRNA is used for carrying out targeted silence on the MOHS1 gene (MGG08523) of the magnaporthe oryzae; the siRNA molecule can delay and inhibit the formation of magnaporthe oryzae appressorium, so that the pathogenicity of magnaporthe oryzae is reduced, and a new method for preventing and treating magnaporthe oryzae is provided. The siRNA molecule of the targeted MOHS1 gene is prepared into a nucleic acid pesticide, and the nucleic acid pesticide is sprayed on leaves of rice, so that leaf rice blast scab and scab area are effectively reduced, the siRNA molecule can be further used for preventing and treating rice blast, and the tedious process of cultivating transgenic crops and the worry of consumer groups can be avoided.

The invention discloses a gene MoUPE12 and application thereof in reducing pathogenicity of magnaporthe oryzae. A corresponding knockout mutant is obtained by constructing a knockout vector of the gene MoUPE12 and introducing a magnaporthe oryzae protoplast. The determination of the conidia germination and appressorium development conditions of the obtained knockout mutant and the pathogenicity to rice finds that the conidia germination and appressorium development of the obtained knockout mutant are slowed down, the glycogen metabolism of the conidia is slowed down, and the pathogenicity to the rice is obviously reduced. And after the gene MoUPE12 is complemented, the pathogenicity of the complemented mutant is recovered to a wild type level. The pathogenicity of the pyricularia oryzae can be reduced by inhibiting expression of the gene MoUPE12, and the gene can be used as a target to develop pyricularia oryzae control products. The invention enriches the pathogenesis of magnaporthe oryzae and is beneficial to the development of magnaporthe oryzae prevention and