21 results about "Appressorium" patented technology

The invention belongs to the technical field of prevention and treatment of rice blast, and particularly relates to a polypeptide MP1 for inhibiting rice blast bacteria and application thereof. The invention discloses a polypeptide MP1 for inhibiting magnaporthe oryzae. The amino acid sequence of the polypeptide MP1 is as shown in SEQ ID NO. 1. The polypeptide MP1 has an obvious inhibition effect on the magnaporthe oryzae appressorium, and the EC50 formed by the MP1 polypeptide on the appressorium is 134.60 mu M. Experiments show that the polypeptide can effectively interfere the normal differentiation and development of the appressorium in the key pathogenic stage of magnaporthe oryzae, so that the polypeptide has a strong prevention and control potential in the early stage of pathogenic bacterium infection. The polypeptide MP1 provided by the invention can significantly reduce the pathogenicity of magnaporthe oryzae to barley and rice, and provides an experimental basis for developing a novel biopesticide for preventing and treating the magnaporthe oryzae.

The invention provides a rhodopseudomonas palustris antibacterial peptide, an antibacterial product and application, and belongs to the technical field of biological prevention and treatment medicine manufacturing. According to the application disclosed by the invention, two functional short peptides, namely KKKYG and VVGRR, with remarkable fungal antagonism activity are identified from extracellular polypeptide components of rhodopseudomonas palustris for the first time. Experiments prove that the two types of oligopeptides not only can effectively inhibit the formation of magnaporthe oryzae appressorium, but also can obviously weaken the pathogenicity of the oligopeptides on rice hosts and inhibit the pathogenicity of magnaporthe oryzae on rice, and have potential broad-spectrum antagonistic fungal activity and excellent biological control potential. The functional oligopeptide liquid preparation or freeze-dried powder prepared on the basis of the method is non-toxic to people and livestock, has no phytotoxicity to crops, does not pollute the environment, and meets the core requirements of green agriculture and sustainable development; and the oligopeptide preparation shows good heat-resistant stability, is convenient to store and transport, and adapts to complex and changeable field environments.

The application discloses an antibacterial peptide for preventing and treating rice blast and application thereof, and relates to an antibacterial peptide SAFM160 for preventing and treating rice blast, which is used for improving the resistance of rice to rice blast fungus, and can be used for preparing a medicine for resisting rice blast fungus and application in preventing and treating rice blast fungus infection. The application finds that the polypeptide SAFM160 can be used as a medicine for preventing and treating rice blast. The antibacterial peptide SAFM160 has obvious inhibiting effect on appressorium of rice blast fungus, and when the concentration of the SAFM160 is 0.2 ug / ul, the appressorium formation rate of spores of the rice blast fungus is 0.57%. Therefore, the antibacterial peptide SAFM160 can be used for improving the resistance of rice to rice blast fungus.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a polypeptide PP alpha for preventing and treating fungal diseases and application thereof. The invention discloses application of polypeptide PP alpha in inhibiting plant pathogenic fungi and preventing and treating plant diseases caused by the plant pathogenic fungi, the polypeptide PP alpha is a yeast alpha mating factor, and the amino acid sequence of the polypeptide PP alpha is shown as SEQ ID No.1. Experiments prove that the polypeptide PP alpha can significantly inhibit spore germination and appressorium formation of various plant pathogenic fungi such as pyricularia oryzae, cucurbitaceae colletotrichum spinosus, colletotrichum cryptum and botrytis cinerea, so that the infection process of the fungi is blocked. The polypeptide PP alpha provided by the invention has broad-spectrum antifungal activity, can be used as a novel green bactericide for preventing and treating fungal diseases of crops, and has a wide application prospect.

The application discloses a Higgins anthracnose pathogenic gene ChTHR1 and application thereof. The nucleotide sequence of the ChTHR1 gene is shown as SEQ ID NO:1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.3. The application determines that the pathogenic gene ChTHR1 has a key role in spore production, the rate of appressorium formation, mycelium biomass, ultraviolet resistance, melanin production and melanin accumulation of Higgins anthracnose, and can be used as a drug target for drug design and screening against Higgins anthracnose. Higgins anthracnose spreads between leaf blades of Brassica parachinensis and other cruciferous vegetables in Guangdong by means of conidia, and the conidia are eliminated or inhibited to prevent the prevalence of plant anthracnose. The ChTHR1 gene is used as a target for drug development, a drug for preventing and treating anthracnose caused by Higgins anthracnose and reducing the pathogenicity of plant Higgins anthracnose is screened, and the effect of the drug is evaluated.

The application discloses application of an effector protein MoUPE7 in regulating Magnaporthe oryzae pathogenicity. The application constructs a knock-out vector of the gene MoUPE7 and introduces the vector into Magnaporthe oryzae protoplast, analyzes conidial germination and pathogenicity of the obtained knock-out mutant, and finds that the knock-out of the gene MoUPE7 delays conidial germination and appressorium formation of Magnaporthe oryzae, increases the sensitivity to H2O2, and significantly reduces the pathogenicity to rice. That is, inhibiting the expression of the effector protein MoUPE7 can reduce the pathogenicity of Magnaporthe oryzae and reduce the influence of Magnaporthe oryzae on the yield and quality of rice, and the reagent capable of inhibiting the expression of the effector protein MoUPE7 can be used for the prevention and treatment of rice blast. The application not only enriches the pathogenic mechanism of Magnaporthe oryzae, but also is favorable to the development of green prevention and control products of rice blast.

The invention provides application of a compound HLF1-11 in preparation of a medicine for preventing and controlling magnaporthe oryzae and a medicine for inhibiting spore germination of the magnaporthe oryzae, and further provides a pesticide and a method for preventing and controlling the magnaporthe oryzae. The compound HLF1-11 has an obvious inhibiting effect on development of magnaporthe oryzae appressorium, can effectively inhibit pathogenicity of magnaporthe oryzae spores and can prevent and treat magnaporthe oryzae, so that a foundation is laid for research and development of related prevention and control drugs.

The present application belongs to the field of agricultural biotechnology, and through a large number of sequence analysis and tests, a short peptide with a function of antagonizing fungi in an extracellular protein Atp2 beta subunit protein of Rhodopseudomonas palustris is found. Tests show that the short peptide with the function of antagonizing fungi has low production cost, is non-toxic and environmentally friendly, can significantly inhibit the formation of appressorium of rice blast fungus and the pathogenicity on the host, and has potential broad-spectrum antagonistic function activity.

This invention belongs to the field of rice blast disease control technology, specifically relating to a polypeptide MP1 that inhibits rice blast fungus and its application. This invention discloses a polypeptide MP1 that inhibits rice blast fungus, the amino acid sequence of which is shown in SEQ ID NO.1. This polypeptide MP1 has a significant inhibitory effect on rice blast fungus appressoriums; after 24 h, the MP1 polypeptide significantly inhibits the formation of ECs on appressoriums. 50 The concentration was 134.60 μM. Experiments showed that the peptide could effectively interfere with the normal differentiation and development of appressorium, a key stage in the pathogenesis of rice blast fungus, thus exerting strong control potential in the early stages of pathogen infection. The peptide MP1 provided by this invention can significantly reduce the pathogenicity of rice blast fungus to barley and rice, providing experimental evidence for the development of novel biological pesticides for the control of rice blast.

The invention relates to application of streptomyces mnorhizosphere and fermentation liquor thereof in inhibition of colletotrichum truncatum resisting picolinamide, and belongs to the technical field of biological prevention and control of crop diseases. The biocontrol strain 14-3 is named as streptomyces farnesii, is preserved in the Guangdong Microbial Culture Collection Center, and has the preservation number of GDMCC No. 63693. The strain shows remarkable antagonistic activity on colletotrichum truncatum with picolinamide resistance, and fermentation liquor of the strain can effectively inhibit mycelial growth of pathogenic bacteria, germination of conidia and formation of attachment spores and can cause spore splitting decomposition. In addition, fermentation liquor of the strain has a good prevention and treatment effect on soybean anthracnose caused by infection of colletotrichum truncatum resisting picolinamide, and shows good application potential in the aspect of crop drug resistance treatment.

The invention provides a thiophene boric acid ester compound with a novel structure. The compound has a remarkable inhibition effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as pyricularia oryzae, colletotrichum, gibberellic disease bacteria, phytophthora and phytophthora capsici).

The application discloses genes MoUPE12 and application thereof in reducing pathogenicity of Magnaporthe oryzae. The application obtains corresponding knockout mutants by constructing a knockout vector of genes MoUPE12 and introducing the knockout vector into Magnaporthe oryzae protoplasts. It is found through determination of conidium germination, appressorium development and pathogenicity to rice of the obtained knockout mutants that the conidium germination and appressorium development of the knockout mutants are slowed down, the glycogen metabolism of the conidia is slowed down, and the pathogenicity to rice is significantly reduced. After the gene MoUPE12 is complemented, the pathogenicity of the complemented mutant restores to the wild type level. That is, inhibition of expression of the gene MoUPE12 can reduce the pathogenicity of Magnaporthe oryzae, and the gene can be used as a target to develop Magnaporthe oryzae prevention and treatment products. The application enriches the pathogenic mechanism of Magnaporthe oryzae and is beneficial to development of Magnaporthe oryzae prevention and treatment products.

The invention discloses application of a kadsura coccinea extract in preparation of a bactericide for preventing and treating plant diseases caused by pathogenic fungi and oomycetes. The kadsura coccinea extract disclosed by the invention has the effects of inhibiting germination of conidia of fungi and oomycetes, growth of bud tubes or formation of appressorium and growth of nutritional hyphae, and can effectively prevent growth of pathogenic bacteria and infection of host plants; the bactericidal composition has certain prevention and treatment effects on rice blast, rice false smut, potato late blight, cucumber anthracnose, grape anthracnose, corn ear rot, pepper phytophthora blight and camphor tree phytophthora blight, and provides possibility for developing botanical bactericides with various effective components, efficient prevention and treatment effects and broad-spectrum prevention and treatment objects.

The invention discloses a drug screening chip based on Pcg6 protein, a method and application thereof, and belongs to the field of biotechnology and pesticide screening. The chip comprises a solid-phase carrier and Pcg6 protein, a variant thereof, an ortholog thereof or a functional active fragment thereof which are fixed on the surface of the solid-phase carrier. The invention also discloses a method for screening a plant pathogenic fungus (such as magnaporthe oryzae) small molecule compound by using the protein or the chip. The method relates to technical means such as surface plasma resonance (SPR), DNA coding compound library (DEL) screening, affinity mass spectrometry or virtual docking and the like. According to the invention, a benzothiophene small molecule compound PN-16 having physical interaction with Pcg6 can be screened by detecting the binding condition of a candidate compound and Pcg6 protein; by measuring the formation rate of appressorium and inoculating barley and rice, the PN-16 is determined to have relatively high activity.

The application relates to the technical field of pesticides for rice, and aims to solve the problem of ecological environment damage caused by existing chemical pesticides, and provides application of Chymostatin in prevention and control of rice blast, including: S1, inhibiting rice Magnaporthe oryzae; S2, preventing and controlling rice blast; the CAS No of the Chymostatin is 9076-44-2. Chymostatin has a strong inhibiting effect on rice Magnaporthe oryzae, has low harm degree on animals such as human and livestock, is easy to degrade, is friendly to the environment, is beneficial to ecological balance and stability. Chymostatin can significantly inhibit the formation of Magnaporthe oryzae appressorium, and then inhibit the infection. Chymostatin has good inhibiting activity on various rice Magnaporthe oryzae and has broad spectrum.

This invention discloses the application of the rice OsSAFM6 gene or its encoded protein in enhancing rice resistance to rice blast fungus, relating to the field of rice blast control technology. Research by this invention has found that the protein encoded by the rice OsSAFM6 gene can be used as a pesticide to control rice blast. The OsSAFM6 protein has a significant inhibitory effect on the appressorium of rice blast fungus. When the SAFM6-GST concentration is 0.05 μg / μL, the appressorium formation rate of rice blast fungus spores is 26.67%; at a concentration of 0.1 μg / μL, the appressorium formation rate is 5.33%. Therefore, the protein encoded by the rice OsSAFM6 gene can be used to enhance rice resistance to rice blast fungus.

The invention discloses application of a magnaporthe oryzae squalene cyclooxygenase gene MoERG1 in prevention and treatment of magnaporthe oryzae, and belongs to the technical field of agricultural biology. The invention proves that the squalene cyclooxygenase gene MoERG1 of the magnaporthe oryzae is a key essential gene for environmental adaptability and pathogenicity of the magnaporthe oryzae. The gene is specifically silenced by an RNA interference technology, so that the completeness of cell walls of pathogenic bacteria can be effectively damaged, the tolerance of the pathogenic bacteria to oxidative stress and hypertonic stress is remarkably weakened, the yield of conidia and the formation rate of a key infection structure-appressorium of the pathogenic bacteria are greatly reduced, and finally, the pathogenic ability of the pathogenic bacteria to rice is completely lost. The core function of the squalene ring oxygenase gene MoERG1 as a multi-effect regulation target is disclosed from the molecular level. In order to solve the industrial problems that the existing single-target bactericide is easy to generate drug resistance and the traditional biocontrol effect is unstable, the invention provides a brand-new effective solution with a more fundamental action mechanism.

A siRNA targeting a key gene of Magnaporthe oryzae for controlling rice blast and use thereof, and relates to the field of biotechnology. The siRNA targets silence of a MOHS1 gene (MGG_08523) of Magnaporthe oryzae. The siRNA molecule can delay and inhibit the formation of an appressorium of the Magnaporthe oryzae, thereby reducing the pathogenicity of the Magnaporthe oryzae, thereby providing a novel method for controlling the rice blast. The siRNA molecule targeting the MOHS1 gene is prepared into a nucleic acid pesticide, and the nucleic acid pesticide is sprayed onto leaves of Oryza sativa L., which effectively reduces the number and area of lesions of the rice blast on the leaves, in turn can be used for controlling the rice blast, and can avoid a tedious process of cultivating genetically modified crops and the concerns of consumer groups.

The invention relates to the field of plant disease control, and discloses a siderophore antifungal agent and application thereof in plant disease control. The siderophore antifungal agent disclosed by the invention is prepared from the following components: (2R, 4R)-2-[(4R)-2-(2-hydroxyphenyl)-4, 5-dihydro-1, 3-thiazole-4-yl]-3-methyl-1, 3-thiazolidine-4-carboxylic acid. When the siderophore antifungal agent is used for preventing and treating plant diseases, the formation of conidium appressorium can be inhibited, and the proportion of anthrax hijinensis appressorium which intrudes hyphae and infects flowering cabbage cotyledons is only 6% and is obviously lower than 86% of a control group. The disease index of the flowering cabbage plant inoculated with the colletotrichum hijinensis can be obviously reduced from 74 to 27 at the concentration of 10 mu M, and 0 mm disease spots can be realized at the concentration of 30 mu M; meanwhile, the compound also has a certain control effect on fungi such as magnaporthe oryzae and colletotrichum gloeosporioides, and shows broad-spectrum antibacterial activity.

The invention provides a series of novel borate compounds as shown in a formula I and a preparation method thereof. The compound has the effects of remarkably inhibiting conidium germination, bud tube growth or appressorium formation of fungi and oomycetes, effectively preventing pathogenic bacteria from infecting host plants and preventing and treating important plant diseases such as rice blast, mango anthracnose, wheat scab, wheat powdery mildew, potato late blight and phytophthora capsici.