34 results about "Appressorium" patented technology

The invention discloses the function and the usage of a magnaporthe oryzae MoCHS1 gene and a coded protein thereof. The gene controls the conidium form, the conidium yield, the appressorium formation rate and the pathogenicity of magnaporthe oryzae. The gene as well as the cDNA and the coded protein thereof respectively comprise nucleotide or amino acid sequences SEQ ID No: 1, No: 2 and No: 3 in the sequence list. The knockout of the MoCHS1 gene causes the change of the form establishment of the magnaporthe oryzae conidium; the conidium is reduced and has no diaphragm or has only one diaphragm; the yield of the conidium is decreased to 2% of wild type bacterial strains; the formation rate of the appressorium is decreased to 60% of the wide type bacterial strains; and the infestation capability on rice vanes is obviously decreased. The protein coded by MoCHS1 or / and the expression or the modification of the homologous protein in other pathogenic fungi can be taken as important candidate targets and can be used for designing and screening novel antifungal drugs.

The invention discloses a specific promoter of pathogenic filamentous fungi. The nucleotide sequence of the specific promoter at least contains a DNA sequence represented by SEQ ID No.1, or a DNA sequence complementary to the sequence represented by the SEQ ID No.1, or a DNA sequence which is 90 or above familiar with the sequence represented by the SEQ ID No.1 or the complementary sequence of the sequence represented by the SEQ ID No.1 and has the same functions with the sequence represented by the SEQ ID No.1 or the complementary sequence of the sequence represented by the SEQ ID No.1. Experiment researches prove that the promoter can be specifically and effectively expressed in a period of appressorium. The promoter provides an important tool for studying the regulation of the pathogenic filamentous fungi in an appressorium formation and permeation into body wall of a host and reconstructing wild insect pathogenic strain gene engineering and has a very high application value.

The invention discloses a biocontrol bacillus subtilis BJ-1 and an application thereof. The bacillus subtilis is preserved in China Center for Type Culture Collection (CCTCC) with preservation number of CCTCC NO: M2017443. The bacillus subtilis provided by the invention, as a biocontrol bacterium, has a good inhibitory effect on various fungi. With the application of the bacillus subtilis BJ-1 provided by the invention, mycelial growth, spore germination and appressorium formation of rice blast can be effectively inhibited; as rice seeds are treated by being soaked in a solution prepared from the bacillus subtilis, rice growth can be promoted and plant resistance can be induced, and potential adverse influence of chemical control on environment and rice safety and infection risk in using of a disease-resistant variety can be prevented; and in addition, the bacillus subtilis, which is rapid in reproduction, quite strong in stability, high-temperature resistant and storage resistant and free from toxic and side effects, can be processed and developed in the dosage forms of viable fungicides and metabolites.

The invention discloses a new necessary MgPTH12 gene and utility to make pear spore bacterium attach to mature and lead to disease, which is characterized by the following: the gene and its cDNA and coding product possess the sequence in the SEQ ID No.:1, No.:2 and No.:3; the protein of coded gene possesses homeo structural region, which is not similar to known functional protein; the gene removes abnormal and matured attaching cell, which reduces forming frequency of infecting tin and extending infecting hypha without pathogenic ability for rice; the homologous gene of gene lies in the plant pathogenic bacteria widely, which can be applied to design and sieve new drug to prevent fungus.

The invention discloses a magnaporthe grisea MoPPF3 gene and function and application of coding protein of the magnaporthe grisea MoPPF3 gene. The gene can control formation and pathogenicity of a magnaporthe grisea infection nail, the gene and cDNA (complementary deoxyribonucleic acid) thereof and protein coded by the gene respectively have the nucleotide or amino acid sequence shown as SEQ ID No. 1, No. 2 and No. 3 in a sequence list. The gene can be expressed in conidium, appressorium and infected hypha of magnaporthe grisea and is necessary for fatty acid metabolism. The MoPPF3 gene is knocked out, so that formation rate of magnaporthe grisea is reduced to be 1 / 3 that of wild type, osmotic pressure endurance capacity is obviously reduced, and capability to infect rice leaves is completely lost. Expression or modification of the protein coded by the MoPPF3 gene or / and homogenous protein thereof in other pathogenic fungi can be taken as important candidate targets and can be used for designing and screening antifungal novel medicament. The invention discloses a magnaporthe grisea gene and function and application of the coding protein of the magnaporthe grisea MoPPF3 gene, wherein the gene can control formation and pathogenicity of the magnaporthe grisea infection nail.

The invention discloses a method for lowering sensitivity of a pyricularia grisea effect protein gene overexpression strain to pH. The method comprises the following steps: treating pyricularia grisea effect protein gene overexpression strain spores with pH5 and pH8; performing statistics to obtain spore yield, sprouting rate and appressorium forming rate. Compared with the prior art, the method has the advantages that the sensitivity of the pyricularia grisea effect protein gene overexpression strain to different pH can be easily, conveniently and accurately determined, and an important reference basis is laid for rapid comprehension of the pathogenicity of a pathogenic bacteria effect protein gene overexpression strain.

The invention relates to the technical field of biology, in particular to a downy mildew bacterium culture and observation method. The method comprises the following steps: collecting downy mildew bacteria; preparing sporangium suspension liquid; and culturing the sporangium suspensions. Through repeated tests, a technology capable of inducing sporangium of cucumber downy mildew to germinate andrelease zoospore is obtained, the zoospore forms static spores through movement and morphological changes, the static spores germinate to form appressorium, and the appressorium germinates to generateinfection filaments. The technology can provide an effective technique for researchers to deeply research downy mildew biological basis, disease resistance identification, bactericide screening and the like. By means of the method, researchers can achieve quantitative statistics and monitoring of the number of zoospore of downy mildew, morphological changes and the number of formed infectors, andresearch of the action mechanism of the bactericide can also be achieved. Several development stages which must be experienced before downy mildew bacteria invade a host are clearly observed. The systematic observation method can provide first-hand data for prevention and treatment of the pathogenic bacteria on symptomatic drugs.

The invention discloses a cunninghamia lanceolata Epicoccum dendrobii fermentation filtrate, an extract, a preparation method and an application thereof. The fermentation filtrate of the Epicoccum dendrobii SMEL disclosed by the invention can be used for remarkably inhibiting the colletotrichum gloeosporioides spore germination of the cunninghamia lanceolata and inhibiting the appressorium development of the colletotrichum gloeosporioides of the cunninghamia lanceolata; the ethyl acetate extract of the Epicoccum dendrobii SMEL fermentation filtrate can obviously inhibit the mycelial growth ofthe colletotrichum gloeosporioides of the cunninghamia lanceolata, and obviously inhibit the spore germination and appressorium development of the colletotrichum gloeosporioides of the cunninghamia lanceolata; and an inoculation test shows that the ethyl acetate extract obviously inhibits the pathogenicity of colletotrichum gloeosporioides of the cunninghamia lanceolata.

The invention discloses a medicine for preventing and controlling magnaporthe oryzae. According to the invention, research results find that hesperetin can interfere with conidia to form appressorium, has a remarkable delaying effect on the formation of magnaporthe oryzae appressorium, has an obvious inhibiting effect on magnaporthe oryzae and inhibits the pathogenicity of magnaporthe oryzae, so that the hesperetin has good application value in the aspects of magnaporthe oryzae prevention and control and drug development.

The invention belongs to the production field of industrial seedling culture for aquaculture and particularly relates to egg bag collecting and hatching devices for industrial seedling culture of Rapana venosa and a use method of the devices. The egg bag collecting device comprises attachment devices and a floating frame, the hatching device comprises floating balls, supports and attachment devices, the floating balls are connected to tops of the supports, and the attachment devices and the supports are connected through tail hooks; during egg bag collection, the attachment devices are fixed on the inner wall of the floating frame for culturing parents of Rapana venosa, the attachment devices on the top layer are kept underwater, and the attachment devices with egg bags attached are replaced with new attachment devices with no egg bags attached and then taken out of the floating frame after egg collection; during egg bag hatching, the attachment devices with egg bags attached are hung on the tail hooks of the supports and suspended in a hatching tank for egg bag hatching by the aid of the supports and the floating balls. By means of the devices, the egg bags of the Rapana venosa can be collected conveniently and efficiently and prevented from being damaged due to manual separation of the egg bags, so that the hatching process of the egg bags is finished smoothly.

The invention relates to a biological control method for rice blast and relates to the technical field of biological control of rice pests and diseases. The preparation method comprises preparing a gradient diluent, preparing a NA solid medium, separating bacilli, preparing an LB liquid culture solution, preparing a crude extract protein liquid and carrying out rice colonization. The isolated bacilli can secrete antibacterial substances to inhibit the growth of pathogenic bacteria and induce the plant defense system to resist invasion of pathogenic bacteria. The tested strain has remarkable effects of inhibiting the germination of the magaporthe grisea conidiophores, the prolongation of germ tubes and the formation of appressorium. The active substances of the strain have a strong inhibitory effect on magaporthe grisea and a magaporthe grisea mycelium growth inhibition rate of 80% or more.

The invention discloses a preparation method of a compound agent for prevention and treatment of astragalus powdery mildew. The compound agent comprises the following components by weight: 40-60 partsof a glycolysis solution, 3-8 parts of a surfactant, 0.1-1 part of rosmarinic acid, 0.1-1 part of chitosan, and 0.02-0.04 part of tetrabutyl titanate. The invention has the beneficial effects that: because of the interaction of chitosan and tetrabutyl titanate in the above-mentioned compound agent, the compound agent can form a very thin film on astragalus leaves after use, thus prolonging the action time of the compound agent; rosmarinic acid can improve the killing power of active ingredients in the glycolysis solution to astragalus powdery mildew pathogen; the surfactant can improve the compatibility of the compound agent and astragalus leaves, all the components in the compound agent have coupling effect, can effectively inhibit the spore germination and appressorium formation of astragalus powdery mildew pathogen, and affect the morphology and structure of hypha cells, thus effectively preventing and treating astragalus powdery mildew.

The invention discloses a preparation method of a microbial pesticide for controlling rice blast, and relates to the technical field of biological control of rice diseases and pest insects; the preparation method includes the steps: preparation of a gradient dilution liquid, preparation of an NA solid culture medium, bacillus separation, preparation of a PDA culture medium, and preparation of a spore liquid. Bacillus obtained from isolation can secrete antibacterial substances to inhibit pathogenic bacteria growth, and induces a plant defense system against pathogenic bacteria invasion; a tested strain has a significant inhibitory effect on germination of magnaporthe grisea conidiospores, elongation of germ tubes and appressorium formation; the strain active substances have a relatively strong role in inhibiting magnaporthe grisea, and the magnaporthe grisea mycelium growth inhibition rate reaches 80% or more.

The invention discloses an application of a hydroxy carboxylic acid compound in preventing and treating plant diseases. The hydroxyl carboxylic acid compound is selected from compounds represented byformulas I, II, III and IV, and isomers, hydrates or salts thereof. The hydroxy carboxylic acid compound has a remarkable effect in the aspect of inhibiting the activity of fungus or oomycete appressorium formation, can be used for preventing plant diseases such as rice blast, anthracnose, gray mold, downy mildew and phytophthora, and is free of obvious phytotoxicity and good in safety. Compared with an existing compound for preventing and treating the plant diseases such as rice blast, anthracnose, gray mold, downy mildew and phytophthora, the hydroxyl carboxylic acid compound has the characteristics of good prevention effect, environmental protection, no toxicity, low residue and safety, and as the compound is known and widely used, the raw materials are easy to obtain, the synthesis process is mature, and environmental toxicology and effects are well-studied. Therefore, the advantages of being convenient, easy to obtain, environment-friendly and safe are achieved.

The invention belongs to the production field of industrial seedling culture for aquaculture and particularly relates to egg bag collecting and hatching devices for industrial seedling culture of Rapana venosa and a use method of the devices. The egg bag collecting device comprises attachment devices and a floating frame, the hatching device comprises floating balls, supports and attachment devices, the floating balls are connected to tops of the supports, and the attachment devices and the supports are connected through tail hooks; during egg bag collection, the attachment devices are fixed on the inner wall of the floating frame for culturing parents of Rapana venosa, the attachment devices on the top layer are kept underwater, and the attachment devices with egg bags attached are replaced with new attachment devices with no egg bags attached and then taken out of the floating frame after egg collection; during egg bag hatching, the attachment devices with egg bags attached are hung on the tail hooks of the supports and suspended in a hatching tank for egg bag hatching by the aid of the supports and the floating balls. By means of the devices, the egg bags of the Rapana venosa can be collected conveniently and efficiently and prevented from being damaged due to manual separation of the egg bags, so that the hatching process of the egg bags is finished smoothly.

The invention discloses a biological prevention and control technology for rice blast, and relates to the technical field of biological prevention and control of rice diseases and pests. The preparation method includes the steps of preparing a gradient diluent, preparing an NA solid culture medium, separating bacilli, preparing a PDA culture medium, preparing a spore solution, preparing extracts of murraya paniculata leaves and planting the rice. The bacilli separated according to the method can secrete antibiotic substances to prohibit growth of pathogenic bacteria and entice a plant defencesystem to resist invasion of pathogenic bacteria, and supplied testing bacterial strains can achieve an obvious prohibition effect on germination of mitogenetic spores, extension of germ tubes and formation of appressorium of the rice blast bacteria. The active substances in the bacterial strains have a great prohibition effect on the rice blast bacteria, and the prohibition rate to the mycelial growth of the rice blast bacteria reaches 80% or above. The extracts of the murraya paniculata leaves have a capability of resisting the pathogenic bacteria, insect antifeedant activity and a certain prohibition effect on the rice blast pathogenic bacteria, wherein the callus extracts have excellent bacteriostatic activity, and the bacteriostatic activity to the rice blast bacteria reaches 90% or above.

The invention discloses a new necessary MgPTH12 gene and utility to make pear spore bacterium attach to mature and lead to disease, which is characterized by the following: the gene and its cDNA and coding product possess the sequence in the SEQ ID No.:1, No.:2 and No.:3; the protein of coded gene possesses homeo structural region, which is not similar to known functional protein; the gene removes abnormal and matured attaching cell, which reduces forming frequency of infecting tin and extending infecting hypha without pathogenic ability for rice; the homologous gene of gene lies in the plant pathogenic bacteria widely, which can be applied to design and sieve new drug to prevent fungus.

The invention discloses a rice blast prevention and cure method, and relates to the technical field of biological prevention and cure of rice diseases and pests. A preparation method of the rice blastprevention and cure method comprises the steps of gradient dilution solution preparation, NA solid medium preparation, bacillus separation, PDA medium preparation, spore solution preparation and riceplanting. According to the rice blast prevention and cure method, bacilli obtained through separation can secret antibacterial material to inhibit growth of pathogenic bacteria and induce plant defense systems to defend against invasion of the pathogenic bacteria; tested bacterial strains have significant inhibition action on germination of conidia of magnaporthe grisea, germ tube extending and appressorium forming; active substances of the bacterial strains have strong inhibition action on the magnaporthe grisea, and the inhibition rate of growth of magnaporthe grisea hyphae reaches 80% or above.

The invention provides a sanguinarine compound and application thereof in resisting rice blast, the sanguinarine compound exists in the rhizome of opium poppy plant sanguinarine, is a natural alkaloidwith antibacterial, anti-inflammatory and antioxidant properties, and also has the effects of inhibiting tumor cell proliferation and promoting apoptosis. The influence of the sanguinarine compound added in vitro on magnaporthe grisea hypha growth, spore germination, appressorium development and pathogenicity is detected, and the result shows that the expression of rice disease resistance relatedgenes can be effectively activated by adding a certain concentration of sanguinarine to an external source so that the pathogenicity of magnaporthe grisea is remarkably inhibited. The invention provides an inhibition effect of sanguinarine on magnaporthe oryzae pathopoiesis, which indicates that the compound can be used for preventing and treating the magnaporthe oryzae.

The invention discloses the inhibitory activity of polycarboxylic acid compounds on phytopathogenic fungi and oomycete appresses and their application in the prevention and treatment of plant diseases; the polycarboxylic acid compounds are selected from compounds of formula I, their isomers, hydrated food or salt. The polycarboxylic acid compound involved in the present invention can significantly inhibit the formation of appresses of fungi and oomycetes, and can be used for the prevention of various plant diseases such as rice blast, anthracnose, gray mold, downy mildew, phytophthora, powdery mildew, etc. , no obvious harm, good safety. Compared with the existing compounds for preventing and controlling plant diseases such as rice blast, anthracnose, gray mold, downy mildew, phytophthora, and powdery mildew, the polycarboxylic acid compound of the present invention has good preventive effect, environmental protection, and nontoxicity. , low residue, safety features. Because these compounds are known and widely used compounds, and the environmental toxicology and impact research is thorough, therefore, they have the advantages of convenience, easy availability, environmental protection and safety.

The invention discloses application of 9-phenanthrenol in prevention and treatment of rice blast. Experiments show that the compound can effectively inhibit mycelial growth and spore germination of magnaporthe oryzae, can interfere conidia to form appressorium, has an obvious inhibition effect on magnaporthe oryzae, and inhibits pathogenicity of the magnaporthe oryzae, so that the compound has a very good application value in the aspects of magnaporthe oryzae prevention and control and drug development.

The invention discloses a fermentation filtrate of Chinese fir endophytic fungi and an extract thereof, a preparation method and application thereof. of the present invention Epicoccum dendrobii The fermentation filtrate of SMEL can significantly inhibit the spore germination of Chinese fir anthracis and inhibit the appressorium development of Chinese fir anthracis; Epicoccum dendrobii The ethyl acetate extract of the SMEL fermentation filtrate can significantly inhibit the growth of mycelium of B. fir, and significantly inhibit the spore germination and appressorium development of B. fir. The inoculation test showed that the ethyl acetate extract significantly inhibited the growth of F. anthracis. Sickness.

The invention discloses a specific promoter of pathogenic filamentous fungi. The nucleotide sequence of the specific promoter at least contains a DNA sequence represented by SEQ ID No.1, or a DNA sequence complementary to the sequence represented by the SEQ ID No.1, or a DNA sequence which is 90 or above familiar with the sequence represented by the SEQ ID No.1 or the complementary sequence of the sequence represented by the SEQ ID No.1 and has the same functions with the sequence represented by the SEQ ID No.1 or the complementary sequence of the sequence represented by the SEQ ID No.1. Experiment researches prove that the promoter can be specifically and effectively expressed in a period of appressorium. The promoter provides an important tool for studying the regulation of the pathogenic filamentous fungi in an appressorium formation and permeation into body wall of a host and reconstructing wild insect pathogenic strain gene engineering and has a very high application value.

The invention provides a method for scale breeding of south-migrated stichopus japonicus. The method comprises the steps: selecting mature individuals, which grow for two years in a coastal area, as parental stichopus japonicus, putting the selected parental stichopus japonicus into a breeding pond, and then, stimulating the parental stichopus japonicus to liberate sperms and ova through heating; removing excess male parental stichopus japonicus after heating is ended, then, washing zygotes in the parental stichopus japonicus breeding pond, and hatching in the pond, so as to obtain juvenile; siphoning the juvenile, which moves agilely in an upper layer of water of the breeding pond, to a juvenile breeding pond for juvenile breeding, so as to obtain juvenile stichopus japonicus; attaching the juvenile stichopus japonicus to an attachment device, and suspending the stringed attachment device into a juvenile stichopus japonicus breeding pond, where impurities are discharged, for juvenile stichopus japonicus breeding. According to the method, the problems of high cultivation cost and high death rate caused due to the fact that a large amount of stichopus japonicus seedlings are purchased from the north can be avoided, so that the method provided by the invention is applied to the scale breeding of the south-migrated stichopus japonicus which is suitable for Fujian and neighboring regions thereof.

The invention discloses the application of a blast fungus gene MoRMD1 in regulating the pathogenicity of blast fungus, belonging to the field of plant genetic engineering. The invention constructs a gene knockout vector and introduces it into the protoplast of Magnaporthe oryzae; knocks out the gene MoRMD1 from Magnaporthe oryzae by using a homologous recombination method to obtain a knockout mutant ΔMoRMD1; the obtained Magnaporthe oryzae knockout The mutants are defective in appressorium formation, and MoRMD1 is important for maintaining cell wall integrity. Pathogenicity tests showed that the deletion of MoRMD1 significantly reduced the virulence of Magnaporthe oryzae, and no obvious lesions could be formed on rice leaves. The present invention demonstrates that MoRMD1 is required for appressorium formation, maintenance of cell wall integrity and pathogenicity of M. oryzae. Our research helps to elucidate the pathogenic molecular mechanism of Magnaporthe grisea and provides target genes for the development of effective fungicides.

The invention discloses inhibitory activity of polycarboxylic acid compounds on plant pathogenic fungi and oomycetes appressorium and an application of the polycarboxylic acid compounds in preventionand treatment of plant diseases. The polycarboxylic acid compound is selected from a compound shown as a formula I, and an isomer, hydrate or salt thereof. The polycarboxylic acid compound disclosed by the invention remarkably inhibits the formation of appressorium of fungi and oomycetes, can be used for preventing various plant diseases such as rice blast, anthracnose, gray mold, downy mildew, phytophthora root rot and powdery mildew, and is free of obvious phytotoxicity and good in safety. Compared with an existing compound for preventing and treating plant diseases such as rice blast, anthracnose, gray mold, downy mildew, phytophthora root rot and powdery mildew, the polycarboxylic acid compound has the advantages of being good in prevention effect, environmentally friendly, free of toxicity, low in residue and safe. The compounds are known and widely used, and environmental toxicology and influence research is thorough, so that the compounds have the advantages of convenience, easiness in obtaining, environmental protection and safety.

The invention discloses a preparation method of a pomegranate peel polyphenol botanical fungicide. The pomegranate peel polyphenol botanical fungicide comprises the following components by weight: 40-60 parts of glycolysis liquid, 3-8 parts of a surfactant, 0.1-1 part of pomegranate peel polyphenol, 0.1-1 part of chitosan and 0.02-0.04 part of n-butyl titanate. The invention ahs the beneficial effects that: through the interaction of chitosan and n-butyl titanate in the above-mentioned compound agent, the compound agent can form a layer of very thin film on plant leaves after use, thus prolonging the action time of the compound agent; pomegranate peel polyphenol can improve the killing power of active ingredients in the glycolysis liquid to pathogens; the surfactant can improve the affinity of the compound agent and plant leaves, and all the components in the compound agent have a coupling effect, can effectively inhibit pathogen spore germination and appressorium formation, and affectthe morphology and structure of hypha cells, thus effectively controlling fungal plant diseases.

The invention discloses a filamentous fungi promoter and a plasmid containing the same. The filamentous fungi promoter or a complementary strand thereof has a base sequence shown in SEQ ID (sequence identity) NO.1. The filamentous fungi promoter is a strong promoter, and can perform high-intensity promotion in a mycelium stage, a spore stage and an appressorium stage of pyricularia grisea. The gene of the promoter belongs to the gene, of which the expression intensity is stabilized within the first 100 regions inpyricularia grisea cells. The gene is a histone gene, and plays an important rolein maintaining the normal function of the cells. By utilizing the filamentous fungi promoter, a system that can express in filamentous fungi rapidly and efficiently can be built, so that the researchon the positioning of protein in pathogenic fungi cells as well as on the influence on pathogenic fungi caused by protein over-expression is facilitated.

The invention discloses a medicine for preventing and controlling rice blast fungus. The research of the present invention finds that tangeretin can interfere with the formation of appresses of conidia, has a significant delay effect on the formation of appresses of rice blast fungus, has obvious inhibitory effect on rice blast, and inhibits the pathogenicity of rice blast fungi. It has good application value in the prevention and control of plague and drug development.

The invention discloses a material for inducing appresses of corn leaf spot fungus and its preparation method and application, and belongs to the technical field of research on the infection mechanism of plant pathogenic fungi. The material is made of the following raw materials by volume: 4-6 parts of Si-PUD resin copolymerized with silicone diol and aliphatic isocyanate, and 4-6 parts of self-curing liquid polytetrafluoroethylene resin. The materials for inducing appresses of corn leaf spot fungus of the present invention have safe raw materials, simple preparation, and convenient use, are suitable for high-throughput systems, are used for inducing appresses of corn leaf spot fungi, have high induction efficiency, and are suitable for the infection mechanism of corn leaf spot fungus. Research.