34 results about "Appressorium" patented technology

The invention belongs to the production field of industrial seedling culture for aquaculture and particularly relates to egg bag collecting and hatching devices for industrial seedling culture of Rapana venosa and a use method of the devices. The egg bag collecting device comprises attachment devices and a floating frame, the hatching device comprises floating balls, supports and attachment devices, the floating balls are connected to tops of the supports, and the attachment devices and the supports are connected through tail hooks; during egg bag collection, the attachment devices are fixed on the inner wall of the floating frame for culturing parents of Rapana venosa, the attachment devices on the top layer are kept underwater, and the attachment devices with egg bags attached are replaced with new attachment devices with no egg bags attached and then taken out of the floating frame after egg collection; during egg bag hatching, the attachment devices with egg bags attached are hung on the tail hooks of the supports and suspended in a hatching tank for egg bag hatching by the aid of the supports and the floating balls. By means of the devices, the egg bags of the Rapana venosa can be collected conveniently and efficiently and prevented from being damaged due to manual separation of the egg bags, so that the hatching process of the egg bags is finished smoothly.

The invention discloses a new necessary MgPTH12 gene and utility to make pear spore bacterium attach to mature and lead to disease, which is characterized by the following: the gene and its cDNA and coding product possess the sequence in the SEQ ID No.:1, No.:2 and No.:3; the protein of coded gene possesses homeo structural region, which is not similar to known functional protein; the gene removes abnormal and matured attaching cell, which reduces forming frequency of infecting tin and extending infecting hypha without pathogenic ability for rice; the homologous gene of gene lies in the plant pathogenic bacteria widely, which can be applied to design and sieve new drug to prevent fungus.

The invention discloses the inhibitory activity of polycarboxylic acid compounds on phytopathogenic fungi and oomycete appresses and their application in the prevention and treatment of plant diseases; the polycarboxylic acid compounds are selected from compounds of formula I, their isomers, hydrated food or salt. The polycarboxylic acid compound involved in the present invention can significantly inhibit the formation of appresses of fungi and oomycetes, and can be used for the prevention of various plant diseases such as rice blast, anthracnose, gray mold, downy mildew, phytophthora, powdery mildew, etc. , no obvious harm, good safety. Compared with the existing compounds for preventing and controlling plant diseases such as rice blast, anthracnose, gray mold, downy mildew, phytophthora, and powdery mildew, the polycarboxylic acid compound of the present invention has good preventive effect, environmental protection, and nontoxicity. , low residue, safety features. Because these compounds are known and widely used compounds, and the environmental toxicology and impact research is thorough, therefore, they have the advantages of convenience, easy availability, environmental protection and safety.

The invention discloses a fermentation filtrate of Chinese fir endophytic fungi and an extract thereof, a preparation method and application thereof. of the present invention Epicoccum dendrobii The fermentation filtrate of SMEL can significantly inhibit the spore germination of Chinese fir anthracis and inhibit the appressorium development of Chinese fir anthracis; Epicoccum dendrobii The ethyl acetate extract of the SMEL fermentation filtrate can significantly inhibit the growth of mycelium of B. fir, and significantly inhibit the spore germination and appressorium development of B. fir. The inoculation test showed that the ethyl acetate extract significantly inhibited the growth of F. anthracis. Sickness.

The invention discloses a specific promoter of pathogenic filamentous fungi. The nucleotide sequence of the specific promoter at least contains a DNA sequence represented by SEQ ID No.1, or a DNA sequence complementary to the sequence represented by the SEQ ID No.1, or a DNA sequence which is 90 or above familiar with the sequence represented by the SEQ ID No.1 or the complementary sequence of the sequence represented by the SEQ ID No.1 and has the same functions with the sequence represented by the SEQ ID No.1 or the complementary sequence of the sequence represented by the SEQ ID No.1. Experiment researches prove that the promoter can be specifically and effectively expressed in a period of appressorium. The promoter provides an important tool for studying the regulation of the pathogenic filamentous fungi in an appressorium formation and permeation into body wall of a host and reconstructing wild insect pathogenic strain gene engineering and has a very high application value.

The invention discloses the application of a blast fungus gene MoRMD1 in regulating the pathogenicity of blast fungus, belonging to the field of plant genetic engineering. The invention constructs a gene knockout vector and introduces it into the protoplast of Magnaporthe oryzae; knocks out the gene MoRMD1 from Magnaporthe oryzae by using a homologous recombination method to obtain a knockout mutant ΔMoRMD1; the obtained Magnaporthe oryzae knockout The mutants are defective in appressorium formation, and MoRMD1 is important for maintaining cell wall integrity. Pathogenicity tests showed that the deletion of MoRMD1 significantly reduced the virulence of Magnaporthe oryzae, and no obvious lesions could be formed on rice leaves. The present invention demonstrates that MoRMD1 is required for appressorium formation, maintenance of cell wall integrity and pathogenicity of M. oryzae. Our research helps to elucidate the pathogenic molecular mechanism of Magnaporthe grisea and provides target genes for the development of effective fungicides.

The invention discloses a medicine for preventing and controlling rice blast fungus. The research of the present invention finds that tangeretin can interfere with the formation of appresses of conidia, has a significant delay effect on the formation of appresses of rice blast fungus, has obvious inhibitory effect on rice blast, and inhibits the pathogenicity of rice blast fungi. It has good application value in the prevention and control of plague and drug development.

The invention discloses a material for inducing appresses of corn leaf spot fungus and its preparation method and application, and belongs to the technical field of research on the infection mechanism of plant pathogenic fungi. The material is made of the following raw materials by volume: 4-6 parts of Si-PUD resin copolymerized with silicone diol and aliphatic isocyanate, and 4-6 parts of self-curing liquid polytetrafluoroethylene resin. The materials for inducing appresses of corn leaf spot fungus of the present invention have safe raw materials, simple preparation, and convenient use, are suitable for high-throughput systems, are used for inducing appresses of corn leaf spot fungi, have high induction efficiency, and are suitable for the infection mechanism of corn leaf spot fungus. Research.