43results about How to "Inhibition of pathogenicity" patented technology

The invention relates to a biological organic fertilizer. The biological organic fertilizer comprises 600 to 900 parts of animal manure, 400 to 600 parts of sugar filtered mud, 50 to 200 parts of oil cakes, 200 parts of peat, 200 parts of edible fungus residues, 200 parts of straw, and 1 to 2 parts of organic fertilizer production inocula all in parts by mass. The biological organic fertilizer provided by the invention is complete in nutrient and capable of effectively improving the utilization rate of the fertilizer nutrients and has a sustainable ability and the dual effects of increasing the crop yield and improving the quality of agricultural products; the quality increasing of the biological organic fertilizer mainly refers to increasing of vitamins C and beta-carotene and reducing of the nitrate content and increasing of a single (strip) part, namely improvements in appearance and inherent quality; the yield of the crops is increased, and the quality is improved; the problem of crop continuous cropping planting is solved; soil physicochemical and biological characters are improved; the specificity of the general bacterial manure is broken, so that biological organic fertilizer has good social and ecological benefits.

The invention discloses a universal microorganism bacterial agent which is characterized by comprising one or more of bacillus, pseudomonas, composite azotobacter, photosynthetic bacteria, yeast, fusarium oxysporum, actinomycetes, potassium bacteria, phosphate solubilizing bacteria, bifidobacterium, lactic acid bacteria, the bacteria are activated, and meanwhile, the viable count of the bacteria is not less than 10<8> / ml, and the infectious microbe rate is not greater than 3%. According to different applications and characteristics of the bacteria, special proportion and activation are adopted for different amounts for composite culture so as to make the agent into different special dosage forms, functions and effects of single aspect are highlighted, non-leguminous crops are enabled to have the nitrogen fixation function, the functions in cultivation, breeding, environment-friendliness, mariculture, biological preservation and the like are outstanding, and the agent has important significance in prompting animal and vegetable growth, improving product quality, improving product mass, eliminating public environment pollution and the like.

The present invention relates to methods of detecting or identifying inhibitors of a Z-DNA binding ligand to Z-DNA, methods of inhibiting the pathogenicity of an infectious agent, antiviral therapies, and compounds that inhibit complex formation between a Z-DNA binding ligand and Z-DNA.

The invention provides an immunogenic molecular entity, a supramolecular assembly, and an antibody that can be used to inhibit Gram-positive bacterial quorum sensing, prevent infection or development of a disease condition associated with a Gram-positive bacterial infection. The invention also provides methods of inhibiting Gram-positive bacterial quorum sensing, and methods of preventing infection or development of a disease condition associated with a Gram-positive bacterial infection.

The invention relates to a method for manufacturing fermented milk, comprising the following steps: (1) bacillus natto is supplied; (2) a protein fluid is supplied, and the protein fluid is selected from the group of brewed liquid of (a) fresh milk, (b) milk powder and water and (c) soybean milk; (3) the bacillus natto is added into the protein fluid, and the content of the bacillus natto that is added into the protein fluid is between 8.33*10 <5> CFU / ml and 2*10 <9> CFU / ml; (4) the protein fluid that contains the bacillus natto is put into the temperature between 37 DEG C to 60 DEG C for 2 to 24 hours; and (5) the bacillus natto fermented milk is obtained. The invention also provides the fermented milk that is prepared by the method. The bacillus natto fermented milk that is made of bacillus natto of no toxin, no pathogenicity, high activity and capacity of acclimation has good probiotic effect, and as the bacillus natto fermented milk has no thick sour taste, without needing food additives, sucrose, fruit sugar, and the like for counteract the sour taste, thus complying with the principle of health preservation and health.

The invention discloses a bovine-derived anti-staphylococcus aureus single-chain antibody and preparation and application thereof, and relates to the field of single-chain antibody genetic engineering. The single-chain antibody at least comprises a bovine antibody light chain variable region (VL), a bovine antibody heavy chain variable region (VH) and an intermediate linker peptide (Linker), and the connection sequence is VL-Linker-VH. The preparation method comprises the following steps of: obtaining a bovine-derived single-chain antibody gene, constructing a bovine-derived single-chain antibody primary library, enriching and elutriating, and constructing a single-chain antibody prokaryotic expression plasmid pGEX-4T-1-scFv to obtain a single-chain antibody prokaryotic expression protein.The composition can be used for treating bovine mastitis caused by staphylococcus aureus. The single-chain antibody disclosed by the invention can be specifically combined with staphylococcus aureusLukE protein to inhibit the membrane lysis effect of LukED on bovine mammary epithelial cells, so that the adhesion and damage of staphylococcus aureus to bovine mammary epithelial cells are weakened,and the single-chain antibody has a certain function of inhibiting the damage of staphylococcus aureus to mammary glands.

The invention relates to application of baicalin in resisting and preventing bacterial infection and provides application of baicalin which does not bring survival pressure to bacteria and has little probability of generating resistance to drugs in resisting and preventing bacterial infection.

The invention discloses an R.solani AG1-IA pathogenic related gene and application thereof. The invention provides the R.solani AG1-IA pathogenic related gene and a silent target gene segment Rstpp ofthe R.solani AG1-IA pathogenic related gene. By taking the segment as a target, the pathogenic related genes can be silenced, so that the pathogenicity of R.solani AG1-IA is remarkably inhibited. ThedsRNA of the silent target gene segment Rstpp can be used for preparing a product for preventing and treating R.solani AG1-IA. On the basis, the invention also provides a recombinant vector constructed by using dsRNA. The recombinant vector can be transformed to a plant, and the obtained transgenic plant has obvious disease resistance to R.solani AG1-IA. Therefore, the R.solani AG1-IA pathogenicrelated gene and the silent target gene segment Rstpp thereof have wide popularization and application prospects in prevention and treatment of R.solani AG1-IA including construction of R.solani AG1-IA resistance transgenic plants.

A process for preparing the recombinant chicken gamma-interferon (rCHIFN-gamma) with high activirus activity includes such steps as cloning ChIFN-gamma gene from eukaryotic plasmid to transfer carrier by dual enzyme servenings to obtain recombinant transfer plasmid pFASTBACI-ChIFN-gamma, taking DH10Bac competent cell, adding IngpFASTBAC1-ChIFN-gamma, transposition, diluting to culture object by SOD culture medium, coating on Luria plate, culturing, choosing white clony, purifying, naming positive plasmid as Bacmid-ChIFN-gamma, extracting its DNA, and transfecting Sf9 cells. It can be used to suppress the pathogenic action of both MDV GA to CEF and AIV(H5NI) virus to cell, and the reproduction of NDV F48E8 on cells.

The invention discloses application of pyrimidine derivative in preparing medicine for inhibiting a bacterial quorum sensing system. According to the application disclosed by the invention, LasR protein is utilized as target protein to perform virtual screening to obtain the pyrimidine derivative with a higher score, and the pyrimidine derivative is utilized to perform biological activity evaluation on pseudomonas aeruginosa. Experiments prove that the pyrimidine derivative shows down-regulation on three kinds of genes of LasR, LasB and LasI related to the LasR in culture. Effects of the pyrimidine derivative with the higher score in screening results and the target protein are shown to have an inhibition effect on expression of downstream related genes. Namely, the pyrimidine derivative can inhibit quorum sensing signal molecules to finally reduce bacterial biofilm formation and effectively inhibit pathogenicity of virulence factors and quorum sensing of bacteria. Computer virtual screening is a compound screening method with high efficiency and low cost, so that feasibility for application of the technology in studying quorum sensing medicine is achieved.

The medicine for curing myocarditis is characterized by that it is an oral liquor or intravenous injection made up by using total saponin extracted from Chinese medicinal materials sweetvetch root and sterile physiological saline or phosphoric acid buffer solution through a certain preparation process. It possesses the obvious effect for resisting myocarditis virus and inhibiting pathopoiesis of Coxsackie B3 virus cell, as compared with virazole it possesses obvious protection action for myocarditis, and has no any toxic side effects.

The invention discloses a penaeus vannamei flesh freshness retaining device and belongs to the technical field of shrimp flesh freshness retaining. The penaeus vannamei flesh freshness retaining device comprises a screening chamber, wherein the screening chamber is connected with a sprinkling chamber by virtue of a conveyor belt, the sprinkling chamber is connected with a freezing chamber by virtue of a transmission belt, the freezing chamber is connected with a storage chamber by virtue of a conveyor belt, a sieve piece is arranged inside the screening chamber, a rotary drum is arranged inside the sprinkling chamber, and a freezing plate is arranged inside the freezing chamber. The penaeus vannamei flesh freshness retaining device disclosed by the invention is provided with multiple independent units, all the units can be smoothly connected with each other, freshness retaining operation is simple and ordered, a penaeus vannamei flesh freshness retaining process has independence and specialty, interaction effect among all the technologies can be effectively avoided, excellent freshness retaining effect is guaranteed, penaeus vannamei flesh freshness retaining time is long, a streamlined freshness retaining process has relatively high freshness retaining operation efficiency and is also applicable to industrial production, large-batch penaeus vannamei flesh freshness retaining can be met, freshness retaining cost is effectively reduced, and economic value of penaeus vannamei flesh is improved.

The invention relates to bacillus tequilensis S12 for preventing and treating rice blast and an application thereof, and specifically discloses a bacillus tequilensis strain S12, and an application ofthe S12 strain in prevention and treatment of the rice blast. The strain is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 18347. Thebacillus tequilensis S12 strain and metabolites thereof provided by the invention have strong antagonistic effect on magnaporthe grisea, can significantly inhibit the pathogenicity of the magnaporthegrisea, thereby effectively preventing and treating the rice blast; thus, the bacillus tequilensis S12 strain and the metabolites thereof can be used for biological prevention and treatment of the rice blast. In addition, the biological agent, namely the bacillus tequilensis S12 provided by the invention is safe to human and livestock, is free of problems of environmental pollution and the like,has simple preparation method and low cost, and is simple to use.

The invention discloses an N-acylhomoserinelactone degrading enzyme and application thereof. The degrading enzyme contains a gene AhlM, and the nucleotide sequence of the AhlM is SEQ NO. 1. The N-acylhomoserinelactone degrading enzyme containing the gene AhlM can degrade AHL generated by pathogenic bacteria Pectobacterium spp, Pec, is good in heat stability and pH stability and can obviously inhibit soft rot of crops caused by the pathogenic bacteria Pec.

The present invention provides an anti-Candida albicans piperazine derivative, a preparation method therefor and application thereof. The structural formula of the piperazine derivative is as represented by formula I. The piperazine derivative has the advantages of that the piperazine derivative is a compound capable of inhibiting Candida albicans adhesion, hyphal morphology conversion and pathogenicity, and at the same time, the compound has low toxicity and does nto influence growth of Candida albicans and cells of humans. Therefore, the piperazine derivative is expected to be developed intoa new anti-Candida albicans infection drug.

The invention belongs to the field of biomedicines and in particular relates to a compound for preventing and controlling candida albicans as well as a preparation method and application thereof. Thecompound provided by the invention is 3-methyl-N-(2'-phenemyl)butyramide; an actinomyces streptomyces olivaceus SCSIO T05deltarsdK2 / deltaxmcP mutant strain is constructed through in-frame deletion; amplified fermentation is carried out through a mutant strain fermentation tank, and bacterium collection and centrifuging are carried out; part of mycelia are subjected to ultrasonic extraction throughacetone and part of supernatant is extracted through butanone; extract is combined according to an HPLC (High Performance Liquid Chromatography) analysis result; the compound is obtained by separating through various column chromatography methods. Compared with the prior art, the compound provided by the invention has the beneficial effects that the technology disclosed by the invention successfully prepares and screens the compound 3-methyl-N-(2'-phenemyl)butyramide which has the effects of inhibiting the adhesion of the candida albicans, the mycelium shape transformation and the pathogenicity; meanwhile, the compound 3-methyl-N-(2'-phenemyl)butyramide has relatively small toxicity, does not influence the growth of the candida albicans and human cells and is hopeful to promote and propelthe development of treatment of novel anti-fungus medicines.

The invention provides a compound sanguinarine and its anti-rice blast function. The compound exists in the rhizome of Papaveraceae Sanguinarum, and is a natural alkaloid with antibacterial, anti-inflammatory and anti-oxidative properties. Inhibit tumor cell proliferation and promote apoptosis. The present invention detects its effects on the mycelial growth, spore germination, appressorium development and pathogenicity of Magnaporthe grisea by adding sanguinarine compounds in vitro, and the results show that adding a certain concentration of sanguinarine from exogenous sources can effectively activate rice disease resistance-related genes expression, thereby significantly inhibiting the virulence of Magnaporthe grisea. The invention proposes the inhibitory effect of sanguinarine on the pathogenicity of rice blast fungus, indicating that the compound can be used for preventing and treating rice blast.

The invention discloses a Rhodopseudomonas palustris Atps2 protein and its preparation method and application, and belongs to the technical field of biological pesticides; the Rhodopseudomonas palustris Atps2 protein is composed of The ATPS2 gene of Rhodopseudomonas palustris was prokaryotically expressed in Escherichia coli and obtained through protein purification; the Rhodopseudomonas palustris LY-6 strain was preserved in the China Center for Type Culture Collection, The deposit number is CCTCC NO: M2014525, and the deposit date is November 5, 2014. Aiming at the problem that the existing biopesticides do not effectively prevent and control the rice blast fungus, the present invention provides a Rhodopseudomonas palustris Atps2 protein and its preparation method and application, which can effectively inhibit the rice blast fungus.

The invention provides a sanguinarine compound and application thereof in resisting rice blast, the sanguinarine compound exists in the rhizome of opium poppy plant sanguinarine, is a natural alkaloidwith antibacterial, anti-inflammatory and antioxidant properties, and also has the effects of inhibiting tumor cell proliferation and promoting apoptosis. The influence of the sanguinarine compound added in vitro on magnaporthe grisea hypha growth, spore germination, appressorium development and pathogenicity is detected, and the result shows that the expression of rice disease resistance relatedgenes can be effectively activated by adding a certain concentration of sanguinarine to an external source so that the pathogenicity of magnaporthe grisea is remarkably inhibited. The invention provides an inhibition effect of sanguinarine on magnaporthe oryzae pathopoiesis, which indicates that the compound can be used for preventing and treating the magnaporthe oryzae.

The invention relates to the use of an adsorbent to reduce or suppress the pathogenicity or virulence of Clostridium bacteria in vitro, ex vivo or in vivo. The invention may be used in any mammal or environment, and is particularly effective to suppress the pathogenicity or virulence of Clostridium difficile.

The invention belongs to the field of biomedicines and in particular relates to a compound for preventing and controlling candida albicans as well as a preparation method and application thereof. Thecompound provided by the invention is 3-methyl-N-(2'-phenemyl)butyramide; an actinomyces streptomyces olivaceus SCSIO T05deltarsdK2 / deltaxmcP mutant strain is constructed through in-frame deletion; amplified fermentation is carried out through a mutant strain fermentation tank, and bacterium collection and centrifuging are carried out; part of mycelia are subjected to ultrasonic extraction throughacetone and part of supernatant is extracted through butanone; extract is combined according to an HPLC (High Performance Liquid Chromatography) analysis result; the compound is obtained by separating through various column chromatography methods. Compared with the prior art, the compound provided by the invention has the beneficial effects that the technology disclosed by the invention successfully prepares and screens the compound 3-methyl-N-(2'-phenemyl)butyramide which has the effects of inhibiting the adhesion of the candida albicans, the mycelium shape transformation and the pathogenicity; meanwhile, the compound 3-methyl-N-(2'-phenemyl)butyramide has relatively small toxicity, does not influence the growth of the candida albicans and human cells and is hopeful to promote and propelthe development of treatment of novel anti-fungus medicines.

The invention discloses a bovine-derived single-chain antibody against Staphylococcus aureus and its preparation and application, and relates to the field of single-chain antibody genetic engineering. The single-chain antibody at least comprises a bovine antibody light chain variable region (VL), a bovine antibody heavy chain variable region (VH) and an intermediate connecting peptide (Linker), and the connection sequence is VL-Linker-VH; its preparation method comprises obtaining bovine antibody Derived single-chain antibody gene, construct primary library of bovine-derived single-chain antibody, enrich and panning, construct single-chain antibody prokaryotic expression plasmid pGEX‑4T‑1‑scFv, obtain single-chain antibody prokaryotic expression protein; can be applied to the treatment of golden yellow Bovine mastitis caused by staphylococcus. The single-chain antibody of the present invention can specifically bind to the LukE protein of Staphylococcus aureus, inhibit LukED from lysing the membrane of bovine mammary epithelial cells, thereby weakening the adhesion and damage of Staphylococcus aureus to bovine mammary epithelial cells, and has a certain inhibitory effect on bovine mammary epithelial cells. Function of staphylococci in mammary gland injury.

The invention discloses a traditional Chinese medicine composition used for preventing and treating mycoplasmal pneumonia of swine. The traditional Chinese medicine composition comprises the following components in parts by weight as main active components: 20-30 parts of herba houttuyniae, 15-25 parts of membranous milkvetch roots, 15-25 parts of radix stemonae, 15-20 parts of platycodon grandiflorum, 15-20 parts of garlic, 10-20 parts of rhizoma anemarrhenae, 10-15 parts of ephedra, 10-15 parts of roots of redroot gromwell, and 5-10 parts of radix glycyrrhizae. The invention also discloses a preparation method and application of the traditional Chinese medicine composition. Tests show that the traditional Chinese medicine composition can inhibit in-vitro growth of mycoplasma hyopneumoniae, can well control the mycoplasma hyopneumoniae for infection and pathogenicity of mice, and is clinically used for preventing and treating infection and pathogenicity of the mycoplasma hyopneumoniae, has a good pharmaceutical effect and no toxic or side effects, and is convenient to take.

The invention aims to disclose the application of allicveratrine in the preparation of medicines for preventing and treating Candida albicans infection. Through the detection of various indicators such as the effect of the compound allicveratrine on the growth of Candida albicans, mycelia inhibition rate, cytotoxicity, and animal models, the results show that allicveratrine has no obvious effect on the growth of Candida albicans, among which, The compound allicilveratrine can still effectively inhibit the mycelium formation of Candida albicans when the concentration is 50 μM, and the mycelium formation rate is lower than 50%. In addition, allicveratrine has a good protective effect on human cell A549 infected by Candida albicans, and the compound allicveratrine itself has no obvious toxic and side effects on cells. In the mouse oral model, garlic veratrine has a good inhibitory effect on the invasion of Candida albicans.

The invention belongs to the technical field of biomedicines and in particular relates to a 2-alkylamine quinoline compound for preventing and treating candida albicans as well as a preparation methodand application of the 2-alkylamine quinoline compound. The compound is prepared from a compound A (1,2,3,4-Tetrahydroquinolines) and a compound B (O-benzoyl hydroxylamines) as raw materials throughsynthesis. Compared with the prior art, the compound has the advantages and effects that results of former research of the inventor show that a diffusible signal factor (DSF) quorum sensing signal anda derivative thereof are capable of relatively intensely interfering conversion of a yeast state to a hypha state of candida albicans, and the compound capable of inhibiting adhesion, hypha state conversion and pathogenicity of the candida albicans is successfully synthesized and screened in the research. Meanwhile, the compound is relatively small in toxicity, growth of the candida albicans andhuman cells is not affected, and the characteristics have the potential of promoting development of novel antifungal drugs.

The invention discloses an R.solani AG1-IA pathogenic related gene and application thereof. The invention provides the R.solani AG1-IA pathogenic related gene and a silent target gene segment Rstpp ofthe R.solani AG1-IA pathogenic related gene. By taking the segment as a target, the pathogenic related genes can be silenced, so that the pathogenicity of R.solani AG1-IA is remarkably inhibited. ThedsRNA of the silent target gene segment Rstpp can be used for preparing a product for preventing and treating R.solani AG1-IA. On the basis, the invention also provides a recombinant vector constructed by using dsRNA. The recombinant vector can be transformed to a plant, and the obtained transgenic plant has obvious disease resistance to R.solani AG1-IA. Therefore, the R.solani AG1-IA pathogenicrelated gene and the silent target gene segment Rstpp thereof have wide popularization and application prospects in prevention and treatment of R.solani AG1-IA including construction of R.solani AG1-IA resistance transgenic plants.

Various methods and devices of treating infectious diseases are described. In one example embodiment, a method of treating an infectious disease in an individual in need thereof is provided, including administering a therapeutic agent to the individual and electroporating a tissue of the individual, wherein the therapeutic agent is inhalable.