42results about How to "Inhibition of pathogenicity" patented technology

The invention discloses a universal microorganism bacterial agent which is characterized by comprising one or more of bacillus, pseudomonas, composite azotobacter, photosynthetic bacteria, yeast, fusarium oxysporum, actinomycetes, potassium bacteria, phosphate solubilizing bacteria, bifidobacterium, lactic acid bacteria, the bacteria are activated, and meanwhile, the viable count of the bacteria is not less than 10<8>/ml, and the infectious microbe rate is not greater than 3%. According to different applications and characteristics of the bacteria, special proportion and activation are adopted for different amounts for composite culture so as to make the agent into different special dosage forms, functions and effects of single aspect are highlighted, non-leguminous crops are enabled to have the nitrogen fixation function, the functions in cultivation, breeding, environment-friendliness, mariculture, biological preservation and the like are outstanding, and the agent has important significance in prompting animal and vegetable growth, improving product quality, improving product mass, eliminating public environment pollution and the like.

The present invention relates to methods of detecting or identifying inhibitors of a Z-DNA binding ligand to Z-DNA, methods of inhibiting the pathogenicity of an infectious agent, antiviral therapies, and compounds that inhibit complex formation between a Z-DNA binding ligand and Z-DNA.

The invention provides an immunogenic molecular entity, a supramolecular assembly, and an antibody that can be used to inhibit Gram-positive bacterial quorum sensing, prevent infection or development of a disease condition associated with a Gram-positive bacterial infection. The invention also provides methods of inhibiting Gram-positive bacterial quorum sensing, and methods of preventing infection or development of a disease condition associated with a Gram-positive bacterial infection.

The invention relates to a method for manufacturing fermented milk, comprising the following steps: (1) bacillus natto is supplied; (2) a protein fluid is supplied, and the protein fluid is selected from the group of brewed liquid of (a) fresh milk, (b) milk powder and water and (c) soybean milk; (3) the bacillus natto is added into the protein fluid, and the content of the bacillus natto that is added into the protein fluid is between 8.33*10 <5> CFU/ml and 2*10 <9> CFU/ml; (4) the protein fluid that contains the bacillus natto is put into the temperature between 37 DEG C to 60 DEG C for 2 to 24 hours; and (5) the bacillus natto fermented milk is obtained. The invention also provides the fermented milk that is prepared by the method. The bacillus natto fermented milk that is made of bacillus natto of no toxin, no pathogenicity, high activity and capacity of acclimation has good probiotic effect, and as the bacillus natto fermented milk has no thick sour taste, without needing food additives, sucrose, fruit sugar, and the like for counteract the sour taste, thus complying with the principle of health preservation and health.

The invention relates to application of baicalin in resisting and preventing bacterial infection and provides application of baicalin which does not bring survival pressure to bacteria and has little probability of generating resistance to drugs in resisting and preventing bacterial infection.

The invention discloses an R.solani AG1-IA pathogenic related gene and application thereof. The invention provides the R.solani AG1-IA pathogenic related gene and a silent target gene segment Rstpp ofthe R.solani AG1-IA pathogenic related gene. By taking the segment as a target, the pathogenic related genes can be silenced, so that the pathogenicity of R.solani AG1-IA is remarkably inhibited. ThedsRNA of the silent target gene segment Rstpp can be used for preparing a product for preventing and treating R.solani AG1-IA. On the basis, the invention also provides a recombinant vector constructed by using dsRNA. The recombinant vector can be transformed to a plant, and the obtained transgenic plant has obvious disease resistance to R.solani AG1-IA. Therefore, the R.solani AG1-IA pathogenicrelated gene and the silent target gene segment Rstpp thereof have wide popularization and application prospects in prevention and treatment of R.solani AG1-IA including construction of R.solani AG1-IA resistance transgenic plants.

A process for preparing the recombinant chicken gamma-interferon (rCHIFN-gamma) with high activirus activity includes such steps as cloning ChIFN-gamma gene from eukaryotic plasmid to transfer carrier by dual enzyme servenings to obtain recombinant transfer plasmid pFASTBACI-ChIFN-gamma, taking DH10Bac competent cell, adding IngpFASTBAC1-ChIFN-gamma, transposition, diluting to culture object by SOD culture medium, coating on Luria plate, culturing, choosing white clony, purifying, naming positive plasmid as Bacmid-ChIFN-gamma, extracting its DNA, and transfecting Sf9 cells. It can be used to suppress the pathogenic action of both MDV GA to CEF and AIV(H5NI) virus to cell, and the reproduction of NDV F48E8 on cells.

The invention discloses application of pyrimidine derivative in preparing medicine for inhibiting a bacterial quorum sensing system. According to the application disclosed by the invention, LasR protein is utilized as target protein to perform virtual screening to obtain the pyrimidine derivative with a higher score, and the pyrimidine derivative is utilized to perform biological activity evaluation on pseudomonas aeruginosa. Experiments prove that the pyrimidine derivative shows down-regulation on three kinds of genes of LasR, LasB and LasI related to the LasR in culture. Effects of the pyrimidine derivative with the higher score in screening results and the target protein are shown to have an inhibition effect on expression of downstream related genes. Namely, the pyrimidine derivative can inhibit quorum sensing signal molecules to finally reduce bacterial biofilm formation and effectively inhibit pathogenicity of virulence factors and quorum sensing of bacteria. Computer virtual screening is a compound screening method with high efficiency and low cost, so that feasibility for application of the technology in studying quorum sensing medicine is achieved.

The invention discloses an EGCG with an inhibitory effect on streptococcus pneumoniae pathogenicity and an application of EGCG with the inhibitory effect on the streptococcus pneumoniae pathogenicity for confirming that EGCG has no bacteriostatic activity for streptococcus pneumoniae, but can inhibit peptidase activity of SrtA to lower the pathogenicity of the streptococcus pneumoniae; the invention further provides a new pharmaceutical use of EGCG. Drug sensitivity tests, SrtA peptidase activity inhibition tests, Hep2 adherence inhibition tests and treatment tests of streptococcus pneumoniae infected mice confirm that EGCG plays a role of lowering the streptococcus pneumoniae pathogenicity.

The invention discloses a penaeus vannamei flesh freshness retaining device and belongs to the technical field of shrimp flesh freshness retaining. The penaeus vannamei flesh freshness retaining device comprises a screening chamber, wherein the screening chamber is connected with a sprinkling chamber by virtue of a conveyor belt, the sprinkling chamber is connected with a freezing chamber by virtue of a transmission belt, the freezing chamber is connected with a storage chamber by virtue of a conveyor belt, a sieve piece is arranged inside the screening chamber, a rotary drum is arranged inside the sprinkling chamber, and a freezing plate is arranged inside the freezing chamber. The penaeus vannamei flesh freshness retaining device disclosed by the invention is provided with multiple independent units, all the units can be smoothly connected with each other, freshness retaining operation is simple and ordered, a penaeus vannamei flesh freshness retaining process has independence and specialty, interaction effect among all the technologies can be effectively avoided, excellent freshness retaining effect is guaranteed, penaeus vannamei flesh freshness retaining time is long, a streamlined freshness retaining process has relatively high freshness retaining operation efficiency and is also applicable to industrial production, large-batch penaeus vannamei flesh freshness retaining can be met, freshness retaining cost is effectively reduced, and economic value of penaeus vannamei flesh is improved.

The invention relates to bacillus tequilensis S12 for preventing and treating rice blast and an application thereof, and specifically discloses a bacillus tequilensis strain S12, and an application ofthe S12 strain in prevention and treatment of the rice blast. The strain is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 18347. Thebacillus tequilensis S12 strain and metabolites thereof provided by the invention have strong antagonistic effect on magnaporthe grisea, can significantly inhibit the pathogenicity of the magnaporthegrisea, thereby effectively preventing and treating the rice blast; thus, the bacillus tequilensis S12 strain and the metabolites thereof can be used for biological prevention and treatment of the rice blast. In addition, the biological agent, namely the bacillus tequilensis S12 provided by the invention is safe to human and livestock, is free of problems of environmental pollution and the like,has simple preparation method and low cost, and is simple to use.

The present invention provides an anti-Candida albicans piperazine derivative, a preparation method therefor and application thereof. The structural formula of the piperazine derivative is as represented by formula I. The piperazine derivative has the advantages of that the piperazine derivative is a compound capable of inhibiting Candida albicans adhesion, hyphal morphology conversion and pathogenicity, and at the same time, the compound has low toxicity and does nto influence growth of Candida albicans and cells of humans. Therefore, the piperazine derivative is expected to be developed intoa new anti-Candida albicans infection drug.

The invention belongs to the field of biomedicines and in particular relates to a compound for preventing and controlling candida albicans as well as a preparation method and application thereof. Thecompound provided by the invention is 3-methyl-N-(2'-phenemyl)butyramide; an actinomyces streptomyces olivaceus SCSIO T05deltarsdK2/deltaxmcP mutant strain is constructed through in-frame deletion; amplified fermentation is carried out through a mutant strain fermentation tank, and bacterium collection and centrifuging are carried out; part of mycelia are subjected to ultrasonic extraction throughacetone and part of supernatant is extracted through butanone; extract is combined according to an HPLC (High Performance Liquid Chromatography) analysis result; the compound is obtained by separating through various column chromatography methods. Compared with the prior art, the compound provided by the invention has the beneficial effects that the technology disclosed by the invention successfully prepares and screens the compound 3-methyl-N-(2'-phenemyl)butyramide which has the effects of inhibiting the adhesion of the candida albicans, the mycelium shape transformation and the pathogenicity; meanwhile, the compound 3-methyl-N-(2'-phenemyl)butyramide has relatively small toxicity, does not influence the growth of the candida albicans and human cells and is hopeful to promote and propelthe development of treatment of novel anti-fungus medicines.