121 results about "Opium Poppy" patented technology

This disclosure relates to the isolation and sequencing of nucleic acid molecules that encode methyltransferase polypeptides from a Papaver somniferum cultivar; and uses in the production of noscapine and identification of poppy cultivars that include the genes that include these nucleic acid molecules.

The present invention relates to production of alkaloids from poppy plants and in particular togenes encoding enzymes in the alkaloid pathway, to proteins encoded by the gens, to plants transformed or transfected with the genes and to methods of altering alkaloid content or blend of poppy plants.

This disclosure relates to the isolation of a nucleic acid molecule[s] that encode a novel cytochrome P450 and an oxidoreductase from a Papaver somniferum [P. somniferum] cultivar, transgenic cells transformed with said nucleic acid molecule and sequence variants thereof; and including methods for the production of intermediates in the production of morphinans.

A morphine component, e.g., a concentrate of poppy straw, is converted into codeine in high yield and high purity and in a highly controlled manner. The conversion process involves the following steps: (a) providing a solution or suspension of a morphine component in an inert solvent or a mixture of solvents; (b) methylating the resultant solution or suspension with a methylating agent in the presence of an alkaline ingredient; and (c) recovering the resultant codeine as the free base or as a salt.

The invention discloses cytochrome P450 enzyme gene for taking part in sanguinarine and chelerythrine synthesis in macleaya cordata and application of the cytochrome P450 enzyme gene. For the first time, five cytochrome P450 enzyme genes taking part in sanguinarine and chelerythrine synthesis are found in macleaya cordata genome and comprise gene Mc9485, gene Mc2661, gene Mc217, gene MC11229 and gene Mc11218. A brewer's yeast system verifies that the steps of reaction are respectively verified by upstream precursor feeding and synthesis of sanguinarine and chelerythrine intermediate is achieved. According to the cytochrome P450 enzyme gene, the conversion efficiencies of the same function genes in the macleaya cordata, the papaver somniferum and the eschscholzia californica are compared by the brewer's yeast heterogeneous expression system, and the conversion efficiency of the macleaya cordata is obviously higher than those of the papaver somniferum and the eschscholzia californica. According to the cytochrome P450 enzyme gene, a molecular mechanism of the sanguinarine synthesis in the macleaya cordata is further revealed. Thus, a theoretical basis and a molecular assistant breeding target are provided for the breeding of the macleaya cordata with high sanguinarine and chelerythrine content. Meanwhile, valuable experience is provided for in-vitro artificial synthesis of the sanguinarine and the chelerythrine.

The invention discloses a method for treating opium poppy extract, which comprises the following steps: a, adding a polar solvent into the opium poppy extract, and reacting to obtain a first treating fluid; b, adding a filter aid into the first treating fluid, and filtering to obtain a first extract liquid; c, separating the first extract liquid through a chromatographic column which is filled with spherical silica gel of a bonded stationary phase and a mixture of alcohol and inorganic acid serving as a mobile phase; and d, eluting to obtain a fraction containing alkaloid. Compared with the prior art, the method has the advantages of separating morphine, codeine, thebaine and other monomer alkaloids, and the alkaloid monomer has high purity and large yield.

Belonging to the field of traditional Chinese medicines, the invention discloses a drug for treatment of migraine. The drug provided by the invention comprises the following effective raw material components: Clerodendrum japonicum leaves, Parthenocissus tricuspidata, wild opium poppies, pomelo leaves, litmus, roots of Dentiferous Dendropanax, Cynanchum amplexicaule, Orixa japonica, crape myrtle roots, Astilbe chinensis, Piper longum, Hydrocotyle vulgaris, ergot, fructus viticis, radix clematidis and Korean Mondshood Roots. The drug provided by the invention has the efficacy of nourishing liver and dispelling wind, activating blood circulation and inducing resuscitation. Compared with other similar drugs, the drug provided by the invention has the characteristics of precise compatibility, mild drug properties and obvious curative effect, can rapidly ease migraine symptoms, dredge qi, blood and venations of lesions, promote vasodilatation, regulate the body mechanism, excite the inner analgesic system, relieve vasospasm, inhibit platelet aggregation and blood vessel substance release, thus curing diseases. The drug for treatment of migraine has the characteristics of good curative effect, quick effect, no toxic or side effect, convenient taking, easy absorption, low cost, treatment of both symptoms and root causes, and hard recurrence after cure.

The present invention is directed to an improved poppy straw, concentrate of poppy straw and opium of Papaver somniferum for the production of thebaine containing little or no oripavine, codeine or morphine. The present invention also provides plants, stands and seeds of Papaver somniferum and methods for the production of thebaine.

The present invention is directed to an improved poppy straw, concentrate of poppy straw and opium of Papaver somniferum for the production of codeine containing little or no oripavine, morphine or thebaine. The present invention also provides plants, stands and seeds of Papaver somniferum and methods for the production of codeine.

The present invention relates to a real-time fluorescent PCR (polymerase chain reaction) detection method of opium poppy component, and the detection method comprises the following steps: 1, opium poppy seed DNA is used as a template for fluorescent quantitative PCR amplification to obtain a PCR amplification product; and 2, the fluorescence signal of the amplification product is detected, wherein the reaction system for the PCR amplification comprises an opium poppy component amplification specific primer pair and an opium poppy component amplification specific probe. The opium poppy component real-time fluorescent PCR specific primer pair and the opium poppy component real-time fluorescent PCR specific probe have good specificity and high sensitivity, a very good method for quickly and accurately detecting whether a food additive or a flavoring ingredient is added illegally with the opium poppy component is provided, and the method has good application prospects.

The invention relates to the traditional Chinese medicine synthesis pharmaceutical technology field, and concretely relates to a traditional Chinese medicine cough suppressing preparation. The traditional Chinese medicine cough suppressing preparation is characterized in that the preparation is prepared from the following traditional Chinese medicines, by weight, 40-80 parts of catnip, 40-80 parts of radix peucedani, 40-80 parts of balloonflower roots, 40-80 parts of radix stemonae, 40-80 parts of tatarian aster roots, 35-70 parts of dried orange peels, 60-120 parts of cordate houttuynia, 35-70 parts of mint, 40-80 parts of opium poppy shells, 35-70 parts of licorice, 0.5-5 parts of stevioside, 0.5-5 parts of honey and 0.1-0.2 part of sodium benzoate. The preparation employs honey and perfume oil as medicines, the medicine properties are mild, the therapeutic effects are definite, and the preparation is suitable for taking of the aged and children. The preparation has no obvious toxic and side effects or adverse reactions. Clinic usage results show that the preparation takes effects on the medicine application day for the vast majority of patients, cough is relieved, and pharynx tickle is remitted.

The present invention is one kind of hard candy containing deactivated opium poppy seed extractive. The addition of deactivated opium poppy seed extractive as one new kind of food resource makes the hard candy possess fine structure, special taste, special fragrance and certain medicinal functions of preventing and treating swelling and sore throat, moistening throat, clearing away lung heat, eliminating phlegm, etc.