125 results about "Opium Poppy" patented technology

This disclosure relates to the isolation and sequencing of nucleic acid molecules that encode methyltransferase polypeptides from a Papaver somniferum cultivar; and uses in the production of noscapine and identification of poppy cultivars that include the genes that include these nucleic acid molecules.

The present invention relates to production of alkaloids from poppy plants and in particular togenes encoding enzymes in the alkaloid pathway, to proteins encoded by the gens, to plants transformed or transfected with the genes and to methods of altering alkaloid content or blend of poppy plants.

This disclosure relates to the isolation of a nucleic acid molecule[s] that encode a novel cytochrome P450 and an oxidoreductase from a Papaver somniferum [P. somniferum] cultivar, transgenic cells transformed with said nucleic acid molecule and sequence variants thereof; and including methods for the production of intermediates in the production of morphinans.

The present invention is directed to an improved poppy straw, concentrate of poppy straw and opium of Papaver somniferum for the production of thebaine containing little or no oripavine, codeine or morphine. The present invention also provides plants, stands and seeds of Papaver somniferum and methods for the production of thebaine.

The invention discloses flavoprotein oxidase genes of macleaya cordata in synthesis of sanguinarine and chelerythrine and application of the flavoprotein oxidase genes. Three flavoprotein oxidase genes joining in synthesis of sanguinarine and chelerythrine are found in a macleaya cordata genome for the first time, including genes Mc20113, Mc6407 and Mc6408. Steps of reaction are respectively verified by using a brewer's yeast system through upstream precursor feeding, and synthesis of sanguinarine and chelerythrine and midbodies can be achieved. The conversion efficiency of same functional genes of macleaya cordata and opium poppy is further compared by using a brewer's yeast heterologous expression system, and the result shows that the conversion rate of the gene of macleaya cordata is remarkably higher than that of opium poppy. The invention further discloses a molecular mechanism of synthesis of sanguinarine in macleaya cordata, and a theoretic basis and molecular assistant breeding targets are provided for breeding of macleaya cordata with high contents of sanguinarine and chelerythrine; and meanwhile, precious experience is provided for in-vitro artificial synthesis of sanguinarine and chelerythrine.

A morphine component, e.g., a concentrate of poppy straw, is converted into codeine in high yield and high purity and in a highly controlled manner. The conversion process involves the following steps: (a) providing a solution or suspension of a morphine component in an inert solvent or a mixture of solvents; (b) methylating the resultant solution or suspension with a methylating agent in the presence of an alkaline ingredient; and (c) recovering the resultant codeine as the free base or as a salt.

This disclosure relates to the isolation and sequencing of nucleic acid molecules that encode methyltransferase polypeptides from a Papaver somniferum cultivar; and uses in the production of noscapine and identification of poppy cultivars that include the genes that comprise said nucleic acid molecules.

The invention discloses cytochrome P450 enzyme gene for taking part in sanguinarine and chelerythrine synthesis in macleaya cordata and application of the cytochrome P450 enzyme gene. For the first time, five cytochrome P450 enzyme genes taking part in sanguinarine and chelerythrine synthesis are found in macleaya cordata genome and comprise gene Mc9485, gene Mc2661, gene Mc217, gene MC11229 and gene Mc11218. A brewer's yeast system verifies that the steps of reaction are respectively verified by upstream precursor feeding and synthesis of sanguinarine and chelerythrine intermediate is achieved. According to the cytochrome P450 enzyme gene, the conversion efficiencies of the same function genes in the macleaya cordata, the papaver somniferum and the eschscholzia californica are compared by the brewer's yeast heterogeneous expression system, and the conversion efficiency of the macleaya cordata is obviously higher than those of the papaver somniferum and the eschscholzia californica. According to the cytochrome P450 enzyme gene, a molecular mechanism of the sanguinarine synthesis in the macleaya cordata is further revealed. Thus, a theoretical basis and a molecular assistant breeding target are provided for the breeding of the macleaya cordata with high sanguinarine and chelerythrine content. Meanwhile, valuable experience is provided for in-vitro artificial synthesis of the sanguinarine and the chelerythrine.

The invention discloses a method for treating opium poppy extract, which comprises the following steps: a, adding a polar solvent into the opium poppy extract, and reacting to obtain a first treating fluid; b, adding a filter aid into the first treating fluid, and filtering to obtain a first extract liquid; c, separating the first extract liquid through a chromatographic column which is filled with spherical silica gel of a bonded stationary phase and a mixture of alcohol and inorganic acid serving as a mobile phase; and d, eluting to obtain a fraction containing alkaloid. Compared with the prior art, the method has the advantages of separating morphine, codeine, thebaine and other monomer alkaloids, and the alkaloid monomer has high purity and large yield.

The invention discloses a preparation method of high-quality loquat syrup. The preparation method is characterized in that each 1000 ml of the loquat syrup is prepared from the following raw materials: 68-72 g of loquat leaves, 48-52 g of pericarpium papaveris, 4-6 g of radix stemonae, 8-10 g of cynanchum glaucescens, 5.5-6.5 g of white mulberry root-barks, 5.5-6.5 g of platycodon grandiflorum, 0.14-0.16 g of menthol, 0.45-0.55 g of citric acid, 590-610 g of sucrose, 2.4-2.6 g of sodium benzoate and 1.3-1.5 ml of banana essence. The preparation method comprises the following steps: decocting the raw materials, concentrating, and blending to prepare 1000 ml of the loquat syrup. The loquat syrup product is a light brown clear liquid, is aromatic in smell, sweet in taste and high and stable in quality, is orally taken for three times each day, has the effects of nourishing yin, astringing the lung, relieving a cough and eliminating phlegm, and is used for treating the cough caused by bronchitis; 15 ml of the loquat syrup product is orally taken each time.

The method of altering the alkaloid composition of a poppy plant comprising the step of applying an effective amount of acylcyclohexanedione compound or agriculturally acceptable salt thereof to said poppy plant or locus thereof. Further, the present invention also provides the method of altering the alkaloid composition of a poppy plant comprising the step of applying an effective amount of methyl jasmonate or agriculturally acceptable salt thereof to said poppy plant or locus thereof.

Belonging to the field of traditional Chinese medicines, the invention discloses a drug for treatment of migraine. The drug provided by the invention comprises the following effective raw material components: Clerodendrum japonicum leaves, Parthenocissus tricuspidata, wild opium poppies, pomelo leaves, litmus, roots of Dentiferous Dendropanax, Cynanchum amplexicaule, Orixa japonica, crape myrtle roots, Astilbe chinensis, Piper longum, Hydrocotyle vulgaris, ergot, fructus viticis, radix clematidis and Korean Mondshood Roots. The drug provided by the invention has the efficacy of nourishing liver and dispelling wind, activating blood circulation and inducing resuscitation. Compared with other similar drugs, the drug provided by the invention has the characteristics of precise compatibility, mild drug properties and obvious curative effect, can rapidly ease migraine symptoms, dredge qi, blood and venations of lesions, promote vasodilatation, regulate the body mechanism, excite the inner analgesic system, relieve vasospasm, inhibit platelet aggregation and blood vessel substance release, thus curing diseases. The drug for treatment of migraine has the characteristics of good curative effect, quick effect, no toxic or side effect, convenient taking, easy absorption, low cost, treatment of both symptoms and root causes, and hard recurrence after cure.

The present invention discloses a health-care food, in particular to a Hippophae rhamnoides reviving goyu oil / capsule, which is characterized in that the oil / capsule comprises components with the parts by weight as follows: Hippophae rhamnoides seed oil (30-100 shares), opium poppy seed oil (20-100 shares), grape seed oil (10-50 shares), flaxseed oil (5-20 shares), and menthence oil (1-5 shares). The opium poppy seed goyu oil provide by the present invention has strong functions to regulate the lungs, and the finished product has the scientific prescription, and the Hippophae rhamnoides reviving goyu oil can be encapsulated to make the capsule, the capsule of the Hippophae rhamnoides reviving goyu oil has the advantages of good taste without toxin and harm, the feasibility to be made into both drugs or health-care materials with good functions, easy carrying and wide applicable population.

The invention belongs to the technical field of agricultural machinery, and relates to a small-size chained opium poppy harvesting machine. The opium poppy harvesting machine comprises a harvesting bin, a harvesting table, a transmission system, a conveying device and a wheel type chassis; the harvesting table comprises a reciprocating type cutter arranged at the front end of a bottom plate, multiple harvesting monomers arranged in parallel and a harvesting table driving shaft; each harvesting monomer comprises two bilaterally symmetrical parts, and the left part and right part of each harvesting monomer comprise dividers, guiders, drawing rollers, vertical supporting plates, clamping chains, disc knifes and horizontal mounting plates of the clamping chains respectively; the conveying device comprises a spiral conveyor; the transmission system drives the spiral conveyor, the harvesting table and the reciprocating type cutter. The small-size chained opium poppy harvesting machine has the advantages of being able to adapt to the growth characteristics of opium poppy, meeting the harvesting requirement of the opium poppy, saving manpower and material resources, and improving the laborproduction efficiency.

Plant material enriched in secondary metabolites is produced in tissue culture under conditions that organogenically produce a proliferation of shoots and leafy material, and harvesting the leafy material and shoots while in a green, actively-growing, non-senescent stage. Of particular interest is plant material that produces alkaloids, especially material from poppy (Papaver sp.) that will yield economically attractive levels of morphinane alkaloids, particularly thebaine.

The invention discloses an SSR reagent kit capable of rapidly identifying opium poppy, and relates to the technical field of molecule identification. The SSR reagent kit comprises a set of molecular markers for detecting opium poppy and close species thereof, a PCR amplification reagent and a capillary electrophoresis detection reagent. The set of molecular markers for detecting opium poppy and close species thereof comprise an M13 public fluorescent primer and three specific primer sets; the PCR amplification reagent comprises 2*Taq PCR Master Mix and ddH2O; the PCR amplification reagent includes HI-DI FORMAMIDE BOTTLE and LIZ500. The reagent kit is used for identifying opium poppy homogeneity, siblings, siblings and other species of plants. Compared with the prior art, the higher speciesspecificity and the higher accuracy are achieved for identifying opium poppy and close species plants thereof.

The fragrant poppy seed chili sauce is prepared with poppy seed as main material, chili, onion powder, poppy seed oil, salt, gourmet powder, sugar, aniseed, pricklyash and scallion. The preparation process includes heating poppy seed oil to 110-130 deg.c, frying aniseed, pricklyash and scallion to golden, raising oil temperature to 180-220 deg.c, frying chili powder and onion powder, cooling, frying poppy seed in poppy seed oil, and mixing all the material. The fragrant poppy seed chili sauce has pure fragrance and hot taste and simple preparation process.

The invention discloses a traditional Chinese medicine with anesthetic effect. The traditional Chinese medicine with anesthetic effect is prepared from the following raw materials by weight: 8-10 parts of Houttuynia cordata, 11-16 parts of mint, 2-3 parts of toad venom, 8-10 parts of as arum herb, 8-10 parts of Radix Aconiti Kusnezoffii, 11-16 parts of Arisaema rhizome, 8-12 parts of radix paeoniae alba, 8-10 parts of root of Angelica sinensis, 8-10 parts of Common club moss herb, 8-10 parts of opium poppy shell, 11-16 parts of unprocessed radix aconite, 8-14 parts of Zanthoxylum nitidum, and 8-14 parts of Suberect Spatholobus Stem. The traditional Chinese medicine provided by the invention adopts herbal agents, has small toxic and side effect, is very suitable for local anaesthesia and surface anesthesia, has no side effect after operation, shortens the recovery phase of patients, and is convenient for use. The traditional Chinese medicine is suitable for local anaesthesia and surface skin anesthesia, and the cost is reduced up to 52% than that of chemical anesthesia. The use result of 100 clinical cases shows that the operative anesthesia effective rate is up to 96.4%, local skin is pain free, and the operation process is free of pain.

The invention relates to a bechic medicine, especially to an intensified loquet distillate and its preparation method. The intensified loquet distillate provided by the invention comprises the following components of: by weight, 50 g of loquat leaf, 45 g of opium poppy shell, 10 g of radix stemonae, 6 g of cynanchum root and rhizome, 4 g of mulberry bark, 4 g of balloonflower root and 0.09 g of menthol. The preparation method comprises the following steps of: adding water into six components of loquat leaf and the like except menthol, decocting for two times with 2.5 hours for each time, merging the decoctions, filtering, condensing to 700 ml, adding 2.6 g of sodium benzoate, stirring until it is dissolved, adding 700 g of cane sugar, keeping heating to boiling, insulating for 30 min, standing, filtering, adding water to 1200 ml, and uniformly mixing to obtain the intensified loquet distillate. The intensified loquet distillate provided by the invention has effects of nourishing yin, astringing lung, relieving cough and eliminating sputum, and has a good cough-relieving function.

The present invention is directed to an improved poppy straw, concentrate of poppy straw and opium of Papaver somniferum for the production of thebaine containing little or no oripavine, codeine or morphine. The present invention also provides plants, stands and seeds of Papaver somniferum and methods for the production of thebaine.

The invention is a cough relieving medicine; it is made up of ingredients with following weight proportion: poppy shell immersed plaster 90-110, aster 26-38, chrysoidine 15-26, stemona root radix stemonae 15-26, magnolia vine fruit 3-7, balloonflower root 26-38, citron or trifoliate orange fruit 3-7, dried tangerine peel 26-38, catnip 10-22, hogfennel root 40-54, dried ginger 3-7, liquorice 85-105, ammonium chloride 65-95, peppermint oil dementholized 0.3-0.7ml. The invention can regulate the lung and relieve phlegm, stops cough. The invention also provides the manufacturing method for the medicine.

The invention discloses a poppy species specificity genetic marker detecting system. A primer pair set for identifying poppy is provided and is composed of a primer pair P12 (sequence 1 and 2), a primer pair P13 (sequence 3 and 4), a primer pair P5 (sequence 5 and 6) and a primer pair P14 (sequence 7 and 8). Four STR loci of poppy are amplified at the same time, and the amplification is more accurate and rapid compared with amplification of a single locus; the system has higher species specificity when identifying poppy and affinis plants, and an effective method is provided for further studying poppy STR loci and inspecting and identifying the poppy species in a case; in an optimized poppy STR composite amplification system, amplification of all loci can be balanced, sensitivity is high, stability is high, and frequently-seen corrosion check materials in a drug-related case can be easily inspected; accuracy is high, parting of four loci of each of poppy samples from different producing areas can be achieved through detection and data statistic analysis.

The present invention is directed to an improved poppy straw, concentrate of poppy straw and opium of Papaver somniferum for the production of codeine containing little or no oripavine, morphine or thebaine. The present invention also provides plants, stands and seeds of Papaver somniferum and methods for the production of codeine.

An extract of poppy seed oil rich in linoleic acid, linolenic acid, VE, Se, Mg, amino acids and used in cigarette, chewing gum, and hair and skin protecting products for delaying sanility, relieving fatigue, losing weight, beautifying skin and repairing the damage of cigarette to human body is prepared through extracting in water, extracting in alcohol, concentrating, and drying.

The invention provides a preparation process of cough relieving loquat soft capsules and in particular relates to the technical field of cough relieving medicine preparation. The preparation process is characterized in that the formulation of every 100,000 granules comprises 34.2Kg of loquat leaves, 25Kg of opium poppy capsule, 7.5Kg of sessile stemona root, 4.5Kg of willowleaf swallowwort rhizome, 3Kg of white mulberry root-bark, 2.85Kg of platycodon root, 0.080Kg of menthol, 43Kg of polyethylene glycol (400), 41Kg of gelatin, 14.35Kg of glycerin, 55.35Kg of ethylparaben and 25Kg of light liquid paraffin. The preparation process has the beneficial effects that the preparation process is particular; the medicines are reasonable in proportioning and are packaged by adopting soft capsules, thus being convenient for patients to control the doses; the medicines have obvious effects.

The present invention relates to a real-time fluorescent PCR (polymerase chain reaction) detection method of opium poppy component, and the detection method comprises the following steps: 1, opium poppy seed DNA is used as a template for fluorescent quantitative PCR amplification to obtain a PCR amplification product; and 2, the fluorescence signal of the amplification product is detected, wherein the reaction system for the PCR amplification comprises an opium poppy component amplification specific primer pair and an opium poppy component amplification specific probe. The opium poppy component real-time fluorescent PCR specific primer pair and the opium poppy component real-time fluorescent PCR specific probe have good specificity and high sensitivity, a very good method for quickly and accurately detecting whether a food additive or a flavoring ingredient is added illegally with the opium poppy component is provided, and the method has good application prospects.

The invention relates to the traditional Chinese medicine synthesis pharmaceutical technology field, and concretely relates to a traditional Chinese medicine cough suppressing preparation. The traditional Chinese medicine cough suppressing preparation is characterized in that the preparation is prepared from the following traditional Chinese medicines, by weight, 40-80 parts of catnip, 40-80 parts of radix peucedani, 40-80 parts of balloonflower roots, 40-80 parts of radix stemonae, 40-80 parts of tatarian aster roots, 35-70 parts of dried orange peels, 60-120 parts of cordate houttuynia, 35-70 parts of mint, 40-80 parts of opium poppy shells, 35-70 parts of licorice, 0.5-5 parts of stevioside, 0.5-5 parts of honey and 0.1-0.2 part of sodium benzoate. The preparation employs honey and perfume oil as medicines, the medicine properties are mild, the therapeutic effects are definite, and the preparation is suitable for taking of the aged and children. The preparation has no obvious toxic and side effects or adverse reactions. Clinic usage results show that the preparation takes effects on the medicine application day for the vast majority of patients, cough is relieved, and pharynx tickle is remitted.

There is disclosed an improved poppy straw of a stably reproducing Papaver somniferum for the extraction of thebaine and / or oripavine, the threshed straw having thebaine and oripavine constituting about 50% by weight or greater of the alkaloid combination consisting of morphine, codeine, thebaine and oripavine.

The present invention is one kind of hard candy containing deactivated opium poppy seed extractive. The addition of deactivated opium poppy seed extractive as one new kind of food resource makes the hard candy possess fine structure, special taste, special fragrance and certain medicinal functions of preventing and treating swelling and sore throat, moistening throat, clearing away lung heat, eliminating phlegm, etc.