1786 results about "Disease resistant" patented technology

The invention provides bacillus and application thereof, the bacillus is preserved in Guangdong Microbial Culture Collection Center on June 6, 2025, and the preservation number is GDMCC No.66483; one application of the bacillus is to prepare a preparation for promoting growth and / or resisting diseases of plants, and the other application is to use the bacillus for inhibiting microorganisms; the bacillus can effectively and enduringly inhibit the growth of phytopathogen, and can be used as a broad-spectrum antibacterial biological antibacterial agent to enhance the resistance of plants and promote the growth of the plants.

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

The invention provides a Kitasatospora xanthophycea JY01 strain and application thereof, the strain is separated from intestinal tracts of spring silkworms, has good disease-resistant and growth-promoting effects, and is specifically expressed in that cellulase, beta-1, 3-glucanase, siderophore and auxin IAA can be secreted; pot experiments show that the biocontrol efficiency of the microbial inoculum to wheat stalk rot is 57.90%, the biocontrol efficiency of the microbial inoculum to corn stalk rot is 46.41%, and the plant height and fresh weight are both significantly increased. The microbial agent is expected to be applied to preparation of biological bacterial fertilizer for effectively preventing and controlling soil-borne fungal diseases.

The invention relates to a biological preparation for improving quality of weever, the biological preparation comprises an injection and an edible agent, the injection comprises chemically modified chemically synthesized single-stranded small RNA, Rev-erbalpha protein and Per1 protein, and the edible agent comprises Clock core peptide 1, Clock core peptide 2 and taurine. Skeletal muscle development, liver lipid metabolism and generation of cell inflammatory factors of the weever are influenced by adding and injecting the feed, so that the quality of the weever is improved, intramuscular fat deposition is promoted, the taste and flavor of fish meat are improved, the energy reserve of the weever can be improved, and the culture cycle is shortened. The improvement of cell inflammatory factors shows that the disease resistance of the weever is improved, the survival rate in the breeding process is further improved, and the use amount of antibiotics is reduced. The method provides an important theoretical basis for improving fish meat quality in China and promoting high-quality, efficient and healthy development of aquaculture, and has a wide market prospect.

The invention discloses a seed priming method for improving early-stage disease resistance of pepper seeds, which belongs to the field of agricultural planting and comprises the following steps: S1, pre-cleaning: soaking the seeds with sodium hypochlorite and then cleaning the seeds with sterile deionized water; s2, pulse phosphorylation activation treatment: adding seeds and an activation solution into a pressure-resistant polypropylene container connected with a vacuum pipeline, sealing, vacuumizing to-0.08 MPa, maintaining for 5-10 minutes, then recovering to normal pressure, transferring to a constant-temperature oscillator, and carrying out phosphorylation activation treatment; s3, immune training treatment: transferring the seeds subjected to phosphorylation activation treatment to an initiation solution containing UV-inactivated phytophthora capsici spore suspension and H2O2, and carrying out intermittent ventilation treatment; s4, stabilizing treatment: transferring the seeds subjected to immune training treatment into an ascorbic acid solution for treatment, filtering, and drying in the shade to obtain the pepper seeds. According to the invention, the problem of immune response interruption caused by insufficient signal intensity and fast protein turnover when a simulated environment is used for excitation in the prior art is solved.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention belongs to the field of prevention and treatment of tilletia controversa Kuhn, and particularly relates to a tilletia controversa Kuhn effect protein g16561 and application thereof. An amino acid sequence of the Tilletia controversa Kuhn effect protein is shown as SEQ ID NO: 2. The effect protein plays an important role in the process of inhibiting plant defense reaction, and has an interaction relationship with a wheat protein in the process of infecting wheat by Tilletia controversa Kuhn. According to the invention, the pathogenesis of Tilletia controversa Kuhn can be deeply researched, and the excavation of wheat disease-resistant genes and the cultivation of wheat disease-resistant varieties can be promoted.

The invention relates to the technical field of teasel breeding, and discloses a teasel germplasm resource multi-character collaborative screening breeding method, which comprises the following steps: multi-omics data acquisition: extracting teasel germplasm leaf DNA (deoxyribonucleic acid), and capturing exon regions of disease resistance and metabolism related genes by adopting a targeted sequencing technology to obtain an SNP (single nucleotide polymorphism) marker matrix; the method comprises the following steps: collecting root extracts, detecting medicinal components through ultra-high performance liquid chromatography-mass spectrometry, and constructing a metabolite abundance matrix; measuring phenotype data of plant height, root length, root weight, root rot morbidity and survival rate under drought stress; key site and metabolite correlation analysis: constructing a Bayesian network containing SNP markers, metabolite and phenotypes by using a graph database, and carrying out cross validation through a leave-one-out method. The dipsacus asper germplasm resource multi-character collaborative screening breeding method aims at solving the problem that a traditional breeding method lacks systematic integration analysis of genomes and metabolic pathways in dipsacus asper multi-character collaborative improvement.

The invention belongs to the field of biological organic fertilizers, and discloses a special organic selenium-rich fertilizer for grapes as well as a preparation method and application thereof. The fertilizer is prepared from the following raw materials in percentage by mass: 0.05-0.2% of a selenium complex, 2-15% of a stabilizer, 15-25% of a microbial fermentation product, 0.5-2.0% of a natural plant extract and the balance of auxiliary materials, totaling 100%. The microbial fermentation product is microbial fermentation liquid obtained by fermenting bacillus tequilensis and cyanobacteria; the auxiliary material is obtained by mixing sodium alginate and maltodextrin; the natural plant extract is obtained by mixing azadirachtin, sophocarpidine, tea tree essential oil and cnidium lactone. Through the synergistic effect of the microbial fermentation product, the natural plant extract and the auxiliary materials, the industrial problem that in the prior art, a selenium fertilizer can only improve the selenium content of grapes, but cannot effectively improve the disease resistance and insect pest resistance of the grapes and slow down selenium element loss is solved, and the double improvement of the grape quality and the selenium content is achieved.

The invention relates to the technical field of microorganisms, in particular to a disease-resistant, growth-promoting and yield-increasing strain and a microbial agent and application thereof. The strain is a strain bacillus velezensis NB21 (CCTCC (China Center for Type Culture Collection) NO: M2024979) which is found autonomously; the strain can grow at low temperature, is strong in ecological environment adaptability, can be used for preparing a single microbial agent or a compound microbial agent, and can also be used for forming a composition with other functional organic and inorganic substances. The prepared microbial agent can significantly prevent and treat diseases, promote root development of crops such as rice and corn, enhance photosynthesis, improve system resistance, increase crop yield, enhance nutrient supply and improve soil micro-ecology. The strain is unique, strong in colonization ability and diversified in function, the prepared microbial agent fermentation production technology is mature, the cost is low, the application range is wide, the yield increasing and quality improving effects are outstanding, and the microbial agent has good application prospects.

The invention discloses protease for improving the resistance of potatoes to late blight as well as a coding gene and application thereof, and belongs to the technical field of genetic engineering and plant disease resistance breeding. The amino acid sequence of the protease StRD21 is as shown in SEQ ID NO.2, or the protease StRD21 has a derivative sequence with the same disease-resistant function. The nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. The invention also provides a specific primer pair for cloning the gene, a potato recombinant overexpression vector containing the gene and a transgenic host cell. The StRD21 gene is introduced into potatoes and overexpressed, so that the resistance of the potatoes to late blight can be remarkably enhanced. The invention provides an effective gene resource and a biotechnological means for cultivating a new variety of disease-resistant potatoes.

The invention discloses a preparation process of high-efficiency absorption fish protein peptide and application of the high-efficiency absorption fish protein peptide in fertilizer, and belongs to the technical field of marine organisms. The aquatic product protein is directly used as a fermentation raw material, and the content of amino acid in the fish protein biological peptide is remarkably increased by utilizing the synergistic fermentation effect of microorganisms and assisted by biological enzymolysis, so that the absorptivity of the fish protein peptide is increased; the prepared fish protein peptide is used in the fertilizer to prepare the fish protein peptide functional fertilizer, the nutritional value and the utilization rate of the fertilizer are remarkably improved, and the fish protein peptide functional fertilizer has the remarkable effects of growth promotion, disease resistance, stress resistance and the like. According to the method, a conventional fish protein peptide preparation method of firstly preparing fermentation enzyme liquid and then performing biological enzymolysis is abandoned, the efficient absorption fish protein peptide is prepared by adopting a process of directly fermenting aquatic protein, a fermentation process and an enzymolysis process are integrated, and compared with a conventional process, the method has the advantages of simple production process, low production cost and the like; the method has significant effects and meanings for promoting green preparation of the fish protein peptide and efficient application of the fish protein peptide in fertilizers.

This invention discloses a microbial inoculant for controlling peanut root rot and its preparation method, belonging to the field of microbial materials technology. This invention screened and obtained a *Trichoderma harzianum* strain with highly efficient inhibitory effects against the pathogen causing peanut root rot. Firstly, the strain itself exhibits antagonistic activity against the peanut pathogen. Secondly, it was prepared as a spore suspension and fermented in solid-state with rice husks and wheat bran. During the solid-state fermentation process, the metabolites produced by *Trichoderma harzianum* promote the growth and development of plant roots, increasing plant biomass and yield. This microbial inoculant effectively controls peanut root rot, exhibiting good inhibitory effects against the pathogen, thus improving peanut quality and disease resistance. Furthermore, it improves soil ecological activity to a certain extent, making it worthy of application and promotion.

The invention belongs to the technical field of fertilizers, and particularly relates to a fertilizer for capsicum as well as a preparation method and a fertilization method thereof. In the pepper planting process, the proportion of carbon and nitrogen in the soil is coordinated, the nutrient slow release effect is achieved, the contradiction between vegetative growth and reproductive growth of pepper plants is relieved, and the fertilizer utilization rate is increased. In addition, a microbial agent is applied to the roots after transplanting, and the soil matrix and the disease resistance are enhanced by utilizing the disease resistance and the soil microenvironment improving capability of the microbial agent, so that the pepper yield is increased. The technology is simple and easy to implement, convenient to operate and quick in effect, common farmers or farmers can easily implement the technology, soil fertility and disease resistance can be continuously improved, and the problem of nitrogen and phosphorus loss of the peppers is effectively solved.

The application discloses an NLR plug-in gene for improving plant disease resistance and application thereof, and belongs to the field of plant molecular biology and biotechnology.The NLR plug-in gene StEM1 has a whole gene sequence as shown in SEQ ID NO.1 and a CDS sequence as shown in SEQ ID NO.2, and the amino acid sequence of the encoded protein is as shown in SEQ ID NO.3.The gene plays a key role in improving plant disease resistance, especially resistance to field late blight variant bacteria.In the NLR background, overexpression of the gene significantly promotes the disease resistance of tobacco and potato to pathogenic pythium, and the gene is a gene for enhancing plant disease resistance by improving ETI immunity.Overexpression of the gene in the NLR background is obtained by a genetic transformation method, and a strain line is obtained to resist field variant strains, so as to cope with field diseases of crops.The application provides an excellent gene resource for green prevention and control of late blight and disease-resistant genetic engineering breeding.

The present invention belongs to the technical field of biological prevention and treatment for viral diseases. Disclosed in the present invention are a tomato disease-resistant gene mutant, and the use thereof in the prevention and treatment of ToBRFV. It is found in the present invention that mutating the nucleotide at position 1927 of the coding region sequence of tomato Tm-22 gene from G to A, or mutating the amino acid at position 643 of the LRR domain of a protein that is encoded by tomato Tm-22 gene from glycine to arginine can remarkably reduce the accumulation level of ToBRFV capsid protein (CP). The gene (named Tm-22-Mut5) can serve as a novel ToBRFV resistant gene, and also retains the resistance to TMV, ToMV and ToMMV. The Tm-22-Mut5 and a pre-screened Tm-22-Mut3-1 mutant (tyrosine at position 767 of the LRR domain thereof is mutated to phenylalanine) undergo combinatorial mutagenesis to obtain a mutant Tm-22-Mut6. Analysis shows that the Tm-22-Mut6 can remarkably reduce the accumulation level of the ToBRFV capsid protein. Compared with the pFGCTm-22-Mut3-1 mutant obtained by screening in the previous research and the newly obtained mutant Tm-22-Mut5, the Tm-22-Mut6 has further improved resistance to ToBRFV.

The invention belongs to the field of corn disease prevention, relates to a novel small peptide, provides a small peptide NCRP246 and application thereof in improvement of corn southern leaf blight resistance, and provides feasibility for plant disease resistance by using non-classical peptides. A corn southern leaf blight resistance identification experiment proves that NCRP246 can significantly improve the resistance of corn to southern leaf blight, and further researches that the NCRP246 reduces oxidative damage of pathogenic bacteria infection of the southern leaf blight to the corn by reducing H2O2 accumulation so as to mediate the resistance of the corn to the southern leaf blight; the analysis from the molecular perspective proves that the NCRP246 can induce the expression of defense related genes.

The invention discloses a saline-alkali soil improvement and disease resistance double-effect controlled release fertilizer and a preparation method thereof. The fertilizer comprises 18-22% of biochar, 12-18% of furfural residues, 8-12% of cow dung compost and 6-10% of potassium humate. 5-7% of aluminum sulfate, 4-6% of sulfur and 3-5% of zeolite; the microbial inoculum comprises the following components in percentage by weight: 1.5 to 2.5 percent of saline-alkaline bacillus, 0.8 to 1.2 percent of bacillus subtilis and 0.8 to 1.2 percent 8-12% of a sodium polyacrylate coating, 4-6% of a sulfur coating and 1-3% of polyglutamic acid; 0.8 to 1.2 percent of humic acid chelated iron, 0.8 to 1.2 percent of algal polysaccharide and 0.8 to 1.2 percent of urease inhibitor; 0.8 to 1.2 percent of zinc sulfate, 0.5 to 1.0 percent of manganese sulfate and 0.5 to 1.0 percent of borax. The fertilizer can reduce the pH and conductivity of soil, increase the organic matter content of the soil and effectively improve the physicochemical properties of the soil, meanwhile, by adding the microbial agent, the disease resistance of crops can be effectively improved, the morbidity and disease index of the crops can be reduced, and nutrient release of the fertilizer has a sustained and controlled release characteristic; the initial release rate and the cumulative release rate of nitrogen are both lower than those of common chemical fertilizers, and the cumulative release rate of nitrogen is moderate within 60 days, so that the nutrient requirements of crops in different growth stages can be met.

The invention belongs to the technical field of biological agriculture, and particularly relates to application of a receptor-like kinase gene MdSRF3 and an encoding protein thereof in regulation and control of apple disease resistance. The receptor-like kinase gene MdSRF3 for negative regulation and control of rot resistance is identified from apples, the CDS sequence of the receptor-like kinase gene MdSRF3 is as shown in SEQ ID NO: 1, and the amino acid sequence of coded protein is as shown in SEQ ID NO: 2. Researches show that over-expression of the gene significantly weakens apple disease resistance, and down-regulates expression of defense-related genes; the silence of the gene enhances the disease resistance of the apple. Different from the positive regulation and control functions of homologous proteins in arabidopsis thaliana, potatoes and rice, the negative regulation and control characteristics of the receptor-like kinase gene MdSRF3 provide a new perspective for research of a plant disease-resistant mechanism, provide a new gene resource for apple disease-resistant breeding, and have significant application values and industrialization prospects.

The invention discloses a special bacterial fertilizer for sorghum and application of the special bacterial fertilizer in prevention and control of sorghum diseases, and belongs to the technical field of microbial fertilizers and agriculture. The special bacterial fertilizer for sorghum comprises the following components in parts by weight: 8-10 parts of a composite microbial agent, 40-50 parts of sorghum residues, 5-10 parts of bentonite, 1-2 parts of potassium humate, 1-2 parts of composite trace elements, 10-20 parts of sawdust and 10-20 parts of rice bran, wherein the complex microbial inoculant consists of a bacillus subtilis microbial inoculant, a rhizobium microbial inoculant, a bacillus velezensis microbial inoculant and a trichoderma harzianum microbial inoculant. The special bacterial fertilizer for sorghum provided by the invention contains rich microorganisms and nutrient substances, and can effectively prevent and control sorghum diseases and enhance the disease resistance of sorghum. The components such as microorganisms and organic acids in the special bacterial fertilizer for sorghum can promote the formation of soil organic matters, improve the soil structure, improve the soil quality and effectively improve the growth environment of sorghum.

The invention discloses a pCAMBIA1300-mini RNA3-BC-NLPPya vector as well as a construction method and application of the pCAMBIA1300-mini RNA3-BC-NLPPya vector in high cucumber mosaic virus resistance, the vector is characterized in that mini RNA3-BC-NLPPya is constructed to a pCAMBIA1300 vector skeleton, and the gene sequences of mini RNA3-BC and NLPPya are as shown in SEQ ID NO: 2 and SEQ ID NO: 5. The invention finds that the miniRNA3-BC-NLPPya can efficiently inhibit the infection of the CMV and effectively weaken the harm caused by the CMV, does not need to apply any medicine, is an efficient and green disease-resistant technology, and can provide a new strategy for preventing and controlling the cucumber mosaic virus and similar viruses thereof.

The invention relates to the technical field of biology, in particular to application of a rice OsPBL8 gene in enhancing resistance of rice to magnaporthe oryzae. The rice OsPBL8 gene is a rice endogenous gene participating in the processes of plant growth and development, abiotic stress response, hormone signal channel regulation and control and the like. The invention provides application of the OsPBL8 gene in the field of disease resistance, the OsPBL8 gene is overexpressed through gene editing, and the expression quantity of receptor-like cytoplasm kinase OsPBL8 in plants is increased, so that a plurality of signal channels are regulated and controlled, and the germ resistance of the plants is synergistically improved. By adjusting key hub nodes of plant targeted immune signal transduction, more lasting broad-spectrum disease resistance can be provided, good universality is achieved, disease resistance and growth can be balanced, good practicability is achieved, and improvement of disease resistance of various rice varieties is facilitated.

The invention relates to application of an overexpressed soybean GmMYB306 gene in preparation of a product for improving resistance of crops to soybean phytophthora root rot, and belongs to the technical field of bioengineering. The invention provides application of an overexpressed soybean GmMYB306 gene in preparation of a product for improving the resistance of crops to soybean phytophthora root rot. The nucleotide sequence of the soybean GmMYB306 gene is shown as SEQ ID NO. 1. The soybean GmMYB306 protein disclosed by the invention can interact with a disease-resistant related protein GmNPR1, and the defense capability of soybean plants to phytophthora sojae is improved by regulating and controlling the expression of a crop disease course related gene GmPR1, so that the soybean GmMYB306 protein plays an important role in improving the resistance of the soybean plants to phytophthora sojae root rot.

The invention discloses application of a rice transcription factor OsWRKY39 and a coding gene thereof in rice blast resistance of rice, and belongs to the technical field of gene engineering. According to the invention, an overexpression vector and a gene knockout vector of the rice OsWRKY39 gene are constructed, the vector is transferred into a Kitaake receptor material, an OsWRKY39 overexpression plant and an OsWRKY39 knockout plant are obtained, and the obtained transgenic plants are subjected to disease resistance analysis. Results show that knockout of the OsWRKY39 gene significantly improves the resistance level of rice to rice blast, which indicates that the OsWRKY39 negatively regulates the disease resistance of plants, and the encoding gene OsWRKY39 can be used as a target gene for improving the disease resistance of plants, has a good application prospect in cultivation of disease-resistant rice varieties, and lays an important foundation for rice resistance breeding.

The invention provides application of a TaNAC42 gene in regulation and control of wheat stem rust resistance, and belongs to the technical field of gene functions. It is found for the first time that the TaNAC42 gene of wheat is related to the wheat stem rust resistance, the TaNAC42 gene is induced to be silenced through viruses, the wheat stem rust resistance can be enhanced, wheat plants are easier to resist diseases, the TaNAC42 gene is over-expressed through the agrobacterium tumefaciens-mediated transient over-expression technology, the wheat stem rust resistance can be weakened, the wheat plants are more susceptible to diseases, and the wheat plants are more susceptible to diseases. Namely, the TaNAC42 gene can negatively participate in regulating and controlling the resistance of the wheat to the Puccinia graminicola, a new target gene is provided for improved breeding of wheat Puccinia graminicola resistance molecules, and the TaNAC42 gene has important utilization value in wheat production and breeding.

The invention provides a trichoderma Guizhou B10 strain and application thereof, and relates to the technical field of agricultural microbial control. The trichoderma Guizhou B10 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 3.25634. The invention overcomes the defects in the prior art, and the strain can inhibit pathogenic fungi of malania oleifera leaf spot disease, such as pestalotiopsis, pestalotiopsis, clostridium neocarpum, botryosphaeria and physophaga fungi, can promote the growth of malania oleifera plants, and has both disease-resistant and growth-promoting functions on malania oleifera seedlings, such as malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, and malania oleifera leaf spot disease resistance.

The invention discloses plant probiotics with water retention, growth promotion and disease resistance effects and application thereof. The invention provides a complex microbial inoculant, which is prepared from the following active ingredients: 1) bacillus tequilensis Bac365 (GDMCC No: 65241), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) Enterococcus querebrasis Ent274 (GDMCC No: 65242) or Pseudomonas plecoglossa Pse350 (GDMCC No: 65243), and the active ingredients of the complex microbial inoculant are as follows: 1) bacillus tequilensis Bac365 (GDMCC No: 65243), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) the active ingredients of the complex microbial inoculant. The strain provided by the invention has good nitrogen fixation, phosphorus dissolution, IAA production, HA production and disease resistance, can effectively increase the utilization efficiency of water in drought soil, significantly promote plant growth and increase crop yield, and has good application prospects in the aspects of water retention and sand stabilization, agricultural production and the like.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker-based rice disease resistance character whole genome association analysis method, which relates to the technical field of biology, and is characterized by comprising the following steps: selecting a representative genetic recombination population, carrying out high-density SNP genetic typing on the population, and combining recombination event positioning and linkage analysis to obtain the disease resistance character whole genome association analysis of rice. Obtaining genetic parameters reflecting recombination frequencies of different genome segments; on the basis of the obtained original genetic data, parent and offspring genotype information is further integrated, fine positioning and statistics are carried out on recombination breaking points, and a high-resolution genetic map is constructed. By constructing a high-resolution genetic map and combining feature engineering and machine learning, accurate identification and dynamic SNP encryption of a high-recombination region are realized, the marker coverage and associated signal capture capability of the region is enhanced, the functional site leak detection risk is reduced, and the detection efficiency is improved. The positioning accuracy and the molecular breeding application effect of the rice disease resistance character related candidate gene are improved.

The invention relates to the technical field of plant genetic breeding, in particular to a rice sheath blight resistance related gene and an encoding protein and application thereof. The invention provides a rice sheath blight resistance related gene as well as an encoding protein and application thereof, the gene is OsSBR4 or a related gene thereof, the resistance of rice to sheath blight can be remarkably enhanced by knocking out the gene, and the resistance of rice to sheath blight can be reduced by overexpressing the gene. After agronomic traits of a transgenic line of the gene are investigated, it is found that the plant height, the ear length, the ear number, the thousand seed weight and the like are not affected, and it is indicated that the gene has important application value in sheath blight resistance molecular breeding. According to the invention, a novel rice sheath blight resistance related gene is screened and excavated, and the identification of the sheath blight resistance gene can provide a theory and a basis for breeding a sheath blight resistance rice variety.

The invention relates to a microcapsule slow-release pesticide fertilizer and a preparation method and application thereof.The microcapsule slow-release pesticide fertilizer comprises a capsule core and a capsule wall, materials of the capsule core comprise water-soluble pesticide and fertilizer, materials of the capsule wall comprise silicon dioxide and calcium carbonate, the mass ratio of the silicon dioxide to the calcium carbonate is 0.7: 1-1.6: 1, the median particle size of the microcapsule slow-release pesticide fertilizer is 0.5-5 micrometers, and the median particle size of the microcapsule slow-release pesticide fertilizer is 0.5-2 micrometers. And the surface potential is-30 mV to-52 mV. The microcapsule slow-release pesticide fertilizer not only has excellent slow-release performance, environmental adaptability and environmental friendliness, but also is high in stability, good in dispersity and long in lasting period, so that the utilization rate of the pesticide fertilizer and the disease resistance of crops can be remarkably improved, meanwhile, an acid-base catalyst is not needed in the preparation process, the process is simple, and the production cost is low.