2894 results about "Disease resistant" patented technology

The invention relates to the technical field of microorganisms, in particular to application of a cladosporium spp. Strain and a synthetic flora thereof in plant growth promotion and bacterial wilt prevention and treatment, the cladosporium spp. HX2 is a new strain, has a good symbiotic relationship with tomatoes, has a good prevention effect on tomato bacterial wilt, and has a good application prospect in plant growth promotion and bacterial wilt prevention and treatment. After the bacterial strain is mixed with congeneric strains LJ1, MS2 and LC3 to prepare a cladosporium bacterial agent, the growth-promoting and disease-resisting capabilities of the bacterial agent are enhanced, and experiments show that the synthetic flora has a good growth-promoting function on tomatoes and fresh ginger, the yield of the fresh ginger is remarkably increased, and the growth vigor of tomato plants is improved; meanwhile, the synthetic flora also has a good prevention and treatment effect on bacterial wilt of tomatoes, fresh ginger and tobaccos, and can improve the bacterial wilt resistance of plants, so that the strain HX2 and the synthetic flora prepared from the strain HX2 can be used as an anti-microbial agent or a raw material for preparing a bio-organic fertilizer in the later period.

The present invention relates to the technical field of pesticides, and discloses a biological agent-based rice sheath blight disease efficient control agent, which comprises an active flora, a carrier, an auxiliary agent and a disease resistance enhancing factor, the active flora is used for combining humidity, pH and temperature response type materials so as to make the bacterial agent dynamically released, and the disease resistance enhancing factor is used for controlling the disease resistance of the rice sheath blight disease. The carrier is used for enabling the active ingredients to be slowly released in a field environment and keep stability, the auxiliary additive is used for improving the activity, adhesiveness and soil adaptability of the fungicide, and the disease-resistant enhancing factor is used for expanding and improving the adaptability of the control agent. The ratio of the active flora to the carrier to the auxiliary additive to the disease-resistant enhancing factor is 10: 50: 13: 27. Through the synergistic effect of various functional strains and the combination of the dynamic release carrier, the occurrence and propagation of the rice sheath blight disease are effectively inhibited, the prevention and treatment rate can reach 75-85%, and the prevention and treatment effect is obviously higher than that of a traditional chemical pesticide or a single fungicide.

The invention provides bacillus and application thereof, the bacillus is preserved in Guangdong Microbial Culture Collection Center on June 6, 2025, and the preservation number is GDMCC No.66483; one application of the bacillus is to prepare a preparation for promoting growth and / or resisting diseases of plants, and the other application is to use the bacillus for inhibiting microorganisms; the bacillus can effectively and enduringly inhibit the growth of phytopathogen, and can be used as a broad-spectrum antibacterial biological antibacterial agent to enhance the resistance of plants and promote the growth of the plants.

The invention discloses a microbial agent for preventing and treating soil-borne diseases of food crops and a preparation method of the microbial agent, and belongs to the technical field of microorganisms. The microbial inoculum comprises three functional strains, namely pseudomonas guayensis, paenibacillus polymyxa and trichoderma koningiopsis, the three strains are mixed in equal proportion and cooperate with one another, so that the microbial inoculum has a remarkable disease-resistant effect, has an antagonistic effect on various diseases of wheat and corn, can be used for preventing and treating wheat root rot, take-all and banded sclerotial blight as well as corn stem rot and banded sclerotial blight, and has a wide application prospect. Particularly, the inhibition effect on the take-all disease of wheat is optimal. Meanwhile, the coating agent and the auxiliaries are used, so that the acting durability and stability of the microbial inoculum are improved. The microbial agent is matched with a conventional base fertilizer for use, use of chemical bactericides is reduced, growth of common pathogenic bacteria of food crops is effectively inhibited, and the yield of the food crops is increased.

The invention discloses a macrobrachium rosenbergii iridovirus disease resistance-related SNP (Single Nucleotide Polymorphism) site, a site combination and application thereof, and belongs to the field of aquatic organism breeding. According to the invention, SNP loci related to resistance of the macrobrachium rosenbergii iridovirus disease are successfully screened through whole genome association analysis, and the SNP loci are respectively located at a 9574545th base of a chromosome 10 of the macrobrachium rosenbergii and a 26491028th base of a chromosome 14 of the macrobrachium rosenbergii. Aiming at the two SNP sites, an SNP site combination related to the resistance of the macrobrachium rosenbergii iridovirus disease is further developed, and macrobrachium rosenbergii iridovirus disease resistant individuals can be screened by detecting the SNP site combination. Therefore, the SNP molecular marker provided by the invention can be used for macrobrachium rosenbergii molecular marker assisted breeding, accelerates disease-resistant macrobrachium rosenbergii variety breeding, and provides a reference basis for disease-resistant breeding work of macrobrachium rosenbergii.

The invention discloses application of a wheat WRKY transcription factor gene TaWRKY1, and belongs to the technical field of gene engineering. A nucleotide sequence of an open reading frame of the gene TaWRKY1 is shown as SEQ ID NO: 1, and the gene TaWRKY1 is infected by stripe rust to induce expression in interaction of wheat and stripe rust, and plays a negative regulation role in wheat stripe rust resistance. A TaWRKY1 gene editing mutant plant is created by using an agrobacterium tumefaciens-mediated genetic transformation method, the sporulation quantity on wheat leaves of the gene editing mutant is obviously reduced, and the allergic necrosis is increased, which indicates that the edited gene TaWRKY1 can improve the resistance of wheat to stripe rust. The invention provides a disease-resistant germplasm resource for the cultivation of the stripe rust-resistant wheat variety.

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

The invention discloses a gene OsSK8 for regulating and controlling disease resistance of rice as well as an encoding protein and application of the gene OsSK8, the gene is the gene OsSK8, and the sequence of the gene OsSK8 is shown as SEQ ID NO. 1 or SEQ ID NO. 2. A novel rice sheath blight and bacterial blight resistance regulation gene OsSK8 is identified, the OsSK8 gene is subjected to overexpression and knockout, and after resistance identification, it is found that the overexpression of the OsSK8 gene can enhance the resistance of rice to sheath blight and bacterial blight, and the knockout of the gene can reduce the resistance of rice to sheath blight and bacterial blight. The OsSK8 gene positively regulates the resistance level of rice to sheath blight and bacterial blight, so that a new thought and direction are provided for breeding rice varieties with higher disease resistance, and meanwhile, a solid foundation is laid for further exploration in the field of rice disease-resistant breeding.

The invention discloses application of astaxanthin as a feed additive in improving the disease resistance of macrobrachium rosenbergii, and belongs to the field of feed additives. According to the method, astaxanthin is supplemented in the feed, the survival rate of the juvenile macrobrachium rosenbergii after being infected with DIV1 is increased, meanwhile, the abundance of pathogenic bacteria in intestinal tracts after the macrobrachium rosenbergii is infected with DIV1 is reduced, then the purpose of preventing disease outbreak of the macrobrachium rosenbergii caused by DIV1 can be achieved, and the method is a sustainable and effective method for preventing and treating the macrobrachium rosenbergii DIV1 disease.

The invention discloses a wheat E3 ubiquitin ligase gene RFEL1 and application of the wheat E3 ubiquitin ligase gene RFEL1 in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The invention discloses a nucleotide sequence of a wheat E3 ubiquitin ligase gene RFEL1 as shown in SEQ ID NO. 3. The invention further provides application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof. The application comprises any one of the following applications: A1, improving the resistance of wheat to stripe rust; a2, the resistance of wheat to powdery mildew is improved; and A3, the resistance of wheat to leaf rust is improved. The E3 ubiquitin ligase RFEL1 gene provided by the invention not only has broad-spectrum resistance to different stripe rust, but also has resistance to wheat leaf rust and powdery mildew. The E3 ubiquitin ligase RFEL1 gene disclosed by the invention is applied to broad-spectrum resistance breeding of wheat and has important application value.

The invention relates to the technical field of laying hen breeding, and discloses an automatic acquisition and analysis system for laying hen breeding data. The information acquisition unit is used for collecting physiological parameters, behavior data, environmental parameters and health indexes of laying hens in real time; the disease monitoring unit is used for analyzing the collected data and identifying physiological abnormality and behavior change before the disease occurs; the antibody level monitoring unit is used for evaluating the immune response capability of the laying hens to specific pathogens; the environment adaptability evaluation unit is used for evaluating the adaptability of the laying hens to the extreme environment according to the environment parameter change and the physiological response of the laying hens; the breeding decision support unit is used for integrating all data and analyzing the production performance, disease resistance and environmental adaptability of laying hens; an optimal feeding scheme is recommended, medicine use is reduced, and resource allocation is optimized.

The invention belongs to the technical field of aquatic biotechnology and molecular marker-assisted breeding, and particularly relates to a cynoglossus semilaevis vibrio harveyi-resistant KASP molecular marker combination and application thereof in cynoglossus semilaevis disease-resistant breeding. The KASP molecular marker combination comprises three SNP (Single Nucleotide Polymorphism) loci which are respectively positioned at 17314099, 17325496 and 17355989 loci of the 14 # chromosome of the cynoglossus semilaevis, when the genotypes of the three SNP loci are respectively CC, AA and GG, the disease resistance of the cynoglossus semilaevis is high, and the invention also provides a primer for detecting the genotype polymorphism of the SNP loci. By utilizing the KASP molecular marker combination, the vibrio harveyi resistance genotype in a cynoglossus semilaevis breeding population can be quickly detected, and disease-resistant individuals are screened, so that the disease-resistant breeding efficiency of the cynoglossus semilaevis is greatly improved, and a new variety of the cynoglossus semilaevis with strong disease resistance can be quickly cultivated.

The invention provides a Kitasatospora xanthophycea JY01 strain and application thereof, the strain is separated from intestinal tracts of spring silkworms, has good disease-resistant and growth-promoting effects, and is specifically expressed in that cellulase, beta-1, 3-glucanase, siderophore and auxin IAA can be secreted; pot experiments show that the biocontrol efficiency of the microbial inoculum to wheat stalk rot is 57.90%, the biocontrol efficiency of the microbial inoculum to corn stalk rot is 46.41%, and the plant height and fresh weight are both significantly increased. The microbial agent is expected to be applied to preparation of biological bacterial fertilizer for effectively preventing and controlling soil-borne fungal diseases.

The invention provides a Ustilaginoidea virens gene UvASE2 for regulating and controlling rice resistance and application of the Ustilaginoidea virens gene UvASE2, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the Ustilaginoidea virens gene UvASE2 is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the Ustilaginoidea virens gene UvASE2 is as shown in SEQ ID NO: 2. By constructing a transgenic rice plant for heterologous expression of UvASE2, it is found that the transgenic rice can significantly improve the resistance to false smut and bacterial blight and significantly induce the outbreak of active oxygen. The heterologous expression UvASE2 gene of Ustilaginoidea virens has the function of positively regulating and controlling the disease resistance of rice, and the UvASE2 gene can be used for improving the disease resistance of rice and has important significance on creation of disease-resistant germplasm of rice.

The invention provides bacillus velezensis NR10, and the preservation number of the bacillus velezensis NR10 is CGMCC (China General Microbiological Culture Collection Center) NO.31771. The bacillus velezensis NR10 is a strawberry endophyte, has good bacteriostatic activity, has good antagonistic action on botrytis cinerea, fusarium oxysporum, colletotrichum gloeosporioides, colletotrichum oxysporum, trichoderma harzianum, trichoderma longibrachiatum and the like, is a potential biological preparation for preventing and treating strawberry diseases and the like, and has broad application prospects. And the bacteriostatic action of the bacillus velezensis NR10 has thermal stability. In addition, the bacillus velezensis also has good capacities of producing siderophores, fixing nitrogen and dissolving potassium, producing protease and cellulase and the like. The bacillus velezensis NR10 provided by the invention has wider-spectrum disease resistance, wide development space and good development and application prospects.

The invention discloses a rice OsTCP11 gene and application of the rice OsTCP11 gene in rice disease resistance, and the nucleotide sequence of the OsTCP11 gene is as shown in SEQ ID NO.1; the method is realized by knocking out a rice OsTCP11 gene; the knockout is carried out by utilizing a CRISPR / Cas9 gene editing technology; the gene OsTCP11 is knocked out through a CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats / associated protein 9) technology, and a knockout mutant ostcp11 is obtained; it is found through rice blast resistance identification that compared with wild type rice, the mutant ostcp11 is more resistant to diseases and small in disease spot area, the number of pyricularia grisea carried by diseased leaves is small, and it is indicated that OsTCP11 negatively regulates the resistance of rice to rice blast. The bacterial leaf blight resistance identification finds that compared with wild rice, the mutant ostcp11 is more resistant to diseases and shorter in disease spots, which indicates that the OsTCP11 negatively regulates the resistance of the rice to the bacterial leaf blight. The method is beneficial to cultivation of rice blast and bacterial leaf disease resistant rice varieties, and provides a basis for later screening of high-resistance and broad-spectrum-resistance rice varieties.

The invention discloses a rice OsRH3 gene and application thereof in rice disease resistance, the nucleotide sequence of the rice OsRH3 gene is as shown in SEQ ID NO.1, and the nucleotide sequence of the rice OsRH3 gene is as shown in SEQ ID NO.2. The gene OsRH3 is knocked out through a CRISPR / Cas9 technology, and a knockout mutant osrh3 is obtained; it is found through rice blast resistance identification that compared with wild type rice, the mutant osrh3 is more resistant to diseases and small in disease spot area, the number of pyricularia grisea carried by diseased leaves is small, and it is indicated that the OsRH3 negatively regulates the resistance of rice to rice blast. The bacterial leaf blight resistance identification finds that compared with wild rice, the mutant osrh3 is more resistant to diseases and shorter in disease spots, which indicates that the OsRH3 negatively regulates the resistance of the rice to the bacterial leaf blight. The method is beneficial to cultivation of rice blast and bacterial leaf disease resistant rice varieties, and provides a basis for later screening of high-resistance and broad-spectrum-resistance rice varieties.

The invention provides an ScPsbP gene with disease resistance, a protein and application, belongs to the technical field of biology, and particularly discloses application of the ScPsbP gene of sugarcane in improving the disease resistance. The PsbP gene plays an important role when plants respond to pathogenic bacteria stress, the disease-resistant function of the ScPsbP is preliminarily researched, and a solid foundation is laid for deeply researching the function and the action mechanism of the gene in the process that the plants respond to the pathogenic bacteria stress.

The invention discloses a method for improving citrus yellow vein disease resistance by using a ClPsaN gene. The method comprises the following steps: (1) cloning a VIGS fragment of the citrus ClPsaN gene; (2) constructing two VIGS expression vectors of TRV and CLBV; and (3) transforming the citrus by the VIGS expression vector to obtain a citrus ClPsaN gene silenced VIGS plant. According to the invention, the VIGS vector of the Citrus ClPsaN gene is constructed, and then the Citrus is transformed, so that the obtained Citrus plant can show obvious resistance to the Citrus yellow vein clearing virus, the titer of the Citrus yellow vein clearing virus can be reduced by 22-34%, the accumulation amount of the virus protein is reduced by 35-50%, and the attack degree of the Citrus yellow vein clearing virus is obviously reduced; the TRV silencing of the citrus ClPsaN gene does not influence the phenotype of a citrus plant, and the citrus ClPsaN gene can be used as a candidate gene for citrus yellow vein disease resistance breeding together with a plurality of citrus yellow vein disease-resistant and susceptible genes by utilizing the cooperation of VIGS silencing, RNA interference, gene editing and other technologies.

The invention provides bacillus altitudinis NM62 and application of the bacillus altitudinis NM62 in growth promotion and disease resistance. The preservation number of the bacillus altitudinis NM62 is CGMCC NO.31272. The bacillus altitudinis strain has a good disease prevention effect on tobacco black shank and can promote the growth of tobacco plants. The strain provides a new strain resource for biological prevention and control of tobacco black shank, and provides a theoretical basis for developing a composite biocontrol agent which is environment-friendly, biosafe and lasting in prevention and control effect.

The invention relates to a biological preparation for improving quality of weever, the biological preparation comprises an injection and an edible agent, the injection comprises chemically modified chemically synthesized single-stranded small RNA, Rev-erbalpha protein and Per1 protein, and the edible agent comprises Clock core peptide 1, Clock core peptide 2 and taurine. Skeletal muscle development, liver lipid metabolism and generation of cell inflammatory factors of the weever are influenced by adding and injecting the feed, so that the quality of the weever is improved, intramuscular fat deposition is promoted, the taste and flavor of fish meat are improved, the energy reserve of the weever can be improved, and the culture cycle is shortened. The improvement of cell inflammatory factors shows that the disease resistance of the weever is improved, the survival rate in the breeding process is further improved, and the use amount of antibiotics is reduced. The method provides an important theoretical basis for improving fish meat quality in China and promoting high-quality, efficient and healthy development of aquaculture, and has a wide market prospect.

Rosa chinensis gray mold is a main disease in the cultivation process of rosa chinensis, and seriously threatens the quality and yield of cut-flower rosa chinensis. In order to effectively cope with the disaster stress which influences the yield and quality of the Chinese rose, 192 pairs of SSR primers are used in the research to carry out PCR amplification on DNA of parents' Jinmai '' Beijing red 'and 6 randomly selected offspring. 35 pairs of primers show genetic difference between male and female parents. The 35 pairs of primers are further used for carrying out PCR amplification on filial generation DNA, detection is carried out through PAGE electrophoresis, and an electrophoretic band is converted into a 0, 1 matrix for genetic typing. The method comprises the following steps: selecting an optimal subset through correlation regression analysis, and finally successfully screening out three primers, namely, Geno-BC-1, Geno-BC-2 and Geno-BC-3, which are related to gray mold resistance. The discovery provides an important theoretical basis for molecular marker-assisted Chinese rose disease-resistant breeding, and is expected to promote early identification of disease-resistant plants, so that the breeding period is shortened, and the breeding efficiency is improved.

The invention discloses a seed priming method for improving early-stage disease resistance of pepper seeds, which belongs to the field of agricultural planting and comprises the following steps: S1, pre-cleaning: soaking the seeds with sodium hypochlorite and then cleaning the seeds with sterile deionized water; s2, pulse phosphorylation activation treatment: adding seeds and an activation solution into a pressure-resistant polypropylene container connected with a vacuum pipeline, sealing, vacuumizing to-0.08 MPa, maintaining for 5-10 minutes, then recovering to normal pressure, transferring to a constant-temperature oscillator, and carrying out phosphorylation activation treatment; s3, immune training treatment: transferring the seeds subjected to phosphorylation activation treatment to an initiation solution containing UV-inactivated phytophthora capsici spore suspension and H2O2, and carrying out intermittent ventilation treatment; s4, stabilizing treatment: transferring the seeds subjected to immune training treatment into an ascorbic acid solution for treatment, filtering, and drying in the shade to obtain the pepper seeds. According to the invention, the problem of immune response interruption caused by insufficient signal intensity and fast protein turnover when a simulated environment is used for excitation in the prior art is solved.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention belongs to the technical field of biology, and particularly relates to a difunctional gene cluster biosynthesized by phenol amine and salicylic acid and application of the difunctional gene cluster. The gene cluster comprises five genes, the five genes are S1EPS1, S1PBS3, S1CoAOMT2, S1CoOOMT3 and S1CoAOMT4, the nucleotide sequence of the S1EPS1 gene is as shown in SEQ ID No.1, the nucleotide sequence of the S1PBS3 gene is as shown in SEQ ID No.3, the nucleotide sequence of the S1CoAOMT2 gene is as shown in SEQ ID No.5, the nucleotide sequence of the S1CoOOMT3 gene is as shown in SEQ ID No.7, and the nucleotide sequence of the S1CoAOMT4 gene is as shown in SEQ ID No.9. The invention further discloses a preparation method of the gene cluster. The overexpressed gene cluster component SlEPS1 gene can significantly enhance accumulation of spermidine derivatives and salicylic acid of tomato plants so as to enhance the resistance to Pst DC3000, lays a foundation for genetic improvement in the aspect of tomato disease resistance by using the gene cluster, and also provides technical reference for excavation of related metabolic gene clusters in other crops and in the aspect of resistance breeding.

The invention belongs to the field of prevention and treatment of tilletia controversa Kuhn, and particularly relates to a tilletia controversa Kuhn effect protein g16561 and application thereof. An amino acid sequence of the Tilletia controversa Kuhn effect protein is shown as SEQ ID NO: 2. The effect protein plays an important role in the process of inhibiting plant defense reaction, and has an interaction relationship with a wheat protein in the process of infecting wheat by Tilletia controversa Kuhn. According to the invention, the pathogenesis of Tilletia controversa Kuhn can be deeply researched, and the excavation of wheat disease-resistant genes and the cultivation of wheat disease-resistant varieties can be promoted.

The invention discloses application of a ZmFS1 gene in corn disease prevention and control, and belongs to the technical field of crop disease prevention and control. The nucleotide sequence of cDNA of the ZmFS1 gene is as shown in SEQ ID NO.1. By inhibiting expression of the gene, resistance of corn to various diseases including southern leaf blight, northern leaf blight, southern rust disease, anthracnose, stem rot, seed rot and the like can be remarkably enhanced, a new solution thought is provided for preventing and treating corn diseases from the perspective of molecular biology, and the application prospect is broad. The method can be applied to corn disease-resistant breeding.

The invention relates to the technical field of teasel breeding, and discloses a teasel germplasm resource multi-character collaborative screening breeding method, which comprises the following steps: multi-omics data acquisition: extracting teasel germplasm leaf DNA (deoxyribonucleic acid), and capturing exon regions of disease resistance and metabolism related genes by adopting a targeted sequencing technology to obtain an SNP (single nucleotide polymorphism) marker matrix; the method comprises the following steps: collecting root extracts, detecting medicinal components through ultra-high performance liquid chromatography-mass spectrometry, and constructing a metabolite abundance matrix; measuring phenotype data of plant height, root length, root weight, root rot morbidity and survival rate under drought stress; key site and metabolite correlation analysis: constructing a Bayesian network containing SNP markers, metabolite and phenotypes by using a graph database, and carrying out cross validation through a leave-one-out method. The dipsacus asper germplasm resource multi-character collaborative screening breeding method aims at solving the problem that a traditional breeding method lacks systematic integration analysis of genomes and metabolic pathways in dipsacus asper multi-character collaborative improvement.

The invention discloses Klebsiella oxytoca G1-2-4 and application thereof, and particularly relates to application of the Klebsiella oxytoca G1-2-4 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.30986 in preparation of a biocontrol preparation or feed for preventing and treating aquatic diseases. The invention further discloses application of the Klebsiella oxytoca G1-2-4 in preparation of the biocontrol preparation or feed for preventing and treating the aquatic diseases and application of the Klebsiella oxytoca G1-2-4 in preparation of the biocontrol preparation or feed for preventing and treating the aquatic diseases. The invention relates to an application of klebsiella oxytoca G1-2-4 in inhibiting the growth of pseudomonas plecoglossicida and a biocontrol preparation or feed containing the klebsiella oxytoca G1-2-4 and used for preventing and treating aquatic diseases. According to the Klebsiella oxytoca cultured in an isolated mode, the disease resistance of the Klebsiella oxytoca is verified through in-vitro and in-vivo experiments, the disease resistance mechanism of the Klebsiella oxytoca is preliminarily explored, and reference is provided for research on prevention and treatment of the visceral white-spot disease of the small yellow croakers.

The invention discloses a dCAPS molecular marker for identifying a wheat leaf rust resistance associated SNP site and application of the dCAPS molecular marker, and belongs to the technical field of wheat disease resistance breeding and molecular biology. The invention develops a dCAPS molecular marker of SNP (Single Nucleotide Polymorphism) linked with a leaf rust resistance genetic locus QLr.hebau-1BS in a wheat adult-plant stage by virtue of 277 parts of wheat materials which have genotype data and are introduced from Turkey and in combination with a Chinese spring reference sequence. By utilizing the molecular marker, different wheat can be accurately and quickly detected, early-stage molecular-assisted selection of leaf rust resistant germplasm materials in the adult-plant stage is realized, and the wheat disease-resistant breeding efficiency is improved. The method solves the problem of high difficulty in detecting the leaf rust resistance gene in the wheat adult-plant stage in the prior art, and is suitable for the field of molecular genetic breeding.