2163 results about "Disease resistant" patented technology

The present invention relates to the technical field of pesticides, and discloses a biological agent-based rice sheath blight disease efficient control agent, which comprises an active flora, a carrier, an auxiliary agent and a disease resistance enhancing factor, the active flora is used for combining humidity, pH and temperature response type materials so as to make the bacterial agent dynamically released, and the disease resistance enhancing factor is used for controlling the disease resistance of the rice sheath blight disease. The carrier is used for enabling the active ingredients to be slowly released in a field environment and keep stability, the auxiliary additive is used for improving the activity, adhesiveness and soil adaptability of the fungicide, and the disease-resistant enhancing factor is used for expanding and improving the adaptability of the control agent. The ratio of the active flora to the carrier to the auxiliary additive to the disease-resistant enhancing factor is 10: 50: 13: 27. Through the synergistic effect of various functional strains and the combination of the dynamic release carrier, the occurrence and propagation of the rice sheath blight disease are effectively inhibited, the prevention and treatment rate can reach 75-85%, and the prevention and treatment effect is obviously higher than that of a traditional chemical pesticide or a single fungicide.

The invention provides bacillus and application thereof, the bacillus is preserved in Guangdong Microbial Culture Collection Center on June 6, 2025, and the preservation number is GDMCC No.66483; one application of the bacillus is to prepare a preparation for promoting growth and / or resisting diseases of plants, and the other application is to use the bacillus for inhibiting microorganisms; the bacillus can effectively and enduringly inhibit the growth of phytopathogen, and can be used as a broad-spectrum antibacterial biological antibacterial agent to enhance the resistance of plants and promote the growth of the plants.

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

The invention discloses a wheat E3 ubiquitin ligase gene RFEL1 and application of the wheat E3 ubiquitin ligase gene RFEL1 in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The invention discloses a nucleotide sequence of a wheat E3 ubiquitin ligase gene RFEL1 as shown in SEQ ID NO. 3. The invention further provides application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof. The application comprises any one of the following applications: A1, improving the resistance of wheat to stripe rust; a2, the resistance of wheat to powdery mildew is improved; and A3, the resistance of wheat to leaf rust is improved. The E3 ubiquitin ligase RFEL1 gene provided by the invention not only has broad-spectrum resistance to different stripe rust, but also has resistance to wheat leaf rust and powdery mildew. The E3 ubiquitin ligase RFEL1 gene disclosed by the invention is applied to broad-spectrum resistance breeding of wheat and has important application value.

The invention belongs to the technical field of aquatic biotechnology and molecular marker-assisted breeding, and particularly relates to a cynoglossus semilaevis vibrio harveyi-resistant KASP molecular marker combination and application thereof in cynoglossus semilaevis disease-resistant breeding. The KASP molecular marker combination comprises three SNP (Single Nucleotide Polymorphism) loci which are respectively positioned at 17314099, 17325496 and 17355989 loci of the 14 # chromosome of the cynoglossus semilaevis, when the genotypes of the three SNP loci are respectively CC, AA and GG, the disease resistance of the cynoglossus semilaevis is high, and the invention also provides a primer for detecting the genotype polymorphism of the SNP loci. By utilizing the KASP molecular marker combination, the vibrio harveyi resistance genotype in a cynoglossus semilaevis breeding population can be quickly detected, and disease-resistant individuals are screened, so that the disease-resistant breeding efficiency of the cynoglossus semilaevis is greatly improved, and a new variety of the cynoglossus semilaevis with strong disease resistance can be quickly cultivated.

The invention provides a Kitasatospora xanthophycea JY01 strain and application thereof, the strain is separated from intestinal tracts of spring silkworms, has good disease-resistant and growth-promoting effects, and is specifically expressed in that cellulase, beta-1, 3-glucanase, siderophore and auxin IAA can be secreted; pot experiments show that the biocontrol efficiency of the microbial inoculum to wheat stalk rot is 57.90%, the biocontrol efficiency of the microbial inoculum to corn stalk rot is 46.41%, and the plant height and fresh weight are both significantly increased. The microbial agent is expected to be applied to preparation of biological bacterial fertilizer for effectively preventing and controlling soil-borne fungal diseases.

The invention provides a Ustilaginoidea virens gene UvASE2 for regulating and controlling rice resistance and application of the Ustilaginoidea virens gene UvASE2, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the Ustilaginoidea virens gene UvASE2 is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the Ustilaginoidea virens gene UvASE2 is as shown in SEQ ID NO: 2. By constructing a transgenic rice plant for heterologous expression of UvASE2, it is found that the transgenic rice can significantly improve the resistance to false smut and bacterial blight and significantly induce the outbreak of active oxygen. The heterologous expression UvASE2 gene of Ustilaginoidea virens has the function of positively regulating and controlling the disease resistance of rice, and the UvASE2 gene can be used for improving the disease resistance of rice and has important significance on creation of disease-resistant germplasm of rice.

The invention discloses a method for improving citrus yellow vein disease resistance by using a ClPsaN gene. The method comprises the following steps: (1) cloning a VIGS fragment of the citrus ClPsaN gene; (2) constructing two VIGS expression vectors of TRV and CLBV; and (3) transforming the citrus by the VIGS expression vector to obtain a citrus ClPsaN gene silenced VIGS plant. According to the invention, the VIGS vector of the Citrus ClPsaN gene is constructed, and then the Citrus is transformed, so that the obtained Citrus plant can show obvious resistance to the Citrus yellow vein clearing virus, the titer of the Citrus yellow vein clearing virus can be reduced by 22-34%, the accumulation amount of the virus protein is reduced by 35-50%, and the attack degree of the Citrus yellow vein clearing virus is obviously reduced; the TRV silencing of the citrus ClPsaN gene does not influence the phenotype of a citrus plant, and the citrus ClPsaN gene can be used as a candidate gene for citrus yellow vein disease resistance breeding together with a plurality of citrus yellow vein disease-resistant and susceptible genes by utilizing the cooperation of VIGS silencing, RNA interference, gene editing and other technologies.

The invention relates to a biological preparation for improving quality of weever, the biological preparation comprises an injection and an edible agent, the injection comprises chemically modified chemically synthesized single-stranded small RNA, Rev-erbalpha protein and Per1 protein, and the edible agent comprises Clock core peptide 1, Clock core peptide 2 and taurine. Skeletal muscle development, liver lipid metabolism and generation of cell inflammatory factors of the weever are influenced by adding and injecting the feed, so that the quality of the weever is improved, intramuscular fat deposition is promoted, the taste and flavor of fish meat are improved, the energy reserve of the weever can be improved, and the culture cycle is shortened. The improvement of cell inflammatory factors shows that the disease resistance of the weever is improved, the survival rate in the breeding process is further improved, and the use amount of antibiotics is reduced. The method provides an important theoretical basis for improving fish meat quality in China and promoting high-quality, efficient and healthy development of aquaculture, and has a wide market prospect.

Rosa chinensis gray mold is a main disease in the cultivation process of rosa chinensis, and seriously threatens the quality and yield of cut-flower rosa chinensis. In order to effectively cope with the disaster stress which influences the yield and quality of the Chinese rose, 192 pairs of SSR primers are used in the research to carry out PCR amplification on DNA of parents' Jinmai '' Beijing red 'and 6 randomly selected offspring. 35 pairs of primers show genetic difference between male and female parents. The 35 pairs of primers are further used for carrying out PCR amplification on filial generation DNA, detection is carried out through PAGE electrophoresis, and an electrophoretic band is converted into a 0, 1 matrix for genetic typing. The method comprises the following steps: selecting an optimal subset through correlation regression analysis, and finally successfully screening out three primers, namely, Geno-BC-1, Geno-BC-2 and Geno-BC-3, which are related to gray mold resistance. The discovery provides an important theoretical basis for molecular marker-assisted Chinese rose disease-resistant breeding, and is expected to promote early identification of disease-resistant plants, so that the breeding period is shortened, and the breeding efficiency is improved.

The invention discloses a seed priming method for improving early-stage disease resistance of pepper seeds, which belongs to the field of agricultural planting and comprises the following steps: S1, pre-cleaning: soaking the seeds with sodium hypochlorite and then cleaning the seeds with sterile deionized water; s2, pulse phosphorylation activation treatment: adding seeds and an activation solution into a pressure-resistant polypropylene container connected with a vacuum pipeline, sealing, vacuumizing to-0.08 MPa, maintaining for 5-10 minutes, then recovering to normal pressure, transferring to a constant-temperature oscillator, and carrying out phosphorylation activation treatment; s3, immune training treatment: transferring the seeds subjected to phosphorylation activation treatment to an initiation solution containing UV-inactivated phytophthora capsici spore suspension and H2O2, and carrying out intermittent ventilation treatment; s4, stabilizing treatment: transferring the seeds subjected to immune training treatment into an ascorbic acid solution for treatment, filtering, and drying in the shade to obtain the pepper seeds. According to the invention, the problem of immune response interruption caused by insufficient signal intensity and fast protein turnover when a simulated environment is used for excitation in the prior art is solved.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention belongs to the field of prevention and treatment of tilletia controversa Kuhn, and particularly relates to a tilletia controversa Kuhn effect protein g16561 and application thereof. An amino acid sequence of the Tilletia controversa Kuhn effect protein is shown as SEQ ID NO: 2. The effect protein plays an important role in the process of inhibiting plant defense reaction, and has an interaction relationship with a wheat protein in the process of infecting wheat by Tilletia controversa Kuhn. According to the invention, the pathogenesis of Tilletia controversa Kuhn can be deeply researched, and the excavation of wheat disease-resistant genes and the cultivation of wheat disease-resistant varieties can be promoted.

The invention relates to the technical field of teasel breeding, and discloses a teasel germplasm resource multi-character collaborative screening breeding method, which comprises the following steps: multi-omics data acquisition: extracting teasel germplasm leaf DNA (deoxyribonucleic acid), and capturing exon regions of disease resistance and metabolism related genes by adopting a targeted sequencing technology to obtain an SNP (single nucleotide polymorphism) marker matrix; the method comprises the following steps: collecting root extracts, detecting medicinal components through ultra-high performance liquid chromatography-mass spectrometry, and constructing a metabolite abundance matrix; measuring phenotype data of plant height, root length, root weight, root rot morbidity and survival rate under drought stress; key site and metabolite correlation analysis: constructing a Bayesian network containing SNP markers, metabolite and phenotypes by using a graph database, and carrying out cross validation through a leave-one-out method. The dipsacus asper germplasm resource multi-character collaborative screening breeding method aims at solving the problem that a traditional breeding method lacks systematic integration analysis of genomes and metabolic pathways in dipsacus asper multi-character collaborative improvement.

The invention belongs to the field of biological organic fertilizers, and discloses a special organic selenium-rich fertilizer for grapes as well as a preparation method and application thereof. The fertilizer is prepared from the following raw materials in percentage by mass: 0.05-0.2% of a selenium complex, 2-15% of a stabilizer, 15-25% of a microbial fermentation product, 0.5-2.0% of a natural plant extract and the balance of auxiliary materials, totaling 100%. The microbial fermentation product is microbial fermentation liquid obtained by fermenting bacillus tequilensis and cyanobacteria; the auxiliary material is obtained by mixing sodium alginate and maltodextrin; the natural plant extract is obtained by mixing azadirachtin, sophocarpidine, tea tree essential oil and cnidium lactone. Through the synergistic effect of the microbial fermentation product, the natural plant extract and the auxiliary materials, the industrial problem that in the prior art, a selenium fertilizer can only improve the selenium content of grapes, but cannot effectively improve the disease resistance and insect pest resistance of the grapes and slow down selenium element loss is solved, and the double improvement of the grape quality and the selenium content is achieved.

The invention relates to the technical field of microorganisms, in particular to a disease-resistant, growth-promoting and yield-increasing strain and a microbial agent and application thereof. The strain is a strain bacillus velezensis NB21 (CCTCC (China Center for Type Culture Collection) NO: M2024979) which is found autonomously; the strain can grow at low temperature, is strong in ecological environment adaptability, can be used for preparing a single microbial agent or a compound microbial agent, and can also be used for forming a composition with other functional organic and inorganic substances. The prepared microbial agent can significantly prevent and treat diseases, promote root development of crops such as rice and corn, enhance photosynthesis, improve system resistance, increase crop yield, enhance nutrient supply and improve soil micro-ecology. The strain is unique, strong in colonization ability and diversified in function, the prepared microbial agent fermentation production technology is mature, the cost is low, the application range is wide, the yield increasing and quality improving effects are outstanding, and the microbial agent has good application prospects.

The invention discloses protease for improving the resistance of potatoes to late blight as well as a coding gene and application thereof, and belongs to the technical field of genetic engineering and plant disease resistance breeding. The amino acid sequence of the protease StRD21 is as shown in SEQ ID NO.2, or the protease StRD21 has a derivative sequence with the same disease-resistant function. The nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. The invention also provides a specific primer pair for cloning the gene, a potato recombinant overexpression vector containing the gene and a transgenic host cell. The StRD21 gene is introduced into potatoes and overexpressed, so that the resistance of the potatoes to late blight can be remarkably enhanced. The invention provides an effective gene resource and a biotechnological means for cultivating a new variety of disease-resistant potatoes.

The invention relates to a microbial strain and application thereof, in particular to bacillus brevis, a microbial agent prepared from the bacillus brevis and application of the microbial agent. The invention provides the cold-resistant bacillus subtilis which has multiple functions of generating capsular polysaccharide, being high in salt resistance and cold resistance, promoting growth and disease resistance of plants in saline-alkali soil and the like. The bacterial strain can generate capsular polysaccharide, experiments prove that the bacterial strain has high capabilities of dissolving phosphorus, fixing nitrogen and producing siderophore, IAA, ACCD and cytokinin, and has strong antagonistic action on fusarium, curvularia and microplasmodium, and the generated capsular polysaccharide can promote salt tolerance of plants, so that the yield of the plants is increased, and the yield of the plants is increased. The bio-fertilizer is suitable for production of multifunctional bio-fertilizers with disease prevention, growth promotion, salt and alkali resistance, cold resistance and the like, is applied to production of saline-alkali soil grain crops, pasture and greenhouse commercial crops, has remarkable effects of promoting cold damage resistance, root development, prevention and control of root diseases, improvement of crop yield and the like, can reduce the use of chemical fertilizers and pesticides, and has a wide application prospect.

The invention discloses a preparation process of high-efficiency absorption fish protein peptide and application of the high-efficiency absorption fish protein peptide in fertilizer, and belongs to the technical field of marine organisms. The aquatic product protein is directly used as a fermentation raw material, and the content of amino acid in the fish protein biological peptide is remarkably increased by utilizing the synergistic fermentation effect of microorganisms and assisted by biological enzymolysis, so that the absorptivity of the fish protein peptide is increased; the prepared fish protein peptide is used in the fertilizer to prepare the fish protein peptide functional fertilizer, the nutritional value and the utilization rate of the fertilizer are remarkably improved, and the fish protein peptide functional fertilizer has the remarkable effects of growth promotion, disease resistance, stress resistance and the like. According to the method, a conventional fish protein peptide preparation method of firstly preparing fermentation enzyme liquid and then performing biological enzymolysis is abandoned, the efficient absorption fish protein peptide is prepared by adopting a process of directly fermenting aquatic protein, a fermentation process and an enzymolysis process are integrated, and compared with a conventional process, the method has the advantages of simple production process, low production cost and the like; the method has significant effects and meanings for promoting green preparation of the fish protein peptide and efficient application of the fish protein peptide in fertilizers.

According to the microbial composite soil conditioner and the preparation method thereof, decomposition of organic matters in soil can be promoted through multiple microorganisms in the microbial composite soil conditioner, and the air permeability and the water-retaining property of the soil are improved. Meanwhile, the zeolite powder and the rice hull powder ash can improve the pore structure of the soil and improve the drainage capacity and the air permeability of the soil, so that the growth environment of the plant root system is optimized. The decomposed organic fertilizer and the edible fungus residue organic matter are rich in nutrient elements of nitrogen, phosphorus and potassium, and can provide continuous and stable nutrient supply for crops. In addition, the amino acid stock solution, the pyroligneous liquor and the molasses liquor contain various trace elements and bioactive substances, so that the disease resistance and the growth vitality of crops can be enhanced. Due to the improvement of the soil structure, the improvement of fertility and the enhancement of microbial activity, crops can better absorb nutrients and moisture, the occurrence of diseases and pests is reduced, and the increase of the crop yield and the crop quality are effectively improved.

This invention discloses a microbial inoculant for controlling peanut root rot and its preparation method, belonging to the field of microbial materials technology. This invention screened and obtained a *Trichoderma harzianum* strain with highly efficient inhibitory effects against the pathogen causing peanut root rot. Firstly, the strain itself exhibits antagonistic activity against the peanut pathogen. Secondly, it was prepared as a spore suspension and fermented in solid-state with rice husks and wheat bran. During the solid-state fermentation process, the metabolites produced by *Trichoderma harzianum* promote the growth and development of plant roots, increasing plant biomass and yield. This microbial inoculant effectively controls peanut root rot, exhibiting good inhibitory effects against the pathogen, thus improving peanut quality and disease resistance. Furthermore, it improves soil ecological activity to a certain extent, making it worthy of application and promotion.

The invention belongs to the technical field of fertilizers, and particularly relates to a fertilizer for capsicum as well as a preparation method and a fertilization method thereof. In the pepper planting process, the proportion of carbon and nitrogen in the soil is coordinated, the nutrient slow release effect is achieved, the contradiction between vegetative growth and reproductive growth of pepper plants is relieved, and the fertilizer utilization rate is increased. In addition, a microbial agent is applied to the roots after transplanting, and the soil matrix and the disease resistance are enhanced by utilizing the disease resistance and the soil microenvironment improving capability of the microbial agent, so that the pepper yield is increased. The technology is simple and easy to implement, convenient to operate and quick in effect, common farmers or farmers can easily implement the technology, soil fertility and disease resistance can be continuously improved, and the problem of nitrogen and phosphorus loss of the peppers is effectively solved.

The invention belongs to the technical field of biological control, and particularly relates to the technical field of crop stem rot control. The invention provides a bacillus velezensis Gc8, and the preservation number of the bacillus velezensis Gc8 is CGMCC (China General Microbiological Culture Collection Center) No. 28991. The bacillus velezensis Gc8 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 14, 2023, the preservation number of the bacillus velezensis Gc8 is CGMCC No.28991, the center is called CGMCC for short, and the address of the center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides a compound microbial agent, wherein the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67, kitasapora fluorescens pf27, kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27. The compound microbial agent can inhibit spore germination of pathogenic microorganisms, and has the effect of promoting growth of corn while resisting diseases.

The invention relates to the technical field of gene isolation and plant improvement, in particular to a late blight resistance gene, a biological material and application. The Rpi-card1 nucleic acid molecule provided by the invention comprises a nucleotide sequence as shown in any one of SEQ ID NO: 1-4; a nucleotide sequence encoding the amino acid sequence represented by SEQ ID NO: 5 or 6; the nucleotide sequence has at least 75% sequence identity with the nucleotide sequence. The invention provides an important gene resource for potato late blight resistance breeding, overcomes the problem of disease resistance loss of potato varieties, and provides a new guarantee for lasting disease resistance of potatoes.

The invention discloses a special fertilizer composition for high-yield cultivation of sugarcane, and belongs to the technical field of sugarcane planting. Comprising a disease-resistant fertilizer and an insect-resistant fertilizer; wherein the disease-resistant fertilizer comprises the following raw materials in parts by mass: 50 parts of diatomite, 5-10 parts of biomass charcoal, 1-2 parts of a compound microbial agent, 150-170 parts of an organic fertilizer and 50-60 parts of an inorganic fertilizer; wherein the compound microorganism bacterium agent comprises a bacillus subtilis bacterium agent, a trichoderma harzianum bacterium agent, a trichoderma viride bacterium agent and a paecilomyces lilacinus bacterium agent; the insect prevention fertilizer comprises beauveria bassiana suspension and matrine in a volume ratio of (3.5-4.5): 1. According to the fertilizer composition special for high-yield cultivation of sugarcane, the synergistic effect of microorganisms is utilized, the broad-spectrum inhibition effect on microorganisms in sugarcane continuous cropping disease soil can be achieved, the kieselguhr and the biomass charcoal cooperate to form a porous substance, nutrient elements are adsorbed in pores, the slow-release effect is improved, the fertilizer efficiency is prolonged, and the effect is good. Moreover, the soil structure can be improved, and the disease resistance of the sugarcane during continuous cropping is greatly improved through the synergistic interaction with the microbial complex microbial inoculants.

The utility model relates to the technical field of screening devices, and discloses a raw material screening device for processing disease and insect resistant pesticide fertilizer, which comprises a bottom frame, a vibration screening machine for disease and insect resistant pesticide fertilizer raw materials is fixedly mounted on the bottom frame, a material guide device is arranged on the vibration screening machine, and a feeding component is mounted on the bottom frame; the feeding assembly comprises a door-shaped frame, a hopper is fixedly installed on the top of the door-shaped frame, a discharging pipe is fixedly connected to the lower portion of the hopper, a material distributing barrel is rotationally connected to the discharging pipe, a material distributing groove is formed in the material distributing barrel, a speed-adjustable motor is fixedly installed on the discharging pipe, and the motor is connected with the material distributing barrel through an output shaft. The material distributor comprises an L-shaped material guiding pipe, connecting plates are fixedly installed on the two sides of the L-shaped material guiding pipe, the lower portions of the connecting plates are fixedly installed on the vibration screening machine, and a plurality of material distributing protrusions are fixedly connected to the bottom, close to the discharging opening, of an inner cavity of the L-shaped material guiding pipe. According to the utility model, the feeding of the screen can be accurately controlled, and meanwhile, the disease-resistant and insect-resistant pesticide-fertilizer raw materials can be uniformly dispersed, so that the screening efficiency is improved.

The application discloses an NLR plug-in gene for improving plant disease resistance and application thereof, and belongs to the field of plant molecular biology and biotechnology.The NLR plug-in gene StEM1 has a whole gene sequence as shown in SEQ ID NO.1 and a CDS sequence as shown in SEQ ID NO.2, and the amino acid sequence of the encoded protein is as shown in SEQ ID NO.3.The gene plays a key role in improving plant disease resistance, especially resistance to field late blight variant bacteria.In the NLR background, overexpression of the gene significantly promotes the disease resistance of tobacco and potato to pathogenic pythium, and the gene is a gene for enhancing plant disease resistance by improving ETI immunity.Overexpression of the gene in the NLR background is obtained by a genetic transformation method, and a strain line is obtained to resist field variant strains, so as to cope with field diseases of crops.The application provides an excellent gene resource for green prevention and control of late blight and disease-resistant genetic engineering breeding.

The invention discloses application of an ESP1 gene in regulating the resistance of plants to rice blast, and belongs to the technical field of molecular biology. The invention provides the application of the ESP1 gene in regulating the resistance of the plant to the rice blast, and experiments prove that a mutant plant with a silent ESP1 gene is susceptible to the rice blast, and a mutant plant with an overexpressed ESP1 gene is resistant to the rice blast. The invention is expected to provide a good theoretical basis for cultivating disease-resistant plants.

The invention belongs to the technical field of genetic engineering, and particularly relates to a CsECT protein related to plant disease resistance, a recombinant vector and application of the CsECT protein. The invention provides a CsECT protein related to plant disease resistance, the CsECT protein is synthesized by gene CsECT coding, it is speculated that the gene CsECT is used as an m6A reader, metabolism of target mRNA of the gene CsECT is affected, the stability of disease resistance related target genes is improved, expression of disease resistance genes is promoted, and then the disease resistance of plants is improved. The result of the embodiment shows that after the CsECT gene is cloned, the CsECT transgenic citrus is constructed, the CsECT gene is excessively expressed by the transgenic citrus, the canker resistance of the citrus can be remarkably improved, and then the disease resistance of the citrus is improved. Therefore, the CsECT protein synthesized by the gene CsECT disclosed by the invention can be used for improving the disease resistance of plants.

The present invention belongs to the technical field of biological prevention and treatment for viral diseases. Disclosed in the present invention are a tomato disease-resistant gene mutant, and the use thereof in the prevention and treatment of ToBRFV. It is found in the present invention that mutating the nucleotide at position 1927 of the coding region sequence of tomato Tm-22 gene from G to A, or mutating the amino acid at position 643 of the LRR domain of a protein that is encoded by tomato Tm-22 gene from glycine to arginine can remarkably reduce the accumulation level of ToBRFV capsid protein (CP). The gene (named Tm-22-Mut5) can serve as a novel ToBRFV resistant gene, and also retains the resistance to TMV, ToMV and ToMMV. The Tm-22-Mut5 and a pre-screened Tm-22-Mut3-1 mutant (tyrosine at position 767 of the LRR domain thereof is mutated to phenylalanine) undergo combinatorial mutagenesis to obtain a mutant Tm-22-Mut6. Analysis shows that the Tm-22-Mut6 can remarkably reduce the accumulation level of the ToBRFV capsid protein. Compared with the pFGCTm-22-Mut3-1 mutant obtained by screening in the previous research and the newly obtained mutant Tm-22-Mut5, the Tm-22-Mut6 has further improved resistance to ToBRFV.