13 results about "Chaetomium globosum" patented technology

The present application belongs to the field of biotechnology, and particularly relates to a preparation method and application of five compounds (1-5) extracted from liquid fermentation of Chaetomium sp. Specifically, the present application discloses five compounds, wherein compound 1 is a new compound. The Chaetomium sp. metabolite has good antibacterial activity on three tested pathogenic bacteria (Pseudomonas syringae pv. actinidiae, Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola), and has important significance for development of a new type of microbial source antibacterial agent.

This invention belongs to the field of biological control technology for plant diseases and pests, and provides a strain of Chaetomium globosum ( Chaetomium globosum EF-14 and its applications. This strain is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 40618. The *Chaetomium globosum* EF-14 provided by this invention is an endophytic fungus of maize that can simultaneously inhibit *Fusarium graminearum* (…). Fusarium gramineae ) and Corn spores ( Bipolaris maydis It can effectively reduce corn stalk rot and corn leaf spot caused by fall armyworm ( ) and can also effectively reduce the incidence of fall armyworm ( ). Spodoptera frugiperda This invention provides a highly efficient and safe microbial resource for the green control of major corn diseases and pests, and broadens the application scope of Chaetomium globosum in agricultural biocontrol, achieving synergistic control of diseases and pests through "one fungus targeting multiple targets".

The invention belongs to the technical field of agricultural microorganisms, and relates to a biocontrol fungus for preventing and treating pomegranate fusarium wilt, a biocontrol microbial inoculum and application of the biocontrol fungus and the biocontrol microbial inoculum. According to the invention, a chaetomium globosum N9 strain is separated and screened from microbial resources on pomegranate branches, the chaetomium globosum N9 strain is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO: 67114. The N9 strain provided by the invention has a good antagonistic effect on the main pathogenic bacterium-sweet potato long-beak-shell fungus causing pomegranate fusarium wilt, and can significantly inhibit the growth of sweet potato long-beak-shell fungus hyphae, and the inhibition rate is up to 75.9%. The biocontrol fungus and the biocontrol inoculant provided by the invention have application prospects in the aspect of preventing and treating plant diseases caused by sweet potato coracocystis longirostris, especially in the aspect of preventing and treating pomegranate fusarium wilt.

The invention discloses a symbiotic system of monolith and chaetomium globosum as well as a construction method and application of the symbiotic system. According to the invention, the multifunctional symbiont of the Coelasstra sp. And the Chaetomium globosum is successfully constructed by virtue of a BG11 inorganic salt culture medium, and the multifunctional symbiont of the Coelasstra sp. And the Chaetomium globosum is constructed by virtue of the BG11 inorganic salt culture medium. The symbiont has the effects of promoting growth of crops, relieving salt stress of the crops, degrading straws and inhibiting fusarium graminearum, and chaetomium globosum and monolith in the symbiont can be planted around root systems of the crops. The algal-bacterial symbiont not only realizes maximum utilization of resources, but also provides powerful support for green and healthy development of agriculture.

The invention discloses a chaetomium globosum sourced ergothioneine synthetase CgEgt2 and a coding gene cgegt2 of the chaetomium globosum sourced ergothioneine synthetase CgEgt2. The amino acid sequence and the nucleotide sequence of the chaetomium globosum sourced ergothioneine synthetase CgEgt2 are shown as SEQ ID NO.2 and SEQ ID NO.1. The invention further discloses a preparation method of the The invention provides a Kluyveromyces marxianus recombinant strain KMNC-WZ02 containing a cgegt2 gene and an ncegt1 gene, the preservation number of the Kluyveromyces marxianus recombinant strain KMNC-WZ02 is CGMCC (China General Microbiological Culture Collection Center) NO.29599, a two-copy strain of the KMNC-WZ02 is constructed, and the ergothioneine yields of the KMNC-WZ02 and the two-copy strain of the KMNC-WZ02 respectively reach 466 mg / L and 590.3 mg / L after the KMNC-WZ02 and the two-copy strain of the KMNC-WZ02 are fermented for 7 days. The method provided by the invention is of great significance to excavation of EGT synthetases from different sources and construction of efficient EGT production strains.

The invention belongs to the technical field of application of chaetomium globosum, and particularly relates to chaetomium globosum Ba08, a solid-state fermentation biological agent and application of the chaetomium globosum Ba08, and the preservation number of the chaetomium globosum Ba08 is CCTCC M 2025292; the solid state fermentation biological agent comprises chaetomium globosum Ba08 or metabolite; the invention discloses application of a solid-state fermentation biological agent in in-situ remediation of organic contaminated soil. The organic contaminated soil comprises polycyclic aromatic hydrocarbon contaminated soil, chlorine-containing organic contaminated soil and petroleum contaminated soil. The chaetomium globosum Ba08 can secrete cytochrome enzyme P450, manganese peroxidase and laccase under the aerobic condition, and C-C conjugated double bonds of aromatic hydrocarbon are broken through enzyme action, so that physiological toxicity of polycyclic aromatic hydrocarbon metabolism intermediate products is relieved, benzene series, heterocyclic compounds and polycyclic aromatic hydrocarbon can be thoroughly mineralized, and the yield of the product is increased. And the utilization rate of the petroleum biodegradation residues is obviously improved.

This invention belongs to the field of biocontrol microbial technology, specifically relating to a strain of Chaetomium globosum (… Chaetomium globosum )5141-4 、 Inoculants and their applications. This invention isolates an endophytic fungus from *Lithocarpus* lichens and names it *Chaetoceros globosa*. Chaetomium globosum 5141-4. Verification has shown that the above strains can effectively inhibit the growth of Fusarium oxysporum or anthrax, and also exhibit good inhibitory effects against six pathogens, including Pseudomonas aeruginosa and Enterococcus faecalis. They hold promise for use in the preparation of biocontrol products or further screening of antibacterial compounds.

This invention discloses a method for preparing recombinant dextranase from Chaetomium globosum and its uses. The amino acid sequence of the recombinant dextranase is shown in SEQ ID NO.2 or SEQ ID NO.4, and the nucleotide sequence of its encoding gene is shown in SEQ ID NO.1 or SEQ ID NO.3. The optimal pH of the recombinant dextranase is 4.5-7.0, and the optimal reaction temperature is 45-60℃. + K + and Ca 2+ It has an activating effect on enzyme activity, Mg 2+ NH4 + and Mn 2+ It had no significant effect on enzyme activity, Fe 3+ Zn 2+ Cu 2+ and Co 2+ It has an inhibitory effect on enzyme activity. Furthermore, this enzyme exhibits excellent pH stability, good thermal stability, and anti-interference ability, making it well-suited to the application requirements of the food, pharmaceutical, and chemical industries.

The invention relates to the technical field of microorganisms, in particular to a culture method of chaetomium globosum strain H-1 with high yield of uranidin, which comprises the following steps: (1) activating the strain; (2) preparing an optimized culture medium which comprises 20-30 g / L of glucose, 5-9 g / L of fish peptone, 0.1-0.5 g / L of dipotassium phosphate and the balance of deionized water, and adjusting the initial pH value of the culture medium to 6.5-7.5; (3) inoculating the activated strain into the optimized culture medium, and standing and culturing for 8-12 days under the dark condition of 23-27 DEG C; and (4) after the culture is finished, separating and extracting the yellow pigment. By accurately optimizing the carbon source, the nitrogen source, the inorganic salt concentration and culture environment parameters, the yield of the yellow pigment of the chaetomium globosum is remarkably increased, the produced yellow pigment is a natural water-soluble pigment and is high in biological safety, and the culture method is easy and convenient to operate, controllable in cost, good in repeatability, suitable for industrial large-scale production and suitable for industrial large-scale production. And an effective technical support is provided for industrial application of the natural pigment.

This invention discloses a symbiotic system of *Algae monostellatum* and *Chaetomium globosum*, its construction method, and its application. This invention successfully constructed *Algae monostellatum* (…) using BG11 inorganic salt medium. Coelastrella sp.) and Chaetoceros (sp.) and Chaetoceros globulus ( Chaetomium globosum This multifunctional symbiotic organism promotes crop growth, alleviates crop salt stress, degrades straw, and inhibits Fusarium graminearum. Furthermore, both Chaetomium globosum and Algae moniliforme within the symbiotic organism can colonize around the crop root system. This fungal-algae symbiotic not only maximizes resource utilization but also provides strong support for the green and healthy development of agriculture.

This utility model belongs to the technical field of microbial fermentation and spore extraction system equipment, specifically relating to a sterile material handling device for a *Chaetomium globosum* solid-state fermentation tank. It includes a material handling rod handle, a silicone corrugated tube, and a storage tube connected in sequence, and a material handling rod that passes through the internal cavities of the silicone corrugated tube and the storage tube. One end of the material handling rod is fixedly connected to the handle, and the other end is equipped with a material handling ring. This utility model achieves sealed material handling through the coordinated action of the material handling rod handle, the silicone corrugated tube, the storage tube, and the material handling rod, ensuring no leakage or contamination during material transfer. The silicone corrugated tube, due to its elasticity, fits tightly against the material handling rod, forming a dynamic seal. The elasticity of the silicone corrugated tube allows the material handling rod to move back and forth while maintaining a seal, providing a sterile environment for the rod's movement.

The application provides a chaetomium globosum, a method for separating secondary metabolites of the chaetomium globosum and application, and belongs to the technical field of biological medicines. Arcopilus cupreus DGR-7 has been preserved in Guangdong Microbial Culture Collection Center on October 10, 2025, with a preservation number of GDMCC No: 67068; the application first utilizes rice medium to ferment A. cupreus strains, and 8 compounds are separated and purified from the strains, wherein 6 compounds are anthraquinones. In-vitro antibacterial experiments show that the chaetomium globosum Arcopilus cupreus The secondary metabolites of DGR-7, anthraquinones, have good bioactivity in inhibiting the growth of staphylococcus aureus, methicillin-resistant staphylococcus aureus, streptococcus salivarius, klebsiella pneumoniae, bauman acinetobacter, pseudomonas aeruginosa and escherichia coli.

The invention provides chaetomium hordeum, a method for separating secondary metabolites from chaetomium hordeum and application of the chaetomium hordeum, and belongs to the technical field of biological medicine. The chaetomium cupreum DGR-7 is preserved in the Guangdong Microbial Culture Collection Center on October 10, 2025, and the preservation number of the chaetomium cupreum DGR-7 is GDMCC No: 67068; according to the invention, the rice culture medium is used for fermenting the A.cupreus strain for the first time, and the A.cupreus strain is separated and purified to obtain 8 compounds, including 6 anthraquinone compounds. An in-vitro antibacterial experiment shows that the secondary metabolite anthraquinone compound of the Arcoporus cupreus DGR-7 has good biological activity in the aspect of inhibiting the growth of staphylococcus aureus, methoxyl-resistant staphylococcus aureus, streptococcus salivarius, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa and escherichia coli.