73 results about "Chaetomium globosum" patented technology

The invention discloses a chaetomium globosum FSR-74. The collection unit of the chaetomium globosum FSR-74 is China General Microbiological Culture Collection Center, the collection date of the chaetomium globosum FSR-74 is September 19, 2014 and the collection number of the chaetomium globosum FSR-74 is CGMCC No. 9690. The strain is relatively slow in growth and has light brown colonies and an irregular wave-like edge when subjected to plate culture at 25 DEG C in a potato dextrose agar (PDA) culture medium. FSR-74 ascocarps are supergene and fixed on the surface of a matrix by rhizoids; top hair is wavy, brown and has intervals, and dense wart points are arranged on the surface; 8 ascospores are arranged in an ascus; the ascospores are shaped like lemons and have single apical bud holes. The chaetomium globosum FSR-74 has an efficient broad-spectrum bacteriostatic effect against main pathogenic bacteria causing ginseng rust rot, blight, sclerotinia rot, rust rot, blackspot, sheath blight and gray mold. The invention further provides a biological control mechanism and application of the chaetomium globosum FSR-74 in prevention and treatment of plant fungal diseases, as well as application in preparation of a microbial agent for preventing and treating the plant fungal diseases.

The invention belongs to the technical field of microbe application and discloses a microbial agent for promoting quick decomposition of agricultural wastes, prepared from the following steps of respectively carrying out first stage amplification culture and second stage amplification culture on four microbes which are bacillus subtilis, micromonospora streptomyces, trichoderma koningii and chaetomium globosum, and respectively obtaining four liquid cultures of the microbes; then commonly inoculating the four liquid cultures in a carrier culture medium according to a ratio with the inoculation amount of 0.2 percent, controlling the moisture content at 55-60 percent, culturing for 7 days at a constant temperature of 28 DEG C; and finally, carrying out low-temperature drying treatment on a complex bacteria subjected to culturing at the temperature of 35-40 DEG C, and carrying out live bacteria detection. The microorganism composting microbial agent can promote quick decomposition of agricultural waste aerobic composts, has remarkable action of eliminating malodorous gas generated by organic wastes and also reduces the nutrient loss caused by volatilization in a composting process.

The invention relates to the technical field of microorganisms, and in particular, relates to salviae miltiorrhizae endophytic fungi which are separated from labiatae salvia plant salviae miltiorrhizae plant living bodies by an endophytic fungi separation and purification technology, are classified and named as chaetomium globosum D68 and have the preservation number of CGMCC No.12622. The invention further discloses an application of the salviae miltiorrhizae endophytic fungi in improvement of the host salvia miltiorrhiza biomass and the content of tanshinones and phenolic acids. The salvia endophytic fungi can effectively improve the salvia miltiorrhiza root biomass through co-culture with salvia miltiorrhiza plants, significantly increase the plant crown breadth, height and single-leaf area, also can effectively promote biological synthesis of tanshinones, phenolic acids and other effective components in salvia miltiorrhiza, and have great application value.

The invention discloses chaetomium globosum with strong inhibition effect agaisnt anthracnose pathogens of apples. The chaetomium globosum is registered and booked with the number being CGMCC No. 10609 in general microorganism center of China committee for culture collection of microorganisms, and the collection date is march 11th, 2015. The chaetomium globosum HY1 disclosed by the invention is endophytic fungi of glehnia littoralis as a threatened plant, both fermentation liquor and mycelium extracting solution of the chaetomium globosum have strong inhibition effect against the anthracnose pathogens of the apples, and also have certain inhibition action against other plant pathogens, and the strain has wide application prospect in the aspects of preventing and treating the anthracnose pathogens of the apples and the like.

Provided are a bacterial strain and a microbial agent for preventing and curing apple tree rotten diseases and a preparation method of the microbial agent. The strain 61239 is preserved in China Center for Type Culture Collection which is called CCTCC for short on July 1st, 2015, and the preservation number is CCTCC M2015420. The invention further comprises the microbial agent containing chaetomium globosum for preventing and curing the apple tree rotten diseases and the preparation method of the microbial agent. Biological control fungi come from the natural world, pollution, public hazards and residues do not exist, toxic and side effects on people and livestock do not exist, the safety is achieved for apple trees, human bodies and ecological environments, the biological control fungi can be applied to any growth period of the apple trees, specially a dormant period, no resistance is produced, and the apple rotten diseases can be thoroughly controlled and radically cured. It is proved through experiments that specificity of the chaetomium globosum strain 61239 to apple tree rotten germs is high, the control effect is good, and the average control effect is 95 percent or above.

The invention relates to an application of a strain of gingko endogeny Chaetomium globosum NO.04 in prevention and control of plant pathogenic fungi, and belongs to the technical field of biological prevention and control of plant diseases. A strain of the gingko endogeny Chaetomium globosum NO.04 and fermentation metabolites thereof in the present invention can be applied for prevention and control of northern corn leaf blight, Grapevine white rot, and tomato rhizopus fruit rot caused by plant pathogenic fungi. According to the present invention, a microbe strain is provided for prevention and control of northern corn leaf blight, Grapevine white rot, tomato rhizopus fruit rot, and other plant pathogenic fungi diseases on agriculture; and the provided fermentation metabolites can be applied in prevention and control of plant fungi diseases, and a new approach is provided for development of agricultural fungicides.

Novel tetramic acid-type compounds isolated from a CCR-5 active complex produced by fermentation under controlled conditions of a biologically pure culture of the microorganism, Chaetomium globosum Kunze SCH 1705, ATCC 74489., pharmaceutical compositions containing the compounds and the use of the CCR-5 antagonist compounds and compositions to treat HIV-1 infections in humans are disclosed.

The invention relates to biocontrol bacterial strain B221 which can prevent the verticillum wilt of cottons, and the biocontrol bacterial strain B221 belongs to the technical field of the agricultural crop. The biocontrol bacterial strain B221 is identified as the chaetomium globosum. The preservation number of the strain is CGMCC NO.2493. The strain is shaken and cultured in PDA culture solution with 25 DEG C 200rpm for 7 days and is filtered through eight-layer of sterilization gauze to obtain the spore suspending liquid, the total concentration of the viable bacteria in the finished product is 10 <9> spores / milliliters, therefore to obtain the biocontrol bacterial agent. The bacterial strain is preserved in the PDA inclined surface at 4 DEG C, the bacterial strain B221 represents better flat plate antagonism effect, greenhouse prevention effect and growth facilitation effect. The experiment shows that the greenhouse prevention effect of the bacterial strain B221 achieves 58.96 percent, the biomass increases by 51.20 percent; the biocontrol bacterial strain B221 has greater potentiality to be developed into the commercialized viable bacteria biocontrol agent.

The invention discloses a preparation method of a special syzygium samarangense pesticide fertilizer. The special syzygium samarangense pesticide fertilizer is prepared by taking cow dung, chicken manure, straws, bean cakes, bagasse, urea, calcium superphosphate, potassium sulfate, trace element, composite amino acid, traditional herbal medicine extracting solution, bacillus subtilis, chaetomium globosum and the like in parts by weight as raw materials through fermenting, crushing, mixing and the like. The pesticide fertilizer produced by the invention contains a beneficial microbial bacterial colony and Chinese herbal medicine components, can be used for preventing diseases and pests, and does not pollute fruits.

The invention discloses composite microbial organic bacterial fertilizer capable of improving salt resistance of seedlings. The composite microbial organic bacterial fertilizer is prepared from the following raw materials in parts by weight: 10 to 19 parts of potatoes, 1 to 4 parts of brown sugar, 16 to 24 parts of chaetomium globosum fermented liquid, 1 to 4 parts of disodium hydrogen phosphate, 1 to 4 parts of calcium chloride, an appropriate amount of 0.1 mol / L sodium chloride solution, an appropriate amount of 0.2 mol / L acetate solution, 23 to 31 parts of chitosan, 2 to 6 parts of glutaraldehyde, 12 to 19 parts of wheat bran, 12 to 18 parts of furfural residue, 6 to 11 parts of ammonium sulfate, 7 to 13 parts of potassium sulfate, 16 to 23 parts of manure and an appropriate amount of deionized water. According to the composite microbial organic bacterial fertilizer, the chaetomium globosum is cultured in a fermenting manner, then the chitosan is used for wrapping the chaetomium globosum fungicide to enable the chaetomium globosum fungicide to be slowly released, by virtue of microbial secondary fermentation treatment, the demand of crops on various nutrients can be satisfied, the growth of the chaetomium globosum strain can be satisfied and promoted, the soil to which the fertilizer is applied can continuously accumulate the nutrients and improve the insecticide and anti-disease capacity, and the salt resistance of the crop seedlings can be obviously improved.

The invention relates to an application of a strain of gingko endogeny Chaetomium globosum NO.05 in prevention and control of plant pathogenic fungi, and belongs to the technical field of biological prevention and control of plant diseases. A strain of the gingko endogeny Chaetomium globosum NO.04 and fermentation metabolites thereof in the present invention can be applied for prevention and control of northern corn leaf blight, Grapevine white rot, and tomato rhizopus fruit rot caused by plant pathogenic fungi. According to the present invention, a microbe strain is provided for prevention and control of northern corn leaf blight, Grapevine white rot, tomato rhizopus fruit rot, and other plant pathogenic fungi diseases on agriculture; and the provided fermentation metabolites can be applied in prevention and control of plant fungi diseases, and a new approach is provided for development of agricultural fungicides.

The invention discloses a strain of a Dongxiang wild rice endophytic fungus for producing GAMG (glycyrrhetinic acid mono-glucuronide) by efficiently converting glycyrrhizic acid. The classification and nomenclature of the Dongxiang wild rice endophytic fungus is Chaetomium globosum DX-THS3, is collected in CCTCC (China Center for Type Culture Collection) and has the preservation number of CCTCC NO: M 2016005. By using the glycyrrhizic acid (glycyrrhetate or coarse liquorice root extracts) as carbon sources; the strain can be induced to generate beta-D-glucuronidase; further, the remote end glucuronyl in glycyrrhizic acid molecules is hydrolyzed; the GAMG is produced. The Chaetomium globosum DX-THS3 and the produced beta-D-glucuronidase can be used for producing the GAMG by converting the glycyrrhizic acid. The characteristics of single products, high conversion rate and the like are realized.

The invention relates to a preparation method of an antibacterial fermentation liquid of Solidago canadesis endophytic fungi, which comprises the following operation steps: 1. activating strain; 2. carrying out first-stage seed culture; 3. carrying out second-stage seed culture; and 4. carrying out fermentation culture to obtain brown Solidago canadesis endophytic fungus fermentation liquid, filtering the Solidago canadesis endophytic fungus fermentation liquid in aseptic condition to remove mycelia, and then passing a 0.22 mu m filter to obtain the antibacterial fermentation liquid of Solidago canadesis endophytic fungi. According to the invention, the preparation method of antibacterial fermentation liquid of Chaetomium globosum yt03 with broad spectrum antibacterial activity is determined; and antibacterial test finds that the Chaetomium globosum yt03 antibacterial fermentation liquid has relatively significant hypha growth inhibiting action on six tested plant pathogenic fungi.

The invention discloses a special medical fertilizer for the wax apple. The special medical fertilizer for the wax apple comprises the following raw materials: 18-22 parts of cow manure, 15-18 parts of chicken manure, 20-40 parts of straw, 10-20 parts of bean cake, 10-20 parts of bagasse, 40-50 parts of urea, 30-40 parts of calcium superphosphate, 30-40 parts of potassium sulphate, 2-5 parts of microelement, 3-5 parts of compound amino acid, 15-25 parts of Chinese herbal medicine extracting solution, 1-2 parts of bacillus subtilis and 0.6-1 parts of chaetomium globosum. The beneficial microorganism bacterial colony and the Chinese herbal medicine component are contained in the medical fertilizer so that the insect diseases can be prevented and the fruits cannot be polluted.

The invention discloses a microbial agent for preventing and controlling quarantine fungus diseases of apples and promoting yield increase. The microbial agent is prepared from raw materials in parts by weight as follows: 10-15 parts of streptomyces microflavus, 10-15 parts of bacillus subtilis, 15-25 parts of chaetomium globosum, 8-10 parts of bacillus laterosporus, 15-20 parts of bacillus amyloliquefaciens, 8-10 parts of bacillus circulans, 10-20 parts of trichoderma harzianum, 5-10 parts of trichoderma asperellum, 5-10 parts of trichoderma fasciculatum bissett, 5-10 parts of paecilomyces lilacinus, 8-12 parts of streptomyces syringini, 8-12 parts of gordonia, 8-12 parts of conexibacter, 5-10 parts of alga oligosaccharide and 10-15 parts of chitosan oligosaccharide citrate. The microbial agent can effectively inhibit the quarantine fungus diseases and can strengthen apple trees, and the yield increase purpose can be achieved; chaetomium globosum has remarkable bactericidal and bacteriostatic activity for valsa ceratosperma of the apple trees; no pesticides are used, the microbial agent is non-toxic, harmless, pollution-free and residue-free, the quality and the safety of agricultural products are guaranteed, and the apples have pure taste and high sweetness.

The invention provides chaetomium globosum (Chaetomium globosum), which has a preservation name of 12XP1-2-3 and a preservation number of CGMCC No.17183, wherein the preservation unit is the China general microbiological culture collection centre; and the preservation date is January 23th, 2019. The invention further provides application of chaetomium globosum in promoting growth and increasing yield of wheat. Field test results show that: when the chaetomium globosum 12XP1-2-3 provided by the invention is used for wheat seed coating treatment, the chlorophyll content of leaves can be increased by 1.9%-14.7% in the booting stage; and the wheat yield can be increased by 2.3%-10.9%.

The invention belongs to the technical field of medicine, and provides activities and structure of new indole alkaloid compounds 1-10 derived from insect symbiotic bacterium Chaetomium globosum secondary metabolite and also provides a separation purification preparation method and related activities of compounds pillworm chaetoglobosins E and H with favorable antitumor activity. The structural formula is disclosed as (I). The invention provides an alternative compound for developing new antitumor drugs, and has very important meanings for development and utilization of medicinal insect symbiotic bacterium resources.

The invention belongs to the technical field of medicine, and relates to a sowbug symbiotic bacterium of chaetomium globosum and application thereof to the preparation of indole alkaloid compounds with the antitumor effects. The invention provides activity and structures of new indole alkaloid compounds 1 to 10 from the secondary metabolites of the chaetomium globosum, and provides a separation purification preparation method and structure identification of the new compounds. The chaetomium globosum provided by the invention is preserved in CCTCC (China Center for Type Culture Collection), andhas the strain preservation number being CCCC M2016343. The preservation time is June 23, 2016; the preservation address is Wuhan University, Wuhan, China. The invention provides backup compounds fordeveloping the novel anti-tumor medicine, and has the important significance on the development and utilization of medical insect symbiotic bacterium resources.

Disclosed are pigeon pea endophytic fungi high in the yield of flavipin and an application of the pigeon pea endophytic fungi to prevent and treat plant diseases. The endophytic fungi are characterized by being chaetomium globosum (ELP-8), having been preserved at the China Center for Type Culture Collection, and having the preservation number being CCTCC No: M 2015001. The preservation address is Wuhan University at Wuhan city, and the preservation date is Jan. 5, 2015. Flavipin produced in a fermentation liquid of the pigeon pea endophytic fungi ELP-8 has an excellent inhibition effect on fusarium pathogenic fungus. The endophytic fungi have a biological prevention and treatment effect on plant diseases, caused by fusarium, of crops, vegetables, and forests, and provide a novel path and method for researching microbial pesticides.

A method for inhibiting the growth of a microorganism using an effective amount of one or more of the following synthetic brominated furanones: (i) 4-bromo-5Z-(bromomethylene)-3-methylfuran-2-one; (ii) 3-(dibromomethyl)-5-(dibromomethylene)furan-2-one; (iii) 3-(bromomethyl)-5-(dibromomethylene)furan-2-one; (iv) 4-bromo-3-(bromomethyl)-5Z-(bromomethylene)furan-2-one; or (v) 4-bromo-5-(dibromomethyl)-3-methylfuran-2(5H)-one. The brominated furanones inhibit the growth of both fungi and bacteria, including the fungal species Candida albicans, Gloeophyllum trabeum, Chaetomium globosum, and Trametes versicolor and the bacterial species Pseudomonas aeruginosa. The brominated furanones can be used topically or internally to treat human infections, and can be used to treat other objects, such as wood building supplies, to prevent fungal rot.

The invention discloses fermentation preparation and application of Chaetomium globosum dextranase and belongs to the technical fields of fermentation technology, enzymic preparations and sugar engineering. A high-yield Chaetomium globosum dextranase fermentation method is determined by optimizing medium components and fermentation conditions through orthogonal test, thereby improving the yield ofChaetomium globosum dextranase up to 698.22 U / mL; when applied to hydrolyzing high-molecular-weight glucan and at a final concentration of 2 U / ml, the Chaetomium globosum dextranase can achieve a high-molecular-weight glucan (T2000) hydrolyzing rate up to 97.9% within 15 minutes; the rate of inhibition of the Chaetomium globosum dextranase on biological membranes formed by streptococcus mutans can reach 97.9%, and the clearance rate of the biological membranes reaches up to 49.07%. The prepared Chaetomium globosum dextranase has a good application prospect in food and sugar industries and thefield of medicine.

The invention discloses an endophytic fungi chaetomium globosum strain, a microbial agent and an application thereof. The strain is named as chaetomium globosum strain ND 35, the preservation organization is the China General Microbiological Culture Collection Center, the preservation date is 9th,10,2010, the preservation number is CGMCC No. 4136. The strain is a biological beneficial bacteria with a plurality of biological control effect mechanisms, and has the plant pathogen inhibition capability with broad-spectrum and high-efficiency, and also has capability for promoting a plurality of plants growth, and is suitable for industrial production, the shelf life is long which is suitable for preventing and treating the plant diseases, so that the strain is used for producing the biological bactericide products for preventing and treating a plurality of plant diseases and multifunctional biofertilizer products for promoting the growth of plants.

The invention provides a preparation method and application of a biological seedling raising substrate of chaetomium globosum, and belongs to the technical field of agricultural microorganisms. The method comprises the steps that after preliminary fermentation is performed on agricultural waste, the agricultural waste is inoculated with chaetomium globosum ND35 sexual conidial powder to perform secondary fermentation, so that a functional flora taking chaetomium globosum as the main microorganisms in the substrate, and the biological seedling raising substrate of chaetomium globosum is obtained. The agricultural waste comprises, but is not limited to, bark, wood chips, straw and the like. The preparation method and application of the biological seedling raising substrate of chaetomium globosum have the advantages of being simple, being capable of being directly applied to the seedling raising stages of tomatoes, cucumbers and other plants, planting chaetomium globosum ND35 bacterial strains into the plants more directly and quickly, and being beneficial to the plants perpetually; the biological seedling raising substrate of chaetomium globosum is an excellent seedling raising substrate, can be applied to industrialized seedling raising of various plants, fills in the blank of domestic excellent substrates, replaces imported substrates, promotes the healthy development of industrialized seedling raising of vegetables, and has great economic and social benefits.

The invention provides preparation and application of chaetomium globosum and metabolite flavipin thereof. The strain is endophytic fungi obtained by separating gingko leaves, is identified to be chaetomium globosum with a number of CDW7, and is preserved in the China General Microbiological Culture Collection Center with the register number of CGMCC No. 6658. The invention relates to preparation and application of CDW7 metabolite flavipin at the same time. The preparation method comprises the step that the organic solvent extract of the strain CDW7 fermentation broth is subjected to gel column chromatography and recrystallization. The flavipin has strong anti-oxidation activity, and can be used as an antioxidant for application.

The invention provides a microbial inoculant used for treatment of municipal sewage. The microbial inoculant is composed of bacterial powder A, bacterial powder B, bacterial powder C and auxiliary materials, wherein the bacterial powder A contains lactic acid bacteria, bacillus subtilis, blue-green algae, saccharomycetes and trichoderma; the bacterial powder B contains Nocard's bacillus, streptomycetes, thermophilic actinomycetes, aspergillus niger and chaetomium globosum; the bacterial powder C contains aspergillus, bacillus cereus, rhizobium, bacillus flexus and pseudomonas; the auxiliary material A is composed of dipotassium phosphate, potassium dihydrogen phosphate and sodium chloride; the auxiliary material B is composed of polyaluminium chloride, sodium lignin sulfonate and sodium laurate. The inoculant is used for treatment of municipal sewage, the microbes are reasonably mixed, the municipal sewage can be treated to form water conforming to the primary emission standard within three days, and no environmental pollution is caused during the treatment process.

The invention provides a compound, a preparation method and an application thereof and a product containing the compound and relates to the technical field of compounds. The novel compound is of a structure shown as formula (I), and experiments prove that the compound can effectively regulate stress resistance of plants and improve adversity stress resistant capacity of the plants. Therefore, the product containing the compound can also have the function of regulating stress resistance of the plants. The invention further provides the preparation method of the compound. The compound is prepared from fermentation products of chaetomium globosum or chaetomium globosum spores through separation and purification. The operation is simple, the practicability is high, the blank of shortage of related research in regulation of stress resistance of the plants by metabolites in soil microorganisms is filled up, and one novel method and thought are provided for utilization of the soil microorganisms and particularly improvement of the stress resistant capacity of the plants in future.

The invention discloses a chaetomium globosum LJ-S2L1 strain and an application thereof. The preservation number of the strain is CCTCCM 2016649. The strain is simple in culture requirement, high in yield and good in antibacterial effect, growth conditions are easily controlled, and capsicum fusarium wilt pathogenic bacterium hyphae can excessively shrink, bend and partially expand through the strain. Toxicity measurement results show that the ratio of fermentation liquor of the strainS2L1 for inhibiting capsicum fusarium wilt pathogenic bacteria can reach 72.10% in 1000 micrograms / mL, median inhibiting concentration is 300.63 micro-liters / mL, and pot experiment control effect can reach 71.8% and is equal to that of 500 times dilution of 80% of carbendazim. The strain has wide application prospect in the prevention aspect of the capsicum fusarium wilt, both the fermentation liquor and sterile fermentation filtering liquid of the strain have high inhibiting effect, and the strain can serve as biological agents for the capsicum fusarium wilt.

The invention provides an application of chaetomium globosum in prevention and treatment of wheat take-all, the preservation name of the chaetomium globosum is 12XP1-2-3, the preservation number is CGMCC No.17183, the preservation unit is China General Microbiological Culture Collection Center, and the preservation date is January 23, 2019. The inhibition rate of the chaetomium globosum 12XP123 onwheat take-all pathogens GaNS90 on a flat plate is 45.4%, and field test results show that when the chaetomium globosum 12XP1-2-3 is used for wheat seed coating treatment, the white spike rate and the disease index of wheat can be obviously reduced.

The present invention provides for heterologous expression of beta-glucosidase (BGL) polypeptides encoded by Humicola grisea, Candida wickerhamii, Aspergillus aculeatus, Aspergillus oryzae, Penicillium decumbens, Chaetomium globosum, Neocallimastix frontalis, Debaryomyces hansenii, Kluyveromyces marxianus, or Phytophthora infestans in host cells, such as the yeast Saccharomyces cerevisiae. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed BGL. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.