166 results about "Trichoderma" patented technology

The invention relates to the technical field of microorganisms, in particular to application of bacillus glucans and a complex microbial inoculant thereof in preparation of bio-organic fertilizers, the strain Mh85 is separated from the root system of apple rootstock Malus hupehensis treated by a research group through trichoderma TB2, has good IAA production, nitrogen fixation, inorganic phosphorus decomposition and siderophore production capacities, and can be used for preparing bio-organic fertilizers. The strain Mh85 not only can directly promote plant growth, but also has a good growth-promoting effect on trichoderma TB2, and when the strain is compounded with the trichoderma TB2 to form a complex microbial inoculant, tomato seedling pot experiments are carried out. The complex microbial inoculant shows a synergistic effect, effectively overcomes the problems of unstable growth promoting effect, insufficient synergistic effect among strains and the like of an existing single microbial preparation, provides an efficient and stable technical scheme for improving plant growth, improving root development and increasing crop yield, and is a good raw material for preparing a bio-organic fertilizer.

The invention provides a spiropolyspora roseus strain for preventing and treating root knot nematode disease, and the preservation number of the spiropolyspora roseus strain is CGMCC No.41822. The invention also provides a microbial inoculum containing the spiropolyspora pink strain, and the microbial inoculum is prepared by the following steps: inoculating a culture medium with the spiropolyspora pink HLF-14-11, and culturing until spores are produced; spores are eluted to prepare a spore suspension, or a bacterial block is cut to serve as a liquid fermentation inoculant; inoculating the inoculant into a liquid culture medium, and carrying out shake culture; and taking the fermentation liquor to prepare liquid, powder and granules. The spiropolyspora pink strain provided by the invention is separated from root-knot nematode oocysts of diseased tomatoes, can parasitize eggs of root-knot nematodes and inhibit incubation of the eggs, fermentation filtrate of the spiropolyspora pink strain has a relatively strong poisoning effect on second-stage larvae of the root-knot nematodes, and the fatality rate of the second-stage larvae reaches 89.1%. Meanwhile, the strain can produce auxin substances such as indoleacetic acid and the like, and plant growth and root development are promoted.

The invention discloses a saline-alkali soil improvement and disease resistance double-effect controlled release fertilizer and a preparation method thereof. The fertilizer comprises 18-22% of biochar, 12-18% of furfural residues, 8-12% of cow dung compost and 6-10% of potassium humate. 5-7% of aluminum sulfate, 4-6% of sulfur and 3-5% of zeolite; the microbial inoculum comprises the following components in percentage by weight: 1.5 to 2.5 percent of saline-alkaline bacillus, 0.8 to 1.2 percent of bacillus subtilis and 0.8 to 1.2 percent 8-12% of a sodium polyacrylate coating, 4-6% of a sulfur coating and 1-3% of polyglutamic acid; 0.8 to 1.2 percent of humic acid chelated iron, 0.8 to 1.2 percent of algal polysaccharide and 0.8 to 1.2 percent of urease inhibitor; 0.8 to 1.2 percent of zinc sulfate, 0.5 to 1.0 percent of manganese sulfate and 0.5 to 1.0 percent of borax. The fertilizer can reduce the pH and conductivity of soil, increase the organic matter content of the soil and effectively improve the physicochemical properties of the soil, meanwhile, by adding the microbial agent, the disease resistance of crops can be effectively improved, the morbidity and disease index of the crops can be reduced, and nutrient release of the fertilizer has a sustained and controlled release characteristic; the initial release rate and the cumulative release rate of nitrogen are both lower than those of common chemical fertilizers, and the cumulative release rate of nitrogen is moderate within 60 days, so that the nutrient requirements of crops in different growth stages can be met.

The invention discloses a chelerythrine-containing compound microbial agent and application thereof, and belongs to the technical field of biological control. The invention provides a chelerythrine-containing compound microbial agent to solve the technical problems that in the prior art, only a single microbial agent and a plant extract are used, the biological control effect is poor, the stability is low, and the target spot is single. The compound microbial agent is prepared from a mortierella alpina extract, a trichoderma hairy extract and chelerythrine according to a specific compounding ratio. The compound microbial agent provided by the invention can delay the development of drug resistance of pathogenic bacteria through a multi-target effect, has better stability and stronger control effect, reduces the use amount of chemical pesticides, reduces the harm to the environment and human body, can be applied to tobacco planting production, can effectively reduce the control cost of tobacco black shank, and has broad application prospects. The economic benefit of tobacco planting is improved.

The present invention discloses a strain of Trichoderma wumengense GUWM22 and its application, which belong to the field of microorganisms. The deposit number of the Trichoderma wumengense GUWM22 is CCTCC NO: M20242639, the deposit date is November 26, 2024, the deposit unit is the China Center for Type Culture Collection, and the deposit address is Wuhan University, Wuhan, China. It has been verified that the GUWM22 bacteria have a strong inhibitory effect on 6 pathogens (Phytophthora nicotianae, Phytophthora capsici, Botrytis cinerea, Alternaria alternata, Phomopsis simiana and Echinops planifolia), and the inhibition rate is more than 78%. In addition, GUWM22 also has a good prevention and control effect on post-harvest anthracnose of pepper (strip pepper), with a prevention effect of 82.11%. It can be seen that Trichoderma wumengense GUWM22 is a new species of biocontrol Trichoderma with good potential. The present invention provides a new strain resource and an environmentally friendly and efficient biocontrol approach for the effective prevention and control of pepper anthracnose.

The invention provides a trichoderma maple fungicide, a microbial granular fertilizer as well as a preparation method and application of the trichoderma maple fungicide and the microbial granular fertilizer, and relates to the field of microorganisms. The acer rubrum fungicide contains acer rubrum RA with the preservation number of CGMCC No.35889. The acer rubrum fungicide has the capability of decomposing inorganic phosphorus, is wide in phosphorus dissolving range, can decompose tricalcium phosphate, ferric phosphate and aluminum phosphate, and particularly still has the very strong capability of decomposing the inorganic phosphorus under the low-temperature condition; the strain has the capability of producing siderophore and IAA at low temperature; the strain has an inhibition effect on various pathogenic bacteria causing field crop diseases. The microbial granular fertilizer provided by the invention contains various biostimulant substances, can promote crop growth, improve plant stress resistance and improve plant resistance to plant diseases and insect pests, and field tests prove that the microbial granular fertilizer can significantly promote crop growth and improve crop yield.

The invention relates to the technical field of agricultural planting, in particular to a method for preventing and treating watermelon fusarium wilt by using a green bactericide, which comprises the following steps: preparing the green bactericide which comprises the following components in percentage by mass: 18-25% of composite plant source active component consisting of water lettuce extract, enteromorpha polysaccharide and field horsetail flavone; 20%-30% of an antagonistic bacterium compound preparation, wherein the antagonistic bacterium compound preparation comprises bacillus pumilus and trichoderma; 38%-45% of a nano-carrier, wherein a chitosan-sodium alginate compound is used as an embedding material; and the balance of auxiliary components. Applying the green bactericide to watermelon plants; through component optimization and a synergistic effect, compared with the prior art, the composition has the remarkable advantages that the safety is high, all the components are from natural plants or microorganisms, no chemical pesticide residue exists, the composition is healthy to human bodies and environmentally friendly, and the problems of drug resistance and soil pollution caused by chemical agents can be avoided.

The invention provides a soil conditioner for improving soil hardening and a preparation method thereof, and relates to the technical field of soil conditioning. The soil conditioner is prepared by combining nano silicon dioxide, a straw retting material, a straw composite fermentation material, biochar, a water-retaining agent and modified starch. The straw composite fermented material is prepared by the following steps: crushing straws, mixing with sweet water, stirring, drying, carrying out microwave treatment, mixing with bacillus thuringiensis, fermenting, sterilizing, subsequently adding a composite microbial agent of bacillus subtilis, trichoderma and bacillus mucilaginosus, continuously fermenting, and drying. The modified starch is prepared by semi-gelatinizing corn starch and then carrying out microwave drying and shaping. According to the present invention, the defects in the prior art are overcome, the soil hardening phenomenon can be rapidly improved with the small addition amount, the soil porosity is increased, the activity and the diversity of the soil microorganisms are ensured, and the soil fertility is improved.

The invention relates to the technical field of agricultural biology, and discloses a microbial agent for preventing and treating fructus aurantii scab and a preparation method of the microbial agent. The microbial agent comprises the following raw materials in percentage by weight: 10-13% of paenibacillus mucilaginosus; 15%-20% of trichoderma sp. 7%-10% of paecilomyces lilacinus; 5%-8% of bacillus pumilus; 7%-9% of mycorrhizal fungi; 5%-8% of streptomyces; 5%-9% of a coptis chinensis extract; 4%-7% of a sophora flavescens extract; 2%-4% of realgar; 1%-3% of a rheum officinale extract; 10%-13% of soybean meal; 5%-8% of a water-soluble fertilizer; 7%-9% of potassium fulvate; 1%-3% of chitosan; 0.5%-1% of trace elements; 3%-8% of humic acid; the raw materials are subjected to microbial flora amplification culture, plant antibacterial component light-shielding mixing and low-temperature mixing to effectively protect the microbial activity of the microbial agent, and step-by-step operation can avoid damage of high temperature and mechanical shearing to microorganisms, so that the microbial activity of the microbial agent is effectively protected. And the loss of microbial active ingredients is reduced by vacuum drying and low-temperature granulation processes.

The invention relates to the technical field of biology, in particular to a method and application of fungus-enzyme synergistic fermentation of bagasse pith, and the method comprises the following steps: (1) adjusting the water content of the bagasse pith to 60-70%, and adjusting the pH to 4.0; (2) adding the following components according to dry matter mass: 3% of phanerochaete chrysosporium liquid with the concentration of 1.5 * 10 < 8 > CFU / ml, and uniformly mixing; 1% of sucrose; the cellulase liquid is 20000U / kg, and the cellulase liquid is prepared by dissolving crude enzyme obtained by centrifuging after trichoderma hedgehog is fermented to produce enzyme in a PBS (phosphate buffer solution); and (3) putting the mixture into a fermentation tank, and performing sealed low-oxygen fermentation for 30 days to obtain the bagasse pith feed. According to the bagasse pith feed obtained by the method, the nutritional value of the bagasse pith can be improved, the fermentation quality is improved, the digestion potential is improved, the storage time is prolonged, and high-quality feed application of the bagasse is realized.

The present invention belongs to the field of Trichoderma secondary metabolites, and discloses a natural product derived from Trichoderma harzianum, a preparation method and an application thereof. The natural product has a structure shown in Formula I. The present invention separates and purifies the Trichoderma harzianum secondary metabolites to obtain the Trichoderma harzianum source natural product LU-11, which can be used to inhibit the growth of Fusarium graminearum PH-1, a wheat head blight disease, and induce and improve the disease resistance of wheat, providing a certain theoretical basis for the development of biological pesticides.

The present invention belongs to the field of microbial technology and specifically relates to a salt-tolerant growth-promoting composite bacterial agent and its application. The active ingredients of the salt-tolerant growth-promoting composite bacterial agent are actinomycete S36, deposited with CGMCC No. 32928, Trichoderma M2, deposited with CGMCC No. 41651, and Bacillus velezensis Y10, deposited with CGMCC No. 32927. The present invention screened and obtained a composite bacterial agent obtained by mixing these three bacteria, which can improve the resistance of cotton and wheat to salt stress, providing a new microbial resource for improving the salt tolerance of cotton and wheat.

The invention discloses a complex microbial inoculant containing streptomyces elongisporus and trichoderma Guizhou, the streptomyces elongisporus is streptomyces elongisporus A25, and the trichoderma Guizhou is trichoderma Guizhou NJAU4742. The invention further discloses application of the complex microbial inoculant to promotion of cucumber growth and application to promotion of seedless litchi yield improvement and fruit quality improvement. Streptomyces elongisporus A25 and trichoderma Guizhou NJAU4742 which are separated and screened from seedless litchi tree rhizosphere soil are prepared into the composite microbial agent, the composite microbial agent is applied to a cucumber pot experiment, cucumber growth can be remarkably promoted, and when the composite microbial agent is applied to a seedless litchi field experiment, seedless litchi yield improvement and fruit quality improvement can be remarkably promoted. The complex microbial inoculant disclosed by the invention has important significance on sustainable development of the seedless litchi industry.

The invention provides compost and a preparation method thereof, and relates to the technical field of organic fertilizers, the compost comprises the following components by weight: 900-1100 parts of crop straw, 200-300 parts of animal excreta, 100-200 parts of an organic auxiliary material, and 1.5-7.5 parts of a complex microbial inoculant; the complex microbial inoculants comprise lactic acid bacteria, bacillus licheniformis, thermophilic trichoderma, actinomycetes and white rot fungi. The preparation method of the compost mainly comprises the following steps: stacking the crop straws and the organic auxiliary materials layer by layer, and compacting; uniformly sprinkling animal excrement on each layer, and then inoculating a complex microbial inoculant; punching a hole in the top of the pile to monitor the moisture content in the pile in the composting process; pile turning treatment is carried out in the composting process. According to the invention, the complex microbial inoculants are adopted for compost maturing treatment, so that the maturing speed can be increased, the maturing effect can be improved, and the content of nutrient substances in compost is increased.

The invention discloses a preparation method of a tomato culture medium, which aims at solving the technical problems that peat resources are non-renewable and high in cost and the utilization rate of mulberry twig wastes is low, and is realized by the following technical scheme: firstly, crushing fresh mulberry twigs into particles; mixing silkworm excrement with a sodium alginate solution, and coating the mulberry twig particles in a fluidized bed at the air temperature of 60 DEG C and the atomization pressure of 0.3 MPa to form core-shell structure particles with the shell thickness of 0.1-0.3 mm; then mixing the core-shell particles with fine peat to form a mixture, and stacking the mixture with the coarse peat and the fine peat to form a three-layer gradient structure; and finally, spraying a zymophyte liquid with trichoderma content greater than or equal to 1.0 * 10 < 7 > CFU / mL until the water content is 40%, and standing and activating for 20 days. The matrix takes mulberry twigs as a core raw material, combines silkworm excrement slow-release fertilizer efficiency and gradient structure optimization water-air balance, can replace a traditional peat matrix to be used for tomato planting, and realizes agricultural waste recycling and low cultivation cost.

The invention belongs to the field of fungus secondary metabolites and application thereof, and particularly relates to trichoderma reesei HK1-12 as well as a secondary metabolite and application thereof, the collection unit is China General Microbiological Culture Collection Center (CGMCC), and the collection number is CGMCC No. 41951. The trichoderma HK1-12 has excellent characteristics in the aspects of growth promotion, plant protection and the like, and is expected to replace the existing similar products to be popularized and applied.

The invention relates to the technical field of animal feed, and particularly discloses a bamboo powder dietary fiber probiotic feed additive as well as a preparation method and application thereof. The additive consists of bamboo powder dietary fibers, a probiotic agent and a carrier, wherein probiotics are at least one of trichoderma, bacillus, lactic acid bacteria and saccharomycetes. The preparation method comprises the steps of bamboo powder pretreatment, solid state fermentation, drying, mixing and the like. The product fully utilizes the prebiotics characteristics of the bamboo powder and the regulation effect of probiotics, can effectively improve the intestinal health of livestock and poultry, improve the feed utilization rate and reduce the breeding cost, can partially or completely replace antibiotics, and has the characteristics of greenness, safety and high efficiency.

The invention discloses a treatment method of fermentation wastewater. The treatment method of the fermentation wastewater comprises the following steps: adding filamentous fungi and green algae into fermentation wastewater generated after fermentation treatment of kitchen garbage, carrying out fermentation culture, then carrying out solid-liquid separation, and extracting grease from the separated solid. The filamentous fungi comprise trichoderma harzianum, and the green algae comprise one or a combination of more selected from chlorella vulgaris, chlamydomonas, haematococcus pluvialis, hylothrix, scenedesmus obliquus and chaetoclada. According to the treatment method of the fermentation wastewater, grease can be produced while the fermentation wastewater is treated, and the treatment cost of the fermentation wastewater is reduced.

The application discloses a kind of trichoderma fermentation processing indigo blue method, the product obtained and application.The method includes: preparation trichoderma seed liquid;After primary indigo blue sterilization, access seed liquid, fermentation 5-9 days under 25-30 DEG C, 120-180 rpm, avoid light, obtain secondary processing indigo blue.The application first utilizes trichoderma to carry out biological transformation to indigo blue, can significantly improve the content of its key anti-tumor component indigo carmine with specificity, and the increase is more than 150%.In vitro anti-tumor experiment shows that the inhibitory activity of secondary processing indigo blue prepared by the application to human leukemia K562 cells and human colon adenocarcinoma cell SW480 cell is significantly better than ordinary indigo blue.The product of the application can be used for preparing medicine for preventing and / or treating leukemia, colon adenocarcinoma, process controllable, suitable for industrialized production, and provides a new way for developing efficient indigo blue anticancer new drug.

This invention belongs to the field of biological agents and plant breeding technology, specifically relating to the application of *Trichoderma tumefaciens* T-51 in promoting flowering, fruit setting, and vegetative growth in plants. The *Trichoderma tumefaciens* T-51 described in this invention has the preservation number CCTCCNO: M2015729. Applying a suspension containing *Trichoderma tumefaciens* T-51 to the roots of plant seedlings can advance the flowering time and increase the number of flowers; it can also improve the number and rate of fruit set; and it can promote vegetative growth, especially increasing plant height, number of stem nodes, and number of leaves, thereby accelerating the plant breeding process and shortening the breeding cycle. This invention has very high application value in plant breeding.

This invention relates to the field of soil remediation technology, specifically disclosing a method for remediating heavy metal-contaminated soil using microbial agents. The invention uses hydroxyapatite as a carrier, utilizing its adsorption properties to load licorice extract onto its surface. To further improve the stability of the licorice extract, a carboxymethyl cellulose (CMC) solution is added. After stirring, mixing, and drying, the excellent film-forming properties of CMC help improve the stability of the licorice extract, while also increasing the specific surface area and adsorption sites of the hydroxyapatite. The introduction of licorice extract and CMC not only enhances the chelating ability of hydroxyapatite for heavy metals but also provides an additional carbon source for microorganisms, promoting their growth and reproduction. The metabolic activity of *Trichoderma solani* and *Bacillus licheniformis* is significantly enhanced, resulting in a substantial increase in remediation efficiency.

This invention discloses a method for processing bamboo shoot residue that synergistically enhances the content of soluble dietary fiber and its bound polyphenols. Using waste bamboo shoot residue as raw material, the fermentation bacteria are all food-grade. Specifically, a multi-strain coupled fermentation is employed, including *Bacillus belye*, *Bacillus natto*, *Trichoderma kangsei*, *Cladosporium*, *Aspergillus pamoateus*, and *Bacillus coagulans*. This significantly alters the ratio and content of soluble and insoluble dietary fiber in the bamboo shoot residue and substantially increases the content of dietary fiber-bound polyphenols, synergistically enhancing the content of soluble dietary fiber and its bound polyphenols, thereby producing high-quality dietary fiber. This invention is simple to operate, low in cost, and allows for easier control of fermentation conditions. It is highly efficient and does not easily damage the non-covalent bonds (hydrogen bonds, electrostatic or ionic bonds, etc.) or covalent bonds (ester bonds, ether bonds, etc.) binding dietary fiber and polyphenols. It also has no significant impact on the pH and temperature of the environment, and can significantly increase the content of soluble dietary fiber and its bound polyphenols in bamboo shoot residue.

This invention belongs to the field of bio-mineral fertilizer technology, specifically relating to a microbial liquid compound agent and bio-mineral fertilizer that combine coal gangue activation and heavy metal remediation. The microbial liquid compound agent comprises the following functional strains prepared through three-stage sequential inoculation and fermentation using electrochemical low-Hertz water: *Pseudomonas putida* (a remedial bacterium), *Azotobacter chrysogenum* (a nitrogen-fixing bacterium), *Azospirillum brasiliensis* (a growth-promoting bacterium), *Pseudomonas fluorescens* (a phosphate-solubilizing bacterium), *Bacillus subtilis* (a biocontrol bacterium), *Bacillus mucilaginosus* (a potassium-solubilizing bacterium), *Bacillus megaterium* (a silica-solubilizing bacterium), and *Trichoderma echinococcus* (a synergistic bacterium). The bio-mineral fertilizer is prepared using the aforementioned microbial liquid compound agent, with coal gangue powder as the main raw material. The scientific combination of different strains provided by this invention achieves efficient improvement of heavy metal-contaminated land and increased crop yields. Simultaneously, it transforms coal gangue into a valuable resource, solving the pollution problem caused by the large-scale accumulation of coal gangue, and possesses significant economic and social value.

The invention belongs to the technical field of genetic engineering and biology, and relates to application of trichoderma harzianum TrVPT1 in regulation and control of trichoderma phosphorus accumulation and gibberellic disease resistance. The nucleotide sequence of the trichoderma harzianum vacuole phosphate transporter gene TrVPT1 is as shown in SEQ ID NO: 1. The invention proves that the TrVPT1 gene has an important regulation and control effect in trichoderma phosphorus steady-state regulation and control and biological prevention and control functions.

The invention discloses a LeRho1 gene expression vector and a method for improving heat resistance and trichoderma resistance of lentinus edodes. The LeRho1 gene expression vector is a recombinant expression vector, a target gene for coding the LeRho1 protein is inserted into the recombinant expression vector, and the amino acid sequence of the LeRho1 protein is shown as SEQ ID NO. 1. The invention finds that the LeRho1 gene has a positive regulation effect on the heat resistance of the shiitake mushroom strain, and the LeUSPA and LeUSPH genes mediate the thermal response of the shiitake mushroom strain through negative regulation. Therefore, according to the method, the LeRho1 gene overexpression vector or LeUSPA and LeUSPH gene silencing vectors are constructed and transferred into the shiitake mushroom strain for genetic transformation, the heat resistance and trichoderma resistance of the shiitake mushroom strain can be remarkably improved, and the transformation efficiency is high.

The present invention discloses a method for preparing β-glucose oligosaccharides, and relates to the field of fermentation technology. The method comprises the following steps: (1) preparing a Bacillus faecalis seed solution; (2) preparing a Trichoderma LE02 seed solution; (3) fermenting: inoculating the Bacillus faecalis seed solution into a fermentation medium, and inoculating the Trichoderma LE02 seed solution once at 8-12 hours, 45-55 hours, and 85-95 hours after the inoculation of the Bacillus faecalis seed solution, respectively. The method is ventilated and fermented for 120 hours to obtain a β-1,3-glucose oligosaccharide fermentation liquid; and (4) purifying. The present invention, by extending the fermentation time or adding the Trichoderma LE02 seed solution in batches, ultimately obtains a thermogelling oligosaccharide β-1,3-glucose oligosaccharide with a lower degree of polymerization and a higher concentration, with a yield of up to 89-93%, which can be industrialized.

The invention discloses chitinase activity and application of a trehalose-6-phosphate synthase homologous protein. The trehalose-6-phosphate synthase is selected from any one of the following proteins: A1) a protein as shown in any one of SEQ ID NO.1-8; a2) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid as shown in any one of SEQ ID NO.1-8 and has chitin synthase activity; a3) a protein derived from Trichoderma, yeast, Aspergillus nidulans, Aspergillus fumigatus, Candida albicans, Magnaporthe oryzae, Alternaria alternata and Escherichia coli, having 98% or more of identity with the protein of A1) and having chitin synthase activity; and A4) a fusion protein obtained by connecting a label to the N end or C end of the sequence A1) or A2) or A3). The Tpss disclosed by the invention has chitin synthase activity, can be used for synthesizing chitin from UDP-GlcNAc, and has a very good application prospect.

The invention relates to the technical field of vegetable disease and pest control, and discloses a green vegetable disease and pest control method based on a microbial preparation, which comprises the following steps: selecting an application mode according to a target disease and pest type and a vegetable growth stage; a microbial preparation working solution or a mixture is prepared, the microbial preparation contains beneficial microorganisms, and the beneficial microorganisms comprise one or more of bacillus subtilis, trichoderma, paecilomyces lilacinus, metarhizium anisopliae or beauveria bassiana. According to the green prevention and control method for vegetable diseases and insect pests based on the microbial preparation, the adopted microbial preparation mainly comprises beneficial microorganisms such as bacillus subtilis, trichoderma and paecilomyces lilacinus and natural metabolites of the beneficial microorganisms and does not contain any chemical synthetic pesticide, and no toxic and harmful substance residues exist after the method is used; the pollution of traditional chemical pesticides to soil, water sources and air is fundamentally avoided, the farmland ecological environment is effectively protected, and the pesticide is safe to non-target organisms such as pollination insects and soil beneficial organisms.

The invention discloses a preparation method of a co-culture metabolite carbon-based biostimulant based on different combinations of trichoderma, bacillus and metarhizium anisopliae. Compared with a single microorganism culture metabolite carbon-based preparation, the metabolite carbon-based preparation co-cultured by different combined micro-substances has obvious synergism in the aspects of wheat growth promotion and stress resistance improvement; the optimal proportion and time of the metabolite co-cultured by different combinations of the trichoderma, the bacillus velezensis and the metarhizium anisopliae adsorbed by the rice straw biochar are obvious in synergistic interaction in promoting wheat seed germination and seedling growth and improving wheat stress resistance. Different combinations of co-culture metabolites and biochar compounded preparations can be developed into novel carbon-based biostimulant, and the carbon-based biostimulant has important significance in promoting growth of wheat and corn, improving stress resistance and disease resistance and the like.