1019 results about "Trichoderma" patented technology

A composite biological agent with ternary microorganism for composting process of agricultural waste is composed of a composite biological agent I, a composite biological agent II, and a composite biological agent III, which is characterized in that the composite biological agent I is composed of bacillus, actinomycetes, microzyme and lactobacillus, the composite biological agent II is composed of trichoderma, ergillus, penicillium and trametes versicolor, and the composite biological agent III is composed of photosynthetic bacterium, azotobacter, phosphorus bacteria, and silicate bacterium. 30-60% composite biological agent I, 30-60% composite biological agent II, 10-20% composite biological agent III are mixed or applied to the process of composting process of agricultural waste in phases. Each component ratio and recruitment of biological agents can be both properly adjusted with different dung materials and dung purpose, thereby achieving purpose of using microorganism resources efficiently and raising fermentation efficiency.

PCT No. PCT/IL98/00437 Sec. 371 Date Feb. 8, 2000 Sec. 102(e) Date Feb. 8, 2000 PCT Filed Sep. 10, 1998 PCT Pub. No. WO99/12429 PCT Pub. Date Mar. 18, 1999A method of bioconversion of organic industrial or agricultural cellulose containing wastes into proteinaceous product. The method comprises comminution of the wastes with moistening and addition of a starting culture inducing their biological degradation and conversion into simple carbohydrates. The carbohydrates are fermented into digestible products. The starting culture comprises cleaving enzymes produced by edible microorganisms such as fungus and bacteria selected from the group consisting of Ilumicola grisea, Trichoderma harzanum, Ruminococcus albus.

The invention relates to a biotechnology dealing agricultural discard stem with an highly effective stem decomposing composite bacterial, which dissolves the problem of single species of applying microbiological bacterial, weak breaking up, low total degradability rate of cellulose, long developmental cycle. The composite bacterial comprises the following parts: yellow archespore wool bacterium leather bacterial, mushroom bacterial, haci trichoderma, green trichoderma, coninic trichoderma, aspergillus niger, bacillus subtilis, feed bacillus, bacillus megaterium, candida, distillery yeast, food-yeast, brown sphere azotobacter, thermoactinomyces, and so on. The composite bacterial can overall degrade lignin, cellulose, hemicellulose and other organic material in the stem, has a more than 80% degrading rate, a 10-15 days short cycle, keeps a long time for providing a solid bacteria.

The invention discloses microbial ecological organic fertilizer and a preparation method thereof. The organic chemical fertilizer comprises the following components: edible mushroom dregs, sugarcane dregs, peat soil, oil squeezed waste, filter sludge, livestock and poultry manure and a microbial agent, wherein the microbial agent comprises bacillus subtilis, saccharomycetes, aspergillus niger, guanyloribonuclease, trichoderma, lactobacillus, photosynthetic bacteria, actinomycetes and streptomyces microflavus. The prepared organic fertilizer has advantages of wide raw material source, low cost, high absorption utilization rate, comprehensive nutrients, low dosage, no odor, fast fertilizer effect, various bacteria types, high content, capability of preventing injurious pests, simple preparation method and the like.

The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.

The invention relates to the field of environmental protection and discloses a soil conditioner, as well as a preparation method and application thereof. The soil conditioner comprises raw materials in parts by weight: 50 to 60 parts of wood fibers, 14 to 18 parts of biochar, 25 to 30 parts of humus, 3 to 6 parts of polysaccharide, 1 to 3 parts of seaweed essence, 0.001 to 0.005 part of Trichoderma and 0.001 to 0.005 part of nitrogen fixation bacteria. The invention also discloses a preparation method, the application and a using method of the soil conditioner, the soil conditioner has the advantages of improving soil physical and chemical properties and promoting the turf forming performance of plants, and the preparation and application methods are simple and convenient, so that the popularization and application are facilitated.

The present invention is one kind of soil improving agent for vegetable plot and its preparation process and application, and belongs to the field of soil improving microbe preparation technology. The soil improving agent consists of turf, diatomite, microbe preparation, peanut cake and fly ash, and its microbe preparation includes Bacillus subtilis fermenting liquid, actimomycetale fermenting liquid and trichoderma fermenting liquid in the weight ratio of 1 to 0.5-3.5 to 0.5-6. It is applied to soil after being mixed in the field, can improve soil, and raise crop yield and quality obviously.

The invention relates to a method for jointly treating stalks by steam explosion and microorganism fermentation, comprising the following steps of: firstly, carrying out steam explosion pretreatment on the stalks to obtain exploded stalks; then preparing spore seed liquid: inoculating aspergillus oryzae or trichoderma Koningi to a PDA (Potato Dextrose Agar) culture medium, standing and culturing for 3-5 days at the temperature of 28-32 DEG C, and preparing the spore seed liquid with the concentration of 106-108 per mL; and finally carrying out microorganism fermentation: uniformly mixing 18g of exploded stalks, 2g of bran and 30mL of mineral element nutrient solution, adjusting pH to be 7.0 with Ca(OH)2, sterilizing for 15min at the temperature of 121 DEG C and the pressure of 0.15MPa to obtain a solid fermentation medium a, inoculating the spore seed liquid according to 2-4% of inoculum size, and culturing the spore seed liquid for 5-7 days at the temperature of 28-32 DEG C. The fermentation stalks obtained by utilizing the method have low content of lignose, cellulose and hemicellulose and high activity of filter paper carbohydrase, CMC (carboxymethyl cellulose) enzyme, amylase and protease.

The invention relates to a diseases-prevention organic fertilizer prepared by edible fungi residue, comprising the following steps of: adding trichoderma fermentation liquid into decomposed edible fungi residue, and preparing trichoderma fermentation substance; adding actinomycete fermentation liquid into decomposed edible fungi residue, and preparing actinomycete fermentation substance; evenly mixing the trichoderma fermentation substance and the actinomycete fermentation substance according to the mass ratio of 0.5-1:0.5-1; and airing the obtained mixture until the moisture content is 20-22% at the normal temperature; wherein the decomposed edible fungi residue is prepared by stacking and fermenting 75-85 parts of edible fungi residue, 15-20 parts of cake and 1-2 parts of microbial fermentation fungicide, and airing until the moisture content is 25-35%. The diseases-prevention organic fertilizer has high effective component, good diseases-prevention effect, safety, environmental protection and low cost.

The invention discloses a method for hydrolyzing stevioside with rich sources by using acid and synchronously preparing two products with high added values, namely isosteviol and sophorose. An isosteviol product is a medicine with functions of reducing blood sugar, regulating lipid, and the like, the sophorose is a most effective inducer for producing cellulase from strains such as trichoderma, and the like, and the two products have broad application prospect respectively. The method is characterized in that by controlling appropriate conditions, the two high added value products with important industrial application value and medical value, namely the isosteviol and the sophorose are synchronously prepared, and a one-step hydrolysis method is adopted.

Described herein are methods to enhance the production of more highly fermentable carbohydrates in plants, especially forage grasses. The invention provides for transgenic plants transformed with expression vectors containing a DNA sequence encoding ferulic acid esterase I from Aspergillus, preferably A. niger. The expression vectors may optionally comprise a DNA sequence encoding xylanase from Trichoderma, preferably T. reesei. Expression of the enzyme(s) is targeted to specific cellular compartments, in specific tissues and under specific environmental conditions. Uses of this invention include, but are not limited to, forage with improved digestibility for livestock, and enhanced biomass conversion.

The invention discloses a trichoderma spore powder as well as a preparation method and application thereof. The trichoderma spore powder contains trichoderma spore powder and a drying aid, wherein the trichoderma spore powder is the spore powder of Trichoderma sperellum T1 which is preserved in the general microorganism center of CCCCM (China Committee for Culture Collection of Microorganisms) and has the preservation number of CGMCC NO: 3531 and a rDNA ITS region segment sequence shown in SEQ ID NO:1; the drying aid is one or more of beta-cyclodextrin, potato starch and soluble starch. The trichoderma spore powder has an effective prevention and control effect on banana vasicular wilt, and has a remarkable antagonism effect on various pathogenic funguses, especially on soil-borne funguses. The preparation time is saved with the preparation method of the trichoderma spore powder, which can be used for preparing the trichoderma spore powder which has high density, high germination rateand high drying efficiency and is easy to store, transport and use.

The invention relates to a high-efficiency hardening-resisting continuous-cropping-resisting soil-modifying biological agent. The biological agent is composed of: 20% of trichoderma, 20% of streptomyces microflavus, 30% of starch, 10% of grass peat, 8.15% of a siliceous fertilizer, 6% of humic acid, 5% of urea, 0.2% of biological strains (including 1/3 of bacillus subtilis, 1/3 of bacillus cereus, and 1/3 of bacillus licheniformis), 0.1% of vitamin C, 0.5% of compound sodium nitrophenolate, and 0.05% of DA-6. With the soil-modifying biological agent provided by the invention, defects of existing fertilizers are overcome, the pollution to the environment is reduced, soil is activated, soil hardening and continuous-cropping are overcome, and substantial influences are provided for healthy growth and bumper harvest of crops.

The invention provides the application of trichoderma reesei and sedum plumbizincicola in the remediation of cadmium-polluted farmland soil. The application comprises the following steps of: cutting sedum plumbizincicola seedlings with about 10cm of plant height into soil to be remedied, applying trichoderma reesei FS10-C fermentation liquor or microbial preparation to the rhizosphere of the plant, keeping the soil water content to be 70%wt of the maximum field capacity in the process of remediation, and scissoring the plant along the surface of soil after the plant grows for 60-150 days to complete one remediation process. According to the application, the plant remediation efficiency of the cadmium-polluted farmland soil can be improved; and the sedum plumbizincicola plant remediation efficiency can be improved according to the trichoderma preparation, the biomass of the soil microorganism can be enhanced, the micro-ecological environment of the soil can be improved, and the application is good in ecological efficiency, and suitable for the remediation of the cadmium-polluted farmland soil.

The invention relates to a composite microbe fertilizer. The composite microbe fertilizer is prepared by 25-35 wt% of vegetable garden soil, 5-10 wt% of active functional bacteria, 5-15 wt% of mineral powder, 25-30 wt% of an organic fertilizer, 15-20 wt% of an inorganic composite fertilizer, and water; wherein the sum of the components is 100%. The active functional bacteria comprise lactic acid bacteria, bacillus subtilis, Bacillus megaterium, trichoderma, aspergillus niger, monascus, Trichoderma harzianum, aspergillus aculeatus and yeast, the mineral powder is a mixture of one or more of peat, zeolite, vermiculite, montmorillonite, calcium carbonate and expanded perlite, fineness of the mineral powder is less than or equal to 200 meshes, the organic fertilizer contains decomposed manure, a rapeseed cake fertilizer, and straw, and the weight ratio of the manure to the rapeseed cake fertilizer to straw is 5.5-7.5:2.5-3.5:0.5-1.5.

The invention relates to the field of banana cultivation, in particular to a compound microbial agent for preventing and treating a banana wilt disease and a prevention and treatment method. The compound microbial agent comprises paenibacillus polymyxa, bacillus subtilis, bacillus thuringiensis, pseudomonas stutzeri, bacillus amyloliquefaciens, cold-resistant brevibacteriaceae, trichoderma and metarrhizium anisopliae. By means of a reasonable proportion, all strains are mutually influenced and promoted, and the prevention and treatment effect on the banana wilt disease is greatly improved. The prevention and treatment method of the banana wilt disease can effectively adjust the composition of bacterial colonies in soil, promote growth and propagation of probiotics and antagonistic bacteria in the soil, rapidly restore and stabilize the balance of the bacterial colonies in the soil, prevent and treat occurrence and spread of the banana wilt disease, effectively decrease the occurrence rate of the banana wilt disease and maintain the stable and high yield of bananas.

The biological and biochemical preparation is a kind of multiple target site preparation containing Trichoderma pseudokiningii and Bacillus spp as well as cell membrane protecting matter, composite inductor, natural antibiotic matter, such as trace elements, and beneficial matters, such as taurine, vitamin C, etc. Trichoderma pseudokiningii is strain obtained through separating and screening stalk and named as TRIP1, and Bacillus spp is separated from soil and named as BAC1. The biological and biochemical preparation may be used in preventing and controlling several kinds of plant diseases, promoting growth of plant, protecting cell membrane and raising plant photochemical efficiency.

The invention discloses compound fertilizer having growth promotion and high slow-release properties and used for rice planting. The compound fertilizer is prepared from raw materials as follows: ammonium nitrate, monopotassium phosphate, urea, urea phosphate, calcium superphosphate, ammonium dihydrogen phosphate, potassium sulfate, magnesium sulfate, potassium nitrate, amino sodium dicarboxylate, Trichoderma, yeast powder, straw fermentation bacteria, cellulase, potassium mineral powder, rare earth, low-rank coal, silico-calcium powder, turf powder, fly ash, pine sawdust, kaolin, rice hull powder, poultry manure, maize straw, blue-green alga powder, dolomite, diatomite, calcite, humic acid, lignin, EDTA (ethylene diamine tetraacetic acid) chelate zinc, amino acid chelated molybdenum, dicyandiamide, phosphoric triamide and a modified slow-release agent. The invention further discloses a preparation method of the compound fertilizer having growth promotion and high slow-release properties and used for rice planting. The compound organic fertilizer has outstanding slow-release performance and can effectively increase the rice yield and improve the rice quality.

The invention belongs to the technical field of organic fertilizer, in particular to an antibacterial type composite biological organic liquid fertilizer, which comprises bacillus subtilis with the capacity of intensively decomposing enzyme and inhibiting bacteria, pseudomonades fluorescens are rhizosphere soil probiotics which can decompose pesticide residues and dissolve insoluble microcosmic salts of the soil, antibiotic actinomycetes secrete antibiotics so as to help to reduce the demand of chemical pesticides, trichoderma not only can prevent a variety of pathogenic bacteria, but also can decompose straw crude fiber so as to improve soil physical property and increase the content of available organic matters in the soil. The product also comprises chitin with the functions of sterilization and growth promotion. The bio-technology components are matched with organic and inorganic nutrients to synthesize a dual-effect novel biological agent with fertilizers and natural bio-fungicides. Therefore, the product is an organic green product and can be widely used for various crops.

The invention discloses a compound bacterium agent and a method for accelerating fast maturity by inoculating compound bacterium agent to compost. The method comprises the steps of: performing enlargement cultivation respectively on high-temperature microbial strains such as Geobacillus, Bacillus licheniformis, two trichoderma, actinomyces and the like which are screened from the matured compost, and combining the strains in certain proportion into the compound bacterium agent. The compound bacterium agent is inoculated to the compost in certain proportion, the compost is moved into a fermentation tank for aerated aerobic fermentation, and the compost maturity is completed. Compared with the control compost, the inoculated compost of the scheme of the invention reaches the high temperature period one week earlier than the control compost, and the high temperature period lasts for 14 days so as to be beneficial to the decomposition and humification of organic matters of the compost; and the inoculated compost is thoroughly decomposed about twenty days later. Through test, most of the weed seeds, ascarid eggs and colibacillus in the compost are dead, the humic acid content is 25 g/kg higher than that of the control compost, the humification coefficient is more than 1.9, the decomposed finished product is black brown and odourless, and has excellent stability, harmless and minimized quality, apparently environmental and economic benefits.