71 results about "Trichoderma" patented technology

The invention provides a trichoderma maple fungicide, a microbial granular fertilizer as well as a preparation method and application of the trichoderma maple fungicide and the microbial granular fertilizer, and relates to the field of microorganisms. The acer rubrum fungicide contains acer rubrum RA with the preservation number of CGMCC No.35889. The acer rubrum fungicide has the capability of decomposing inorganic phosphorus, is wide in phosphorus dissolving range, can decompose tricalcium phosphate, ferric phosphate and aluminum phosphate, and particularly still has the very strong capability of decomposing the inorganic phosphorus under the low-temperature condition; the strain has the capability of producing siderophore and IAA at low temperature; the strain has an inhibition effect on various pathogenic bacteria causing field crop diseases. The microbial granular fertilizer provided by the invention contains various biostimulant substances, can promote crop growth, improve plant stress resistance and improve plant resistance to plant diseases and insect pests, and field tests prove that the microbial granular fertilizer can significantly promote crop growth and improve crop yield.

The invention discloses a complex microbial inoculant containing streptomyces elongisporus and trichoderma Guizhou, the streptomyces elongisporus is streptomyces elongisporus A25, and the trichoderma Guizhou is trichoderma Guizhou NJAU4742. The invention further discloses application of the complex microbial inoculant to promotion of cucumber growth and application to promotion of seedless litchi yield improvement and fruit quality improvement. Streptomyces elongisporus A25 and trichoderma Guizhou NJAU4742 which are separated and screened from seedless litchi tree rhizosphere soil are prepared into the composite microbial agent, the composite microbial agent is applied to a cucumber pot experiment, cucumber growth can be remarkably promoted, and when the composite microbial agent is applied to a seedless litchi field experiment, seedless litchi yield improvement and fruit quality improvement can be remarkably promoted. The complex microbial inoculant disclosed by the invention has important significance on sustainable development of the seedless litchi industry.

The invention belongs to the field of fungus secondary metabolites and application thereof, and particularly relates to trichoderma reesei HK1-12 as well as a secondary metabolite and application thereof, the collection unit is China General Microbiological Culture Collection Center (CGMCC), and the collection number is CGMCC No. 41951. The trichoderma HK1-12 has excellent characteristics in the aspects of growth promotion, plant protection and the like, and is expected to replace the existing similar products to be popularized and applied.

The application discloses a kind of trichoderma fermentation processing indigo blue method, the product obtained and application.The method includes: preparation trichoderma seed liquid;After primary indigo blue sterilization, access seed liquid, fermentation 5-9 days under 25-30 DEG C, 120-180 rpm, avoid light, obtain secondary processing indigo blue.The application first utilizes trichoderma to carry out biological transformation to indigo blue, can significantly improve the content of its key anti-tumor component indigo carmine with specificity, and the increase is more than 150%.In vitro anti-tumor experiment shows that the inhibitory activity of secondary processing indigo blue prepared by the application to human leukemia K562 cells and human colon adenocarcinoma cell SW480 cell is significantly better than ordinary indigo blue.The product of the application can be used for preparing medicine for preventing and / or treating leukemia, colon adenocarcinoma, process controllable, suitable for industrialized production, and provides a new way for developing efficient indigo blue anticancer new drug.

This invention relates to the field of soil remediation technology, specifically disclosing a method for remediating heavy metal-contaminated soil using microbial agents. The invention uses hydroxyapatite as a carrier, utilizing its adsorption properties to load licorice extract onto its surface. To further improve the stability of the licorice extract, a carboxymethyl cellulose (CMC) solution is added. After stirring, mixing, and drying, the excellent film-forming properties of CMC help improve the stability of the licorice extract, while also increasing the specific surface area and adsorption sites of the hydroxyapatite. The introduction of licorice extract and CMC not only enhances the chelating ability of hydroxyapatite for heavy metals but also provides an additional carbon source for microorganisms, promoting their growth and reproduction. The metabolic activity of *Trichoderma solani* and *Bacillus licheniformis* is significantly enhanced, resulting in a substantial increase in remediation efficiency.

This invention belongs to the field of bio-mineral fertilizer technology, specifically relating to a microbial liquid compound agent and bio-mineral fertilizer that combine coal gangue activation and heavy metal remediation. The microbial liquid compound agent comprises the following functional strains prepared through three-stage sequential inoculation and fermentation using electrochemical low-Hertz water: *Pseudomonas putida* (a remedial bacterium), *Azotobacter chrysogenum* (a nitrogen-fixing bacterium), *Azospirillum brasiliensis* (a growth-promoting bacterium), *Pseudomonas fluorescens* (a phosphate-solubilizing bacterium), *Bacillus subtilis* (a biocontrol bacterium), *Bacillus mucilaginosus* (a potassium-solubilizing bacterium), *Bacillus megaterium* (a silica-solubilizing bacterium), and *Trichoderma echinococcus* (a synergistic bacterium). The bio-mineral fertilizer is prepared using the aforementioned microbial liquid compound agent, with coal gangue powder as the main raw material. The scientific combination of different strains provided by this invention achieves efficient improvement of heavy metal-contaminated land and increased crop yields. Simultaneously, it transforms coal gangue into a valuable resource, solving the pollution problem caused by the large-scale accumulation of coal gangue, and possesses significant economic and social value.

The invention discloses a LeRho1 gene expression vector and a method for improving heat resistance and trichoderma resistance of lentinus edodes. The LeRho1 gene expression vector is a recombinant expression vector, a target gene for coding the LeRho1 protein is inserted into the recombinant expression vector, and the amino acid sequence of the LeRho1 protein is shown as SEQ ID NO. 1. The invention finds that the LeRho1 gene has a positive regulation effect on the heat resistance of the shiitake mushroom strain, and the LeUSPA and LeUSPH genes mediate the thermal response of the shiitake mushroom strain through negative regulation. Therefore, according to the method, the LeRho1 gene overexpression vector or LeUSPA and LeUSPH gene silencing vectors are constructed and transferred into the shiitake mushroom strain for genetic transformation, the heat resistance and trichoderma resistance of the shiitake mushroom strain can be remarkably improved, and the transformation efficiency is high.

The invention discloses a preparation method of a co-culture metabolite carbon-based biostimulant based on different combinations of trichoderma, bacillus and metarhizium anisopliae. Compared with a single microorganism culture metabolite carbon-based preparation, the metabolite carbon-based preparation co-cultured by different combined micro-substances has obvious synergism in the aspects of wheat growth promotion and stress resistance improvement; the optimal proportion and time of the metabolite co-cultured by different combinations of the trichoderma, the bacillus velezensis and the metarhizium anisopliae adsorbed by the rice straw biochar are obvious in synergistic interaction in promoting wheat seed germination and seedling growth and improving wheat stress resistance. Different combinations of co-culture metabolites and biochar compounded preparations can be developed into novel carbon-based biostimulant, and the carbon-based biostimulant has important significance in promoting growth of wheat and corn, improving stress resistance and disease resistance and the like.

This invention relates to a microbial composition for preventing and controlling root rot in tea trees and its application, belonging to the field of microbial and organic fertilizer technology. The composition comprises the following components: Component 1, Bacillus kaempferi and Trichoderma yunnanensis; Component 2, humic acid, Chrysanthemum indicum extract, amino acids, brown algae oligosaccharides, complex vitamins, and trace elements; wherein, Bacillus kaempferi is N4471 (…). Bacillus cabrialesii The accession number is CCTCC NO: M20221660, and the Yunnan Trichoderma is YNK-FG0001 ( Trichoderma yunnanense The microbial composition, with accession number CCTCC NO: M 2025645, significantly improved soil water stability aggregates, pH, and cation exchange capacity when applied to tea plant cultivation. It also enhanced root vitality and the activity of antioxidant enzymes [superoxide dismutase (SOD) and peroxidase (POD)], thereby reducing the growth of Fusarium rot (…). Fusarium solani ), Pythium ( Pythium spinosum ) and teosinte ( Rosellinia arched The disease index of tea tree root rot caused by infection was used to ultimately achieve effective control of tea tree root rot.

The invention relates to the technical field of special fertilizer effect compositions, and particularly discloses a seedling raising and plant promoting composition for mountain mangoes and a rapid production and field planting method. The composition comprises various components such as microbial metabolite fermentation liquor dry powder, trichoderma fermentation filter residue powder, tea bran residue crushed matter, perlite loaded chitosan oligosaccharide composite particles, a kaolin-sepiolite compound and sodium alginate gel particles, and the core components are matched and coordinated to construct a stable seedling environment structure. The invention further provides a rapid production and field planting method matched with the composition, the whole process of seedling raising, bag transferring, shaping and management before outplanting is covered, and the method is suitable for intensive cultivation and efficient transplanting of mangoes under complex mountain conditions and has good technical adaptability and application prospects.

The present application belongs to the field of microorganisms and biological control technology, and particularly relates to Burkholderia cenocepacia Tzs39 and application thereof in preventing and treating plant pathogenic fungi. The present application provides a Burkholderia cenocepacia Tzs39, and the preservation number of the Burkholderia cenocepacia Tzs39 is CGMCC No.30082. The antagonistic bacteria Tzs39 in the present application is isolated from rhizosphere soil of continuously cultivated Radix Pseudoginseng, has a good rhizosphere colonization effect and is safe and reliable, and can be used for preventing and treating or alleviating soil-borne disease problems of continuously cultivated medicinal plants, preventing and treating root rot in the process of continuously cultivating medicinal plants. The results of the embodiments show that the antagonistic bacteria Tzs39 can effectively inhibit the growth of Fusarium oxysporum and Trichoderma.

This invention belongs to the field of biological agent technology, specifically relating to a strain of *Trichoderma*, a biocontrol agent, a bio-organic fertilizer, and methods and applications for controlling plant diseases. This invention provides a strain of *Trichoderma* P11, with accession number CCTCC NO: M 2024648. The *Trichoderma* P11 of this invention can enhance positively correlated disease resistance genes in plants. PnCAD , PnSN1 , PnDEFL1 The relative expression level of *Trichoderma* P11 was increased, enhancing the activity of disease resistance-related enzymes SOD, POD, and CAT. Furthermore, *Trichoderma* P11 could damage the cell membrane of pathogens, inhibiting their growth. *Trichoderma* P11 also exhibits good rhizosphere colonization ability. The results of the examples show that *Trichoderma* P11 of the present invention can reduce the incidence of root rot in Panax notoginseng fields and can also antagonize various pathogens such as *Pseudomonas aeruginosa*, *Pseudomonas oryzae*, *Geotrichum candida*, *Early Blight of Tomato*, *Phytophthora indicum*, *Phytophthora capsici*, and *Alternaria spiralis*.

This invention belongs to the field of agricultural technology, specifically disclosing a method for improving the utilization rate of fertilizers and mineral nutrients under different continuous cropping years. First, soil samples are taken from experimental sites with different continuous cropping years for testing. Then, the experimental sites are furrowed and ridged, and fertilization is carried out according to the adjusted fertilization plan based on conventional fertilization. After fertilization, tobacco seedlings are transplanted. Conventional field management is then carried out on the transplanted experimental fields. Samples are taken for analysis 70-80 days after transplanting to determine the utilization rate of fertilizers and mineral nutrients. This invention optimizes fertilization by reducing nitrogen and increasing potassium, increasing the application of maifanite, applying high-carbon base fertilizer, and using Trichoderma root irrigation. This can increase the accumulation of dry matter and nutrient absorption in various parts of the tobacco plant, significantly improving fertilizer and mineral nutrient utilization rates. It provides a feasible solution for overcoming the obstacles of continuous cropping in tobacco-growing areas, improving the utilization rate of fertilizers and mineral elements, reducing fertilizer application, promoting sustainable tobacco production, and improving the yield and quality of tobacco leaves.

The invention relates to a pig manure rapid fermentation-soil remediation combined type complex microbial inoculant and application thereof.The complex microbial inoculant is formed by compounding bacillus subtilis, white rot fungi and trichoderma according to a specific proportion, and the bacillus subtilis strain is derived from an efficient compost isolate separated from composted pig manure compost. The microbial inoculum is prepared by adding the composite protective agent to be mixed with the carrier and performing low-temperature drying. During application, the pig manure is subjected to continuous high-energy quantum treatment, and pathogen inactivation and organic matter degradation are achieved through the synergistic effect of a high-frequency electric field and active particles; and then carrying out solid-liquid separation, inoculating the solid part with a complex microbial inoculant, carrying out short-period aerobic fermentation at 40-80 DEG C, and carrying out low-temperature airflow drying at 80-150 DEG C to prepare the solid organic fertilizer with both fertilizer efficiency and soil biocontrol function. And the liquid part is subjected to bipolar high energy quantum treatment and chemical neutralization to produce reclaimed water. Through systematic combination of high-energy quantum activation and the functional complex microbial inoculant, rapid and efficient fermentation and all-component resource utilization of pig manure are realized.

This invention discloses a *Hymenopellis raphanipes* mutant strain EBD13 and its applications. The invention measured and observed the growth rates and vigor of the mutant strain, the original strain, and commercially available strains in the mother culture, original culture, and cultivated culture, respectively, and statistically analyzed the fruiting results. Finally, a fast-growing *Hymenopellis raphanipes* mutant strain EBD13 was selected, which can produce fruit without soil covering. This strain can significantly reduce production costs for enterprises and solves the problems of requiring large amounts of manpower and soil for *Hymenopellis raphanipes* cultivation, resulting in significant economic benefits.

The invention discloses a trichoderma fungus Y4 and application thereof in promoting rice growth, and belongs to the technical field of microorganisms. The classification name of the trichoderma fungus Y4 is Trichoderma sp.Y4, the trichoderma fungus Y4 is preserved in the China Center for Type Culture Collection, and the preservation number of the trichoderma fungus Y4 is CCTCC NO: M 2026215. And biological characteristics and molecular identification are combined to identify that the trichoderma fungus Y4 belongs to a new trichoderma species. When the strain or a fermentation product thereof is applied to a rice culture medium, the growth of rice can be effectively promoted, and the plant biomass is increased. The trichoderma fungus Y4 is developed and prepared into the biological bacterial fertilizer, and a new way is provided for application of microorganisms to development of crop production.

The invention discloses a carbon-based decomposition agent for on-site returning of rape straws to field, and belongs to the technical field of microbial fertilizers. The carbon-based decomposition agent is prepared by mixing a microbial complex microbial inoculant, agricultural organic waste and biochar, wherein the microbial complex microbial inoculant is prepared by mixing bacillus subtilis, bacillus amyloliquefaciens, trichoderma asperellum, irpex lacteus and aspergillus niger; the biochar is formed by pyrolyzing a biomass raw material. According to the carbon-based decomposition agent for on-site returning of the rape straw to the field, rapid decomposition of the rape straw can be achieved, a good decomposition effect can also be achieved on substances with the component content similar to or lower than that of the rape straw, on-site returning of the rape straw to the field is effectively achieved, and sufficient nutrients are provided for next-stubble crops.

The application belongs to the technical field of Chinese medicine plant disease biological control, and particularly relates to a bacillus velezensis and application thereof. Bacillus velezensis The nsu-59 strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2025573. The bacillus velezensis provided by the application can not only prevent and treat the anthracnose of trichosanthes fruit, but also promote the seed germination of trichosanthes, and has a broad-spectrum antifungal effect on various Chinese medicine plant pathogenic fungi such as anthracnose, fusarium, trichoderma and diaporthe, has low prevention and treatment cost, is green and environment-friendly, will not cause drug resistance, and has great potential to be developed into a Chinese medicine plant biocontrol agent.

The invention discloses a multifunctional soil conditioner based on agricultural and forestry waste fermentation and a preparation method thereof, and relates to the field of soil conditioner preparation. The multifunctional soil conditioner based on agricultural and forestry waste fermentation is prepared from the following raw materials in parts by volume through two-stage fermentation: 60-80 parts of a fermentation main material, 10-25 parts of a functional auxiliary material, 5-15 parts of a complex microbial inoculant and 3-8 parts of a fermentation synergist, the fermentation main materials comprise seaweed residues, waste edible mushroom sticks and common agricultural and forestry wastes; the fermentation synergist is a compound of diatomite activated by a citric acid solution and humic acid raw powder; the complex microbial inoculant comprises a bacillus strain and a trichoderma strain in a specific ratio. Through the innovative compatibility of the seaweed residues and the waste mushroom sticks, the introduction of the fermentation synergist and the synergistic effect of the two-stage precise fermentation process, the defects in the prior art are effectively overcome, the fermentation efficiency and the product stability are remarkably improved, and efficient resource utilization of the agricultural and forestry residues is achieved.

This invention discloses a method for efficient utilization of straw resources and enhancement of soil carbon pool in grain-mushroom intercropping, comprising the following steps: preparing a layered substrate, the layered substrate comprising 40-45 parts by weight of wheat straw, 35-40 parts by weight of corn straw, 5-8 parts by weight of rice bran, 1-2 parts by weight of gypsum, and 0.5-1 parts by weight of calcium carbonate; subjecting the layered substrate to hydrothermal treatment at 65-70℃ for 90±5 minutes, and then cooling it to 25±2℃; inoculating the cooled substrate with Trichoderma harzianum strain T-22 at an inoculation amount of 10% of the substrate weight. 6 The concentration of CFU / g was measured and cultured at 25±2℃ and 75-85% relative humidity for 24-36 hours. A sequential inoculation system was used, first inoculating oyster mushrooms at 3% of the substrate weight, then inoculating shiitake mushrooms at 2.5% of the substrate weight after 7 days, and finally inoculating king oyster mushrooms at 2% of the substrate weight after another 7 days.

The application discloses a kind of potato green efficient fertilization methods, belong to agricultural planting technical field.The method includes: in sowing or land preparation, straw is promoted to rot microbial agent and antagonistic functional bacterium agent are mixed together with base fertilizer and are applied together;Before potato flowering period, antagonistic functional bacterium agent is mixed together with topdressing and is applied together.The straw is promoted to rot microbial agent and includes trichoderma and bacillus, and the antagonistic functional bacterium agent includes trichoderma, paenibacillus and streptomycete.The application can effectively improve potato yield and fertilizer utilization rate by the synergistic application of functional microorganism and fertilizer, while significantly reducing carbon footprint and nitrogen footprint in production process, solve the problem that high yield and environmental protection are difficult to consider in prior art, provide a popularized technical scheme for potato green efficient production.

The invention relates to a planting and cultivating method of high-yield dictyophora rubrovolvata, and belongs to the technical field of edible fungus planting, and the method comprises the following steps: selecting excellent hyphae, activating for 5-10 days, inoculating a glucose-agar rejuvenation culture medium, and culturing in a dark place; the wood chips, the pretreated bamboo chips, the auxiliary materials, the trichoderma inhibitor and the folium cortex eucommiae extract are mixed in proportion, the water content is adjusted, and sterilization and bagging are conducted; inoculating and spawn running: inoculating and culturing according to the inoculum size of 2-2.5% until the bag is full; and fruiting management: controlling the temperature, light, humidity and CO2 in stages, and harvesting until mushroom skirts are opened, mushroom holders are red and stems are 15-20cm long. According to the process, the stress resistance of the strain is improved, and the spawn running pollution rate is reduced; the culture medium is excellent in water retention and air permeability; the fruiting malformation rate is reduced, and the high-quality mushroom rate is increased. Compared with a traditional method, the infectious microbe contamination rate is remarkably reduced, and the total yield of dictyophora rubrovolvata and the proportion of high-quality mushrooms with complete mushroom skirts and thick and strong stipes are greatly increased.

The invention discloses a strain for degrading alternaria toxin and application of the strain, and relates to the technical field of microorganisms. The disclosed biocontrol bacterium MM14-1 is identified to be trichoderma harzianum through ITS, the trichoderma harzianum MM14-1 shows that the trichoderma harzianum MM14-1 has a good inhibition effect on growth of alternaria alternata in a degradation test on a liquid culture medium, the degradation rate reaches 96%, meanwhile, the degradation rate of an intracellular crude extract of the trichoderma harzianum MM14-1 on alternaria alternata toxin is 98.04%, and the trichoderma harzianum MM14-1 can be used for preparing the trichoderma harzianum. The intracellular crude extract of trichoderma harzianum MM14-1 can inhibit synthesis of alternaria tenuissima toxin, can be applied to prevention and treatment of alternaria tenuissima and alternaria tenuissima toxin, can overcome the problems of environmental safety, health safety and the like caused by use of chemical pesticides, is beneficial to pollution-free production of agricultural products, and can improve the product quality. According to the method, a strain capable of degrading alternaria toxin more efficiently, safely and stably is provided, and a breakthrough from bacterium prevention to detoxification is achieved.

The use of a composition comprising a Trichoderma fungus and a lignin fraction as a booster for nitrogen fertilizer is disclosed. Specifically, the composition has surprisingly been shown to enhance nitrogen uptake by plant seeds from nitrogen fertilizer. As a result, the total amount of the latter used can be advantageously reduced by at least 50% of the recommended amount.

The invention discloses a microbial traditional Chinese medicine compound preparation for preventing and treating watermelon fusarium oxysporum as well as a preparation method and an application method of the microbial traditional Chinese medicine compound preparation, and belongs to the field of pesticides, the compound preparation comprises bacillus velezensis, trichoderma harzianum, a traditional Chinese medicine extract, trehalose, chitosan and a dispersing agent, the preparation method comprises the following steps: mixing a high-activity bacterial liquid and a traditional Chinese medicine extract, adding trehalose, chitosan and a dispersing agent, and uniformly stirring to obtain the compound preparation, and during application, mixing 1-2kg of the compound preparation with soil per mu, so that the compound preparation remarkably enhances the prevention and treatment effect on soil-borne diseases through compounding of various traditional Chinese medicine extracts and microbial strains, and has a good application prospect. Growth of various pathogenic bacteria can be effectively inhibited, the disease occurrence rate is reduced, the preparation method is simple, cost is low, and large-scale production, popularization and application are easy.

This invention relates to high-protein-producing fungal strains belonging to the genera *Trichoderma*, *Aspergillus*, *Fusarium*, *Penicillium*, *Basilaria*, *Aureospora*, or *Pythium*, preferably *Trichoderma*, wherein the expression of at least one protein encoded by a gene selected from ID 65290, ID 120583, ID 43599, ID 35768, ID 80200, and ID 122457 or its ortholog is modified. The invention also relates to various uses of this strain and methods of genetic modification.

The invention discloses an iron powder coating for rice seeds directly seeded in workmanship, which comprises a rice seed body, the rice seed body is coated with an iron powder substrate layer, a water-sensitive bubble disintegration layer and a fungal spore active layer in sequence from inside to outside, and rice seed composite coated pellets are formed. Trichoderma fungi or endophytic fungi are selected as beneficial strains of the fungal spore active layer, and the problem that chemical agents and biological agents are incompatible is solved.

This invention discloses a compound microbial agent containing *Streptomyces longiflora* and *Trichoderma guiyuanensis*, wherein *Streptomyces longiflora* is *Streptomyces longiflora* A25 and *Trichoderma guiyuanensis* is *Trichoderma guiyuanensis* NJAU4742. This invention also discloses the application of the compound microbial agent in promoting cucumber growth and in increasing the yield and improving the fruit quality of seedless lychee. This invention uses *Streptomyces longiflora* A25 and *Trichoderma guiyuanensis* NJAU4742 isolated and screened from the rhizosphere soil of seedless lychee trees to prepare a compound microbial agent. When applied to cucumber pot experiments, it significantly promotes cucumber growth. When applied to seedless lychee field experiments, it significantly promotes the increase in seedless lychee yield and improves fruit quality. This compound microbial agent is of great significance to the sustainable development of the seedless lychee industry.