118 results about "Trichoderma" patented technology

The invention discloses a chelerythrine-containing compound microbial agent and application thereof, and belongs to the technical field of biological control. The invention provides a chelerythrine-containing compound microbial agent to solve the technical problems that in the prior art, only a single microbial agent and a plant extract are used, the biological control effect is poor, the stability is low, and the target spot is single. The compound microbial agent is prepared from a mortierella alpina extract, a trichoderma hairy extract and chelerythrine according to a specific compounding ratio. The compound microbial agent provided by the invention can delay the development of drug resistance of pathogenic bacteria through a multi-target effect, has better stability and stronger control effect, reduces the use amount of chemical pesticides, reduces the harm to the environment and human body, can be applied to tobacco planting production, can effectively reduce the control cost of tobacco black shank, and has broad application prospects. The economic benefit of tobacco planting is improved.

The invention provides a trichoderma maple fungicide, a microbial granular fertilizer as well as a preparation method and application of the trichoderma maple fungicide and the microbial granular fertilizer, and relates to the field of microorganisms. The acer rubrum fungicide contains acer rubrum RA with the preservation number of CGMCC No.35889. The acer rubrum fungicide has the capability of decomposing inorganic phosphorus, is wide in phosphorus dissolving range, can decompose tricalcium phosphate, ferric phosphate and aluminum phosphate, and particularly still has the very strong capability of decomposing the inorganic phosphorus under the low-temperature condition; the strain has the capability of producing siderophore and IAA at low temperature; the strain has an inhibition effect on various pathogenic bacteria causing field crop diseases. The microbial granular fertilizer provided by the invention contains various biostimulant substances, can promote crop growth, improve plant stress resistance and improve plant resistance to plant diseases and insect pests, and field tests prove that the microbial granular fertilizer can significantly promote crop growth and improve crop yield.

The invention relates to the technical field of agricultural planting, in particular to a method for preventing and treating watermelon fusarium wilt by using a green bactericide, which comprises the following steps: preparing the green bactericide which comprises the following components in percentage by mass: 18-25% of composite plant source active component consisting of water lettuce extract, enteromorpha polysaccharide and field horsetail flavone; 20%-30% of an antagonistic bacterium compound preparation, wherein the antagonistic bacterium compound preparation comprises bacillus pumilus and trichoderma; 38%-45% of a nano-carrier, wherein a chitosan-sodium alginate compound is used as an embedding material; and the balance of auxiliary components. Applying the green bactericide to watermelon plants; through component optimization and a synergistic effect, compared with the prior art, the composition has the remarkable advantages that the safety is high, all the components are from natural plants or microorganisms, no chemical pesticide residue exists, the composition is healthy to human bodies and environmentally friendly, and the problems of drug resistance and soil pollution caused by chemical agents can be avoided.

The invention provides a soil conditioner for improving soil hardening and a preparation method thereof, and relates to the technical field of soil conditioning. The soil conditioner is prepared by combining nano silicon dioxide, a straw retting material, a straw composite fermentation material, biochar, a water-retaining agent and modified starch. The straw composite fermented material is prepared by the following steps: crushing straws, mixing with sweet water, stirring, drying, carrying out microwave treatment, mixing with bacillus thuringiensis, fermenting, sterilizing, subsequently adding a composite microbial agent of bacillus subtilis, trichoderma and bacillus mucilaginosus, continuously fermenting, and drying. The modified starch is prepared by semi-gelatinizing corn starch and then carrying out microwave drying and shaping. According to the present invention, the defects in the prior art are overcome, the soil hardening phenomenon can be rapidly improved with the small addition amount, the soil porosity is increased, the activity and the diversity of the soil microorganisms are ensured, and the soil fertility is improved.

The invention relates to the technical field of agricultural biology, and discloses a microbial agent for preventing and treating fructus aurantii scab and a preparation method of the microbial agent. The microbial agent comprises the following raw materials in percentage by weight: 10-13% of paenibacillus mucilaginosus; 15%-20% of trichoderma sp. 7%-10% of paecilomyces lilacinus; 5%-8% of bacillus pumilus; 7%-9% of mycorrhizal fungi; 5%-8% of streptomyces; 5%-9% of a coptis chinensis extract; 4%-7% of a sophora flavescens extract; 2%-4% of realgar; 1%-3% of a rheum officinale extract; 10%-13% of soybean meal; 5%-8% of a water-soluble fertilizer; 7%-9% of potassium fulvate; 1%-3% of chitosan; 0.5%-1% of trace elements; 3%-8% of humic acid; the raw materials are subjected to microbial flora amplification culture, plant antibacterial component light-shielding mixing and low-temperature mixing to effectively protect the microbial activity of the microbial agent, and step-by-step operation can avoid damage of high temperature and mechanical shearing to microorganisms, so that the microbial activity of the microbial agent is effectively protected. And the loss of microbial active ingredients is reduced by vacuum drying and low-temperature granulation processes.

The invention discloses a complex microbial inoculant containing streptomyces elongisporus and trichoderma Guizhou, the streptomyces elongisporus is streptomyces elongisporus A25, and the trichoderma Guizhou is trichoderma Guizhou NJAU4742. The invention further discloses application of the complex microbial inoculant to promotion of cucumber growth and application to promotion of seedless litchi yield improvement and fruit quality improvement. Streptomyces elongisporus A25 and trichoderma Guizhou NJAU4742 which are separated and screened from seedless litchi tree rhizosphere soil are prepared into the composite microbial agent, the composite microbial agent is applied to a cucumber pot experiment, cucumber growth can be remarkably promoted, and when the composite microbial agent is applied to a seedless litchi field experiment, seedless litchi yield improvement and fruit quality improvement can be remarkably promoted. The complex microbial inoculant disclosed by the invention has important significance on sustainable development of the seedless litchi industry.

The invention discloses a preparation method of a tomato culture medium, which aims at solving the technical problems that peat resources are non-renewable and high in cost and the utilization rate of mulberry twig wastes is low, and is realized by the following technical scheme: firstly, crushing fresh mulberry twigs into particles; mixing silkworm excrement with a sodium alginate solution, and coating the mulberry twig particles in a fluidized bed at the air temperature of 60 DEG C and the atomization pressure of 0.3 MPa to form core-shell structure particles with the shell thickness of 0.1-0.3 mm; then mixing the core-shell particles with fine peat to form a mixture, and stacking the mixture with the coarse peat and the fine peat to form a three-layer gradient structure; and finally, spraying a zymophyte liquid with trichoderma content greater than or equal to 1.0 * 10 < 7 > CFU / mL until the water content is 40%, and standing and activating for 20 days. The matrix takes mulberry twigs as a core raw material, combines silkworm excrement slow-release fertilizer efficiency and gradient structure optimization water-air balance, can replace a traditional peat matrix to be used for tomato planting, and realizes agricultural waste recycling and low cultivation cost.

The invention belongs to the field of fungus secondary metabolites and application thereof, and particularly relates to trichoderma reesei HK1-12 as well as a secondary metabolite and application thereof, the collection unit is China General Microbiological Culture Collection Center (CGMCC), and the collection number is CGMCC No. 41951. The trichoderma HK1-12 has excellent characteristics in the aspects of growth promotion, plant protection and the like, and is expected to replace the existing similar products to be popularized and applied.

The invention relates to the technical field of animal feed, and particularly discloses a bamboo powder dietary fiber probiotic feed additive as well as a preparation method and application thereof. The additive consists of bamboo powder dietary fibers, a probiotic agent and a carrier, wherein probiotics are at least one of trichoderma, bacillus, lactic acid bacteria and saccharomycetes. The preparation method comprises the steps of bamboo powder pretreatment, solid state fermentation, drying, mixing and the like. The product fully utilizes the prebiotics characteristics of the bamboo powder and the regulation effect of probiotics, can effectively improve the intestinal health of livestock and poultry, improve the feed utilization rate and reduce the breeding cost, can partially or completely replace antibiotics, and has the characteristics of greenness, safety and high efficiency.

The invention discloses a treatment method of fermentation wastewater. The treatment method of the fermentation wastewater comprises the following steps: adding filamentous fungi and green algae into fermentation wastewater generated after fermentation treatment of kitchen garbage, carrying out fermentation culture, then carrying out solid-liquid separation, and extracting grease from the separated solid. The filamentous fungi comprise trichoderma harzianum, and the green algae comprise one or a combination of more selected from chlorella vulgaris, chlamydomonas, haematococcus pluvialis, hylothrix, scenedesmus obliquus and chaetoclada. According to the treatment method of the fermentation wastewater, grease can be produced while the fermentation wastewater is treated, and the treatment cost of the fermentation wastewater is reduced.

The application discloses a kind of trichoderma fermentation processing indigo blue method, the product obtained and application.The method includes: preparation trichoderma seed liquid;After primary indigo blue sterilization, access seed liquid, fermentation 5-9 days under 25-30 DEG C, 120-180 rpm, avoid light, obtain secondary processing indigo blue.The application first utilizes trichoderma to carry out biological transformation to indigo blue, can significantly improve the content of its key anti-tumor component indigo carmine with specificity, and the increase is more than 150%.In vitro anti-tumor experiment shows that the inhibitory activity of secondary processing indigo blue prepared by the application to human leukemia K562 cells and human colon adenocarcinoma cell SW480 cell is significantly better than ordinary indigo blue.The product of the application can be used for preparing medicine for preventing and / or treating leukemia, colon adenocarcinoma, process controllable, suitable for industrialized production, and provides a new way for developing efficient indigo blue anticancer new drug.

This invention belongs to the field of biological agents and plant breeding technology, specifically relating to the application of *Trichoderma tumefaciens* T-51 in promoting flowering, fruit setting, and vegetative growth in plants. The *Trichoderma tumefaciens* T-51 described in this invention has the preservation number CCTCCNO: M2015729. Applying a suspension containing *Trichoderma tumefaciens* T-51 to the roots of plant seedlings can advance the flowering time and increase the number of flowers; it can also improve the number and rate of fruit set; and it can promote vegetative growth, especially increasing plant height, number of stem nodes, and number of leaves, thereby accelerating the plant breeding process and shortening the breeding cycle. This invention has very high application value in plant breeding.

This invention relates to the field of soil remediation technology, specifically disclosing a method for remediating heavy metal-contaminated soil using microbial agents. The invention uses hydroxyapatite as a carrier, utilizing its adsorption properties to load licorice extract onto its surface. To further improve the stability of the licorice extract, a carboxymethyl cellulose (CMC) solution is added. After stirring, mixing, and drying, the excellent film-forming properties of CMC help improve the stability of the licorice extract, while also increasing the specific surface area and adsorption sites of the hydroxyapatite. The introduction of licorice extract and CMC not only enhances the chelating ability of hydroxyapatite for heavy metals but also provides an additional carbon source for microorganisms, promoting their growth and reproduction. The metabolic activity of *Trichoderma solani* and *Bacillus licheniformis* is significantly enhanced, resulting in a substantial increase in remediation efficiency.

This invention discloses a method for processing bamboo shoot residue that synergistically enhances the content of soluble dietary fiber and its bound polyphenols. Using waste bamboo shoot residue as raw material, the fermentation bacteria are all food-grade. Specifically, a multi-strain coupled fermentation is employed, including *Bacillus belye*, *Bacillus natto*, *Trichoderma kangsei*, *Cladosporium*, *Aspergillus pamoateus*, and *Bacillus coagulans*. This significantly alters the ratio and content of soluble and insoluble dietary fiber in the bamboo shoot residue and substantially increases the content of dietary fiber-bound polyphenols, synergistically enhancing the content of soluble dietary fiber and its bound polyphenols, thereby producing high-quality dietary fiber. This invention is simple to operate, low in cost, and allows for easier control of fermentation conditions. It is highly efficient and does not easily damage the non-covalent bonds (hydrogen bonds, electrostatic or ionic bonds, etc.) or covalent bonds (ester bonds, ether bonds, etc.) binding dietary fiber and polyphenols. It also has no significant impact on the pH and temperature of the environment, and can significantly increase the content of soluble dietary fiber and its bound polyphenols in bamboo shoot residue.

This invention belongs to the field of bio-mineral fertilizer technology, specifically relating to a microbial liquid compound agent and bio-mineral fertilizer that combine coal gangue activation and heavy metal remediation. The microbial liquid compound agent comprises the following functional strains prepared through three-stage sequential inoculation and fermentation using electrochemical low-Hertz water: *Pseudomonas putida* (a remedial bacterium), *Azotobacter chrysogenum* (a nitrogen-fixing bacterium), *Azospirillum brasiliensis* (a growth-promoting bacterium), *Pseudomonas fluorescens* (a phosphate-solubilizing bacterium), *Bacillus subtilis* (a biocontrol bacterium), *Bacillus mucilaginosus* (a potassium-solubilizing bacterium), *Bacillus megaterium* (a silica-solubilizing bacterium), and *Trichoderma echinococcus* (a synergistic bacterium). The bio-mineral fertilizer is prepared using the aforementioned microbial liquid compound agent, with coal gangue powder as the main raw material. The scientific combination of different strains provided by this invention achieves efficient improvement of heavy metal-contaminated land and increased crop yields. Simultaneously, it transforms coal gangue into a valuable resource, solving the pollution problem caused by the large-scale accumulation of coal gangue, and possesses significant economic and social value.

The invention discloses a LeRho1 gene expression vector and a method for improving heat resistance and trichoderma resistance of lentinus edodes. The LeRho1 gene expression vector is a recombinant expression vector, a target gene for coding the LeRho1 protein is inserted into the recombinant expression vector, and the amino acid sequence of the LeRho1 protein is shown as SEQ ID NO. 1. The invention finds that the LeRho1 gene has a positive regulation effect on the heat resistance of the shiitake mushroom strain, and the LeUSPA and LeUSPH genes mediate the thermal response of the shiitake mushroom strain through negative regulation. Therefore, according to the method, the LeRho1 gene overexpression vector or LeUSPA and LeUSPH gene silencing vectors are constructed and transferred into the shiitake mushroom strain for genetic transformation, the heat resistance and trichoderma resistance of the shiitake mushroom strain can be remarkably improved, and the transformation efficiency is high.

The present invention discloses a method for preparing β-glucose oligosaccharides, and relates to the field of fermentation technology. The method comprises the following steps: (1) preparing a Bacillus faecalis seed solution; (2) preparing a Trichoderma LE02 seed solution; (3) fermenting: inoculating the Bacillus faecalis seed solution into a fermentation medium, and inoculating the Trichoderma LE02 seed solution once at 8-12 hours, 45-55 hours, and 85-95 hours after the inoculation of the Bacillus faecalis seed solution, respectively. The method is ventilated and fermented for 120 hours to obtain a β-1,3-glucose oligosaccharide fermentation liquid; and (4) purifying. The present invention, by extending the fermentation time or adding the Trichoderma LE02 seed solution in batches, ultimately obtains a thermogelling oligosaccharide β-1,3-glucose oligosaccharide with a lower degree of polymerization and a higher concentration, with a yield of up to 89-93%, which can be industrialized.

The invention relates to the technical field of vegetable disease and pest control, and discloses a green vegetable disease and pest control method based on a microbial preparation, which comprises the following steps: selecting an application mode according to a target disease and pest type and a vegetable growth stage; a microbial preparation working solution or a mixture is prepared, the microbial preparation contains beneficial microorganisms, and the beneficial microorganisms comprise one or more of bacillus subtilis, trichoderma, paecilomyces lilacinus, metarhizium anisopliae or beauveria bassiana. According to the green prevention and control method for vegetable diseases and insect pests based on the microbial preparation, the adopted microbial preparation mainly comprises beneficial microorganisms such as bacillus subtilis, trichoderma and paecilomyces lilacinus and natural metabolites of the beneficial microorganisms and does not contain any chemical synthetic pesticide, and no toxic and harmful substance residues exist after the method is used; the pollution of traditional chemical pesticides to soil, water sources and air is fundamentally avoided, the farmland ecological environment is effectively protected, and the pesticide is safe to non-target organisms such as pollination insects and soil beneficial organisms.

The invention discloses a preparation method of a co-culture metabolite carbon-based biostimulant based on different combinations of trichoderma, bacillus and metarhizium anisopliae. Compared with a single microorganism culture metabolite carbon-based preparation, the metabolite carbon-based preparation co-cultured by different combined micro-substances has obvious synergism in the aspects of wheat growth promotion and stress resistance improvement; the optimal proportion and time of the metabolite co-cultured by different combinations of the trichoderma, the bacillus velezensis and the metarhizium anisopliae adsorbed by the rice straw biochar are obvious in synergistic interaction in promoting wheat seed germination and seedling growth and improving wheat stress resistance. Different combinations of co-culture metabolites and biochar compounded preparations can be developed into novel carbon-based biostimulant, and the carbon-based biostimulant has important significance in promoting growth of wheat and corn, improving stress resistance and disease resistance and the like.

The invention belongs to the technical field of fertilizer preparation, particularly relates to a preparation method of a functional medium trace element fertilizer, and aims to solve the problems that in the existing fertilizer preparation process, a coating layer usually only pays attention to physical slow release and lacks the function of synergistically providing active substances (such as amino acid and polysaccharide) and trace elements; in order to solve the problem that the conventional addition mode of trace elements is difficult to ensure the stability and bioavailability of the feed, the invention provides the following scheme: the preparation method comprises the following steps: S1, preparing materials: 15-30 parts of straws, 5-15 parts of soybean meal and 3-10 parts of molasses; through use of the straw, the soybean meal, the molasses, the bacillus subtilis, the nitrogen-fixing bacteria, the phosphate solubilizing bacteria, the trichoderma, the nitrogen-phosphorus-potassium fertilizer, the humic acid, the ferrous sulfate, the zinc sulfate, the manganese sulfate, the boric acid, the sodium molybdate, the sodium alginate, the chitosan and the serine, the problems of single coating function, poor trace element stability and low bioavailability in the fertilizer preparation process are effectively solved.

The invention belongs to the technical field of biocontrol bacteria. The invention relates to trichoderma aureobasidium, in particular to trichoderma aureobasidium antagonistic to wood-rotting fungi and epidemic blight and application of the trichoderma aureobasidium. The invention provides a Trichoderma aureobasidium P21-3 separated from soil, which is preserved in Guangdong Microbial Culture Collection Center on September 5, 2025, and the preservation number of the Trichoderma aureobasidium P21-3 is GDMCC No: 66930. The compound has a remarkable inhibiting effect on various wood-rot pathogenic bacteria and epidemic disease pathogenic bacteria of elaeagnus coriolis, including ganoderma sinense, phellinus xylaris harmful, cryptophyllothecium elaticum and the like, and the antibacterial rate reaches 80% or above; the method has a strong prevention and control capability on the durian epidemic disease which outbreaks in recent years and does not have an efficient prevention and control means. As a natural biocontrol fungus, the P21-3 strain and its fermentation broth / metabolite have the advantages of environmental friendliness, ecological safety, no chemical residue and the like, can effectively replace or significantly reduce the use of traditional chemical bactericides, is an excellent biocontrol fungus resource, has significant ecological benefits, social benefits and economic benefits, and has wide industrial application prospects.

The invention belongs to the technical field of fertilizers. The invention provides an organic fertilizer based on abandoned citrus fruits and a preparation method thereof.The preparation method comprises the steps that S1, 1 ton of abandoned citrus fruits, 265-275 kg of wheat straw and 10-12 kg of soybean meal are mixed to be uniform, and a base material is obtained; s2, inoculating compound bacteria liquid into the base material obtained in the step S1 to obtain a fermented material; the composite bacteria liquid comprises trichoderma spore suspension, bacillus subtilis liquid and nitrogen-fixing bacteria liquid, and 0.5-0.6 L of trichoderma spore suspension, 1 L of bacillus subtilis liquid and 0.4-0.5 L of nitrogen-fixing bacteria liquid are used for every 100 kg of the base material; and S3, carrying out composting fermentation on the fermented material obtained in S2 to obtain the organic fertilizer based on the abandoned citrus fruits. The composting degree of the finally prepared organic fertilizer can be effectively improved.

The invention discloses application of Cladosporium halotolerans in plant growth promotion, and belongs to the technical field of microorganisms, the Cladosporium halotolerans is Cladosporium halotolerans 550W1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.41943. The invention further discloses a preparation method of the Cladosporium halotolerans. The application of the Cladosporium halotolerans in plant growth promotion belongs to the technical field of microorganisms. According to the salt-tolerant cladosporium sp. 550W1 provided by the invention, plant growth can be promoted by synthesizing auxin, gibberellin, cytokinin and other plant hormones; meanwhile, the salt-tolerant cladosporium sp. 550W1 has the outstanding advantages of being high in environmental adaptability, environmentally friendly, high in safety and the like, and an excellent core strain resource is provided for developing novel microbial fertilizers and promoting green sustainable agriculture.

This invention relates to a microbial composition for preventing and controlling root rot in tea trees and its application, belonging to the field of microbial and organic fertilizer technology. The composition comprises the following components: Component 1, Bacillus kaempferi and Trichoderma yunnanensis; Component 2, humic acid, Chrysanthemum indicum extract, amino acids, brown algae oligosaccharides, complex vitamins, and trace elements; wherein, Bacillus kaempferi is N4471 (…). Bacillus cabrialesii The accession number is CCTCC NO: M20221660, and the Yunnan Trichoderma is YNK-FG0001 ( Trichoderma yunnanense The microbial composition, with accession number CCTCC NO: M 2025645, significantly improved soil water stability aggregates, pH, and cation exchange capacity when applied to tea plant cultivation. It also enhanced root vitality and the activity of antioxidant enzymes [superoxide dismutase (SOD) and peroxidase (POD)], thereby reducing the growth of Fusarium rot (…). Fusarium solani ), Pythium ( Pythium spinosum ) and teosinte ( Rosellinia arched The disease index of tea tree root rot caused by infection was used to ultimately achieve effective control of tea tree root rot.

The invention relates to an isolated bacteriophage that is aimed at Xylella fastidiosa, wherein the bacteriophage: (a) is capable of inhibiting the growth of said Xylella fastidiosa; (b) comprises a short non-contractile tail and an icosahedral capsid; (c) exhibits a genomic size of about 42000 bp to 45000 bp; and (d) comprises a genome with a DNA sequence having at least 72% sequence identity to SEQ ID NO:1.

The invention relates to a microbial composite bacterial agent, which is prepared from Klebsiella sp. CS11, Trichoderma longibrachiatum and Azotobacter chroococcum according to a mass ratio of 1: 1: 1, the classification name of the bacterial strain is Klebsiella sp. CS11, and the preservation number of the bacterial strain is CCTCC M 20242943. When the Klebsiella CS11 strain is applied to aerobic composting of corn straw and chicken manure, H2S gas emission can be reduced, and the sulfur metabolism efficiency in the composting process can be improved. On the basis, the composite microbial agent further accelerates decomposition and conversion of organic matters, reduces H2S gas emission and improves sulfur fixation efficiency, meanwhile, the content of amino acid, humic acid and the like of compost is adjusted, high-quality organic fertilizer is produced, the soil environment is improved, cyclic utilization of resources is achieved, and agricultural sustainable development is promoted.

The invention relates to the technical field of special fertilizer effect compositions, and particularly discloses a seedling raising and plant promoting composition for mountain mangoes and a rapid production and field planting method. The composition comprises various components such as microbial metabolite fermentation liquor dry powder, trichoderma fermentation filter residue powder, tea bran residue crushed matter, perlite loaded chitosan oligosaccharide composite particles, a kaolin-sepiolite compound and sodium alginate gel particles, and the core components are matched and coordinated to construct a stable seedling environment structure. The invention further provides a rapid production and field planting method matched with the composition, the whole process of seedling raising, bag transferring, shaping and management before outplanting is covered, and the method is suitable for intensive cultivation and efficient transplanting of mangoes under complex mountain conditions and has good technical adaptability and application prospects.

The present application belongs to the field of microorganisms and biological control technology, and particularly relates to Burkholderia cenocepacia Tzs39 and application thereof in preventing and treating plant pathogenic fungi. The present application provides a Burkholderia cenocepacia Tzs39, and the preservation number of the Burkholderia cenocepacia Tzs39 is CGMCC No.30082. The antagonistic bacteria Tzs39 in the present application is isolated from rhizosphere soil of continuously cultivated Radix Pseudoginseng, has a good rhizosphere colonization effect and is safe and reliable, and can be used for preventing and treating or alleviating soil-borne disease problems of continuously cultivated medicinal plants, preventing and treating root rot in the process of continuously cultivating medicinal plants. The results of the embodiments show that the antagonistic bacteria Tzs39 can effectively inhibit the growth of Fusarium oxysporum and Trichoderma.

This invention belongs to the field of biological agent technology, specifically relating to a strain of *Trichoderma*, a biocontrol agent, a bio-organic fertilizer, and methods and applications for controlling plant diseases. This invention provides a strain of *Trichoderma* P11, with accession number CCTCC NO: M 2024648. The *Trichoderma* P11 of this invention can enhance positively correlated disease resistance genes in plants. PnCAD , PnSN1 , PnDEFL1 The relative expression level of *Trichoderma* P11 was increased, enhancing the activity of disease resistance-related enzymes SOD, POD, and CAT. Furthermore, *Trichoderma* P11 could damage the cell membrane of pathogens, inhibiting their growth. *Trichoderma* P11 also exhibits good rhizosphere colonization ability. The results of the examples show that *Trichoderma* P11 of the present invention can reduce the incidence of root rot in Panax notoginseng fields and can also antagonize various pathogens such as *Pseudomonas aeruginosa*, *Pseudomonas oryzae*, *Geotrichum candida*, *Early Blight of Tomato*, *Phytophthora indicum*, *Phytophthora capsici*, and *Alternaria spiralis*.