233 results about "Microorganism resource" patented technology

The invention discloses Trichoderma longibrachiatum NT241 and application thereof, the microbiological classification name of the Trichoderma longibrachiatum NT241 is Trichoderma longibrachiatum NT241, the Latin literature name of the Trichoderma longibrachiatum NT241 is Trichoderma longibrachiatum NT241, the preservation number of the Trichoderma longibrachiatum NT241 is CGMCC (China General Microbiological Culture Collection Center) No.41139, and the preservation address of the Trichoderma longibrachiatum NT241 is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The trichoderma longibrachiatum NT241 disclosed by the invention grows quickly, the generated endo-beta-glucanase and manganese peroxidase are relatively high in activity, the degradation of straws of crops such as oilseed rape and wheat is promoted by adding the liquid fungicide, and an efficient microbial resource can be provided for straw returning utilization.

The invention discloses a biocontrol complex microbial inoculant for preventing and treating maize curvularia leaf spot and application thereof, and belongs to the field of crop disease prevention and treatment. The biocontrol complex microbial inoculant comprises the following active ingredients: bacillus cereus, pseudomonas chlororaphis and streptomyces microflavus, and / or metabolites thereof. The beneficial microorganisms with different action mechanisms are combined, and the prevention and control effect on the curvularia maize leaf spot disease is enhanced through the synergistic effect. Natural microbial resources are adopted to replace traditional chemical pesticides, harmful substance residues are reduced, and the ecological environment is protected. Through reasonable matching of different types of beneficial microorganisms, effective prevention and control of the curvularia maize leaf spot disease are realized, and healthy growth of plants can be promoted. Compared with a single strain preparation, the complex microbial inoculant provided by the invention has stronger stability and durability, and can significantly reduce the incidence rate of diseases.

The invention relates to an ecological restoration method for a high and cold mine, and belongs to the technical field of ecological restoration. The ecological restoration method for the high-cold mine comprises the step of performing ecological restoration on the high-cold mine by adopting vegetation configuration of shrubs, herbaceous plants A and herbaceous plants B, the herbaceous plants A comprise at least four of elymus dahuricus, siberian wildrye herb, festuca arundinacea, bluegrass, stipa purpurea, ryegrass, frozen artemisia stelleriana and herba artemisiae annuae; the herbaceous plant B comprises at least three of artemisia desertorum, plantain herb, alfalfa, astragalus membranaceus, dandelion and oxytropis philippinarum; the shrub species comprise at least two of sophora moorcroftiana, sabina vulgaris, sea-buckthorn, caragana and buddleia. According to the method, specific vegetation configuration of shrubs, herbaceous plants A and herbaceous plants B is adopted, and soil microorganism resources are recovered quickly; the extreme environment of high and cold mines can be overcome; the ecological system is quickly and efficiently restored, only one-time sowing is needed, and the cost is low; the vegetation and microorganisms interact with each other, so that the survival rate of the vegetation is high.

The invention discloses alkali-producing cadmium-tolerant acinetobacter capable of increasing the pH value of soil and application of the alkali-producing cadmium-tolerant acinetobacter, and belongs to the technical field of microorganisms, the alkali-producing cadmium-tolerant acinetobacter is preserved in the China General Microbiological Culture Collection Center (CGMCC), the number of the alkali-producing cadmium-tolerant acinetobacter is CGMCC No.33619, and the alkali-producing cadmium-tolerant acinetobacter belongs to proteobacteria and pseudomonas. The strain can increase the environmental pH value from 6.0 to 8.0 through metabolism, and stably grows in an environment with the cadmium (Cd < 2 + >) concentration as high as 20 ppm. The strain can be applied to the fields of acid soil improvement, cadmium pollution remediation, industrial wastewater treatment, fermentation engineering and the like. The method has the advantages of being environmentally friendly, efficient, stable, applicable to multiple scenes and the like, and a novel microbial resource is provided for heavy metal pollution treatment and soil ecological restoration.

The invention discloses a Pandoraea sp. Capable of efficiently degrading aureomycin and application of the Pandoraea sp. The strain is a strain FJNUIM-SW2, the classification name of the strain is Pandoraea nomenusa, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation time is October 17, 2024, the preservation number is CGMCC NO.32242, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing. The strain FJNUIM-SW2 can grow by taking aureomycin as the sole carbon source and energy, has a good aureomycin degradation characteristic, can be used for microbial degradation of aureomycin in environments such as aureomycin wastewater and soil, provides microbial resources for bioremediation of aureomycin in the environment, and has potential application value in ecological environment protection.

The invention belongs to the technical field of microbial resource development and straw biological decomposition. The invention discloses cold-resistant pseudoarthrobacter psychrotolans CKON-4, and the preservation number of the cold-resistant pseudoarthrobacter psychrotolans CKON-4 is CGMCC (China General Microbiological Culture Collection Center) No.29378. The cold-resistant pseudoarthrobacter psychrotolans CKON-4 has the advantages that the preservation number The strain CKON-4 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 25, 2023, the China General Microbiological Culture Collection Center is called CGMCC for short, the address of the China General Microbiological Culture Collection Center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number of the strain is CGMCC No.29378. The strain disclosed by the invention has very high lignocellulose degradation activity, and the decomposition rate of straws can be remarkably improved; the strain is separated from rice field soil, can grow in the pH value range of 5-9 and the temperature range of 4-37 DEG C, can tolerate the salt concentration of 10%, and is a bacterium with wide pH application range, salt resistance and cold resistance. The strain can play an important role in lignocellulose degrading enzyme production, straw biological decomposition and compost fermentation.

The invention belongs to the technical field of biological prevention and control of plant diseases, and provides bacillus subtilis and application thereof in prevention and control of corn diseases. The preservation number of the bacillus subtilis SEB-34 is CGMCC (China General Microbiological Culture Collection Center) No. 26419. The invention also provides a microbial agent produced by using the SEB-34 strain, and the microbial agent can be used for preventing and treating plant diseases caused by fusarium graminearum or pelamys maydis. The bacillus subtilis provided by the invention is applied through seed treatment before sowing or root drip irrigation after seedling emergence, has the effects of preventing diseases, promoting growth and increasing yield, and can effectively reduce the occurrence of corn southern leaf blight and basal stem rot. The microbial agent provided by the invention is in a semi-dormant state, has relatively strong stress resistance, is more stable to store, ensures the effect, and effectively reduces the production and transportation cost at the same time. The invention also provides beneficial microbial resources for green prevention and control of corn diseases, and has good application prospect and high application value.

The invention discloses a desertification region soil ecology improvement and restoration system which comprises a multifunctional biochar preparation module used for collecting desertification region crop waste and preparing biochar through high-temperature pyrolysis to serve as a soil conditioner; the microbial remediation module is used for selecting drought-enduring plant species and culturing soil microorganisms; the resource comprehensive utilization module is used for collecting rainwater and establishing a water storage system; and the soil remediation device comprises a fertilization unit, an irrigation unit, a soil softening unit and a vegetation planting unit. Soil improvement is achieved through fertilization, irrigation and soil softening, the survival rate of later plants is increased, power is supplied to the soil remediation device in cooperation with the comprehensive resource utilization module, the utilization rate of renewable resources is increased, and therefore the ecological relation of mutual promotion among soil, vegetation and microorganisms is constructed; the resource comprehensive utilization module and the soil remediation device can guarantee sustainable development of the ecological relationship, and soil ecology improvement and remediation in the desertification area are achieved.

The invention discloses application of bacillus subtilis BY-S1 in preparation of biological nano-selenium, and belongs to the technical field of microbiology and biological nano-selenium preparation. The preservation number of the bacillus subtilis BY-S1 is CCTCC (China Center for Type Culture Collection) NO: M 20232368. Research finds that the bacillus subtilis BY-S1 has the function of converting and synthesizing the biological nano-selenium by utilizing selenite, the strain BY-S1 and the biological nano-selenium converted and synthesized by the strain BY-S1 can remarkably promote plant growth, and when the bacillus subtilis BY-S1 and the biological nano-selenium are combined for use, a synergistic interaction effect is achieved. A new microbial resource is provided for biosynthesis of nano-selenium, a stable, safe and efficient nano-selenium preparation method is provided, and a theoretical basis is laid for research of a novel plant growth promoter.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating idesia anthracnose, fermentation liquor, a biocontrol bacterium agent and application. According to the invention, a biocontrol bacterium is separated and screened from microbial resources on healthy idesia leaves, is classified and named as Bacillus velezensis, is preserved in China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided by the invention has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, botrytis cinerea, fusarium oxysporum, alternaria alternata and pestalotiopsis which can cause plant diseases, and also has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, fusarium oxysporum, alternaria alternata and pestalotiopsis. The biocontrol bacterium, the fermentation broth and the biocontrol inoculant provided by the invention have potential application prospects in prevention and treatment of idesia anthracnose.

The invention provides a Brevibacillus formmosus strain SXM-17, a derivative product of the Brevibacillus formmosus strain SXM-17 and application of the Brevibacillus formmosus strain SXM-17 to prevention and control of crop diseases, and belongs to the technical field of functional microorganisms. The strain SXM-17 is separated and purified from wheat plants, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.30198, and the strain SXM-17 has a good inhibition effect on gibberellic disease bacteria, physalospora piricola, botrytis cinerea, alternaria solani, stem rot bacteria, gaeumannosis and the like. Experiments show that a culture solution, a bacterial suspension and a supernatant of the strain SXM-17 have good prevention and treatment effects. In addition, the strain SXM-17 also has a growth promoting effect on crops planted in a natural planting environment and a salinization environment. The strain SXM-17 provided by the invention enriches a gibberellic disease biocontrol strain resource library and green and efficient biological control microbial resources.

The present invention belongs to the field of microbial technology and specifically relates to a salt-tolerant growth-promoting composite bacterial agent and its application. The active ingredients of the salt-tolerant growth-promoting composite bacterial agent are actinomycete S36, deposited with CGMCC No. 32928, Trichoderma M2, deposited with CGMCC No. 41651, and Bacillus velezensis Y10, deposited with CGMCC No. 32927. The present invention screened and obtained a composite bacterial agent obtained by mixing these three bacteria, which can improve the resistance of cotton and wheat to salt stress, providing a new microbial resource for improving the salt tolerance of cotton and wheat.

The invention relates to the technical field of microorganisms, and discloses a pseudomonas putida strain and a preparation method and application thereof.The strain is pseudomonas putida which is separated from soil and has the capacity of dissimilatory reduction of nitrate into ammonium and is classified into a new pseudomonas putida strain based on GTDB-TK. A dissimilatory NOreductive gene cluster, namely an nirBD gene cluster, exists on a genome of the gene, nirK / nirS genes are deleted, and NOs can be reduced into NH under the anaerobic culture condition. According to the application of the pseudomonas putida strain in improvement of soil nitrogen management, the pseudomonas putida strain can be mixed with organic fertilizer for use so as to enhance the effective utilization and conversion capacity of nitrogen in soil. By means of the pseudomonas putida strain, NON can be reduced into NHH under the anaerobic condition, the culture method is simple, the growth speed is high, variation is not prone to occurring, and a brand new microbial resource is provided for soil nitrogen cycle research.

The invention discloses a micro-plastic degrading bacterium P.ataveovii and application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of Priestia aryabhattai BK12 is separated and screened from plastic wastes, and the plastic degradation capability of the Priestia aryabhattai BK12 is deeply explored. A research result shows that the P.avenae BK12 can be used for efficiently degrading the PET (Polyethylene Terephthalate), and the P.avenae BK12 can be used for efficiently degrading the PET (Polyethylene Terephthalate), so that the P.avenae BK12 can be used for efficiently degrading the PET (Polyethylene Terephthalate). The invention provides a new microbial resource for the development of a plastic degradation agent, contributes to promoting the development of a biodegradable plastic technology and solving the problem of environmental pollution caused by PET, and assists in realizing the goal of green and sustainable development.

The invention discloses gluconobacter oxydans for leaching waste printed circuit boards and a leaching method of the gluconobacter oxydans, and belongs to the technical field of microbial resource recovery treatment, lixivium for leaching extraction comprises the gluconobacter oxydans, metabolites of the gluconobacter oxydans and an H2O2 solution, and the H2O2 solution is added to improve the metal leaching percentage and shorten the leaching time. Compared with traditional chemical and biological leaching, efficient leaching of the metal copper in the waste printed circuit board is achieved under the relatively mild condition, safety is higher, and secondary pollution to the environment can be relieved.

The invention discloses bacillus velezensis TG1 and application thereof. The bacillus velezensis TG1 is classified and named as bacillus velezensis, and the preservation number of the bacillus velezensis TG1 is GDMCC 66317; the preservation date is May 12, 2025, and the preservation unit is Guangdong Microbial Culture Collection Center. The bacillus velezensis TG1 provided by the invention has broad-spectrum antibacterial activity, high environmental adaptability and high safety, provides a green, sustainable, safe and efficient microbial resource for aquaculture, and has important ecological and economic values.

The invention belongs to the technical field of microbial strains and environmental microbiology, and discloses a novel strain capable of degrading tetracycline antibiotics. The new bacterial strain is an Enterobacter hormaechei SD7-1 bacterial strain, and is preserved in the Guangdong Microbial Culture Collection Center on March 3, 2025, and the preservation number is GDMCC No: 65849. The invention further discloses a preparation method of the new bacterial strain. The new strain provided by the invention has a remarkable degradation effect on tetracycline antibiotics, and the degradation rate of tetracycline after 24-hour treatment reaches 50% or above. Therefore, as a functional strain, the enterobacter hormaechei SD7-1 strain has high degradation efficiency and a wide degradation spectrum, can effectively degrade various tetracycline antibiotics in a short time, and has the advantages of treating pollution caused by wide use of tetracycline, further reducing environmental half-life emission of tetracycline, protecting the environment, and having good application prospects. The invention provides a novel microbial resource with potential in soil and water environments, especially in soil and water environments.

The present invention belongs to the field of microbial technology, and specifically relates to a schizochytrium with high EPA production and its application. The present invention obtains a schizochytrium for fermenting and preparing EPA through ultraviolet mutagenesis and selective culture screening, and the strain name is Schizochytrium sp.ALS01. The schizochytrium ALS01 can produce high-yield EPA through fermentation in ordinary culture medium, with an EPA content of up to 10.11%. It can also produce high-yield DHA, with a DHA content of up to 46.02%, and is an ideal microbial resource for producing a variety of ω-3 fatty acids. At the same time, the schizochytrium ALS01 with high EPA and DHA production can not only reduce the production cost of EPA and DHA, but also meet the current growing demand for EPA, which is conducive to promoting the promotion and application of schizochytrium production technology of EPA and DHA.

The invention discloses staphylococcus Z8 for antagonizing pathogenic bacteria of mulberry bacterial wilt and application of the staphylococcus Z8. The Z8 is identified as staphylococcus sp. And is preserved in Guangdong Microbial Culture Collection Center, the preservation number is GDMCC No.66232, and the preservation time is April 28, 2025. The invention further discloses a preparation method of the staphylococcus Z8 for antagonizing the pathogenic bacteria of mulberry bacterial wilt and application of the staphylococcus Z8 for antagonizing the pathogenic bacteria of mulberry bacterial wilt. The strain Z8 disclosed by the invention can destroy the formation of a biological membrane of the mulberry ralstonia solanacearum, so that the pathogenicity of the ralstonia solanacearum is reduced, and the strain Z8 can be used for preventing and treating the mulberry bacterial wilt. In addition, the strain Z8 does not affect the growth and development of the mulberry, the safety of the strain as a biocontrol strain is embodied, and the root activity and the total chlorophyll content can be improved. An efficient and environment-friendly microbial resource is provided for biological prevention and control of mulberry bacterial wilt, traditional chemical pesticides can be replaced, and environmental pollution is reduced.

The invention belongs to the technical field of microorganisms, and particularly relates to identification of a new species of Clostridium cell and application of the new species in high-efficiency synthesis of butyric acid by metabolizing acetic acid and ethanol. The Clostridium cell BJN0013 disclosed by the invention has the capability of metabolizing acetic acid and ethanol to produce butyric acid at high yield, and the yield can reach 31.11 + / -0.94 g / L, which is the highest yield of butyric acid synthesized by metabolism of known Clostridium microorganisms at present. According to the novel high-quality microorganism resource provided by the invention, an optimal microorganism resource is provided for functional microorganisms with high yield of flavor substances in the white spirit brewing process, and a strain foundation is laid for improving the flavor and quality of the white spirit.

The invention discloses a bacterial strain for broad-spectrum degradation of organic pollutants and application of the bacterial strain, the bacterial strain is classified and named as Acinetobacter venetianus, the bacterial strain number is GXGD-YZ-2508, the bacterial strain is preserved in Guangdong Microbial Culture Collection Center on August 27, 2025, the preservation number is GDMCC No: 66869, and the preservation address is the fifth floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City, Guangdong Province. The strain can grow under the condition that the salinity is 3wt%, can efficiently degrade 4-ethylphenol (4-EP), 2, 4-di-tert-butylphenol (2, 4-DTBP) and terephthalic acid (PTA), is suitable for biological treatment of aromatic pollutant-containing wastewater such as refining wastewater and PTA production wastewater, and has the advantages of high treatment efficiency, strong environmental adaptability and the like. The invention provides a novel microbial resource and an effective technical support for biological treatment of refractory aromatic pollutants, and has a wide application prospect.

The invention discloses pseudomonas chlorophenolate with a carmine degrading function and application of the pseudomonas chlorophenolate, and belongs to the technical field of microorganisms. The preservation number of the pseudomonas chlorophenol is GDMCC (China General Microbiological Culture Collection Center) NO: 65476. The viable bacteria and cell-free extracts of the strain can degrade carmine acid, and have wide application prospects. The strain has the characteristics of broad-spectrum pH adaptability and no harm to the environment, has the advantages of being low in cost, safe in component, natural, energy-saving, environment-friendly and the like when being used for industrially preparing a microbial preparation, can be used as a potential microbial resource in treatment of waste water containing carmine acid dye, and provides a new way for treating the waste water through a microbiological method.

The invention discloses a Burkholderia cepacia MZ-8 which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on April 8, 2025, and the preservation number of the Burkholderia cepacia MZ-8 is GDMCC No: 66106. The research finds that the burkholderia cepacia MZ-8 can efficiently inhibit the growth of pathogenic bacteria of citrus canker, the inhibition rate of the burkholderia cepacia MZ-8 to the pathogenic bacteria of citrus canker reaches 98.27% in a pot culture test, and meanwhile, the burkholderia cepacia MZ-8 can dissolve organic phosphorus, generate iron carriers and fix nitrogen and promote plant growth. Therefore, the burkholderia cepacia MZ-8 disclosed by the invention has good application potential in the aspects of preventing and treating citrus canker, promoting absorption and growth of phosphorus, iron and nitrogen of plants and the like. The discovery of the bacterial strain enriches beneficial microbial resources for biocontrol and growth promotion, and provides a bacterial strain which is stable in antibacterial effect, efficient and environment-friendly for green biological control.

The invention discloses a salt-tolerant strain for degrading 2, 4-di-tert-butylphenol, which is classified and named as Acinetobacter junii, has the strain number of GXGD-YZ-2506 (D1), is preserved in the Guangdong Microbial Culture Collection Center on July 4, 2025, has the preservation number of GDMCC No: 66641, and is preserved in the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City, Guangdong Province. The strain can grow under the condition that the salinity is 7wt%, can efficiently degrade 2, 4-di-tert-butylphenol, is suitable for treating high-salt organic wastewater containing 2, 4-di-tert-butylphenol in the industries of petrifaction, metallurgy, chemical engineering and the like, and has the advantages of being high in treatment efficiency, high in environmental adaptability and the like. The invention provides a novel microbial resource and an effective technical support for biological treatment of refractory phenol pollutants, and has a wide application prospect.

The invention relates to the technical field of environmental microbial resource development and sewage denitrification. The invention discloses an efficient denitrification strain Paracoccus sp.N21 with a flocculation characteristic, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.34710. The invention further discloses a preparation method of the efficient denitrification strain Paracoccus sp.N21. The strain obtained by the invention can completely remove ammonia nitrogen within 12 hours and thoroughly remove nitrate nitrogen within 48 hours, the removal rate reaches 100%, and ammonia nitrogen is preferentially degraded in a mixed nitrogen source. The method has the unique advantages that the flocculation rate in the nitrification process and the flocculation rate in the denitrification process exceed 93.9%, and the usage amount of a flocculating agent can be greatly reduced. When actual kitchen wastewater is treated, the ammonia nitrogen removal rate reaches 100%, and the removal rate reaches up to 7.51 mg / (L.h). The application of the strain can effectively reduce the volume of a reactor, reduce energy consumption and operation cost, provides a new solution for simplifying a biological denitrification process, is particularly suitable for treating high-ammonia-nitrogen kitchen wastewater, and has remarkable environmental and economic benefits.

The invention belongs to the technical field of biological prevention and control of plant diseases, and provides application of bacillus subtilis in prevention and control of verticillium wilt. The preservation number of the bacillus subtilis SEB-34 is CGMCC (China General Microbiological Culture Collection Center) No. 26419. The SEB-34 strain and the microbial agent produced by the SEB-34 strain can be used for preventing and treating plant diseases caused by verticillium dahliae, and the strain can be colonized at plant roots and keep high abundance and has the effects of preventing diseases, promoting growth and increasing yield. In the microbial agent provided by the invention, thalli are in a semi-dormant state, so that the microbial agent has relatively strong stress resistance and is more stable to store, and the production and transportation costs are effectively reduced while the effect is ensured. The invention also provides beneficial microbial resources for green prevention and control of economic crop diseases, and has good application prospects and high application values.

The application discloses an Aeromonas media strain capable of degrading PAEs and an application thereof, and belongs to the technical field of microorganisms. The strain is Aeromonas media HJ02, and the preservation number is GDMCC NO: 66828. The strain has obvious advantages in environmental adaptability and PAEs pollutant degradation activity, can efficiently degrade various PAEs and intermediate metabolites, and maintains good growth kinetic characteristics in a 0-120 h culture period. When the initial concentration of diisooctyl phthalate (DEHP) is 300 mg / L, the strain can significantly reduce the residual content of DEHP in the culture system after culture, and the 24 h degradation rate reaches 91.73 %. The application has the advantages of simple operation, safety and economy, provides efficient and stable microbial resources for PAEs pollution remediation, and has important practical value.

The invention provides application of platanus sp. Su100 in promotion of synthesis of poplar melatonin and disease resistance, and relates to the technical field of microbial resource development and forest cultivation. The soft disc fungus Su100 can activate the activity of a key enzyme for synthesizing melatonin in a poplar body, and the content of the melatonin is remarkably increased. As a plant endogenous antioxidant and a multifunctional signal molecule, the melatonin plays a core role in plant stress resistance, growth regulation and control, quality improvement and ecological restoration. The floppy disc fungi have high colonization efficiency in plants, and can form a symbiotic system with the poplar by utilizing a floppy disc fungi inoculation technology to play a role for a long time. The application of the invention can significantly reduce the production cost of agriculture and forestry, is especially suitable for crop cultivation under the adverse conditions of drought, salt and alkali, diseases and the like, reduces the application of chemical fertilizers and pesticides, and lays a foundation for the construction of an efficient, green and sustainable forestry planting and cultivation system.

The present application belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating Guaiacum officinale anthracnose, a fermentation liquor, a biocontrol bacterium agent and application. Bacillus velezensis A biocontrol bacterium is isolated and screened from a microbial resource on a healthy Guaiacum officinale leaf, and is classified and named as Bacillus velezensis , which is preserved in the China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided in the present application has good antagonistic effects on Colletotrichum gloeosporioides causing Guaiacum officinale anthracnose, on Stemphylium botryosum causing Guaiacum officinale leaf spot disease, on Botryosphaeria dothidea, on Alternaria alternata and on Pestalotiopsis versicolor capable of causing plant diseases. The biocontrol bacterium, the fermentation liquor and the biocontrol bacterium agent provided in the present application have potential application prospects in preventing and treating Guaiacum officinale anthracnose.

The invention discloses bacillus subtilis 12-10-2 and application thereof in synergistic preparation of fish oil and fish sauce, and belongs to the technical field of microbial fermentation. The preservation number of the bacillus subtilis (Bacillus subtilis) 12-10-2 is CGMCC (China General Microbiological Culture Collection Center) No.34966. The bacillus subtilis (Bacillus subtilis) 12-10-2 can be used for preparing the bacillus subtilis. The bacillus subtilis 12-10-2 can be used for efficiently hydrolyzing protein in a high-salt environment. The bacillus subtilis 12-10-2 is used for performing bacterium-adding fermentation on the snakehead processing leftovers, so that the fish oil and the fish sauce can be synergistically prepared, and the obtained product is high in yield and good in quality. A new microbial resource is provided for high-value utilization of snakehead processing leftovers, the problems of environmental pollution and resource waste caused by improper treatment can be solved, a new technical support is provided for establishment of a preparation process of high-quality fish oil and fish sauce, and the method has a wide market application prospect.