136 results about "Acid-fast" patented technology

The invention discloses an enteric-solubility multilayer encysted bacterium microcapsule and making method in the biological agent technical domain, which is characterized by the following: adopting sodium alginate, calcium chloride and chitose as clad material of microcapsule; making lactic acid bacteria or bifidobacteria as core-clad material; proceeding ionic exchange for sodium alginate and calcium chloride; forming second clad on the surface of calcium alginate through different isoelectric points of calcium alginate and chitose; freezing at low temperature to obtain the product.

The present invention demonstrated the potential use of Lactobacillus johnsonii D115 as a probiotic, as a prophylactic agent or as a surface treatment of materials against human and animal pathogens such as Brachyspira pilosicoli, Brachyspira hyodysenteriae, Shigella sonnei, Vibrio cholera, Vibrio parahaemolyticus, Campylobacter jejuni, Streptococcus pneumoniae, Enterococcus faecalis, Enterococcus faecium, Clostridium perfringens, Yersinia enterocolitica, Escherichia coli, Klebbsiella pneumoniae, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Aspergillus niger and Fusarium chlamydosporum. The proteineous antimicrobial compound was partially characterized and found to be heat tolerant up to 121° C. for 15 min, and acid tolerant up to pH1 for 30 min at 40° C. The compound is also stable to enzymatic digestion, being able to retain more than 60% antimicrobial activity when treated with pepsin and trypsin.

This invention relates to new oxazolidinones having a cyclopropyl moiety, which are effective against aerobic and anerobic pathogens such as multi-resistant staphylococci, streptococci and enterococci, Bacteroides spp., Clostridia spp. species, as well as acid-fast organisms such as Mycobacterium tuberculosis and other mycobacterial species.

The compounds are represented by structural formula I:

its enantiomer, diastereomer, or pharmaceutically acceptable salt or ester thereof.

The invention encompasses hexapeptides consisting of alternating hydrophobic residues (B) at positions 2, 4, and 6, hydrophilic, hydrophilic, charged residues (X) at positions 1 and 3, and a naphthylalanine (Nal), an aliphatic or aromatic residue (O) at position five, represented generally by the formula XBXBOB, which exhibit antimicrobial activity against infections caused by a variety of pathogens. These pathogens may include gram positive or negative bacteria, acid-fast bacteria such a mycobacteria, parasites, dermatophytes, or fungal pathogens. Typical fungal pathogens include Candida albicans and typical dermatophytes include Trichophyton rubrum and Trichophyton mentagrophytes. The hexapeptides of the present invention exhibit antifungal activity, antibacterial activity, desirable stability, and lack toxicity to the mammal receiving treatment.

The present invention relates to certain substituted phenyl piperazinyl oxazolidinones, for example, to those having the structure of Formula I

with the variables as defined within, and to processes for the synthesis of the same. This invention also relates to pharmaceutical compositions containing the compounds of the present invention as antimicrobials. The compounds are useful antimicrobial agents, effective against a number of human and veterinary pathogens, including gram-positive aerobic bacteria such as multiply-resistant staphylococci, streptococci and enterococci as well as anaerobic organisms such as Bacterioides spp. and Clostridia spp. species, and acid fast organisms such as Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium spp.

The present invention relates a novel Lactobacillus sp. Strain and usc thereof. More specifically, the present invention relates to a novel Lactobacillus sp. Strain, Lactobacillus paracasei, isolated and identified from Kimchi fermentation liquid and uses thereof as probiotics, feeding additive, deodorizing agent and food additive. Lactobacillus paracasei of the present invention has good acid-resistance, bile acid-resistance and stability in the human body, has pathogenic bacteria inhibiting activity, increases numbers of beneficial bacteria by changing intestinal microflora and decreases diarrhea incidence, deodorizes offensive odor, and has effectes of softening and deodorizing in meat and fish.

The present invention discloses a method for assembling foot-and-mouth disease virus empty capsids in insect cells via the alteration of acid-resistance. The method for assembling foot-and-mouth disease virus empty capsids in insect cells includes the following steps: (1) the altered P12A gene and the non-structural protein gene 3C of foot-and-mouth disease virus are introduced into bacteria via baculovirus vectors for recombination to produce recombinant rhabdovirus A; (2) the DNA of the recombinant rhabdovirus A is used to transfect the insect cells, so that the foot-and-mouth disease virus empty capsids are obtained. The method assembles the integral foot-and-mouth disease virus empty capsids in the insect cells for the first time, lays a foundation for the research and the development of gene-engineered subunit vaccines and novel diagnostic reagents.

Bacilliis natto NLSSc with the preservation number CGMCC No. 0724 is Gram staining positive, non-acid fast, straight or near straight, (0.6-0.8)x(1.5-2.0) micron sized and aerobic; has mesial and un-swelled spore with the spore number inside one sporange cell not more than one, meso diamino heptane diacid and glycin contained in the cell wall, no characteristic saccharum and peripheral flagellum; and can be grown well in five kinds of natural organic culture medium and can form relatively thick mycoderm and butter fat colored colony with smooth edge in soybean cake powder culture medium. Bacilliis natto can be cultured in culture medium with nitrogen source to obtain thrombus dissolving matter natto kinase and the cultured matter may be extracted to obtain powdered matter as the additive for thrombolytic health food.

This invention relates to new oxazolidinones having a cyclopropyl moiety, which are effective against aerobic and anerobic pathogens such as multi-resistant staphylococci, streptococci and enterococci, Bacteroides spp., Clostridia spp. species, as well as acid-fast organisms such as Mycobacterium tuberculosis and other mycobacterial species.

The compounds are represented by structural formula I:

its enantiomer, diastereomer, or pharmaceutically acceptable salt or ester thereof.

The invention provides bacillus licheniformis, wherein the classification name is bacillus licheniformis CY-1, the bacillus licheniformis CY-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address is the Institute of Microbiology of Chinese Academy of Sciences located in 3, Courtyard 1, West Beichen Road, Chaoyang District, Beijing, the postal code is 100101, the accession number is CGMCC NO.12448, and the preservation date is May 13, 2016. The invention further provides applications of bacillus licheniformis in kitchen waste treatment. The bacillus licheniformis has the activities of high-activity protease, amylase and oil hydrolase, can degrade glucose for generating acid, and is a thermophilic bacterium with high salt tolerance and high acid tolerance, and through effective combination of the bacillus licheniformis with a carrier, the weight of the fed kitchen waste is rapidly and efficiently reduced by 85%.

The invention relates to the biotechnical field, particularly relates to the field of production and development of functional lactic acid bacteria, more particularly relates to exopolysaccharides-producing lactobacillus plantarum for adsorbing monophthalate effectively. The strain number of the exopolysaccharides-producing lactobacillus plantarum for adsorbing monophthalate effectively is RS20D, and the exopolysaccharides-producing lactobacillus plantarum for adsorbing monophthalate effectively is registered and preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.13272 and the preservation date of November 14, 2016. The lactobacillus plantarum RS20D provided by the invention is functional lactic acid bacteria which has acid resistance, grows well in an environmental condition of pH 3.0-9.0, and particularly has the characteristic of adsorbing monophthalate and producing exopolysaccharides, for example, the lactobacillus plantarum RS20D is developed to a health-care food or drug which is taken for a long time to improve intestinal flora, and the damage of a phthalate plasticizer on a human body is reduced; or, the lactobacillus plantarum RS20D is input to a fermenting diary product or a cheese product, so that the sensory characteristic of the product can be improved.

The invention discloses a cysteine quick detection method based on an absorption spectrum of Au nanoparticle collosol. The invention is characterized in that deionized water, buffer solution with 3.8 to 5.7 pH value and Au nanoparticle collosol stock solution are mixed to be prepared into detection solution, and the concentration of the buffer substance and the element Au in the detection solution is respectively 10 plus or minus 1mm and 0.9 plus or minus 0.1mm; the cysteine-containing liquid to be detected is added to the detection solution, the concentration of the cysteine in the formed detection system is 0.5 to 10mum, and the absorption spectrum is measured within ten minutes after even mixing; and according to the difference of the specific value eta of the absorbance of 700 nanometers and 525 nanometers of the wavelength in the absorption spectrum of the detection system, the concentration of the cysteine can be detected quantitatively. The method greatly reduces the time required for detecting the cysteine, and meanwhile, has the advantages of simple equipment, simple operation and mass detection.

The invention provides bacillus cereus and an application of the bacillus cereus as an agricultural biological agent and relates to the bacillus cereus and the application of the bacillus cereus in the agricultural field. The bacillus cereus solves the problem that microorganism engineering strains for degrading organophosphorus pesticide are single in function in the prior art. The bacillus cereus WSW-P05 is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the bacillus cereus WSW-P05 is CGMCC No: 10605. The bacillus cereus WSW-P05 serves as the agricultural biological agent. The bacillus cereus WSW-P05 serves as the agricultural biological agent so that crop growth can be promoted, and the crop yield can be increased; the organophosphorus pesticide in crops and soil is degraded; acid resistance is achieved; multiple pathogenic bacteria can be restrained; multiple functions are achieved.

The invention discloses an acid-resistant pseudomonas koreensis strain CLP-7 which is capable of preventing diseases and promoting growth, and can be used for biocontrol. The acid-resistant pseudomonas koreensis strain CLP-7 is preserved at China General Microbiological Culture Collection Center, on 27th, October, 2016, and the preservation number is CGMCC No.13204. The antibacterial spectrum of the acid-resistant pseudomonas koreensis strain CLP-7 is relatively large; the acid-resistant pseudomonas koreensis strain CLP-7 is capable of inhibiting growth of Phytophthora parasitica var. nicotianae, Ralstonia solanacearum, and Alternaria alternata Keissler; the antagonistic activity under acidic conditions is high, the acid-resistant pseudomonas koreensis strain CLP-7 is capable of producing siderophore, possesses protease and glucanase activity, and potassium releasing capacity. The results of biocontrol pot experiment show that the acid-resistant pseudomonas koreensis strain CLP-7 is capable of preventing under acidic soil conditions, and promoting growth and chlorophyll synthesis of tobacco seedlings in acidic soil, so that the acid-resistant pseudomonas koreensis strain CLP-7 and microorganism bacterium agents of the acid-resistant pseudomonas koreensis strain CLP-7 can be used for preventing tobacco fungi and bacterial root and stem diseases under continuous cropping or acidic soil conditions effectively, and is high in application value.

The invention discloses genes and engineering bacteria of acid and high temperature resistant alpha-amylase and a preparation method of the genes and engineering bacteria. Compared with a mutant reported by literature, under the condition that the pH ranges from 4.2 to 4.6, and the temperature is 95 DEG C, the thermostability of the acid and high temperature resistant alpha-amylase is enhanced, the expression quantity is 50 times that of existing acid and high temperature resistant alpha-amylase, the alpha-amylase is applied to the fields of chemical industry, food, medicine and the like, starch can be efficiently degraded under the conditions of strong acidity and high temperature, the process can be simplified, environmental pollution is reduced, and a wide application prospect is achieved. Meanwhile, according to genes and engineering bacteria of the acid and high temperature resistant alpha-amylase and the preparation method of the genes and engineering bacteria, rational design of applied combined construction prediction has important guiding significance in improving thermostability of an industrial enzyme.

The invention provides acid-resistant pseudomonas protegens CLP-6 and application thereof. According to the acid-resistant pseudomonas protegens CLP-6 and the application thereof, the bacterial strainof the pseudomonas protegens CLP-6 is preserved in China General Microbiological Culture Collection Center on October 27th, 2016, and the biological preservation number of the pseudomonas protegens CLP-6 is CGMCC No.13205; the bacterial strain of the pseudomonas protegens CLP-6 has acid resistance, is good in disease prevention and plant growth promotion effects and high in stress resistance, hasthe antagonistic activity of pathogenic bacteria, is wide in soil acidity tolerance range and wide in antibacterial spectrum, and has application and development potential in prevention and treatmentof bacterial wilt, black shanks and brown spots; and meanwhile, under the general background that the acidification conditions in China is increasingly severe, the research on the bacterial strain ofthe pseudomonas protegens CLP-6 has reference significance in prevention and treatment of plant diseases under various soil qualities, a microbial resource is provided for green prevention and control over the bacterial wilt, the black shanks and the brown spots in acid soil, and secondary metabolites of microorganisms are excavated, so that an important foundation can be laid for the biocontrolefficacy on the bacterial wilt, the black shanks and the brown spots in the acid soil.

The invention belongs to the field of biomedical detection, relates to the technical field of microbe molecule biology detection, in particular to a multiplex polymerase chain reaction (PCR) kit capable of quickly identifying and diagnosing mycobacterium tuberculosis, nontuberculous mycobacteria, mycobacterium bovis and Bacillus Calmette Guerin vaccine simultaneously, and discloses four primer pair sequences designed for specific segments of four differential genes, namely 16S rRNA, IS6110, Rv2652 and Rv3873 of the mycobacterium, and amplification conditions for multiplex PCR. By the invention, a mycobacteria tuberculosis complex sequence can be quickly and accurately amplified in a sputum sample of a tuberculosis patient, and species identification can be carried out; the sensitivity is 50 colony forming units/template, which is far higher than that of an acid-fast staining method, an inspection procedure for the mycobacterium tuberculosis is greatly simplified, and a detection speed is improved; and meanwhile, different mycobacterium strains can be identified through once PCR, and a technical means is provided for clinic quick diagnosis of tuberculosis and molecular epidemiology survey.

The invention discloses a Paecilomyces thermophila mutant strain and a mutation induction method and application thereof. The mutation induction method includes: Paecilomyces thermophila screened from high-temperature-fermented compost and used for producing high-temperature-resistant and acid-resistant glucose oxidase is used as the original strain, protoplast ultraviolet and ethyl methane sulfonate compound mutation induction is performed, and the screened strain is subjected to budding spore diethyl sulfate-microwave compound mutation induction to obtain the Paecilomyces thermophila mutant strain. The Paecilomyces thermophila mutant strain is preserved in China General Microbiological Culture Collection Center on January 11th, 2016, and the preservation number of the mutant strain is CGMCC No. 13182. Compared with a conventional mutation induction method, the mutation induction method has the advantages that the method is high in efficiency and simple in fast in screening, the productivity of the mutant strain is high, the yield of the mutant stain is 277.65% of that of the Paecilomyces thermophila original strain, fermentation liquor enzyme activity reaches 185U/ml, production cost is lowered, and the glucose oxidase produced by the mutant strain is good in high-temperature resistance and acid resistance and can be used as feed enzyme applied to feed industry.

The invention discloses a method for microencapsulating cholesterol-degrading lactic acid bacteria, which comprises the steps of the activation of the cholesterol-degrading lactic acid bacteria, the preparation of immobilized cholesterol-degrading lactic acid bacteria, the preparation of microcapsules by a W/O/W multiphase emulsion method, and freeze-drying. The cholesterol-degrading lactic acid bacteria microcapsules prepared by adopting the multiphase emulsion method (W/O/W) have the characteristics of stability, non-toxicity and the like due to the fact that wall materials thereof all belong to natural polymer materials; the release rate of the microcapsules in intestinal juice within 2h is over 87.4 percent, and the viable count can reach 7.03*109cfu/mL; the viable count can still reach 6.81*106cfu/mL after the microcapsules are placed for 3h at the temperature of 50 DEG C, and can still reach 6*108cfu/mL after the microcapsules are stored for 30d; in vitro cholesterol degradation tests, the degradation rate of cholesterol is over 36.07 percent; and the microcapsules have better acid resistance, enteric property, high temperature resistance and cholesterol degradation, and the yield is about 86.62 percent. Capsule materials and carrier materials have low cost, so the cost is reduced.