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Enteric-coated multilayer encapsulated probiotic microcapsule and preparation method thereof

A technology of microcapsules and probiotics, applied in the field of microbial preparations, can solve problems such as the influence of bacterial activity, difficulty in long-term storage, complicated coating process, etc., and achieve the effects of promoting absorption, prolonging storage time, and short exposure time

Inactive Publication Date: 2007-05-30
JINAN SYNBIOTICS BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: the coating process is complicated, and the operation time is long; during the operation, the probiotics are in contact with the air for a long time or the temperature is too high or changes too fast during the operation, causing the bacteria to be easily inactivated; and some The coating material has a certain influence on the activity of the bacteria, so it is not easy to store for a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Sodium alginate is made into 100ml of 2% solution, 100ml of 4% calcium chloride solution is prepared, and 100ml of 1%wt chitosan solution is prepared.

[0021] (2) Centrifuge 500 ml of lactic acid bacteria fermentation liquid to collect wet cells, and suspend the wet cells in 10 ml of physiological saline.

[0022] (3) Add the suspension of wet cells into the above-mentioned sodium alginate solution and mix evenly to obtain a suspension of sodium alginate cells.

[0023] (4) The sodium alginate cell suspension is made into micro-droplets, which are added into the above-mentioned calcium chloride solution and stirred to obtain calcium alginate microcapsule particles.

[0024] (5) The microcapsule particles were collected by centrifugation, and added to the above 1%wt chitosan solution for secondary coating.

[0025] (6) The multi-layer coated microcapsule particles were collected by centrifugation and freeze-dried.

[0026] (7) The diameter of the microcapsule part...

Embodiment 2

[0034] Centrifuge 500ml of bifidobacterium fermentation broth to collect wet bacteria, and dilute to 10ml with phosphate buffer solution with a pH value of 6.8. The obtained wet cell suspension was added to 100 ml of 2% sodium alginate solution and mixed evenly to obtain a sodium alginate cell suspension. The sodium alginate cell suspension is made into micro-droplets, which are added into 100 ml of 3% calcium chloride solution and stirred to obtain microcapsule particles coated with calcium alginate.

[0035] The microcapsule particles were collected by centrifugation, and then added to 100 ml of 2% chitosan solution for secondary coating.

[0036] The above-mentioned microcapsule particles are collected by centrifugation and freeze-dried. The diameter of the dried microcapsule particles is 10-100 μm. Enteric multi-layer coated probiotic microcapsules were obtained.

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Abstract

The invention discloses an enteric-solubility multilayer encysted bacterium microcapsule and making method in the biological agent technical domain, which is characterized by the following: adopting sodium alginate, calcium chloride and chitose as clad material of microcapsule; making lactic acid bacteria or bifidobacteria as core-clad material; proceeding ionic exchange for sodium alginate and calcium chloride; forming second clad on the surface of calcium alginate through different isoelectric points of calcium alginate and chitose; freezing at low temperature to obtain the product.

Description

1. Technical field [0001] The invention relates to a microbial preparation, in particular to an enteric multi-layer coated probiotic microcapsule and a preparation method thereof. 2. Background technology [0002] As a natural biologically active preparation, microbial preparations are non-toxic, non-drug resistant, and non-residual, and have the effects of enhancing immunity, promoting growth, and improving feed utilization. They have been widely used in human health food and can be used as feed additives Used in animal husbandry and aquatic animal breeding. See Chinese patent application 200510045358.7 published on July 12, 2006, "multifunctional probiotic preparation and its preparation method". [0003] Probiotic preparations are mainly live bacteria preparations, most of which are obligate anaerobic bacteria. Among them, lactic acid bacteria and bifidobacteria are a type of anaerobic bacteria, which are difficult to survive in the air, dry or at room temperature, short...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61K9/50A61K47/36A61P3/02A23L1/30A23K1/16A23K20/00A23L33/10A61K35/741
Inventor 王荻郝永仁楚杰张大伟
Owner JINAN SYNBIOTICS BIOENG
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