748 results about "Aquatic animal" patented technology

The invention provides a molecular marker related to the salinity tolerance character of grass carp, an amplification primer of the molecular marker and application of the molecular marker in salt-tolerant breeding of the grass carp, and belongs to the technical field of breeding of aquatic animals. The molecular marker related to the salinity tolerance character of the grass carp is obtained by screening through whole genome association analysis (GWAS), and T / C polymorphism exists at the 25391590 site of a chromosome 3 or A / G polymorphism exists at the 9605803 site of a chromosome 10. The molecular marker is remarkably related to the salt tolerance character of the grass carp, two pairs of amplification primers are developed for the molecular marker, selective breeding of the grass carp can be assisted, salinity tolerance parents of the grass carp can be efficiently identified, and the salt tolerance breeding progress of the grass carp is accelerated.

The invention discloses a photodynamic inactivation parameter adjusting method based on hybrid fuzzy-PID control, relates to the technical field of parameter fuzzy control, and aims at the problem that photodynamic inactivation efficiency and aquatic organism safety in aquaculture are difficult to consider at the same time through multi-sensor fusion and environment prediction. Then, a fuzzy-PID self-adaptive putting technology is adopted to be coupled with an illumination strategy, and accurate matching of the photosensitizer dosage and the light intensity is achieved; water body fluctuation and pathogen diffusion are dealt with through real-time closed-loop and multi-scene collaborative scheduling; multi-dimensional index evaluation is utilized, reinforcement learning is introduced to carry out self-learning optimization, and putting and illumination parameters are continuously improved; and finally, the method is expanded to large-scale distributed control and cloud big data training, and efficient inactivation and flexible deployment can be kept in different breeding scenes. Pathogens can be effectively prevented from being rapidly diffused in an adverse environment, damage to aquatic animals caused by excessive accumulation of reactive oxygen species (ROS) is reduced, and meanwhile energy consumption and resource waste are reduced.

The invention belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding, and particularly discloses an SNP (Single Nucleotide Polymorphism) molecular marker remarkably related to grass carp body weight traits and application thereof. The SNP molecular marker is shown as follows: (1) SLG1123629389 with the polymorphic base being T / C, (2) SLG1127776299 with the polymorphic base being A / G, (3) SLG1127776356 with the polymorphic base being G / A, and (4) SLG1235362696 with the polymorphic base being T / C. The invention also discloses application of the SNP molecular marker, the amplification primer or the kit in breeding grass carp with excellent growth traits. The SNP molecular marker disclosed by the invention can be used for early breeding of grass carp with excellent growth traits, so that the breeding workload is greatly reduced, the breeding time can be remarkably shortened, the breeding process is accelerated, the breeding efficiency is improved, and the breeding cost is reduced; the method has important guiding significance for improving the production performance of the grass carp, reducing the culture cost of the grass carp and increasing the culture income, and is suitable for popularization and application.

The invention discloses a method for obtaining high-quality fertilized eggs of wild sal-family pseudosciaena crocea, and belongs to the technical field of aquatic animal breeding. The method comprises the following steps: capturing wild parent fishes in the breeding season of the sal-family pseudosciaena crocea and transporting the wild parent fishes to a farm; the method comprises the following steps: carrying out temporary culture and intensive culture on wild parent fishes, selecting male and female wild parent fishes with mature gonads, anesthetizing, and injecting artificial spawning induction mixed hormones; after artificial spawning mixed hormones are injected, ova and seminal fluid are collected, and high-quality fertilized ova are obtained through artificial insemination. According to the method, all the technical steps are associated with one another to form a whole, the high-quality fertilized eggs of the large yellow croakers of the wild sal ammoniac family can be successfully obtained, and the proportion of the obtained high-quality fertilized eggs reaches 45% or above. According to the method, the obtained high-quality fertilized eggs are adopted for fry hatching, the hatching rate of the obtained fertilized eggs reaches 62% or above, the fertilized eggs can be used for constructing and breeding a wild sal-family pseudosciaena crocea basic population, and artificial breeding of the sal-family pseudosciaena crocea is achieved.

The invention provides an intelligent control system and method for aquaculture. The intelligent control system comprises a dissolved oxygen detector, a pH detector, an ammonia nitrogen detector, a redox potentiometer, a structured data front-end processor, an underwater binocular vision observation device, a camera, an unstructured data front-end processor, a cloud processor and front-end equipment. The cloud processor makes a decision according to the information of the structured data front-end processor and the unstructured data front-end processor, and controls the front-end equipment to adjust the operation of the culture pond. According to the aquaculture monitoring system, aquaculture water quality key parameters such as dissolved oxygen, pH value, ammonia nitrogen and oxidation-reduction potential are monitored, aquatic animals are monitored in real time in the aquaculture process, the aquaculture environment is dynamically regulated and controlled, and the intelligence of aquaculture is improved. The intelligent control system for aquaculture can be used as a minimum unit template, is efficient and stable in operation, and facilitates the realization of whole-course real-time monitoring, autonomous regulation and control, multi-link coordinated linkage control and intelligent decision-making of aquaculture through copying and popularization.

The invention belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker remarkably related to growth traits of grass carp and application of the SNP molecular marker. The SNP molecular marker is located at the position of 23608308bp of the eleventh chromosome (SLG1123608308) of the grass carp, and is Tgt; and A basic group mutation. Experiments show that the SNP molecular marker developed by the invention has significant influence on four growth traits (Plt; 0.05), and the allele A has more excellent growth traits. The invention discloses application of an SNP (Single Nucleotide Polymorphism) molecular marker, an amplification primer or a kit in breeding grass carp with excellent growth traits. The SNP molecular marker provided by the invention is not limited by the age, sex and the like of the grass carp, can be used for breeding grass carp varieties with excellent growth traits, and even can be accurately screened after being hatched, so that the breeding process of the grass carp varieties with excellent growth traits is greatly accelerated.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of litopenaeus vannamei and application of the SNP molecular marker, and belongs to the field of molecular marker-assisted breeding of aquatic animals. The genome DNA of the muscular tissue of the litopenaeus vannamei to be detected is extracted as a template, the gene is subjected to PCR amplification and sequencing, the genotype of the molecular marker SNP1-3 is determined, and the molecular marker SNP1-3 is used as the SNP molecular marker for seed selection, so that the aim of quickly screening litopenaeus vannamei individuals with obvious growth advantages in the early stage is fulfilled. According to the molecular marker related to the growth traits of the litopenaeus vannamei, the breeding period can be greatly shortened, the fine breed breeding process of the litopenaeus vannamei is accelerated, the breeding efficiency is improved, and the breeding cost is reduced.

The invention relates to the technical field of data processing, and discloses an aquaculture intelligent monitoring method and system based on the Internet of Things. The method comprises the following steps: collecting pond environment parameters and acoustic signals through layered arrangement of sensors, and obtaining multi-pond linkage data through wireless transmission; performing Fourier transform processing on the data to generate biology-environment coupling data; the water quality correlation degree between the ponds is calculated, and regional balance control data are formed; biological activity rules are analyzed, and biological rhythm feeding data are obtained; checking the carbon footprint to generate low-carbon operation data; and performing multi-dimensional correlation analysis to form ecological breeding optimization data. According to the method, the layered and partitioned ecological sensing network is constructed, collaborative analysis is performed on multi-pond environment data, and intelligent management of the breeding process is realized in combination with biological rhythm characteristics and carbon emission control requirements of aquatic animals, so that the breeding efficiency is improved, and the carbon emission intensity is reduced.

The invention belongs to the technical field of micro-ecology, and particularly relates to an aquaculture compound micro-ecological preparation and application thereof. The compound microecological preparation for aquaculture comprises bacillus silage YZS-M01, bacillus pettimensis YZS-M02, bacillus velezensis YZS-M03 and bacillus siamensis YZS-M04, and the total viable concentration of the compound microecological preparation is (2-5) * 10 < 9 > cfu / ml or (2-5) * 10 < 9 > cfu / g. The compound micro-ecological preparation for aquaculture has a remarkable purification effect on aquaculture water, and meanwhile, the survival rate and the yield of aquatic animals can be increased.

The invention relates to the technical field of aquaculture, and discloses an ecological aquaculture system, which comprises a culture pond as a basic component of the whole device and used for bearing and assembling a central data processing system and a subordinate lower structural component thereof; the central data processing system is used for receiving, processing and storing data from each monitoring module; the multi-parameter water quality monitoring module monitors key water quality indexes including dissolved oxygen, temperature, pH value, ammonia nitrogen content and nitrite content in real time; the aquatic animal growth monitoring module is used for measuring growth parameters including body length and body weight of aquatic animals through an image recognition technology; through the multi-parameter water quality monitoring device, the culture environment and the state of the aquatic animals can be mastered in real time, so that accurate management is performed, and the disease early warning device can find disease risks in an early stage, take prevention and treatment measures in time and reduce the influence of diseases on the growth of the aquatic animals.

A Method for building a digital twin for a living system, whereas such a system is an organism, preferably a plant, an algae, a water-borne animal, a fungus, and / or a worm, wherein A Provision of a number of multiple living systems of the same type; B Growing the living systems of the same type under different growing conditions; C Determination of one or more parameters of the living system that is or are subjected to a change in over the time period of the growth; wherein multiple data sets are determined and stored, each data set has said parameter or parameters and a time stamp of the time of determination; and D Generation of a digital twin for said living system based on said multiple data set representing different growth stages of said living system.

The invention discloses a nano-carrier for gene editing of aquatic animals. The nano-carrier comprises a) TNP nano-particles and b) Cas9 mRNA and sgRNA. The invention also discloses an application of the TNP nano-particle as an mRNA delivery carrier and a gene editing method based on the nano-carrier, according to the method, the TNP nano-particle with good biocompatibility is used for packaging Cas9 mRNA and sgRNA, and an efficient and low-damage gene editing delivery mode is realized. The nano-carrier is delivered into an embryo of an aquatic animal in a non-invasive manner, so that the transfection efficiency and the specificity of gene editing are optimized. In addition, the method can improve the gene editing efficiency and precision and enhance the embryo survival rate, is simple, convenient and safe to operate and wide in applicability, can support customized gene editing, and is beneficial to promoting the application of gene editing in aquatic product germplasm genetic improvement.

The invention discloses a low-carbon freshwater fish feed added with fermented bamboo powder and application of the low-carbon freshwater fish feed, and belongs to the technical field of aquatic animal feeds. The low-carbon freshwater fish feed capable of reducing the fat rate of fish bodies and / or reducing nitrogen and phosphorus emission is prepared from the fermented bamboo powder, and the content of vitamins, enzymes and growth factors in the feed can be increased by adding the fermented bamboo powder, so that the freshwater fish bodies have good anti-pathogenic activity; the absorption and utilization of the freshwater fish on nutritional ingredients and fiber-rich ingredients in the feed are improved, so that the aim of reducing the emission of nitrogen and phosphorus is fulfilled; meanwhile, the fermented bamboo powder is used for partially replacing soybean meal in the freshwater fish feed, so that the dosage of feed raw materials such as the soybean meal is reduced, the intestinal health of the freshwater fish is improved, the muscle quality of the freshwater fish is improved, the earthy smell in muscles is reduced, the body type of the freshwater fish is also improved, and the fat content in abdominal muscles is reduced.

The invention relates to systems, apparatus, and methods of objects such as aquatic animals (e.g. fish) in a harvesting environment. There may be provided A system for monitoring aquatic animals (e.g. fish) at harvest (e.g. in real time) comprising: a (e.g. electric) stun station; a dewatering station; optionally, a processing station (e.g. a post-stun processing station to elicit death, such as percussive stun and / or bleed, and / or ice treatment, and so on); at least one imaging station; and, at the at least one imaging station, an image acquisition unit comprising: at least one camera module configured for capturing images of aquatic animals post (e.g. electric) stun as these pass through the imaging station; an image processing unit of, or operably coupled to, the image acquisition unit comprising: at least one microprocessor; at least one storage medium comprising one or more programs, the one or more programs configured for execution on the at least one microprocessor, the one or more programs comprising instructions for: detecting at least one object in at least one image frame; determining at least one condition associated with the object in the at least one image frame; the at least one condition comprising a first condition of the object being determined to be an aquatic animal of interest; and, in response to the determination of the at least one condition, taking an action associated with the harvesting of aquatic animals.

The invention belongs to the technical field of aquatic animal zebra fish breeding water environment purification, and particularly relates to an aquatic animal zebra fish breeding system which comprises a frame body, a water supply port and a water tank, a sewage discharging groove, a tank base and a water adding valve are connected to the frame body, a sewage discharging pipe is connected to the sewage discharging groove, a tank body is installed on the tank base, and a water adding port is formed in a cover plate of the tank body. The inside of the tank body is divided into a sewage discharge chamber and a feeding chamber through a filter screen, a sewage discharge hole penetrates through the bottom of the filter screen, a siphon is installed between the sewage discharge chamber and a sewage discharge groove, a siphon hole is formed in the siphon, the water supply port is communicated with the water tank, and a main water inlet valve is connected to a communication pipeline. The sewage chamber is communicated with the water storage chamber through a sewage filter, the sewage chamber is further communicated with a blow-off pipe, the water storage chamber is communicated with a water adding valve, and a circulating water pump is connected to a communicating pipeline. Particles in the feeding room can be automatically discharged in real time, regular maintenance and replacement of the filter element are not needed, manpower is saved, and material consumption is avoided.

The invention discloses a machine learning-based Bellamya piriformis gender prediction method and application, and belongs to the technical field of aquatic animal gender determination. According to the method, shell shape character data of the Bellamya piriformis is taken as a training sample, and a multilayer perceptron model (MLP), a principal component analysis dimensionality reduction model (PCA) and a random forest model are respectively constructed to extract gender difference characteristics of the Bellamya piriformis. The performance and prediction accuracy of the constructed MLP model are optimal, the prediction accuracy reaches 99.1%, and the recall rate and the F1 score both exceed 95%. The method breaks through the limitation of the existing bellamya piriformis sex identification method, can quickly and accurately predict the sex of the bellamya piriformis, and provides convenience for breeding of new varieties of the bellamya piriformis and screening of male and female parents in artificial propagation.

The invention discloses an ecological restoration system for mangrove forest wetlands, and relates to the technical field of ecological restoration of coastal wetlands, and the technical key points are as follows: the ecological restoration system comprises an algae-cleaning biocenosis: the number of algae is reduced through salt-tolerant algae-cleaning organisms, and the transparency of a water body is improved; the sea grass plant community comprises Sichuan fructus viticis and halophila acuminata, absorbs eutrophic substances in water through photosynthesis, and further purifies the water quality; aquatic animals comprise fishes, benthonic animals, shrimps and filter-feeding zooplanktons; the mangrove plant community is used for planting bruguiera gymnorrhiza, kandelia candel and aegiceras corniculatum in a layered manner according to tide and water depth so as to realize the functions of purifying water quality of an ecological system and providing a habitat; and the environment monitoring module is provided with tide monitoring equipment, observes tide water level change and salinity and alkalinity, and implements early warning and emergency measures under extreme climate conditions. According to the method, a multi-layer producer community system can be coupled and repaired, the eutrophication problem can be effectively solved by multi-dimensionally improving the biodiversity, and a new method is provided for coastal wetland repair.

The invention provides a biological fermentation feed for enhancing immunity of aquatic animals and a preparation method of the biological fermentation feed, and relates to the technical field of aquaculture. The biological fermentation feed is prepared by compounding fish bone meal, a rice bran fermentation material, soybean meal, a compound fermentation material, visceral meal, orange peel powder, corn starch, compound vitamins, tea saponin and protocatechuic acid. According to the present invention, the defects in the prior art are overcome, the weight gain growth of the aquatic product is comprehensively ensured through the compound addition of the microbial components of the aquatic product feed and the regulation and control of other components, the immunity of the aquatic product is further improved, and the artificial breeding benefit of the aquatic product is comprehensively ensured.

The invention belongs to the technical field of disease prevention and treatment and aquaculture, and relates to application of acacetin in preparation of a medicine for preventing and treating white spot syndrome. The invention discloses that the acacetin has the biological activity of resisting the white spot syndrome virus for the first time, verifies that the acacetin can obviously inhibit the replication of the white spot syndrome virus in the procambarus clarkia body, and simultaneously improves the survival rate of the procambarus clarkia infected by the white spot syndrome virus. The acacetin has a good antiviral effect on the white spot syndrome virus in crustacean aquaculture, and can be applied to prevention and treatment of aquatic animal diseases caused by the white spot syndrome virus.

The invention provides an artificial breeding method of wild sal-family large yellow croaker, and belongs to the technical field of aquatic animal breeding. The invention provides an artificial breeding method of wild sal-family pseudosciaena crocea, which comprises the following steps: domesticating the wild sal-family pseudosciaena crocea, then carrying out intensive cultivation, and after the wild sal-family pseudosciaena crocea is cultivated until gonad is mature, carrying out artificial spawning induction and natural fertilization on the obtained parent fish of the sal-family pseudosciaena crocea to obtain fertilized eggs; the fertilized eggs are subjected to incubation and fry cultivation, and sal-family large yellow croaker fries are obtained; the artificial spawning method comprises the step of injecting hormone into parent fish in two steps. According to the method, favorable conditions are created for large-scale breeding of the sal-family pseudosciaena crocea, and a large number of artificially bred sal-family pseudosciaena crocea fry groups can be obtained by adopting the method.

The present invention provides a Chinese medicine composition for preventing and treating "milk disease" of Chinese mitten crab, and a preparation method and application thereof, and the present invention belongs to the technical field of Chinese medicine for aquatic animals. The Chinese medicine composition provided by the present invention comprises the following raw materials in parts by weight: 1 to 10 parts of coptis root, 1 to 10 parts of sappan wood, 1 to 10 parts of pomegranate peel, 1 to 10 parts of cloves, 1 to 10 parts of scutellaria and 1 to 10 parts of schisandra chinensis. The Chinese medicine composition provided by the present invention is for the prevention and treatment of "milk disease" of Chinese mitten crab caused by Metschnikowia bicuspidatum, and provides an efficient, safe and practical method, with an inhibition rate of more than 99.67%, low cost, simple preparation process, high raw material utilization rate, easy industrialization, and good development value and market prospects.

The invention discloses a recombinant bacterium for high-yield production of selenomethionine and a preparation method of the recombinant bacterium, and belongs to the technical field of genetic engineering. According to the invention, selenium-rich region (Enshi) soil and withered grass are taken as materials, a bacillus subtilis strain with high selenomethionine synthesis capability is separated and screened, the bacillus subtilis strain is taken as an original strain, and a recombinant strain of high-expression SeMet synthesis key gene thioredoxin reductase (TrxRB) is constructed by utilizing an expression vector pHT43, so that high-yield SeMet engineering bacteria are obtained. The bacillus subtilis rich in selenomethionine and the recombinant bacteria thereof can be used as feed additives, and can be used for supplementing SeMet and improving the feed conversion rate in the production of livestock and poultry and aquatic animals.

The invention relates to the field of aquatic feed, and provides a low-temperature adhesive type aquatic feed particle composition and a preparation method thereof. The composition is composed of puffed soybean meal, flour, fish meal, low-temperature directed enzyme, microcrystalline cellulose, modified zein nanoparticles, a protein adhesive and a modified zein ethanol water solution. The preparation method comprises the following specific steps: reacting soybean protein isolate with cross-linking enzyme to generate a protein adhesive, reacting proanthocyanidins with gallic acid to generate gallic acylated proanthocyanidins, and carrying out multi-step treatment on zein. Experimental results show that the composition has excellent water resistance and oxidation resistance, and the growth efficiency and the survival rate of aquatic animals are remarkably improved. The preparation method is simple, the cost is low, heat-sensitive substances in the feed are not damaged, and an economical, efficient and nutrient-rich feed solution is provided for aquaculture.

The invention provides a method and a device for efficiently proliferating sepiella maindroni by combining grazing and raising, and belongs to the field of aquatic animal proliferation. An efficient breeding method for sepiella maindroni integrating grazing and breeding comprises the following steps that an appropriate sea area is selected, fertilized eggs are bred in a breeding facility laid in the downstream direction of a normal fish breeding net cage, namely an empty net cage containing an artificial floating reef, the periphery of the empty net cage is provided with an algae-mussel flow blocking ecological rope, and a cover net is arranged above the empty net cage; the newly hatched cuttlefish can stay in the proliferation facility empty net cage for a long time due to the characteristics of loving reefs and drilling the net and strong space awareness, and slowly escape into natural water after gradually adapting to the proliferation environment. According to the proliferation method, cuttlefishes gradually adapt to the natural environment of the sea area, meanwhile, cuttlefish larvae are well protected enough in the fragile incubation initial stage through protection measures such as net cages, and therefore the proliferation survival rate and the maintenance efficiency are improved, and efficient proliferation is achieved.

The invention relates to the technical field of aquatic animal living body transportation, and discloses a sea urchin long-distance transportation synergistic method based on dry transportation and anhydrous volatile anesthesia, which comprises the following steps: placing sea urchin in a closed container, using a natural volatile substance as an anesthetic, loading and volatilizing through an adsorption material, and performing anhydrous anesthesia on the sea urchin; the anesthetized sea urchin is inversely placed on a fixing support with a mesh structure in a transportation container in the posture that the mouth face faces upwards and the thorns face downwards, and the sea urchin is prevented from rolling over in the transportation process through the self-locking effect of the thorns and meshes; a functional interlayer is arranged in the fixing support, and a temperature control material and a moisturizing material are placed in the functional interlayer at the same time to maintain the temperature and humidity of the transportation environment; after transportation, the sea urchins are placed in seawater containing the cactus extract for initial recovery and then transferred to flowing natural seawater to complete unified recovery, a whole-process collaborative protection chain of anhydrous anesthesia loss prevention, dry transportation steady state maintenance and rapid water phase restoration can be constructed, and the dry transportation survival rate and quality are remarkably improved.

The invention discloses marinobacter hydrocarbonoclastici SN-1 and a culture method and application thereof, the marinobacter hydrocarbonoclastici SN-1 is marinobacter hydrocarbonoclastici SN-1, is preserved in China Center for Type Culture Collection on August 7, 2024, is located in Wuhan University, Wuhan, China, and has a preservation number of CCTCC NO: M 20241755, and the preservation number of the marinobacter hydrocarbonoclastici SN-1 is CCTCC NO: M 20241755. The invention also discloses application of the marinobacter hydrocarbonoides SN-1 or the microbial inoculum in degradation of ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and / or active phosphate concentration in seawater. The seawater body is a mariculture water body, mariculture tail water or an offshore water body, the salinity of the seawater body is 10-40, and the inoculum size of the mariculture SN-1 is 2%. The marinobacter hydrocarbonoides SN-1 disclosed by the invention can remove ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, total nitrogen, active phosphate and total phosphorus in a seawater water body in an aerobic environment with relatively high salinity, has no toxic effect on aquatic animals, and can be applied to improvement and ecological restoration of a seawater water environment.

The invention discloses an application of marine streptomyces SH110 and a source compound thereof, the marine streptomyces SH110 has a preservation number of GDMCC (China General Microbiological Culture Collection Center) NO.66668, and the preservation date is July 14, 2025; the invention also discloses application of the marine streptomyces SH110-derived compound in preparation of a medicine for preventing and treating streptococcus and vibrio diseases. The marine streptomyces source compound has efficient vibrio and streptococcus resisting activity, the vibrio and streptococcus resisting activity is not reported, and the marine streptomyces source compound has new application. The marine streptomyces source compound is used for preparing the medicine for preventing and treating the vibrio and streptococcus diseases, especially for preparing the medicine for preventing and treating the vibrio and streptococcus diseases of aquaculture animals, the safety is high, and the treatment effect is good.

The invention relates to a super-multiplex digital PCR (polymerase chain reaction) detection method for simultaneously detecting multiple pathogens of shrimps and application of the super-multiplex digital PCR detection method. According to the digital PCR primer, the probe, the kit and the detection method for shrimp pathogen detection, pathogens aiming at 18 shrimp pathogens can be rapidly, accurately and ultrasensitively detected. By detecting pathogens in the prawns, the health condition of the prawns in intensive prawn culture can be evaluated in time, disease transmission can be prevented, intervention measures can be taken in time, the risk of disease transmission to prawn groups in intensive culture can be reduced, serious economic loss can be avoided, and the application value is extremely high. Accurate identification of specific pathogens in a shrimp sample is very important for designing a proper control strategy, because each pathogen may require different prevention and management methods, drug use conditions can be effectively reduced. The shrimp pathogen detection can improve the culture benefit, and is also beneficial for developing the epidemic disease prevention and control work of aquatic animals and the inspection and quarantine monitoring plan of main aquaculture variety offspring seeds.

The invention provides an artificial propagation method of Sinilabeo wuyi, and belongs to the technical field of aquatic animal breeding. The artificial propagation method of the Sinilabeo wuyi comprises the steps of parent fish classification, parent fish rearing, artificial spawning induction, natural insemination, artificial insemination, fertilized egg incubation, fry rearing, juvenile fish rearing and the like. The oxytocic is reasonable in compatibility, the injection time of the oxytocic is accurate, the oxytocic rate, the fertilization rate and the hatching rate are increased, and damage to parent fishes is small. According to the method, feeding methods in different stages of fry breeding are also defined, the survival rate of the sinilabeo wuyi fry breeding stage is increased, and a technical support is laid for artificial breeding of the endangered species sinilabeo wuyi.