68 results about "Aquatic animal" patented technology

The application provides a new bacteriophage for preventing and treating vibrio diseases of aquatic animals and application thereof, the bacteriophage is preserved in China Center for Type Culture Collection on September 4, 2020, and the preservation number is CCTCC M 2020465. The bacteriophage is a new species of bacteriophage of the Caudovirales class and Queuovirinae subfamily, the whole genome of the bacteriophage is OP331229.1 in the NCBI official website GeneBank. The bacteriophage has a wide phage spectrum, can crack 6 different species and genera of vibrio at the same time, can crack 45 strains of Vibrio parahaemolyticus, 22 strains of Vibrio harveyi, 17 strains of Vibrio alginolyticus, 13 strains of Vibrio vulnificus, 11 strains of Vibrio campbellii and 9 strains of Vibrio cholerae at the same time, can more effectively solve the problem of multiple bacterial disease infections in aquaculture, and can be used as a microecological preparation for preventing and treating vibrio diseases such as Vibrio parahaemolyticus, Vibrio harveyi and Vibrio alginolyticus of aquatic animals.

The application discloses application of pyrroloquinoline quinone (PQQ) or a derivative thereof in preparation of a product for relieving or preventing blue-green algae toxin poisoning of yellow catfish, and belongs to the technical field of aquatic animal feed. The PQQ or the derivative thereof can be used as an aquatic feed additive or a medicine, and is added in an amount of 3-6 mg / kg of feed to feed yellow catfish, so as to effectively relieve pathological damage of the liver of the yellow catfish caused by blue-green algae toxin through various ways such as improving key enzyme activity of liver function, inhibiting liver oxidative stress, relieving inflammatory reaction and reducing structural damage of tissues. The PQQ is a new food raw material approved in China in 2022, has advantages of high safety, good water solubility, moderate cost, similar dosage to that of feed vitamins and the like, and provides a safe, economical and convenient new nutritional intervention strategy for blue-green algae toxin poisoning of the yellow catfish, and has a wide industrial application prospect.

The present invention provides a high-protein bio-fermented aquatic feed and a preparation method thereof. The aquatic feed comprises the following raw materials in parts by weight: 20-30 parts of imported fish meal, 10-15 parts of compound minerals, 5-10 parts of bioactive peptides, 5-10 parts of lysine, 5-10 parts of compound trace elements, 3-8 parts of compound vitamins, 3-6 parts of herbal extracts, 2-5 parts of compound probiotics, 2-4 parts of immune polysaccharides, and 1-3 parts of compound enzymes; the herbal extracts are prepared by fermenting astragalus membranaceus, isatis root, liquorice root, calotropis gigantea, ventilago leiocarpa benth, clove, rhizoma chuanxiong, and galangal officinale. By organically integrating imported fish meal, astaxanthin-peptide chelate, herbal extracts, compound immune polysaccharides, compound probiotics and various nutrients, the present invention realizes the synergistic effect of "promoting feeding - enhancing digestion - strengthening liver and gall - enhancing immunity - resisting stress - inhibiting bacteria - stabilizing water quality", significantly improves the growth rate, survival rate and comprehensive breeding benefit of crustacean aquatic animals, and has good economic value and application prospect.

This invention belongs to the field of microbial technology and discloses a strain of *Citrobacter freundii* bacteriophage PRF337, its bacteriophage composition, and its applications. The *Citrobacter freundii* bacteriophage PRF337 was deposited on May 26, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46489. The bacteriophage PRF337 exhibits broad-spectrum bactericidal activity against *Citrobacter freundii*, capable of lysing *Citrobacter freundii* strains containing different virulence genes and from different sources. It also possesses certain temperature and pH stability and can be used as an active ingredient in pharmaceutical preparations, environmental disinfectants, and water purifiers. It can effectively control *Citrobacter freundii* infection in aquatic animals such as fish and shrimp in aquaculture environments and can also be used for the purification of pathogens in aquatic environments. It is safe to use and effectively avoids the problems of antibiotic residues and pathogen resistance caused by antibiotic use.

This invention discloses a powder containing doxycycline hydrochloride and potassium clavulanate for aquaculture, its preparation method, and its application, belonging to the field of biotechnology. This invention combines doxycycline hydrochloride and potassium clavulanate to enhance the antibacterial ability of aquatic animals against drug-resistant bacteria, thereby better treating various diseases caused by drug-resistant bacteria in aquatic animals. Further experiments demonstrate that potassium clavulanate can significantly reduce bacterial resistance and enhance the antibacterial activity of doxycycline hydrochloride. The preparation of a composite powder using the combination of doxycycline hydrochloride and potassium clavulanate is simple, uses readily available raw materials, and is suitable for large-scale industrial production. Its application in the treatment of aquatic animal diseases, verified through challenge experiments, demonstrates the effectiveness of this composite powder in treating bacterial infections in aquatic animals. It has broad application prospects.

The present application relates to the field of feed additive, and discloses a feed additive, a preparation method and application thereof.The feed additive contains astaxanthin, curcumin, vitamin C palmitate, vitamin E, vitamin D3, piperine and a carrier.The preparation method comprises the following steps: mixing astaxanthin, curcumin, vitamin C palmitate, vitamin E, vitamin D3, piperine and the carrier.The feed additive can significantly improve the stress resistance, immunity, antioxidant capacity and disease resistance of aquatic animals, and has significant economic benefits.

The utility model provides a kind of farmland type ecological buffer zone structure, it is related to agricultural ecological environment management technical field, including land buffer area one, ecological regulation pond, land buffer area two and concave green land, land buffer area one, ecological regulation pond, land buffer area two and concave green land are sequentially distributed from farmland side to river embankment side, land buffer area one and land buffer area two are planted with arbor shrub and grass flower mat, the ecological regulation pond is sequentially provided with impermeable layer, adsorption layer and plant layer from bottom to top, the concave green land is laid with buffer base, the concave green land is planted with herbaceous plant and shrub. This structure effectively intercepts farmland non-point source pollution, reduces river pollutant, helps to strengthen river self-purification capacity, promotes aquatic animal and plant diversity development, and guarantees ecological benefit to play well.

This application discloses a method for periodically feeding vine tea ultrafine powder in aquaculture and its application. The method includes the following steps: mixing vine tea ultrafine powder into aquatic animal feed at an addition rate of 1% to 8% of the total feed weight, and feeding it using a periodic feeding pattern; the periodic feeding pattern consists of continuous feeding for 7 days, followed by a 7-21 day break, which constitutes one cycle. This application, by pulverizing vine tea to an optimal particle size of 300 mesh, and by optimizing the optimal addition rate of 4% and the periodic feeding strategy of "7 days of continuous use, 14 days of break," fully utilizes the antioxidant and immune-enhancing effects of vine tea while effectively avoiding the potential liver and kidney burden and immunosuppression risks associated with long-term continuous use. Large-scale application trials show that this method achieves a protection rate of 52.91% to 65.14% in various aquatic animals, including crucian carp, largemouth bass, whiteleg shrimp, carp, and tilapia, demonstrating significant economic benefits and promising prospects for industrial application.

A new antibacterial peptide of Japanese eel A1 Cath2b 143‑174 and its application, belong to the field of biotechnology. The antibacterial peptide is a Cathelicidin family antibacterial peptide of Japanese eel A1 The 143-174 amino acid fragment of Cathelicidin 2b is named as Cath2b A1 Cath2b 143‑174 , and the amino acid sequence is shown as SEQ ID NO. 3. The antibacterial peptide has high inhibition / killing activity on various aquatic pathogenic bacteria, has excellent thermal stability and salt ion tolerance, and has no obvious toxicity to host cells; it plays a bactericidal role by destroying the bacterial cell membrane, and can also attract host immune cells to enhance immunity. The antibacterial peptide can be applied in the preparation of aquatic animal bacterial disease prevention and control drugs. It can solve the problems of drug resistance and drug residue caused by the abuse of traditional antibiotics, and is convenient to prepare, has strong adaptability, and is suitable for the prevention and control of bacterial diseases of aquatic animals.

The application discloses a fishing device and a method thereof. The fishing device comprises a box body, a cover body and a plurality of fishing baskets. The box body has an opening for aquatic animals to enter the box body. The cover body is detachably connected above the box body. The box body has a plurality of trapping chambers arranged in sequence in a direction away from the opening. Each trapping chamber is provided with a plurality of trapping holes on a side facing the opening. In the direction away from the opening, the hole diameters of the trapping holes corresponding to the trapping chambers decrease in sequence. The fishing baskets correspond to the trapping chambers one by one. The fishing baskets are placed in the corresponding trapping chambers and are used for collecting aquatic animals entering the trapping chambers. Aquatic animals of different sizes enter the trapping chambers with corresponding hole diameters and stay in the respective fishing baskets. The fishing baskets can be directly obtained by opening the cover body, and manual sorting is not needed after being taken out of water, which is suitable for large-scale breeding operations.

The application discloses a breeding water pathogenic bacteria detection chip, device, primer probe group, kit and method, relates to the technical field of aquatic animal pathogenic bacteria detection, and the detection chip comprises a nucleic acid extraction chamber, which can extract pathogenic bacteria nucleic acid solution in breeding water; a reagent storage chamber is communicated with a detection chamber at one end, can store a reaction solution, and can deliver the reaction solution into the detection chamber; a pipetting module is arranged between the detection chamber and the nucleic acid extraction chamber, can deliver a set amount of pathogenic bacteria nucleic acid solution into the detection chamber; and a fluorescence detection module of the detection device can detect fluorescence signals of mixed pathogenic bacteria nucleic acid solution and reaction solution in the detection chamber. The breeding water pathogenic bacteria detection chip, device, primer probe group, kit and method provided by the application can realize on-site rapid detection of breeding water pathogenic bacteria.

The present application relates to the technical field of aquatic animal cell culture, and particularly relates to a rapid enrichment and separation method of Plectrypops leopardus gonadal somatic cells. The method comprises the following steps: selecting healthy Plectrypops leopardus for anesthesia; taking gonadal tissue, and after aseptic treatment, cutting, centrifugal washing and filtration, inoculating in a culture medium for primary culture to obtain gonadal cells migrated from the tissue block, and after trypsin-EDTA digestion and centrifugal washing, using 1xPBS containing polysorbate for treatment; after blocking, antibody incubation and washing, verifying the labeling effect by a fluorescence microscope to realize specific enrichment of gonadal somatic cells; after filtration and purification of the enriched fluorescent labeled cells, placing the cells under a fluorescence microscope, accurately picking fluorescent positive cells under a fluorescence field, and transferring the cells to a culture dish containing complete culture medium, inoculating the separated fluorescent positive cells in optimized complete culture medium for purification culture, so that the cells adhere to the wall and realize stable subculture.

The application belongs to the technical field of aquatic animal molecular marker assisted breeding, and particularly relates to a KASP molecular marker combination for detecting the ammonia nitrogen tolerance performance of Takifugu rubripes and application thereof. The KASP molecular marker combination comprises five SNP sites significantly related to the ammonia nitrogen tolerance performance of Takifugu rubripes, which are located at 9542716, 9544701, 9546090, 9547752 and 9551066 sites of chromosome 11 of Takifugu rubripes reference genome, and the base polymorphism types thereof are C / A, T / C, T / C, G / A and G / C respectively, and the corresponding ammonia nitrogen tolerance advantage genotypes are AA, CC, CC, AA and CC in turn. When the to-be-detected individual simultaneously has the ammonia nitrogen tolerance advantage genotypes of the five SNP sites, it is determined as an ammonia nitrogen tolerance individual. The application can be used for detecting the ammonia nitrogen tolerance performance of Takifugu rubripes and molecular marker assisted breeding, and is helpful for early and efficient screening of ammonia nitrogen tolerance germplasm.

This invention provides the application of Usnea powder, Usnea acid, or sodium usneate (or Usnea powder, Usnea acid, or sodium usneate as the sole active ingredient) in the preparation of broad-spectrum antiparasitic drugs for aquatic animals, drugs for treating complications caused by parasitic infections, feed additives, or functional feeds. By utilizing Usnea, Usnea extract, Usnea acid, and Usnea salts, various parasites, including ciliates, worms, and protozoa, that infest the surface and interior of aquatic animals during aquaculture can be eliminated or killed, thus maintaining the health of farmed animals, improving the success rate of aquaculture, achieving broad-spectrum and highly effective prevention and control of aquatic animal parasitic diseases, reducing the use of chemical drugs, and providing good aquaculture, ecological, and social benefits. It is safe, broad-spectrum, and highly effective, facilitating large-scale promotion.

This invention discloses a molecular marker for identifying the body size of bighead carp and its application, belonging to the field of molecular breeding technology for aquatic animals. The molecular marker is a SNP site on the cathepsin Bb gene, whose nucleotide sequence exhibits a G / C polymorphism at position 1907, named g.1907.G>C. This SNP site is located on intron 4 of the cathepsin Bb gene. The genotype of this SNP site is significantly correlated with the body weight of bighead carp, and individuals with the CC genotype have significantly higher body weights than those with the GC genotype. This invention can rapidly and accurately screen for bighead carp individuals with superior body size, providing technical support for marker-assisted breeding of bighead carp and improving breeding efficiency.

The present application relates to the field of agricultural ecological breeding technology, and specifically discloses a kind of rice field co-production ecological comprehensive breeding method, comprising: rice field reconstruction, excavate field-shaped ecological ditch to form middle rice area and ditch breeding area;Equipment installation, erect the three-dimensional ecological habitat platform of lifting in the middle rice area, platform is connected to column by lifting drive mechanism;Species release, release aquatic animal seedling after rice and grass return green tillering period;Intelligent scheduling, platform height is controlled based on environmental data collected by Internet of Things sensor;Circulation co-production, utilize aquatic animal to move soil, remove insects, increase oxygen, excrement fertilize field, and aquatic animal independently enters and exits platform until harvest.The present application realizes the dynamic space cooperation of rice and animal activity through the lifting platform, constructs three-dimensional ecological channel, automatically responds to high temperature, night, oxygen deficiency and other scenes in combination with intelligent scheduling, solves the space conflict problem of animal and rice, and realizes energy self-sufficiency and resource recycling.

This utility model discloses a suspended feeding aquaculture device, belonging to the field of aquaculture equipment technology. It includes a floating plate, a support frame, a roller, and a first guide rail. The upper left end of the floating plate is fixedly connected to the first guide rail, and a servo motor is fixedly installed at the rear end of the first guide rail. A threaded rod is fixedly connected to the power output end of the servo motor, and a first slider is threaded onto the threaded rod. A first rotating shaft is rotatably connected to the right side of the first slider, and a gear is fixedly connected to the first rotating shaft. A rack is meshed below the gear. A roller is fixedly connected to the right end of the first rotating shaft, and a second rotating shaft is fixedly connected to the right side of the roller. A feed pipe is rotatably connected to the right end of the second rotating shaft. The device uses a servo motor to drive the roller to move back and forth, and the gear and rack work together to achieve roller rotation. This allows the feed outlet to open and close periodically, and the feed is evenly sprinkled into the receiving bag as the roller rotates, and then naturally diffused into the aquaculture area, improving the uniformity of feeding.

This invention discloses an AI-2 quenching enzyme and its application. The AI-2 quenching enzyme is protein QQ-5, with the amino acid sequence shown in SEQ ID NO:1. Experiments have demonstrated that protein QQ-5 possesses AI-2 quenching enzyme activity, which can significantly improve the disease resistance of aquatic animals to Vibrio, manifested in effectively increasing the survival rate of aquatic animals infected with Vibrio and delaying the time to death. Therefore, protein QQ-5 plays an important role in the control of AI-2 family signaling-mediated pathogenic bacteria in aquatic animals. This invention has significant application value.

This invention discloses a device and computer program product for automatically detecting the health status of aquatic animals based on spatiotemporal information. The invention constructs the STAFDD spatiotemporal aquatic animal health status detection framework, specifically based on an improved YOLOv8 detector integrating an SPD-CBS module, a coordinate attention mechanism, and a small target detection head to detect images or videos. It employs the ByteSort algorithm with integrated ReID features to achieve stable multi-target tracking, ensuring coherent trajectory extraction. Simultaneously, it introduces an LSTM-based behavior analysis module to effectively identify abnormal movement patterns in diseased fish, and performs joint diagnosis by combining appearance features and behavioral cues. This invention considers both spatial features and temporal information, contributing to more accurate and robust identification of aquatic animal health status.

This invention discloses the application of Terminalia chebula in the prevention and treatment of Ichthyophthirius multifiliis (white spot disease) in aquatic animals. Toxicity experiments on grass carp kidney cells (CIK) showed that the maximum non-toxic concentration (NDC) of the aqueous extract of Terminalia chebula against CIK cells was 500 μg / mL. The inhibition of Ichthyophthirius multifiliis predator survival by the aqueous extract was dose-dependent, and the predator mortality rate reached 100% at a concentration of 100 mg / L (μg / mL). Furthermore, the median lethal concentration (LD50) of the aqueous extract against Ichthyophthirius multifiliis predators was calculated to be 14.577 mg / L. This invention is the first to propose the application of Terminalia chebula in inhibiting the survival rate of Ichthyophthirius multifiliis predators in fish at a non-toxic concentration to cells, providing a novel drug for the prevention and treatment of Ichthyophthirius multifiliis infection in aquatic animals. Terminalia chebula is a natural medicinal plant, environmentally friendly, and has the advantages of reducing the risk of drug residues.

This invention relates to the field of aquaculture technology, and in particular to a biological attractant based on Schizochytrium fermentation broth and its preparation method; the biological attractant based on Schizochytrium fermentation broth is obtained by processing the fermentation broth after oil extraction from Schizochytrium; the fermentation broth after oil extraction from Schizochytrium is the residual fermentation broth after supercritical CO2 oil extraction or enzymatic hydrolysis oil extraction from Schizochytrium; the preparation method includes: pretreatment of fermentation broth, sterilization and stabilization treatment, concentration and regulation, and filling and storage; the three core components in the fermentation broth work synergistically, umami amino acids quickly trigger the taste perception of aquatic animals, algal polysaccharides slowly release odor molecules to prolong the feeding attraction time, and DHA enhances natural tropism, significantly improving the feeding induction rate of Litopenaeus vannamei, and maintaining a stable feeding attraction effect even in rainy and low-temperature environments; relying on the natural viscosity and structural characteristics of algal polysaccharides, there is no need to add stabilizers such as xanthan gum.

This invention discloses an antimicrobial peptide, Cih1, and its applications. The amino acid sequence of antimicrobial peptide Cih1 is shown in SEQ ID NO.1. The antimicrobial peptide Cih1 provided by this invention is a cationic peptide, rich in cysteine ​​(-cys) residues, which can form an amphiphilic β-sheet secondary structure. This structure endows it with excellent antibacterial and antiviral activity, and can effectively inhibit the proliferation and invasion of pathogens in aquaculture water. Based on the broad-spectrum resistance of antimicrobial peptide Cih1 to pathogens, it can help aquatic animal hosts resist infection by various pathogens, and has broad application prospects in the field of anti-infective drug development for aquaculture.

The application discloses an aquatic pet cloud primary screening and on-site treatment whole-cycle management system and method based on artificial intelligence, which comprises an offline service terminal used for collecting multi-modal data of aquatic pets and performing on-site service operations; a cloud hub system is in communication connection with the offline service terminal through a mobile communication network, is used for receiving offline service terminal data and performing data processing, decision-making and resource scheduling processing, and comprises an AI computing cluster, which is used for analyzing the multi-modal data to generate diagnosis suggestion information; an intelligent service scheduling module is used for acquiring the diagnosis suggestion information and on-site resource states fed back by the offline service terminal, generating and issuing a service execution scheme to the offline service terminal; and the offline service terminal receives the service execution scheme and performs corresponding on-site service operations. The application constructs an aquatic pet multi-modal AI primary screening diagnosis module based on data resources accumulated by a national aquatic animal pathogen library, and significantly improves disease identification accuracy and diagnosis efficiency.

This invention relates to the field of microbial technology, specifically to a highly efficient lysing Vibrio alginolyticus phage derived from abalone, its composition, and its applications. The phage, named RKP-VA24471, has been deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 46426. The phage has a titer of not less than 1 × 10⁻⁶. ^10 With a PFU / mL concentration and an optimal MOI of 0.01, this phage exhibits advantages such as a short latency period, high outbreak rate, good genetic stability, and a wide adaptability to temperature and pH. Lysis spectrum experiments show that this phage has broad-spectrum lytic activity against various Vibrio species, including *Vibrio alginolyticus* and *Vibrio parahaemolyticus*, with an overall lysis rate of 80.6%. Animal experiments have demonstrated that this phage significantly improves the survival rate of abalone infected with *Vibrio alginolyticus*, outperforming antibiotics and fish-derived phages. The phage and its composition provided by this invention can be used for the prevention and treatment of vibriosis in aquatic animals such as abalone, offering a highly efficient and environmentally friendly biological control solution as an alternative to antibiotics.

This invention relates to an enzymatically hydrolyzed protein composition as a substitute for fishmeal and its preparation method, belonging to the field of feed technology. The enzymatically hydrolyzed protein composition comprises the following raw materials: animal protease hydrolysate, brewer's yeast extract, bile acids, sodium butyrate, sodium selenite, and fructooligosaccharides. This application selects cricket powder and defatted mealworm powder as animal protein sources. Simultaneously, chitinase, alkaline protease, and neutral protease are used sequentially to enzymatically hydrolyze the cricket and mealworm complex. The cricket powder and defatted mealworm powder after enzymatic hydrolysis effectively eliminate anti-nutritional factors in the original insect feed, and have high protein utilization, effectively releasing nutrients and improving nutritional value. Based on this, brewer's yeast extract and bile acids are added for compounding, which synergistically promotes weight gain and growth in aquatic animals. Furthermore, this application uses sodium butyrate, sodium selenite, and fructooligosaccharides in combination, which effectively gives the prepared composition significant antioxidant effects from different dimensions.

This invention proposes a feed additive, feed, and preparation method for repairing intestinal damage in aquatic animals, relating to the field of aquatic feed technology. This invention aims to address the problem of intestinal damage in aquatic animals caused by various factors in aquaculture. The feed additive of this invention comprises, by weight, 1-3 parts selenium nanoparticles, 5-10 parts astragalus polysaccharide, and 3-5 parts γ-aminobutyric acid (GABA). The compound aquatic feed additive and its formulated feed provided by this invention, through the optimized combination and synergistic effect of selenium nanoparticles with astragalus polysaccharide and GABA, improve the repair capacity of intestinal damage in aquatic animals through multiple pathways and levels, effectively alleviating the adverse effects of decreased survival rate caused by environmental stress and significantly improving the production performance of aquatic animals. This invention solves the problems of low bioavailability and narrow toxicity threshold of traditional selenium preparations, achieving safe and efficient intestinal protection.

The application belongs to the technical field of aquaculture, and particularly relates to a pond zero-waste discharge circulating water breeding method. The pond zero-waste discharge circulating water breeding method comprises the following steps: step S10, dividing a pond water surface into a high-oxygen area, a low-oxygen area and a corridor, arranging an oxygenator in the high-oxygen area, and connecting the high-oxygen area and the low-oxygen area through the corridor so that aquatic animals can freely select habitat space among the high-oxygen area, the low-oxygen area and the corridor; step S20, putting aquatic animals in the pond water surface; step S30, starting the oxygenator as needed to ensure the dissolved oxygen content of the high-oxygen area; and step S40, feeding as needed, and regularly pumping high-oxygen area bottom sewage into the low-oxygen area. The breeding method has high feed conversion rate, simple management, stable water quality all year round, realizes zero discharge of breeding wastewater and also realizes zero discharge of solid pollutants, and does not need to clean pollutants.

The application discloses a production technical process of gonad delayed development of large-mouth bass, and belongs to the technical field of aquatic animal biological breeding. The application creates new germplasm of large-mouth bass with significantly improved growth speed, significantly decreased gonad index and better muscle quality and flavor by parent cultivation, design of antisense RNA sequence and amplification, artificial insemination of the large-mouth bass after preparation of transfection reagent and introduction of the antisense RNA sequence of a key gender development gene, thereby providing technical support for promoting high-quality and sustainable development of the large-mouth bass industry.