556 results about "Aquatic animal" patented technology

The invention discloses a photodynamic inactivation parameter adjusting method based on hybrid fuzzy-PID control, relates to the technical field of parameter fuzzy control, and aims at the problem that photodynamic inactivation efficiency and aquatic organism safety in aquaculture are difficult to consider at the same time through multi-sensor fusion and environment prediction. Then, a fuzzy-PID self-adaptive putting technology is adopted to be coupled with an illumination strategy, and accurate matching of the photosensitizer dosage and the light intensity is achieved; water body fluctuation and pathogen diffusion are dealt with through real-time closed-loop and multi-scene collaborative scheduling; multi-dimensional index evaluation is utilized, reinforcement learning is introduced to carry out self-learning optimization, and putting and illumination parameters are continuously improved; and finally, the method is expanded to large-scale distributed control and cloud big data training, and efficient inactivation and flexible deployment can be kept in different breeding scenes. Pathogens can be effectively prevented from being rapidly diffused in an adverse environment, damage to aquatic animals caused by excessive accumulation of reactive oxygen species (ROS) is reduced, and meanwhile energy consumption and resource waste are reduced.

The invention belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding, and particularly discloses an SNP (Single Nucleotide Polymorphism) molecular marker remarkably related to grass carp body weight traits and application thereof. The SNP molecular marker is shown as follows: (1) SLG1123629389 with the polymorphic base being T / C, (2) SLG1127776299 with the polymorphic base being A / G, (3) SLG1127776356 with the polymorphic base being G / A, and (4) SLG1235362696 with the polymorphic base being T / C. The invention also discloses application of the SNP molecular marker, the amplification primer or the kit in breeding grass carp with excellent growth traits. The SNP molecular marker disclosed by the invention can be used for early breeding of grass carp with excellent growth traits, so that the breeding workload is greatly reduced, the breeding time can be remarkably shortened, the breeding process is accelerated, the breeding efficiency is improved, and the breeding cost is reduced; the method has important guiding significance for improving the production performance of the grass carp, reducing the culture cost of the grass carp and increasing the culture income, and is suitable for popularization and application.

The invention belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker remarkably related to growth traits of grass carp and application of the SNP molecular marker. The SNP molecular marker is located at the position of 23608308bp of the eleventh chromosome (SLG1123608308) of the grass carp, and is Tgt; and A basic group mutation. Experiments show that the SNP molecular marker developed by the invention has significant influence on four growth traits (Plt; 0.05), and the allele A has more excellent growth traits. The invention discloses application of an SNP (Single Nucleotide Polymorphism) molecular marker, an amplification primer or a kit in breeding grass carp with excellent growth traits. The SNP molecular marker provided by the invention is not limited by the age, sex and the like of the grass carp, can be used for breeding grass carp varieties with excellent growth traits, and even can be accurately screened after being hatched, so that the breeding process of the grass carp varieties with excellent growth traits is greatly accelerated.

The invention relates to the technical field of data processing, and discloses an aquaculture intelligent monitoring method and system based on the Internet of Things. The method comprises the following steps: collecting pond environment parameters and acoustic signals through layered arrangement of sensors, and obtaining multi-pond linkage data through wireless transmission; performing Fourier transform processing on the data to generate biology-environment coupling data; the water quality correlation degree between the ponds is calculated, and regional balance control data are formed; biological activity rules are analyzed, and biological rhythm feeding data are obtained; checking the carbon footprint to generate low-carbon operation data; and performing multi-dimensional correlation analysis to form ecological breeding optimization data. According to the method, the layered and partitioned ecological sensing network is constructed, collaborative analysis is performed on multi-pond environment data, and intelligent management of the breeding process is realized in combination with biological rhythm characteristics and carbon emission control requirements of aquatic animals, so that the breeding efficiency is improved, and the carbon emission intensity is reduced.

The invention relates to the technical field of aquaculture, and discloses an ecological aquaculture system, which comprises a culture pond as a basic component of the whole device and used for bearing and assembling a central data processing system and a subordinate lower structural component thereof; the central data processing system is used for receiving, processing and storing data from each monitoring module; the multi-parameter water quality monitoring module monitors key water quality indexes including dissolved oxygen, temperature, pH value, ammonia nitrogen content and nitrite content in real time; the aquatic animal growth monitoring module is used for measuring growth parameters including body length and body weight of aquatic animals through an image recognition technology; through the multi-parameter water quality monitoring device, the culture environment and the state of the aquatic animals can be mastered in real time, so that accurate management is performed, and the disease early warning device can find disease risks in an early stage, take prevention and treatment measures in time and reduce the influence of diseases on the growth of the aquatic animals.

The invention discloses a low-carbon freshwater fish feed added with fermented bamboo powder and application of the low-carbon freshwater fish feed, and belongs to the technical field of aquatic animal feeds. The low-carbon freshwater fish feed capable of reducing the fat rate of fish bodies and / or reducing nitrogen and phosphorus emission is prepared from the fermented bamboo powder, and the content of vitamins, enzymes and growth factors in the feed can be increased by adding the fermented bamboo powder, so that the freshwater fish bodies have good anti-pathogenic activity; the absorption and utilization of the freshwater fish on nutritional ingredients and fiber-rich ingredients in the feed are improved, so that the aim of reducing the emission of nitrogen and phosphorus is fulfilled; meanwhile, the fermented bamboo powder is used for partially replacing soybean meal in the freshwater fish feed, so that the dosage of feed raw materials such as the soybean meal is reduced, the intestinal health of the freshwater fish is improved, the muscle quality of the freshwater fish is improved, the earthy smell in muscles is reduced, the body type of the freshwater fish is also improved, and the fat content in abdominal muscles is reduced.

The invention relates to systems, apparatus, and methods of objects such as aquatic animals (e.g. fish) in a harvesting environment. There may be provided A system for monitoring aquatic animals (e.g. fish) at harvest (e.g. in real time) comprising: a (e.g. electric) stun station; a dewatering station; optionally, a processing station (e.g. a post-stun processing station to elicit death, such as percussive stun and / or bleed, and / or ice treatment, and so on); at least one imaging station; and, at the at least one imaging station, an image acquisition unit comprising: at least one camera module configured for capturing images of aquatic animals post (e.g. electric) stun as these pass through the imaging station; an image processing unit of, or operably coupled to, the image acquisition unit comprising: at least one microprocessor; at least one storage medium comprising one or more programs, the one or more programs configured for execution on the at least one microprocessor, the one or more programs comprising instructions for: detecting at least one object in at least one image frame; determining at least one condition associated with the object in the at least one image frame; the at least one condition comprising a first condition of the object being determined to be an aquatic animal of interest; and, in response to the determination of the at least one condition, taking an action associated with the harvesting of aquatic animals.

The invention discloses a machine learning-based Bellamya piriformis gender prediction method and application, and belongs to the technical field of aquatic animal gender determination. According to the method, shell shape character data of the Bellamya piriformis is taken as a training sample, and a multilayer perceptron model (MLP), a principal component analysis dimensionality reduction model (PCA) and a random forest model are respectively constructed to extract gender difference characteristics of the Bellamya piriformis. The performance and prediction accuracy of the constructed MLP model are optimal, the prediction accuracy reaches 99.1%, and the recall rate and the F1 score both exceed 95%. The method breaks through the limitation of the existing bellamya piriformis sex identification method, can quickly and accurately predict the sex of the bellamya piriformis, and provides convenience for breeding of new varieties of the bellamya piriformis and screening of male and female parents in artificial propagation.

The invention discloses an ecological restoration system for mangrove forest wetlands, and relates to the technical field of ecological restoration of coastal wetlands, and the technical key points are as follows: the ecological restoration system comprises an algae-cleaning biocenosis: the number of algae is reduced through salt-tolerant algae-cleaning organisms, and the transparency of a water body is improved; the sea grass plant community comprises Sichuan fructus viticis and halophila acuminata, absorbs eutrophic substances in water through photosynthesis, and further purifies the water quality; aquatic animals comprise fishes, benthonic animals, shrimps and filter-feeding zooplanktons; the mangrove plant community is used for planting bruguiera gymnorrhiza, kandelia candel and aegiceras corniculatum in a layered manner according to tide and water depth so as to realize the functions of purifying water quality of an ecological system and providing a habitat; and the environment monitoring module is provided with tide monitoring equipment, observes tide water level change and salinity and alkalinity, and implements early warning and emergency measures under extreme climate conditions. According to the method, a multi-layer producer community system can be coupled and repaired, the eutrophication problem can be effectively solved by multi-dimensionally improving the biodiversity, and a new method is provided for coastal wetland repair.

The invention belongs to the technical field of disease prevention and treatment and aquaculture, and relates to application of acacetin in preparation of a medicine for preventing and treating white spot syndrome. The invention discloses that the acacetin has the biological activity of resisting the white spot syndrome virus for the first time, verifies that the acacetin can obviously inhibit the replication of the white spot syndrome virus in the procambarus clarkia body, and simultaneously improves the survival rate of the procambarus clarkia infected by the white spot syndrome virus. The acacetin has a good antiviral effect on the white spot syndrome virus in crustacean aquaculture, and can be applied to prevention and treatment of aquatic animal diseases caused by the white spot syndrome virus.

The invention provides an artificial breeding method of wild sal-family large yellow croaker, and belongs to the technical field of aquatic animal breeding. The invention provides an artificial breeding method of wild sal-family pseudosciaena crocea, which comprises the following steps: domesticating the wild sal-family pseudosciaena crocea, then carrying out intensive cultivation, and after the wild sal-family pseudosciaena crocea is cultivated until gonad is mature, carrying out artificial spawning induction and natural fertilization on the obtained parent fish of the sal-family pseudosciaena crocea to obtain fertilized eggs; the fertilized eggs are subjected to incubation and fry cultivation, and sal-family large yellow croaker fries are obtained; the artificial spawning method comprises the step of injecting hormone into parent fish in two steps. According to the method, favorable conditions are created for large-scale breeding of the sal-family pseudosciaena crocea, and a large number of artificially bred sal-family pseudosciaena crocea fry groups can be obtained by adopting the method.

The present invention provides a Chinese medicine composition for preventing and treating "milk disease" of Chinese mitten crab, and a preparation method and application thereof, and the present invention belongs to the technical field of Chinese medicine for aquatic animals. The Chinese medicine composition provided by the present invention comprises the following raw materials in parts by weight: 1 to 10 parts of coptis root, 1 to 10 parts of sappan wood, 1 to 10 parts of pomegranate peel, 1 to 10 parts of cloves, 1 to 10 parts of scutellaria and 1 to 10 parts of schisandra chinensis. The Chinese medicine composition provided by the present invention is for the prevention and treatment of "milk disease" of Chinese mitten crab caused by Metschnikowia bicuspidatum, and provides an efficient, safe and practical method, with an inhibition rate of more than 99.67%, low cost, simple preparation process, high raw material utilization rate, easy industrialization, and good development value and market prospects.

The invention discloses a recombinant bacterium for high-yield production of selenomethionine and a preparation method of the recombinant bacterium, and belongs to the technical field of genetic engineering. According to the invention, selenium-rich region (Enshi) soil and withered grass are taken as materials, a bacillus subtilis strain with high selenomethionine synthesis capability is separated and screened, the bacillus subtilis strain is taken as an original strain, and a recombinant strain of high-expression SeMet synthesis key gene thioredoxin reductase (TrxRB) is constructed by utilizing an expression vector pHT43, so that high-yield SeMet engineering bacteria are obtained. The bacillus subtilis rich in selenomethionine and the recombinant bacteria thereof can be used as feed additives, and can be used for supplementing SeMet and improving the feed conversion rate in the production of livestock and poultry and aquatic animals.

The invention relates to the field of aquatic feed, and provides a low-temperature adhesive type aquatic feed particle composition and a preparation method thereof. The composition is composed of puffed soybean meal, flour, fish meal, low-temperature directed enzyme, microcrystalline cellulose, modified zein nanoparticles, a protein adhesive and a modified zein ethanol water solution. The preparation method comprises the following specific steps: reacting soybean protein isolate with cross-linking enzyme to generate a protein adhesive, reacting proanthocyanidins with gallic acid to generate gallic acylated proanthocyanidins, and carrying out multi-step treatment on zein. Experimental results show that the composition has excellent water resistance and oxidation resistance, and the growth efficiency and the survival rate of aquatic animals are remarkably improved. The preparation method is simple, the cost is low, heat-sensitive substances in the feed are not damaged, and an economical, efficient and nutrient-rich feed solution is provided for aquaculture.

The invention provides a method and a device for efficiently proliferating sepiella maindroni by combining grazing and raising, and belongs to the field of aquatic animal proliferation. An efficient breeding method for sepiella maindroni integrating grazing and breeding comprises the following steps that an appropriate sea area is selected, fertilized eggs are bred in a breeding facility laid in the downstream direction of a normal fish breeding net cage, namely an empty net cage containing an artificial floating reef, the periphery of the empty net cage is provided with an algae-mussel flow blocking ecological rope, and a cover net is arranged above the empty net cage; the newly hatched cuttlefish can stay in the proliferation facility empty net cage for a long time due to the characteristics of loving reefs and drilling the net and strong space awareness, and slowly escape into natural water after gradually adapting to the proliferation environment. According to the proliferation method, cuttlefishes gradually adapt to the natural environment of the sea area, meanwhile, cuttlefish larvae are well protected enough in the fragile incubation initial stage through protection measures such as net cages, and therefore the proliferation survival rate and the maintenance efficiency are improved, and efficient proliferation is achieved.

The invention relates to the technical field of aquatic animal living body transportation, and discloses a sea urchin long-distance transportation synergistic method based on dry transportation and anhydrous volatile anesthesia, which comprises the following steps: placing sea urchin in a closed container, using a natural volatile substance as an anesthetic, loading and volatilizing through an adsorption material, and performing anhydrous anesthesia on the sea urchin; the anesthetized sea urchin is inversely placed on a fixing support with a mesh structure in a transportation container in the posture that the mouth face faces upwards and the thorns face downwards, and the sea urchin is prevented from rolling over in the transportation process through the self-locking effect of the thorns and meshes; a functional interlayer is arranged in the fixing support, and a temperature control material and a moisturizing material are placed in the functional interlayer at the same time to maintain the temperature and humidity of the transportation environment; after transportation, the sea urchins are placed in seawater containing the cactus extract for initial recovery and then transferred to flowing natural seawater to complete unified recovery, a whole-process collaborative protection chain of anhydrous anesthesia loss prevention, dry transportation steady state maintenance and rapid water phase restoration can be constructed, and the dry transportation survival rate and quality are remarkably improved.

The invention discloses marinobacter hydrocarbonoclastici SN-1 and a culture method and application thereof, the marinobacter hydrocarbonoclastici SN-1 is marinobacter hydrocarbonoclastici SN-1, is preserved in China Center for Type Culture Collection on August 7, 2024, is located in Wuhan University, Wuhan, China, and has a preservation number of CCTCC NO: M 20241755, and the preservation number of the marinobacter hydrocarbonoclastici SN-1 is CCTCC NO: M 20241755. The invention also discloses application of the marinobacter hydrocarbonoides SN-1 or the microbial inoculum in degradation of ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and / or active phosphate concentration in seawater. The seawater body is a mariculture water body, mariculture tail water or an offshore water body, the salinity of the seawater body is 10-40, and the inoculum size of the mariculture SN-1 is 2%. The marinobacter hydrocarbonoides SN-1 disclosed by the invention can remove ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, total nitrogen, active phosphate and total phosphorus in a seawater water body in an aerobic environment with relatively high salinity, has no toxic effect on aquatic animals, and can be applied to improvement and ecological restoration of a seawater water environment.

The invention discloses an application of marine streptomyces SH110 and a source compound thereof, the marine streptomyces SH110 has a preservation number of GDMCC (China General Microbiological Culture Collection Center) NO.66668, and the preservation date is July 14, 2025; the invention also discloses application of the marine streptomyces SH110-derived compound in preparation of a medicine for preventing and treating streptococcus and vibrio diseases. The marine streptomyces source compound has efficient vibrio and streptococcus resisting activity, the vibrio and streptococcus resisting activity is not reported, and the marine streptomyces source compound has new application. The marine streptomyces source compound is used for preparing the medicine for preventing and treating the vibrio and streptococcus diseases, especially for preparing the medicine for preventing and treating the vibrio and streptococcus diseases of aquaculture animals, the safety is high, and the treatment effect is good.

The invention provides an artificial propagation method of Sinilabeo wuyi, and belongs to the technical field of aquatic animal breeding. The artificial propagation method of the Sinilabeo wuyi comprises the steps of parent fish classification, parent fish rearing, artificial spawning induction, natural insemination, artificial insemination, fertilized egg incubation, fry rearing, juvenile fish rearing and the like. The oxytocic is reasonable in compatibility, the injection time of the oxytocic is accurate, the oxytocic rate, the fertilization rate and the hatching rate are increased, and damage to parent fishes is small. According to the method, feeding methods in different stages of fry breeding are also defined, the survival rate of the sinilabeo wuyi fry breeding stage is increased, and a technical support is laid for artificial breeding of the endangered species sinilabeo wuyi.

The invention belongs to the field of prevention and control of bacterial diseases of aquatic animals, and discloses an acid-resistant broad-spectrum vibrio parahaemolyticus bacteriophage LVPP58 and application of the acid-resistant broad-spectrum vibrio parahaemolyticus bacteriophage LVPP58 in vibrio disease prevention and control, and the preservation number of the bacteriophage is CCTCC M 2025322. The bacteriophage is a broad-spectrum vibrio parahaemolyticus bacteriophage, not only can split vibrio parahaemolyticus, but also can split vibrio alginolyticus, the bacteriophage LVPP58 is stable in titer at the pH of 3-11 and the temperature of 30-60 DEG C, the bacteriophage and antibiotics are combined for use, a synergistic effect can be generated, the treatment effect is enhanced, the occurrence rate of drug resistance is reduced, and a new strategy is provided for solving the problem of bacterial drug resistance.

The invention discloses a preparation with functions of promoting growth of aquatic animals and improving water quality and application of the preparation. The preparation comprises compound bacterial powder and a traditional Chinese medicine extract in a mass ratio of (2-3): (1-2), the compound bacterial powder is bacillus velezensis J-1 bacterial powder and bifidobacterium G-1 bacterial powder in a mass ratio of (2-4): (3-6), the effective viable count of the bacillus velezensis J-1 bacterial powder is 1.5 * 10 < 9 >-2.5 * 10 < 9 > CFU / g, the effective viable count of the bifidobacterium G-1 bacterial powder is 1.0 * 10 < 10 >-1.5 * 10 < 10 > CFU / g, the bacillus velezensis J-1 and the bifidobacterium G-1 are both preserved in the China Center for Type Culture Collection, and the preservation number of the bacillus velezensis J-1 bacterial powder and the bifidobacterium G-1 bacterial powder is CGMCC (China Center for Type Culture Collection). The preservation number of the bacillus velezensis J-1 is CCTCC (China Center for Type Culture Collection) No: M2021287, and the preservation number of the bifidobacterium G-1 is CCTCC No: M2024350; the traditional Chinese medicine extract is composite extract powder of rhizoma acori graminei and perilla leaves. According to the preparation, the synergistic interaction effect of the two bacteria is utilized, the effects of promoting growth of the aquatic animals and improving water quality can be remarkably improved, and meanwhile the effects of promoting growth of the aquatic animals and improving water quality can be further achieved by adding the compound traditional Chinese medicine extract of the rhizoma acori graminei and the perilla leaves.

The invention relates to the technical field of agricultural waste recycling and feed industry, in particular to a traditional Chinese medicine residue feed additive as well as a preparation method and application thereof. The preparation method comprises the following steps: drying traditional Chinese medicine residues, crushing to obtain a crushed material, mixing the crushed material with a compound enzyme, carrying out enzymolysis to obtain an enzymolysis material, mixing the enzymolysis material with lactobacillus plantarum LP-01, carrying out anaerobic fermentation to obtain a fermented material, mixing the fermented material with a yeast extract and manganese sulfate to obtain a mixture, mixing the mixture with water, extracting, filtering to obtain a filtrate, concentrating, and carrying out freeze drying to obtain a finished product. The traditional Chinese medicine residue feed additive is obtained. By adopting the traditional Chinese medicine residue feed additive provided by the invention, the diarrhea rate of weaned piglets can be reduced, the stress resistance of aquatic animals is improved, and the growth performance of poultry is improved.

The invention discloses a fresh water aquaculture tail water organism ecological purification system, which belongs to the technical field of tail water purification treatment, and comprises a purification pond module, a culture pond module and a circulation module, through the synergistic effect of the purification pond module, the culture pond module and the circulation module, deep purification of the culture tail water is achieved, the underwater forest purification unit constructed by the purification pond module comprises specific submerged plant communities, customized microbial preparations and aquatic animal communities, various pollutants in the tail water can be efficiently removed, and the water quality of the tail water is improved. The purified tail water reaches a higher water quality standard, and the pollution of the tail water to the environment is effectively reduced. Meanwhile, tail water in the culture pond module is conveyed to the purification pond module in a backflow mode through the circulation module, clean water is injected back into the culture pond module after purification, and an efficient water resource circulation system is formed; according to the system, the dependence on fresh water resources is greatly reduced, the utilization efficiency of the water resources is improved, and the breeding cost is reduced.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of litopenaeus vannamei and application of the SNP molecular marker, and belongs to the field of molecular marker-assisted breeding of aquatic animals. Specifically, after a target fragment located on the MEF2 gene of the litopenaeus vannamei is amplified through PCR (polymerase chain reaction), the genotype of the SNP molecular marker located on the MEF2 gene is identified by using a Sanger sequencing method or a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method, and three genotypes of GG, GC and CC are found. Through correlation analysis of the genotype of the SNP molecular marker and the growth traits of the litopenaeus vannamei, it is found that the growth traits of the litopenaeus vannamei with the genotype of GG are superior to those of GC and CC genotypes. Therefore, the SNP molecular marker can be used as a functional site for screening litopenaeus vannamei with excellent growth traits, and the research provides data for mining litopenaeus vannamei growth traits related molecular markers.

The invention relates to an aquatic animal-derived acellular matrix hydrogel, which comprises an acellular matrix and a non-biological auxiliary agent, and a preparation method of the aquatic animal-derived acellular matrix hydrogel. The invention further relates to application of the aquatic animal-derived acellular matrix hydrogel in preparation of a product for wound repair or application of the aquatic animal-derived acellular matrix hydrogel in preparation of a product for surgical operation.

The invention belongs to the technical field of molecular marker-assisted breeding of aquatic animals, and particularly relates to a KASP marker combination for detecting vibrio harveyi resistance of cynoglossus semilaevis and application of the KASP marker combination. The KASP marker combination disclosed by the invention is respectively positioned at the 14: 17 304461 site, the 14: 17 307045 site, the 14: 17 310738 site and the 14: 17 303501 site of the 14 # chromosome of the cynoglossus semilaevis, and the base polymorphism types of the KASP marker combination are C / T, C / G, C / T and A / G respectively. According to the invention, after genotyping is carried out on a to-be-detected individual, the to-be-detected individual is scored according to the vibrio harveyi disease resistance excellent and inferior genotypes, so that the vibrio harveyi disease resistance of the to-be-detected individual is measured; by utilizing the KASP molecular marker combination disclosed by the invention, the vibrio harveyi resistance of the cynoglossus semilaevis can be detected at low cost, high efficiency and high accuracy, and the disease-resistant breeding efficiency of the cynoglossus semilaevis is greatly improved.

The invention, which relates to the technical field of data monitoring, discloses a wireless multi-sensing data monitoring terminal system for smart rice and fishery, comprising a data setting module for setting an initial monitoring frequency; the environment monitoring module monitors the growth condition of rice and fish, soil data and the state of aquatic animals in real time, and captures the growth environment change of rice and fish in time; the data processing module is used for quantifying the change degree of the rice and fish growth environment according to the characteristic data related to the change of the rice and fish growth environment and the abnormality of the cultured object extracted by the environment monitoring module; the early warning module is used for predicting the growth environment change through a machine learning model, and automatically sending out an early warning signal when the rice and fish growth environment change is predicted to be abnormal; and the dynamic adjustment module dynamically adjusts the monitoring frequency of the monitoring equipment so as to cope with the change of the growth environment of rice and fish. According to the method, the environmental change rate is dynamically adapted by adjusting the monitoring frequency, so that the robustness, timeliness and decision accuracy of the system can be remarkably improved.

The invention relates to the technical field of aquatic animal cell culture, in particular to a rapid enrichment and separation method for gonad somatic cells of plectropomus leopardus. Comprising the following steps: selecting healthy plectropomus leopardus for anesthesia; the method comprises the following steps: taking gonad tissues, performing sterile treatment, cutting, centrifugal washing and filtering, inoculating the gonad tissues into a culture medium for primary culture to obtain gonad cells emigrated from tissue blocks, performing trypsin-EDTA digestion and centrifugal washing, and treating the gonad cells with 1 * PBS containing polysorbate; after closing, antibody incubation and washing, the marking effect is verified through a fluorescence microscope, and specific enrichment of gonad somatic cells is achieved; and filtering and purifying the enriched fluorescence-labeled cells, placing the cells under a fluorescence microscope, accurately picking fluorescence-positive cells under a fluorescence visual field, transferring the fluorescence-positive cells into a culture dish containing a complete medium, inoculating the separated fluorescence-positive cells into the optimized complete medium, and carrying out purification culture, so that the cells grow in a wall-attached manner and stable passage is realized.

The invention belongs to the technical field of aquatic animal breeding, and particularly relates to a grass carp parent salinity tolerance related molecular marker suitable for fry salinity domestication and application of the grass carp parent salinity tolerance related molecular marker in grass carp salt tolerance breeding. The nucleotide sequence of the SNP molecular marker related to the salinity tolerance character of the grass carp is as shown in SEQ ID NO: 1; a / C polymorphism exists at the 111st site in the DNA fragment shown in the nucleotide sequence, and when the genotype of the SNP molecular marker is CC or AC, the to-be-detected sample is the salinity-tolerant grass carp; when the genotype of the SNP molecular marker is AA, the to-be-detected sample is the salinity-sensitive grass carp. The SNP molecular marker closely related to the salt tolerance character is screened through a salinity tolerance test. Therefore, the molecular marker provided by the invention can be used for carrying out breeding work and detection work of the salt-tolerant grass carp.