Aquaculture composite microecological preparation and application thereof

Abstract

The invention belongs to the technical field of micro-ecology, and particularly relates to an aquaculture compound micro-ecological preparation and application thereof. The compound microecological preparation for aquaculture comprises bacillus silage YZS-M01, bacillus pettimensis YZS-M02, bacillus velezensis YZS-M03 and bacillus siamensis YZS-M04, and the total viable concentration of the compound microecological preparation is (2-5) * 10 < 9 > cfu / ml or (2-5) * 10 < 9 > cfu / g. The compound micro-ecological preparation for aquaculture has a remarkable purification effect on aquaculture water, and meanwhile, the survival rate and the yield of aquatic animals can be increased.

Description

Technical Field

[0001] The present invention belongs to the field of microecological technology and relates to a composite microecological preparation, in particular to a composite microecological preparation for aquaculture and application thereof. Background Art

[0002] In recent years, the aquaculture industry has developed rapidly and the degree of intensiveness has been continuously improved. Harmful algae and pathogens have multiplied in large numbers in many breeding ponds, causing serious damage to the breeding ecological environment and leading to frequent breeding diseases. As a new type of green and environmentally friendly product, microecological preparations play an irreplaceable role in regulating the microecological balance of animal bodies, preventing diseases, improving feed conversion rate, promoting animal health and protecting the ecological environment. At present, microecological preparations have been accepted as green feed additives and have been widely used in aquaculture. The most widely used microecological preparations on the market are probiotic preparations based on yeast, lactic acid bacteria, and bacillus, which have the functions of antagonizing intestinal pathogenic bacteria, maintaining and regulating the balance of intestinal microecology, synthesizing vitamins for the host, producing a variety of digestive enzymes, providing nutrition and preventing the invasion of pathogenic bacteria. Microecological preparations can also promote growth, convert feed, and improve the body's immunity. Bacillus products are mainly used in aquaculture, but a single bacillus sometimes fails to achieve good results, while composite microecological preparations with synergistic effects of multiple strains can play an effective role. Summary of the invention

[0003] The purpose of the present invention is to provide an aquaculture composite microecological preparation and application thereof.

[0004] The objective of the present invention is achieved through the following technical solutions: A composite microecological preparation for aquaculture, comprising Bacillus silage YZS-M01, Bacillus timonianus YZS-M02, Bacillus velez YZS-M03, and Bacillus siamense YZS-M04; The silage pit Bacillus YZS-M01, classified and named as Bacillus siralis, was deposited in the General Microbiological Center of China Microbiological Culture Collection Administration on October 16, 2020, with the deposit address at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China, and the deposit number is CGMCC No. 20899; The Oceanobacillus timonensis YZS-M02, classified and named Oceanobacillus timonensis, was deposited in the General Microbiological Center of China National Microbiological Culture Collection Administration on September 3, 2020, with the deposit address at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China, and the deposit number is CGMCC No. 20593; The Bacillus velezensis YZS-M03, classified and named Bacillus velezensis, was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on October 16, 2020, with the deposit address at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China, and the deposit number is CGMCC No. 20900; The Siamese Bacillus YZS-M04, classified and named Bacillus siamensis, was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on October 16, 2020. The deposit address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, China, and the deposit number is CGMCC No. 20901.

[0005] Furthermore, the mass ratio of solid bacterial powder or fermentation liquid of Bacillus subtilis YZS-M01, Bacillus subtilis YZS-M02, Bacillus velezensis YZS-M03, and Bacillus siamese YZS-M04 in the above-mentioned aquaculture composite microecological preparation is (2-5): (2-6): (3-8): (3-6).

[0006] Furthermore, the total live bacteria concentration in the above-mentioned aquaculture composite microecological preparation is (2-5)×10 9 cfu / ml or (2~5)×10 9 cfu / g.

[0007] Furthermore, the above-mentioned aquaculture composite microecological preparation is a solid preparation or a liquid preparation.

[0008] Furthermore, when the above-mentioned aquaculture composite microecological preparation is a solid preparation, the preparation method is: the solid bacterial powders of Bacillus subtilis YZS-M01, Bacillus thimonis YZS-M02, Bacillus velez YZS-M03, and Bacillus siamese YZS-M04 are mixed in a mass ratio of (2-5): (2-6): (3-8): (3-6) to obtain a solid mixed bacterial powder; and then the carrier and the solid mixed bacterial powder are mixed in a mass ratio of 100: (5-10) to obtain an aquaculture composite microecological preparation; wherein the carrier is obtained by mixing fish meal, soybean meal, and astragalus polysaccharide in a mass ratio of (80-100): (10-20): (1-5).

[0009] The above-mentioned aquaculture composite microecological preparation is used in the purification of aquaculture water.

[0010] The above-mentioned aquaculture composite microecological preparation is used in the preparation of an aquatic feed additive, and the aquatic feed additive has the function of improving the survival rate and yield of aquatic animals.

[0011] The significant advantages of the present invention are: The present invention provides a composite microecological preparation for aquaculture, which includes a variety of beneficial microorganisms such as Bacillus silage, Bacillus timon oceanicus, Bacillus velez and Bacillus siamese. The composite microecological preparation for aquaculture of the present invention can regulate the microecological balance of aquatic organisms, prevent diseases, promote the health of aquatic products and protect the ecological environment, and can also improve the survival rate and yield of aquatic products and reduce the mortality rate. It is a new generation of green and environmentally friendly products, and plays a very important role in protecting the aquaculture water environment and promoting the benign circulation of the aquatic ecosystem. DETAILED DESCRIPTION

[0012] In order to make the contents of the present invention easier to understand, the technical solution of the present invention is further described below in conjunction with specific implementation methods, but the present invention is not limited thereto.

[0013] The aquaculture composite microecological preparation provided by the present invention includes Bacillus siralis YZS-M01, Bacillus thimundii YZS-M02, Bacillus velez YZS-M03 and Bacillus siamese YZS-M04; wherein the Bacillus siralis YZS-M01 is classified and named Bacillus siralis, which has been deposited in the General Microbiological Center of China Microbiological Culture Collection Committee on October 16, 2020, and the deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China, and the deposit number is CGMCC No. 20899, and the 16S rDNA sequencing result is as shown in SEQ ID NO.1; the Oceanobacillus stimonensis YZS-M02, classified and named Oceanobacillus stimonensis, was deposited in the General Microbiology Center of China National Microbiological Culture Collection on September 3, 2020, with a deposit address of No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China, with a deposit number of CGMCC No.20593, and the 16S rDNA sequencing result is shown in SEQ ID NO.2; the Bacillus velezensis YZS-M03, classified and named Bacillus velezensis, was deposited in the General Microbiology Center of China National Microbiological Culture Collection on October 16, 2020, with a deposit address of No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China, with a deposit number of CGMCC No.20900, and the 16SrDNA sequencing result is shown in SEQ ID NO.3; the Siamese Bacillus YZS-M04, classified and named Bacillus siamensis (Bacillussiamensis), was deposited in the General Microbiology Center of China Culture Collection Administration on October 16, 2020, with the deposit address at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China, with the deposit number CGMCC No. 20901, and the 16S rDNA sequencing result is shown in SEQ ID NO.4.

[0014] In a preferred embodiment of the present invention, the aquaculture composite microecological preparation is a liquid preparation or a solid preparation.

[0015] When the aquaculture composite microecological preparation is a liquid preparation, the preparation is obtained by mixing the fermentation broths of the silage cellar Bacillus YZS-M01, the Timonian Bacillus YZS-M02, the Velez Bacillus YZS-M03 and the siam Bacillus YZS-M04 in a mass ratio of (2-5): (2-6): (3-8): (3-6).

[0016] When the aquaculture composite microecological preparation is a solid preparation, the solid bacterial powders of the silage pit Bacillus YZS-M01, Timon Oceanic Bacillus YZS-M02, Velez Bacillus YZS-M03, and Siamese Bacillus YZS-M04 are mixed in a mass ratio of (2-5): (2-6): (3-8): (3-6) to obtain a solid mixed bacterial powder; and then the carrier and the solid mixed bacterial powder are mixed in a mass ratio of 100: (5-10) to obtain an aquaculture composite microecological preparation; wherein the carrier is obtained by mixing fish meal, soybean meal, and astragalus polysaccharide (purchased from Jinan Qimaotang Biotechnology Co., Ltd., CAS No.: 89250-26-0) in a mass ratio of (80-100): (10-20): (1-5).

[0017] In a preferred embodiment of the present invention, the preparation method of the solid bacterial powder comprises the following steps: activating the bacterial strains and then performing continuous two-stage fermentation and concentration to obtain a concentrated bacterial solution; mixing the concentrated bacterial solutions of the silage pit Bacillus YZS-M01, Timon Oceanic Bacillus YZS-M02, Velez Bacillus YZS-M03, and Siamese Bacillus YZS-M04 with light calcium carbonate and spray-drying the mixture to obtain a solid bacterial powder.

[0018] In the present invention, the strain is inoculated into NB culture medium and cultured for 15 to 25 hours to obtain a primary seed solution; the culture temperature is preferably 25 to 35° C., and the culture speed is preferably 150 to 350 r / min.

[0019] After obtaining the primary seed solution, the present invention transfers the primary seed solution to NB liquid culture medium for 10 to 20 hours to obtain the secondary seed solution. The inoculation amount of the primary seed solution is preferably 0.5% to 1.0% (V / V), and the culture is preferably carried out in a fermenter. Sterile air is introduced during the culture process, and the fermenter ventilation is manually adjusted to adjust the speed and air flow to adjust DO (DO40%). The culture temperature is 30 to 35°C, the culture speed is 200 to 350 r / min, and the culture time is preferably 15 to 20 hours. The OD of the secondary seed solution 600 It is preferably 0.5 to 0.8.

[0020] After obtaining the secondary seed liquid, the secondary seed liquid is inoculated into the fermentation medium for fermentation culture to obtain the fermentation liquid. The inoculation amount of the secondary seed liquid is 0.5% to 1.0% (V / V), and the tank pressure during the fermentation culture is maintained at 0.05MPa; sterile air is introduced during the culture process, and the fermentation tank ventilation speed and air flow are manually adjusted to adjust DO (DO40%), the culture temperature is 30 to 35°C, the culture speed is 200 to 350r / min, and the culture time is preferably 25 to 35h. The OD of the fermentation liquid 600It is preferably 0.8 to 1.5.

[0021] After obtaining the fermented bacterial liquid, the fermented bacterial liquid is concentrated to obtain a concentrated bacterial liquid. The concentration multiple of the concentrated bacterial liquid is 5 to 10 times; the fermented bacterial liquid of the silage pit Bacillus subtilis YZS-M01, Timon Oceanic Bacillus subtilis YZS-M02, Velez Bacillus subtilis YZS-M03, and Siamese Bacillus subtilis YZS-M04 is filtered through a ceramic membrane to obtain a concentrated bacterial liquid. The present invention does not specifically limit the specific parameter settings of the concentration equipment, and conventional parameter settings can be used to achieve the above-mentioned multiple concentration.

[0022] After obtaining the fermented bacterial liquid, the fermented bacterial liquids of the silage pit Bacillus YZS-M01, Timon oceanic Bacillus YZS-M02, Velez Bacillus YZS-M03 and Siamese Bacillus YZS-M04 are filtered through a ceramic membrane to obtain a concentrated bacterial liquid, and light calcium carbonate is added to the concentrated bacterial liquid and then spray-dried to obtain a solid bacterial powder, the mass ratio of the concentrated liquid to the light calcium carbonate is 100:2-5, and the control indicators of the spray drying process are that the material inlet temperature is 150-200° C., the outlet temperature is 80-85° C., and the material is dried in the tower for 15-30S.

[0023] Example 1 The preparation steps of silage cellar Bacillus YZS-M01 bacterial solution and solid bacterial powder are as follows: S1: Primary seed solution preparation Activate the YZS-M01 strain of Bacillus silage, pick a single colony, inoculate it into 150 ml NB medium (5 g / L peptone, 10 g / L sucrose, 1 g / L yeast powder, 3 g / L beef extract, pH 6.5-7.0, sterilize at 121°C for 30 min), shake culture at 28°C and 180 r / min for 20 h to obtain a first-level seed solution; S2: Secondary seed solution preparation The first-level seed solution was inoculated into a sterile seed tank with an inoculation volume of 0.5% to 1.0% (V / V). The culture conditions were: manual ventilation speed and air flow to adjust DO (DO40%), culture temperature of 30 to 35°C, speed of 200 to 350 r / min, tank pressure of 0.05 MPa, and culture time of 15 to 20 hours. The OD of the second-level seed solution 600 The value is 0.8, at which time the strain has the strongest activity; S3: Preparation of mature fermentation liquid The secondary seed liquid is inoculated into a sterile fermentation tank at an inoculation rate of 0.5% to 1.0% (V / V). The composition of the fermentation medium is as follows (per liter): 10-20 grams of yeast extract, 25-35 grams of maltose syrup, 0.2-0.5 grams of magnesium chloride, 0.1-0.4 grams of calcium chloride, 0.1-0.4 grams of manganese chloride, and 1.0-2.5 grams of potassium dihydrogen phosphate. The culture conditions are: ventilation manually adjusts the speed and air flow to adjust DO (DO40%), the culture temperature is 30-35°C, the speed is 200-350r / min, and the tank pressure is 0.05MPa; the culture time is 30-35h, the pH rises to 7.5-8.0, and the OD 600 The value is about 1.0, and the tank time is based on the sampling microscopic examination of spores being more than 90% mature, and the fermentation is terminated to obtain the mature fermentation liquid; S4: Preparation of bacterial powder The fermented mature bacterial liquid was filtered through a ceramic membrane with a pore size of 0.15 μm, a working pressure of 0.2-0.5 MPa, and a concentration ratio of 40 times, and then the concentrated bacterial liquid was added with light calcium carbonate and mixed evenly. The mass ratio of the concentrated bacterial liquid to the light calcium carbonate was 100:4 to obtain a solid-liquid mixture, which was spray-dried. The spray-drying conditions were as follows: the material inlet temperature was 150-200°C, the outlet temperature was 80-85°C, and the material was dried in the tower for 15-30 seconds to obtain a solid bacterial powder. The moisture content of the bacterial powder was less than 5% (W / W), and the number of viable bacteria was 2×10 11 ~5×10 11 cfu / g.

[0024] The preparation method of the solid bacterial powder of Bacillus timonens YZS-M02 is basically the same as the solid bacterial powder of Bacillus silosus YZS-M01, except that the Bacillus silosus YZS-M01 in step S1 is replaced by Bacillus timonens YZS-M02.

[0025] The preparation method of the solid bacterial powder of Bacillus velez YZS-M03 is basically the same as the preparation method of the solid bacterial powder of Bacillus velez YZS-M01, except that the Bacillus velez YZS-M01 in step S1 is replaced by Bacillus velez YZS-M03.

[0026] The preparation method of siam Bacillus subtilis YZS-M04 solid bacterial powder is basically the same as the preparation method of the above-mentioned silo Bacillus subtilis YZS-M01 solid bacterial powder, except that the silo Bacillus subtilis YZS-M01 in step S1 is replaced by siam Bacillus subtilis YZS-M04.

[0027] Example 2 The solid bacterial powder of Bacillus silage YZS-M01, the solid bacterial powder of Bacillus Timonii YZS-M02, the solid bacterial powder of Bacillus Velezii YZS-M03, and the solid bacterial powder of Bacillus siamese YZS-M04 prepared in Example 1 were mixed in a mass ratio of 5:6:8:6 to obtain a solid mixed bacterial powder; and the carrier and the solid mixed bacterial powder were mixed in a mass ratio of 10:1 to obtain a solid preparation of aquaculture composite microecological preparation. The total viable bacteria concentration in the solid preparation of aquaculture composite microecological preparation is 5×10 9 cfu / g; the carrier is obtained by mixing fish meal, soybean meal and astragalus polysaccharide in a mass ratio of 90:15:1.

[0028] Example 3 The solid bacterial powder of Bacillus silage YZS-M01, the solid bacterial powder of Bacillus Timonii YZS-M02, the solid bacterial powder of Bacillus Velezii YZS-M03, and the solid bacterial powder of Bacillus siamese YZS-M04 prepared in Example 1 were mixed in a mass ratio of 2:2:3:3 to obtain a solid mixed bacterial powder; and the carrier and the solid mixed bacterial powder were mixed in a mass ratio of 100:5 to obtain a solid preparation of aquaculture composite microecological preparation. The total viable bacteria concentration in the solid preparation of aquaculture composite microecological preparation is 2×10 9 cfu / g; the carrier is obtained by mixing fish meal, soybean meal and astragalus polysaccharide in a mass ratio of 80:10:3.

[0029] Example 4 The solid bacterial powder of Bacillus silage YZS-M01, the solid bacterial powder of Bacillus Timonii YZS-M02, the solid bacterial powder of Bacillus Velezii YZS-M03, and the solid bacterial powder of Bacillus siamese YZS-M04 prepared in Example 1 were mixed in a mass ratio of 4:4:6:5 to obtain a solid mixed bacterial powder; and the carrier and the solid mixed bacterial powder were mixed in a mass ratio of 100:8 to obtain a solid preparation of aquaculture composite microecological preparation. The total viable bacteria concentration in the solid preparation of aquaculture composite microecological preparation is 3×10 9 cfu / g; the carrier is obtained by mixing fish meal, soybean meal and astragalus polysaccharide in a mass ratio of 100:20:5.

[0030] Example 5 Application of the solid preparation of aquaculture composite microecological preparation prepared in Example 2 to purification of water for cultivation of whiteleg shrimp A whiteleg shrimp cultivation pond in a factory-based indoor winter shed model was selected, and the whiteleg shrimp in the cultivation pond were young shrimps of about one month old. Test pool: Apply the aquaculture composite microecological preparation solid preparation prepared in Example 2; Control pool 1: Apply the silage pit Bacillus YZS-M01 solid bacterial powder with the same total live bacteria concentration as the aquaculture composite microecological preparation solid preparation prepared in Example 2; Control pool 2: Apply the Timon Ocean Bacillus YZS-M02 solid bacterial powder with the same total live bacteria concentration as the aquaculture composite microecological preparation solid preparation prepared in Example 2; Control pool 3: Apply the Bacillus Velez YZS-M03 solid bacterial powder with the same total live bacteria concentration as the aquaculture composite microecological preparation solid preparation prepared in Example 2; Control pool 4: Apply the Siamese Bacillus YZS-M04 solid bacterial powder with the same total live bacteria concentration as the aquaculture composite microecological preparation solid preparation prepared in Example 2; Control pool 5: Do not apply any aquaculture composite microecological preparation solid preparation or solid bacterial powder or other similar products. Test pool and control pools 1 to 4: Apply 20 grams each day for 2 consecutive days. The composite microecological preparation and single-strain solid powder are used in the morning on sunny days, and are activated before each application: the composite microecological preparation products and single-strain solid powder are placed in containers such as buckets, added to the aquaculture water body, and placed in an aeration head or agitator to facilitate rapid activation, and each activation is 2 hours. After the composite microecological preparation and single-strain solid powder are activated, they are evenly sprinkled in the corresponding test ponds and control ponds 1 to 4, and the aerator is turned on for 2 hours after the sprinkling is completed. Observe the changes in water color regularly, and reduce the water change ratio according to the water quality. During the test, no other biological, chemical, disinfectant or other products that affect the results are added.

[0031] The results showed (Table 1) that the water transparency of the test pool treated with the solid preparation of the aquaculture composite probiotic preparation gradually increased over time. On the second day after the application of the solid preparation of the aquaculture composite probiotic preparation, the water exchange volume of the test pool decreased by 50%, and the total water exchange volume in the following three days decreased by 80%, which was significantly different from the control pools 1 to 4 in which the water exchange volume decreased by about 30% after the application of the corresponding single-species solid bacterial powder. The water color of the test pool treated with the solid preparation of the aquaculture composite probiotic preparation was significantly different from the light brown water color of the control pools 1 to 4 treated with the single-species solid bacterial powder.

[0032] Table 1 The effect of compound microecological preparation on the purification of water for Penaeus vannamei culture Example 6 Effect of the solid preparation of aquaculture composite microecological preparation prepared in Example 3 on the survival rate and yield of white shrimp farming. A white shrimp farming pond (high-level pond) was selected, and two treatments were set, namely: test pond: the solid preparation of aquaculture composite microecological preparation prepared in Example 3 was applied; control pond: no solid preparation of aquaculture composite microecological preparation or other similar products were applied. The average weight of the seedlings in the test pond and the control pond was 4.6 grams / tail, the breeding area was 0.5 mu / pond, and the breeding days were 90 days. The solid preparation of aquaculture composite microecological preparation was mixed with feed for use, and the solid preparation of aquaculture composite microecological preparation was activated before each application: the solid preparation of aquaculture composite microecological preparation was placed in a container such as a bucket, added to the breeding water body, and placed in an aeration head or stirred to facilitate rapid activation, and each activation required about 3 hours. After the solid preparation of aquaculture composite microecological preparation is activated, it is mixed with the feed in proportion and fed evenly. In the early stage of aquaculture (stocking - 60 days of aquaculture), the solid preparation of aquaculture compound microecological preparations and feed were mixed at a mass ratio of 5:100; in the late stage of aquaculture (60 days of aquaculture - fishing), the solid preparation of aquaculture compound microecological preparations and feed were mixed at a mass ratio of 8:100. Water transparency, ammonia nitrogen and nitrite nitrogen and other indicators were measured regularly. No other biological, chemical, disinfectant and other products that may affect the results were added during the test.

[0033] The results showed (Table 2) that the water transparency of the test ponds where the solid preparations of the aquaculture composite microecological preparations were applied increased by 42.42%, and the concentrations of ammonia nitrogen and nitrite nitrogen in the water were significantly reduced. The survival rate of white shrimp after applying the solid preparations of the aquaculture composite microecological preparations was 96%, which was 21.52% higher than that of the control shrimp ponds; the qualified rate of normal shrimp bodies in the test ponds after applying the solid preparations of the aquaculture composite microecological preparations was 75%, which was 33.93% higher than that of the control ponds; the yield per mu of shrimp ponds increased by 23%.

[0034] Table 2 Effects of compound microecological preparations on water purification for Penaeus vannamei aquaculture Table 3 Effects of compound microecological preparations on the survival rate and yield of Penaeus vannamei culture The above description is only a preferred embodiment of the present invention. All equivalent changes and modifications made according to the scope of the patent application of the present invention should fall within the scope of the present invention.

Claims

1. A composite microecological preparation for aquaculture, characterized by: Including silage pit Bacillus YZS-M01, Timon Oceanic Bacillus YZS-M02, Velez Bacillus YZS-M03, Siamese Bacillus YZS-M04; The silage cellar Bacillus YZS-M01 is classified and named as silage cellar Bacillus ( Bacillus siralis ), which was deposited in the General Microbiology Center of China Microorganism Culture Collection Committee on October 16, 2020, with the deposit address at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China, and the deposit number is CGMCC No. 20899; The Timon Oceanic Bacillus YZS-M02 is classified and named Timon Oceanic Bacillus ( Oceanobacillus timonensis ), which was deposited in the General Microbiology Center of China Microorganism Culture Collection Committee on September 3, 2020, with the deposit address at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China, and the deposit number is CGMCC No.20593; The Velez Bacillus YZS-M03 is classified and named Velez Bacillus ( Bacillus velezensis ), which was deposited in the General Microbiology Center of China Microorganism Culture Collection Committee on October 16, 2020, with the deposit address at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China, and the deposit number is CGMCC No.20900; The Siamese Bacillus YZS-M04 is classified and named Siamese Bacillus ( Bacillus siamensis ), which was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on October 16, 2020. The deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China, and the deposit number is CGMCC No.20901.

2. The aquaculture composite microecological preparation according to claim 1, characterized in that: The mass ratio of solid bacterial powder or fermentation liquid of Bacillus silage YZS-M01, Bacillus timonensis YZS-M02, Bacillus velezensis YZS-M03 and Bacillus siamese YZS-M04 in the aquaculture composite microecological preparation is (2-5): (2-6): (3-8): (3-6).

3. The aquaculture composite microecological preparation according to claim 1, characterized in that: The total live bacteria concentration in the aquaculture composite microecological preparation is 2×10 9 ~5×10 9 cfu / ml or 2×10 9 ~5×10 9 cfu / g.

4. The aquaculture composite microecological preparation according to claim 1, characterized in that: The aquaculture composite microecological preparation is a solid preparation or a liquid preparation.

5. The aquaculture composite microecological preparation according to claim 4, characterized in that: When the aquaculture composite microecological preparation is a solid preparation, the preparation method is: mixing solid bacterial powders of Bacillus silage YZS-M01, Bacillus timon oceanicus YZS-M02, Bacillus velez YZS-M03 and Bacillus siamese YZS-M04 in a mass ratio of 2-5:2-6:3-8:3-6 to obtain solid mixed bacterial powder; then mixing a carrier with the solid mixed bacterial powder in a mass ratio of 100:5-10 to obtain the aquaculture composite microecological preparation; wherein the carrier is obtained by mixing fish meal, soybean meal and astragalus polysaccharide in a mass ratio of 80-100:10-20:1-5.

6. Use of the aquaculture composite microecological preparation as claimed in claim 1 in purification of aquaculture water.

7. The use of the aquaculture composite microecological preparation according to claim 1 in the preparation of aquatic feed additives, characterized in that: The aquatic feed additive has the function of improving the survival rate and yield of aquatic animals.

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