235 results about "Antimicrobial peptides" patented technology

The invention discloses an application of an antibacterial peptide and an application of the antibacterial peptide in preparation of antibacterial drugs for treating carbapenem-resistant pseudomonas aeruginosa, klebsiella pneumoniae (ATCC700603), escherichia coli (ATCC25922), staphylococcus aureus and methicillin-resistant staphylococcus aureus, the antibacterial peptide is characterized in that the sequence is w-r-r-w-k-r-w-w-r-r, and the sequence is shown in the description. All amino acids are D-type amino acids; the antibacterial peptide can be used as an antibacterial substance in wash supplies, food preservative additives, medical consumable antibacterial agents and cosmetics, can be synthesized through an Fmoc solid-phase chemical method, and is low in synthesis difficulty and good in in-vivo antibacterial activity. Particularly, the antibacterial peptide has broad-spectrum killing activity against carbapenem pseudomonas aeruginosa, klebsiella pneumoniae (ATCC700603), escherichia coli (ATCC25922), staphylococcus aureus and methicillin-resistant staphylococcus aureus, the cell survival rate of the antibacterial peptide within the range of medium and low concentration (2 mu g / mL-32 mu g / mL) reaches half or more, the toxicity is small, the toxicity is almost avoided, the operability is high, and the antibacterial peptide can be widely applied to the field of medical application. The cost is low.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an LL-37 derived antibacterial peptide and application thereof. According to the invention, 17-29aa of the LL-37 antibacterial peptide is used as a core fragment and is connected with an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2 through an amido bond, the antibacterial activity of the obtained antibacterial peptide is obviously higher than that of the LL-37 antibacterial peptide, and the antibacterial peptide shows an obvious bactericidal effect on clinical drug-resistant bacteria such as acinetobacter baumannii, pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, klebsiella pneumoniae and the like; meanwhile, the cytotoxicity and hemolytic activity are weak, drug resistance is not prone to being generated, bacteria can be rapidly killed in vivo through multiple mechanisms, no obvious cytotoxicity exists, the bacterium load in tissue is remarkably reduced, and the lifetime of a bacterium-infected mouse is prolonged.

The invention discloses an antibacterial small peptide CsCAPE9 and application of the antibacterial small peptide CsCAPE9 in prevention and control of citrus huanglongbing. The amino acid sequence of the antibacterial small peptide CsCAPE9 is as shown in SEQ ID NO.1; the antibacterial small peptide CsCAPE9 provided by the invention is a small peptide synthesized in a plant body, and the antibacterial small peptide CsCAPE9 can activate biosynthesis of salicylic acid and induce systematic defense reaction of citrus. The antibacterial small peptide CsCAPE9 has a good inhibition effect on pathogenic bacteria of citrus huanglongbing in greenhouses and fields, and has a wide application prospect in comprehensive prevention and control of the huanglongbing.

The invention provides a polyvinyl alcohol / chitosan composite compression hemostatic sponge with an antibacterial function and a preparation method thereof, and belongs to the technical field of medical materials. The polyvinyl alcohol / chitosan composite compression hemostatic sponge with the antibacterial function, provided by the invention, comprises a dual-network hydrogel structure formed by carboxymethyl chitosan and aldehyde polyvinyl alcohol; the double-network hydrogel structure has high stability and fixity, so that the composite compression hemostatic sponge has excellent stability. Raw materials used in the invention comprise carboxymethyl chitosan, formylated polyvinyl alcohol and antibacterial peptide, and the three raw materials are connected through covalent bonds and ionic bonds to form a dual-network hydrogel structure. By introducing gallic acid and copper chloride, a metal-organic network structure can be formed, the mechanical stability of the composite compression hemostatic sponge can be further improved, and the antibacterial performance of the composite compression hemostatic sponge can be further improved.

This invention provides an antimicrobial peptide spray, its preparation method, and its application in the prevention and treatment of peristomal skin infections. The spray comprises, by weight percentage, 0.05%-0.5% of a complex antimicrobial peptide, 0.1%-5% of a moisturizing and repairing film-forming matrix, 0.05%-0.2% of a buffer, 0.8%-1.2% of an isotonic regulator, 0.01%-0.5% of a stabilizer, and 0.005%-0.05% of a metal ion chelating agent, with the balance being water for injection. The complex antimicrobial peptide is composed of bovine lactoferrin antimicrobial peptide, sericin oligopeptide, and buckwheat-derived anti-inflammatory active peptide in a weight ratio of (3-5):(2-3):1. This spray possesses four core functions: broad-spectrum antibacterial activity, inhibition of bacterial biofilm, anti-inflammatory and soothing effects, and skin barrier repair. It is less likely to induce bacterial resistance, does not irritate damaged skin around the stoma, and can effectively prevent and treat peristomal skin infections.

This invention discloses an antimicrobial peptide PA targeting multidrug-resistant Gram-negative bacteria and its application. The amino acid sequence of the antimicrobial peptide PA is shown in SEQ ID NO.1. The antimicrobial peptide PA provided by this invention has a significant antibacterial effect against multidrug-resistant Gram-negative bacteria, and has a rapid bactericidal onset, no hemolysis or cytotoxicity, does not cause nephrotoxicity after treatment, and has good biosafety. It can be used for the treatment of bacterial infections and can also be applied to other scenarios that require bactericidal or antibacterial growth inhibition.

The invention discloses an antibacterial peptide induced by sulfate reducing bacteria and application of the antibacterial peptide, and belongs to the technical field of marine corrosion protection. The amino acid sequence of the SRB-induced antibacterial peptide is VAHKRLVVGTR, and the SRB-induced antibacterial peptide has efficient antibacterial activity on corrosive bacteria such as SRB and has the advantages of being environmentally friendly and not prone to inducing the bacteria to generate drug resistance, and the problems that an existing antibacterial peptide is insufficient in corrosive bacteria specificity, poor in environmental stability and the like are solved; meanwhile, peptide chains are short, synthesis is easy, and the production cost is low; the compound corrosion inhibition bactericide formed by combining the compound with a chemical bactericide can significantly improve the antibacterial and antiseptic effects, saves the dosage of the chemical bactericide, effectively solves the problems of bacterial drug resistance, high cost, environmental pollution and other pain points caused by abuse of the existing bactericide, and is suitable for the fields of marine microbial corrosion protection and the like.

The invention relates to an antimicrobial peptide consisting of the amino acid sequence: RX1WILX2WLRTWX3X4 wherein X1, X2, X3 and X4 are independently selected from K and R, and wherein each amino acid is independently in the L or D configuration, or a salt or solvate thereof. The use of said antimicrobial peptide in the treatment of an infection caused by Gram negative bacteria, Gram positive bacteria, fungi, and / or yeasts, as well as the use of said peptide as an antimicrobial agent for the prevention of contamination of an article or a living tissue by and / or for the decontamination of an article or a living tissue from Gram negative bacteria, Gram positive bacteria, fungi, and / or yeasts are further aspects of the invention.

This invention discloses a *Bacillus laterosporus* strain for the stable production of nonribosomal cyclic lipolipeptide and its construction method. The M1A gene in the Bogorol biosynthetic gene cluster is knocked out using CRISPR-Cas9 technology to block the Bogorol pathway, which competes with Brevicidine for substrates. Subsequently, the pMCCas9-S48 plasmid, which integrates S8 and S41 sgRNAs and their homologous arms as repair templates, is used to simultaneously knock out S8 and S41 family peptidase genes, preventing the expression of extracellular degradation enzymes. This invention also provides the application of the above strain in the preparation of nonribosomal cyclic lipolipeptide of Brevicidine and a method for preparing the nonribosomal cyclic lipolipeptide. This strain, through reducing metabolic competition and preventing product degradation, exhibits rapid cell growth, stable production of Brevicidine, and significantly reduced degradation rate, resulting in a 66.13% increase in fermentation yield compared to the unmodified strain. This invention solves the problems of degradation and unstable yield in the production of Brevicidine, provides an effective technical approach for the industrial production of Brevicidine, and provides important technical support for the development and application of novel antimicrobial peptide drugs.

This invention belongs to the field of antimicrobial peptide technology, and particularly relates to a broad-spectrum antimicrobial peptide with low hemolytic activity and its applications, as well as a broad-spectrum antibacterial drug. The antimicrobial peptide is named AY6, and its amino acid sequence is shown in SEQ ID NO.1. Antimicrobial peptide AY6 exhibits significant antibacterial effects against both Gram-positive and Gram-negative bacteria, with a bactericidal rate far exceeding that of traditional antibiotics, enabling highly effective responses to bacterial infections. AY6 has low hemolytic activity and good biocompatibility, making it suitable for medical anti-infective applications. The antimicrobial peptide provided in this application has a short sequence, is easy to synthesize chemically, can save on large-scale production costs, and has broad application prospects.

The invention relates to the technical field of pickling of meat products, and discloses an internal and external coloring pickling process of meat products, which comprises the following steps: firstly collecting blood of a meat product to be pickled, separating blood cells from plasma by adopting a centrifugal separation method, and then carrying out hemolysis treatment on the blood cells by utilizing an acidic buffer solution. According to the internal and external coloring and pickling process of the meat product, processing equipment, a natural coloring agent, a natural preservative and a natural pickling agent are adopted, and when hemoglobin pigment is extracted, centrifugal separation and ion exchange chromatography technologies are applied, so that high purity of the pigment is ensured, and a basis is provided for stable internal coloring. In the rolling and pickling link, composite natural microorganism fermentation pickling liquid is used, components of the composite natural microorganism fermentation pickling liquid are carefully screened and cultured, microorganisms such as lactic acid bacteria and the like and metabolite antibacterial polypeptides thereof not only can inhibit growth and reproduction of harmful microorganisms, but also can enable meat products to uniformly absorb components such as salt and spices in the pickling process, so that the meat products are not prone to damage. And the quality difference caused by non-uniform pickling is effectively avoided.

The application provides a composite antibacterial peptide preparation, a preparation method and application. The composite antibacterial peptide preparation comprises a first defensin, a second defensin, a first cecropin and a second cecropin. The amino acid sequence of the first defensin is shown in SEQ ID NO:1, the amino acid sequence of the second defensin is shown in SEQ ID NO:2, the amino acid sequence of the first cecropin is shown in SEQ ID NO:3, and the amino acid sequence of the second cecropin is shown in SEQ ID NO:4. Compared with a single antibacterial peptide, the composite antibacterial peptide preparation has good stability, and shows good inhibitory effect on various gram-positive bacteria, gram-negative bacteria and fungi.

The invention discloses a chemotactic response type self-assembly antibacterial peptide and a preparation method and application thereof, the antibacterial peptide comprises: (a) a targeting module, the amino acid sequence of which is selected from KPRSVS or a variant thereof, GPLVPRG or a variant thereof, and GPLGVRG or a variant thereof; (b) a self-assembly module which is composed of hydrophobic residues and hydrophilic residues which are alternately arranged and forms beta-folding driving nanofibers; (c) an inflammation response module which is selected from a histidine enrichment region, an ROS sensitive group or an MMP protease cleavage site and is used for responding to an inflammation microenvironment and enhancing target site enrichment; (d) a stability enhancing module comprising at least one of D-type amino acid modification, N-terminal PEG4 connection or cyclization structures. The antibacterial peptide disclosed by the invention has chemotactic effect on an inflammatory microenvironment, can accurately identify gram-negative bacteria, is triggered in the inflammatory environment, and plays an accurate bactericidal effect on various pathogenic bacteria.

An antimicrobial peptide against Bacillus cereus, a preparation method therefor and a use thereof. The antimicrobial peptide against bacillus cereus has the general amino acid sequence formula of [KR]GG[KR]XC[FWY]C[KR]X[KR][FWY]C[LIAV]CXG[KR][KR], wherein the antimicrobial peptide XWC26 has a strong antibacterial activity against Bacillus cereus, efficiently inhibiting the spore germination of Bacillus cereus, and effectively controlling Bacillus cereus contamination in food. The antimicrobial peptide against bacillus cereus has the amino acid sequence of KGGKLCWCKNKWCICLGKR, with a molecular weight of 2224.79 Da, an isoelectric point of 9.70, and a net charge of 6. The antimicrobial peptide also has the advantages of high stability, reduced risk of drug resistance induction, high synthesis efficiency, low production costs, etc.

According to the present invention, a technique which enables the production of an antimicrobial peptide Persulcatusin at reduced cost has been developed. A plant cell into which a polynucleotide sequence that encodes a gene for a protease to which a signal peptide localized in an intercellular organelle is added and a polynucleotide sequence that encodes a Persulcatusin fusion protein to which a signal peptide localized in an intracellular organelle different from the aforementioned intracellular organelle is added are introduced is produced, wherein, in the Persulcatusin fusion protein, a sequence that is cleavable with the protease is disposed between Persulcatusin and a protein that fuses to the Persulcatusin. Thus, Persulcatusin, which can serve as a therapeutic agent for bovine mastitis, can be produced at low cost.

This invention provides the application of Hepcidin-1 (Ms-Hepcidin-1) and its synthetic polypeptide in the preparation of antiviral drugs for largemouth bass. The invention reveals that the Ms-Hepcidin-1 synthetic polypeptide exhibits significant antiviral activity and is a novel antimicrobial peptide with the function of inhibiting fish viruses. Both the Ms-Hepcidin-1 synthetic polypeptide and its recombinant eukaryotic expression vector can significantly reduce the transcriptional levels of MCP, MMP, and DNMT genes and the MCP protein level of LMBV, inhibit LMBV replication, and reduce viral titers, demonstrating significant antiviral activity against fish viruses. Furthermore, the polypeptide synthesized from Ms-Hepcidin-1 is simple to prepare, low in cost, safe to use, and easy to store. As a novel antiviral functional gene product, it can be applied to the preparation of antiviral drugs for fish, which is of great significance for the prevention and control of iridovirus disease in fish.

This invention discloses an antimicrobial peptide SI-20 and its application in the preparation of antimicrobial drugs, belonging to the field of biomedical technology. The amino acid sequence of the antimicrobial peptide SI-20 is shown in SEQ ID NO.2. The antimicrobial peptide SI-20 provided by this invention has a highly efficient killing ability against a variety of Gram-negative / positive bacteria, effectively retaining antimicrobial activity while significantly reducing cytotoxicity and improving therapeutic efficacy. Simultaneously, this antimicrobial peptide SI-20 exhibits good stability in serum environments and under heat treatment, strongly inhibits biofilm formation, and possesses rapid and efficient antimicrobial properties, providing a highly promising candidate resource for the development of highly effective and low-toxicity antimicrobial drugs, with broad application potential.

The present invention relates to a novel peptide derived from Hirudo nipponia, which has antimicrobial activity, antibiofilm activity, antibiotic sensitivity–enhancing activity, and anti-inflammatory activity. With antimicrobial activity, antibiofilm activity, antibiotic sensitivity–enhancing activity, and anti-inflammatory activity, the novel peptide derived from Hirudo nipponia according to the present invention can be advantageously applied as a material for various purposes in the pharmaceutical field, food field, cosmetic field, and agriculture and livestock industries. In addition, the novel antimicrobial peptide derived from Hirudo nipponia according to the present invention increases the sensitivity of antibiotics to multidrug-resistant bacteria, thereby markedly enhancing antimicrobial activity against multidrug-resistant bacteria when used in combination with antibiotics. Accordingly, a composition of the present invention comprising the peptide as an active ingredient can be advantageously used as a pharmaceutical composition for enhancing antibiotic sensitivity. In particular, the novel peptide of the present invention has very low hemolytic activity and cytotoxicity, and thus is safe for the human body. Moreover, the novel peptide of the present invention not only has excellent activity even under a high salt concentration, but also maintains activity even in a serum environment, thereby having high stability when applied in vivo.

This invention discloses an antimicrobial peptide cAMP-1 derived from the intestinal microorganisms of wild rodents and its applications, belonging to the field of biotechnology. The amino acid sequence of the antimicrobial peptide cAMP-1 is shown in SEQ ID NO:1. The antimicrobial peptide cAMP-1 exhibits highly efficient, rapid, and safe antimicrobial activity against Streptococcus suis type II. It significantly inhibits bacterial growth even at low concentrations, with a minimum inhibitory concentration (MIC) of 16 µg / mL. Time-kinetic kinetics results show that the antimicrobial peptide can kill all Streptococcus suis type II within 30 min and 15 min at concentrations of 2×MIC and 4×MIC, respectively. Furthermore, within several times the effective concentration range, the peptide exhibits almost no hemolytic activity and low cytotoxicity within a safe dosage range, demonstrating its promising application as a novel anti-infective candidate drug.

This invention discloses the antimicrobial peptide PJX13 and its application in the control of citrus canker. The sequence of the antimicrobial peptide PJX13 is AcKKFWKLRRWVKWIGNH2. It can effectively inhibit and kill *Xanthomonas citrus* by disrupting the cell membrane integrity of pathogens, leading to leakage of intracellular substances. It effectively inhibits the expansion of lesions on both detached citrus leaves and living plants, and can be formulated into an antimicrobial product against *Xanthomonas citrus* for the control of citrus canker. Furthermore, the antimicrobial peptide PJX13 can also inhibit *Escherichia coli*, *Staphylococcus aureus*, and *Bacillus oryzae*, and can be used to prepare broad-spectrum antimicrobial products. This invention is beneficial for the control of citrus canker, and has advantages such as being environmentally friendly and unlikely to induce drug resistance.

This invention provides a multi-component resource utilization process for black soldier fly larvae larvae slurry, relating to the field of insect resource utilization technology. The process sequentially includes raw material pretreatment, compound enzymatic hydrolysis, lactic acid bacteria fermentation, stratified separation, and targeted treatment of each component. The compound enzymatic hydrolysis uses a combined enzyme system of trypsin and papain, and the lactic acid bacteria fermentation uses *Lactobacillus plantarum*. The fermentation parameters are: carbon-to-nitrogen ratio of 20:1, material-to-liquid ratio of 4:1 (w / v), pH of 6.5, fermentation temperature of 37°C, and fermentation time of 24 hours. This process achieves the synergistic recovery and high-value utilization of all components of black soldier fly larvae. A single process can simultaneously produce four types of products: insect oil, water-soluble fertilizer, antimicrobial peptide additives, and antibiotic-free aquatic feed ingredients, significantly improving resource utilization and industrial added value. The entire process involves no organic solvent addition, employs low-temperature enzyme inactivation to reduce energy consumption, is environmentally friendly and energy-saving, and avoids antibiotic resistance issues.

This invention relates to an endogenous antimicrobial peptide from bovine milk and its applications. The antimicrobial polypeptide compound VAVALARPKHPIK is derived from endogenously enzymatically hydrolyzed bovine casein, with the amino acid sequence Val-Ala-Val-Ala-Leu-Ala-Arg-Pro-Lys-His-Pro-Ile-Lys. It exhibits broad inhibitory activity against both Gram-positive and Gram-negative bacteria, including *Porphyromonas gingivalis*, *Escherichia coli*, and *Staphylococcus aureus*. This antimicrobial peptide shows promising applications as a health supplement and / or medical device or pharmaceutical for the prevention and / or reduction of infectious diseases, as a food antimicrobial agent, in novel functional foods, pet food and animal feed additives, and in daily chemical products.

The invention relates to the technical field of artificial dermis, in particular to a preparation method and application of artificial dermis based on bionic acellular matrix. The artificial dermis comprises a bionic acellular matrix component A and an auxiliary material component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, mother cell protein and glucan; the component B comprises antibacterial peptide, antibiotics and a high-molecular polymer material; the mass ratio of the component A to the component B is (4-6): (1-4). The preparation method comprises the following steps: respectively preparing procedure mother liquor of the component A and the component B, preparing hydrogel with a double-layer gradient structure through step-by-step crosslinking, and then carrying out freeze drying and sterilization treatment. The artificial dermis disclosed by the invention has good biocompatibility and antibacterial property, can effectively promote wound healing, is suitable for repairing acute and chronic wounds of four limbs, faces and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

This invention provides modified antimicrobial peptides, belonging to the field of antimicrobial peptide technology. The modified antimicrobial peptides include at least one of a first, second, third, fourth, fifth, and sixth antimicrobial peptide; the amino acid sequences of the first, second, third, fourth, fifth, and sixth antimicrobial peptides are shown sequentially as SEQ ID NO.1 to SEQ ID NO.6. Based on prevotellin-2, the modified antimicrobial peptides were obtained by reducing negatively charged amino acids, increasing positively charged amino acids, introducing hydrophobic amino acids, adjusting or deleting amino acid sequences, and optimizing the peptide structure. In vitro minimum inhibitory concentration (MIC) determination showed that, compared with the initial peptide, the modified antimicrobial peptides exhibited significantly enhanced antimicrobial activity against bacteria and fungi, and also showed significant bactericidal activity against clinically resistant bacteria.

Antimicrobial peptides of general formula X0X1X2C X3X4X5CX6X7X8X9CYX10X11CX12X13 are provided. Also provided are certain formulations containing these peptides and methods of using these peptides for treating skin infections in an animal in need thereof.

An antimicrobial peptide having an amino acid sequence selected from SEQ ID NOs: 2-12 is provided. The antimicrobial peptide has antimicrobial activity against Gram-negative bacteria, Campylobacter jejuni, Escherichia coli, Salmonella enterica subsp. enterica serovar typhimurium, Salmonella enterica subsp. enterica serovar choleraesuis, Klebsiella pneumonica, and / or Enterobacter cloacae. A composition, pharmaceutical composition, food additive, cosmetic composition, or hygine product having an antimicrobial peptide including an amino acid sequence selected from SEQ ID NOs: 2-12 is also provided. A method of treating an infectious disease caused by bacteria including a step of administering a pharmaceutical composition having the antimicrobial peptide as an active ingredient is also provided.

This invention discloses a strain of *Pediococcus pentosaceus* RL-10 and its application in bio-acidification rice soaking and bacterial cellulose production, belonging to the field of microbial technology. The preservation number of *Pediococcus pentosaceus* RL-10 is CCTCC NO: M 20252428. This invention is the first to use *Pediococcus pentosaceus* as a strain for bio-acidification rice soaking. Using rice soaking water as a production substrate, *Acetobacter Hansenulatus* and strain RL-10 are co-cultured in situ to produce bacterial cellulose loaded with antimicrobial peptides. Under certain process conditions, acidification of rice soaking by strain RL-10 can rapidly increase acidity and shorten soaking time. The bacterial cellulose production process of this invention is simple and low-cost; verification shows that the bacterial cellulose prepared by the method of this invention has high yield and good antibacterial activity, meeting the requirements of large-scale industrial production.

The invention belongs to the technical field of radiopharmaceutical chemistry and clinical nuclear medicine, and particularly relates to an antibacterial peptide UBI 29-41 derivative containing proline as well as a preparation method and application thereof. The proline-containing antibacterial peptide UBI 29-41 derivative is labeled by radionuclide to obtain a radioactive preparation, and the radioactive preparation has high uptake in a bacterial infection part, has a good infection / non-target ratio, and can be specifically combined with bacteria. The radioactive preparation provided by the invention can be used as a novel radioactive drug for diagnosis of bacterial infection, and has good clinical popularization and application prospects and value.

This invention relates to Bacillus vesiculosus antimicrobial peptides and their application in food preservation, belonging to the field of biotechnology. The amino acid sequence of the antimicrobial peptide is shown in SEQ ID NO: 1. The antimicrobial peptide provided by this invention is a novel antimicrobial peptide in structure, enriching the antimicrobial peptide database; this antimicrobial peptide can effectively inhibit the normal growth of Fusarium oxysporum, has a broad antimicrobial spectrum, and has potential development and application prospects; this antimicrobial peptide can effectively extend the refrigerated shelf life of dragon fruit, and has good application prospects in fruit preservation.

The invention belongs to the technical field of biological medicines, and particularly relates to application of an anti-HPV16 antimicrobial peptide in preparation of an antiviral medicine. The amino acid sequence of the antimicrobial peptide Perceptide-TJ-2 is as shown in SEQ ID NO. 1, and the antimicrobial peptide Perceptide-TJ-2 is an amino acid sequence shown in SEQ ID NO. The invention evaluates the antiviral activity of the antimicrobial peptide Perceptide-TJ-2 on HPV16 in vitro, the antimicrobial peptide Perceptide-TJ-2 can effectively inhibit the infection of HPV16 on cells at a lower concentration, the drug selection index reaches 64.64, and the antimicrobial peptide Perceptide-TJ-2 has a certain application prospect and provides a new possibility for the research and development of anti-HPV treatment drugs.