37 results about "Membrane permeability" patented technology

The invention discloses a preparation method of a high-activity exosome hair growth promoter, which comprises the following steps: providing purified exosome, and mixing the purified exosome with hair growth active ingredients in a PBS (Phosphate Buffer Solution) with the pH value of 7.0-7.6, adding hydroxypropyl-beta-cyclodextrin with the final concentration of 0.5%-2% (w / v) and trehalose with the final concentration of 5%-10% (w / v), mildly incubating for 1-2 hours under the conditions that the temperature is 35-37 DEG C and the speed is 50-100 rpm, purifying, and freeze-drying to obtain a solid product. According to the invention, through the synergistic effect of the specific membrane permeability enhancer and the membrane stabilization protective agent, the efficient loading of the hair growth active component and the natural biological activity of the exosome are considered, the process is mild and controllable, the industrial amplification is easy, and the solid preparation solves the problem of inconvenient storage and transportation, and has strong practicability.

There is provided a method for producing a product by cells, the method including filtering a cell broth through a porous membrane to obtain a product by the cells, in which the porous membrane has a liquid contact surface having at least any one selected from the group consisting of an opening ratio of 57.5% or less, a pore diameter having a coefficient of variation of 0.5 or less, and a ratio of pore diameter / fiber diameter of 1.3 or less, and the liquid contact surface has at least any one selected from the group consisting of a structural anisotropy of 0.97 or more and 1.03 or less and a ratio of long pore diameter / short pore diameter of 1.8 or less, and a shear stress due to a flow of the cell broth on the liquid contact surface is set to 4.0 N / m2 or less.

The invention particularly relates to a virus-removing portable filtering device, which comprises: a shell, one end of the shell is provided with a water outlet part, and the interior of the shell is provided with a hollow part; the hollow fiber membrane is contained in the shell, raw water is input into the shell from the outside of the shell and output from the water outlet part after being filtered by the hollow fiber membrane, and antiviral material particles are doped in the hollow fiber membrane. Through the integrated embedding design of the antiviral material particles and the base material, the problem of combination stability existing in a surface coating process is solved, pore channels of the membrane can be completely reserved, and pore blockage caused by introduction of functional components is avoided; the water permeability of the membrane is guaranteed while the efficient virus interception performance is guaranteed, the problems that the water flux is reduced and the operation pressure is increased due to pore blockage are avoided, and the filtering efficiency and the operation stability can be both considered.

The application discloses a novel preparation for inhibiting botrytis cinerea and application thereof, and relates to the technical field of bacteriostatic drugs, and has the technical scheme as follows: the active ingredient of the preparation is composed of isoeugenol and bacillus velezensis fermentation liquor.The half bacteriostatic concentration EC50 value of the novel preparation reaches 59.7 ug / mL, and the bacteriostatic capacity is higher than that of the existing technology of bacto in the same concentration.Based on the influence on the membrane permeability of filamentous fungi, the application can synergistically inhibit the pathogenic activity of postharvest fungi of fruits, and is expected to be used for preparing a novel preparation for preventing and treating high-sugar fruits after harvesting.The application is simple to use, environment-friendly, can be directly smeared on the surface of fruits in the planting stage, can not be directly contacted with the fruits in the storage period, and can provide the bacteriostatic capacity for the storage period by using the fumigation mode.The application provides a new choice for the biological prevention of fruits, and has good economic benefits and social benefits.

The present invention, based on a non-bubble-based sonoporation technique, provides an ultrasound-assisted delivery platform for drug-loaded chitosan nanoparticles. This platform comprises: the administration of drug-loaded chitosan nanoparticles and the application of ultrasound stimulation. The drug-loaded chitosan nanoparticles are delivered to a target site, where ultrasound waves are directly applied to induce cellular membrane deformation. The ultrasound mechanical force temporarily alters membrane permeability, thereby facilitating the intracellular delivery of the drug-loaded chitosan nanoparticles.

The invention relates to the technical field of nicotine salts, in particular to a nicotine salt as well as a preparation method and application thereof, and preparation raw materials comprise acetyl organic acid, nicotine and deionized water. According to the invention, not only is the membrane permeability of the nicotine acetylsalicylate improved, but also the stability of the nicotine acetylsalicylate at normal temperature is improved, no respiratory tract stimulation effect exists, and the safety and comfort of medication are improved.

The invention discloses a high-throughput drug membrane permeability calculation and analysis method and system based on a coarseness model and a storage medium. The method comprises the following steps: constructing a coarseness simulation system containing a phospholipid bilayer and a solvent environment; all-atom structure information of to-be-detected drug molecules is obtained, and molecular fragment correspondence is automatically predicted through a machine learning model based on a random forest, so that a coarse-grained topological structure compatible with a Martini force field is generated; placing the drug coarseness model in a simulation system, and executing molecular dynamics simulation with umbrella-shaped sampling along the normal direction of the membrane; and based on the simulated trajectory, reconstructing an average force potential by using a weighted histogram analysis method, and calculating a membrane permeability coefficient according to a heterogeneous dissolution-diffusion model. According to the method, machine learning and physical simulation are combined, full automation of small molecule parameterization is achieved, the problems that a traditional method depends on artificial experience and is low in efficiency are solved, and high-throughput and high-precision virtual screening of a large-scale compound library can be achieved with low calculation cost.

The invention discloses a method for promoting recombinant corynebacterium glutamicum to secrete chondroitin oligosaccharide by changing cell membrane permeability, and belongs to the field of fermentation engineering. According to the invention, a high-copy double-plasmid system is used in corynebacterium glutamicum for coupling synthesis and cutting of chondroitin through a genetic engineering means, so that the yield of chondroitin oligosaccharide synthesized by corynebacterium glutamicum is increased. In order to promote the secretion of chondroitin oligosaccharide, the permeability of cell membranes is increased by quantitatively and regularly adding a surfactant tween-40 in the fermentation process. Through high-copy plasmid expression, culture medium optimization, surfactant treatment and other strategies, the yield of chondroitin oligosaccharide generated by fermentation of the constructed recombinant corynebacterium glutamicum is increased by 37.5%.

The invention belongs to the technical field of biological medicine, and particularly relates to an aromatic heterocyclic compound, a preparation method thereof and application of the aromatic heterocyclic compound in tumor resistance. According to the aromatic heterocyclic compound disclosed by the invention, the structural design is based on a structure-activity optimization concept of a bisaryl structure, and the core pyrrolo aromatic ring structure can realize pi-pi stacking and hydrogen bond coordination on a molecular level, so that stable molecular recognition is favorably formed with a protein kinase active pocket or a microtubule binding region; a nitrogen atom of the heterocyclic ring part is used as a hydrogen bond acceptor and forms stable hydrogen bond interaction with a target protein hinge region or key amino acid residues, so that the binding capacity of the compound and a kinase target is enhanced; substituent groups on the aromatic ring part can influence coplanarity and hydrophobic interaction of molecules by adjusting electron cloud density, so that compatibility with a target protein hydrophobic pocket is enhanced; the polarity distribution of the substituent group gives consideration to the solubility and membrane permeability of the compound at the same time, so that the accessibility and pharmacokinetic stability of a pharmacophore at a cellular level are ensured.

The invention relates to a method for providing a water-soluble adduct and an ibuprofen adduct, wherein the membrane permeability of the adduct is improved and the classification according to the Biopharmaceutics Classification System is influenced.

The application belongs to the technical field of microorganisms, and discloses application of shikimic acid in preparation of antibacterial preparations for inhibiting staphyloxin synthesis. It is found that shikimic acid can inhibit synthesis of staphyloxin in methicillin-resistant Staphylococcus aureus (MRSA), reduce tolerance of the bacteria to oxidative stress, induce accumulation of intracellular active oxygen, and damage cell membrane structure and function, including reducing cell membrane order, inducing membrane depolarization, increasing membrane permeability, and leading to ATP leakage and energy metabolism disorder. A new strategy and preparation direction are provided for clinical treatment of multi-drug resistant Staphylococcus aureus infection, and the application has important theoretical value and application prospect.

The invention provides a two-dimensional ultrasonic response type nanosheet for treating infectious pneumonia as well as a preparation method and application of the two-dimensional ultrasonic response type nanosheet. The two-dimensional ultrasonic response type nanosheet disclosed by the invention is a two-dimensional nanosheet formed by self-assembly of high-molecular polymers including PCL-b-PDMAEMA, PCL, PCL-Ce6 and PCL-b-PDMAEMA-I < + > under the guidance of crystal growth, can generate ROS (reactive oxygen species) under ultrasonic stimulation and has a universal targeting function of bacteria; the spherical nano-particles can cause intracellular substance outflow and bacterial death by destroying the completeness of bacterial membranes and enhancing the permeability of the membranes, have unique spatial adaptability in a complex alveolar mucus environment, can wrap broad-spectrum drug-resistant bacteria to the greatest extent due to the soft and controllable geometric structure, have significantly improved adhesion strength compared with other spherical nano-particles, and can be used for preparing a drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant nano-particle. The morphology-size synergistic effect can improve the biological membrane penetration efficiency of the nanosheet in lung fluid, block the regeneration of bacteria and the development of drug resistance, and provide a precise treatment strategy with penetrating power and long-term effectiveness for deep respiratory tract infection.

The invention discloses a method for inhibiting resistant gene conjugational transfer based on a Mo monatomic catalyst activated peroxydisulfate system. The method is characterized in that the Mo monatomic catalyst activated peroxydisulfate system inhibits resistance gene conjugational transfer by activating oxidative stress response, destroying membrane permeability, interfering energy metabolism and inhibiting biological membrane formation, the Mo monatomic catalyst is a Mo / NC catalyst, and the catalyst can efficiently activate PDS to generate active species 1O2 and SO4. The method realizes effective removal of ARB and ARGs in water, and active species induce oxidative stress to trigger SOS reaction in the reaction process, so as to inhibit ATP synthesis and reduce energy supply, thereby inhibiting conjugation transfer of ARGs. Scientific basis and technical solution can be provided for promoting treatment of drug-resistant pollution in the water environment.

To provide an amino acid capable of improving the membrane permeability of peptide compounds, as well as a peptide compound including the amino acid.SOLUTION: The present invention provides an amino acid having a side chain capable of forming at least one intramolecular hydrogen bond.SELECTED DRAWING: None

The invention belongs to the technical field of permeability testing, and particularly relates to a nanofiltration separation membrane permeability testing device which comprises a bottom frame and a plurality of liquid inlet pipes, the upper side of the bottom frame is fixedly connected with a lower support, the upper side of the lower support is fixedly connected with a plurality of lower cylindrical shell units, and the upper side of the bottom frame is fixedly connected with an upper support. A plurality of lifting mechanisms are fixedly connected to the upper support, upper cylindrical shell units matched with the lower cylindrical shell units are fixedly connected to the multiple lifting mechanisms, and the lower cylindrical shell units and the upper cylindrical shell units are half shells. The lower cylindrical shell unit and the upper cylindrical shell unit are spliced to form a cylindrical shell unit, and the upper side of the bottom frame is further fixedly connected with a plurality of independent lifting supports arranged side by side. According to the invention, the permeability of the nano-filtration separation membrane in an arc state under different conditions can be simply tested.

The membrane permeability dynamic multi-step prediction method with unbalanced samples belongs to the field of sewage treatment.The present application aims to early warn future membrane fouling events.Firstly, in view of the overlapping and abrupt change of the data of the membrane bioreactor sewage treatment process, a data adaptive classification method is proposed to reconstruct the variable data set related to membrane fouling, so as to realize the continuous classification of the unbalanced samples of the data of the membrane bioreactor sewage treatment process; secondly, based on the correlation between the data individuals under time series, a dynamic multi-step prediction model of recursive fuzzy neural network based on attraction is proposed, and the cumulative error of the prediction model is reduced by adjusting the recursive term parameters; thirdly, a speed gradient descent algorithm with adaptive learning rate is designed to update the neural network parameters, so as to speed up the learning process and ensure the stability of the output. The results show that the method can ensure the high-precision prediction of the membrane permeability in the future period of time.

The invention discloses a glove membrane permeability testing method based on an ultraviolet spectrophotometer, and relates to the technical field of glove production, and the glove membrane permeability testing method comprises the following steps: step 1, fixing a glove membrane sample in a sealed holder; step 2, applying an osmotic solution containing an ultraviolet absorption substance to the outer side of the glove membrane, wherein the inner side of the glove membrane is provided with a receiving solution; thirdly, the pressure difference between the outer side of the glove film and the inner side of the glove film is manufactured through a constant-pressure device or a gravity driving system; 4, circularly sending the receiving liquid to an ultraviolet spectrophotometer, and monitoring the absorbance change of the receiving liquid in real time by using the ultraviolet spectrophotometer; and 5, calculating the permeation flux or the retention rate according to the relationship between the absorbance and the concentration of the receiving liquid. Therefore, according to the scheme, the detection precision is extremely high, the monomolecular layer permeation can be detected, the permeation solution and wavelength are replaced, the method is suitable for different chemical substances, environment friendliness is achieved, radioactive labeling or destructive operation is not needed, and the environment-friendly detection requirement of the glove film is met.

The application provides a kind of amphiphilic block copolymer nanomicelles loaded with PDRN and its preparation method and application, belong to the medical cosmetic technical field.The preparation method of the amphiphilic block copolymer nanomicelles loaded with PDRN includes the following steps: S1 provides the buffer solution containing amphiphilic block copolymer nanomicelles and PDRN simultaneously;S2 the buffer solution is sheared at 0~25 ℃ with 8000~15000 rpm, then mixed with 100~500 rpm, the preparation method can effectively improve the problem that the combination rate of amphiphilic block copolymer and PDRN is low, at the same time, the amphiphilic block copolymer nanomicelles loaded with PDRN prepared by the method also has the advantages of drug sustained release function and strong membrane permeability, so that PDRN can continuously and efficiently play a role.

This invention relates to a method for the simultaneous removal of antibiotic-resistant bacteria and resistance genes using a solar-driven interfacial heat-Fenton oxidation-like process. The method involves placing a photothermal film loaded with carbon black composite photothermal material in a photothermal sterilization reactor, introducing antibiotic-resistant wastewater, adding H2O2, turning on a xenon lamp to preheat the photothermal film, sealing the reactor, and allowing the wastewater to pass through the photothermal film under gravity. This invention utilizes a photothermal conversion material to absorb light radiation and convert it into thermal radiation, treating antibiotic-resistant bacteria through the high-temperature interfacial heat effect generated on the photothermal film. High-temperature interfacial heat causes localized heating of the bacterial cell membrane structure, leading to a certain degree of breakage of fatty acid chains in the bacterial membrane lipid bilayer. This increases cell membrane permeability, and the resulting physical damage damages the bacterial outer membrane structure, further increasing membrane permeability and thus disrupting membrane integrity.

This invention discloses a loose nanofiltration membrane for treating dye / salt mixed wastewater, its preparation method, and its application, belonging to the field of water treatment membrane technology. This invention uses diphosgene or triphosgene as a linear crosslinking agent to prepare a high-performance loose nanofiltration membrane by crosslinking some inexpensive biopolymers with three-dimensional dendritic structures. This linear crosslinking method and the three-dimensional dendritic structure of the polymer help reduce the density of the nanofiltration membrane and improve the permeability of inorganic salts, thereby achieving efficient separation of dyes and salts. The reduction in density also contributes to the improvement of nanofiltration membrane permeability. Furthermore, these biomolecules are rich in hydroxyl and amino groups, which endow the membrane material with good hydrophilicity, which is beneficial to improving the water permeability of the nanofiltration membrane. The preparation process of this invention is simple, the raw materials are readily available and low in cost, and the resulting loose nanofiltration membrane has a good effect on the separation of dye / salt mixed wastewater. This preparation method has promising prospects for large-scale application.

The present application relates to a method for detecting the degree of irreversible electroporation by scanning electrochemical microscopy (SECM), which realizes rapid and efficient detection of the degree of cell electroporation by measuring the membrane permeability (Pm) of cells after stimulation by a pulsed electric field. The method first measures the approach curve of the SECM probe to the cells, then converts the approach curve into the membrane permeability of the cells to complete the measurement of the degree of electroporation, and measures the survival rate curve of the cells by the existing detection method, establishes the relationship between the Pm value and the cell survival rate, verifies the detection of irreversible electroporation by the membrane permeability of the cells, and finds that the law of the change of the membrane permeability with the pulsed voltage is almost the same as the law of the change of the cell survival rate with the pulsed voltage, so the method for measuring the degree of cell electroporation by SECM is effective and reliable.

The invention discloses a gastrointestinal tract bioadhesive drug delivery system, and particularly relates to a dihydromyricetin clathrate-bioadhesive microsphere, a raw material dihydromyricetin clathrate, an optimized process of the dihydromyricetin clathrate, and application of the dihydromyricetin clathrate in ionizing radiation injury protection of nuclear radiation, nuclear medicine, tumor radiotherapy and the like. Compared with a traditional oral administration mode, the dihydromyricetin-bioadhesive administration prolongs the residence time of the medicine in gastrointestinal tracts and improves the membrane permeability of the medicine, the bioavailability of the medicine is remarkably improved, and the dihydromyricetin-bioadhesive administration can be used for preparing the protective medicine for preventing / treating ionizing radiation injury and has a wide application prospect. The invention particularly relates to application in preparation of drugs for preventing / treating radioactive intestinal injury and the like.

This invention discloses a drug-loaded microcapsule-type controlled-release formulation of trigonelline and its preparation method. The method includes: using microcrystalline cellulose pellets as an inert carrier, sequentially loading a trigonelline drug-loaded layer, a hydroxypropyl methylcellulose isolation layer, and a composite controlled-release membrane layer composed of ethyl cellulose, RS / RL acrylic resin, triethyl citrate, and talc using bottom-spray fluidized bed coating technology; and then filling the mixture into capsules after curing. This invention effectively isolates the drug from the controlled-release membrane layer through the isolation layer and utilizes the RS / RL resin compound to synergistically regulate membrane permeability, achieving stable controlled release of trigonelline. The resulting formulation shows a release rate of no more than 25% in 0.1 mol / L hydrochloric acid after 2 hours, and release rates of 20%-45%, 45%-75%, and 70%-95% after 4 hours, 8 hours, and 12 hours in pH 6.8 buffer solution, respectively. The release behavior is stable and controllable, effectively solving the problem of burst release of highly water-soluble drugs and prolonging the duration of action.

The application belongs to the technical field of nanofiltration membranes, and discloses a eutectic solvent-induced furan bio-based composite polyamide nanofiltration membrane, and a preparation method thereof, which comprises four steps of synthesis of difuran dimethylamine monomers, pretreatment of an ultrafiltration membrane, preparation of an aqueous phase / organic phase solution, and interfacial polymerization to form a membrane; the interfacial polymerization aqueous phase is a mixed solvent of a eutectic solvent-water, the difuran dimethylamine is a monomer, and the organic phase is a polyacyl chloride solution.The difuran dimethylamine with a biomass source is used as the monomer, and dependence on petroleum-based raw materials is eliminated; the eutectic solvent is used to solve the bottleneck of poor water solubility of the furan monomer, the monomer diffusion kinetics is controlled through high viscosity of the eutectic solvent, excessive cross-linking is inhibited, a uniform and dense polyamide separation layer is formed, the trade-off effect between traditional membrane permeability and selectivity is broken, and the synergistic improvement of high water flux and ultra-high Mg 2+ / Li + separation selectivity is realized, the prepared nanofiltration membrane has excellent permeability and magnesium-lithium separation efficiency, and can be widely applied in the fields of water treatment and lithium extraction from salt lakes.

The invention discloses an intelligent hydrogel dressing and a preparation method thereof, and belongs to the field of biomedical materials and intelligent controlled release systems. The dressing comprises a liposome microcapsule system and a piezoelectric ionic gel matrix, and gel is composed of dimethylaminoethyl methacrylate, hydroxyethyl methylacrylate, sulfonated sodium alginate and the like and can generate an electric signal under mechanical stimulation; the liposome microcapsule system entraps a drug for wound treatment, when an electric signal output by the piezoelectric gel acts on the liposome, the membrane permeability of the liposome can be triggered to be increased, and on-demand controlled release is realized. Furthermore, a three-stage embedding structure is constructed through arginine, so that the stability of the liposome in the gel can be remarkably improved. The dressing disclosed by the invention can realize the intelligent controlled release functions of self power supply, dynamic healing promotion, antibiosis and motion coupling, and is suitable for treating chronic wound difficult to heal.

There is provided a method for producing a product by cells, the method including filtering a cell broth through a porous membrane to obtain a product by the cells, in which the porous membrane has a liquid contact surface having at least any one selected from the group consisting of an opening ratio of 57.5% or less, a pore diameter having a coefficient of variation of 0.5 or less, and a ratio of pore diameter / fiber diameter of 1.3 or less, and the liquid contact surface has at least any one selected from the group consisting of a structural anisotropy of 0.97 or more and 1.03 or less and a ratio of long pore diameter / short pore diameter of 1.8 or less, and a shear stress due to a flow of the cell broth on the liquid contact surface is set to 4.0 N / m2 or less.

The invention belongs to the technical field of biological medicines, and particularly relates to an anti-tumor polypeptide and application thereof in tumor resistance. The anti-tumor polypeptide provided by the invention comprises a first polypeptide and / or a second polypeptide, the amino acid sequence of the first polypeptide is as shown in SEQ ID NO: 1; the amino acid sequence of the second polypeptide is as shown in SEQ ID NO: 2. The anti-tumor polypeptide is stable in chemical property, can selectively act on tumor cells through electrostatic interaction, then is inserted into a cell membrane phospholipid skeleton by utilizing a hydrophobic fragment in the structure of the anti-tumor polypeptide to destroy the integrity of a cell membrane, change membrane permeability, cause change of osmotic pressure of the tumor cells and finally cause cracking of the tumor cells, so that the anti-tumor effect is achieved. Good broad-spectrum anti-tumor activity is realized; the compound has small influence on normal cells, has good selectivity, and is suitable for being used as an active component of a clinical antitumor drug.

The utility model relates to the field of cyclic peptide membrane observation discloses a kind of cyclic peptide membrane permeability observation device, including device base, the inner wall of device base is slidably connected with moving block, the inner wall of moving block is provided with limit mechanism, the inner wall of device base is fixedly connected with square, the top inner wall of device base is elastically connected with sliding block by tension spring, the bottom of sliding block is provided with wheel, sliding block is provided with multiple groups, multiple groups sliding block are connected by cross bar, the top of device base is provided with detection device, the top of device base is provided with container, the limit mechanism includes L-shaped plate.In the utility model, by setting moving block, L-shaped plate and sliding block, wheel and other components, wheel can be quickly exposed device and contact with ground, it is convenient for subsequent whole device to adjust its position on plane, change the defect that part cyclic peptide membrane permeability observation device cannot be safely moved.

The invention belongs to the field of biological medicines, and particularly discloses an FN-coated drug-loaded chitosan nanogel system as well as a preparation method and application thereof. According to the system, chitosan nanogel (CS NGs) is taken as a carrier, an antifungal drug clotrimazole (CLO) is loaded, and fibronectin (FN) is coated on the surface of the chitosan nanogel through electrostatic adsorption. The nanogel system has a pH-responsive drug release characteristic, and the release rate in an acidic environment (pH 5.5) is significantly higher than that in a neutral environment (pH 7.4). The preparation method comprises the following steps: preparing the CS NGs by a micro-emulsion method, loading the medicine and coating the FN. The system can significantly improve the water solubility and membrane permeability of clotrimazole, prolong the vagina retention time and promote the proliferation and migration of epithelial cells, and has anti-inflammatory and tissue repair functions. Experiments show that the system has a remarkable treatment effect on vulvovaginal candidiasis (VVC) and can be used for preparing drugs for treating VVC.