68 results about "Membrane permeability" patented technology

The invention discloses a baicalin and boric acid antibacterial peptide assembly and a preparation method and application thereof, belongs to the technical field of biological medicine, and provides a baicalin and boric acid antibacterial peptide assembly which comprises baicalin and boric acid antibacterial peptide. The amino acid sequence of the boric acid antibacterial peptide is one of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5 or SEQ ID NO. 6. According to the baicalin and boric acid antibacterial peptide assembly, phenylboronic acid molecules introduced to the C end of boric acid antibacterial peptide polypeptide can form boric acid ester bonds with the baicalin, the water solubility and membrane permeability of the baicalin can be improved, the baicalin can enter bacterial cells more easily to play a role, and the baicalin and the boric acid antibacterial peptide can form a multi-target synergistic antibacterial effect; therefore, the antibacterial effect is obviously improved.

The invention discloses a preparation method of a high-activity exosome hair growth promoter, which comprises the following steps: providing purified exosome, and mixing the purified exosome with hair growth active ingredients in a PBS (Phosphate Buffer Solution) with the pH value of 7.0-7.6, adding hydroxypropyl-beta-cyclodextrin with the final concentration of 0.5%-2% (w / v) and trehalose with the final concentration of 5%-10% (w / v), mildly incubating for 1-2 hours under the conditions that the temperature is 35-37 DEG C and the speed is 50-100 rpm, purifying, and freeze-drying to obtain a solid product. According to the invention, through the synergistic effect of the specific membrane permeability enhancer and the membrane stabilization protective agent, the efficient loading of the hair growth active component and the natural biological activity of the exosome are considered, the process is mild and controllable, the industrial amplification is easy, and the solid preparation solves the problem of inconvenient storage and transportation, and has strong practicability.

The invention provides a self-polymerized microporous polymer hollow fiber ultrafiltration membrane as well as a preparation method and application thereof, and belongs to the technical field of ultrafiltration membranes. The preparation method comprises the following steps: mixing a self-polymerized microporous polymer (PIM), a plasticizer and an organic solvent to obtain a spinning solution, and carrying out wet spinning on the spinning solution in one step to obtain the hollow fiber ultrafiltration membrane. According to the invention, a self-polymerized microporous polymer (PIM) is added in the spinning process, and the PIM material realizes molecular-level self-assembly through dynamic covalent bonds (such as imine bonds and borate bonds) to form a pore channel continuous network structure, so that the performance of the ultrafiltration membrane is improved. The hollow fiber ultrafiltration membrane prepared from the self-polymerization microporous polymer with the specific structure has good hydrophilic performance, the permeability coefficient of the ultrafiltration membrane is 100 Lm <-2 > h <-1 > or above, efficient filtration can be achieved at low pressure, and energy consumption is reduced.

There is provided a method for producing a product by cells, the method including filtering a cell broth through a porous membrane to obtain a product by the cells, in which the porous membrane has a liquid contact surface having at least any one selected from the group consisting of an opening ratio of 57.5% or less, a pore diameter having a coefficient of variation of 0.5 or less, and a ratio of pore diameter / fiber diameter of 1.3 or less, and the liquid contact surface has at least any one selected from the group consisting of a structural anisotropy of 0.97 or more and 1.03 or less and a ratio of long pore diameter / short pore diameter of 1.8 or less, and a shear stress due to a flow of the cell broth on the liquid contact surface is set to 4.0 N / m2 or less.

The invention discloses a film-embedded conjugated oligomeric electrolyte based on a natural product, and the structure of the film-embedded conjugated oligomeric electrolyte takes a natural polyphenol compound as a conjugated skeleton part, so that the overall biocompatibility of molecules is improved, and the toxicity is reduced; alkyl chains with different lengths or numbers are used as side chain parts to adjust the compatibility with a cell membrane and promote the membrane embedding and retention of molecules, and the alkyl chains can disturb the phospholipid arrangement of the cell membrane and increase the membrane permeability after the molecules are embedded into the membrane; a positively charged group is used as an end group part, and a negatively charged cell membrane is anchored through electrostatic attraction, so that the local molecular concentration is improved. The cell membrane is modified by the embedded membrane conjugated oligomeric electrolyte derived from the natural product, so that the permeability of the cell membrane is improved, the cytotoxicity is reduced, and the catalytic efficiency of cells is improved. The cell membrane is modified by the embedded membrane conjugated oligomeric electrolyte derived from the natural product, so that the permeability of the cell membrane is improved, the cytotoxicity is reduced, and the catalytic efficiency of cells is improved.

The present invention discloses a method for promoting membrane fouling resistance of an anaerobic membrane bioreactor under the action of an electric field and its application, belonging to the technical field of in-situ control of membrane fouling. In the process of treating sewage, the present invention adopts a Ni / Fe LDH@C-BIO hybrid synthesized by coprecipitation and electrostatic attraction to prepare an anaerobic membrane bioreactor, and performs an electrochemical redox reaction under the action of an external electric field to achieve membrane fouling resistance. The Ni / Fe LDH@C-BIO hybrid used in the anti-membrane fouling method has a large amount of positive charge on its surface and can quickly hybridize with anaerobic sludge with a negative charge on its surface. The external electric field promotes the regulation of the electrical activity of the anaerobic sludge EPS, decomposes the main pollutants that cause membrane clogging, and alleviates the deposition of microbial flocs and their metabolites. At the same time, the LDH with acidified activated carbon as a conductive substrate has excellent specific surface area catalytic performance and conductive performance, accelerates electron transfer, thereby further improving the reaction rate and effectively improving the anti-fouling ability and membrane permeability of the membrane assembly of the anaerobic membrane bioreactor.

The invention discloses thiazolidinedione or a derivative thereof as well as a preparation method and application thereof, the structural formula of the thiazolidinedione or the derivative thereof is non-substituted or randomly substituted by at least one R, and R is halogen or hydroxyl. An activity detection experiment and a compound dissociation constant test experiment on the inhibition activity of aldose reductase and alpha-glucosidase on the compound prove that the compound disclosed by the invention is an efficient AKR1B1 / alpha-GC double-target inhibitor with excellent membrane permeability, and is capable of relieving hyperglycemia while preventing abnormal metabolism of polyhydric alcohols; by utilizing a double-target collaborative intervention strategy, the application prospect in preparation of related medicines for preventing and / or treating diabetic complications is good.

The invention particularly relates to a virus-removing portable filtering device, which comprises: a shell, one end of the shell is provided with a water outlet part, and the interior of the shell is provided with a hollow part; the hollow fiber membrane is contained in the shell, raw water is input into the shell from the outside of the shell and output from the water outlet part after being filtered by the hollow fiber membrane, and antiviral material particles are doped in the hollow fiber membrane. Through the integrated embedding design of the antiviral material particles and the base material, the problem of combination stability existing in a surface coating process is solved, pore channels of the membrane can be completely reserved, and pore blockage caused by introduction of functional components is avoided; the water permeability of the membrane is guaranteed while the efficient virus interception performance is guaranteed, the problems that the water flux is reduced and the operation pressure is increased due to pore blockage are avoided, and the filtering efficiency and the operation stability can be both considered.

The application discloses a novel preparation for inhibiting botrytis cinerea and application thereof, and relates to the technical field of bacteriostatic drugs, and has the technical scheme as follows: the active ingredient of the preparation is composed of isoeugenol and bacillus velezensis fermentation liquor.The half bacteriostatic concentration EC50 value of the novel preparation reaches 59.7 ug / mL, and the bacteriostatic capacity is higher than that of the existing technology of bacto in the same concentration.Based on the influence on the membrane permeability of filamentous fungi, the application can synergistically inhibit the pathogenic activity of postharvest fungi of fruits, and is expected to be used for preparing a novel preparation for preventing and treating high-sugar fruits after harvesting.The application is simple to use, environment-friendly, can be directly smeared on the surface of fruits in the planting stage, can not be directly contacted with the fruits in the storage period, and can provide the bacteriostatic capacity for the storage period by using the fumigation mode.The application provides a new choice for the biological prevention of fruits, and has good economic benefits and social benefits.

The present invention, based on a non-bubble-based sonoporation technique, provides an ultrasound-assisted delivery platform for drug-loaded chitosan nanoparticles. This platform comprises: the administration of drug-loaded chitosan nanoparticles and the application of ultrasound stimulation. The drug-loaded chitosan nanoparticles are delivered to a target site, where ultrasound waves are directly applied to induce cellular membrane deformation. The ultrasound mechanical force temporarily alters membrane permeability, thereby facilitating the intracellular delivery of the drug-loaded chitosan nanoparticles.

Macrocyclic peptides (MPs) are a class of compounds that have been shown to be particularly well suited for engaging difficult protein targets. However, their utility is limited by their generally poor cell permeability and bioavailability. The present disclosure reports an efficient solid-phase synthesis of novel MPs by trapping a reversible intramolecular imine linkage with a 2-carbonyl pyridine to create an imidazopyridinium (IP+)-linked ring. This chemistry is useful for the creation of macrocycles of different sizes and geometries, including head-to-side and side-to-side chain configurations. Many of the IP+-linked MPs exhibit far better passive membrane permeability than expected for "beyond Rule of 5" molecules, in some cases exceeding that of much lower molecular weight, traditional drug molecules. This chemistry has been demonstrated to be suitable for the creation of libraries of IP+-linked MPs and show that these libraries can be mined for protein ligands.

The invention relates to the technical field of nicotine salts, in particular to a nicotine salt as well as a preparation method and application thereof, and preparation raw materials comprise acetyl organic acid, nicotine and deionized water. According to the invention, not only is the membrane permeability of the nicotine acetylsalicylate improved, but also the stability of the nicotine acetylsalicylate at normal temperature is improved, no respiratory tract stimulation effect exists, and the safety and comfort of medication are improved.

The invention discloses a high-throughput drug membrane permeability calculation and analysis method and system based on a coarseness model and a storage medium. The method comprises the following steps: constructing a coarseness simulation system containing a phospholipid bilayer and a solvent environment; all-atom structure information of to-be-detected drug molecules is obtained, and molecular fragment correspondence is automatically predicted through a machine learning model based on a random forest, so that a coarse-grained topological structure compatible with a Martini force field is generated; placing the drug coarseness model in a simulation system, and executing molecular dynamics simulation with umbrella-shaped sampling along the normal direction of the membrane; and based on the simulated trajectory, reconstructing an average force potential by using a weighted histogram analysis method, and calculating a membrane permeability coefficient according to a heterogeneous dissolution-diffusion model. According to the method, machine learning and physical simulation are combined, full automation of small molecule parameterization is achieved, the problems that a traditional method depends on artificial experience and is low in efficiency are solved, and high-throughput and high-precision virtual screening of a large-scale compound library can be achieved with low calculation cost.

There is provided a method for producing a product by cells, the method including filtering a cell broth through a porous membrane to obtain a product by the cells, in which the porous membrane has a liquid contact surface having at least any one selected from the group consisting of an opening ratio of 57.5% or less, a pore diameter having a coefficient of variation of 0.5 or less, and a ratio of pore diameter / fiber diameter of 1.3 or less, and the liquid contact surface has at least any one selected from the group consisting of a structural anisotropy of 0.97 or more and 1.03 or less and a ratio of long pore diameter / short pore diameter of 1.8 or less, and a shear stress due to a flow of the cell broth on the liquid contact surface is set to 4.0 N / m2 or less.

The invention relates to the technical field of albumin oligopeptide enzymolysis, in particular to an albumin oligopeptide enzymolysis preparation process which comprises the following steps: (1) constructing and expressing recombinant albumin containing a KRPAGY repetitive sequence; (2) purifying the recombinant albumin; (3) carrying out enzymolysis on the purified recombinant albumin by using a multiple enzymolysis system; (4) purifying the enzymolysis product through a multi-stage membrane separation system; (5) carrying out structural modification on the purified peptide fragment; and (6) evaluating the immunocompetence of the modified peptide fragment, through the design of a multiple enzymolysis system, accurate hydrolysis of albumin is realized, and the yield and specificity of the target peptide fragment are remarkably improved. The innovative membrane separation technology greatly improves the separation and purification efficiency; through N-terminal cysteine modification and C-terminal glycyl-prolyl modification, the stability and membrane permeability of the peptide fragment in the gastrointestinal tract environment are significantly improved.

The present invention discloses a Nile blue derivative compound, its preparation method, and application. The Nile blue derivative compound provided has the following general structural formula (I). The Nile blue derivative compound exhibits good structural stability, excellent membrane permeability, and strong antioxidant properties. It can be used as a mitochondrial single-molecule localization imaging probe. It not only has good mitochondrial targeting, but also can specifically label mitochondria through simple co-incubation without the need for transfection, resulting in high labeling efficiency and no damage to cells or mitochondria. #imgabs0#

The invention discloses a method for promoting recombinant corynebacterium glutamicum to secrete chondroitin oligosaccharide by changing cell membrane permeability, and belongs to the field of fermentation engineering. According to the invention, a high-copy double-plasmid system is used in corynebacterium glutamicum for coupling synthesis and cutting of chondroitin through a genetic engineering means, so that the yield of chondroitin oligosaccharide synthesized by corynebacterium glutamicum is increased. In order to promote the secretion of chondroitin oligosaccharide, the permeability of cell membranes is increased by quantitatively and regularly adding a surfactant tween-40 in the fermentation process. Through high-copy plasmid expression, culture medium optimization, surfactant treatment and other strategies, the yield of chondroitin oligosaccharide generated by fermentation of the constructed recombinant corynebacterium glutamicum is increased by 37.5%.

The invention belongs to the technical field of biological medicine, and particularly relates to an aromatic heterocyclic compound, a preparation method thereof and application of the aromatic heterocyclic compound in tumor resistance. According to the aromatic heterocyclic compound disclosed by the invention, the structural design is based on a structure-activity optimization concept of a bisaryl structure, and the core pyrrolo aromatic ring structure can realize pi-pi stacking and hydrogen bond coordination on a molecular level, so that stable molecular recognition is favorably formed with a protein kinase active pocket or a microtubule binding region; a nitrogen atom of the heterocyclic ring part is used as a hydrogen bond acceptor and forms stable hydrogen bond interaction with a target protein hinge region or key amino acid residues, so that the binding capacity of the compound and a kinase target is enhanced; substituent groups on the aromatic ring part can influence coplanarity and hydrophobic interaction of molecules by adjusting electron cloud density, so that compatibility with a target protein hydrophobic pocket is enhanced; the polarity distribution of the substituent group gives consideration to the solubility and membrane permeability of the compound at the same time, so that the accessibility and pharmacokinetic stability of a pharmacophore at a cellular level are ensured.

The invention relates to a method for providing a water-soluble adduct and an ibuprofen adduct, wherein the membrane permeability of the adduct is improved and the classification according to the Biopharmaceutics Classification System is influenced.

The application belongs to the technical field of microorganisms, and discloses application of shikimic acid in preparation of antibacterial preparations for inhibiting staphyloxin synthesis. It is found that shikimic acid can inhibit synthesis of staphyloxin in methicillin-resistant Staphylococcus aureus (MRSA), reduce tolerance of the bacteria to oxidative stress, induce accumulation of intracellular active oxygen, and damage cell membrane structure and function, including reducing cell membrane order, inducing membrane depolarization, increasing membrane permeability, and leading to ATP leakage and energy metabolism disorder. A new strategy and preparation direction are provided for clinical treatment of multi-drug resistant Staphylococcus aureus infection, and the application has important theoretical value and application prospect.

The invention provides a two-dimensional ultrasonic response type nanosheet for treating infectious pneumonia as well as a preparation method and application of the two-dimensional ultrasonic response type nanosheet. The two-dimensional ultrasonic response type nanosheet disclosed by the invention is a two-dimensional nanosheet formed by self-assembly of high-molecular polymers including PCL-b-PDMAEMA, PCL, PCL-Ce6 and PCL-b-PDMAEMA-I < + > under the guidance of crystal growth, can generate ROS (reactive oxygen species) under ultrasonic stimulation and has a universal targeting function of bacteria; the spherical nano-particles can cause intracellular substance outflow and bacterial death by destroying the completeness of bacterial membranes and enhancing the permeability of the membranes, have unique spatial adaptability in a complex alveolar mucus environment, can wrap broad-spectrum drug-resistant bacteria to the greatest extent due to the soft and controllable geometric structure, have significantly improved adhesion strength compared with other spherical nano-particles, and can be used for preparing a drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant drug-resistant nano-particle. The morphology-size synergistic effect can improve the biological membrane penetration efficiency of the nanosheet in lung fluid, block the regeneration of bacteria and the development of drug resistance, and provide a precise treatment strategy with penetrating power and long-term effectiveness for deep respiratory tract infection.

The invention discloses a method for inhibiting resistant gene conjugational transfer based on a Mo monatomic catalyst activated peroxydisulfate system. The method is characterized in that the Mo monatomic catalyst activated peroxydisulfate system inhibits resistance gene conjugational transfer by activating oxidative stress response, destroying membrane permeability, interfering energy metabolism and inhibiting biological membrane formation, the Mo monatomic catalyst is a Mo / NC catalyst, and the catalyst can efficiently activate PDS to generate active species 1O2 and SO4. The method realizes effective removal of ARB and ARGs in water, and active species induce oxidative stress to trigger SOS reaction in the reaction process, so as to inhibit ATP synthesis and reduce energy supply, thereby inhibiting conjugation transfer of ARGs. Scientific basis and technical solution can be provided for promoting treatment of drug-resistant pollution in the water environment.

The invention relates to the technical field of gas type identification, in particular to a fire-fighting field gas identification method based on flux and bubble characteristics of a gas separation membrane, which comprises the following steps: step 1, data acquisition: preparing a mixed gas sample containing to-be-tested gas, and introducing the mixed gas sample into a test device provided with a selected gas separation membrane at different pressures to obtain a test sample; collecting pressure, concentration and flow velocity data of gas on two sides of the membrane by using a sensor; 2, preprocessing: correcting temperature and pressure conditions of the acquired data; according to the method, a gas permeability coefficient correction model and bubble dynamic characteristic analysis are introduced, a multi-parameter fused gas recognition mechanism is established, the flux parameters and bubble characteristics of gas in the membrane are combined, the limitation of single parameter detection is avoided, the recognition precision is greatly improved, and the recognition accuracy is improved. And meanwhile, the stability and adaptability of fire-fighting field detection are improved by combining the influence of environmental factors such as compensation temperature and pressure on the membrane permeability characteristic.

To provide an amino acid capable of improving the membrane permeability of peptide compounds, as well as a peptide compound including the amino acid.SOLUTION: The present invention provides an amino acid having a side chain capable of forming at least one intramolecular hydrogen bond.SELECTED DRAWING: None

The present invention provides an evaluation method for the degree of clogging and contamination of ultrafiltration membranes, which belongs to the field of membrane water treatment technology. The evaluation method uses the UF normalized membrane permeability reduction ratio (R i ) as an assessment indicator of membrane clogging and fouling, categorized into three levels of fouling, this method eliminates the influence of factors such as temperature and sludge concentration on membrane permeability changes, objectively and directly assessing membrane permeability and, consequently, determining the degree of membrane clogging and fouling. This provides a basis for developing and optimizing UF membrane cleaning and maintenance programs, facilitating the scientific and rational determination of membrane cleaning and maintenance cycles, improving membrane operating efficiency, and extending membrane service life. Furthermore, this method establishes a rapid increase in the percentage decrease in UF normalized membrane permeability as a characteristic indicator of severe membrane clogging and irreversible fouling, enabling an effective, accurate, and rapid assessment of the membrane's current condition.

The present invention discloses a 16-amino acid polypeptide AA16 extracted from the LPS-binding protein CD14. 13 derivative polypeptides are obtained by amino acid replacement to increase α-helicity, improve amphiphilicity, and change the number of positive charges and hydrophobicity, thereby obtaining a targeting peptide with specific resistance to Gram-negative bacteria. Among them, the derivative polypeptide AA16-10R is specifically resistant to Gram-negative bacteria, with minimum inhibitory concentrations of 32 μg / mL and 16 μg / mL for Pseudomonas aeruginosa and Escherichia coli, respectively. It can bind to LPS on the bacterial membrane in the form of an α-helix, increase membrane permeability, and ultimately cause bacterial death, thereby having a good antibacterial effect.

The invention relates to the technical field of fluorescent probes, and discloses a preparation method and application of a cytoplasmic membrane covalent labeling fluorescent probe based on non-membrane permeability, and the preparation method comprises the following steps: S1, synthesizing 4-(2, 3, 3-trimethyl-3H-indole-1-onium-1-yl) butane-1-sulfonate; s2, synthesizing a 2-halogen substituted N-((1E, 2Z)-3-9 (phenylamino) allylidene) phenylamine derivative; and S3, synthesizing a target product, namely the Sulfo-Cy5-X fluorescent probe. The novel cytoplasmic membrane fluorescent probe is prepared by adopting a membrane impermeability-based cytoplasmic membrane covalent labeling principle and introducing a sulfonic acid group with high water solubility and a covalent reaction site, so that a long-time and specific labeling function on the cytoplasmic membrane is realized; according to the method, the problems of short imaging time and low water solubility of a traditional fluorescent probe are solved, and a more ideal tool is provided for in-situ observation of dynamic change of the cytoplasmic membrane.

The present invention discloses the preparation of a peptide nanotube-cyclic diguanosine monophosphate complex for tumor treatment, which method improves the problems of poor membrane permeability and low bioavailability of cyclic diguanosine monophosphate (c-di-GMP). Peptide nanotubes (PNT) and phenylboronic acid peptide nanotubes (PPNT) are used to deliver c-di-GMP, which can enhance the immune anti-tumor effect of c-di-GMP in vivo.

The invention belongs to the technical field of permeability testing, and particularly relates to a nanofiltration separation membrane permeability testing device which comprises a bottom frame and a plurality of liquid inlet pipes, the upper side of the bottom frame is fixedly connected with a lower support, the upper side of the lower support is fixedly connected with a plurality of lower cylindrical shell units, and the upper side of the bottom frame is fixedly connected with an upper support. A plurality of lifting mechanisms are fixedly connected to the upper support, upper cylindrical shell units matched with the lower cylindrical shell units are fixedly connected to the multiple lifting mechanisms, and the lower cylindrical shell units and the upper cylindrical shell units are half shells. The lower cylindrical shell unit and the upper cylindrical shell unit are spliced to form a cylindrical shell unit, and the upper side of the bottom frame is further fixedly connected with a plurality of independent lifting supports arranged side by side. According to the invention, the permeability of the nano-filtration separation membrane in an arc state under different conditions can be simply tested.