27 results about "Isoeugenol" patented technology

The invention relates to a method for increasing the sweetness value of carbohydrates by using an aroma substance containing isoeugenol, which comprises the following steps: mixing the carbohydrates and the aroma substance containing isoeugenol, and exploring the influence on the sweetness response value by using an electronic tongue and a molecular simulation technology. The aroma substance containing isoeugenol comprises isoeugenol or an isoeugenol capsule clathrate compound, the carbohydrate substance comprises cane sugar, and the isoeugenol capsule clathrate compound is an isoeugenol / gamma-cyclodextrin capsule clathrate compound. Compared with the prior art, the sweetening effect of the isoeugenol is obvious under the cane sugar concentration of 7wt%-9wt%; the sweetening effect of the isoeugenol capsule clathrate compound is higher than that of isoeugenol under the same condition, the aroma is more lasting and soft at high temperature, the sweet response value is larger, molecular docking shows that the capsule clathrate compound further strengthens the interaction between sucrose and sweet receptor protein, a theoretical support is provided for precise regulation and control of food flavor, and the isoeugenol capsule clathrate compound has good application prospects. The method can be used for developing novel food and beverage products.

The invention discloses an application of an isoeugenol monooxygenase mutant in production of isoeugenol vanillin, which comprises the steps of recombinant strain construction, fermentation culture of recombinant strains and catalytic reaction of fermentation broth, and the isoeugenol monooxygenase mutant is mutated through an isoeugenol monooxygenase amino acid sequence of wild fungi WT. And the thalli are expressed by escherichia coli, so that a series of thalli with a promoting effect on the catalytic efficiency of vanillin production are formed. The mutant super-efficient enzyme catalyst with the catalytic conversion rate reaching up to 98.5% or above is obtained through screening, the defects of low efficiency, pollution, high energy consumption and the like of traditional chemical catalyst production are overcome, and the mutant super-efficient enzyme catalyst is more suitable for green industrial production requirements and worthy of being popularized to industrial use.

The invention relates to the field of coatings, in particular to a solvent-free epoxy coating for a drinking water tank and a preparation method thereof.The solvent-free epoxy coating is prepared from a component A and a component B. The component A comprises 40-60 parts of epoxy resin, 5-20 parts of reactive diluent, 0.3-0.5 part of dispersing agent, 0.1-0.8 part of defoaming agent, 0.6-1.2 parts of thixotropic agent, 20-35 parts of pigment and 10-20 parts of filler; the epoxy resin comprises bisphenol A epoxy vinyl ester resin and hydrogenated bisphenol A resin; the active diluent comprises a cardanol-based active diluent and 4-epoxy isoeugenol; the dispersing agent comprises a polymeric dispersing agent and polyurethane acrylate porous microspheres; the component B comprises a modified alicyclic amine curing agent and cardanol carbamate, and the mass ratio of the modified alicyclic amine curing agent to the cardanol carbamate is (1.8-5): 1; the amine value of the modified alicyclic amine curing agent is 360 to 420 mgKOH / g. The solvent-free epoxy coating for the drinking water tank is non-toxic, has relatively strong adhesive force, water resistance, flexibility and antibacterial property and relatively long service life, and is suitable for various drinking water tanks.

The invention discloses a 4-propyl guaiacol oxidase mutant as well as a preparation method and application thereof, wild type 4-propyl guaiacol oxidase comes from Penicillium simplicissimum, an active center of the wild type 4-propyl guaiacol oxidase is modified by using a directed evolution means, and the mutant is obtained by modifying the active center of the wild type 4-propyl guaiacol oxidase. 4-propyl guaiacol oxidase mutants D170E, S426T, S50Q / D170E, D170E / L411V and D170E / L411V / S106C with relatively high activity, cis-trans selectivity and trans-isoeugenol yield are obtained, efficient synthesis from cheap 4-propyl guaiacol to high-value trans-isoeugenol is realized, and industrial synthesis and application of the trans-isoeugenol are facilitated.

The invention relates to the technical field of circuit board processing, in particular to an efficient environment-friendly acidic degreaser and a preparation method thereof. An efficient environment-friendly acidic degreasing agent is prepared from the following preparation raw materials in parts by mass: 30-40 parts of inorganic acid, 12-15 parts of a corrosion inhibitor, 40-50 parts of a wetting agent, 10-12 parts of an emulsion stabilizer, 8-10 parts of isoeugenol, 15-18 parts of plant degreasing liquid and 850-870 parts of deionized water. The efficient and environment-friendly acidic degreaser has a rapid and excellent degreasing effect, can effectively protect metal on the surface of a circuit board and improve the binding force of a plating layer, has the characteristics of good high and low temperature stability, environmental protection, simple preparation method, low production cost, excellent comprehensive performance and the like, and is suitable for electroplating acidic degreasing procedures of various circuit boards.

The application discloses 4-propyl guaiacol oxidase mutants, a preparation method and application thereof, wherein wild-type 4-propyl guaiacol oxidase is from Penicillium simplicissimum Penicillium simplicissimum ​ The active center of the wild-type 4-propyl guaiacol oxidase is reformed by using a directed evolution method, and 4-propyl guaiacol oxidase mutants D170E, S426T, S50Q / D170E, D170E / L411V and D170E / L411V / S106C with high activity, cis-trans selectivity and trans-isoeugenol yield are obtained, efficient synthesis of high-value trans-isoeugenol from cheap 4-propyl guaiacol is realized, and the industrialized synthesis and application of the trans-isoeugenol are facilitated.

The application discloses a novel preparation for inhibiting botrytis cinerea and application thereof, and relates to the technical field of bacteriostatic drugs, and has the technical scheme as follows: the active ingredient of the preparation is composed of isoeugenol and bacillus velezensis fermentation liquor.The half bacteriostatic concentration EC50 value of the novel preparation reaches 59.7 ug / mL, and the bacteriostatic capacity is higher than that of the existing technology of bacto in the same concentration.Based on the influence on the membrane permeability of filamentous fungi, the application can synergistically inhibit the pathogenic activity of postharvest fungi of fruits, and is expected to be used for preparing a novel preparation for preventing and treating high-sugar fruits after harvesting.The application is simple to use, environment-friendly, can be directly smeared on the surface of fruits in the planting stage, can not be directly contacted with the fruits in the storage period, and can provide the bacteriostatic capacity for the storage period by using the fumigation mode.The application provides a new choice for the biological prevention of fruits, and has good economic benefits and social benefits.

The present invention relates to the technical field of metal surface treatment, specifically a high-strength steel pipe based on hot-dip galvanizing and a preparation process thereof. The present invention first performs hot-dip galvanizing treatment on the steel pipe, and then sprays a passivating agent for treatment; the passivating agent is compounded by a composite coating, an amine curing agent, and water; in the composite coating, an epoxy-modified acrylic resin is polymerized by methyl methacrylate, 4-epoxyisoeugenol, and butyl acrylate, and a silicone oil-modified epoxy resin is first reacted with hydroxy silicone oil and isophorone diisocyanate to generate terminal isocyanate silicone oil, and then reacted with an isocyanate group and a hydroxyl group in an epoxy resin to obtain; under the action of an amine curing agent, cross-linking occurs between the epoxy-modified acrylic resin and the silicone oil-modified epoxy resin, thereby forming a dense protective layer. The process of the present invention can effectively protect the hot-dip galvanized steel pipe from corrosion and improve the service life of the steel pipe.

The application discloses a method for preparing vanillin by using ferulic acid as raw material, and belongs to the technical field of organic chemical synthesis, which uses ferulic acid as raw material, reduces the reaction of isomerization of eugenol into isoeugenol, avoids using a large amount of alkali solution, and has the characteristics of clean and environmental protection, simple process, less side reaction, high product yield, etc.; the method comprises the following steps: (1) taking ferulic acid, adding an alcohol solvent, ferric chloride and hydrogen peroxide, and reacting after heating; (2) concentrating and recovering the alcohol solvent from the reaction liquid obtained in the step (1); (3) adding an ester solvent and an alkali aqueous solution to the alcohol solvent obtained in the step (2) for washing, layering after standing, and collecting the ester solvent layer solution; (4) concentrating and recovering the ester solvent from the ester solvent layer solution obtained in the step (3), recrystallizing the obtained solid with an ethanol aqueous solution, performing suction filtration, washing with water, and drying to obtain a finished product.

The invention provides a method for preparing vanillin from eugenol, and belongs to the technical field of organic synthesis, and the method comprises the following steps: S1, preparing a Ni-Mo / aluminosilicate / carbon-based material; s2, preparing isoeugenol; s3, isoeugenol, the Ni-Mo / aluminosilicate / carbon-based material, absolute ethyl alcohol and deionized water are added into a closed pressure-resistant reaction kettle to be mixed, a sodium hydroxide solution is dropwise added to be stirred, the temperature is increased, oxygen is introduced, stirring reaction, cooling, separation, pH adjustment to be acidic, aging, suction filtration and washing are conducted, the mixture is added into an ethanol water solution to be heated and stirred, decoloration, filtration, recrystallization and washing are conducted, and the Ni-Mo / aluminosilicate / carbon-based material is obtained. And drying in vacuum to obtain vanillin. According to the invention, side reactions can be effectively inhibited, and the yield and purity of the target product are improved.

The invention provides an essential oil preparation method capable of regulating and controlling components of clove bud oil on line. The invention also provides the clove bud oil prepared by the method and application thereof. The invention further provides the cigarette aroma raw material containing the clove bud oil and the cigarette. The essential oil preparation method and the application have the advantages of raw material advantages, mature technology, adjustable fragrance, simple operation, catalyst advantages, cost saving and wide application range, can obtain the required clove bud oil with an adjustable eugenol and isoeugenol ratio at a high yield, and can be used for preparing the essential oil with the adjustable eugenol and isoeugenol ratio. Therefore, the requirements of cigarette flavor blending application are met, and the practical application value is achieved.

The invention provides a blood-sucking midge efficient repellent and a screening and identifying method thereof, and relates to the technical field of biology. According to the invention, an odor receptor gene FtaiOr1 which is highly expressed at the head of a female gnat is obtained through identification, and a transgenic drosophila melanogaster strain Or22ab- / -GAL4 which can be used for screening a blood-sucking midge repellent compound is constructed through heterologous expression of the gene in a drosophila melanogaster ab3A neuron; and UAS-FtaiOr1 (UAS-FtaiOr1). Six blood sucking midge repelling compounds (camphor, eudesmol, (+)-anisone, alpha-terpilenol, (S)-cis-verbenol and isoterpinene) are obtained by screening of the fruit fly strain. The compounds can be used independently or in a compounded manner, are friendly to non-target organisms and environment, can be directly applied to repelling and on-site prevention and control of blood sucking midges, and have important application value in the aspects of reducing midge bites and reducing the propagation risk of entomophilous diseases.

The invention provides a preparation method of 3-methoxy-4-hydroxybenzaldehyde, and belongs to the technical field of organic synthesis. The preparation method comprises the following steps: taking isoeugenol as an initial raw material, carrying out electron transfer with a photocatalyst under the irradiation of visible light, taking oxygen as an oxidizing agent to form epoxide, oxidizing propenyl into aldehyde group under the irradiation of visible light, and finally breaking the epoxide to form vanillin. The synthesis method disclosed by the invention is simple in steps, the initial raw material is isoeugenol which is cheap and easy to obtain, air can be used as an oxidant, strong oxidants such as ozone are not needed, the use of high-pollution and high-toxicity reagents is reduced, and the method is safe to operate and environment-friendly; the reaction conditions are mild, strict low-temperature conditions are not needed, the reaction time is short, and the method is suitable for industrial production.

The invention relates to an isoeugenol monooxygenase mutant and an application of the isoeugenol monooxygenase mutant in preparation of vanillin. In particular to an isoeugenol monooxygenase mutant, nucleic acid for coding the isoeugenol monooxygenase mutant, a recombinant expression vector containing the nucleic acid, a recombinant expression transformant containing the recombinant expression vector, an isoeugenol monooxygenase mutant catalyst and preparation of the isoeugenol monooxygenase mutant catalyst. The invention also discloses an application of the isoeugenol monooxygenase mutant or the isoeugenol monooxygenase mutant catalyst in preparation of vanillin by taking isoeugenol as a substrate. The specific activity of the mutant I59L / L470I is 1.32 times that of a parent, and the selectivity of the mutant I59L / L470I is 1.40 times that of the parent. The mutant enzyme can catalyze propenyl on a benzene ring of a substrate isoeugenol so as to generate vanillin, the isoeugenol can be used as the substrate, and the isoeugenol can be completely converted to synthesize vanillin under the conditions that the reaction temperature is 25 DEG C, the pH value is 10.0, the cell quantity is 20g / L and the conversion time is 8 hours. And the conversion rate can reach 100% under the condition that the substrate concentration is 100mM, so that the method has a wide application prospect.

The application provides a continuous flow synthesis method of isopinocamphe 4,8-epoxide ‑ The reaction system has the advantages of less OH, weaker alkalinity, moderate oxidizability, mild reaction condition, directional oxidation of the double bond at the 4th position, high oxidation selectivity, and the selectivity of the target product, isopinocamphe 4,8-epoxide, can reach more than 90%. Meanwhile, the application overcomes the defect that the presence of water in the existing catalytic system can easily lead to ring-opening hydrolysis of the epoxidation reaction, greatly accelerates the reaction process of the epoxidation reaction, increases the yield of the product, and the yield can reach more than 84%. The reaction system has the advantages of mild reaction system condition, high oxidation selectivity, simple product separation, less side reaction, and easy treatment and recovery of waste liquid. The raw material conversion rate of the method is high, the selectivity and yield of the target product, isopinocamphe 4,8-epoxide, are high, the method is easy to realize industrialization, and is friendly to the environment.

The invention relates to vanillin series electronic atomization liquid, a preparation method and an electronic atomization device. The vanillin series electronic atomized liquid is prepared from the following components in parts by weight: 0 to 99.9979 parts of a solvent, 0.002 to 15 parts of sweetening agent molecules and 0.0001 to 20 parts of vanillin series nerve sweet sensing molecules. The vanillin series nerve sweet sensing molecules comprise ethyl vanillin, vanillin, isoeugenol, junifolene and eugenol. The invention further discloses a preparation method of the vanillin series nerve sweet sensing molecules. The vanillin series electronic atomized liquid provided by the invention not only contains conventional sweetening agent molecules, but also is added with vanillin series nerve sweet sense molecules, and under the combined action of the sweetening agent molecules and the vanillin series nerve sweet sense molecules, the electronic atomized liquid can present high sweetness after being atomized, and is comfortable in mouth feel and suitable for smoking.

The application discloses application of isoeugenol monooxygenase mutants in isoeugenol vanillin production, and comprises recombinant strain construction, fermentation culture of the recombinant strain and catalytic reaction of a fermentation liquor, wherein the isoeugenol monooxygenase mutants are obtained by mutating isoeugenol monooxygenase amino acid sequences of wild-type bacteria WT and expressing the obtained bacterial bodies by using Escherichia coli, and a series of bacterial bodies which promote catalytic efficiency of vanillin production are formed. The application screens mutant super-high-efficiency enzyme catalysts with a catalytic conversion rate of 98.5% or above, avoids the shortcomings of traditional chemical catalyst production, such as low efficiency, pollution and high energy consumption, and makes the application more suitable for green industrial production requirements, so that the application is worthy of popularization and industrial use.

The invention discloses a vanillyl alcohol oxidase mutant as well as a preparation method and application thereof, wild type vanillyl alcohol oxidase comes from Penicillium simplicissimum, an active center of the wild type vanillyl alcohol oxidase is modified by using a directed evolution means, and the vanillyl alcohol oxidase mutant with relatively high activity, cis-trans selectivity and cis-isoeugenol yield is obtained. The mutation is at least one of S106 L, L316F or I468 L. According to the invention, efficient synthesis from cheap 4-propyl guaiacol to cis-isoeugenol is realized, and industrial synthesis and application of cis-isoeugenol are facilitated.

The invention belongs to the technical field of dermatobiology and functional cosmetics / skin medicines, and particularly relates to a composition for synergistically inhibiting melanogenesis through isorosmarinol and isoasiaticoside and application of the composition. The composition in the present application contains isorosmarinol and isoasiaticoside as active ingredients. Experiments prove that the composition can reduce the melanogenesis level and related enzyme activity in melanoma cells, and shows a remarkable regulation effect at a CREB1 / MITF signal channel level. Mechanism research shows that the isorosmarinol and the isoasiaticoside down-regulate the CREB1 phosphorylation level and the expression of MITF through the synergistic effect of the isorosmarinol and the isoasiaticoside at an upstream signal regulation node, so that the overall inhibition of melanogenesis is realized. The composition disclosed by the invention has the characteristics of clear action mechanism, stable inhibition effect and good safety, and can be used for preparing cosmetics for whitening, fading spots and uniformizing skin color, external skin preparations or products for preventing and treating pigmentation-related skin problems.

The application provides a method for preparing vanillin by using eugenol, and belongs to the technical field of organic synthesis, and comprises the following steps: S1, preparing Ni-Mo / aluminosilicate / carbon-based material; S2, preparing isoeugenol; S3, mixing isoeugenol, the Ni-Mo / aluminosilicate / carbon-based material, anhydrous ethanol and deionized water in a closed pressure-resistant reaction kettle, adding sodium hydroxide solution dropwise, stirring, heating, passing in oxygen, stirring and reacting, cooling, separating, adjusting pH to acidity, aging, suction filtering, washing, adding into an ethanol aqueous solution, heating and stirring, decoloring, filtering, recrystallizing, washing, and vacuum drying to obtain vanillin. The application can effectively inhibit the occurrence of side reactions, and improve the yield and purity of the target product.

The present application discloses an application of isoeugenol in the preparation of an inhibitor for preventing and controlling pear fire blight disease and the preparation of isoeugenol, which relates to the field of biotechnology. By preparing a crude extract of Streptomyces sp.D67 to verify its activity against purple bacillus and amylovora pathogens, and verifying the active substances of the metabolites, it was determined that the metabolite isoeugenol has an inhibitory effect on amylovora pathogens. Isoeugenol is used in the preparation of inhibitors for preventing and controlling amylovora blight, with an effective concentration of 0.125mg / mL-0.5mg / mL. It has an inhibition rate of 44% on biofilm formation and 27% on extracellular polysaccharide formation. Isoeugenol can effectively inhibit the expression of virulence factors of amylovora blight pathogens, thereby reducing the drug resistance of amylovora blight pathogens and alleviating the occurrence of plant diseases and infection of plants. Spread effect.

The present invention discloses a mutant of isoeugenol dioxygenase, a preparation method and an application thereof. The mutant has at least one mutation at positions 216, 281, 283 and 307 in the amino acid sequence shown in SEQ ID NO. 1. Through reasonable design of isoeugenol dioxygenase, the finally obtained mutant has a high yield, realizes the efficient synthesis of high-value spice vanillin from cheap isoeugenol, and lays a foundation for the industrial synthesis of vanillin.

The invention provides a green, low-cost and solvent-free method for synthesizing isoeugenol, which comprises the following steps: heating and stirring a catalyst base and a phase transfer catalyst at a certain temperature, so that the catalyst base is completely dissolved in the phase transfer catalyst to form a complex; the method comprises the following steps: under a nitrogen protection environment, dropwise adding eugenol into a mixture of a catalyst alkali and a phase transfer catalyst, heating for reaction, and adjusting the pH value after the reaction to obtain a head product; and extracting the initial product with ethyl acetate, and then carrying out rotary evaporation to obtain isoeugenol. The phase transfer catalyst can dissolve eugenol and catalyst base at the same time, so that on one hand, a homogeneous catalysis environment is provided, and the catalysis efficiency is improved; on the other hand, anions are exposed after the catalyst alkali enters the phase transfer catalyst, so that the alkalinity of the alkali is enhanced; therefore, reaction conditions become mild, and a very high conversion rate is obtained.

The invention discloses a method for producing isoeugenol vanillin by using amycolatopsis, particularly relates to the technical field of microbial fermentation, and relates to a method for producing isoeugenol vanillin by using amycolatopsis. The isoeugenol vanillin produced by using the amycolatopsis is prepared from a recombinant strain and an isoeugenol solution; the recombinant strain is prepared from a recombinant plasmid and amycolatopsis; according to the invention, gene knockout is carried out at the position of a vanillin dehydrogenase gene, and isoeugenol monooxygenase is recombined and introduced to construct a recombinant gene, so that the target of efficiently converting isoeugenol into vanillin is achieved; according to the method, the amycolatopsis sp. With non-toxic substances and low vanillin degradation rate is adopted as a platform, and the isoeugenol monooxygenase is integrated into the genome of the amycolatopsis sp. Strain to construct a microbial synthesis chassis for high-yield isoeugenol vanillin, so that the green biosynthesis industrial production of vanillin is realized, and the cost is reduced.

The application discloses a vanillyl alcohol oxidase mutant, a preparation method and application thereof, wherein the wild-type vanillyl alcohol oxidase is from Penicillium simplicissimum Penicillium simplicissimum The activity center of the wild-type vanillyl alcohol oxidase is reformed by using a directed evolution method, and a vanillyl alcohol oxidase mutant with higher activity, cis-trans selectivity and cis-isoeugenol yield is obtained, wherein the mutant is at least one of S106L, L316F or I468L. The application realizes efficient synthesis of cis-isoeugenol from cheap 4-propyl guaiacol, and is beneficial to industrialized synthesis and application of cis-isoeugenol.

The invention relates to the technical field of metal surface treatment, in particular to a high-strength steel pipe based on hot-dip galvanizing and a preparation process thereof. The method comprises the following steps: firstly, carrying out hot-dip galvanizing treatment on a steel pipe, and then spraying a passivating agent; the passivating agent is obtained by compounding a composite coating, an amine curing agent and water, in the composite coating, the epoxy modified acrylic resin is formed by polymerizing methyl methacrylate, 4-epoxy isoeugenol and butyl acrylate, and the silicone oil modified epoxy resin is obtained by reacting hydroxyl silicone oil with isophorone diisocyanate to generate isocyanate-terminated silicone oil and then reacting an isocyanate group with hydroxyl in the epoxy resin; under the action of the amine curing agent, the epoxy group modified acrylic resin and the silicone oil modified epoxy resin are crosslinked, so that a compact protective layer is formed. The process can effectively protect the hot-dip galvanized steel pipe from being corroded, and the service life of the steel pipe is prolonged.

The invention relates to a novel epoxy resin based on isoeugenol. The object of the invention is to synthesize plastics, in particular epoxy resins, fiber-reinforced plastics, etc., based on renewable resources, especially for cabin construction in the aerospace industry, with a significantly increased bio-content, whereby the highest possible glass transition temperatures and a high crosslinking density are to be achieved. This object is achieved according to the invention by a bio-based epoxy resin monomer called triglycidyl isoeugenol (3EPO-IEU) according to claim 1 and a byproduct called diglycidyl allyl isoeugenol (2EPO-rA-IEU) according to claim 2. Furthermore, the object is achieved by a process according to claim 3 for the synthesis of triglycidyl isoeugenol from a provided solution, suspension, or emulsion of isoeugenol, whereby the also usable byproduct is formed. The product and the byproduct can be used alone or as a mixture.