16 results about "Isoeugenol" patented technology

The invention discloses an application of an isoeugenol monooxygenase mutant in production of isoeugenol vanillin, which comprises the steps of recombinant strain construction, fermentation culture of recombinant strains and catalytic reaction of fermentation broth, and the isoeugenol monooxygenase mutant is mutated through an isoeugenol monooxygenase amino acid sequence of wild fungi WT. And the thalli are expressed by escherichia coli, so that a series of thalli with a promoting effect on the catalytic efficiency of vanillin production are formed. The mutant super-efficient enzyme catalyst with the catalytic conversion rate reaching up to 98.5% or above is obtained through screening, the defects of low efficiency, pollution, high energy consumption and the like of traditional chemical catalyst production are overcome, and the mutant super-efficient enzyme catalyst is more suitable for green industrial production requirements and worthy of being popularized to industrial use.

The application discloses 4-propyl guaiacol oxidase mutants, a preparation method and application thereof, wherein wild-type 4-propyl guaiacol oxidase is from Penicillium simplicissimum Penicillium simplicissimum ​ The active center of the wild-type 4-propyl guaiacol oxidase is reformed by using a directed evolution method, and 4-propyl guaiacol oxidase mutants D170E, S426T, S50Q / D170E, D170E / L411V and D170E / L411V / S106C with high activity, cis-trans selectivity and trans-isoeugenol yield are obtained, efficient synthesis of high-value trans-isoeugenol from cheap 4-propyl guaiacol is realized, and the industrialized synthesis and application of the trans-isoeugenol are facilitated.

The application discloses a novel preparation for inhibiting botrytis cinerea and application thereof, and relates to the technical field of bacteriostatic drugs, and has the technical scheme as follows: the active ingredient of the preparation is composed of isoeugenol and bacillus velezensis fermentation liquor.The half bacteriostatic concentration EC50 value of the novel preparation reaches 59.7 ug / mL, and the bacteriostatic capacity is higher than that of the existing technology of bacto in the same concentration.Based on the influence on the membrane permeability of filamentous fungi, the application can synergistically inhibit the pathogenic activity of postharvest fungi of fruits, and is expected to be used for preparing a novel preparation for preventing and treating high-sugar fruits after harvesting.The application is simple to use, environment-friendly, can be directly smeared on the surface of fruits in the planting stage, can not be directly contacted with the fruits in the storage period, and can provide the bacteriostatic capacity for the storage period by using the fumigation mode.The application provides a new choice for the biological prevention of fruits, and has good economic benefits and social benefits.

The application discloses a method for preparing vanillin by using ferulic acid as raw material, and belongs to the technical field of organic chemical synthesis, which uses ferulic acid as raw material, reduces the reaction of isomerization of eugenol into isoeugenol, avoids using a large amount of alkali solution, and has the characteristics of clean and environmental protection, simple process, less side reaction, high product yield, etc.; the method comprises the following steps: (1) taking ferulic acid, adding an alcohol solvent, ferric chloride and hydrogen peroxide, and reacting after heating; (2) concentrating and recovering the alcohol solvent from the reaction liquid obtained in the step (1); (3) adding an ester solvent and an alkali aqueous solution to the alcohol solvent obtained in the step (2) for washing, layering after standing, and collecting the ester solvent layer solution; (4) concentrating and recovering the ester solvent from the ester solvent layer solution obtained in the step (3), recrystallizing the obtained solid with an ethanol aqueous solution, performing suction filtration, washing with water, and drying to obtain a finished product.

The invention provides a method for preparing vanillin from eugenol, and belongs to the technical field of organic synthesis, and the method comprises the following steps: S1, preparing a Ni-Mo / aluminosilicate / carbon-based material; s2, preparing isoeugenol; s3, isoeugenol, the Ni-Mo / aluminosilicate / carbon-based material, absolute ethyl alcohol and deionized water are added into a closed pressure-resistant reaction kettle to be mixed, a sodium hydroxide solution is dropwise added to be stirred, the temperature is increased, oxygen is introduced, stirring reaction, cooling, separation, pH adjustment to be acidic, aging, suction filtration and washing are conducted, the mixture is added into an ethanol water solution to be heated and stirred, decoloration, filtration, recrystallization and washing are conducted, and the Ni-Mo / aluminosilicate / carbon-based material is obtained. And drying in vacuum to obtain vanillin. According to the invention, side reactions can be effectively inhibited, and the yield and purity of the target product are improved.

The invention provides a blood-sucking midge efficient repellent and a screening and identifying method thereof, and relates to the technical field of biology. According to the invention, an odor receptor gene FtaiOr1 which is highly expressed at the head of a female gnat is obtained through identification, and a transgenic drosophila melanogaster strain Or22ab- / -GAL4 which can be used for screening a blood-sucking midge repellent compound is constructed through heterologous expression of the gene in a drosophila melanogaster ab3A neuron; and UAS-FtaiOr1 (UAS-FtaiOr1). Six blood sucking midge repelling compounds (camphor, eudesmol, (+)-anisone, alpha-terpilenol, (S)-cis-verbenol and isoterpinene) are obtained by screening of the fruit fly strain. The compounds can be used independently or in a compounded manner, are friendly to non-target organisms and environment, can be directly applied to repelling and on-site prevention and control of blood sucking midges, and have important application value in the aspects of reducing midge bites and reducing the propagation risk of entomophilous diseases.

The invention provides a preparation method of 3-methoxy-4-hydroxybenzaldehyde, and belongs to the technical field of organic synthesis. The preparation method comprises the following steps: taking isoeugenol as an initial raw material, carrying out electron transfer with a photocatalyst under the irradiation of visible light, taking oxygen as an oxidizing agent to form epoxide, oxidizing propenyl into aldehyde group under the irradiation of visible light, and finally breaking the epoxide to form vanillin. The synthesis method disclosed by the invention is simple in steps, the initial raw material is isoeugenol which is cheap and easy to obtain, air can be used as an oxidant, strong oxidants such as ozone are not needed, the use of high-pollution and high-toxicity reagents is reduced, and the method is safe to operate and environment-friendly; the reaction conditions are mild, strict low-temperature conditions are not needed, the reaction time is short, and the method is suitable for industrial production.

The invention relates to an isoeugenol monooxygenase mutant and an application of the isoeugenol monooxygenase mutant in preparation of vanillin. In particular to an isoeugenol monooxygenase mutant, nucleic acid for coding the isoeugenol monooxygenase mutant, a recombinant expression vector containing the nucleic acid, a recombinant expression transformant containing the recombinant expression vector, an isoeugenol monooxygenase mutant catalyst and preparation of the isoeugenol monooxygenase mutant catalyst. The invention also discloses an application of the isoeugenol monooxygenase mutant or the isoeugenol monooxygenase mutant catalyst in preparation of vanillin by taking isoeugenol as a substrate. The specific activity of the mutant I59L / L470I is 1.32 times that of a parent, and the selectivity of the mutant I59L / L470I is 1.40 times that of the parent. The mutant enzyme can catalyze propenyl on a benzene ring of a substrate isoeugenol so as to generate vanillin, the isoeugenol can be used as the substrate, and the isoeugenol can be completely converted to synthesize vanillin under the conditions that the reaction temperature is 25 DEG C, the pH value is 10.0, the cell quantity is 20g / L and the conversion time is 8 hours. And the conversion rate can reach 100% under the condition that the substrate concentration is 100mM, so that the method has a wide application prospect.

The application provides a continuous flow synthesis method of isopinocamphe 4,8-epoxide ‑ The reaction system has the advantages of less OH, weaker alkalinity, moderate oxidizability, mild reaction condition, directional oxidation of the double bond at the 4th position, high oxidation selectivity, and the selectivity of the target product, isopinocamphe 4,8-epoxide, can reach more than 90%. Meanwhile, the application overcomes the defect that the presence of water in the existing catalytic system can easily lead to ring-opening hydrolysis of the epoxidation reaction, greatly accelerates the reaction process of the epoxidation reaction, increases the yield of the product, and the yield can reach more than 84%. The reaction system has the advantages of mild reaction system condition, high oxidation selectivity, simple product separation, less side reaction, and easy treatment and recovery of waste liquid. The raw material conversion rate of the method is high, the selectivity and yield of the target product, isopinocamphe 4,8-epoxide, are high, the method is easy to realize industrialization, and is friendly to the environment.

The application discloses application of isoeugenol monooxygenase mutants in isoeugenol vanillin production, and comprises recombinant strain construction, fermentation culture of the recombinant strain and catalytic reaction of a fermentation liquor, wherein the isoeugenol monooxygenase mutants are obtained by mutating isoeugenol monooxygenase amino acid sequences of wild-type bacteria WT and expressing the obtained bacterial bodies by using Escherichia coli, and a series of bacterial bodies which promote catalytic efficiency of vanillin production are formed. The application screens mutant super-high-efficiency enzyme catalysts with a catalytic conversion rate of 98.5% or above, avoids the shortcomings of traditional chemical catalyst production, such as low efficiency, pollution and high energy consumption, and makes the application more suitable for green industrial production requirements, so that the application is worthy of popularization and industrial use.

The invention belongs to the technical field of dermatobiology and functional cosmetics / skin medicines, and particularly relates to a composition for synergistically inhibiting melanogenesis through isorosmarinol and isoasiaticoside and application of the composition. The composition in the present application contains isorosmarinol and isoasiaticoside as active ingredients. Experiments prove that the composition can reduce the melanogenesis level and related enzyme activity in melanoma cells, and shows a remarkable regulation effect at a CREB1 / MITF signal channel level. Mechanism research shows that the isorosmarinol and the isoasiaticoside down-regulate the CREB1 phosphorylation level and the expression of MITF through the synergistic effect of the isorosmarinol and the isoasiaticoside at an upstream signal regulation node, so that the overall inhibition of melanogenesis is realized. The composition disclosed by the invention has the characteristics of clear action mechanism, stable inhibition effect and good safety, and can be used for preparing cosmetics for whitening, fading spots and uniformizing skin color, external skin preparations or products for preventing and treating pigmentation-related skin problems.

The application provides a method for preparing vanillin by using eugenol, and belongs to the technical field of organic synthesis, and comprises the following steps: S1, preparing Ni-Mo / aluminosilicate / carbon-based material; S2, preparing isoeugenol; S3, mixing isoeugenol, the Ni-Mo / aluminosilicate / carbon-based material, anhydrous ethanol and deionized water in a closed pressure-resistant reaction kettle, adding sodium hydroxide solution dropwise, stirring, heating, passing in oxygen, stirring and reacting, cooling, separating, adjusting pH to acidity, aging, suction filtering, washing, adding into an ethanol aqueous solution, heating and stirring, decoloring, filtering, recrystallizing, washing, and vacuum drying to obtain vanillin. The application can effectively inhibit the occurrence of side reactions, and improve the yield and purity of the target product.

The present invention discloses a mutant of isoeugenol dioxygenase, a preparation method and an application thereof. The mutant has at least one mutation at positions 216, 281, 283 and 307 in the amino acid sequence shown in SEQ ID NO. 1. Through reasonable design of isoeugenol dioxygenase, the finally obtained mutant has a high yield, realizes the efficient synthesis of high-value spice vanillin from cheap isoeugenol, and lays a foundation for the industrial synthesis of vanillin.

The invention provides a green, low-cost and solvent-free method for synthesizing isoeugenol, which comprises the following steps: heating and stirring a catalyst base and a phase transfer catalyst at a certain temperature, so that the catalyst base is completely dissolved in the phase transfer catalyst to form a complex; the method comprises the following steps: under a nitrogen protection environment, dropwise adding eugenol into a mixture of a catalyst alkali and a phase transfer catalyst, heating for reaction, and adjusting the pH value after the reaction to obtain a head product; and extracting the initial product with ethyl acetate, and then carrying out rotary evaporation to obtain isoeugenol. The phase transfer catalyst can dissolve eugenol and catalyst base at the same time, so that on one hand, a homogeneous catalysis environment is provided, and the catalysis efficiency is improved; on the other hand, anions are exposed after the catalyst alkali enters the phase transfer catalyst, so that the alkalinity of the alkali is enhanced; therefore, reaction conditions become mild, and a very high conversion rate is obtained.

The application discloses a vanillyl alcohol oxidase mutant, a preparation method and application thereof, wherein the wild-type vanillyl alcohol oxidase is from Penicillium simplicissimum Penicillium simplicissimum The activity center of the wild-type vanillyl alcohol oxidase is reformed by using a directed evolution method, and a vanillyl alcohol oxidase mutant with higher activity, cis-trans selectivity and cis-isoeugenol yield is obtained, wherein the mutant is at least one of S106L, L316F or I468L. The application realizes efficient synthesis of cis-isoeugenol from cheap 4-propyl guaiacol, and is beneficial to industrialized synthesis and application of cis-isoeugenol.

The invention relates to a novel epoxy resin based on isoeugenol. The object of the invention is to synthesize plastics, in particular epoxy resins, fiber-reinforced plastics, etc., based on renewable resources, especially for cabin construction in the aerospace industry, with a significantly increased bio-content, whereby the highest possible glass transition temperatures and a high crosslinking density are to be achieved. This object is achieved according to the invention by a bio-based epoxy resin monomer called triglycidyl isoeugenol (3EPO-IEU) according to claim 1 and a byproduct called diglycidyl allyl isoeugenol (2EPO-rA-IEU) according to claim 2. Furthermore, the object is achieved by a process according to claim 3 for the synthesis of triglycidyl isoeugenol from a provided solution, suspension, or emulsion of isoeugenol, whereby the also usable byproduct is formed. The product and the byproduct can be used alone or as a mixture.