14 results about "Vanillyl alcohol" patented technology

The invention discloses vanillyl butyl ether and a synthesis method thereof, and relates to the technical field of synthesis of vanillyl butyl ether.The synthesis method of vanillyl butyl ether comprises the following steps that S1, vanillyl alcohol is prepared; s2, adding vanillyl alcohol prepared in the step S1, n-butyl alcohol and a catalyst into a reaction kettle, and uniformly stirring to react, so as to obtain a mixed solution after the reaction is completed; s3, filtering the mixed solution prepared in S2, pretreating filtrate, and extracting an organic phase in the filtrate; s4, carrying out primary reduced pressure distillation on the organic phase extracted in S3 to obtain a vanillyl butyl ether crude product and n-butyl alcohol, then recovering n-butyl alcohol, and carrying out secondary reduced pressure distillation on the vanillyl butyl ether crude product to obtain vanillyl butyl ether; the whole synthesis method is low in raw material cost, mild in reaction condition, simple in process and small in wastewater generation amount, recycling of the catalyst and the solvent is achieved, the prepared vanillyl butyl ether is large in yield and high in purity, a brand new solution is provided for efficient and green production of the vanillyl butyl ether, and industrial popularization is facilitated.

The invention provides recombinant escherichia coli for synthesizing vanillyl alcohol as well as a construction method and application of the recombinant escherichia coli. Wherein the recombinant escherichia coli comprises a host bacterium, and the host bacterium overexpresses a DAHP synthetase encoding gene AroGfbr and a 3-dehydroshikimic acid dehydratase encoding gene QuiC on an escherichia coli genome by using a pTrc promoter, so that sufficient supply of protocatechuic acid is realized; an alcohol dehydrogenase encoding gene ADH4 or ADH6 is introduced, so that the conversion speed of a toxic intermediate 3, 4-dihydroxybenzaldehyde is accelerated, and the fermentation density of the strain is improved; genes mtn and luxs of a methylation cofactor SAM regeneration pathway are over-expressed on a genome, so that the methylation efficiency is improved. Therefore, the recombinant Escherichia coli provided by the invention can efficiently synthesize vanillyl alcohol through the combination of the transformation strategies, and can efficiently accumulate 1.66 g / L vanillyl alcohol in a shake flask, and the yield of a 3L fermentation tank reaches 6.21 g / L. The method for producing vanillyl alcohol by using the recombinant strain provided by the invention has the advantages of low cost, controllable process, simplicity and convenience in separation and extraction, and facilitation of industrial production.

The invention relates to a bio-based benzoxazine monomer as well as a preparation method and application thereof, and the bio-based benzoxazine monomer is obtained by reaction of 5, 5 '-methylene difurfuryl amine, vanillyl alcohol and paraformaldehyde. The content ratio of the monomer biomass is high, and the resin obtained by melting and curing the monomer biomass is good in heat resistance, high in strength and good in toughness. More importantly, the difference value between the melting temperature and the curing temperature of the monomer is large, the monomer can be copolymerized with a Schiff base phenolic monomer obtained by reacting vanillin with tyramine to prepare the degradable benzoxazine copolymer resin with high biomass content, and the copolymer has outstanding heat resistance, flame retardance, mechanical properties and the like, and is suitable for industrial production. The problems of low biomass content and low heat resistance of degradable biomass benzoxazine resin in the prior art are solved, and the degradable biomass benzoxazine resin has good application prospects in the fields of adhesives, coatings and the like.

The invention relates to the technical field of biological medicine, in particular to application of vanillyl alcohol in preparation of a dopamine receptor D2 antagonist, and the application proves that the vanillyl alcohol can be specifically combined with a D2 receptor with micromole-level affinity through micro-thermophoresis and biological layer interference technologies. Cell experiments show that vanillyl alcohol can antagonize D2 receptor mediated ERK signal pathway activation in a concentration-dependent manner, and has no agonist activity. In a PCPA-induced insomnia mouse model, vanillyl alcohol can shorten the sleep latency and prolong the total sleep time in a dose-dependent manner. After a D2 receptor is knocked down in nucleus septum, the sleep promoting effect of vanillyl alcohol disappears, and it is confirmed that the drug effect of vanillyl alcohol has target dependence. As a low-toxicity natural small molecule, vanillyl alcohol provides a new choice for developing a novel insomnia treatment medicine.

The utility model relates to the technical field of reaction devices, in particular to a vanillin alcohol and n-butyl alcohol etherification reaction catalytic device, materials are put into a feed hopper assembly, the feed hopper assembly has an anti-blocking function, so that the materials quickly enter a tank body, a stirring assembly is started, the materials are quickly mixed and reacted, and in the reaction process, the materials can be quickly separated from the tank body. The tank body is cooled by the cooling assembly, so that the reaction efficiency is improved; comprising a tank body, supporting legs and bases, a plurality of sets of supporting legs are arranged at the bottom end of the tank body, a set of bases is arranged at the bottom end of each set of supporting legs, the cooling assembly is arranged on the outer side wall of the tank body, the stirring assembly is arranged in the tank body, and the feeding hopper assembly is arranged at the input end of the tank body.

The application discloses a non-catalyst electrolytic reduction method for preparing vanillin alcohol from vanillin, belonging to the non-metal electrolysis field, which comprises the following steps: dissolving vanillin in a solvent to obtain an electrolyte; the solvent is selected from 0.05-0.15 M sulfuric acid aqueous solution or a mixed solvent of 0.05-0.15 M sulfuric acid aqueous solution and alcohol solvent; and the electrolyte is placed in a single-chamber electrolytic cell equipped with an anode and a cathode to perform electrolysis, so that vanillin is hydrogenated and reduced into vanillin alcohol; wherein the cathode is Fe, and the anode is selected from Fe or Al. The application has excellent performance for preparing vanillin alcohol from vanillin through hydrogenation reaction, under the optimal conditions, the conversion rate of vanillin can reach 91.8%, and the selectivity of vanillin alcohol can reach 97.7%, which is higher than that of the existing catalyst; the device used is simple, no additional hydrogen gas is needed, no catalyst is needed, the reaction can be carried out at normal temperature, the reaction condition is mild, and the energy consumption cost is low.

The invention provides a method for constructing and screening a methyltransferase mutant library and application of the methyltransferase mutant library in biosynthesis. The 3, 4-dihydroxybenzyl alcohol (3, 4-DHBA) is a direct precursor for biosynthesis of a plant natural product vanillyl alcohol by using microorganisms. Through molecular docking analysis, the interaction force difference between the enzyme to be designed and caffeic acid and 3, 4-DHBA in a catalytic pocket is compared, mutation hot spot amino acid residues are determined, and a methyltransferase saturated mutation library is constructed based on the mutation hot spot amino acid residues. On the basis that consumption of a methylation reaction substrate is visually detected by taking FeCl3 as an indicator, a high-throughput screening method for the methyltransferase mutant is constructed, and the mutant V314I with high catalytic activity on 3, 4-DHBA is obtained through screening. The mutant V3141 is obtained by carrying out V314I mutation on an amino acid sequence as shown in SEQ ID NO. 1. The mutant V3141 is a mutant V3141. Compared with a wild type, the mutant V314I has the advantages that kcat / Km is increased by 95%, the yield of vanillyl alcohol in a shake flask addition experiment is increased by 47%, and the mutant provides a certain basis for efficiently synthesizing vanillyl alcohol and derivatives thereof by utilizing microorganisms.

A method of making a flame retardant, and phosphorylated bio-based flame retardants, are provided. The method includes reacting a bio-based-derived starting compound with a phosphate-containing compound or o-phosphoric acid to obtain a phosphorylated bio-based material. The phosphate-containing compound may be diphenyl phosphoryl chloride. The obtained phosphorylated bio-based material may be phosphorylated cardanol, vanillin diphenyl phosphate, vanillyl alcohol diphenyl phosphate, methyl gallate triphenyl phosphate, bis(dihydrogenphosphate) of vanillyl alcohol diglicidyl ether, or bis(dihydrogenphosphate) of bisphenol A diglicidyl ether. An article including the phosphorylated bio-based flame retardant is also provided. The article may be a building material, a textile, a plastic, a foam, an adhesive, packaging, and an electrical housing.

The invention provides a vinasse carbon supported palladium catalyst for catalyzing vanillin hydrogenation as well as a preparation method and application thereof, and relates to the technical field of catalysts. The preparation method of the vinasse carbon supported palladium catalyst comprises the following steps: carrying out carbonization treatment on vinasse in a protective atmosphere, then soaking the carbonized vinasse in a solution of an activating agent of charcoal, drying the carbonized vinasse, and then carrying out activating treatment in the protective atmosphere to prepare a vinasse carbon carrier; mixing the vinasse carbon carrier with a solution of a divalent palladium salt, and carrying out solid-liquid separation to obtain a vinasse carbon carrier loaded with the divalent palladium salt; and dispersing the vinasse carbon carrier loaded with the divalent palladium salt in water, and then adding a reducing agent to reduce divalent palladium to prepare the vinasse carbon loaded palladium catalyst. The preparation method is simple and convenient, the prepared vinasse carbon supported palladium catalyst is used for catalyzing selective hydrogenation of vanillin to prepare vanillyl alcohol, the selectivity of vanillyl alcohol reaches up to 100%, the yield is high, and the catalyst is stable in structure and can be repeatedly used.

The application discloses a kind of based on lignin's vanillyl alcohol group polyester carbon nanotube lithium ion battery negative electrode material and preparation method thereof, the negative electrode material includes carbon nanotube and vanillyl alcohol group polyester coating layer in situ grown on carbon nanotube;Vanillyl alcohol group polyester coating layer is obtained by in-situ polycondensation reaction of vanillyl alcohol and terephthaloyl chloride.The organic polymer negative electrode material of the application uses lignin-derived vanillyl alcohol and terephthaloyl chloride as polycondensation monomer, and constructs vanillyl alcohol group polyester coating layer on the surface of carbon nanotube by in-situ polycondensation reaction, to form the stable structure and the interface of vanillyl alcohol group polyester carbon nanotube composite structure that is closely combined, compared with simple physical mixing, in-situ growth is more advantageous to improve electron transport efficiency and the utilization rate of active site, so that the lithium ion battery assembled has good cycle stability, rate performance, higher electrochemical reversibility and lower charge transfer impedance.

The invention provides a genetically engineered bacterium for synthesizing vanillyl alcohol by taking glucose as a substrate and application of the genetically engineered bacterium, the genetically engineered bacterium comprises a protocatechuic acid synthesis module and a vanillyl alcohol synthesis module, the protocatechuic acid synthesis module expresses a 3-dehydroshikimic acid dehydratase gene, and the vanillyl alcohol synthesis module expresses a 3-dehydroshikimic acid dehydratase gene. The vanillyl alcohol synthesis module is used for expressing a carboxylic acid reductase gene, a phosphopantetheinyl transferase gene and a 3-O-methyltransferase gene. According to the genetically engineered bacterium constructed by the invention, glucose is used as a substrate to synthesize vanillyl alcohol, so that the synthesis step of vanillyl alcohol is shortened, no additional substrate is needed, the yield of vanillyl alcohol obtained by shake flask fermentation can reach 1145.94 mg / L, and the yield of vanillyl alcohol obtained by fed-batch fermentation can reach 1007.07 mg / L.

The invention discloses a method for preparing milk-flavor tobacco essence from tobacco leaf waste and application of the milk-flavor tobacco essence in cigarettes. The method comprises the following steps: (1) preparation of a Co-Pt micro-nano particle catalyst: dissolving CoCl2. 6H2O and PtCl2 in ethylene glycol, adding a surfactant PVP, and uniformly stirring; adding N2H4.H2O, stirring, sealing the mixed solution into a reaction kettle, keeping the temperature at 150-180 DEG C for 8-12 hours, cooling and collecting black powder; and washing with absolute ethyl alcohol and drying. (2) catalytic oxidation reaction: adding the tobacco leaf residues after supercritical extraction, a catalyst, 4-methyl guaiacol and / or vanillyl alcohol and an ethanol-water mixed solvent into a reaction kettle, introducing oxygen, pressurizing to 2-3MPa, stirring and heating to 160-180 DEG C, reacting for 4-12h, centrifuging turbid liquid, and taking supernatant liquid, namely the milk-flavor tobacco essence; and (3) catalyst recovery: drying, sieving and cleaning centrifuged solid residues, and recovering the catalyst. The equipment is simple, the preparation method is green and efficient, the sensory quality of the obtained absolute oil is outstanding, and the smoke state is obviously improved.

The application discloses a genetically engineered bacterium for producing cinnamic acid and derivatives thereof, a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium co-expresses vanillyl alcohol oxidase and oxidoreductase. The genetically engineered bacterium can be used as a biological catalyst to convert a substrate into cinnamic acid and derivatives thereof. The method for producing cinnamic acid and derivatives thereof has the advantages of high production efficiency, short reaction period, low cost and good industrial application prospect.

The invention provides a self-induction culture medium suitable for multi-plasmid co-expression recombinant bacteria and application of the self-induction culture medium. The self-induction culture medium is prepared from the following components: 0.6 to 1 g / L of glucose, 3 to 5 g / L of lactose, 5 to 15 g / L of glycerol, 5 to 15 g / L of yeast extract, 6.8 to 13.6 g / L of Na2HPO4, 3.0 to 6.0 g / L of KH2PO4, 0.5 to 10 g / L of NaCl, 1.0 to 3.0 g / L of NH4Cl, MgSO4 with the final concentration of 1 to 3 mol / L, CaCl2 with the final concentration of 0.1 to 2 mol / L, 0.15 to 10 g / L of methionine and 1 to 2 mg / L of vitamin B12. The self-induction culture medium is simple in component and convenient to operate, recombinant escherichia coli E.coli BL21 (DE3)-VA which simultaneously carries three recombinant expression vectors pETDuet-1, pCDFDuet-1 and pRSFDuet-1 and can express carboxylic acid reductase, methyltransferase and 3-dehydroshikimic acid dehydratase can be induced and cultured, the self-induction culture medium is used for fermentation production of vanillyl alcohol, the yield of vanillyl alcohol can reach 277.85 mg / L after fermentation is performed for 48 h, and the yield is 3.8 times that of an IPTG induction mode.