23 results about "Vanillyl alcohol" patented technology

The invention provides a compound feed additive capable of improving the flavor of eggs, a preparation technology and a compound feed, and relates to the technical field of feed preparation.Main components in shaddock peel are analyzed, the main components influencing the flavor of the eggs are screened out, and chemical components and shaddock peel powder are adopted to prepare the compound feed additive. The grapefruit flavor extract (active ingredients such as limonene, polyphenols and the like) prepared on the basis of the grapefruit peel powder. The preparation method comprises the following steps: compounding the raw materials in parts by weight: 20-40 parts of linalool, 5-10 parts of linalyl acetate, 2-4 parts of limonene, 1-2 parts of vanillyl alcohol, 0.7-1.4 parts of geranyl acetate, 5-10 parts of myrcene, 80 parts of pomelo peel powder, 150 parts of a carrier and 70 parts of a pomelo flavor extract; the total addition amount of the shaddock peel powder in the basal feed is reduced from 2% to 0.1%, a good flavor improvement effect can be maintained, and dependence on shaddock peel raw materials is greatly reduced.

The invention discloses vanillyl butyl ether and a synthesis method thereof, and relates to the technical field of synthesis of vanillyl butyl ether.The synthesis method of vanillyl butyl ether comprises the following steps that S1, vanillyl alcohol is prepared; s2, adding vanillyl alcohol prepared in the step S1, n-butyl alcohol and a catalyst into a reaction kettle, and uniformly stirring to react, so as to obtain a mixed solution after the reaction is completed; s3, filtering the mixed solution prepared in S2, pretreating filtrate, and extracting an organic phase in the filtrate; s4, carrying out primary reduced pressure distillation on the organic phase extracted in S3 to obtain a vanillyl butyl ether crude product and n-butyl alcohol, then recovering n-butyl alcohol, and carrying out secondary reduced pressure distillation on the vanillyl butyl ether crude product to obtain vanillyl butyl ether; the whole synthesis method is low in raw material cost, mild in reaction condition, simple in process and small in wastewater generation amount, recycling of the catalyst and the solvent is achieved, the prepared vanillyl butyl ether is large in yield and high in purity, a brand new solution is provided for efficient and green production of the vanillyl butyl ether, and industrial popularization is facilitated.

The invention provides an acidizing corrosion inhibitor as well as a preparation method, application and a use method thereof, and relates to the technical field of chemical additives, the acidizing corrosion inhibitor comprises vanillyl alcohol and lauryl trimethyl ammonium bromide, and further comprises one or two of piperazine-1, 4-bis-ethanesulfonic acid and polyoxazoline. All chemical components used in the formula are easy to obtain and low in price; vanillyl alcohol is derived from vanillin, is a bio-based aromatic compound, and is green and environment-friendly; the vanillyl alcohol, the lauryl trimethyl ammonium bromide, the piperazine-1, 4-bis-ethanesulfonic acid and the polyoxazoline are biodegradable chemical substances, are environment-friendly and non-toxic, and have no negative influence on the environment and human life; the acidizing corrosion inhibitor has good solubility in an acidizing corrosive solution, has stability and high efficiency at high temperature, and is still stable and very effective at the high temperature up to 150 DEG C.

The invention provides recombinant escherichia coli for synthesizing vanillyl alcohol as well as a construction method and application of the recombinant escherichia coli. Wherein the recombinant escherichia coli comprises a host bacterium, and the host bacterium overexpresses a DAHP synthetase encoding gene AroGfbr and a 3-dehydroshikimic acid dehydratase encoding gene QuiC on an escherichia coli genome by using a pTrc promoter, so that sufficient supply of protocatechuic acid is realized; an alcohol dehydrogenase encoding gene ADH4 or ADH6 is introduced, so that the conversion speed of a toxic intermediate 3, 4-dihydroxybenzaldehyde is accelerated, and the fermentation density of the strain is improved; genes mtn and luxs of a methylation cofactor SAM regeneration pathway are over-expressed on a genome, so that the methylation efficiency is improved. Therefore, the recombinant Escherichia coli provided by the invention can efficiently synthesize vanillyl alcohol through the combination of the transformation strategies, and can efficiently accumulate 1.66 g / L vanillyl alcohol in a shake flask, and the yield of a 3L fermentation tank reaches 6.21 g / L. The method for producing vanillyl alcohol by using the recombinant strain provided by the invention has the advantages of low cost, controllable process, simplicity and convenience in separation and extraction, and facilitation of industrial production.

The invention relates to a bio-based benzoxazine monomer as well as a preparation method and application thereof, and the bio-based benzoxazine monomer is obtained by reaction of 5, 5 '-methylene difurfuryl amine, vanillyl alcohol and paraformaldehyde. The content ratio of the monomer biomass is high, and the resin obtained by melting and curing the monomer biomass is good in heat resistance, high in strength and good in toughness. More importantly, the difference value between the melting temperature and the curing temperature of the monomer is large, the monomer can be copolymerized with a Schiff base phenolic monomer obtained by reacting vanillin with tyramine to prepare the degradable benzoxazine copolymer resin with high biomass content, and the copolymer has outstanding heat resistance, flame retardance, mechanical properties and the like, and is suitable for industrial production. The problems of low biomass content and low heat resistance of degradable biomass benzoxazine resin in the prior art are solved, and the degradable biomass benzoxazine resin has good application prospects in the fields of adhesives, coatings and the like.

The invention relates to the technical field of biological medicine, in particular to application of vanillyl alcohol in preparation of a dopamine receptor D2 antagonist, and the application proves that the vanillyl alcohol can be specifically combined with a D2 receptor with micromole-level affinity through micro-thermophoresis and biological layer interference technologies. Cell experiments show that vanillyl alcohol can antagonize D2 receptor mediated ERK signal pathway activation in a concentration-dependent manner, and has no agonist activity. In a PCPA-induced insomnia mouse model, vanillyl alcohol can shorten the sleep latency and prolong the total sleep time in a dose-dependent manner. After a D2 receptor is knocked down in nucleus septum, the sleep promoting effect of vanillyl alcohol disappears, and it is confirmed that the drug effect of vanillyl alcohol has target dependence. As a low-toxicity natural small molecule, vanillyl alcohol provides a new choice for developing a novel insomnia treatment medicine.

The utility model relates to the technical field of reaction devices, in particular to a vanillin alcohol and n-butyl alcohol etherification reaction catalytic device, materials are put into a feed hopper assembly, the feed hopper assembly has an anti-blocking function, so that the materials quickly enter a tank body, a stirring assembly is started, the materials are quickly mixed and reacted, and in the reaction process, the materials can be quickly separated from the tank body. The tank body is cooled by the cooling assembly, so that the reaction efficiency is improved; comprising a tank body, supporting legs and bases, a plurality of sets of supporting legs are arranged at the bottom end of the tank body, a set of bases is arranged at the bottom end of each set of supporting legs, the cooling assembly is arranged on the outer side wall of the tank body, the stirring assembly is arranged in the tank body, and the feeding hopper assembly is arranged at the input end of the tank body.

The application discloses a non-catalyst electrolytic reduction method for preparing vanillin alcohol from vanillin, belonging to the non-metal electrolysis field, which comprises the following steps: dissolving vanillin in a solvent to obtain an electrolyte; the solvent is selected from 0.05-0.15 M sulfuric acid aqueous solution or a mixed solvent of 0.05-0.15 M sulfuric acid aqueous solution and alcohol solvent; and the electrolyte is placed in a single-chamber electrolytic cell equipped with an anode and a cathode to perform electrolysis, so that vanillin is hydrogenated and reduced into vanillin alcohol; wherein the cathode is Fe, and the anode is selected from Fe or Al. The application has excellent performance for preparing vanillin alcohol from vanillin through hydrogenation reaction, under the optimal conditions, the conversion rate of vanillin can reach 91.8%, and the selectivity of vanillin alcohol can reach 97.7%, which is higher than that of the existing catalyst; the device used is simple, no additional hydrogen gas is needed, no catalyst is needed, the reaction can be carried out at normal temperature, the reaction condition is mild, and the energy consumption cost is low.

The invention provides a method for constructing and screening a methyltransferase mutant library and application of the methyltransferase mutant library in biosynthesis. The 3, 4-dihydroxybenzyl alcohol (3, 4-DHBA) is a direct precursor for biosynthesis of a plant natural product vanillyl alcohol by using microorganisms. Through molecular docking analysis, the interaction force difference between the enzyme to be designed and caffeic acid and 3, 4-DHBA in a catalytic pocket is compared, mutation hot spot amino acid residues are determined, and a methyltransferase saturated mutation library is constructed based on the mutation hot spot amino acid residues. On the basis that consumption of a methylation reaction substrate is visually detected by taking FeCl3 as an indicator, a high-throughput screening method for the methyltransferase mutant is constructed, and the mutant V314I with high catalytic activity on 3, 4-DHBA is obtained through screening. The mutant V3141 is obtained by carrying out V314I mutation on an amino acid sequence as shown in SEQ ID NO. 1. The mutant V3141 is a mutant V3141. Compared with a wild type, the mutant V314I has the advantages that kcat / Km is increased by 95%, the yield of vanillyl alcohol in a shake flask addition experiment is increased by 47%, and the mutant provides a certain basis for efficiently synthesizing vanillyl alcohol and derivatives thereof by utilizing microorganisms.

A method of making a flame retardant, and phosphorylated bio-based flame retardants, are provided. The method includes reacting a bio-based-derived starting compound with a phosphate-containing compound or o-phosphoric acid to obtain a phosphorylated bio-based material. The phosphate-containing compound may be diphenyl phosphoryl chloride. The obtained phosphorylated bio-based material may be phosphorylated cardanol, vanillin diphenyl phosphate, vanillyl alcohol diphenyl phosphate, methyl gallate triphenyl phosphate, bis(dihydrogenphosphate) of vanillyl alcohol diglicidyl ether, or bis(dihydrogenphosphate) of bisphenol A diglicidyl ether. An article including the phosphorylated bio-based flame retardant is also provided. The article may be a building material, a textile, a plastic, a foam, an adhesive, packaging, and an electrical housing.

A composition contains at least one of homovanillyl alcohol (HVA) or its previously unidentified isomer (isoHVA). Additionally or alternatively, the composition contains a precursor of HVA and / or isoHVA (e.g., hydroxytyrosol, oleuropein aglycone, oleuropein, demethyloleuropein, oleuroside, verbascoside or mixtures thereof) and also contains at least one probiotic and / or at least one enzyme which convert the precursor to HVA and / or isoHVA in vivo (and / or an intermediate compound then subjected to methylation in vivo to form HVA and / or isoHVA). The composition can be administered to an individual to treat or reduce incidence of impaired mobility in an older adult; stimulate bone formation and / or inhibit bone resorption; treat or reduce incidence of synovitis in an individual in need or at risk thereof; treat or reduce incidence of articular cartilage degradation subsequent to synovitis in an individual having or recovering from synovitis; or treat or reduce incidence of cartilage breakdown.

The invention provides a vinasse carbon supported palladium catalyst for catalyzing vanillin hydrogenation as well as a preparation method and application thereof, and relates to the technical field of catalysts. The preparation method of the vinasse carbon supported palladium catalyst comprises the following steps: carrying out carbonization treatment on vinasse in a protective atmosphere, then soaking the carbonized vinasse in a solution of an activating agent of charcoal, drying the carbonized vinasse, and then carrying out activating treatment in the protective atmosphere to prepare a vinasse carbon carrier; mixing the vinasse carbon carrier with a solution of a divalent palladium salt, and carrying out solid-liquid separation to obtain a vinasse carbon carrier loaded with the divalent palladium salt; and dispersing the vinasse carbon carrier loaded with the divalent palladium salt in water, and then adding a reducing agent to reduce divalent palladium to prepare the vinasse carbon loaded palladium catalyst. The preparation method is simple and convenient, the prepared vinasse carbon supported palladium catalyst is used for catalyzing selective hydrogenation of vanillin to prepare vanillyl alcohol, the selectivity of vanillyl alcohol reaches up to 100%, the yield is high, and the catalyst is stable in structure and can be repeatedly used.

The invention relates to a stable danazol capsule and a preparation method thereof, and belongs to the technical field of pharmaceutical preparations for treating endocrine system diseases. The danazol capsule disclosed by the invention is prepared from danazol, vanillin, a pH-regulated hydroxypropyl starch-glycerol matrix, a filling agent and a lubricating agent. The danazol and vanillin are co-ground, a hydroxypropyl starch-glycerol matrix with a specific pH value is combined, and a reasonable filling agent, a lubricant and a preparation process are matched, so that the danazol tablet is prepared, the problems of poor stability and low dissolution rate of an existing danazol preparation are solved, the raw materials are easy to obtain, the preparation process is simple, and industrial production is facilitated.

The invention discloses recombinant escherichia coli for producing coniferyl alcohol by taking eugenol as a substrate and application of the recombinant escherichia coli, and belongs to the technical field of microbial fermentation. The recombinant escherichia coli heterologously expresses a key enzyme gene for converting eugenol into coniferyl alcohol, and the protein coded by the key gene is vanillyl alcohol oxidase. The recombinant escherichia coli can produce coniferyl alcohol through shake flask fermentation with eugenol as a substrate, after fermentation culture of the recombinant escherichia coli, the concentration of the coniferyl alcohol can reach 23.17 g / L through fermentation in 50 mL of an M9YE culture medium, the conversion rate is 92.68%, and the yield of the coniferyl alcohol is remarkably higher than that of other recombinant strains of the same type.

The application discloses a vanillyl alcohol oxidase mutant, a preparation method and application thereof, wherein the wild-type vanillyl alcohol oxidase is from Penicillium simplicissimum Penicillium simplicissimum The activity center of the wild-type vanillyl alcohol oxidase is reformed by using a directed evolution method, and a vanillyl alcohol oxidase mutant with higher activity, cis-trans selectivity and cis-isoeugenol yield is obtained, wherein the mutant is at least one of S106L, L316F or I468L. The application realizes efficient synthesis of cis-isoeugenol from cheap 4-propyl guaiacol, and is beneficial to industrialized synthesis and application of cis-isoeugenol.

The application discloses a kind of based on lignin's vanillyl alcohol group polyester carbon nanotube lithium ion battery negative electrode material and preparation method thereof, the negative electrode material includes carbon nanotube and vanillyl alcohol group polyester coating layer in situ grown on carbon nanotube;Vanillyl alcohol group polyester coating layer is obtained by in-situ polycondensation reaction of vanillyl alcohol and terephthaloyl chloride.The organic polymer negative electrode material of the application uses lignin-derived vanillyl alcohol and terephthaloyl chloride as polycondensation monomer, and constructs vanillyl alcohol group polyester coating layer on the surface of carbon nanotube by in-situ polycondensation reaction, to form the stable structure and the interface of vanillyl alcohol group polyester carbon nanotube composite structure that is closely combined, compared with simple physical mixing, in-situ growth is more advantageous to improve electron transport efficiency and the utilization rate of active site, so that the lithium ion battery assembled has good cycle stability, rate performance, higher electrochemical reversibility and lower charge transfer impedance.

The invention provides a method for producing sesamin based on recombinant Escherichia coli-saccharomycetes step-by-step fermentation, and relates to the technical field of bioengineering.The recombinant Escherichia coli heterologously expresses a key enzyme gene for converting eugenol into coniferyl alcohol, and protein coded by the key gene is vanillyl alcohol oxidase. The cheap chemical raw material eugenol can be converted into coniferyl alcohol; the recombinant saccharomyces cerevisiae strain expresses a key enzyme for synthesizing sesamin, namely sesamin synthase CYP81Q1 and guide protein DIR, fermentation liquor of recombinant escherichia coli can be converted into sesamin, 79 mg / L of sesamin is synthesized, and the production cost of sesamin is reduced.

The invention provides a genetically engineered bacterium for synthesizing vanillyl alcohol by taking glucose as a substrate and application of the genetically engineered bacterium, the genetically engineered bacterium comprises a protocatechuic acid synthesis module and a vanillyl alcohol synthesis module, the protocatechuic acid synthesis module expresses a 3-dehydroshikimic acid dehydratase gene, and the vanillyl alcohol synthesis module expresses a 3-dehydroshikimic acid dehydratase gene. The vanillyl alcohol synthesis module is used for expressing a carboxylic acid reductase gene, a phosphopantetheinyl transferase gene and a 3-O-methyltransferase gene. According to the genetically engineered bacterium constructed by the invention, glucose is used as a substrate to synthesize vanillyl alcohol, so that the synthesis step of vanillyl alcohol is shortened, no additional substrate is needed, the yield of vanillyl alcohol obtained by shake flask fermentation can reach 1145.94 mg / L, and the yield of vanillyl alcohol obtained by fed-batch fermentation can reach 1007.07 mg / L.

The invention discloses a method for preparing milk-flavor tobacco essence from tobacco leaf waste and application of the milk-flavor tobacco essence in cigarettes. The method comprises the following steps: (1) preparation of a Co-Pt micro-nano particle catalyst: dissolving CoCl2. 6H2O and PtCl2 in ethylene glycol, adding a surfactant PVP, and uniformly stirring; adding N2H4.H2O, stirring, sealing the mixed solution into a reaction kettle, keeping the temperature at 150-180 DEG C for 8-12 hours, cooling and collecting black powder; and washing with absolute ethyl alcohol and drying. (2) catalytic oxidation reaction: adding the tobacco leaf residues after supercritical extraction, a catalyst, 4-methyl guaiacol and / or vanillyl alcohol and an ethanol-water mixed solvent into a reaction kettle, introducing oxygen, pressurizing to 2-3MPa, stirring and heating to 160-180 DEG C, reacting for 4-12h, centrifuging turbid liquid, and taking supernatant liquid, namely the milk-flavor tobacco essence; and (3) catalyst recovery: drying, sieving and cleaning centrifuged solid residues, and recovering the catalyst. The equipment is simple, the preparation method is green and efficient, the sensory quality of the obtained absolute oil is outstanding, and the smoke state is obviously improved.

The application discloses a genetically engineered bacterium for producing cinnamic acid and derivatives thereof, a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium co-expresses vanillyl alcohol oxidase and oxidoreductase. The genetically engineered bacterium can be used as a biological catalyst to convert a substrate into cinnamic acid and derivatives thereof. The method for producing cinnamic acid and derivatives thereof has the advantages of high production efficiency, short reaction period, low cost and good industrial application prospect.

The application discloses a bio-based flame-retardant polyurethane soft foam with photo-thermal conversion performance and a preparation method and application thereof, and comprises the following steps: reacting vanillyl alcohol (VA) with phenyl phosphorodichloridate (PPDC) to synthesize vanillyl alcohol-based phosphorus-containing dihydric alcohol, i.e., a flame-retardant polyol (PPDVA); uniformly mixing castor oil as a biomass material with the flame-retardant polyol, polytetrahydrofuran, MXene (Ti3C2T x ) powder, a foaming agent, a catalyst and a foam stabilizer to prepare a component A precursor solution; adding a component B isocyanate into the component A; and obtaining a polyurethane soft foam loaded with Ti3C2T x after foaming and curing; and immersing the polyurethane soft foam loaded with Ti3C2T x in a PDMS solution and performing ultrasonic treatment, and then solidifying in an oven to obtain the bio-based flame-retardant polyurethane soft foam with photo-thermal conversion performance. The polyurethane soft foam obtained by the application has superhydrophobicity, good photo-thermal conversion performance and good flame-retardant effect, and can be applied to the fields of oil-water separation and flame retardation; and the preparation method is simple, green, environment-friendly and the like.

The invention provides a self-induction culture medium suitable for multi-plasmid co-expression recombinant bacteria and application of the self-induction culture medium. The self-induction culture medium is prepared from the following components: 0.6 to 1 g / L of glucose, 3 to 5 g / L of lactose, 5 to 15 g / L of glycerol, 5 to 15 g / L of yeast extract, 6.8 to 13.6 g / L of Na2HPO4, 3.0 to 6.0 g / L of KH2PO4, 0.5 to 10 g / L of NaCl, 1.0 to 3.0 g / L of NH4Cl, MgSO4 with the final concentration of 1 to 3 mol / L, CaCl2 with the final concentration of 0.1 to 2 mol / L, 0.15 to 10 g / L of methionine and 1 to 2 mg / L of vitamin B12. The self-induction culture medium is simple in component and convenient to operate, recombinant escherichia coli E.coli BL21 (DE3)-VA which simultaneously carries three recombinant expression vectors pETDuet-1, pCDFDuet-1 and pRSFDuet-1 and can express carboxylic acid reductase, methyltransferase and 3-dehydroshikimic acid dehydratase can be induced and cultured, the self-induction culture medium is used for fermentation production of vanillyl alcohol, the yield of vanillyl alcohol can reach 277.85 mg / L after fermentation is performed for 48 h, and the yield is 3.8 times that of an IPTG induction mode.

The present invention belongs to the field of biomass technology and specifically discloses a method for preparing vanillin by catalytic oxidation of vanillyl alcohol. An ionic liquid is used as a reaction solvent. Vanillyl alcohol, acetic acid, a catalyst, and hydrogen peroxide are added to the ionic liquid and heated and stirred under certain temperature and time conditions. After the reaction is completed, the mixture is cooled to room temperature, deionized water is added, and a certain amount of ethyl acetate is added to the system for extraction to obtain vanillin. The present invention develops an ionic liquid reaction system that influences the catalytic performance of the catalyst by regulating the structure of the ionic liquid, inhibits peroxidation side reactions, and improves the selectivity of the vanillin product. Furthermore, the present invention can prepare vanillin by catalytic oxidation of vanillyl alcohol in a single step at a relatively low temperature and under normal pressure air conditions. This process has mild operating conditions, high catalytic activity, and is environmentally friendly, conforming to the concept of green process engineering.