55 results about "Host bacterium" patented technology

The invention provides an anti-white spot syndrome virus peptide derived from litopenaeus vannamei, and belongs to the technical field of biology. The amino acid sequence of the anti-white spot syndrome virus peptide provided by the invention is as shown in SEQ ID NO.1, and the nucleotide sequence of the coding gene is as shown in SEQ ID NO.2. The invention further provides a recombinant expression vector and host bacteria containing the anti-white spot syndrome virus peptide gene, and application of the recombinant expression vector and host bacteria in anti-white spot syndrome virus drugs, vaccines, feeds and feed additives, and the recombinant expression vector and host bacteria have wide application prospects and economic benefits.

The invention provides a method for improving the expression level of a meat toxin light chain. The method comprises the following steps: connecting a dissolution-promoting tag to an N end or a C end of meat toxin light chain protein to form soluble meat toxin light chain recombinant protein; the dissolution-promoting tag is a wild dissolution-promoting tag NT11 or a mutant protein mut7 of the wild dissolution-promoting tag NT11, enzyme digestion removal is not needed, the activity of a meat toxin light chain is hardly influenced and interfered, and the fusion protein can keep 93% of substrate enzyme digestion activity. The method further comprises overexpressing a molecular chaperone htpG in the genome of the host bacterium. According to the invention, a BoNT / A-LC high-efficiency expression system is constructed through multi-strategy optimization, so that the yield of BoNT / A-LC is increased by nearly 20% compared with that of wild type NT11-Lc, the yield reaches 647 mg / L through overexpression of a molecular chaperone htpG, and after combinatorial optimization, the yield is 8.5 times that of only expressed BoNT / A Lc, 1.79 times that of NT11-Lc and 1.43 times that of Mut7-Lc.

The application discloses a kind of exogenous bacteriophage inoculation methods for strengthening soil microbial arsenic methylation capacity, comprising the following steps: S1. preparation bacteriophage crude extract;S2. preparation concentrated solution;S3. preparation bacteriophage extract;S4. preparation inoculation soil sample;S5. in the 2, 4, 6th day of culture, bacteriophage extract is added to inoculation soil;S6. after three times inoculation, continue to flood culture 9 days.The application proposes a kind of inoculation method for retaining virulent bacteriophage, not retaining original host bacteria, inoculating after flooding two days, and inoculating three times, so that the proportion of lysogenization of bacteriophage in soil after inoculation is increased by 156%~347%, so that arsenic metabolism genes carried by bacteriophage genome can function, the arsenic methylation capacity of soil microorganism is strengthened, and the arsenic methylation capacity is increased by more than 300%, to efficiently methylate arsenic in soil.

The application discloses a biosynthesis method of blue copper peptide, comprising the following steps: synthesizing a recombinant protein according to an amino acid sequence shown in SEQ ID NO. 1, synthesizing a gene coding the recombinant protein, constructing a recombinant plasmid by using the gene, transferring the recombinant plasmid into a host bacterium, carrying out fermentation culture to induce protein expression, separating the protein, carrying out enzyme cutting and purification, and finally carrying out complexing with copper ions to obtain the blue copper peptide. Compared with the prior art, the biosynthesis method of the blue copper peptide has the advantages of low production cost, green environmental protection and high yield by optimizing the design of the fusion protein, and the yield of the final product can be significantly improved, and the fusion protein has better structure and isoelectric point characteristics.

This invention discloses a method for the efficient synthesis of gastrodin using recombinant Corynebacterium glutamicum, belonging to the fields of genetic engineering, fermentation engineering, and synthetic biology. This invention uses Corynebacterium glutamicum as the host bacterium and knocks out… pobA The gene was expressed, and the engineered strain WN01 / pB6 was constructed using the expression plasmid pEC-XK99E to express cladoid-pyruvate lyase, carboxylic acid reductase, and glycosyltransferase, achieving heterologous synthesis of gastrodin. Further overexpression was then performed. aroG Genes, overexpression of protein-coding genes that activate carboxylic acid reductase sfp The engineered strain WN05 / pB6 was able to synthesize 7.37 g / L of gastrodin at the shake flask level, and the gastrodin yield reached 17.77 g / L at 72 h of batch fermentation in a 5-L fermenter, with a yield of 0.247 g / L / h. This provides a brand-new biomanufacturing platform for the industrial production of gastrodin.

This invention discloses an anti-phage system based on ESCRT-III and its applications, relating to the field of biotechnology. This invention utilizes the coding genes for two structural proteins of the Hoda_Snf7_1 and Hoda_Snf7_2 from the Hoda archaea ESCRT-III. Hoda_ snf7_1 and Hoda_snf7_2 This invention is applied to antiphage systems. Compared with existing technologies, the main advantages of the antiphage system of this invention are: (1) it consists of only two genes, making system construction simple and quick; (2) it is composed of structural proteins, making it less prone to losing antiphage activity due to gene mutations; (3) it has a broad-spectrum antiphage effect; and (4) it does not affect the normal growth of host bacteria. This system expands the understanding of antiphage systems and provides new ideas and technical means for preventing and controlling phage contamination in industrial microbial production processes.

The invention discloses a recombinant prokaryotic expression vector, an expression strain, soluble human-derived protein and application, the recombinant prokaryotic expression vector comprises a first nucleotide sequence for coding a target human-derived protein and a second nucleotide sequence for coding a cold-adapted molecular chaperone, and a rare codon corresponding to host bacteria is introduced into the first nucleotide sequence to obtain a recombinant prokaryotic expression vector. The translation rate of a key site is actively reduced, a cold-adapted molecular chaperone is introduced into a second nucleic acid sequence, and correct folding and soluble expression of the human-derived protein in a prokaryotic host are promoted in a low-temperature induction manner, so that the high-yield and high-solubility human-derived soluble protein is obtained. The invention provides a feasible way for large-scale preparation of human soluble protein, and has important industrial application value.

The present invention relates to a method for constructing a host bacterium with high expression of human serum albumin, which method comprises: S100, designing an optimized gene encoding human serum albumin; S200, constructing a recombinant expression vector; and S300, transforming the recombinant expression vector into a Pichia pastoris host bacterium. The optimization design of a full-length sequence of a target gene is performed at the molecular level to obtain a target gene with the most suitable expression in Pichia pastoris; at the cellular level, the latest international Pichia pastoris expression system host bacterium CBS7435 is introduced for the gene expression of human serum albumin; and Pichia pastoris transformants harboring high-copy-number exogenous genes are selected by means of using a G418 resistance gene for the construction of an engineering bacterium with high expression of recombinant human serum albumin.

This invention relates to the fields of environmental monitoring and biosafety technology, and in particular to a viral aerosol sampling patch for face masks, comprising: a capture layer for intercepting viral aerosol particles in the air; a culture layer disposed inside the capture layer, the culture layer comprising a culture unit supporting the virus to maintain activity and replicate in the presence of a host, a host bacterium sensitive to the virus, and a visual indicator indicating viral replication or host response; and a base layer disposed inside the culture layer; the sampling patch is fixed to the face mask through the base layer, and viral aerosol collection is achieved without external power through the capture layer, and countable detection results are generated after sampling through the culture layer; utilizing the airflow and humidity conditions naturally formed during wearing, effective interception, activity preservation, and visual detection of viral aerosols are achieved without complex equipment and additional operating steps, and the operation is simple and repeatable.

This invention belongs to the field of protein engineering and biomedicine, and particularly relates to a water-soluble membrane protein, a recombinant vector, a recombinant host bacterium, and their modification methods and applications. The method involves the following steps: First, an interface mutant is constructed based on the SQTY code, and its water solubility and ligand binding ability are evaluated. If the requirements are not met, multiple low-impact transmembrane regions are screened, and after mutation modification, the interface mutant is introduced to construct a single-transmembrane combined mutant, whose water solubility and ligand binding ability are evaluated. If the requirements are still not met, the multiple low-impact transmembrane regions are combined in pairs, and the interface mutant is introduced to construct various double-transmembrane combined mutants, whose water solubility and ligand binding ability are evaluated, and the optimal double-transmembrane combined mutant is selected. This method rationally mutates CXCR4 in stages to achieve water solubility, minimizing changes to the protein's structure and other physicochemical properties, thereby maintaining or even enhancing its binding ability to the ligand CXCL12.

The invention relates to a methanophilic genetically engineered bacterium for producing gamma-polyglutamic acid (gamma-PGA) as well as a construction method and application of the methanophilic genetically engineered bacterium, EQU2420670 gene is knocked out on a host bacterium genome, and meanwhile, an exogenous gamma-PGA synthetase encoding gene cluster pgsBCAE under the control of an inducible arabinose promoter (Pbad) is introduced at the site. The construction method comprises the following steps: obtaining a multi-cistron structure DNA fragment pgsBCAE for coding gamma-PGA synthetase through artificial gene synthesis in combination with a codon optimization strategy, and constructing the expression module Pbad-pgsBCAE under the control of a Pbad promoter. A suicide vector pK18mobsacB carrying a SacB sucrose reverse selection system is used for constructing a gene replacement vector, upstream and downstream homologous arms of an EQU2420670 gene on a genome of methanophilic bacteria M. buryatase 5GB1 are designed on two sides, an EQU2420670 site is replaced by a Pbad-pgsBCAE expression module, the methanophilic genetically engineered bacterium PGA is obtained, and the methanophilic genetically engineered bacterium PGA is applied to production of gamma-PGA and has important economic and social significance; the gamma-PGA produced by the engineering methanophilic bacteria by using methane as a carbon source and an energy source can be used as a cosmetic additive; the invention provides a novel low-carbon biosynthesis approach for commercial production and application of gamma-PGA, and has a good industrial application prospect.

The application discloses a method for removing chaperone and application thereof. The method for removing chaperone comprises the following steps: after a sample containing a target recombinant protein is loaded on an affinity chromatography column, a washing liquid containing urea and KCl is used to flush the affinity chromatography column, so as to remove the chaperone combined with the target recombinant protein. The method of the application can effectively remove the chaperone of about 60 kDa from an escherichia coli host bacterium by combined washing of urea and KCl, and has the advantages of simple operation, low cost, good chaperone removal effect for various recombinant proteins with different molecular weights, wide universality, and the like, and can significantly improve the purity and quality of the recombinant protein.

Disclosed herein are methods and compositions for increasing culture stability of a bacterial culture comprising a plurality of a modified bacterium comprising a modified prophage. Also disclosed herein are methods and compositions for decreasing the likelihood of replication of a modified prophage in a bacterial genome as compared to the likelihood of replication of a corresponding prophage lacking the prophage modification in a corresponding bacterial genome. Also disclosed herein are methods and compositions for preventing excision of a prophage in a bacterium. Also disclosed herein are methods and compositions for generating a modified prophage having an impaired ability to lyse a host bacterial cell.

DEPCT6604 / 07 / 2566 This invention involves a DNA structure suitable for expression. Recombinant proteins in bacterial host cells that include the following DNA structure: Coding nucleotide sequence: promoter rhaBAD, RhaR transcription activater, RhaS Transcription activators, antibiotic resistance indicators, actively linked promoters. Along with nucleic acids that encode indicators of antibiotic resistance, the terminators rrnBT1 and rrnBT2. The point for initiating self-replication, pMB1, is a DNA structure that may also be assembled with sequences. The nucleotides that encode the recombinant protein are functionally linked to the promoter. This invention, rhaBAD, involves, in addition to a vector and a bacterial host cell that are combined. Given this DNA structure and the method of producing recombinant proteins... By contacting the host bacterial cells with rhamnose, the expression of these cells is stimulated. Of the aforementioned recombinant proteins;

One aspect of the invention provides a method of generating bacteriophages adapted to infect a target bacterial strain. The method comprises: providing host bacteria that are susceptible to phage as input to a host chemostat containing phage; providing target bacteria that are related to the host bacteria, but not susceptible to phage as input to a target chemostat containing phage; filtering outflows from the host chemostat and the target chemostat to isolate phage from the populations of the host bacteria, the target bacteria, and macromolecules; combining the outflows; and introducing the combined outflow into each of the host chemostat and the target chemostat.

The invention relates to a phage cocktail and application thereof, and belongs to the technical field of biology. The phage cocktail disclosed by the invention can be used for carrying out fluorescence labeling imaging and efficient killing on bacteria and infectious bacteria in a biofilm of the bacteria. A luminous photosensitizer, glucose oxidase and horse radish peroxidase are coupled to the surface of the phage to obtain three phage conjugates with different functions, and the three phage conjugates jointly form the phage cocktail. The three bacteriophage conjugates are simultaneously colonized in host bacteria by utilizing the targeting ability of the bacteriophages, and show efficient targeting, and the killing efficiency on the host bacteria can reach 100%. The phage cocktail can specifically recognize and fluorescently label bacteria in a biofilm, can efficiently destroy the structure of the biofilm and thoroughly eradicates the biofilm and antibiotic-sensitive and drug-resistant host bacteria in the biofilm.

The invention provides enterococcus faecalis bacteriophage vBEfaS-1017 and application thereof, the enterococcus faecalis bacteriophage vBEfaS-1017 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.46182, and the preservation time is September 6, 2024. The bacteriophage vBEfaS-1017 is high in specificity, short in incubation period and ultrahigh in stability, and has activity under the conditions of extreme pH, high temperature and ultraviolet radiation; by evaluating the in-vitro bactericidal efficacy of the bacteriophage vBEfaS-1017, it is determined that the bacteriophage vBEfaS-1017 can effectively remove a biological membrane formed by host bacteria Ef 10-17, the removal rate is as high as 90%, and the bacteriophage vBEfaS-1017 is expected to become a scavenger for solving the biological membrane. Further experiments prove that the bacteriophage vBEfaS-1017 has in-vivo and in-vitro safety. Through combined use of the bacteriophage and different antibiotics, it is found that combined use of the bacteriophage vBEfaS-1017 and the antibiotic levofloxacin can effectively treat bacteremia of mice, the treatment time after infection can be prolonged by using the bacteriophage in advance, and the bacteriophage has a good application prospect in the aspect of preventing and treating enterococcus faecalis.

The invention relates to application of an ACADM gene in increasing the yield of spinosad in saccharopolyspora spinosa, and belongs to the field of biological fermentation. The method mainly comprises the step of overexpressing the ACADM gene in the saccharopolyspora spinosa. Specifically, the ACADM gene is introduced into the saccharopolyspora spinosa, and the ACADM gene is efficiently expressed in the host bacterium, so that the biosynthetic pathway of the spinosad can be effectively promoted, and the final yield of the spinosad is remarkably increased. Experiments show that the method is feasible to operate and remarkable in effect, and an effective genetic modification strategy is provided for industrial production of spinosad.

This invention discloses a laccase derived from *Coprinus gracilis*, its recombinant expression strain, and a highly efficient expression method, belonging to the fields of microbiology and bioengineering technology. Using *Aspergillus niger* MA70.15 as the experimental strain, this invention employs a random integration strategy to achieve pC3-P... citA -SP GlaA - lcc9‑ T trpC ‑pyrG The expression vector was integrated into the upstream region of the promoter of the host bacterium citrate synthase in the form of nine consecutive copies. To investigate the effects of microparticles and osmotic regulators in the fermentation medium on laccase production by recombinant Aspergillus niger, this invention added different types and concentrations of microparticles and osmotic regulators, resulting in a laccase activity of 1566.7 U / L in shake-flask fermentation of recombinant strain AnRcgL1 and 1961 U / L in a 1 L fermenter, successfully achieving efficient recombinant expression of laccase Lcc9 in Aspergillus niger.

The invention discloses a pseudomonas putida KT2440 endogenous PUTR library, a construction method and engineering bacteria for efficiently synthesizing rhamnolipid. KT2440 is used as host bacteria, key genes are synthesized by heterologous expression of rhamnolipid, recombinant plasmids are constructed, and the host bacteria are introduced to obtain engineering strains; carrying out fermentation culture on the wild type and the engineering strain under the same condition, and carrying out comparative transcriptomics analysis on samples in different growth stages; on the basis of transcriptome data and the key gene, acquiring a PUTR candidate element at the upstream of a starting site of a key gene sequence, connecting the PUTR candidate element to a carrier genome containing fluorescent protein, and introducing into a host bacterium competent cell to obtain a genetic engineering strain; and screening different PUTR elements to optimize key genes in a rhamnolipid synthesis route to obtain the engineering bacteria. The carbon flow distribution is accurately regulated and controlled, the synthesis efficiency of the rhamnolipid is remarkably improved, and the engineering applicability and practical value of the rhamnolipid in synthesis of the high-added-value biosurfactant are verified.

This invention belongs to the field of bioengineering technology, specifically relating to a gene for efficiently expressing thymosin α1 (Tα1), recombinant lactic acid bacteria, and their applications. This invention provides a Tα1 encoding gene specifically optimized for *Lactococcus lactis* NZ9000, which significantly improves expression efficiency in the host through full-sequence codon adaptation. This gene was cloned into the vector pNZ8148-M, constructing the recombinant expression plasmid NZ9000 / pNZ8148-M. usp45 + T α1 The strain was successfully transformed into the host bacterium NZ9000, yielding recombinant lactic acid bacteria NZ9000 / pNZ8148-M. usp45 + Tα1 This recombinant strain can efficiently secrete the Tα1 fusion protein USP under Nisin induction. 45 +Tα1, the protein is digested in vitro by enterokinase to release active Tα1. The Tα1 produced by the method of this invention has high yield, high purity, high biological activity and excellent safety, providing a new technical platform for the low-cost, large-scale and safe production of Tα1.

Provided is a method for infecting a bacterium, in which a host bacterium is exposed to a phage depolymerase and a bacteriophage. The depolymerase catalyzes degradation of at least one cell-surface or extracellular polysaccharide of the host bacterium, thereby increasing the susceptibility of the host bacterium to infection by the bacteriophage. The method may be used to increase production of the bacteriophage and / or to increase the host range of the bacteriophage. Further provided is use of a phage depolymerase selected from B5, As11, TaPaz, APK2, APK37, and IME200, or a composition comprising the same, for increasing the susceptibility of an A. baumannii to infection by a bacteriophage. The A. baumannii may be an AB030, MRSN 843, MRSN 1183, MRSN 7446, MRSN 15088, MRSN 24008, MRSN 31159, MRSN 31468, or MRSN 32866 strain.

This invention relates to an expression vector, engineered bacteria, and method for inducing expression of a serine protease. The expression vector contains a nucleic acid sequence encoding a serine protease, and the amino acid sequence of the serine protease includes the sequence shown in SEQ ID NO.1. This invention transforms the expression vector into a host bacterium and utilizes its unique high-efficiency expression system for protein expression. By optimizing culture and induction conditions, high-efficiency expression of the serine protease was successfully induced. This serine protease exhibits highly efficient hydrolytic ability, with an enzyme activity reaching 925 U / mL.

The invention belongs to the technical field of bioengineering, and relates to a trichoderma reesei engineering bacterium with high xylanase yield, a host bacterium and application of the trichoderma reesei engineering bacterium. The engineering bacterium consists of a host bacterium and an expression cassette, wherein the expression cassette comprises a Ptcu promoter, a signal peptide of CBH II, a CBM structural domain and a xylanase XynST11 optimized sequence; the host bacterium is a Trichoderma reesei auxotroph strain QM9414-delta pyr4, and the Trichoderma reesei auxotroph strain QM9414 The invention also provides a host bacterium. The host bacterium is a strain obtained by knocking down a Vps10 coding gene of a trichoderma reesei auxotroph strain QM9414-delta pyr4 or overexpressing rho3 at a vps10 site. According to the trichoderma reesei engineering bacteria with high xylanase yield, the expression and secretion of the xylanase XynST11 in the trichoderma reesei are promoted by using the signal peptide of the CBHII, and the secretion of protein can be more effectively promoted by further fusion with the CBM structural domain of the xylanase XynST11.

The invention discloses a bordetella bacteriophage with good acid tolerance as well as a composition, a kit and application of the bordetella bacteriophage, and belongs to the technical field of bacteriophages. The bacteriophage is bordetella bacteriophage BOP2ABONT11, the preservation number of the bacteriophage BOP2ABONT11 is GDMCC NO.67044-B1, and the bacteriophage BOP2ABONT11 has a nucleotide sequence as shown in SEQ ID No.1. The bacteriophage has a polyhedral stereosymmetric head and a short contracted tail, is strong in lysis capability, can identify 92% of bordetella, and has titer of 4.2 * 10 PFU / mL after being cultured for 8 hours under the condition that MOI is 0.001; the acid resistance and heat resistance are excellent, and the catalyst still has activity after being treated for 96 hours at pH 3 and is stable at 55-65 DEG C; the strain does not contain virulence genes, is high in safety and has no splitting action on non-host bacteria. The invention also provides a composition containing the bacteriophage, and a biological bactericide, a medicine, a feed additive and a kit prepared from the bacteriophage or the composition. The bacteriophage and related products can efficiently prevent and treat Bordetella infection, are applied to the fields of environment disinfection, food preservation, animal breeding, pathogenic bacterium detection and the like, and have wide application prospects.

The application discloses a polypeptide for presenting a heterologous protein to an outer membrane vesicle and application thereof. Specifically disclosed are a polypeptide with an amino acid sequence of SEQ ID No. 1 and application thereof in presenting a heterologous protein to an outer membrane vesicle of a host bacterium. The application successfully expresses the heterologous protein in OMVs of Xanthomonas campestris by connecting the polypeptide with the heterologous protein. Xanthomonas campestris is a plant pathogenic bacterium, has low immunogenicity to human bodies, has a unique advantage for biomedical application of OMVs, and realizes presentation of the heterologous protein in Xanthomonas campestris OMVs for the first time, thereby providing a solution for future plant biotechnology, development and application of a vaccine adjuvant and the like. The polypeptide of the application realizes presentation of the heterologous protein by using only 25 amino acids, and can minimally affect the conformation and activity of the presented protein.

The invention provides recombinant escherichia coli for synthesizing vanillyl alcohol as well as a construction method and application of the recombinant escherichia coli. Wherein the recombinant escherichia coli comprises a host bacterium, and the host bacterium overexpresses a DAHP synthetase encoding gene AroGfbr and a 3-dehydroshikimic acid dehydratase encoding gene QuiC on an escherichia coli genome by using a pTrc promoter, so that sufficient supply of protocatechuic acid is realized; an alcohol dehydrogenase encoding gene ADH4 or ADH6 is introduced, so that the conversion speed of a toxic intermediate 3, 4-dihydroxybenzaldehyde is accelerated, and the fermentation density of the strain is improved; genes mtn and luxs of a methylation cofactor SAM regeneration pathway are over-expressed on a genome, so that the methylation efficiency is improved. Therefore, the recombinant Escherichia coli provided by the invention can efficiently synthesize vanillyl alcohol through the combination of the transformation strategies, and can efficiently accumulate 1.66 g / L vanillyl alcohol in a shake flask, and the yield of a 3L fermentation tank reaches 6.21 g / L. The method for producing vanillyl alcohol by using the recombinant strain provided by the invention has the advantages of low cost, controllable process, simplicity and convenience in separation and extraction, and facilitation of industrial production.

This invention discloses an engineered methanogenic bacterium that produces high levels of squalene, its construction method, and its applications, belonging to the fields of metabolic engineering and synthetic biology. This is achieved by using a strong promoter in the host bacterium (methanogenic bacterium). P mxaF Expression of squalene synthase encoding gene sqs Overexpression of the gene encoding 1-deoxy-xylulose-5-phosphate synthase in the squalene synthesis pathway dxs1 and dxs2 Farnesyl pyrophosphate synthase encoding gene ispA Isopentenyl pyrophosphate isomerase encoding gene My friends Simultaneously overexpressing glucose-6-phosphate dehydrogenase zwf1 and the EMP pathway pfk1 and fba2 An engineered methanogenic bacterium producing squalene was obtained. Using this engineered methanogenic bacterium for squalene fermentation production can significantly increase squalene yield and has excellent prospects for industrial application.

The invention discloses a giant strong aeromonas hydrophila bacteriophage and application thereof, and belongs to the technical field of microorganisms. According to the invention, aeromonas hydrophila is taken as host bacteria, the phage is enriched from a collected lake water sample, and is separated and purified by adopting a double-layer agar plate method, so that a strain of phage is successfully obtained, the strain of phage is named as aeromonas hydrophila phage Cgtyf, the preservation number is CCTCC NO: M 2026099, and the titer of the phage can reach 1.0 * 10 < 10 > PFU / mL; the genome size of the phage is about 237 kb, and the phage belongs to a giant phage. In an in-vitro bacteriostasis experiment, the bacteriophage Cgtyf shows a remarkable bactericidal effect and a relatively strong growth inhibition capability on host bacteria, and shows a relatively high temperature tolerance range in a temperature stability test. Therefore, the bacteriophage Cgtyf provided by the invention can be effectively used for preventing and controlling aeromonas hydrophila in aquatic products, has a potential application value as an antibacterial drug substitute, is beneficial to reducing or avoiding the use of antibacterial drugs in aquaculture, and has a good application prospect.

The invention discloses a GutAcraca family protein as well as a screening method and application thereof in gene editing, and belongs to the technical field of microbial immune regulation and control. The method comprises the following steps: extracting a CRISPR spacer sequence from an intestinal bacterial genome, comparing the CRISPR spacer sequence with bacteriophage genome data, and identifying a bacteriophage which has a potential interaction relationship with host bacteria carrying a type II CRISPR system and can encode an Aca homologous protein containing an HHT structural domain; and then predicting candidate Acr genes in an adjacent region of the Aca homologous gene, cloning, transforming, carrying out multi-round passage enrichment survival cloning, carrying out deep sequencing and relative abundance dynamic analysis on an enriched population, and screening out functional Acr proteins of which the relative abundance slope is greater than zero in a passage process, so as to obtain the GutAcraca family proteins. The protein has structural astringency, has a highly similar three-dimensional structure although the sequence is highly heterogeneous, has TM-score greater than or equal to 0.5, generally contains a conservative HTH motif, has double functions of a CRISPR-Cas inhibitor and transcription self-inhibition, and can be used for regulating and controlling a CRISRP system and gene editing.