13 results about "Extracellular protease" patented technology

The application discloses a preparation method of a novel inhalable anthrax component vaccine based on a mutant strain of anthrax bacillus, and relates to the technical field of immunology medicine. The application provides a mutant strain of anthrax bacillus, and a mutant strain A16R-5.1 with six extracellular protease activity related genes deleted. The vaccine is extracted from the culture supernatant of anthrax prepared by using the mutant strain A16R-5.1, and can induce strong humoral, cellular and mucosal immune responses after immunization, can resist the invasion of anthrax spores, and can neutralize anthrax toxin in an in-vitro experiment.

The invention discloses a quick vinasse fish production method for promoting co-fermentation of fish and rice, belongs to the technical field of aquatic product processing, and aims at the technical bottlenecks of unbalanced fermentation power, high acidification risk, relatively hard texture and long fermentation period of traditional vinasse fish. The method comprises the core steps of raw material pretreatment, low-temperature pickling, temperature-controlled drying, glutinous rice grading and curing, nutrient enrichment distiller's yeast mixing, composite functional steeping liquor preparation, fish block steeping, layered canning and fermentation. The layered design of the fully-cooked glutinous rice and the half-cooked glutinous rice ensures continuous and stable supply of a carbon source and avoids fermentation imbalance, and meanwhile, a nutrient enrichment system optimizes a fermentation microenvironment and an enzymology basis; the glutinous rice nutrition system promotes microorganisms to secrete extracellular protease, the composite functional steeping liquor activates endogenous protease of the fish meat, and the two components cooperate to efficiently soften the fish meat texture. The final product retains the traditional vinasse fish core flavor, the fermentation period is remarkably shortened, the texture is soft and tender, the flavor is coordinated, the quality stability is outstanding, and the industrial application prospect is good.

This invention discloses a protease-responsive GPCR receptor and its applications. The protease-responsive GPCR receptor of this invention comprises an extracellular protease recognition domain, a transmembrane domain, and an intracellular signaling domain; the extracellular protease recognition domain contains a peptide hydrolyzed by the protease; the intracellular signaling domain contains an HCV enzyme hydrolyzed peptide, a nuclear localization sequence (NLS), a Cas protease, and a coupled nuclear transcription factor. This protease-responsive GPCR receptor can induce endogenous dCas9 assembly into the nucleus and activate the chimeric antigen receptor signaling pathway in natural killer cells in response to extracellular proteases, providing a new solution for developing novel precision immunotherapy.

The invention relates to the field of insect prevention and control, in particular to a high-stress-resistance beauveria bassiana strain CIPPBb075 with high pathogenicity to grassland caterpillars and application of the high-stress-resistance beauveria bassiana strain CIPPBb075. According to the invention, diseased insects parasitized by beauveria bassiana are collected from a field, and the strain is separated and purified, so that the CIPPBb075 strain has relatively high toxicity to grassland caterpillar; the germination rate is increased along with temperature rise, and the spore germination rate is obviously higher than that of other strains; the ultraviolet resistance at 25 DEG C is high; the extracellular protease activity is high; the activity of extracellular chitinase is high. Therefore, the CIPPBb075 strain has relatively strong stress resistance, and can be used as an effective microbial agent for preventing and treating the larvae of the grassland caterpillar compared with other strains.

The present application relates to the field of insect control, in particular to a strong stress-resistant Beauveria bassiana strain CIPPBb075 with high pathogenicity to lema frisoni and its application. The present application is collected from the field by the diseased insects parasitized by the Beauveria bassiana, and the strain is separated and purified, the CIPPBb075 strain has high virulence to lema frisoni; the germination rate increases with the increase of temperature, and the spore germination rate is significantly higher than that of other strains; the ultraviolet resistance is high at 25 DEG C; the extracellular protease activity is high; the extracellular chitinase activity is high. Therefore, the CIPPBb075 strain has strong stress resistance and other strains, and can be used as an effective microbial source drug for controlling lema frisoni larvae.

The invention provides a method for preparing skin-brightening cubilose fermentation filtrate based on a precise fermentation technology and application of the skin-brightening cubilose fermentation filtrate. Dried cubilose and a carbon source solution are sterilized, stood, cooled and mixed into a fermentation culture medium, and then activated bacillus fungicide is inoculated into the fermentation culture medium to obtain a cubilose fermentation culture medium. According to the application, microorganisms are utilized to ferment and metabolize the cubilose, extracellular protease and the like, so that the composition of cubilose substances is changed, the content of functional components in fermentation filtrate is increased, and release of skin color brightening related functional activity is accelerated and optimized. The cubilose filtrate fermented by the specific bacillus obviously reduces the melanin content and the heme content of the skin, improves the brightness of the skin, reduces the redness and the yellowness of the skin, and has the effects of removing black, removing red, removing yellowness, extracting the skin and the like; compared with enzymolysis cubilose peptide fermentation filtrate in the prior art, the accurate cubilose fermentation filtrate is free of bitter taste and peptide taste through sensory evaluation, and a solid technical foundation is laid for developing efficient and easily-absorbed cubilose-source skin-brightening functional products.

The invention relates to a method for preparing a bacterial supernatant. The method comprises the following steps: culturing Pseudomonas environmental strain PF-11 cells; and recovering the supernatant. The invention also relates to a method of reducing the amount of biofilm on a surface, reducing adhesion of at least one organism on a surface, reducing small and large area contamination on a surface, comprising contacting the surface with a supernatant, a supernatant fraction, a modified supernatant or a modified supernatant fraction of Pseudomonas strain PF-11; or a composition comprising a supernatant, a supernatant fraction, an improved supernatant or an improved supernatant fraction of the Pseudomonas strain PF-11, and one or more acceptable carriers. The invention also relates to a method of killing or reducing the growth of fungi or bacterial cells, or killing or inhibiting the development of insects or marine copepods, comprising contacting the fungi, bacteria, insects or marine copepods with a supernatant, a supernatant fraction, an improved supernatant or an improved supernatant fraction of a Pseudomonas strain PF-11 culture; or a composition comprising a supernatant, a supernatant fraction, an improved supernatant or an improved supernatant fraction of a Pseudomonas strain PF-11 culture, and one or more acceptable carriers. The invention also relates to a substantially pure culture of the Pseudomonas strain PF-11. The invention also relates to a culture enriched in the Pseudomonas strain PF-11. The invention also relates to a method of identifying whether a bacterium is capable of producing one or more extracellular proteases capable of digesting high molecular weight substrates.

The invention discloses a strain for efficiently synthesizing gamma-glutamyl peptide and application of the strain, and is characterized in that the strain is rhodotorula mucilaginosa EIODSF019, is preserved in the China General Microbiological Culture Collection Center (CGMCC), has the preservation number of CGMCC No. 32529, and has the preservation number of CGMCC No. 32529. The invention further provides application of the rhodotorula mucilaginosa EIODSF019 in production of extracellular protease, production of gamma-glutamyl transpeptidase and synthesis of gamma-glutamyl peptide, and application of the rhodotorula mucilaginosa EIODSF019 in fermentation of dry-cured hams. The compound enzyme has the advantages of high protease activity and high gamma-glutamyltranspeptidase activity, and can significantly improve delicate flavor and strong flavor when being applied to fermented meat products.

The invention discloses streptomyces LZZY-S40 with broad-spectrum bacteriostatic activity and growth promoting effect and application thereof, and belongs to the technical field of microorganisms. The invention provides streptomyces sp. LZZY-S40 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.30320 in order to solve the problems that in the prior art, streptomyces mostly focuses on single function research, the antibacterial spectrum is relatively narrow, and the agricultural production requirement that various diseases coexist is difficult to meet. The bacterial strain has good broad-spectrum bacteriostatic activity on plant pathogenic fungi, and the bacteriostasis rate of the bacterial strain on plant pathogenic bacteria xanthomonas semitransparent reaches 100%; a spore suspension of the strain has a remarkable promoting effect on wheat root length, bud length and wheat fresh weight; in addition, the streptomyces LZZY-S40 strain can also be applied to preparation of microbial agents for producing extracellular protease, auxin, siderophore, nitrogenase and ACC deaminase.

The invention discloses application of tRNA modified enzyme MiaA in prevention and control of pseudomonas aeruginosa, and belongs to the technical field of prevention and control of pathogenic bacteria. According to the invention, it is found for the first time that tRNA modification enzyme MiaA plays a crucial role in the anti-oxidative stress reaction and pathogenic process of pseudomonas aeruginosa, and the nucleotide sequence of the coding gene MiaA is shown as SEQ ID NO. 1. The pseudomonas aeruginosa miaA deletion mutant is constructed through a gene knockout method, and experimental results show that resistance of the miaA deletion mutant to acidity, osmotic pressure and oxidation pressure is remarkably reduced, and the miaA deletion mutant is highly sensitive to hydrogen peroxide; meanwhile, the pathogenicity of the miaA deletion mutant is remarkably reduced, and the yield of a key pathogenic factor biological membrane, the activity of extracellular protease and the swimming ability are remarkably reduced. The invention provides a new gene target for prevention and control and drug resistance treatment of pseudomonas aeruginosa, and has outstanding clinical application value.

Provided are a recombinant Bacillus subtilis, and a construction method therefor and a use thereof. By means of CRISPR / cas9 technology, a starting strain B.S168Δ2 with a sporulation membrane serine protease htrC and an alkaline serine protease aprX knocked out is constructed first; then on the basis of the starting strain B.S168Δ2, eight chassis cells with extracellular protease genes knocked out are constructed, namely, B.S168Δ3, B.S168Δ4, B.S168Δ5, B.S168Δ6, B.S168Δ7, B.S168Δ8, B.S168Δ9 and B.S168Δ10; chassis cells B.S168Δ8, B.S168Δ9 and B.S168Δ10, which have high transformation efficiency, no residual resistance, and almost no extracellular proteases and do not affect accumulation of extracellular target proteins, are selected; then, on this basis, the chassis strain B.S168Δ9 which is most suitable for secreting and expressing pullulanase is selected. Compared with the original strain B.S168, the enzyme activity of the constructed recombinant strain B.S168Δ9amyX is remarkably improved.

The application discloses a strain for efficiently synthesizing gamma-glutamyl peptides and application thereof, and has the characteristics that the strain is Rhodotorula mucilaginosa EIODSF019, is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No.32529; the application of the Rhodotorula mucilaginosa EIODSF019 in producing extracellular protease, producing gamma-glutamyl transpeptidase and synthesizing gamma-glutamyl peptides, and in fermented dry-cured ham is also provided; and the Rhodotorula mucilaginosa EIODSF019 has high protease activity and high gamma-glutamyl transpeptidase activity, and can significantly improve umami and rich flavor when applied to fermented meat products.

The invention relates to the technical field of microbial genetic engineering and fermentation engineering, and particularly discloses an aspergillus oryzae genetic engineering strain and application thereof, the aspergillus oryzae genetic engineering strain is ZW28-deltasA1 deltasB1 deltasC1 deltasD1, and is a quadruple gene knockout strain constructed by taking aspergillus oryzae ZW28 as an original strain and continuously knocking out protease genes AosedA, AosedB, AosedC and AosedD by using a gene editing technology. According to the quadruple gene knockout strain provided by the invention, the extracellular total protease activity is averagely reduced by 35%, the yields of expressed human lysozyme and calf chymosin respectively reach 65 mg / L and 122.2 mg / L, which are 4.7-5.2 times higher than those of an original strain, and the quadruple gene knockout strain shows strong universality for foreign proteins with different sources and pH characteristics, and provides a dominant platform for efficient production of industrial enzymes.