248 results about "Virulence factor" patented technology

The invention relates to a recombined cattle parainfluenza carrier pcDNA-NM09-VP1 for expressing the protein VP1 of a porcine O type foot-and-mouth disease virus. The recombined cattle parainfluenza carrier is characterized in that an RNA (ribonucleic acid) extracted from a strain BPIV3NM09 is used as a template; a virus total-length gene group is segmentally amplified through RT-PCR (reverse transcription-polymerase chain reaction); the total-length cDNA of cattle parainfluenza is subjected to primary modification, namely AgeI is introduced between P and M, so that insertion and replacement of exogenous antigen gene fragments are facilitated; due to secondary modification, antigen epitope which is inserted into a heterologous virus in a modified manner is the protein VP1 of the O type foot-and-mouth disease virus; the amino acid sequence is SEQIDNO1, and the nucleotide sequence is SEQIDNO2. The foundation is laid for further development of a gene engineering recombined vaccine for preventing and controlling the porcine foot-and-mouth disease and research on the III-type toxicity factor and the molecular pathogenesis of the cattle parainfluenza; the recombined cattle parainfluenza carrier has the replication capacity.