7 results about "Regulatory Pathway" patented technology

The application provides application of a TT2-SCT1 / SCT2-WR2 regulation channel in plant heat stress tolerance. The application first discloses a new signal channel involved in plant heat tolerance: a TT2-SCT1 / SCT2-WR2 signal channel; wherein, TT2 is a negative regulation factor for regulating the heat tolerance of plants or protecting (maintaining) the yield of plants in Gramineae under high temperature, SCT1 / SCT2 are also negative regulation factors for regulating the heat tolerance of plants or protecting (maintaining) the yield of plants in Gramineae under high temperature, and WR2 is a positive regulation factor for playing a role, which is beneficial to maintaining the homeostasis in plants. The application has important theoretical significance for genetic improvement of plant traits.

This application relates to the field of tumor metabolic biomarker detection technology, and discloses a method for detecting glucose metabolism biomarkers in esophageal cancer based on VAV2-related molecular characteristics. The method includes: performing immunohistochemical staining on esophageal cancer tissue samples to obtain VAV2, EIF3F, MTA1, and HIF-1α protein expression sections; scoring the immunoreaction of each section; calculating a comprehensive glucose metabolism phenotype score based on the molecular hierarchy of the VAV2-EIF3F-MTA1 / HIF-1α glucose metabolism regulatory pathway; and classifying the samples into high-glycolytic, moderate-glycolytic, and low-glycolytic types according to the comprehensive score. This application improves the accuracy and stability of glucose metabolism phenotype discrimination in esophageal cancer.

The invention belongs to the technical field of plant genetic engineering and molecular breeding, and particularly relates to a new function and application of a transcription factor gene OsMYB87 derived from rice, an OsMYB87 function deletion mutant created through a gene editing technology and application of the OsMYB87 function deletion mutant in increasing the rice grain length and grain weight. The OsMYB87 gene found in the invention shows a key differentiated characteristic: preliminary phenotypic observation shows that the phenomenon that chalkiness of endosperm is remarkably aggravated inevitably due to OsMYB73 knockout is not observed while the function deficiency of the OsMYB87 gene brings a remarkable yield increasing effect (grain length and grain weight increase). The OsMYB87 is strongly prompted to be possibly located on a different regulatory pathway, and as a breeding target, the OsMYB87 provides greater possibility and a better potential choice for maintaining the appearance quality of rice commodities while realizing yield increase, so that the OsMYB87 has higher application value.

This invention discloses a method and application for regulating fatty liver syndrome based on osteocalcin-adiponectin receptor, relating to the field of livestock and poultry metabolic disease prevention and control technology. The method includes the following steps: clarifying the clinical symptoms and pathological changes of fatty liver syndrome in laying hens; constructing a complementary validation system for fatty liver syndrome models, including an in vivo FLS model in laying hens and an in vitro LMH model of chicken liver cancer cells; using osteocalcin OCN and the adiponectin receptor agonist AdipoRon to target and intervene in the in vivo FLS model and the in vitro LMH model of chicken liver cancer cells, and conducting multi-dimensional detection and evaluation of the regulatory effect; by specifically silencing the expression of AdipoR1 or AdipoR2, comparing changes in lipid deposition and signaling pathways in cells treated with osteocalcin, and identifying the functional hierarchy of AdipoR1 and AdipoR2 in the osteocalcin regulatory pathway; this invention achieves precise prevention and treatment of fatty liver syndrome in laying hens through complementary validation models, targeted intervention with osteocalcin and AdipoRon, and identification of adiponectin receptor function.

The invention belongs to the technical field of genes, discloses a method for detecting and mining associated genes and regulatory pathways by using double-layer optimization, solves the black box problem in a machine learning prediction process and provides biological mechanism explanation. The previous research mostly utilizes multi-omics data to predict phenotypes, but a machine learning mode is difficult to really evaluate the action size and action approach of genes, and lacks interpretability at the same time. According to the RADAR, a parameter is given to each omics of each gene for double-layer optimization solution, phenotypes can be explained to the maximum extent, and meanwhile the action degree of each omics can be evaluated. In addition, RADAR can process input data of any number of omics, and a program can automatically identify the number of the omics and calculate the effect size of each omics. RADAR can accept omics input of different gene numbers, and the actual data condition is better met.

The invention discloses a Crassostrea hongkongensis VgR gene and an shRNA-VgR sequence and application thereof, a VgR gene segment is obtained by screening and cloning from a Crassostrea hongkongensis transcriptome, shRNA is designed and synthesized according to the Crassostrea hongkongensis VgR gene sequence, and the shRNA is named as shRNA-VgR. The method comprises the following steps: introducing the VgR gene into Crassostrea hongkongensis by injecting adductor muscle to interfere and inhibit the expression of the VgR gene in the Crassostrea hongkongensis, detecting the expression and location distribution change of the interfered VgR gene, the activity change of related signal channel key genes and key enzymes regulated by the VgR gene, the morphological structure change of related tissues, the cell apoptosis condition and the like, and identifying the Crassostrea hongkongensis in the Crassostrea hongkongensis. And systematically analyzing the effect of the VgR gene and the regulatory pathway thereof in the physiological process of the crassostrea hongkongensis and the regulatory mechanism thereof. The injection dosage is small, the cost can be reduced, and regulation and control of VgR-guided related signal channels on physiological functions of crassostrea hongkongensis can be effectively influenced.

This invention discloses a natural selenium-enriched black soybean sprout endogenous synergistic extract, its preparation method, and its applications, belonging to the fields of food biotechnology and functional foods. Using high-selenium naturally enriched black soybeans as a single raw material, with no exogenous selenium added throughout the process, anthocyanins are enriched through light-protected germination. This is combined with enzymatic hydrolysis and membrane separation purification to construct a multi-component endogenous synergistic system. The extract is rich in highly converted organic selenium, small molecule peptides, anthocyanins, and endogenous phytase, exhibiting characteristics such as resistance to gastric acid, slow release into the intestine, and promotion of mineral absorption. Relying on the synergistic effects of multiple metabolic regulatory pathways, the product can achieve liver protection and detoxification, cell repair, antioxidant effects, and fat reduction. It effectively improves the shortcomings of traditional compound products, such as weak stability, low absorption rate, and large batch-to-batch variations. The production process is stable, with significant industrialization advantages, and it can be widely applied in the fields of functional foods, special dietary foods, and dietary supplements.