129 results about "Network pharmacology" patented technology

The application provides application of grass aconite in preparation of anti-epilepsy drugs and a mechanism analysis method, relates to the technical field of anti-epilepsy drugs, and discloses a "drug active ingredient-key target point-disease-pathway" network diagram of the grass aconite anti-epilepsy by starting from the chemical structure of the effective component of the grass aconite, applying network pharmacology and molecular docking technology, revealing how the active component of the grass aconite plays the drug efficacy through "multi-target point, multi-pathway and multi-pathway" combined regulation, predicting the anti-epilepsy target point and mechanism of the grass aconite, and providing certain theoretical reference for research and development of the grass aconite as an anti-epilepsy drug and a functional food.

The invention relates to the technical field of traditional Chinese medicines, and discloses application of an active ingredient of shiny-leaved yellowhorn leaves in preparation of a medicine for treating hyperuricemia. The xanthoceras sorbifolia leaf active ingredients comprise quercitrin, rutin and myricetin; the content of quercitrin is 0.2508 mg / g to 0.6719 mg / g, the content of rutin is 0.6707 mg / g to 1.5831 mg / g, and the content of myricetin is 0.8002 mg / g to 3.4858 mg / g. According to the invention, myricetin, quercitrin and rutin are determined as core effective substances of shiny-leaved yellowhorn leaves for treating hyperuricemia for the first time; the multi-target and multi-channel action mechanism is disclosed; meanwhile, network pharmacological analysis shows that the three components have high connectivity in a network and jointly act on key targets such as XOD, ACE, AKR1B1 and TNF, it is prompted that the three components do not act singly, but play a treatment role through multi-target and multi-channel cooperation, the traditional'single target-single drug 'thinking is changed into a'multi-component-multi-target-multi-channel' system regulation mode, and the treatment effect is improved. A new thought is provided for comprehensive treatment of the hyperuricemia.

The invention provides a traditional Chinese medicine composition, a traditional Chinese medicine preparation, a preparation method and application, and particularly belongs to the technical field of traditional Chinese medicine. The invention provides a traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following components in parts by weight: 20-50 parts of parched white peony root, 8-15 parts of pawpaw, 10-20 parts of radix clematidis, 20-40 parts of caulis spatholobi, 5-12 parts of honey-fried licorice root, 10-20 parts of fructus broussonetiae, 10-20 parts of radix cyathulae and 5-12 parts of cassia twig. The traditional Chinese medicine composition has great potential in the aspects of treating kOA and inhibiting osteophyte formation, and can be used for improving kOA cartilage injury. Through in-vitro experiments, network pharmacology and in-vivo experiments, the curative effect of XBD in treating kOA and the effect of XBD in inhibiting osteophyte formation are verified, it is proved that XBD has a regulation effect on cartilage degeneration and osteophyte formation, and an important thought is provided for developing innovative drugs with cartilage protection and abnormal osteophyte formation inhibition functions.

The invention discloses a traditional Chinese medicine composition for preventing and treating alcoholic liver injury, and relates to the technical field of traditional Chinese medicines, and the traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 10 to 15 parts of radix puerariae, 6 to 12 parts of semen hoveniae, 6 to 10 parts of radix angelicae sinensis, 6 to 10 parts of fructus lycii and 2 to 5 parts of liquorice root. Based on the treatment rule of'dispelling effects of alcohol, regulating qi and blood and tonifying liver and kidney 'in traditional Chinese medicine, medicinal and edible traditional Chinese medicines such as radix puerariae and hovenia dulcis thunb are primarily screened, and meanwhile, a'multi-target synergistic mechanism' predicted by network pharmacology is constructed and converted into a quantifiable treatment effect. The experimental data not only verifies the key pathway of GO / KEGG enrichment, but also systematically verifies the compound liver protection effect through multi-scale indexes such as visceral organ indexes, serum enzymology, histopathology and the like. The research not only provides a novel compound with a clear mechanism for ALD, but also demonstrates the feasibility of driving the precise design of a traditional Chinese medicine compound by network pharmacology, and lays a methodological foundation for modern development of medicinal and edible resources.

The invention provides a traditional Chinese medicine delivery system of temperature-sensitive gel for allergic rhinitis based on a fructus xanthii formula, belongs to the technical field of pharmaceutical preparations, and solves the technical problems that the existing allergic rhinitis medicines are mainly hormone, have repeated effects and the like. Comprising the following preparation steps: S1, initially preparing a traditional Chinese medicine formula of the cocklebur fruit powder for treating rhinitis; s2, performing raw material level network pharmacology prediction; s3, trial production of a raw material grade temperature-sensitive gel product; s4, carrying out raw material grade experiment testing; s5, carrying out pharmacological prediction on the monomer-level network; s6, carrying out preliminary trial production on the monomer-grade temperature-sensitive gel product; s7, carrying out monomer-level preliminary experiment testing; s8, upgrading the formula; s9, carrying out trial production on the monomer-grade temperature-sensitive gel product again; and S10, carrying out experimental test on the monomer level again to confirm the most effective traditional Chinese medicine formula. According to the invention, a set of closed-loop iteration system of clinical experience-network pharmacology prediction-response surface process optimization-multi-level pharmacodynamic verification is constructed, and a traditional Chinese medicine delivery product which is controllable in quality, clear in mechanism and excellent in curative effect and is used for effectively treating allergic rhinitis can be prepared.

The invention discloses application of jellyfish and snow rabbits in inhibition of ERK phosphorylation level and MAPK pathway and an evaluation method of the jellyfish and snow rabbits, and relates to the technical field of biological medicines.The method comprises the following steps that 1, a treated jellyfish and snow rabbits breast cancer cell group and a control breast cancer cell group are established; and step 2, respectively dividing the two groups of samples in the step 1 into two parts, fixing one part in paraformaldehyde with the mass concentration of 10%, treating, embedding in paraffin for immunohistochemical analysis, and quickly freezing the other part for transcriptomics analysis and the like. According to the invention, UPLC-MS is adopted to determine the main components of SMM, the influence of SMM on breast cancer (BC) is evaluated through in-vivo experiments, in order to deeply clarify the mechanism, network pharmacology and transcriptomics analysis are integrated, finally, immunohistochemistry (IHC) is adopted to verify and explore the exact breast cancer resisting mechanism of SMM, and a scientific basis is provided for potential clinical application of SMM.

The invention discloses a traditional Chinese medicine composition and application thereof in medicine for improving non-alcoholic fatty liver, and the traditional Chinese medicine composition comprises pulvis fellis suis, pericarpium citri reticulatae, hawthorn and clostridium butyricum. On one hand, potential targets and pathways of drug diseases are determined in network pharmacological analysis, and on the other hand, in an in-vivo experiment, the drug provided by the invention improves obesity and impaired glucose tolerance caused by HFD, reduces the blood fat level, alleviates inflammation, protects the liver, alleviates liver fat vacuole deposition and fatty degeneration, and has a good application prospect. It is proved that the bile acid-hawthorn and pericarpium citri reticulatae-clostridium butyricum combination has prevention and treatment and protection effects on the HFD-induced non-alcoholic fatty liver disease.

The invention discloses a network pharmacology and Mendel randomization analysis method for resisting lithangiuria by chlorogenic acid, and belongs to the technical field of biomedical engineering, and the method comprises the following steps: taking an intersection of a potential action target of chlorogenic acid and a lithangiuria target as a candidate target; performing pathway enrichment analysis and screening on each candidate target spot to determine a plurality of first pathways; for each first path, calculating a target importance score of each candidate target and performing screening according to the target importance score, and obtaining the screened candidate target as a key target; respectively performing Mendel stochastic analysis on each key target spot to obtain a causal effect estimated value of each key target spot and the urinary calculus risk, screening all the first pathways according to the causal effect estimated value, taking the screened first pathways as target pathways, and taking the key target spots in the target pathways as target targets. The method can be used for efficiently and accurately screening and determining the core regulatory pathway playing the anti-lithangiuria role and the real key target to which the core regulatory pathway belongs.

The invention belongs to the technical field of biological medicine, and particularly relates to application of LRRFIP1 serving as a bilberry extract to improvement of a target spot of insulin resistance of gestational diabetes mellitus. The insulin resistance of gestational diabetes mellitus is improved by specifically regulating and controlling a PI3K-AKT signal channel and a GLUT4 glucose transporter function in skeletal muscle based on the bilberry extract. The bilberry extract used in the invention is a natural plant source, is high in safety, and is suitable for pregnancy. PI3K-AKT signal channels can be activated at the same time, and insulin resistance and lipid metabolism disorder can be synergistically improved. The action mechanism is systematically illuminated through technical means such as proteomics, network pharmacology, molecular docking and the like. Glucose tolerance, insulin sensitivity and blood fat level of GDM rats can be remarkably improved, and no remarkable side effect exists.

The invention provides a screening method and application of external traditional Chinese medicine anti-psoriasis active ingredients, and relates to the technical field of modern research of traditional Chinese medicines. According to the screening method disclosed by the invention, components with transdermal ability and anti-psoriasis activity are systematically excavated and screened from a traditional Chinese medicine complex system through preparation of a traditional Chinese medicine transdermal sample solution, UPLC-Q-TOF-MS component identification, network pharmacological analysis, molecular docking prediction and HaCaT psoriasis cell model verification in sequence. The method solves the problems that the traditional Chinese medicine transdermal components are unclear in pharmacodynamic material basis and low in screening efficiency, and has the advantages of being high in systematicness, high in accuracy and good in pertinence. According to the method, a plurality of effective components such as dictamnine and limonin are successfully screened from the cortex dictamni, and a clear pharmacological basis is provided for research and development of the cortex dictamni externally applied anti-psoriasis medicine.

The invention discloses a traditional Chinese medicine composition for preventing and treating avian pathogenic escherichia coli as well as a preparation method and application thereof, and relates to the technical field of veterinary drugs. According to the invention, screening is carried out through subtraction genomics and reverse network pharmacology, and based on the compatibility theory of monarch, minister, assistant and guide and the traditional Chinese veterinary medicine and pharmacy differentiation theory, a traditional Chinese medicine composition, namely the radix angelicae and fructus forsythiae intestine health formula, for preventing and treating avian pathogenic escherichia coli is formed. According to the traditional Chinese medicine composition, fructus forsythiae and radix angelicae are used as monarch drugs, radix isatidis and clinopodium polycephalum are used as ministerial drugs, fructus aurantii immaturus, mint and plantain herb are used as adjuvant drugs, and liquorice is used as a conductant drug, so that the traditional Chinese medicine composition can inhibit reproduction of avian pathogenic escherichia coli, destroy formation of biological membranes of the avian pathogenic escherichia coli and relieve pathological injuries of diseased poultry caused by APEC; the traditional Chinese medicine composition has a good treatment effect on avian pathogenic escherichia coli.

The invention relates to a screening method of anti-breast cancer key targets of a traditional Chinese medicine composition for treating triple-negative breast cancer pulmonary metastasis, which sequentially comprises the following steps: component-target preliminary screening, two sample Mendel randomization, SMR and co-localization, full phenotype association and side effect evaluation, pathway enrichment, clinical expression verification, molecular docking verification and the like. After Mendel randomization (MR) and SMR are placed in network pharmacology, population genetic variation is used as a natural randomization tool, evidence of causal relationship between exposure (target level) and outcome (breast cancer risk) is provided on a public GWAS level, traditional association analysis mixing and reverse causal risk are reduced, and the false positive rate of network pharmacology prediction is greatly reduced; pheWAS-MR is introduced to perform system identification on phenotypes of various diseases, so that a potential off-target effect can be identified in advance, the multiple effects and possible adverse reactions of potential treatment targets in traditional Chinese medicine compound targets can be evaluated, powerful data support is provided for subsequent clinical test design and drug alert strategies, and the risk of later failure of research and development is effectively reduced.

The invention belongs to the technical field of medicinal material screening, and relates to a traditional Chinese medicine compound for treating attention deficit hyperactivity disorder and a screening method thereof. The screening method comprises the following steps: constructing a drug use database of ADHD; performing statistics and association rule analysis on frequency, property and flavor tropism and efficacy of the traditional Chinese medicines to obtain a high-frequency traditional Chinese medicine composition; screening ADHD disease targets and establishing a target set; performing clustering analysis and integration on the high-frequency traditional Chinese medicine composition to obtain a core medicine composition; constructing candidate traditional Chinese medicine compounds through reverse network pharmacological analysis; evaluating the efficacy of the candidate traditional Chinese medicine compound through animal experiments, and screening out the optimal traditional Chinese medicine compound. According to the method, an O-R-S analysis mode is constructed through data mining and reverse network pharmacology, traditional Chinese medicine compound screening and evaluation processes of ADHD and candidate compounds with better comprehensive performance are optimized, and a reference scheme with data support and mechanism basis is provided for traditional Chinese medicine clinical treatment and new drug research and development of ADHD.

The invention discloses a traditional Chinese medicine composition for inhibiting salmonella choleraesuis as well as a preparation and application thereof, and belongs to the technical field of traditional Chinese medicine and network pharmacology. According to the invention, traditional Chinese medicines with a good antibacterial effect on S. Choletaesuis are screened, and the compound five-plum decoction with a good antibacterial effect on S. Choletaesuis is established. The five-plum decoction can be used for effectively regulating and controlling the steady state of intestinal flora, and reducing the oxidative damage and inflammatory damage of the S. Cholera to the intestinal tract and the liver by regulating and controlling the signal channels of TLR4 / NF-kB / MyD88 and ZO-1 / Claudin-1 / Occludin. Through the multiple effects of resisting bacteria, resisting inflammation, protecting intestinal barriers and improving production performance, the five-plum decoction shows remarkable prevention and treatment effects on swine paratyphoid, and has huge value of being developed into a novel veterinary traditional Chinese medicine preparation and put into clinical application.

This invention provides a method for screening the active ingredient groups of Xinsu Ning capsules, relating to the technical field of quality control and pharmacodynamic substance analysis of traditional Chinese medicine. The screening method for the active ingredient groups of Xinsu Ning capsules provided by this invention integrates non-targeted chemical component analysis, in vivo prototype and metabolite detection, network pharmacology correlation analysis, and fingerprint spectrum construction, enabling a systematic evaluation of the in vivo exposure characteristics, target regulatory potential, and quality consistency of candidate components. This method can identify chemical components related to pharmacodynamic effects from complex systems, providing an operable technical path for the quality control and pharmacodynamic substance basis research of Xinsu Ning capsules.

The invention relates to a big data screening method of a traditional Chinese medicine compound for treating enteritis and application thereof, and belongs to the field of traditional Chinese medicine screening based on a data mining technology, animal experiment efficacy investigation and network pharmacology prediction. Comprising the following steps: performing data arrangement specification on a traditional Chinese medicine compound for treating enteritis based on a data mining technology; analyzing and summarizing the standardized data to obtain a new traditional Chinese medicine compound (AEND); constructing an acute enteritis mouse model, and performing pharmacodynamic evaluation on the traditional Chinese medicine compound; predicting the action mechanism of the screened traditional Chinese medicine compound based on a network pharmacology method; a molecular docking technology is applied to virtually verify the action target of the traditional Chinese medicine compound on acute enteritis; the pathway protein of a mammalian cell receptor tyrosine kinase mediated phosphatidylinositol related (PI3K / AKT) signal pathway is detected. According to the invention, a new formula AEND is screened out based on data mining, and a pharmacological experiment verifies that the AEND has a protective effect on mice with acute enteritis. The developed traditional Chinese medicine compound has the effects of resisting inflammation and regulating the homeostasis of intestinal flora.

The invention discloses a method for analyzing the action mechanism of a decoction of trichosanthes kirilowii maxim, allium macrostemon and pinellia ternate for treating heart failure (heart failure), and the multi-component, multi-target and multi-channel synergistic action mechanism of the prescription is systematically analyzed by integrating network pharmacology and metabonomics technologies. The method provides a systematic analysis strategy for research on a mechanism of treating complex diseases by a traditional Chinese medicine compound.

The invention relates to the technical field of traditional Chinese medicine quality control, and particularly discloses a traditional Chinese medicine compound preparation component identification method which comprises the following steps: taking a traditional Chinese medicine compound preparation, grinding and uniformly mixing to obtain powder; adding the powder into an extraction solvent, performing ultrasonic extraction and centrifugation, and taking supernate; component analysis is carried out on the supernate by adopting chromatography and mass spectrometry detection, and the chromatographic conditions are as follows: the chromatographic column is Thermo Hypersil gold C18 (1.9 [mu] m, 2.1 mm * 100 mm); the flow velocity is 0.3 mL / min, and the sample size is 10 [mu] L; mobile phases are as follows: a phase A is a 0.1% formic acid aqueous solution, a phase B is a 0.1% formic acid acetonitrile solution, and gradient elution is 0-1 min, 90% of A; in 1-15 min, 90%-10% of A is obtained; 15-17 min, 10% of A is taken; in 17-17.1 min, 10% to 90% of A is obtained; according to the component identification method of the traditional Chinese medicine compound preparation, qualitative analysis is conducted on the traditional Chinese medicine compound preparation through an LC-MS method, a basis and support are provided for quality control of the traditional Chinese medicine compound preparation, and a good foundation is provided for network pharmacology research.

The invention belongs to the technical field of network pharmacology and molecular docking, and discloses a method for analyzing treatment of neurodegenerative diseases by astaxanthin based on network pharmacology and molecular docking technologies. Comprising the following steps: acquiring a target corresponding to an astaxanthin active component, collecting a neurodegenerative disease related target, constructing an astaxanthin-neurodegenerative target PPI network, performing enrichment analysis on a core target GO and KEGG, and performing molecular docking. The research is based on an integrated pharmacology platform, a mechanism of astaxanthin for treating neurodegenerative diseases is explored through molecular docking, and an action mechanism of astaxanthin for treating neurodegenerative diseases and related target genes are found and predicted.

The invention discloses application of a pharmaceutical composition in preparation of a medicine for treating Alzheimer's disease, and belongs to the technical field of biological medicine. The pharmaceutical composition is a mixture of gastrodia elata total glycosides and uncaria total alkaloids. The mass ratio of the gastrodia elata total glycosides to the uncaria total alkaloids is 3: 4. The pharmaceutical composition provided by the invention shows a synergistic protection effect on A beta-induced HT22 neuron injury, the effects of improving cell viability, improving cell morphology and inhibiting cell apoptosis are remarkably superior to those of a single component, the limitation that the curative effect of an existing single-target anti-AD drug is poor is effectively overcome, and a multi-target synergistic nerve protection effect is achieved. Furthermore, by means of network pharmacology and molecular docking technologies, it is illuminated from the system level that the composition synergistically forms a regulation network covering a plurality of AD core pathological targets such as BACE1, GSK3beta and AChE, and the bottleneck that a traditional Chinese medicine compatibility mechanism is unknown is broken through.

The application discloses a research method of a potential protein target point of Guangxi Yaoshan sweet tea against diabetic nephropathy based on proteomics, relates to the technical field of biological medicine, and solves the problem of lacking research on the target point of the action of Yaoshan sweet tea on the prevention and treatment of diabetic nephropathy from the perspective of proteomics. The method comprises the following steps: determining a characteristic active ingredient, namely myricetin, of Yaoshan sweet tea for intervention on diabetic nephropathy, obtaining a PPI network of network pharmacology of the intersection target point of the characteristic active ingredient of Yaoshan sweet tea and diabetic nephropathy; preparing a myricetin solution, preparing a cell disease model and grouping intervention, collecting and pre-treating a cell protein sample, performing mass spectrometry, pre-processing proteomic mass spectrometry data, screening a potential protein target point, identifying and analyzing an expression change trend of the potential protein target point, and analyzing a core protein target point and a key signal path. The application directly locates the key protein target point of Guangxi Yaoshan sweet tea on diabetic nephropathy from a protein expression level, which is more intuitive and more accurate.

The invention belongs to the technical field of medicines, and particularly relates to application of a licorice extract in preparation of a medicine for treating premature ovarian failure. On the basis of network pharmacology prediction and animal experiment verification, through experimental exploration, it is clear that diammonium glycyrrhizinate can treat premature ovarian failure by improving ovarian tissue energy metabolism, reducing the oxidative stress level and inhibiting ovarian cell apoptosis, and also can treat premature ovarian failure by improving the ovarian growth and development microenvironment. According to the present invention, the growth and development rate of ovarian granular cells and membrane cells is improved, the sufficient quantity of granular cells and membrane cells are ensured to secrete female progestational hormone, the damaged ovarian tissue is further protected, the new strategy and hope are provided for the treatment of premature ovarian failure, and the pharmaceutical composition is expected to become the safe and effective treatment drug.

The invention discloses a compound composition based on a Gegen Qinlian decoction, and belongs to the technical field of medicines. The active ingredients of the compound composition comprise puerarin, baicalin, berberine, glycyrrhizic acid and paeoniflorin, and the compound composition comprises pharmaceutically acceptable auxiliary materials. The daily dose of the compound composition is prepared from the following components in parts by mass: 50 to 300 parts of puerarin, 50 to 800 parts of baicalin, 50 to 800 parts of berberine, 25 to 300 parts of glycyrrhizic acid and 30 to 150 parts of paeoniflorin. The compound composition is composed of five clear active components, and through accurate control and standardized quality control of the active components, the stability of the product quality and the reliability of the curative effect are fundamentally ensured. Network pharmacological analysis and animal experiment results show that the composition has antipyretic, anti-inflammatory and analgesic effects, is matched with the traditional Chinese medicine effects of relieving muscles and relieving exterior syndrome, clearing away heat and toxic materials, and promoting diuresis and stopping dysentery, and is suitable for treating stomachache, headache and body pain.

The invention provides a traditional Chinese medicine composition, a traditional Chinese medicine preparation, a preparation method and application, and particularly belongs to the technical field of traditional Chinese medicine preparation. The traditional Chinese medicine composition comprises the following components in parts by weight: 7-11 parts of champignon, 19-29 parts of aloe, 10-14 parts of fructus chebulae immaturus, 5-7 parts of rhizoma zingiberis, 5-7 parts of rhizoma nardostachyos, 4-6 parts of fructus chebulae and 2-4 parts of halite. The traditional Chinese medicine composition can improve the activity of SOD, inhibit the content of MDA, reduce damage of oxygen free radicals to gastric mucosa, effectively relieve inflammatory response, achieve anti-inflammation, improve ulcer surface blood flow, improve gastric mucosa damage, reduce ulcer surfaces and promote ulcer healing, and therefore the protective effect on gastric ulcer is achieved. The results of network pharmacology, molecular docking speculation and Western blot method verification show that the FMST plays an anti-inflammatory role through a TLR4 / MyD88 / NF-kappa B signal channel.

The application discloses a Hypsizygus marmoreus extract and a preparation method and application thereof. The extract is prepared from the Hypsizygus marmoreus through a method combining water extraction, alcohol precipitation, macroporous adsorption resin and silica gel column chromatography, UPLC-ESI-MS / MS analysis identifies 830 compounds, and 26 main compound components with a relative content of more than 0.2%; network pharmacology analysis obtains 40 active compounds and 23 T2MD related core target points. The extract can significantly reduce fasting blood glucose and postprandial blood glucose, can significantly reduce serum total cholesterol, triglyceride and low-density lipoprotein cholesterol levels, can improve fatty liver level, can significantly reduce serum inflammatory factors IL-6, TNF-alpha and CRP levels, can significantly increase GLP-1 and serum insulin levels, and can improve intestinal flora alpha diversity, improve beta diversity structure and restore the abundance of beneficial bacteria. The Hypsizygus marmoreus extract has multi-target and multi-metabolic regulation functions, can be used for developing a blood glucose and blood lipid reducing medicine or health food, and has high safety and definite effect.

The invention belongs to the technical field of biological computer analysis, and discloses an analysis method based on network pharmacology and a molecular docking technology. The psoriasis treatment of fucoidin is analyzed on the basis of network pharmacology and a molecular docking technology, five core targets, 30 GO annotations and 66 KEGG pathways are screened through the network pharmacology and the molecular docking technology, and the affinity of active ingredients and the core targets is good.

The invention discloses application of steroid saponin of trillium tschonoskii maxim to preparation of a medicine for treating vascular dementia, and relates to the technical field of medicines. Animal experiments prove that the berberis apiacea steroid saponin can improve the spatial reference learning function of cerebral ischemia rats, improve the spatial reference memory function of the cerebral ischemia rats and enhance the long-term memory function of the cerebral ischemia rats. In addition, brain tissue damage and white matter structure damage of rats suffering from cerebral ischemia can be relieved, axonal myelin sheath microstructure reconstruction and white matter fiber reconstruction of the rats suffering from cerebral ischemia are promoted, and myelin sheath regeneration of rats suffering from vascular dementia is promoted. Network pharmacological analysis finds that the age grass total steroid saponin has a wide regulation effect on OPCs and OLs cell components and molecular functions, and further, OPCs differentiation myelin sheath inhibition related factors are regulated and controlled, and white matter myelin sheath regeneration is promoted.

The invention discloses an action mechanism analysis method of an external medicine for treating liver cancer ascites, and relates to the technical field of medicines. Comprising the following steps: screening active ingredients and related targets of a medicine through network pharmacology; predicting liver cancer ascites related disease targets; constructing an intersection target point network of active ingredient target points and disease target points, and performing protein interaction network analysis; carrying out GO function and KEGG pathway enrichment analysis on the intersection target spot; establishing a medicine, component, disease, target spot and pathway network; the binding activity of the core target and the active component is verified through molecular docking; establishing a liver cancer ascites model through animal experiments, and performing efficacy verification; network pharmacology and animal experiment data are integrated, and a drug action mechanism is analyzed. According to the invention, active ingredients, target spots and pathways of the medicine are rapidly screened through network pharmacology, so that the earlier-stage research period is greatly shortened; animal experiment design is standardized, samples can be processed in batches, and the method is suitable for high-throughput drug screening.

The invention provides a network pharmacology-based large model generated traditional Chinese medicine prescription curative effect evaluation method and system, and the method comprises the steps: employing a machine learning model to generate candidate prescriptions, and obtaining all known active components of each traditional Chinese medicine in the prescriptions; predicting human body protein targets corresponding to the patient based on the known active components, and gathering all the human body protein targets to obtain a prescription target set P; obtaining human body protein targets related to the corresponding diseases, and gathering all the human body protein targets corresponding to the diseases to obtain a disease target set D; constructing a protein-protein interaction network; inputting the prescription target point set P and the disease target point set D into a protein-protein interaction network, and constructing a connected sub-network; calculating curative effect indexes of the connected sub-networks; and optimizing the prescription based on the curative effect index to complete the curative effect evaluation of the traditional Chinese medicine prescription. According to the method, calculation and experiments are closely combined, the blindness and the cost of experimental verification are remarkably reduced through a calculation priority strategy, and the research and development efficiency is improved.

This invention discloses the application of glycyrrhizin, an effective extract of licorice, in the preparation of drugs for the prevention, improvement, or treatment of hepatocellular carcinoma. This invention is the first to discover that glycyrrhizin possesses significant anti-hepatocellular carcinoma activity, significantly inhibiting the proliferation of human liver cancer cells HepG2 and Huh7, and inducing cell morphological shrinkage, decreased adhesion, and cell death. Transcriptome sequencing and network pharmacology combined analysis showed that glycyrrhizin exerts its effects by regulating the PI3K-Akt signaling pathway and cell cycle pathway, and regulates 10 core targets, including CCNA2, PLK1, AURKA, AKT1, EGFR, CASP3, BIRC7, HK1, PAEP, and ALDH3A1. Molecular docking confirmed the stable binding of glycyrrhizin to these targets. TCGA clinical database validation showed that the expression levels of these core targets were significantly correlated with the prognosis of hepatocellular carcinoma patients. This invention clarifies for the first time the pharmacodynamics and molecular mechanism of glycyrrhizin against hepatocellular carcinoma, providing a safe, efficient, multi-target natural small molecule anti-liver cancer candidate drug with significant clinical translational and application value.